Method and system for killing bedbugs

Abstract

To provide a bedbug extermination method capable of killing not only larvae and adult bedbugs but also their eggs.SOLUTION: An object to be treated is housed in an airtight treatment chamber formed inside a treatment container. Carbon dioxide gas is supplied into the treatment chamber to replace the air in the treatment chamber with carbon dioxide gas. Over a predetermined treatment period of 7 to 20 days, the carbon dioxide gas concentration in the treatment chamber is maintained at a given preset concentration from 60 vol.% to 80 vol.% inclusive, and the temperature inside the treatment chamber is maintained at a given preset temperature from 15°C to 35°C inclusive.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present disclosure relates to methods for insecticidal control of bed bugs. [Background technology]

[0002] Bedbugs (Cimex lectularius Linnaeus), also known as bedbugs, are blood-sucking insects. After being distributed nationwide, bedbugs were rarely seen for a time, but recently, damage has been increasing again across the country. Bedbugs hide in gaps in walls, cracks in pillars, and gaps and corners of wooden furniture, wandering around at night to suck blood.

[0003] Known methods for exterminating bed bugs include spraying, fumigation, and smoking with insecticides such as pyrethroid compounds, organophosphorus compounds, and carbamate compounds. However, the use of such chemical insecticides is likely to affect organisms other than bed bugs, and is less effective against individuals that are highly resistant to insecticides. Therefore, Patent Document 1 proposes a method for exterminating bed bugs that does not use chemical insecticides.

[0004] The bed bug extermination method described in Patent Document 1 involves placing the item from which bed bugs are to be exterminated inside a container, filling the container with carbon dioxide gas (carbon dioxide gas), sealing the container, and burying the container in an atmosphere of carbon dioxide gas for 10 hours or more, thereby exterminating the bed bugs living inside the item. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Japanese Patent Application Laid-Open No. 2015-167486 Summary of the Invention [Problem to be solved by the invention]

[0006] Female bed bugs lay 2-5 eggs every day, totaling approximately 200 eggs in their lifetime. Bed bug larvae molt five times and emerge as adults within 1-3 months. Adult bed bugs have a lifespan of 10-12 months, and can reproduce at least three times a year.

[0007] Conventional methods of killing bed bugs by fumigating with carbon dioxide gas target larvae and adults, but do not take into consideration the killing of eggs (i.e., ovicidal). If eggs are not eradicated, eggs remaining on furniture or in rooms after treatment may hatch, leading to repeated bed bug infestations.

[0008] The present disclosure has been made in view of the above circumstances, and its object is to provide a method for killing bed bugs that can kill not only larvae and adults of bed bugs but also eggs. [Means for solving the problem]

[0009] In order to solve the above problems, a method for killing bed bugs according to one embodiment of the present disclosure includes: The object to be processed is placed in an airtight processing chamber formed in the processing container; supplying carbon dioxide gas into the processing chamber to replace the air in the processing chamber with carbon dioxide gas; The carbon dioxide gas concentration in the processing chamber is maintained at a predetermined set concentration of 60 vol% or more and 80 vol% or less over a predetermined processing time of 7 days or more and 20 days or less, and the temperature in the processing chamber is maintained at a predetermined set temperature of 15°C or more and 35°C or less.

[0010] In addition, a bed bug extermination system according to one aspect of the present disclosure includes: a processing container having an airtight processing chamber therein for accommodating an object to be processed; a carbon dioxide gas cylinder for supplying carbon dioxide gas to the processing chamber; a supply valve that adjusts the amount of carbon dioxide supplied from the carbon dioxide gas cylinder to the processing chamber; a carbon dioxide concentration meter for detecting a carbon dioxide gas concentration in the processing chamber; a thermometer for detecting the temperature inside the processing chamber; The apparatus is provided with a monitor device that monitors the carbon dioxide gas concentration and temperature in the processing chamber based on the detected values ​​of the carbon dioxide concentration meter and the thermometer so that the carbon dioxide gas concentration in the processing chamber is maintained at a predetermined concentration of 60 vol% or more and 80 vol% or less, and the temperature of the processing chamber is maintained at a predetermined set temperature of 15°C or more and 35°C or less, over a predetermined processing time of 7 days or more and 20 days or less. [Effects of the Invention]

[0011] According to the present disclosure, it is possible to provide a method and system for killing bed bugs that can kill not only larvae and adult bed bugs but also eggs. [Brief explanation of the drawings]

[0012] [Figure 1] FIG. 1 is a diagram showing the overall configuration of a bed bug extermination system for carrying out a bed bug extermination method according to an embodiment of the present disclosure. DETAILED DESCRIPTION OF THE INVENTION

[0013] Next, an embodiment of the present disclosure will be described with reference to the drawings. Fig. 1 is a diagram showing a schematic configuration of a bed bug extermination system 1 for carrying out a bed bug extermination method according to one embodiment of the present disclosure. The bed bug extermination system 1 shown in Fig. 1 includes a treatment container 2, a carbon dioxide gas cylinder 32 that is a carbon dioxide gas source, and a monitor device 4 that monitors the environment inside the treatment container 2. In other words, the bed bug extermination system 1 is a system for performing carbon dioxide gas fumigation treatment of a treatment target W inside the treatment container 2.

[0014] The processing vessel 2 has a processing chamber 20 therein. The processing vessel 2 according to this embodiment is made up of a frame 21 and a gas barrier bag 22 that encases the frame 21. The shape of the frame 21 is not particularly limited, as long as it can form the processing chamber 20 that can accommodate the object to be processed W inside the gas barrier bag 22. The gas barrier bag 22 is made by molding a gas barrier sheet into a bag shape, and is highly airtight when the object to be processed W is placed inside and closed. The processing vessel 2 is not limited to the aspect of this embodiment, and can be any shape that can form the processing chamber 20 that can accommodate the object to be processed W and is airtight against the outside.

[0015] Carbon dioxide gas cylinder 32 is disposed outside processing vessel 2 and is connected to processing chamber 20 via carbon dioxide gas supply pipe 23. Carbon dioxide gas supply pipe 23 is provided with regulator 33 that adjusts the pressure of carbon dioxide gas supplied from carbon dioxide gas cylinder 32 into processing vessel 2 to a predetermined value, and supply valve 24 that adjusts the amount of carbon dioxide gas supplied to processing chamber 20. Processing chamber 20 may be provided with safety valve 25 that releases gas to the outside when the internal pressure reaches or exceeds a predetermined upper limit.

[0016] The processing vessel 2 is provided with various instruments, such as a pressure gauge 31 that detects the pressure in the processing chamber 20, a thermometer 34 that detects the temperature of the processing chamber 20, a hygrometer 35 that detects the humidity in the processing chamber 20, and a carbon dioxide concentration meter 37 that measures the carbon dioxide gas concentration in the processing chamber 20. The number and arrangement of the various instruments are set appropriately depending on the shape and size of the processing chamber 20. For example, the carbon dioxide concentration meters 37 are installed in three locations of the processing chamber 20: on the floor, ceiling, and midway between the floor and ceiling, and the average value of the measurements of these carbon dioxide concentration meters 37 is used as the carbon dioxide gas concentration in the processing chamber 20.

[0017] Preferably, the bed bug extermination system 1 includes an air conditioner 36 that adjusts the temperature of the treatment chamber 20. If the environment inside and outside the treatment chamber 20 meets the temperature requirements for treatment, the air conditioner 36 may be omitted. The type of air conditioner 36 is not particularly limited; however, a preferred type is one that indirectly adjusts the temperature inside the treatment chamber 20 from outside the treatment container 2, such as by adjusting the temperature of the environment in which the treatment container 2 is placed (e.g., the room 10 in which the treatment container 2 is installed). However, the device that adjusts the temperature inside the treatment container 2 may be located inside the treatment container 2. Furthermore, if the object to be treated W (e.g., furniture, artwork, etc.) is sensitive to humidity changes, the bed bug extermination system 1 preferably includes a humidistat 38 that adjusts the humidity inside the treatment chamber 20. In this case, the humidistat 38 may autonomously adjust the humidity inside the treatment chamber 20 using a humidity control agent.

[0018] The monitoring device 4 acquires detection values ​​from various instruments, including a pressure gauge 31, a thermometer 34, a hygrometer 35, and a carbon dioxide concentration meter 37, and monitors process values ​​that indicate the state of the treatment process. The process values ​​include the pressure, temperature, humidity, and carbon dioxide gas concentration of the treatment chamber 20. For example, the monitoring device 4 has a calculator and a display as an output device, and displays and outputs the process values ​​on the display based on the detection values ​​acquired from the various instruments. Also, for example, the monitoring device 4 has a calculator and a display and a speaker as output devices, and outputs an alarm from the display, speaker, etc. if the process value, based on the detection values ​​acquired from the various instruments, falls outside a predetermined set value range.

[0019] The bed bug extermination system 1 can be operated manually, but may also be configured to be capable of automatic operation. When the bed bug extermination system 1 is capable of automatic operation, the monitor device 4 also functions as a process control device that controls the process value of the treatment chamber 20 to a predetermined set value.

[0020] The monitoring device 4, which functions as a process control device, is electrically connected to a pressure gauge 31, a thermometer 34, a hygrometer 35, and a carbon dioxide concentration meter 37, and acquires detected values ​​from these instruments. The monitoring device 4 is also connected to the supply valve 24 and an air conditioning device 36, and can control the operation of these devices.

[0021] The monitor device 4 is configured using a computer equipped with a calculator, a memory, an input device, and an output device. The calculator executes a program stored in the memory, allowing the monitor device 4 to function as a process control device. For example, the monitor device 4 acquires the carbon dioxide gas concentration in the treatment chamber 20 from the carbon dioxide concentration meter 37, and based on the acquired detected value of the carbon dioxide gas concentration, changes the opening of the supply valve 24 to adjust the flow rate of carbon dioxide gas supplied from the carbon dioxide gas cylinder 32 to the treatment chamber 20, thereby controlling the carbon dioxide gas concentration in the treatment chamber 20 to a predetermined set concentration. Furthermore, for example, the monitor device 4 acquires the temperature of the treatment chamber 20 from the thermometer 34, and based on the acquired detected value of the temperature, changes the setting of a temperature control device such as the air conditioner 36 to control the temperature of the treatment chamber 20 to a predetermined set temperature. The monitor device 4 may be configured to control at least one of the carbon dioxide gas concentration and temperature in the treatment chamber 20 to a set value. By having the monitor device 4 function as a process control device in this way, the bed bug extermination system 1 can be remotely monitored and operated.

[0022] How to kill bed bugs Here, a method for exterminating bed bugs using the bed bug extermination system 1 having the above configuration will be described.

[0023] First, the treatment container 2 is placed in a room 10 equipped with an air conditioning device 36, and the object to be treated W is placed in the treatment container 2 and then the treatment container 2 is sealed. Examples of the object to be treated W include furniture, bedding, home appliances, curtains, etc. that were used in the room 10.

[0024] Next, the supply valve 24 is opened to start supplying carbon dioxide gas from the carbon dioxide gas cylinder 32 to the processing chamber 20, thereby replacing a portion of the air in the processing chamber 20 with carbon dioxide gas. The supply rate of carbon dioxide gas is relatively high until the carbon dioxide gas concentration in the processing chamber 20 approaches a predetermined set concentration. Once the carbon dioxide gas concentration approaches the set concentration, the supply rate of carbon dioxide gas is relatively reduced, thereby increasing the carbon dioxide gas concentration in the processing chamber 20 to the set concentration. While carbon dioxide gas is supplied to the processing chamber 20 and air is purged from the processing chamber 20 at the same time, the safety valve 25 or another purge valve may be open. After the carbon dioxide gas concentration in the processing chamber 20 reaches the set concentration, a small amount of carbon dioxide gas may be constantly supplied to the processing chamber 20 to maintain the set concentration, or carbon dioxide gas may be supplied to the processing chamber 20 only when the carbon dioxide gas concentration decreases. While dry carbon dioxide is supplied from the carbon dioxide gas cylinder 32 to the processing chamber 20 in the above example, the processing chamber 20 may be humidified by supplying humidified carbon dioxide gas.

[0025] The set concentration of carbon dioxide gas in the treatment chamber 20 is 60 vol% or more and 80 vol% or less, and more preferably 65 vol% or more and 75 vol% or less. If the carbon dioxide gas concentration in the treatment chamber 20 exceeds 80 vol%, the insecticidal effect may decrease. Also, if the carbon dioxide gas concentration in the treatment chamber 20 is less than 60 vol%, the insecticidal effect may be insufficient. For example, carbon dioxide gas is supplied with the set carbon dioxide gas concentration in the treatment chamber 20 set to 75 vol%, and the carbon dioxide gas concentration is controlled so that it does not fall below 65 vol%, taking into account factors such as leakage and adsorption of carbon dioxide gas.

[0026] The set temperature of the treatment chamber 20 is 15°C or higher, and more preferably 20°C or higher. If the temperature of the treatment chamber 20 is below 15°C, the insecticidal effect will be significantly reduced. There is no particular upper limit to the temperature of the treatment chamber 20, but an excessively high temperature may cause deterioration of the object to be treated W, and from an economical point of view, it is appropriate to set the upper limit at around 35°C. If the temperature of the treatment chamber 20 falls outside the set temperature range, including the set value and the upper and lower allowable ranges, the air conditioning unit 36 ​​is operated to bring the temperature of the treatment chamber 20 within the set temperature range.

[0027] After the carbon dioxide gas concentration in the treatment chamber 20 reaches the set concentration, the process waits for a predetermined treatment time to elapse while monitoring the temperature, humidity, pressure, and carbon dioxide gas concentration in the treatment chamber 20. In other words, the treatment target W is fumigated with carbon dioxide gas for the predetermined treatment time.

[0028] The treatment time is 7 days (168 hours) or more, and more preferably 10 days (240 hours) or more. If the treatment time is less than 7 days, it is possible to kill adult bed bugs and larvae, but there is a risk that the killing of eggs will be insufficient. There is no particular upper limit to the treatment time, but from an economical point of view, a short treatment time is preferable, and a treatment time of 14 days (336 hours) is sufficient, with an upper limit of 20 days (480 hours) being appropriate.

[0029] The monitor device 4 acquires the carbon dioxide gas concentration in the processing chamber 20 detected by the carbon dioxide concentration meter 37 and the temperature of the processing chamber 20 detected by the thermometer 34 over the processing time, and monitors the carbon dioxide gas concentration and temperature of the processing chamber 20. If the detected carbon dioxide gas concentration in the processing chamber 20 is outside a predetermined set concentration (preferably, a set concentration range) of 60 vol% or more and 80 vol% or less, the monitor device 4 notifies the user of an abnormality via a display or speaker. Furthermore, if the detected temperature of the processing chamber 20 is outside a predetermined set temperature (preferably, a set temperature range) of 15°C or more and 35°C or less, the monitor device 4 notifies the user of an abnormality via a display or speaker. Furthermore, the monitor device 4 is equipped with a timer, and when a predetermined processing time of 7 days or more and 20 days or less has elapsed, the monitor device 4 notifies the user of the end of the processing time via a display or speaker.

[0030] After the treatment time has elapsed, the treatment container 2 is opened, and bedbugs and their eggs are removed from the treatment chamber 20 and from the object to be treated W removed from the treatment chamber 20. A suction vacuum cleaner or the like can be used for the removal. Through the above steps, adult bedbugs, larvae, and eggs can be killed in the object to be treated W.

[0031] [Example 1] A test was conducted to evaluate the effectiveness of the above-described method for killing bed bugs.

[0032] -Test method- (1) A frame 21 measuring 3.0 m in length, 3.0 m in width, and 2.0 m in height was constructed using steel pipes in a room maintained at 25°C by an air conditioning unit 36, and items to be treated W, such as furniture, home appliances, curtains, and paintings, were placed inside the frame 21. (2) Test insects and filter paper were placed in plastic cups with an opening of 80 mm and a height of 60 mm, and the opening was covered with nylon gauze. Two cups containing test insect a and test insect b were prepared. Test insect a consisted of 10 adult bed bugs (5 males and 5 females). Test insect b consisted of 5 bed bug eggs laid on filter paper. Cups containing test insect a and test insect b were placed on both the floor and at a height of 2.0 m within frame 21. As controls, cups containing test insect a and test insect b were placed within room 10 but outside treatment room 20. The adult bed bugs used as test insects were collected at a different site one week prior to the test. The bed bug eggs used as test insects were laid in an environment of 25°C within two days prior to the test, kept in an incubator set at 25°C, and removed from the incubator within 24 hours prior to the test. (3) The object to be treated W was placed in a gas barrier bag 22 together with the frame 21, and the gas barrier bag 22 was sealed to form a treatment chamber 20. Carbon dioxide gas was supplied from a carbon dioxide gas cylinder 32 to the treatment chamber 20 without heating or humidifying. The carbon dioxide gas concentration in the treatment chamber 20 was measured using a carbon dioxide concentration meter 37 immediately after the start of carbon dioxide gas replacement and after 1.0, 2.0, 3.0, 3.5, 24, 168, and 336 hours. A high-concentration flammable gas detector (PX-3140, manufactured by New Cosmos Electric Co., Ltd.) was used as the carbon dioxide concentration meter 37. The carbon dioxide gas concentration was measured near the ceiling, near the floor, and midway between the ceiling and floor of the treatment chamber 20, and the average of the detected values ​​at the three locations was taken as the carbon dioxide gas concentration in the treatment chamber 20. The carbon dioxide gas concentration in the treatment chamber 20 before the carbon dioxide gas supply was 0.0 vol%, and the carbon dioxide gas concentration rose rapidly after the start of the supply. The carbon dioxide gas concentration in the processing chamber 20 reached 70 vol% or more 3.5 hours after the start of supply. Subsequent measurements of the carbon dioxide gas concentration also maintained the carbon dioxide gas concentration at 70 vol% or more, reaching 69.0 vol% after 336 hours had passed (i.e., immediately before opening). (4) The treatment time was 14 days, and the treatment object W in the treatment chamber 20 was exposed to carbon dioxide gas for 14 days. A temperature and humidity data logger (Ondotori TR-72wb, manufactured by T&D Corporation) was installed in the treatment chamber 20 during the fumigation treatment, and the temperature and humidity were measured and recorded every hour. The average temperature in the treatment chamber 20 during the fumigation treatment was 25.3±1.1°C, and the average humidity was 60.6±3.3%. In addition, a similar temperature and humidity data logger was installed in the room 10 (outside the treatment chamber 20) during the fumigation treatment, and the temperature and humidity were measured and recorded every hour. The average temperature in the room 10 during the fumigation treatment was 25.5±1.0°C, and the average humidity was 47.4±5.7%. Both the temperature and humidity inside the treatment chamber 20 were stable throughout the treatment period. (5) After the treatment time had elapsed, the gas barrier bag 22 was opened and the cup containing the test insects was removed. Test insect a was observed under a stereomicroscope to confirm whether it was alive or dead. Test insect b was left standing for another week in an incubator set at 25°C, after which it was confirmed whether it had hatched.

[0033] -Test Results- All test insects a (adult bed bugs) in the cups placed in treatment chamber 20 were dead, regardless of their location. Of the cups containing test insect a, one placed near the ceiling had one bloody feces particle attached to the filter paper. None of test insects b (bed bug eggs) placed in treatment chamber 20 hatched, regardless of their location. On the other hand, all test insects a (adult bed bugs) in the cups placed in the control area (i.e., in room 10) survived, and a large number (15 particles) of bloody feces particle attached to the filter paper. In addition, all test insects b (bed bug eggs) placed in the control area were confirmed to have hatched. After the treatment time had elapsed, two dead bed bugs were found on the floor of treatment chamber 20.

[0034] This test confirmed that the method of exterminating bed bugs using carbon dioxide gas fumigation is effective in killing both adult and egg bed bugs. It is said that even the same insect has different resistance to carbon dioxide depending on its developmental stage. Although no tests were conducted on bed bug larvae, it can be assumed that the same effect as on adult and egg bed bugs can be applied. Furthermore, based on the number of bloody feces found on the filter paper, it is highly likely that the adult bed bugs turned over or died relatively quickly, specifically within a few hours.

[0035] [Example 2] In the above-mentioned method for killing bed bugs, a test was conducted to verify the treatment time of carbon dioxide gas fumigation treatment that can kill bed bug eggs.

[0036] -Test method- (1) Five bed bug eggs laid on filter paper were prepared as test insects, and the test insects were placed together with the filter paper in a plastic cup with a diameter of 80 mm and a height of 60 mm. The test bed bug eggs were laid in an environment of 25°C within two days prior to the test, kept in an incubator set at 25°C, and removed from the incubator within 24 hours prior to the test. (2) The cup containing the test insect was placed in a treatment chamber 20 formed in a gas barrier bag 22. Carbon dioxide gas was supplied from a carbon dioxide gas cylinder 32 to the treatment chamber 20 without heating or humidifying. The temperature of the treatment chamber 20 was maintained at 25°C, and the carbon dioxide gas concentration was maintained at 70 vol% or more and 75 vol% or less for 7 days. (3) The gas barrier bag 22 was opened and the cup containing the test insects was taken out. The test insects were left to stand for another week in an incubator set at 25°C, and then it was confirmed whether or not they had hatched.

[0037] -Test Results- None of the test insects (i.e., bed bug eggs) hatched. This test confirmed that bed bug eggs can be killed by a 7-day carbon dioxide gas fumigation treatment.

[0038] [Summary] The method for killing bed bugs according to the first aspect of the present disclosure comprises: The processing object W is accommodated in an airtight processing chamber 20 formed in the processing container 2, Carbon dioxide gas is supplied to the processing chamber 20 to replace the air in the processing chamber 20 with carbon dioxide gas; For a predetermined processing time of 7 days or more and 20 days or less, the carbon dioxide gas concentration in the processing chamber 20 is maintained at a predetermined set concentration of 60 vol% or more and 80 vol% or less, and the temperature inside the processing chamber 20 is maintained at a predetermined set temperature of 15°C or more and 35°C or less.

[0039] According to the above method, bed bugs can be eradicated by killing not only adult and larval bed bugs but also bed bug eggs.

[0040] The bed bug extermination method according to the second item of the present disclosure is the bed bug extermination method according to the first item, wherein the treatment time is 7 days or more and 14 days or less, the set temperature is 25°C or more and 35°C or less, and the set concentration is 65 vol% or more and 75 vol% or less.

[0041] According to the above method, adult bedbugs, larvae, and eggs can be effectively killed.

[0042] The bed bug extermination system 1 according to the third aspect of the present disclosure includes: a processing vessel 2 having an airtight processing chamber 20 therein for accommodating an object to be processed W; a carbon dioxide gas cylinder 32 for supplying carbon dioxide gas to the processing chamber 20; a supply valve 24 for adjusting the amount of carbon dioxide supplied from the carbon dioxide gas cylinder 32 to the processing chamber 20; a carbon dioxide concentration meter 37 for detecting the carbon dioxide gas concentration in the processing chamber 20; a thermometer 34 for detecting the temperature inside the processing chamber 20; The apparatus is provided with a monitor device 4 that controls at least one of the carbon dioxide gas concentration and temperature in the processing chamber 20 based on the detection values ​​of the carbon dioxide concentration meter 37 and the thermometer 34 so that the carbon dioxide gas concentration in the processing chamber 20 is maintained at a predetermined set concentration of 60 vol% or more and 80 vol% or less, and the temperature of the processing chamber 20 is maintained at a predetermined set temperature of 5°C or more and 35°C or less, over a predetermined processing time of 7 days or more and 20 days or less.

[0043] The bed bug extermination system 1 provides a method for effectively exterminating bed bugs by killing not only adult and larval bed bugs but also bed bug eggs.

[0044] The above describes preferred embodiments of the present disclosure, but the present disclosure may also include modifications to the specific structure and / or function details of the above embodiments, provided that the modifications do not deviate from the spirit of the present disclosure. [Explanation of symbols]

[0045] 1: Bed bug extermination system 2: Processing container 20: Processing room W: Processing object

Claims

1. The object to be processed is placed in an airtight processing chamber formed in the processing container; supplying carbon dioxide gas into the processing chamber to replace the air in the processing chamber with carbon dioxide gas; maintaining the carbon dioxide gas concentration in the treatment chamber at a predetermined set concentration of 60 vol% or more and 80 vol% or less, and maintaining the temperature in the treatment chamber at a predetermined set temperature of 15°C or more and 35°C or less, for a predetermined treatment time of 7 days or more and 20 days or less; How to kill bed bugs.

2. the treatment time is 7 days or more and 14 days or less, the set temperature is 20°C or more and 35°C or less, and the set concentration is 65 vol% or more and 75 vol% or less; The method for killing bedbugs according to claim 1.

3. a processing container having an airtight processing chamber therein for accommodating an object to be processed; a carbon dioxide gas cylinder for supplying carbon dioxide gas to the processing chamber; a supply valve that adjusts the amount of carbon dioxide supplied from the carbon dioxide gas cylinder to the processing chamber; a carbon dioxide concentration meter for detecting a carbon dioxide gas concentration in the processing chamber; a thermometer for detecting the temperature inside the processing chamber; a monitor device that controls at least one of the carbon dioxide gas concentration and the temperature in the processing chamber based on the detected values ​​of the carbon dioxide concentration meter and the thermometer so that the carbon dioxide gas concentration in the processing chamber is maintained at a predetermined set concentration of 60 vol% or more and 80 vol% or less, and the temperature of the processing chamber is maintained at a predetermined set temperature of 15°C or more and 35°C or less, over a predetermined processing time of 7 days or more and 20 days or less, Bed bug killing system.

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