A gas concentration control device

Abstract

The application provides a gas concentration control device, belonging to the field of full-automatic microbial adaptive evolution instrument gas concentration control, to solve the problem that the full-automatic microbial adaptive evolution instrument cannot control the gas concentration, comprising a circuit control board and a bottom plate, a storage battery, a gas pump and a circulating pump are connected to the circuit control board, a first electromagnetic valve is connected to the output end of the gas pump through a silica gel pipe, the output end of the first electromagnetic valve is connected to a first three-way pipe through a PU pipe, and the first three-way pipe is connected to a second three-way pipe through a PU pipe. The application is provided with a circulating pump; the gas circulating in the culture bin can be circulated through the gas circulating pipeline, the gas concentration in the culture bin can be detected in combination with a carbon dioxide sensor and an oxygen sensor, when the fluctuation range of the set gas concentration is exceeded, the automatic control is realized through the circuit control board, and the gas concentration is adjusted, so that the stable culture of small system microorganisms is realized.

Description

Technical Field

[0001] This utility model relates to the field of gas concentration control in fully automated microbial adaptive evolution instruments, and more specifically, to a gas concentration control device. Background Technology

[0002] Adaptive laboratory evolution is a method that artificially simulates the variation and selection processes in natural evolution under laboratory conditions. It leverages artificial selection pressure to achieve directed evolution of microorganisms and selects individuals with superior traits from the evolving population. Currently, it is commonly used to screen industrial production strains with specific phenotypes, good production performance, and good genetic stability.

[0003] Existing microbial adaptive evolution instruments have some problems in actual operation. For example, a microbial adaptive evolution instrument with publication number CN219136798U directly introduces compressed air into the culture system through an oxygen partial pressure controller when culturing small-scale microorganisms. After obtaining the maximum dissolved oxygen calibration, it controls the dissolved oxygen level inside the liquid, but it cannot control the gas concentration. Furthermore, it is known from actual practice that introducing compressed gas into the culture system will generate a large number of bubbles, and the bursting of bubbles will also generate a large shear force, which can easily have an adverse effect on the growth of microorganisms. Therefore, we have made improvements and proposed a gas concentration control device. Utility Model Content

[0004] The purpose of this invention is to address the problem that existing microbial adaptive evolution instruments cannot control gas concentration when culturing small-scale microbial systems.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A gas concentration control device to improve the above-mentioned problems.

[0007] The application is as follows:

[0008] The system includes a circuit control board and a base plate. The circuit control board is connected to a battery, an air pump, and a circulation pump. The output of the air pump is connected to a first solenoid valve via a silicone tube. The output of the first solenoid valve is connected to a first tee via a PU tube. The first tee is connected to a second tee via a PU tube. The second tee is connected to a third tee via a silicone tube. The second tee is connected to a proportional valve via a PU tube. The input of the proportional valve is connected to a second solenoid valve via a PU tube. The input of the second solenoid valve is connected to an external connecting pipe via a PU tube. A sealing cover is mounted on the base plate. A connecting plate is mounted on top of the sealing cover. A support base is mounted and fixed on the base plate. An mounting base is fixedly connected to the top of the support base. A culture chamber is fixedly mounted on top of the mounting base. An electric heater, an electric fan, and a temperature sensor are fixedly mounted inside the mounting base.

[0009] As a preferred technical solution of this application, the input end of the circulation pump is connected to a third solenoid valve through a silicone tube, the input end of the third solenoid valve is connected to a third three-way pipe through a silicone tube, and the output end of the circulation pump is connected to a carbon dioxide sensor through a silicone tube.

[0010] As a preferred technical solution of this application, the output end of the carbon dioxide sensor is connected to an oxygen sensor through a PU tube, the output end of the oxygen sensor is connected to a fourth solenoid valve through a PU tube, and the output end of the fourth solenoid valve is connected to a first three-way pipe through a PU tube.

[0011] As a preferred technical solution of this application, the third tee pipe is connected to the connecting plate through a silicone tube.

[0012] As a preferred technical solution of this application, the inner diameter of the sealing cover is larger than the diameter of the culture chamber, and the mounting base is fixed at the bottom center of the culture chamber.

[0013] As a preferred technical solution of this application, the electric fan is located in the center of the mounting base, and the support bases are symmetrically distributed on both sides of the bottom of the mounting base.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0015] In the scheme of this application:

[0016] 1. Equipped with a circulation pump; the circulation pump, third solenoid valve, carbon dioxide sensor, oxygen sensor, and fourth solenoid valve can form a gas circulation pipeline. During the operation of the culture chamber, the gas delivered to the culture chamber can be circulated through the gas circulation pipeline. Combined with the carbon dioxide sensor and oxygen sensor, the gas concentration in the culture chamber can be detected. When the gas concentration exceeds the set fluctuation range, the circuit control board will automatically control and adjust the gas concentration to achieve stable culture of small-scale microorganisms.

[0017] 2. It is equipped with a culture chamber; the circulating gas can be heated by an electric heater, and the gas and temperature inside the chamber can be mixed by the continuous drive of an electric fan, so as to ensure uniform temperature and gas concentration throughout the culture chamber. The gas temperature can be monitored in real time by a temperature sensor. Attached Figure Description

[0018] Figure 1 A three-dimensional structural diagram of the culture chamber for the gas concentration control device provided in this application;

[0019] Figure 2 A schematic diagram of the main cross-sectional structure of the culture chamber for the gas concentration control device provided in this application;

[0020] Figure 3 A top view of the culture chamber structure of the gas concentration control device provided in this application.

[0021] Figure 4 A schematic diagram of the piping arrangement for the gas concentration control device provided in this application;

[0022] Figure 5 This is a schematic diagram of the piping for the gas concentration control device provided in this application.

[0023] The diagram shows: 1. Circuit control board; 2. Battery; 3. Air pump; 4. First solenoid valve; 5. First tee pipe; 6. Second tee pipe; 7. Third tee pipe; 8. Proportional valve; 9. Second solenoid valve; 10. External pipe; 11. Circulation pump; 12. Third solenoid valve; 13. Carbon dioxide sensor; 14. Oxygen sensor; 15. Fourth solenoid valve; 16. Base plate; 17. Sealing cover; 18. Connecting plate; 19. Support base; 20. Mounting base; 21. Culture chamber; 22. Electric heater; 23. Electric fan; 24. Temperature sensor. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model.

[0025] Therefore, the following detailed description of the embodiments of this utility model is not intended to limit the scope of the claimed utility model, but merely to illustrate some embodiments of the utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without inventive effort are within the scope of protection of this utility model.

[0026] It should be noted that, unless otherwise specified, the embodiments and features and technical solutions in the present invention can be combined with each other.

[0027] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0028] In the description of this utility model, it should be noted that the terms "upper," "lower," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use, or the orientation or positional relationship commonly understood by those skilled in the art. These terms are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this utility model. In addition, the terms "first," "second," etc., are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0029] Example 1:

[0030] like Figures 1-5As shown, this embodiment proposes a gas concentration control device, including a circuit control board 1 and a base plate 16. A battery 2, an air pump 3, and a circulation pump 11 are connected to the circuit control board 1. The output end of the air pump 3 is connected to a first solenoid valve 4 via a silicone tube. The output end of the first solenoid valve 4 is connected to a first three-way valve 5 via a PU tube. The first three-way valve 5 is connected to a second three-way valve 6 via a PU tube. The second three-way valve 6 is connected to a third three-way valve 7 via a silicone tube. The second three-way valve 6 is connected to a proportional valve 8 via a PU tube. The input end of the proportional valve 8 is connected to a second solenoid valve 9 via a PU tube. The second solenoid valve 9... The input end is connected to an external tube 10 via a PU tube. A sealing cover 17 is installed on the base plate 16. A connecting plate 18 is installed on the top of the sealing cover 17. A support base 19 is installed and fixed on the base plate 16. An installation base 20 is fixedly connected to the top of the support base 19. A culture chamber 21 is installed and fixed on the top of the installation base 20. An electric heater 22, an electric fan 23, and a temperature sensor 24 are installed and fixed inside the installation base 20. The first solenoid valve 4, the proportional valve 8, the second solenoid valve 9, the third solenoid valve 12, the carbon dioxide sensor 13, the oxygen sensor 14, and the fourth solenoid valve 15 are all connected to the circuit control board 1.

[0031] Example 2:

[0032] The solution in Example 1 will be further described below with reference to its specific working method.

[0033] like Figure 1 and Figure 2 As shown, in a preferred embodiment, based on the above method, the input end of the circulation pump 11 is further connected to a third solenoid valve 12 via a silicone tube. The input end of the third solenoid valve 12 is connected to a third three-way pipe 7 via a silicone tube. The output end of the circulation pump 11 is connected to a carbon dioxide sensor 13 via a silicone tube. The output end of the carbon dioxide sensor 13 is connected to an oxygen sensor 14 via a PU tube. The output end of the oxygen sensor 14 is connected to a fourth solenoid valve 15 via a PU tube. The output end of the fourth solenoid valve 15 is connected to a first three-way pipe 5 via a PU tube. The circulation pump 11, the third solenoid valve 12, the carbon dioxide sensor 13, the oxygen sensor 14, and the fourth solenoid valve 15 can form a gas circulation pipeline. During the operation of the culture chamber 21, the gas delivered to the culture chamber 21 can be circulated through the gas circulation pipeline. Combined with the carbon dioxide sensor 13 and the oxygen sensor 14, the gas concentration in the culture chamber 21 can be detected. When the gas concentration exceeds the set fluctuation range, the circuit control board 1 automatically controls and adjusts the gas concentration to achieve stable culture of small-system microorganisms.

[0034] like Figure 1 and Figure 2As shown, in a preferred embodiment, based on the above method, the third three-way pipe 7 is further connected to the connecting plate 18 through a silicone tube, which can ensure that the gas can be stably replenished into the culture chamber 21.

[0035] like Figures 3-5 As shown, in a preferred embodiment, based on the above method, the inner diameter of the sealing cover 17 is larger than the diameter of the culture chamber 21. The mounting base 20 is fixed at the bottom center of the culture chamber 21. The electric fan 23 is located at the center inside the mounting base 20. The support bases 19 are symmetrically distributed on both sides of the bottom of the mounting base 20. The circulating gas can be heated by the electric heater 22. Combined with the continuous drive of the electric fan 23, the gas and temperature inside the chamber can be mixed evenly, ensuring uniform temperature and gas concentration throughout the culture chamber 21. The gas temperature can be monitored in real time by the temperature sensor 24.

[0036] Specifically, when this gas concentration control device is in use: First, the circulation pump 11, the third solenoid valve 12, the carbon dioxide sensor 13, the oxygen sensor 14, and the fourth solenoid valve 15 form a gas circulation pipeline, and the gas pump 3, the first solenoid valve 4, the proportional valve 8, the second solenoid valve 9, the circulation pump 11, the third solenoid valve 12, the carbon dioxide sensor 13, the oxygen sensor 14, and the fourth solenoid valve 15 in the pipeline are controlled by the circuit control board 1. When the device starts working, nitrogen, oxygen, carbon dioxide, etc. are connected to the external cylinder through the external pipe 10 as high-purity gas replenishment.

[0037] The proportional valve 8 connected to the external pipe 10 supplies gas into the culture chamber 21 through the second three-way pipe 6 and the third three-way pipe 7 connected by the pipeline. At the same time, the air pump 3 performs air suction, and the air pump 3 supplies air into the culture chamber 21 through the first solenoid valve 4, the first three-way pipe 5, the second three-way pipe 6 and the third three-way pipe 7 connected by the pipeline.

[0038] Meanwhile, during gas control, the circulating pump 11, connected to the third solenoid valve 12 and the third three-way pipe 7 via pipelines, can draw in the gas added to the culture chamber 21 and deliver it to the carbon dioxide sensor 13 and the oxygen sensor 14 via pipelines. Subsequently, the gas circulates through the fourth solenoid valve 15, the first three-way pipe 5, the second three-way pipe 6, and the third three-way pipe 7. During the gas circulation process, the gas concentration can be detected. If the gas concentration exceeds the set fluctuation range, the proportional valve 8 and the second solenoid valve 9 are controlled by the circuit control board 1 to adjust the gas replenishment, thereby regulating the gas concentration.

[0039] Furthermore, combined with the culture consumables of the fully automated microbial adaptive evolutionary apparatus (EVOL cell), the highly permeable material of the culture tubing allows for gas exchange between the culture liquid and the environment of the culture chamber 21, thereby achieving control of the gas concentration in the liquid and bubble-free gas exchange. During the gas replenishment process, the circulating gas can be heated by the electric heater 22, and combined with the continuous drive of the electric fan 23, the gas and temperature inside the chamber can be mixed evenly, ensuring uniform temperature and gas concentration throughout the culture chamber 21. In addition, the gas temperature can be monitored in real time by the temperature sensor 24.

[0040] The above embodiments are only used to illustrate the present utility model and are not intended to limit the technical solutions described in the present utility model. Although the present utility model has been described in detail with reference to the above embodiments, the present utility model is not limited to the specific embodiments described above. Therefore, any modifications or equivalent substitutions to the present utility model, as well as all technical solutions and improvements that do not depart from the spirit and scope of practicality, are covered within the scope of the claims of the present utility model.

Claims

1. A gas concentration control device, comprising a circuit control board (1) and a base plate (16), characterized in that, The circuit control board (1) is connected to a battery (2), an air pump (3), and a circulation pump (11). The output end of the air pump (3) is connected to a first solenoid valve (4) via a silicone tube. The output end of the first solenoid valve (4) is connected to a first three-way pipe (5) via a PU tube. The first three-way pipe (5) is connected to a second three-way pipe (6) via a PU tube. The second three-way pipe (6) is connected to a third three-way pipe (7) via a silicone tube. The second three-way pipe (6) is connected to a proportional valve (8) via a PU tube. The input end of the proportional valve (8) is connected to a second... The input end of the second solenoid valve (9) is connected to an external pipe (10) through a PU tube. A sealing cover (17) is installed on the base plate (16). A connecting plate (18) is installed on the top of the sealing cover (17). A support base (19) is installed and fixed on the base plate (16). An installation base (20) is fixedly connected to the top of the support base (19). A culture chamber (21) is installed and fixed on the top of the installation base (20). An electric heater (22), an electric fan (23), and a temperature sensor (24) are installed and fixed inside the installation base (20).

2. The gas concentration control device according to claim 1, characterized in that, The input end of the circulating pump (11) is connected to a third solenoid valve (12) via a silicone tube. The input end of the third solenoid valve (12) is connected to a third three-way pipe (7) via a silicone tube. The output end of the circulating pump (11) is connected to a carbon dioxide sensor (13) via a silicone tube.

3. The gas concentration control device according to claim 2, characterized in that, The output end of the carbon dioxide sensor (13) is connected to the oxygen sensor (14) through a PU tube. The output end of the oxygen sensor (14) is connected to the fourth solenoid valve (15) through a PU tube. The output end of the fourth solenoid valve (15) is connected to the first three-way pipe (5) through a PU tube.

4. The gas concentration control device according to claim 1, characterized in that, The third tee pipe (7) is connected to the connecting plate (18) via a silicone tube.

5. A gas concentration control device according to claim 1, characterized in that, The inner diameter of the sealing cover (17) is larger than the diameter of the culture chamber (21), and the mounting base (20) is fixed at the bottom center of the culture chamber (21).

6. A gas concentration control device according to claim 1, characterized in that, The electric fan (23) is located in the center of the mounting base (20), and the support base (19) is symmetrically distributed on both sides of the bottom of the mounting base (20).

Images