Isoelectric focusing capillary electrophoresis apparatus for antibody drug analysis

Abstract

The utility model discloses an isoelectric focusing capillary electrophoresis apparatus for antibody drug analysis, which belongs to the technical field of capillary electrophoresis apparatuses and comprises a shell, a high-voltage direct-current power supply, a capillary part and a fluorescence detector, the high-voltage direct-current power supply is mounted in the shell, and the capillary part is positioned in the shell and positioned at the upper part of the high-voltage direct-current power supply; the positive output end and the negative output end of the high-voltage direct-current power source are communicated with the two ends of the capillary tube part respectively, the fluorescence detector is located in the shell and located above the capillary tube part, a discharging box is arranged in the shell, a feeding pipe capable of achieving movable feeding is arranged in the shell, a feeding opening is formed in the feeding pipe, and a piston is arranged in the feeding pipe in a sliding mode; an extraction assembly for controlling the piston to move is arranged outside the feeding pipe, and a feeding assembly for moving the feeding pipe so as to feed the capillary tube part is arranged in the shell, so that the suction and release amount of the sample solution can be conveniently adjusted according to requirements, and the problem of insufficient sampling of the sample solution is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of capillary electrophoresis instruments, and more specifically to an isoelectric focusing capillary electrophoresis instrument for antibody drug analysis. Background Art

[0002] Capillary electrophoresis uses elastic quartz capillaries as separation channels and high-voltage DC electric fields as driving forces. It separates components in the sample based on differences in their mobility (migration speed under unit electric field strength) and distribution behavior.

[0003] Chinese patent application No. CN201922465738.6 discloses an ICE3 protein analysis isoelectric focusing capillary electrophoresis instrument, comprising a shell, a support plate is horizontally arranged on the inner side of the shell, a feed trough is installed on the upper surface of the support plate, a mounting plate is fixed to the upper surface of the support plate by bolts, a capillary is installed on the mounting plate, a sample trough is arranged on one side of the mounting plate, a buffer trough is arranged on the other side of the mounting plate, a movable plate is movably arranged in the horizontal direction on the inner side of the shell, a lifting cylinder is fixed on one side of the movable plate, a pipette mounting seat is fixed on the top of the lifting cylinder, and a plurality of sampling pipettes are installed on the bottom of the pipette mounting seat; the capillary electrophoresis instrument does not require manual addition of sample solution, is easy to operate, avoids waste of sample solution, and the mounting plate is easy to disassemble, so as to facilitate replacement of the capillary.

[0004] When the above scheme is in use, the sample solution is sucked and discharged through the sampling pipette, but the sampling pipette drives the pressure plate to move by the extrusion cylinder to achieve suction and discharge, wherein the extension length of the extrusion cylinder is fixed, resulting in the suction of the sampling pipette being fixed each time and cannot be changed in real time according to requirements; at the same time, the design of multiple sampling pipettes has high requirements on the horizontality of the material trough. When the material trough is not in a horizontal state, the sampling pipette at the end may not be able to continue to receive a sufficient amount of sample solution, affecting the measurement result. A solution to the above problems is proposed below. Utility Model Content

[0005] In view of the problems existing in the prior art, the purpose of the utility model is to provide an isoelectric focusing capillary electrophoresis instrument for antibody drug analysis, which can conveniently adjust the amount of sample solution absorption and release according to requirements and will not cause the problem of insufficient sampling of sample solution.

[0006] To solve the above problems, the utility model adopts the following technical solutions.

[0007] An isoelectric focusing capillary electrophoresis instrument for antibody drug analysis comprises a shell, a high-voltage DC power supply, a capillary portion and a fluorescence detector, wherein the high-voltage DC power supply is installed inside the shell, the capillary portion is located inside the shell and on the upper portion of the high-voltage DC power supply, the positive and negative output ends of the high-voltage DC power supply are respectively connected to the two ends of the capillary portion, the fluorescence detector is located inside the shell and above the capillary portion for detecting the capillary portion, a material discharge box is arranged inside the shell, a feeding tube for movable feeding is arranged inside the shell, a feeding port is opened on the feeding tube, a piston is slidably arranged inside the feeding tube, an extraction component for controlling the movement of the piston is arranged outside the feeding tube, and a feeding component for moving the feeding tube to load the capillary portion is arranged inside the shell.

[0008] Preferably, the extraction assembly includes a servo motor, a main gear, a slave gear, a first screw rod and a mounting plate, the mounting plate is installed at one end of the feeding tube, the servo motor is fixed on the mounting plate, the main gear and the servo motor are fixedly connected, the first screw rod is fixedly arranged at one end of the piston, the slave gear is sleeved on the first screw rod and threadedly matched, the slave gear and the mounting plate are rotationally matched, and the main gear and the slave gear are meshed with each other.

[0009] Preferably, the feeding assembly includes a second screw rod, a driving motor, a moving block and a cylinder. The second screw rod is rotatably arranged inside the outer frame. The driving motor is fixed on the outer frame. The output end of the driving motor is fixedly connected to one end of the second screw rod. The moving block is sleeved on the second screw rod and threadedly matched. The cylinder is fixed on the moving block. The output end of the cylinder is fixedly connected to the feeding pipe.

[0010] Preferably, the material discharging box is in the shape of an inverted cone.

[0011] Preferably, a support rod is provided on one side of the second screw rod, the support rod is fixedly connected to the housing, and the support rod passes through the moving block and is slidably fitted.

[0012] Preferably, a sealing cover is provided on the top of the shell.

[0013] Preferably, a controller is disposed inside the housing.

[0014] Compared with the prior art, the advantages of the present invention are:

[0015] 1. This solution can accurately control the rotation angle through the servo motor, thereby controlling the moving distance of the first screw rod, that is, the moving distance of the piston, and changing the amount of solution sample sucked and squeezed out according to different needs. A larger feeding tube is used to avoid the problem of insufficient extraction due to the unevenness of the discharge box. The inverted cone-shaped discharge box can avoid waste caused by incomplete extraction of the solution inside the discharge box. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 It is a cross-sectional structural schematic diagram of the front view of the utility model;

[0018] Figure 3 It is a cross-sectional structural schematic diagram of a side view of the utility model;

[0019] Figure 4 It is a schematic diagram of the rotation connection structure of the slave gear and the mounting plate of the utility model.

[0020] Description of the numbers in the figure:

[0021] 1. Housing; 2. High-voltage DC power supply; 3. Capillary part; 4. Fluorescence detector; 5. Discharge box; 6. Feeding tube; 7. Feeding port; 8. Piston; 9. Extraction assembly; 10. Feeding assembly; 11. Servo motor; 12. Main gear; 13. Slave gear; 14. First screw rod; 15. Mounting plate; 16. Second screw rod; 17. Drive motor; 18. Moving block; 19. Cylinder; 20. Support rod; 21. Sealing cover; 22. Controller. DETAILED DESCRIPTION

[0022] Embodiment 1:

[0023] See also Figure 1-4 , an antibody drug analysis isoelectric focusing capillary electrophoresis instrument, comprising a shell 1, a high-voltage DC power supply 2, a capillary portion 3 and a fluorescence detector 4, a controller 22 is fixedly arranged inside the shell 1, the high-voltage DC power supply 2 is fixed inside the shell 1 and is located on one side of the controller 22, the high-voltage DC power supply 2 and the controller 22 are electrically connected, the capillary portion 3 is fixed inside the shell 1 and is located on the upper part of the high-voltage DC power supply 2, the positive and negative output ends of the high-voltage DC power supply 2 are respectively connected to the two ends of the capillary portion 3, the fluorescence detector 4 is fixed inside the shell 1 and is located above the capillary portion 3 for detecting the capillary portion 3, the fluorescence detector 4 is electrically connected to the controller 22, and a sealing cover 21 that plays a dustproof sealing role is detachably arranged on the top of the shell 1.

[0024] A material discharging box 5 is arranged inside the shell 1, and the placement box and the shell 1 are detachably matched. The material discharging box 5 is in an inverted cone shape, and the material discharging box 5 is located on one side of the capillary portion 3. A feeding tube 6 for mobile feeding is arranged inside the shell 1. A feeding port 7 is opened on the feeding tube 6, and the feeding port 7 is in the shape of a needle tube. A piston 8 is slidably arranged inside the feeding tube 6. An extraction component 9 for controlling the movement of the piston 8 is arranged outside the feeding tube 6. The extraction component 9 includes a servo motor 11, a main gear 12, a slave gear 13, The first screw rod 14 and the mounting plate 15, the mounting plate 15 is installed at one end of the feeding tube 6, the servo motor 11 is fixed on the mounting plate 15, the servo motor 11 and the controller 22 are electrically connected, the main gear 12 and the servo motor 11 are fixedly connected, the first screw rod 14 is fixedly arranged at one end of the piston 8, the slave gear 13 is sleeved on the first screw rod 14 and threadedly matched, the slave gear 13 and the mounting plate 15 are rotationally matched, the main gear 12 and the slave gear 13 are meshed with each other, and the driving wheel and the driven wheel are exactly the same.

[0025] A feeding assembly 10 for moving the feeding tube 6 to realize feeding of the capillary part 3 is arranged inside the outer shell 1. The feeding assembly 10 includes a second screw rod 16, a driving motor 17, a moving block 18 and a cylinder 19. The second screw rod 16 is rotatably arranged inside the outer frame, the driving motor 17 is fixed on the outer frame, the output end of the driving motor 17 is fixedly connected to one end of the second screw rod 16, the moving block 18 is sleeved on the second screw rod 16 and threadedly matched, a support rod 20 is arranged on one side of the second screw rod 16, the support rod 20 is fixedly connected to the outer shell 1, the support rod 20 passes through the moving block 18 and is slidably matched, the cylinder 19 is fixed on the moving block 18, and the output end of the cylinder 19 is fixedly connected to the feeding tube 6.

[0026] Working principle:

[0027] When in use, open the sealing cover 21, take out the material discharging box 5, place the solution sample inside the material discharging box 5, and then put the material discharging box 5 back into the housing 1 and seal it with the sealing cover 21;

[0028] The controller 22 controls the driving motor 17 to start, and the driving motor 17 drives the second screw rod 16 to rotate, thereby driving the moving block 18 to move, so that the moving block 18 moves to the end of the second screw rod 16 close to the discharge box 5. At this time, the feeding tube 6 is located at the top of the discharge box 5, and the cylinder 19 is retracted. The cylinder 19 drives the feeding tube 6 to move downward, so that the feeding tube 6 drives the feeding port 7 to move downward and extend into the discharge box 5.

[0029] The controller 22 controls the servo motor 11 to start, the servo motor 11 drives the main gear 12 to rotate, the main gear 12 drives the slave gear 13 to rotate, and since the slave gear 13 and the first screw rod 14 are threadedly matched, the rotating slave gear 13 drives the first screw rod 14 to move, and the movement of the first screw rod 14 drives the piston 8 to move, thereby reducing the air pressure inside the feed pipe, and the solution sample inside the discharge box 5 is sucked in through the feed port 7 to complete the suction;

[0030] After the material is sucked, the control cylinder 19 is started, and then the driving motor 17 is controlled to rotate in the opposite direction, so that the second screw rod 16 is close to one end of the capillary part 3 when the moving card is moved. At this time, the feeding port 7 of the feeding tube 6 is located at the feeding end of the capillary;

[0031] The controller 22 controls the cylinder 19 to retract, and the cylinder 19 drives the feeding port 7 of the feeding tube 6 to extend into the feeding end of the capillary part 3. The controller 22 controls the servo motor 11 to rotate in the opposite direction, thereby driving the piston 8 to rotate in the opposite direction, pushing the solution sample into the capillary part 3. The controller 22 controls the high-voltage DC power supply 2 to supply power to the capillary part 3, and the fluorescence detector 4 is used for detection.

[0032] By changing the rotation angle of the servo motor 11, the extrusion amount each time can be changed to achieve precise control. The formula is as follows: extrusion amount = (rotation angle of the servo motor 11 / 360)*pitch of the first screw rod 14*bottom area of ​​the feeding tube 6.

Claims

1. An isoelectric focusing capillary electrophoresis instrument for antibody drug analysis, characterized in that: The invention comprises a housing (1), a high-voltage DC power supply (2), a capillary portion (3) and a fluorescence detector (4), wherein the high-voltage DC power supply (2) is installed inside the housing (1), the capillary portion (3) is located inside the housing (1) and above the high-voltage DC power supply (2), the positive and negative output ends of the high-voltage DC power supply (2) are respectively connected to the two ends of the capillary portion (3), and the fluorescence detector (4) is located inside the housing (1) and above the capillary portion (3) for detecting the fluorescence of the capillary portion (3). ), a material discharge box (5) is arranged inside the shell (1), a material feeding tube (6) for movable material feeding is arranged inside the shell (1), a material feed port (7) is provided on the material feeding tube (6), a piston (8) is slidably arranged inside the material feeding tube (6), an extraction component (9) for controlling the movement of the piston (8) is arranged outside the material feeding tube (6), and a material feeding component (10) for moving the material feeding tube (6) so as to realize material feeding to the capillary part (3) is arranged inside the shell (1).

2. The isoelectric focusing capillary electrophoresis instrument for antibody drug analysis according to claim 1, characterized in that: The extraction assembly (9) comprises a servo motor (11), a main gear (12), a slave gear (13), a first screw rod (14) and a mounting plate (15); the mounting plate (15) is mounted on one end of the feeding tube (6); the servo motor (11) is fixed on the mounting plate (15); the main gear (12) and the servo motor (11) are fixedly connected; the first screw rod (14) is fixedly arranged on one end of the piston (8); the slave gear (13) is sleeved on the first screw rod (14) and threadedly matched; the slave gear (13) and the mounting plate (15) are rotationally matched; the main gear (12) and the slave gear (13) are meshed with each other.

3. The isoelectric focusing capillary electrophoresis instrument for antibody drug analysis according to claim 1, characterized in that: The feeding assembly (10) comprises a second screw rod (16), a driving motor (17), a moving block (18) and a cylinder (19); the second screw rod (16) is rotatably arranged inside an outer frame; the driving motor (17) is fixed on the outer frame; the output end of the driving motor (17) is fixedly connected to one end of the second screw rod (16); the moving block (18) is sleeved on the second screw rod (16) and threadedly matched; the cylinder (19) is fixed on the moving block (18); and the output end of the cylinder (19) is fixedly connected to a feeding pipe (6).

4. The isoelectric focusing capillary electrophoresis instrument for antibody drug analysis according to claim 1, characterized in that: The material discharging box (5) is in the shape of an inverted cone.

5. The isoelectric focusing capillary electrophoresis instrument for antibody drug analysis according to claim 3, characterized in that: A support rod (20) is provided on one side of the second screw rod (16); the support rod (20) is fixedly connected to the housing (1); and the support rod (20) passes through the moving block (18) and is slidably fitted therewith.

6. The isoelectric focusing capillary electrophoresis instrument for antibody drug analysis according to claim 1, characterized in that: A sealing cover (21) is provided on the top of the housing (1).

7. The isoelectric focusing capillary electrophoresis instrument for antibody drug analysis according to claim 1, characterized in that: A controller (22) is arranged inside the housing (1).

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