Rh typing kit based on solid phase agglutination method

Abstract

The utility model discloses an Rh typing kit based on a solid-phase agglutination method, which comprises a support seat, the middle part of which is configured to be of a hollow structure; the reaction plate is placed in the supporting seat and has a porous structure; the box cover covers the reaction plate, and the edge of the box cover is lapped on the supporting seat; the auxiliary device is mounted at the bottom of the supporting seat, is configured to be of a detachable structure and can heat or cool the reaction plate. The Rh typing kit based on the solid-phase agglutination method disclosed by the utility model is simple in structure, the heating base or the heat dissipation base can be quickly mounted, dismounted and switched by arranging the supporting seat and the auxiliary device, convenience is provided for the detection process, a large number of samples can be simultaneously detected, and the detection efficiency is improved.

Description

Technical Field

[0001] This invention relates to the field of biological detection technology, and in particular to an Rh typing kit based on solid-phase agglutination. Background Technology

[0002] Commonly used clinical methods for Rh genotyping (C, c, E, e) include: test tube method, microplate method, paper plate method, and microcolumn gel method. Different methods often require different testing tools (reagent kits).

[0003] During the testing process using the kit, it is often necessary to heat or cool the reaction plate. However, current detection kits based on solid-phase agglutination cannot heat or cool the reaction plate, requiring the assistance of heating or cooling equipment, which is time-consuming and labor-intensive.

[0004] Based on the above-mentioned technical problems, those skilled in the art urgently need to develop an Rh typing kit based on solid-phase agglutination that has a simple structure, can provide heating or cooling for the reaction plate, and can meet the requirements of repeated detection or multi-sample detection. Utility Model Content

[0005] The purpose of this invention is to provide an Rh typing kit based on solid-phase agglutination that has a simple structure, can provide heating or cooling for the reaction plate, and can meet the requirements of repeated detection or multi-sample detection.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] This invention relates to an Rh typing kit based on solid-phase agglutination, the kit comprising:

[0008] The support base, the middle of which is configured as a hollow structure; and

[0009] A reaction plate placed in the support base, the reaction plate having a porous structure;

[0010] A lid is placed over the reaction plate, with its edge overlapping the support base;

[0011] The kit also includes:

[0012] An auxiliary device installed at the bottom of the support base is configured as a detachable structure and is capable of providing heating or cooling to the reaction plate.

[0013] Furthermore, the support base has a ring of pads along its inner wall, and the reaction plate is placed on the pads, forming a clearance fit with the inner wall of the support base.

[0014] Preferably, the reaction plate is provided with reaction holes, which are arranged in twelve rows, with eight holes in each row.

[0015] Furthermore, the auxiliary device is a heating base or a heat dissipation base, both of which have a base structure and four positioning grooves on the upper edge of the base for connecting the support base.

[0016] Furthermore, the heating base is constructed by mounting a heating plate on the base.

[0017] Furthermore, the heat dissipation base is constructed by mounting a cooling fan on the base.

[0018] Preferably, the lid is made of transparent glass and has a handle on top.

[0019] Furthermore, the bottom of the support base is provided with four positioning pins that are adapted to the positioning groove.

[0020] In the above technical solution, the Rh typing kit based on solid-phase agglutination method provided by this utility model has the following beneficial effects:

[0021] The Rh typing reagent kit based on solid-phase agglutination method of this invention has a simple structure. By setting a support base and auxiliary devices, it is possible to quickly install and disassemble the heating base or the heat dissipation base, which provides convenience for the detection process. It can detect a large number of samples at the same time and improve the detection efficiency. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0023] Figure 1 A schematic diagram of the disassembly structure of an Rh typing kit based on solid-phase agglutination method provided for an embodiment of this utility model;

[0024] Figure 2 A top view of the reaction plate in an Rh typing kit based on solid-phase agglutination method provided for an embodiment of this utility model;

[0025] Figure 3 A schematic diagram of a heating base in an Rh typing kit based on solid-phase agglutination method provided for an embodiment of this utility model;

[0026] Figure 4 A schematic diagram of a heat dissipation base in an Rh typing kit based on solid-phase agglutination method provided for an embodiment of this utility model;

[0027] Figure 5 This is a schematic diagram of the detection arrangement of an Rh typing kit based on solid-phase agglutination method provided in an embodiment of this utility model.

[0028] Explanation of reference numerals in the attached figures:

[0029] 1. Support base; 2. Reaction plate; 3. Box cover; 4. Auxiliary devices;

[0030] 101. Pad; 102. Locating pin;

[0031] 201. Reaction well;

[0032] 401. Positioning groove;

[0033] 41. Heating base; 42. Heat dissipation base; 411. Heating plate; 421. Cooling fan. Detailed Implementation

[0034] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0035] See Figures 1-5 As shown;

[0036] This invention relates to an Rh typing kit based on solid-phase agglutination, the kit comprising:

[0037] Support 1, the middle part of which is configured as a hollow structure; and

[0038] The reaction plate 2 is placed in the support 1, and the reaction plate 2 has a porous structure;

[0039] The lid 3 covers the reaction plate 2, and its edge overlaps the support base 1;

[0040] The kit also includes:

[0041] An auxiliary device 4 is installed at the bottom of the support base 1. The auxiliary device 4 is configured as a detachable structure and is capable of providing heating or cooling to the reaction plate 2.

[0042] As a further description of this embodiment, the support base 1 is provided with a ring of pads 101 along its inner wall, and the reaction plate 2 is placed on the pads 101 and forms a clearance fit with the inner wall of the support base 1.

[0043] As a preferred technical solution in this embodiment, the reaction plate 2 is provided with reaction holes 201, which are arranged in twelve rows, with eight holes in each row.

[0044] As a further description of this embodiment, the auxiliary device 4 is a heating base 41 or a heat dissipation base 42, both of which have a base structure and four positioning grooves 401 are opened at the upper edge of the base for connecting the support base 1.

[0045] As a further description of this embodiment, the heating base 41 is constructed by mounting a heating plate 411 on the base.

[0046] As a further description of this embodiment, the heat dissipation base 42 is constructed by mounting a heat dissipation fan 421 on the base.

[0047] As a preferred technical solution in this embodiment, the box cover 3 is made of transparent glass and has a handle installed on the top.

[0048] As a further description of this embodiment, the bottom of the support base 1 is provided with four positioning pins 102 that are adapted to the positioning groove 401. In this embodiment, the auxiliary device 4 is configured as a switchable structure. Specifically, the heating plate 411 adopts the high-purity graphite heating plate of the EG series in the prior art and is installed in the base; the cooling fan 421 consists of two cooling fans of model DF1238B24H. When heating or cooling is required, the heating base 41 or the cooling base 42 can be switched by removing the one to be replaced from the positioning groove 401.

[0049] This invention provides an Rh typing kit based on solid-phase agglutination, the detection method of which is as follows:

[0050] Rh typing reagent (C, c, E, e) monoclonal antibodies were coated in reaction plate 2 and dried at 37°C for 24 hours. When using, Rh diluent was added first, followed by red blood cells. If the red blood cells contained Rh antigen, the antigen would bind to the antibody in reaction well 201. After centrifugation, positive reaction red blood cells would aggregate at the center of the bottom of reaction well 201 under the action of centrifugal force, while negative reaction red blood cells would spread out on the bottom surface of reaction well 201.

[0051] Solid-phase agglutination method for detecting Rh typing (C, c, E, e) has the advantages of excellent specificity, sensitivity, quality stability, short detection time, simple and easy method, easy result interpretation, simultaneous detection of a large number of samples, and easy automation.

[0052] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. An Rh typing kit based on solid-phase agglutination, characterized in that, The kit includes: Support base (1), the middle part of which is configured as a hollow structure; and A reaction plate (2) is placed in the support (1), and the reaction plate (2) has a porous structure; A lid (3) is placed on the reaction plate (2) and its edge overlaps the support base (1); The kit also includes: An auxiliary device (4) is installed at the bottom of the support base (1). The auxiliary device (4) is configured as a detachable structure and is capable of providing heating or cooling to the reaction plate (2).

2. The Rh typing kit based on solid-phase agglutination as described in claim 1, characterized in that, The support base (1) has a ring of pads (101) along its inner wall. The reaction plate (2) is placed on the pads (101) and forms a clearance fit with the inner wall of the support base (1).

3. The Rh typing kit based on solid-phase agglutination as described in claim 1, characterized in that, The reaction plate (2) is provided with reaction holes (201), and the reaction holes (201) are arranged in twelve rows, with eight holes in each row.

4. The Rh typing kit based on solid-phase agglutination as described in claim 1, characterized in that, The auxiliary device (4) is either a heating base (41) or a heat dissipation base (42), both of which have a base structure and four positioning grooves (401) on the upper edge of the base for connecting the support base (1).

5. The Rh typing kit based on solid-phase agglutination as described in claim 4, characterized in that, The heating base (41) is constructed by mounting a heating plate (411) on the base.

6. The Rh typing kit based on solid-phase agglutination as described in claim 4, characterized in that, The heat dissipation base (42) is constructed by mounting a cooling fan (421) on the base.

7. An Rh typing kit based on solid-phase agglutination according to any one of claims 1 to 6, characterized in that, The lid (3) is made of transparent glass and has a handle on top.

8. The Rh typing kit based on solid-phase agglutination as described in claim 4, characterized in that, The bottom of the support base (1) is provided with four positioning pins (102) that are adapted to the positioning groove (401).

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