Culture device capable of continuously harvesting hog cholera virus liquid

Abstract

The utility model discloses a culture device capable of continuously harvesting hog cholera virus liquid, which relates to the technical field of hog cholera virus liquid culture and comprises a base for improving the stability of the device, one side of the base is fixedly connected with a lead for supplying power to the base, and a mounting shell is inserted into the base. The bottom of an inner cavity of the mounting shell is fixedly connected with a driving motor, and the top of a rotating shaft of the driving motor is fixedly connected with a hog cholera virus liquid culture inner tank. A nutrient solution and hog cholera viruses are injected into the hog cholera virus liquid culture inner tank along the liquid adding pipe for virus culture, and when hog cholera virus liquid is collected, the control panel can control the output power of the driving motor, so that the output motor drives the inner tank to rotate at a high speed, and the hog cholera viruses are cultured in the hog cholera virus liquid culture inner tank. The classical swine fever virus liquid in the classical swine fever virus culture inner tank is centrifugally separated, and the settled classical swine fever virus liquid is filtered through the microporous filter element, so that the classical swine fever virus liquid culture device has an impurity removal effect and a virus liquid purification function.

Description

Technical Field

[0001] The utility model relates to the technical field of classical swine fever virus liquid culture, and particularly relates to a culture device capable of continuously harvesting classical swine fever virus liquid. Background Technique

[0002] Virus liquid culture is a method to obtain live virus, which is not only of extremely important value for the definite diagnosis of diseases, but also an indispensable prerequisite for carrying out subsequent research in virology, such as research on virus inheritance and variation, exploration of pathogenic mechanisms, detection of drug sensitivity, research and development of corresponding vaccines, etc.; virus culture has also been the basis for comparing and evaluating newly developed immunological methods and molecular virology methods; currently commonly used culture vessels include: culture dishes, multi-well culture plates, culture tubes, spinner flasks, etc.; culture dishes are rectangular, trapezoidal or circular, and are available in two textures, glass and plastic. The bottle body is transparent, and the wall thickness of the bottle is uniform and flat. When culturing, it is placed flat to allow cells to adhere and grow.

[0003] For example, when culturing classical swine fever virus, in the existing culture methods such as using culture dishes, culture tubes or spinner flasks, etc., there will be a large amount of impurities such as cell debris in the classical swine fever virus liquid after culture, and other impurity removal devices are still needed to remove the impurities in the classical swine fever virus liquid. However, the culture device itself does not have the function of removing impurities and purifying the classical swine fever virus liquid, resulting in the culture of classical swine fever virus liquid and causing pollution to other devices, and there is a certain inconvenience in use. Summary of the Utility Model

[0004] The utility model provides a culture device capable of continuously harvesting classical swine fever virus liquid to solve the problems raised in the above background technique.

[0005] To solve the above technical problems, the technical solution adopted by the utility model is:

[0006] A culture device capable of continuously harvesting classical swine fever virus liquid includes a base for improving the stability of the device. One side of the base is fixedly connected with a wire for supplying power to the base. An installation shell is inserted into the interior of the base. A driving motor is fixedly connected to the bottom of the inner cavity of the installation shell. The top of the rotating shaft of the driving motor is fixedly connected with an inner tank for culturing classical swine fever virus liquid.

[0007] The further improvement of the technical solution of the utility model lies in that: a control panel of the control device is fixedly connected to the surface of the installation shell, and an outer tank for preventing collision is fixedly connected to the top of the installation shell.

[0008] The further improvement of the technical solution of the utility model lies in that: a chute for limiting the inner tank for culturing classical swine fever virus liquid is fixedly connected to the inner wall of the outer tank, and a convex block arranged on the surface of the inner tank for culturing classical swine fever virus liquid is lapped on the inner wall of the chute.

[0009] A further improvement of the technical solution of the present utility model lies in that: a dust-proof tank cover is threadedly connected to the top of the outer tank, and a sealing rubber plug is inserted at the upper end of the inner tank for culturing classical swine fever virus liquid.

[0010] A further improvement of the technical solution of the present utility model lies in that: a liquid collection pipe leading to the bottom of the inner cavity of the inner tank for culturing classical swine fever virus liquid is inserted into the sealing rubber plug, and one end of the liquid collection pipe is fixedly connected with a microporous filter element for filtering and removing cell debris.

[0011] A further improvement of the technical solution of the present utility model lies in that: a liquid addition pipe leading to the middle of the inner cavity of the inner tank for culturing classical swine fever virus liquid is arranged inside the sealing rubber plug, and an air vent pipe is arranged inside the sealing rubber plug.

[0012] A further improvement of the technical solution of the present utility model lies in that: the mouth of the inner tank for culturing classical swine fever virus liquid turns outwards and wraps the mouth of the outer tank.

[0013] Due to the adoption of the above technical solution, the technical progress achieved by the present utility model compared with the prior art is:

[0014] The present utility model provides a culture device for continuously harvesting classical swine fever virus liquid. By injecting nutrient solution and classical swine fever virus into the inner tank for culturing classical swine fever virus liquid along the liquid addition pipe to culture the virus, when harvesting the classical swine fever virus liquid, the control panel can control the output power of the driving motor, so that the output motor drives the inner tank to rotate at a high speed, centrifugally separates the classical swine fever virus liquid in the inner tank for culturing classical swine fever virus, and filters the settled classical swine fever virus liquid through the microporous filter element, so that the culture device for classical swine fever virus liquid has the effects of removing impurities and purifying the purity of the virus liquid, making it more convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is the front view structural schematic diagram of the present utility model;

[0016] Figure 2 is the separated state structural schematic diagram of the present utility model;

[0017] Figure 3 is the exploded structural schematic diagram of the present utility model;

[0018] Figure 4 is the sectional view structural schematic diagram of the present utility model.

[0019] In the figure: 1, base; 2, wire; 3, mounting shell; 4, control panel; 5, outer tank; 6, tank cover; 7, driving motor; 8, inner tank for culturing classical swine fever virus liquid; 9, convex block; 10, sliding groove; 11, sealing rubber plug; 12, liquid collection pipe; 13, microporous filter element; 14, liquid addition pipe; 15, air vent pipe. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0020] The present utility model will be further described in detail below in conjunction with embodiments:

[0021] Embodiment 1

[0022] As Figures 1-4 shown, the present utility model provides a culture device for continuously harvesting classical swine fever virus liquid, including a base 1 for improving the stability of the device. One side of the base 1 is fixedly connected with a wire 2 for supplying power to the base 1. An installation shell 3 is inserted into the interior of the base 1. A driving motor 7 is fixedly connected to the bottom of the inner cavity of the installation shell 3. The top of the rotating shaft of the driving motor 7 is fixedly connected with an inner tank 8 for culturing classical swine fever virus liquid.

[0023] In this embodiment, the driving motor 7 is started through the control panel 4, so that the inner tank 8 for culturing classical swine fever virus liquid rotates at a high speed in the outer tank 5, centrifugally separating the virus and cell debris. Subsequently, after a short sedimentation, the classical swine fever virus liquid and impurities are stratified, and the stratified classical swine fever virus liquid is collected through the liquid collection pipe 12.

[0024] Embodiment 2

[0025] As Figures 1-4 shown, on the basis of Embodiment 1, the present utility model provides a technical solution: Preferably, it includes a base 1 for improving the stability of the device, characterized in that: one side of the base 1 is fixedly connected with a wire 2 for supplying power to the base 1. An installation shell 3 is inserted into the interior of the base 1. A driving motor 7 is fixedly connected to the bottom of the inner cavity of the installation shell 3. The top of the rotating shaft of the driving motor 7 is fixedly connected with an inner tank 8 for culturing classical swine fever virus liquid. The control panel 4 of the control device is fixedly connected to the surface of the installation shell 3. An outer tank 5 for preventing bumps is fixedly connected to the top of the installation shell 3. A sliding groove 10 for limiting the inner tank 8 for culturing classical swine fever virus liquid is fixedly connected to the inner wall of the outer tank 5. A convex block 9 arranged on the surface of the inner tank 8 for culturing classical swine fever virus liquid is lapped on the inner wall of the sliding groove 10.

[0026] In this embodiment, when culturing classical swine fever virus liquid, first, the classical swine fever virus and nutrient solution are injected into the inner tank 8 for culturing classical swine fever virus liquid along the liquid adding pipe 14, and the device is placed in a suitable temperature and humidity environment, so that the classical swine fever virus is cultured in the inner tank 8 for culturing classical swine fever virus liquid. When collecting the classical swine fever culture solution, the outer tank 5 is placed into the base 1, the driving motor 7 is started through the control panel 4, so that the inner tank 8 for culturing classical swine fever virus liquid rotates at a high speed in the outer tank 5, centrifugally separating the virus and cell debris. Subsequently, after a short sedimentation, the classical swine fever virus liquid and impurities are stratified, and the stratified classical swine fever virus liquid is collected through the liquid collection pipe 12

[0027] Embodiment 3

[0028] As Figures 1-4As shown, on the basis of Embodiment 1, the present utility model provides a technical solution: Preferably, it includes a base 1 for improving the stability of the device, characterized in that: One side of the base 1 is fixedly connected with a wire 2 for supplying power to the base 1. An installation shell 3 is inserted into the interior of the base 1. At the bottom of the inner cavity of the installation shell 3, a driving motor 7 is fixedly connected. At the top of the rotating shaft of the driving motor 7, an inner tank 8 for culturing swine fever virus liquid is fixedly connected. The top of the outer tank 5 is threadedly connected with a tank cover 6 for preventing dust from falling. The upper end of the inner tank 8 for culturing swine fever virus liquid is inserted with a sealing rubber plug 11. Inside the sealing rubber plug 11, a liquid collection pipe 12 leading to the bottom of the inner cavity of the inner tank 8 for culturing swine fever virus liquid is inserted. One end of the liquid collection pipe 12 is fixedly connected with a microporous filter element 13 for filtering out cell debris. Inside the sealing rubber plug 11, a liquid addition pipe 14 leading to the middle of the inner cavity of the inner tank 8 for culturing swine fever virus liquid is arranged. Inside the sealing rubber plug 11, a ventilation pipe 15 is arranged. The mouth of the inner tank 8 for culturing swine fever virus liquid turns outwards and wraps the mouth of the outer tank 5.

[0029] In this embodiment, the driving motor 7 is started through the control panel 4, so that the inner tank 8 for culturing swine fever virus liquid rotates at a high speed inside the outer tank 5, centrifugally separating the virus and cell debris. Subsequently, after a short settlement, the swine fever virus liquid and impurities are stratified. The stratified swine fever virus liquid is collected through the liquid collection pipe 12. During the collection process, the swine fever virus liquid is filtered through the microporous filter element 13, making the purity of the swine fever virus liquid higher and making it more convenient to use. At the same time, the setting of the liquid addition pipe 14 can also supplement fresh maintenance liquid after liquid collection, enabling continuous culturing and continuous collection of the virus.

[0030] Next, specifically describe the working principle of the culturing device for continuously harvesting swine fever virus liquid.

[0031] As Figures 1-4 shown, when culturing swine fever virus liquid, first inject swine fever virus and nutrient solution into the inner tank 8 for culturing swine fever virus liquid along the liquid addition pipe 14, and make the device in a suitable temperature and humidity environment, so that the swine fever virus is cultured in the inner tank 8 for culturing swine fever virus liquid. When collecting the swine fever culture solution, place the outer tank 5 into the base 1, start the driving motor 7 through the control panel 4, make the inner tank 8 for culturing swine fever virus liquid rotate at a high speed inside the outer tank 5, centrifugally separate the virus and cell debris. Subsequently, after a short settlement, the swine fever virus liquid and impurities are stratified. The stratified swine fever virus liquid is collected through the liquid collection pipe 12, and during the collection process, the swine fever virus liquid is filtered through the microporous filter element 13, making the purity of the swine fever virus liquid higher and making it more convenient to use. At the same time, the setting of the liquid addition pipe 14 can also supplement fresh maintenance liquid after liquid collection, enabling continuous culturing and continuous collection of the virus.

[0032] The above has generally described the present utility model in detail. However, based on the present utility model, some modifications or improvements can be made, which are obvious to those of ordinary skill in the art. Therefore, modifications or improvements that do not depart from the spirit and idea of the present utility model are within the protection scope of the present utility model.

Claims

1. A culture device for continuously harvesting swine fever virus liquid, comprising a base (1) for improving the stability of the device, characterized in that: A wire (2) for supplying power to the base (1) is fixedly connected to one side of the base (1); a mounting shell (3) is plugged into the interior of the base (1); a driving motor (7) is fixedly connected to the bottom of the inner cavity of the mounting shell (3); and a swine fever virus liquid culture inner tank (8) is fixedly connected to the top of the rotating shaft of the driving motor (7).

2. The culture device for continuously harvesting classical swine fever virus liquid according to claim 1, characterized in that: A control panel (4) of a control device is fixedly connected to the surface of the installation shell (3), and an anti-collision outer tank (5) is fixedly connected to the top of the installation shell (3).

3. A culture device for continuously harvesting classical swine fever virus liquid according to claim 2, characterized in that: The inner wall of the outer tank (5) is fixedly connected with a slide groove (10) for limiting the position of the swine fever virus liquid culture inner tank (8), and the inner wall of the slide groove (10) is overlapped with a protrusion (9) arranged on the surface of the swine fever virus liquid culture inner tank (8).

4. The culture device for continuously harvesting classical swine fever virus liquid according to claim 2, characterized in that: The top of the outer tank (5) is threadedly connected with a tank cover (6) for preventing dust from falling, and the upper end of the inner tank (8) for culturing swine fever virus liquid is plugged with a sealing rubber plug (11).

5. The culture device for continuously harvesting classical swine fever virus liquid according to claim 4, characterized in that: The sealing rubber plug (11) is internally plugged with a liquid collecting tube (12) leading to the bottom of the inner cavity of the swine fever virus liquid culture tank (8), and one end of the liquid collecting tube (12) is fixedly connected with a microporous filter element (13) for filtering and removing cell debris.

6. The culture device for continuously harvesting classical swine fever virus liquid according to claim 4, characterized in that: The sealing rubber stopper (11) is provided with a liquid adding pipe (14) leading to the middle of the inner cavity of the swine fever virus liquid culture inner tank (8), and the sealing rubber stopper (11) is provided with a ventilation pipe (15).

7. The culture device for continuously harvesting classical swine fever virus liquid according to claim 1, characterized in that: The mouth of the inner tank (8) for culturing the swine fever virus liquid is turned outward and wraps the mouth of the outer tank (5).

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