Molecular markers closely linked to pepper seed male fertility gene and application thereof

By providing molecular markers Ind146 and Ind147, which are closely linked to the male fertility gene in pepper kernels, the problems of slow speed and high cost of artificial emasculation in pepper seed production have been solved, realizing efficient, simple and high-purity pepper breeding.

CN122609741APending Publication Date: 2026-08-21INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI +1
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Patent Information

Application Number
CN202610901188.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-22
Publication Date
2026-08-21

AI Technical Summary

Technical Problem

In current chili seed production, artificial emasculation is slow, costly, and makes it difficult to guarantee the purity of hybrids. Research on nuclear male sterility genes started relatively late, and the molecular mechanisms are unclear, making it difficult to achieve efficient breeding.

Method used

Molecular markers Ind146 and Ind147, which are closely linked to the male fertility gene in pepper kernels, and their primer sets are provided for PCR amplification and detection of the male fertility genotype in pepper kernels. The auxiliary breeding methods include PCR amplification and genotype determination.

Benefits of technology

It has enabled precise localization of male fertility genes in chili pepper kernels and efficient breeding, which is simple and fast, improves the purity and yield of hybrids, and reduces seed production costs.

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Abstract

The application belongs to the field of plant genetic breeding, and discloses a molecular marker closely linked to a pepper nuclear male sterility gene and application thereof, and specifically discloses a molecular marker closely linked to a pepper nuclear male sterility gene, wherein the molecular marker comprises Ind146 and / or Ind147, the sequence of the Ind146 consists of the nucleotide sequence shown in SEQ ID NO:1 and SEQ ID NO:2, and the sequence of the Ind147 consists of the nucleotide sequence shown in SEQ ID NO:5 and SEQ ID NO:6. The molecular marker provided by the application realizes fine positioning of the pepper nuclear male sterility gene, and lays a foundation for final cloning of the pepper nuclear male sterility gene and establishment of a molecular marker assisted breeding system. The molecular marker of the application can be simply, rapidly and high-throughput applied to pepper seed production and breeding practice.
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