Method of controlling, limiting or preventing ascomycetes

Abstract

The present invention relates to a method of controlling, limiting or preventing the infestation of plants by Ascomycota plant pathogens, which comprises applying to the plant propagation material a pesticidal combination comprising as active ingredient A a phosphorus-based compound and as active ingredient B a fungicide selected from the group consisting of acibenzolar-S-methyl, fludioxonil, fluazinam, fluindapyr, metconazole, prothioconazole, silthiofam, triticonazole, flutianil, fluorofuram, azoxystrobin, difenoconazole, pyridylcarbamate, ipconazole, tetraconazole, silthiofam, dimethomorph, thiophanate-methyl, natamycin, benzene enofluorocarbostyril, fluazinam, triflupyrrole, high efficiency metalaxyl (metamitron), tetraconazole, isoxazoles, bromofluoride, thiamethoxam, imidacloprid, clothianidin, cyantraniliprole, tioxazofen, flufenoxuron and any mixtures thereof.

Description

[0001] This invention relates to a method for controlling, limiting, or preventing plant infection by plant pathogenic microorganisms (such as ascomycete plant pathogens) in the treatment of plant propagation material, and to seed treatment applications.

[0002] Although many fungicidal compounds and compositions belonging to various chemical classes have been developed or are being developed for use as fungicides in useful plant crops, crop tolerance and activity against specific plant pathogenic fungi do not always meet the needs of agricultural practice in many respects, especially as treatment of plant propagation material. Therefore, there is a persistent need to discover new compounds and compositions with superior biological properties for the control or prevention of plant infection by plant pathogenic fungi.

[0003] Therefore, the objective of this invention is to propose new mixtures, and in particular new treatments for plant propagation materials, thereby ensuring good efficacy against plant pathogenic fungi belonging to the Ascomycota phylum, and in particular to achieve a synergistic effect in treating plant pathogenic fungi belonging to the Ascomycota phylum.

[0004] Therefore, a first object of the present invention relates to a method for controlling, limiting, or preventing infection by plant pathogens of the Ascomycota phylum, the method comprising applying a pest-killing compound to the propagating material of the plant, the pest-killing compound comprising a phosphorus-based compound as active ingredient A and a mixture selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fluinbamectin, chlorfenapyr, prothioconazole, fluoxazin, thiamethoxam, fluoxapiprolin, fluoxastrobin, difenoconazole, fluopyram, tebuconazole, and tetrazole. The active ingredient B of acetamiprid, silthiamethoxam, dimethomorph, thiophanate-methyl, natamycin, benzo[a]flufenoxam, fluopyram, triflupyridine, metalaxyl-M (mefenoxam), tetrazolium pyridaben, isoxaflutole, bromfenoxam, thiamethoxam, imidacloprid, thiamethoxam, chlorantraniliprole, tetrazolium pyridaben, fipronil, and any mixture thereof, preferably an effective amount of active ingredient A and active ingredient B for fungicide purposes, optionally including one or more conventional formulation adjuvants.

[0005] In the context of this invention, control, limitation, or prevention (and variations thereof) means reducing any undesirable effects, such as pathogenic and especially plant pathogenic effects, such as infection or invasion of plant reproductive material by fungi belonging to the Ascomycota phylum, and pathogenic damage to plant reproductive material, to a level that shows improvement.

[0006] The method according to the invention has the advantageous property of protecting plants from pathogenic (such as plant pathogenic), especially ascomycete plant pathogens, which cause plant diseases and damage; particularly in the case of plants, the invention can control, limit or prevent pathogenic damage to seeds, plant parts and / or plants grown from treated seeds.

[0007] The method according to the invention has a particularly favorable level of bioactivity against Ascomycota plant pathogens when used as plant propagation material.

[0008] The application of pest control combinations can be carried out in any desired order or simultaneously, and preferably simultaneously.

[0009] The phosphorus-based compounds according to the invention can be oxidized forms of phosphorus, such as phosphonates, and more particularly phosphonates or phosphonic acids. Phosphonates may preferably contain at least one functional group characterized by phosphorus atoms bonded to three oxygen atoms. The term "phosphonate" may also be referred to as "phosphite".

[0010] Phosphonates can be made from salts or esters of phosphorous acid (or phosphonic acid) and can include alkyl phosphonates.

[0011] More specifically, the phosphorus-based compound can be a phosphonate, and even more specifically, a phosphonate of potassium, sodium, calcium, aluminum, or ammonium.

[0012] The phosphorus-based compound may be selected from phosphonic acids, phosphonates (e.g., potassium phosphonate, sodium phosphonate, calcium phosphonate, and / or ammonium phosphonate), other phosphorus-based compounds, and any mixture thereof; and preferably, the phosphorus-based compound may be selected from phosphonic acids, one or more phosphonates, and any mixture thereof. For example, the phosphorus-based compound may be selected from phosphorus pentoxide, dipotassium phosphonate, monopotassium phosphonate, disodium phosphonate, calcium phosphonate, ammonium methylphosphonate, monoammonium phosphonate, diammonium phosphonate, aluminum tri(ethylphosphonate), phosphonic acid, and any mixture thereof, and more preferably from phosphonic acid, monopotassium phosphite, dipotassium phosphite, monoammonium phosphonate, diammonium phosphonate, and any mixture thereof. The phosphorus-based compound may be a commercially available product, such as an industrial active ingredient, or a formulation containing an active ingredient.

[0013] Since the term "phosphonate" can also be called "phosphite", the descriptions of dipotassium phosphonate, monopotassium phosphonate, disodium phosphonate, calcium phosphonate, ammonium methylphosphonate, monoammonium phosphonate, diammonium phosphonate, and aluminum tri(ethylphosphonate) can also be understood as dipotassium phosphite, monopotassium phosphite, disodium phosphite, calcium phosphite, ammonium methylphosphite, monoammonium phosphite, diammonium phosphite, and aluminum tri(ethylphosphonate).

[0014] The plants according to the present invention can be selected from vegetables (including Brassica, Solanaceae, Cucurbitaceae, leaves, roots and stems), legumes, oil crops, sugar beets, fiber plants and cereals, and are preferably selected from vegetables, legumes, oil crops and cereals.

[0015] In one particular embodiment, the vegetables may be selected from asparagus, eggplant, broccoli, cabbage, cauliflower, carrot, cucumber, garlic, zucchini, melon, okra, onion, pepper, potato, pumpkin, rhubarb, spinach, tomato, lettuce, chard, celery, chicory, romaine lettuce, and arugula. The vegetables may include leafy greens selected from lettuce, chard, spinach, celery, chicory, romaine lettuce, and arugula. Preferred vegetables may be broccoli, cabbage, cauliflower, carrot, pepper, tomato, lettuce, or spinach.

[0016] In another specific embodiment, the legume crop may be selected from beans, lentils, peas, and soybeans (e.g., yellow soybeans). Preferred legume crops may be yellow soybeans, peas, beans, or common beans.

[0017] In another specific embodiment, the oilseed crop may be selected from rapeseed, canola, mustard, poppy, olive, sunflower, coconut, castor bean, cocoa, and peanut. Preferred oilseed crops may be rapeseed, canola, or sunflower.

[0018] In another specific embodiment, the beet may be selected from sugar beet, feed beet and red beet.

[0019] In another specific embodiment, the fiber plant may be selected from cotton, flax, hemp, and jute.

[0020] In another specific embodiment, the grain may be selected from wheat, barley, rye, oats, and corn, with corn being a preferred grain. Corn may be selected from field corn, popcorn, corn, rice, sorghum, and related crops. Preferred grains may be wheat, rice, or corn.

[0021] In this invention, plant propagation material can be understood to refer to all reproductive parts of a plant that can be used for its propagation (such as seeds), including nutrient plant material such as cuttings. Seeds (strictly speaking), roots, fruits, tubers, bulbs, rhizomes, and plant parts can be mentioned as plant propagation material. The plant propagation material can be treated with one or more active ingredients of this invention before sowing or planting. Alternatively, the plant propagation material can be treated with one or more active ingredients of this invention during sowing or planting. Additionally, one or more active ingredients of this invention can be applied to the previously treated propagation material before or during planting. One or more active ingredients of this invention can be applied during seed sowing. These one or more active ingredients can also be used for plant propagation material derived from plants grown in greenhouses and / or during transplanting.

[0022] More preferably, the plant propagation material is plant seeds.

[0023] Seed treatment can occur on unsown seeds, and the term "unsown seeds" is intended to include seeds at any time between seed harvest and sowing in the land (for the purpose of plant germination and growth). Treatment of unsown seeds is not intended to include practices such as the application of pesticides to the soil, but rather to include any application practices performed on seeds during the sowing / planting process.

[0024] The treated plant propagation material of this invention can be treated in the same way as conventional plant propagation material. The treated plant propagation material can be stored, treated, sown, and cultivated in the same way as any other material treated with pesticides.

[0025] According to the present invention, active ingredients A and B may be present in synergistically effective amounts in the pest-killing composition.

[0026] In this invention, the application amount may be 1 to 500 grams of active ingredient A relative to 100 kg of plant propagation material, preferably 10 to 400 grams of active ingredient A, preferably 20 to 350 grams of active ingredient A, preferably 30 to 300 grams of active ingredient A, and more preferably 30 to 200 grams of active ingredient A.

[0027] The application amount can be 0.5 g to 500 g of active ingredient B relative to 100 kg of plant propagation material, preferably 1 g to 500 g of active ingredient B, preferably 1 g to 400 g of active ingredient B, preferably 1 g to 300 g of active ingredient B, preferably 1 g to 200 g of active ingredient B, preferably 1 g to 100 g of active ingredient B, preferably 1 g to 75 g of active ingredient B, preferably 1 g to 50 g of active ingredient B, and more preferably 1 g to 30 g of active ingredient B.

[0028] In one specific embodiment, the application amounts of active ingredient A and active ingredient B (more preferably for Fusarium diseases, Septoria diseases, and / or Pyricularia diseases) can be as follows:

[0029]

[0030]

[0031] For example, the following amounts can be applied, more preferably, to Fusarium diseases, Phyllostachys diseases, and / or Pyreospora diseases:

[0032]

[0033] In another specific embodiment, the application amount may advantageously include a weight ratio of active ingredient A to active ingredient B of 1:100 to 1000:1, preferably 1:100 to 400:1, preferably 1:100 to 100:1, preferably 1:10 to 10:1, preferably 1:5 to 5:1, and more preferably 1:2 to 2:1.

[0034] More preferably, the application amount may advantageously include a weight ratio of active ingredient A to active ingredient B of 1:1 to 1:100, preferably 1:1 to 1:50, preferably 1:1 to 1:10, preferably 1:1 to 1:5, and more preferably 1:1 to 1:2; or the application amount may advantageously include a weight ratio of active ingredient A to active ingredient B of 100:1 to 1:1, preferably 50:1 to 1:1, preferably 10:1 to 1:1, preferably 5:1 to 1:1, and more preferably 2:1 to 1:1.

[0035] In another specific embodiment, the pest control combination may comprise a third active ingredient C, different from the selected active ingredient A and the selected active ingredient B, wherein active ingredient C is selected from phosphorus pentoxide, dipotassium phosphonate, monopotassium phosphonate, disodium phosphonate, calcium phosphonate, ammonium methylphosphonate, monoammonium phosphonate, diammonium phosphonate, aluminum tri(ethylphosphonate), phosphonic acid, aramidobenzene-S-methyl, fludioxonil, fluopyram, fludioxonil, chlorfluazuron, prothioconazole, and fluazole. Cyclosulfuron, tebuconazole, fluoxastrobin, fluoxastrobin sulfadiazine, azoxystrobin, difenoconazole, fluopyram, tebuconazole, tetrazalofop-p-ethyl, silthiamethoxam, dimethomorph, thiophanate-methyl, natamycin, benzo[a]fluopyram, fluoxastrobin hydroxylamine, triflupyridamole, metalaxyl-M (Methamphetamine), tetrazalofop-P-ethyl, isoxaflutole, bromfenoxam, thiamethoxam, imidacloprid, thiamethoxam, bromuconazole, chlorantraniliprole, tetrazalofop-P-ethyl, and fipronil. The pest control combination may preferably contain an effective amount of active ingredient C. When active ingredient C is selected from phosphorus pentoxide, dipotassium phosphonate, monopotassium phosphonate, disodium phosphonate, calcium phosphonate, ammonium methylphosphonate, monoammonium phosphonate, diammonium phosphonate, aluminum tri(ethylphosphonate), and phosphonic acid, the application amount of active ingredient C can be as defined in this invention for active ingredient A; or when active ingredient C is selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fluinbazin, chlorfluazuron, prothioconazole, fluoxastrobin, cymoxanil, tebuconazole, fluoxastrobin, fluoxastrobin sulfadiazine, pyrimethanil, etc. When using fungicides such as pyraclostrobin, difenoconazole, fluopyram, styraclostrobin, tetrazolium, silthiamethoxam, dimethomorph, thiophanate-methyl, natamycin, benzo[a]fluopyram, fluopyram, triflupyridine, metalaxyl (metalaxyl-M), tetrazolium pyridaben, isoxaflutole, bromfenoxam, thiamethoxam, imidacloprid, thiamethoxam, chlorantraniliprole, tetrazolium pyridaben, or fipronil, the application amount of active ingredient C can be as defined for active ingredient B in this invention.

[0036] In this invention, ascomycetes are plant pathogenic diseases, and can be selected from Fusarium diseases, Syngonium diseases, and Pyridia diseases. Infection of plants can cause various diseases and symptoms, including vascular wilt, seedling blight, root and stem rot, crown rot, damping-off, branch blight, yellowing, dwarfing, and seedling death.

[0037] For example, Fusarium diseases are caused by species of the genus Fusarium, including *Fusarium culmorum*, *Fusarium graminearum*, *Fusarium langsethiae*, *Fusarium moniliforme*, *Fusarium oxysporum*, *Fusarium poae*, *Fusarium proliferatum*, *Fusarium sacchari*, *Fusarium sambucinum*, *Fusarium subglutinans*, *Fusarium solani*, *Fusarium sporotrichioides*, *Fusarium tricinctum*, and *Fusarium wildigans*. The Cuban variants of *Fusarium virguliforme*, *Fusarium avenaceum*, *Fusarium fujikuroi*, *Fusarium subglutinans*, and *Fusarium oxysporum*.

[0038] In a preferred embodiment, the Fusarium disease may be Fusarium chrysogenum.

[0039] For example, diseases caused by *Septoria* species include *Septoria alatritici*, *Septoria nodorum*, and *Septoria glycines*.

[0040] In a preferred embodiment, the disease caused by *Syngonium* can be *Syngonium oryzae*.

[0041] For example, diseases caused by the genus Pyricularia are caused by species of the genus Pyricularia, including Pyricularia oryzae (also known as Magnaporthe oryzae or Magnaporthe grisea) and Magnaporthe oryzae Triticum (MoT).

[0042] In a preferred embodiment, the pyriform disease can be *Pyriformis oryzae*.

[0043] In one specific embodiment, the following combination of active ingredients A and B can be used to combat Fusarium diseases, and especially Fusarium oxysporum:

[0044] - Active ingredient A, selected from phosphorus pentoxide, dipotassium phosphonate, monopotassium phosphonate, disodium phosphonate, calcium phosphonate, ammonium methylphosphonate, monoammonium phosphonate, diammonium phosphonate, aluminum tri(ethylphosphonate), and phosphonic acid; preferably selected from phosphonic acid, monopotassium phosphite, dipotassium phosphite, and diammonium phosphonate; and more preferably selected from monopotassium phosphonate and phosphonic acid; and

[0045] -Active ingredient B is selected from aramid-type benzyl-S-methyl, fludioxonil, fluopyram, fluinbazin, chlorfenapyr, prothioconazole, fluoxastrobin, cymoxanil, fluoxastrobin, fluoxastrobin sulfadiazine, azoxystrobin, difenoconazole, fluopyram, tebuconazole, tetrazoxystrobin, dimethomorph, thiophanate-methyl, natamycin, benzo[a]fluoroquinolones, fluopyram, trifluoperamide, metalaxyl-M (high-efficiency metalaxyl-M), and tetrazoxystrobin. Ester, isoxaflutole, bromoxynil, thiamethoxam, imidacloprid, thiamethoxam, bromoxynil, chlorantraniliprole, tetrazolium, and fipronil; and preferably selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fluinbamil, chlorfluazuron, prothioconazole, fluoxastrobin, cymoxanil, fluoxastrobin, fluopyram, fluopyram, tetrazolium, benzo[a]fluoroquinolones, fluopyram, fluopyram, tetrazolium, benzo[a]fluoroquinolones, fluopyram, and triflupyridine.

[0046] In another specific embodiment, the following combination of pest control ingredients comprising active ingredient A and active ingredient B can be used to combat diseases of the genus *Syngonium*, and especially *Syngonium oryzae* of wheat:

[0047] - Active ingredient A, selected from phosphorus pentoxide, dipotassium phosphonate, monopotassium phosphonate, disodium phosphonate, calcium phosphonate, ammonium methylphosphonate, monoammonium phosphonate, diammonium phosphonate, aluminum tri(ethylphosphonate), and phosphonic acid; preferably selected from phosphonic acid, monopotassium phosphite, dipotassium phosphite, and diammonium phosphonate; and more preferably selected from dipotassium phosphonate, monopotassium phosphonate, and diammonium phosphonate; and

[0048] -Active ingredient B is selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fluinbazin, chlorfenapyr, prothioconazole, fluoxastrobin, cymoxanil, fluoxastrobin, fluoxastrobin sulfadiazine, azoxystrobin, difenoconazole, fluopyram, tebuconazole, tetrazoxystrobin, dimethomorph, thiophanate-methyl, natamycin, benzo[a]fluopyram, fluopyram hydroxylamine, triflupyridine, metalaxyl (high-efficiency metalaxyl), and tetrazoxystrobin. Ester, isoxaflutole, bromoxynil, thiamethoxam, imidacloprid, thiamethoxam, bromoxynil, chlorantraniliprole, tetrazolium, and fipronil; and preferably selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fluinbamil, chlorfluazuron, prothioconazole, fluoxastrobin, cymoxanil, fluoxastrobin, fluopyram, fluopyram, tetrazolium, benzo[a]fluoroquinolones, fluopyram, fluopyram, tetrazolium, benzo[a]fluoroquinolones, fluopyram, and triflupyridine.

[0049] In another specific embodiment, the following combination of pest-killing ingredients, comprising active ingredient A and active ingredient B, can be used to combat diseases of the genus *Pyrrosia*, and especially *Pyrrosia oryzae*:

[0050] - Active ingredient A, selected from phosphorus pentoxide, dipotassium phosphonate, monopotassium phosphonate, disodium phosphonate, calcium phosphonate, ammonium methylphosphonate, monoammonium phosphonate, diammonium phosphonate, aluminum tri(ethylphosphonate), and phosphonic acid; preferably selected from phosphonic acid, monopotassium phosphite, dipotassium phosphite, and diammonium phosphonate; and more preferably selected from dipotassium phosphonate, monopotassium phosphonate, and diammonium phosphonate; and

[0051] -Active ingredient B is selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fluinbazin, chlorfenapyr, prothioconazole, fluoxastrobin, cymoxanil, fluoxastrobin, fluoxastrobin sulfadiazine, azoxystrobin, difenoconazole, fluopyram, tebuconazole, tetrazoxystrobin, dimethomorph, thiophanate-methyl, natamycin, benzo[a]fluopyram, fluopyram hydroxylamine, triflupyridine, metalaxyl (high-efficiency metalaxyl), and tetrazoxystrobin. Ester, isoxaflutole, bromoxynil, thiamethoxam, imidacloprid, thiamethoxam, bromoxynil, chlorantraniliprole, tetrazolium, and fipronil; and preferably selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fluinbamil, chlorfluazuron, prothioconazole, fluoxastrobin, cymoxanil, fluoxastrobin, fluopyram, fluopyram, tetrazolium, benzo[a]fluoroquinolones, fluopyram, fluopyram, tetrazolium, benzo[a]fluoroquinolones, fluopyram, and triflupyridine.

[0052] According to the invention, one or more active ingredients may advantageously be in the form of a soluble concentrate (SL), a flowable concentrate (FS) for seed treatment, or a suspension concentrate (SC), and more preferably a seed treatment slurry applicable to seeds. Slurries for seed treatment application are well known in the art. For example, the slurry may contain one or more active ingredients (in the form of a commercially available product or a non-commercially available product), which are mixed with water and optionally with at least one polymer as described in the invention to optimize adhesion around the plant propagation material.

[0053] In the method of the present invention, one or more active ingredients may typically be in the form of a composition or formulation containing other conventional formulation adjuvants, as this allows for, for example, less cumbersome handling and application.

[0054] Each active ingredient can be applied separately to plant propagation material from different compositions, or these active ingredients can be aggregated in the same composition (premixed composition). The composition may contain one or more active ingredients in amounts from about 0.001% to about 99% by weight of the total composition. Suitablely, the composition contains one or more active ingredients in amounts from about 0.001% to about 50% by weight of the total composition. More suitablely, the composition contains one or more active ingredients in amounts from about 0.001% to about 10% by weight of the total composition. More suitablely, the composition contains one or more active ingredients in amounts from about 0.001% to about 1% by weight of the total composition. If the formulation is in concentrate form and requires dilution with water before use, it will contain a higher amount of active ingredient than a composition that can be used undiluted.

[0055] According to the present invention, one or more active ingredients can be used as pest control agents in unmodified form, but they are typically formulated into compositions in various ways using formulation adjuvants (such as liquid carriers (or solvents), solid carriers, and surfactants). Such formulations can be used directly or diluted before use. They can be diluted with, for example, water, liquid fertilizers, micronutrients, biological organisms, oils, and / or solvents.

[0056] These formulations can be prepared, for example, by mixing one or more active ingredients with a formulation adjuvant to obtain compositions in the form of finely dispersed solids, granules, solutions, dispersions, or emulsions. These active ingredients can also be formulated with other adjuvants, such as finely dispersed solids, mineral oils, oils of plant or animal origin, modified oils of plant or animal origin, organic solvents, water, surfactants, or any mixture thereof.

[0057] Adjuvants suitable for preparing compositions according to the invention are known in themselves.

[0058] As a liquid carrier, the following can be used: water, toluene, xylene, petroleum ether, vegetable oil, acetone, methyl ethyl ketone, cyclohexanone, acid anhydride, acetonitrile, acetophenone, amyl acetate, 2-butanone, butylene carbonate, chlorobenzene, cyclohexane, cyclohexanol, alkyl acetate, diacetone alcohol, 1,2-dichloropropane, diethanolamine, p-diethylbenzene, diethylene glycol, diethylene glycol rosinate, diethylene glycol butyl ether, diethylene glycol ethyl ether, diethylene glycol methyl ether, N,N Dimethylformamide, dimethyl sulfoxide, 1,4-dioxane, dipropylene glycol, dipropylene glycol methyl ether, dipropylene glycol dibenzoate, dipropylene glycol ether, alkylpyrrolidone, ethyl acetate, 2-ethylhexanol, ethylene carbonate, 1,1,1-trichloroethane, 2-heptanone, α-pinene, d-limonene, ethyl lactate, ethylene glycol, ethylene glycol butyl ether, ethylene glycol methyl ether, γ-butyrolactone, glycerol, glyceryl acetate, glyceryl diacetate, triacetic acid Glyceryl esters, hexadecane, hexanediol, isoamyl acetate, isobornyl acetate, isooctane, isophorone, cumene, isopropyl myristate, lactic acid, laurylamine, isopropylidene acetone, methoxypropanol, methyl isopentyl ketone, methyl isobutyl ketone, methyl laurate, methyl octanoate, methyl oleate, dichloromethane, m-xylene, n-hexane, n-octylamine, octadecanoic acid, octylamine acetate, oleic acid, oleyleneamine, o-xylene, phenol, polyethylene glycol, propionic acid, lactic acid Propylene acetate, propylene carbonate, propylene glycol, propylene glycol methyl ether, p-xylene, toluene, triethyl phosphate, triethylene glycol, xylenesulfonic acid, paraffin wax, mineral oil, trichloroethylene, perchloroethylene, ethyl acetate, amyl acetate, butyl acetate, propylene glycol methyl ether, diethylene glycol methyl ether, methanol, ethanol, isopropanol, and alcohols with higher molecular weights, such as pentanol, tetrahydrofurfuryl alcohol, hexanol, octanol, ethylene glycol, propylene glycol, glycerol, N-methyl-2-pyrrolidone, etc.

[0059] Solid carriers can be natural or synthetic solid materials that are insoluble in water. Such carriers are typically inert and acceptable in agriculture, particularly for treated seeds or other propagation materials. Examples include clay, diatomaceous earth, natural or synthetic silicates, titanium dioxide, magnesium silicate, aluminum silicate, talc, pyrophyllite clay, silica, attapulgite clay, diatomaceous earth, chalk, limestone, calcium carbonate, calcium montmorillonite, bentonite, bleaching clay, cottonseed hulls, wheat flour, soybean flour, pumice, wood flour, ground walnut shell powder, lignin, etc.

[0060] Many surfactants can be advantageously used in both solid and liquid formulations, especially those that can be diluted with a carrier before use. Surfactants can be anionic, cationic, nonionic, or polymeric, and they can be used as emulsifiers, wetting agents, suspending agents, or for other purposes. Typical surfactants include, for example, salts of alkyl sulfates, such as diethanolammonium dodecyl sulfate; salts of alkyl aryl sulfonates, such as calcium dodecylbenzenesulfonate; alkylphenol / olefin oxide addition products, such as ethoxylated nonylphenol; alcohol / olefin oxide addition products, such as ethoxylated tridecyl alcohol; soaps, such as sodium stearate; salts of alkyl naphthalene sulfonates, such as sodium dibutylnaphthalene sulfonate; salts of dialkyl sulfosuccinates, such as sodium di(2-ethylhexyl)sulfosuccinate; sorbitol esters, such as sorbitol oleate; quaternary ammonium, such as dodecyltrimethylammonium chloride; polyethylene glycol esters of fatty acids, such as polyethylene glycol stearate; block copolymers of ethylene oxide and propylene oxide; and salts of monoalkyl and dialkyl phosphate esters; and other substances, such as those described in McCutcheon's Detergents and Emulsifiers Annual, MC Publishing. Corp.), Ridgewood, New Jersey (1981).

[0061] Other adjuvants that can be used in formulations for killing harmful organisms include crystallization inhibitors, viscosity modifiers, suspending agents, dyes, antioxidants, foaming agents, light absorbers, mixing aids, defoamers, complexing agents, substances that neutralize or alter pH and buffer solutions, corrosion inhibitors, fragrances, humectants, absorption enhancers, micronutrients, plasticizers, flow aids, lubricants, dispersants, thickeners, antifreeze agents, microbial agents, and liquid and solid fertilizers.

[0062] The compositions according to the invention typically comprise from 1% to 99.9% by weight of a formulation adjuvant, which preferably comprises from 0% to 25% by weight of a surfactant. Commercial products may preferably be formulated as concentrates, while end users will typically use diluted formulations.

[0063] The compositions of the present invention may further comprise additional agrochemicals. These additional agrochemicals may be insecticides, fungicides, herbicides, synergists, plant growth regulators, nematicides, phytonutrients, plant fertilizers, and mixtures thereof.

[0064] During the treatment process, uniform distribution of the compositions of the present invention and their adhesion to the propagation material are desirable, and are particularly preferred when the propagation material is seeds. Treatment can range from a thin film (where the initial size and / or shape is identifiable) of a formulation containing the compositions of the present invention on plant propagation material (such as seeds) to a thick film (such as a coating or granules having many layers of different materials (such as a carrier, e.g., clay; different formulations, such as active ingredients; polymers; and colorants)) (where the initial shape and / or size of the seeds is no longer identifiable).

[0065] More specifically, the film may be a film-forming polymer well known in the art. The composition may contain at least one polymer selected from water-soluble and water-dispersible film-forming polymers. Suitable polymers have an average molecular weight of at least about 1,000 to at most about 100,000; more specifically, at least about 5,000 to at most about 100,000. Aqueous compositions typically contain about 0.5% to about 10% by weight of the polymer (b). In one specific embodiment, these compositions contain about 1.0% to at most about 5% by weight of the film-forming polymer. Suitable polymers are selected from random and block copolymers of alkyl oxidoxides, such as ethylene oxide-propylene oxide block copolymers (EO / PO block copolymers), including EO-PO-EO and PO-EO-PO block copolymers; ethylene oxide-butane random and block copolymers; C2-6 alkyl adducts of ethylene oxide-propylene oxide random and block copolymers; C2-6 alkyl adducts of ethylene oxide-butane random and block copolymers; polyoxyethylene-polyoxypropylene monoalkyl ethers, such as methyl ether, ethyl ether, propyl ether, butyl ether, or mixtures thereof; vinyl acetate / vinylpyrrolidone copolymers; alkylated vinylpyrrolidone copolymers; polyvinylpyrrolidone; polyalkylene glycols, including polypropylene glycol and polyethylene glycol; and any mixtures thereof. Specific examples of suitable polymers include Pluronic P103 (BASF) (EO-PO-EO block copolymer), Pluronic P65 (BASF) (EO-PO-EO block copolymer), Pluronic P108 (BASF) (EO-PO-EO block copolymer), Vinamul 18160 (National Starch) (polyvinyl acetate), Agrimer 30 (ISP) (polyvinylpyrrolidone), Agrimer VA7w (ISP) (vinyl acetate / vinylpyrrolidone copolymer), Agrimer AL 10 (ISP) (alkylated vinylpyrrolidone copolymer), PEG 400 (Uniqema) (polyethylene glycol), Pluronic R 25R2 (BASF) (PO-EO-PO block copolymer), Pluronic R 31R1 (BASF) (PO-EO-PO block copolymer), and Witconol NS. 500LQ (Witco) (Butanol PO-EO copolymer).

[0066] Therefore, one or more active ingredients of the present invention, and more preferably one or more compositions containing one or more active ingredients, can preferably adhere to reproductive materials (such as seeds).

[0067] While the method of the present invention is considered applicable to seeds in any physiological state, it is preferred that the seed be in a sufficiently stable state to be undamaged during the treatment. Typically, the seed is one that has been harvested from the field; removed from the plant; and separated from any rachis, stem, husk, and surrounding pulp or other non-seed plant material. The seed is also preferably biologically stable to ensure that the treatment does not cause biological damage to the seed. It is believed that the treatment can be applied to the seed at any time between seed harvesting and sowing, or during the sowing process (seed-directed application).

[0068] Conventional processing techniques and machinery, such as fluidized bed technology, drum milling methods, rotostatic seed processors, and drum coating machines, can be used to apply the compositions according to the invention to seeds. Other methods, such as spray beds, can also be useful. Seeds can be pre-sized before coating. After coating, the seeds are typically dried and then transferred to a sizing machine for sizing. Such procedures are known in the art and are described in more detail below.

[0069] The composition used in the method according to the invention may be referred to as a seed dressing composition, which relates to a liquid composition that can be used to at least partially or completely cover and / or moisten plant propagation material (and more preferably seeds).

[0070] The compositions according to the invention are particularly suitable for seed treatment of plant propagation materials, especially seeds. Seed treatment techniques are well known to those skilled in the art and can be readily applied in the context of this invention. The compositions of the invention are applied to seeds in the form of a slurry or soaking. Film coating or encapsulation, for example, may also be mentioned. Coating methods are well known in the art, and film coating or encapsulation techniques are used for seeds, while impregnation techniques are used for other propagation products.

[0071] A method of applying the composition according to the invention comprises spraying or wetting plant propagation material with an aqueous liquid formulation, or mixing the plant material with such a liquid formulation. Furthermore, prior to application, the composition of the invention can be diluted with water by simple mixing at ambient temperature to prepare an on-farm seed treatment formulation.

[0072] Typically, tank-mixed products for seed treatment application contain 0.25% to 80%, particularly 1% to 75% by weight, one or more active ingredients, and 99.75% to 20%, particularly 99% to 25% by weight, solid or liquid adjuvants (including, for example, solvents such as water), wherein these adjuvants may be surfactants, in amounts of 0% to 40%, particularly 0.5% to 30% by weight, based on the total weight of the tank-mixed product.

[0073] Typically, premixed preparations for seed treatment application comprise 0.5% to 99.9%, particularly 1% to 95% by weight, one or more active ingredients, and 99.5% to 0.1%, particularly 99% to 5% by weight, solid or liquid adjuvants (including, for example, solvents such as water), wherein these adjuvants may be surfactants, in amounts based on 0% to 50% by weight, particularly 0.5% to 40% by weight, of the total weight of the premixed preparation.

[0074] Commercial products will preferably be formulated as concentrates (e.g., premixed compositions (formulations)), while end users will typically use diluted formulations (e.g., drum mixes).

[0075] Preferred formulations may have the following composition (by weight%):

[0076] Emulsifiable concentrate :

[0077] Active ingredient: 1% to 95%, preferably 60% to 90%

[0078] Surfactant: 1% to 30%, preferably 5% to 20%

[0079] Liquid carrier: 1% to 80%, preferably 1% to 35%

[0080] Dust powder :

[0081] Active ingredient: 0.1% to 10%, preferably 0.1% to 5%

[0082] Solid carrier: 99.9% to 90%, preferably 99.9% to 99%.

[0083] Suspension concentrate:

[0084] Active ingredient: 5% to 75%, preferably 10% to 50%

[0085] Water: 94% to 24%, preferably 88% to 30%

[0086] Surfactant: 1% to 40%, preferably 2% to 30%

[0087] wettable powder :

[0088] Active ingredient: 0.5% to 90%, preferably 1% to 80%

[0089] Surfactant: 0.5% to 20%, preferably 1% to 15%

[0090] Solid carrier: 5% to 95%, preferably 15% to 90%

[0091] Granules:

[0092] Active ingredient: 0.1% to 30%, preferably 0.1% to 15%

[0093] Solid carrier: 99.5% to 70%, preferably 97% to 85%

[0094] Flowable concentrate for seed treatment :

[0095] Active ingredient: 1% to 75%, preferably 10% to 50%

[0096] Water: 98% to 24%, preferably 88% to 30%

[0097] Surfactant: 1% to 30%, preferably 2% to 20%

[0098] soluble concentrate :

[0099] Active ingredient: 1% to 95%, preferably 10% to 80%

[0100] Liquid carrier: 5% to 99%, preferably 20% to 90%

[0101] The following examples further illustrate (but do not limit) the invention.

[0102]

[0103] The combination is thoroughly mixed with an adjuvant and the mixture is thoroughly ground in a suitable grinder to obtain a wettable powder that can be diluted with water to obtain a suspension with a desired concentration.

[0104]

[0105] The combination is thoroughly mixed with the adjuvant and the mixture is thoroughly ground in a suitable grinder to obtain a powder that can be used directly for seed treatment.

[0106]

[0107] Emulsions with any required dilution that can be used in plant protection can be obtained from such concentrates by diluting them with water.

[0108]

[0109] A ready-to-use dust powder is obtained by mixing the mixture with a carrier and grinding the mixture in a suitable grinder. This type of powder can also be used for dry seed dressing.

[0110]

[0111] The mixture is combined with an adjuvant and ground, and then moistened with water. The mixture is extruded and then dried in an air stream.

[0112]

[0113] The finely ground mixture is then uniformly applied in a mixer to kaolin moistened with polyethylene glycol. This process yields dust-free coated granules.

[0114] suspension concentrate

[0115]

[0116] The finely ground mixture is tightly blended with an adjuvant to obtain a suspension concentrate, from which a suspension concentrate can be diluted with water to obtain a suspension with any desired dilution. Using such a dilution, living plants and plant propagation material can be treated and protected from microbial contamination by spraying, watering, or immersion.

[0117] Flowable concentrate for seed treatment

[0118]

[0119] The finely ground mixture is tightly blended with an adjuvant to obtain a suspension concentrate, from which a suspension concentrate can be diluted with water to obtain a suspension with any desired dilution. Using such a dilution, living plants and plant propagation material can be treated and protected from microbial contamination by spraying, watering, or immersion.

[0120] soluble concentrate

[0121]

[0122] Solutions with any required dilution that can be used in plant protection can be obtained by diluting this concentrate with water.

[0123] Sustained-release capsule suspension

[0124] 28 parts of the mixture were mixed with 2 parts of an aromatic solvent and 7 parts of a toluene diisocyanate / polymethylene-polyphenyl isocyanate mixture (8:1). This mixture was emulsified in a mixture of 1.2 parts of polyvinyl alcohol, 0.05 parts of an antifoaming agent, and 51.6 parts of water until the desired particle size was achieved. 2.8 parts of a 1,6-hexanediamine mixture in 5.3 parts of water were added to this emulsion. The mixture was stirred until polymerization was complete. The resulting capsule suspension was stabilized by adding 0.25 parts of a thickener and 3 parts of a dispersant. This capsule suspension formulation contained 28% of the active ingredient. The diameter of the media capsules was 8-15 micrometers. The resulting formulation was applied to seeds as an aqueous suspension suitable for this purpose.

[0125] Another object of the present invention is a plant propagation material, such as seeds, treated with a pest-killing combination as defined in the present invention.

[0126] Another object of the present invention is a pest-killing combination comprising:

[0127] - The phosphorus-based compound as active ingredient A is preferably selected from phosphorus pentoxide, dipotassium phosphonate, monopotassium phosphonate, disodium phosphonate, calcium phosphonate, ammonium methylphosphonate, monoammonium phosphonate, diammonium phosphonate, aluminum tri(ethylphosphonate), and phosphonic acid; and more preferably selected from phosphonic acid, monopotassium phosphite, dipotassium phosphite, and diammonium phosphonate; and

[0128] -Active ingredient B is selected from aramid-benzene-S-methyl, fludioxonil, fluopyram, fluinbamectin, chlorfenapyr, prothioconazole, fluoxastrobin, cymoxanil, fluoxastrobin, fluoxastrobin sulfadiazine, azoxystrobin, difenoconazole, fluopyram, styraclostrobin, tetrazoxystrobin, dimethomorph, thiophanate-methyl, natamycin, benzo[a]fluoroquinolones, fluopyram, trifluralin, metalaxyl (high-efficiency metalaxyl), tetrazoxystrobin, isoxazoline. Amines, bromfenac, thiamethoxam, imidacloprid, thiamethoxam, bromonitrile, chlorantraniliprole, tetrazolium, fipronil, and any mixture thereof; and preferably selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fluinbamil, chlorfluazuron, prothioconazole, fluoxastrobin, cymoxanil, fluoxastrobin, fluopyram, fluopyram, tetrazolium, benzoylfluopyram, fluopyram, triflupyridine, and any mixture thereof.

[0129] The pest control combination can be applied in any desired order or simultaneously.

[0130] In this invention, each active ingredient can be applied to the plant propagation material separately from different compositions, or these active ingredients can be aggregated in the same composition, such as, for example, a premixed composition or a barrel-mixed composition.

[0131] Each active ingredient can be applied separately from different compositions to plant propagation material, or these active ingredients can be aggregated in the same composition (premixed composition).

[0132] The pest control combination can be applied at the weight ratio of active ingredient A to active ingredient B as described in this invention.

[0133] Another object of the present invention is the use of the said pest-killing combination against Ascomycota plant pathogens, and more preferably against Fusarium diseases, Syndrome diseases and Pyridia diseases.

[0134] Another object of the present invention is a method for controlling, limiting or preventing plant infection, the method comprising applying the pest control combination to the growing site of the plant, the pest control combination preferably being resistant to Ascomycota plant pathogens, and more preferably resistant to Fusarium diseases, Phytosporum diseases and Pyridia diseases.

[0135] In this invention, as used herein, the term "site" means a field in which or on which plants grow, or a field in which seeds of cultivated plants are sown, or a field in which seeds are to be placed in soil. It includes soil, seeds and seedlings, and established vegetation.

[0136] In this invention, the above uses and methods may not include treating a human or animal body by surgical or therapeutic and diagnostic methods performed on the human or animal body. Example

[0137] The following non-limiting examples demonstrate the improved behavior associated with the method according to the invention.

[0138] A synergistic effect exists when the combined effect of active ingredients is greater than the sum of the effects of the individual components. For a given combination of active ingredients, the expected effect E follows the so-called Colby formula and can be calculated as follows (COLBY, SR, "Calculating synergistic and antagonistic responses of herbicide combination," Weeds, Vol. 15, pp. 20-22; 1967):

[0139] ppm = milligrams of active ingredient (= ai) per liter of spray mixture

[0140] X = % of the effect of active ingredient A, using p ppm of active ingredient.

[0141] Y = % of the effect of active ingredient B, using q ppm of active ingredient.

[0142] According to COLBY, using p + q ppm of active ingredients, the expected effect of the (additive) active ingredient A+B is:

[0143]

[0144] If the observed effect (O) is greater than the expected effect (E), then the combined effect is superadditive, i.e., synergistic. Mathematically, synergism corresponds to a positive value of the difference (OE). In the case of purely complementary addition of the active ingredient (expected activity), the difference (OE) is zero. A negative value of the difference (OE) indicates a loss of activity compared to the expected activity.

[0145] Example 1: Activity against Fusarium oxysporum (root rot)

[0146] The aim of this study was to evaluate the activity of a combination of phosphorus-based compounds and active ingredient B against Fusarium species, particularly Fusarium flavomarginata.

[0147] The following products were tested in laboratory experiments and are summarized in Tables 1a and 1b below:

[0148] - Phosphonic acid with the formula H3O3P (CAS No.: 13598-36-2).

[0149] - Potassium phosphite with the following formula: H2O3PK (CAS No.: 13977-65-6).

[0150] - Fludioxonil (CAS No.: 131341-86-1).

[0151] - Fluoxetine (CAS No.: 874967-67-6).

[0152] -Aranoic acid benzene-S-methyl (CAS No.: 135158-54-2).

[0153] - Fluopyram (CAS No.: 1352994-67-2).

[0154] - Chlorothalonil (CAS No.: 1417782-03-6).

[0155] - Fluopyram (CAS No.: 907204-31-3).

[0156] - Azoxystrobin (CAS No.: 131860-33-8).

[0157] - Fluopyram (CAS No.: 658066-35-4).

[0158] - Izoxystrobin (CAS No.: 125225-28-7).

[0159] - Tetrazazole (CAS No.: 1472649-01-6).

[0160] - Benzoflufenicol (CAS No.: 1072957-71-1).

[0161] - Fluopyram (CAS No.: 1228284-64-7).

[0162] - Trifluoropyridine (CAS No.: 1460292-16-3).

[0163] Fungal conidia from frozen storage were directly mixed into nutrient broth (PDB potato dextrose broth). A DMSO solution of the test compound was placed in a microtiter plate (96-well size), and nutrient broth containing fungal conidia was added. These test plates were incubated at 24°C for 48 hours, and the inhibition of growth was visually assessed.

[0164] Tables 1a and 1b show the in vitro efficacy (%) of the active ingredient against Fusarium oxysporum under controlled conditions.

[0165]

[0166] Table 1a

[0167]

[0168] Table 1b

[0169] The tested active ingredients according to the present invention, based on a phosphorus-based compound (active ingredient A) mixed with active ingredient B, guarantee good efficacy against Fusarium species, especially against Fusarium flavonoids.

[0170] Example 2: Activity against *Syndromea glabra* (leaf blight) of wheat

[0171] The aim of this study was to evaluate the activity of a combination of phosphorus-based compounds and active ingredient B against *Neurophila* species, particularly *Neurophila wheatii*.

[0172] The following products were tested in laboratory experiments and are summarized in Tables 2a and 2b below:

[0173] - Potassium phosphite with the following formula: H2O3PK (CAS No.: 13977-65-6).

[0174] - Diammonium phosphonate (or ammonium hydrogen phosphite) with the formula H9N2O3P (CAS No.: 51503-61-8).

[0175] - Dipotassium phosphate with the formula HO3PK2 (CAS No.: 13492-26-7).

[0176] - Phosphonic acid with the formula H3O3P (CAS No.: 13598-36-2).

[0177] - Fludioxonil (CAS No.: 131341-86-1).

[0178] - Fluoxetine (CAS No.: 874967-67-6).

[0179] - Prothioconazole (CAS No.: 178928-70-6).

[0180] - Stimazole (CAS No.: 131983-72-7).

[0181] -Aranoic acid benzene-S-methyl (CAS No.: 135158-54-2).

[0182] - Fluopyram (CAS No.: 907204-31-3).

[0183] - Fluopyram (CAS No.: 658066-35-4).

[0184] - Benzoflufenicol (CAS No.: 1072957-71-1).

[0185] Fungal conidia from frozen storage were directly mixed into nutrient broth (PDB potato dextrose broth). A DMSO solution of the test compound was placed in a microtiter plate (96-well size), and nutrient broth containing fungal conidia was added. These test plates were incubated at 24°C for 72 hours, and the inhibition of growth was visually determined.

[0186] Tables 2a and 2b show the in vitro efficacy (%) of the active ingredient against *Syngonium oryzae* under controlled conditions.

[0187]

[0188] Table 2a

[0189]

[0190] Table 2b

[0191] According to the present invention, the tested active ingredient based on a phosphorus-based compound (active ingredient A) mixed with active ingredient B ensures good efficacy against *Syngonium* species, especially against *Syngonium oryzae*.

[0192] Example 3: Activity against *Pyrrosia lingua* (rice blast fungus):

[0193] The aim of this study was to evaluate the activity of a combination of phosphorus-based compounds and active ingredient B against *Pyrhodotorula* species, particularly *Pyrhodotorula oryzae*.

[0194] The following products were tested in laboratory experiments and are summarized in Table 3 below:

[0195] - Phosphonic acid with the formula H3O3P (CAS No.: 13598-36-2).

[0196] - Fluorothiazol pyrone (CAS No.: 1003318-67-9).

[0197] - Fluopyram (CAS No.: 1228284-64-7).

[0198] - Trifluoropyridine (CAS No.: 1460292-16-3).

[0199] Fungal conidia from frozen storage were directly mixed into nutrient broth (potato sucrose broth). A DMSO solution of the test compound was placed in a microtiter plate (96-well size), and nutrient broth containing fungal conidia was added. These test plates were incubated at 24°C, and inhibition of pathogen growth was determined after 72 hours.

[0200] Table 3 shows the in vitro efficacy (%) of the active ingredient against *Pyrrosia lingua* under controlled conditions.

[0201]

[0202] Table 3

[0203] The tested active ingredients according to the present invention, based on a phosphorus-based compound (active ingredient A) mixed with active ingredient B, ensure good efficacy against pyriformes species, especially against *Pyrrosia lingua*.

Claims

1. A method for controlling, limiting, or preventing infection by plant pathogens of the Ascomycota phylum, the method comprising applying a pest-killing compound to the propagation material of the plant, the pest-killing compound comprising a phosphorus-based compound as active ingredient A and a compound selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fludioxonil, chlorfenapyr, prothioconazole, fluoxastrobin, cymoxanil, fluoxastrobin, fluoxastrobin, pyraclostrobin, difenoconazole, and fluoxastrobin. The active ingredient B of the following: pyraclostrobin, tebuconazole, tetrazolium, silthiamethoxam, dimethomorph, thiophanate-methyl, natamycin, benzo[a]fluopyram, fluopyram, triflupyridine, metalaxyl (metalaxyl-M), tetrazolium pyridaben, isoxaflutole, bromoxynil, thiamethoxam, imidacloprid, thiamethoxam, bromuconazole, chlorantraniliprole, tetrazolium pyridaben, fipronil, and any mixture thereof, wherein the application amount of said active ingredient B is 0.5 g to 500 g relative to 100 kg of plant propagation material.

2. The method according to claim 1, characterized in that... The active ingredient B is selected from aramid-type benzene-S-methyl, fludioxonil, fluopyram, fluinbamil, chlorfluazuron, prothioconazole, fluoxastrobin, cymoxanil, cymoxanil, fluoxastrobin, pyraclostrobin, fluopyram, styraclostrobin, tetrazolium, benzo[a]fluoroquinolones, fluopyram, triflupyridine, and any mixture thereof.

3. The method according to claim 1 or 2, characterized in that... The phosphorus-based compound is selected from phosphonic acids, one or more phosphonates, and any mixture thereof.

4. The method according to any one of the preceding claims, characterized in that... The plant pathogens described are selected from Fusarium diseases.

5. The method according to claim 4, characterized in that... The Fusarium disease mentioned is Fusarium chrysogenum.

6. The method according to any one of the preceding claims, characterized in that... The plant pathogens of the Ascomycota phylum are selected from diseases of the genus *Syngonium*.

7. The method according to claim 6, characterized in that... The disease caused by the genus *Syngonium* is *Syngonium leucosum*.

8. The method according to any one of the preceding claims, characterized in that... The plant pathogens of the Ascomycota phylum are selected from diseases of the genus Pyridia.

9. The method according to claim 8, characterized in that... The disease described is caused by *Pyreospora*.

10. The method according to any one of the preceding claims, characterized in that... The plants are selected from vegetables, legumes, oil crops, sugar beets, fiber plants, and grains.

11. The method according to any one of the preceding claims, characterized in that... The application amount can be 1 to 500 grams of the active ingredient A relative to 100 kg of plant propagation material, preferably 10 to 400 grams of the active ingredient A, preferably 20 to 350 grams of the active ingredient A, preferably 30 to 300 grams of the active ingredient A, and more preferably 30 to 200 grams of the active ingredient A.

12. The method according to any one of the preceding claims, characterized in that... The application amount is relative to 100 kg of plant propagation material, from 1 gram to 500 grams of the active ingredient B, preferably from 1 gram to 400 grams of the active ingredient B, preferably from 1 gram to 300 grams of the active ingredient B, preferably from 1 gram to 200 grams of the active ingredient B, preferably from 1 gram to 100 grams of the active ingredient B, preferably from 1 gram to 75 grams of the active ingredient B, preferably from 1 gram to 50 grams of the active ingredient B, and more preferably from 1 gram to 30 grams of the active ingredient B.

13. The method according to any one of the preceding claims, characterized in that... The weight ratio of active ingredient A to active ingredient B is 1:100 to 1000:1, preferably 1:100 to 400:1, more preferably 1:100 to 100:1, and even more preferably 1:10 to 10:

1.

14. The method according to any one of the preceding claims, characterized in that... The plant propagation material is plant seeds.

15. A plant propagation material treated with a pest control combination as defined in any of the preceding claims.

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