GERMICIDAL COMPOSITIONS FOR IMPROVED BIOCIDE RECOVERY AND EFFICACY

Abstract

A germicidal composition includes a biocide selected from a benzyl-substituted quaternary ammonium compound (QAC) and a release agent selected from a non-benzyl-substituted quaternary ammonium cationic surfactant. The release agent may optionally also include an amphoteric amine surfactant. A sterilization product may include a substrate and at least one biocide of a germicidal composition impregnating the substrate, although one or more other components of the germicidal composition may be bound to or otherwise impregnating the substrate. A method may include contacting a substrate with a germicidal composition and / or modulating one or more biocides and release agents to prepare a germicidal composition, a sterilization product, or any combination thereof.

Description

GERMICIDAL COMPOSITIONS FOR IMPROVED BIOCIDE RECOVERY AND EFFICACY CROSS REFERENCE TO RELATED APPLICATIONS This application claims priority to United States Provisional Patent Application No. 62 / 906,900, filed on September 27, 2019, the contents of which are incorporated herein by reference in their entirety. TECHNICAL FIELD The modalities relate to germicidal compositions that include a biocide and a release agent for improved biocide recovery in the expressed fluid from a substrate and consequently improved biocide efficacy for sterilization. BACKGROUND Biocides, such as cationic antimicrobial biocides, have been incorporated into compositions for sterilizing a variety of surfaces. For example, a quaternary ammonium compound and a biguanide or bisbiguanide can be used to sterilize surfaces. Meanwhile, a quaternary ammonium compound and a polyvinyl alcohol or a poloxamer can be used with wipes (e.g., cellulose-based fibrous cloths) to sterilize surfaces. Such compositions, however, may be ineffective at inhibiting the absorption of specific biocides into the wipes or may increase the cost and complexity of the sterilization technology. Wipes can also be coated with a crosslinked release agent that forms a structural network which physically adheres to the wipe, along with a permeator that counteracts a blocking effect on leached crosslinked release agents.While such compositions can inhibit the absorption of certain biocides into wipes, pre-treatment with a crosslinked release agent and a permeabilizer can increase cost and complexity. Therefore, there is considerable room for improvement in providing less expensive or less complex yet effective sterilization technologies. BRIEF DESCRIPTION The modalities relate to germicidal compositions that include a biocide and a release agent. For example, a germicidal composition comprises (i) a biocide selected from a benzyl-substituted quaternary ammonium compound (QAC) and (ii) a release agent selected from a non-benzyl-substituted QAC. The release agent may further include an amphoteric amine surfactant. Notably, the recovery of a biocide from the expressed fluid of a fibrous substrate can be modulated by changing the concentration of the release agent in the germicidal composition. In this way, a skilled operator could finely adjust the amount of biocide delivered using a release agent to maximize the biocide's efficacy while minimizing the cost and / or complexity of the sterilization technology. The ratio of a release agent to a biocide can be approximately 0.5:1 to approximately 3:1. Additionally or alternatively, a biocide can be present in an amount of approximately 0.01% to approximately 0.5% by weight, based on the total weight of a germicidal composition. Additionally or alternatively, a release agent can be present in an amount of approximately 0.1% to approximately 1.0% by weight, based on the total weight of a germicidal composition. For example, a release agent can be present in an amount of approximately 0.2% to approximately 0.6% by weight, based on the total weight of a germicidal composition, and a biocide can be present in an amount of approximately 0.1% to approximately 0.4% by weight, based on the total weight of the germicidal composition. In any of the preceding embodiments, a benzyl-substituted QAC can be selected from alkyl dimethyl benzyl ammonium chloride (ADBAC), diisobutylphenoxythioxyethyl dimethyl benzyl ammonium chloride, alone or in any combination thereof. For example, a germicidal composition may include Ce-Cis ADBAC alone, diisobutylphenoxythioxyethyl dimethyl benzyl ammonium chloride alone, or in any combination thereof. In any of the preceding embodiments, a non-benzyl-substituted QAC may be selected from a non-benzyl-substituted quaternary ammonium cationic surfactant, a non-benzyl-substituted quaternary ammonium cationic silicone, a non-benzyl-substituted quaternary ammonium cationic phospholipid, alone or in any combination thereof. For example, a germicidal composition may include a non-benzyl-substituted quaternary ammonium halide cationic surfactant, such as, for instance, Dihydroxypropyl Polyethylene Glycol (PEG)-5 Linoleammonium Chloride, alone or in combination with one or more other non-benzyl-substituted QACs. The release agent may also include an amphoteric amine surfactant. For example, disodium cocoamphodiacetate may be present in a mixture with a non-benzyl-substituted quaternary ammonium cationic phospholipid. In any of the preceding forms, a germicidal composition may include other ingredients such as a solvent, preservative, preservative booster, emollient, pH adjuster, fragrance, chelating agent, foaming agent, extract, alone or in any combination thereof. The other ingredients may be present in an amount of approximately 98.5% to approximately 99.9% by weight, based on the total weight of a germicidal composition. The embodiments further relate to a wipe comprising (i) a fibrous substrate and (ii) a germicidal composition impregnating the fibrous substrate. For example, a wipe includes a germicidal composition comprising (i) a biocide selected from a benzyl-substituted QAC and (ii) a release agent selected from a non-benzyl-substituted QAC. Additionally or alternatively, at least an initial ratio of a release agent to a biocide may be approximately 0.5:1 to approximately 3:1. For example, a ratio of a release agent to a biocide when a germicidal composition (e.g., a bulk solution) first makes contact with a fibrous substrate (e.g., a wipe) may be approximately 0.5:1 to approximately 3:1. The modalities also relate to methods for preparing germicidal compositions, sterilization products, or any combination thereof. For example, a method for preparing a germicidal composition may include mixing (i) a biocide and (ii) a release agent. Additionally or alternatively, a method for preparing a sterilization product (for example, a wipe) may include combining (i) a substrate (for example, a fibrous substrate) and (ii) a germicidal composition. For example, a method for preparing a wipe may include applying a germicidal composition, having a release agent-to-biocide ratio of approximately 0.5:1 to approximately 3:1, to a fibrous substrate. The germicidal composition and the fibrous substrate may be packaged in the same container. The methods also relate to modulating the recovery of a biocide in the expressed fluid of a sterilization product by (i) keeping the amount of one biocide or release agent substantially constant and varying the amount of the other biocide or release agent, or (ii) modulating the amount of the biocide and the release agent simultaneously. For example, a method for modulating the recovery of Benzalkonium Chloride in the expressed fluid of wipes might include varying the amount of a release agent in the germicidal composition applied to the wipes at constant levels of Benzalkonium Chloride. The modalities also relate to methods for using germicidal compositions, sterilizing products, alone or in any combination thereof. For example, a surface (e.g., an outer film) can be sterilized by placing the surface in contact with a wipe that has a germicidal composition of any of the preceding modalities. BRIEF DESCRIPTION OF THE DRAWINGS The features described are believed to be novel, and the features of elements of the invention are set forth in particular in the appended claims. The figures are for illustrative purposes only and are not drawn to scale. The description itself, however, as well as the organization and method of operation, may be better understood by reference to the description of the preferred embodiment(s) that follows, taken in conjunction with the accompanying drawings, in which: FIG. 1 is a graph showing the effect of various release agents on the recovery of Benzalkonium Chloride in the expressed fluid of a wipe according to the modalities; and FIGS. 2A-2B are graphs showing the in vitro time extermination evaluations of various germicidal compositions having Benzalkonium Chloride on gram-positive bacteria and gram-negative bacteria, respectively, according to the modalities. DETAILED DESCRIPTION The formulations may comprise, consist of, and essentially consist of the features and / or steps described herein, as well as any additional or optional ingredients, components, steps, or limitations described herein or otherwise appreciated by one skilled in the art. All concentrations described herein are understood to be percent by weight (% wt) based on the total weight of the composition unless otherwise indicated. Where appropriate, the International Nomenclature of Cosmetic Ingredients (INCI) name of ingredients / components is provided. Any range quoted herein is intended to include all sub-ranges encompassed therein, and such ranges are understood to include each and every number and / or fraction between the lower and upper values ​​of the stated range. Amine Compounds Amine compounds are characterized as primary (1°), secondary (2°), tertiary (3°), or quaternary (4°) based on how many substituents (e.g., alkyl substituents) are attached to the nitrogen atom. Additionally, a quaternary ammonium compound (QAC) is characterized by the general formula R₄N⁺ in the cationic form and by the general formula RuN⁺X⁻ in the salt form, where (Bu) refers to the four substituents on the nitrogen atom and (X⁻) refers to the counterion. The substituents can be, for example, substituted or unsubstituted alkyl, aryl, alkylaryl, arylalkyl, cycloalkyl (aromatic or non-aromatic), heterocyclyl, and / or alkenyl groups; and can be straight-chain, branched-chain, interrupted, or uninterrupted. Furthermore, two or more of the R4 substituents can together with the nitrogen atom form a substituted or unsubstituted heterocyclic ring.Disrupting groups can include heteroatoms such as oxygen, nitrogen, sulfur, and phosphorus-containing groups (e.g., phosphinate). The much more common counterion is chloride, although other halides (e.g., fluoride, bromide, iodide, etc.) and non-halide ions (e.g., sulfonate, saccharin, etc.) can be used. Biocide Ideally, the biocide should be a QAC (Quality Action Chemical) with sufficient germicidal potency to eliminate microorganisms such as viruses, bacteria, yeast, and similar organisms. The biocide can be applied to a surface to disinfect or sterilize it. For example, it can be used to disinfect or sterilize an outer film, an implement (e.g., a medical tool), a floor, a wall, a table, an inanimate object (e.g., a medical glove, a house surface, a car surface, a piece of furniture), or any other surface requiring sterilization. Preferably, the biocide is selected from a benzyl-substituted QAC, and even more preferably from a dimethyl benzyl-substituted QAC. In this respect, at least one of the four substituents on the quaternary ammonium cation group is a benzyl group, and preferably at least two of the four substituents on the quaternary ammonium cation group are methyl groups. A dimethyl benzyl-substituted QAC can be characterized by the following structure: where R is any substituted, unsubstituted, straight-chain, branched-chain, interrupted, and / or uninterrupted group. Furthermore, the benzyl group is either substituted or unsubstituted. The dimethyl benzyl-substituted QAC may include a mixture of QACs such as, for example, one or more of the following: alkyl dimethyl benzyl chloride (ADBAC or Benzalkonium Chloride) which includes C14 C16 C18 C12 C14 C16 C12 C14 C16 C18 C12 C14 C16 C12 C14 C16 C18 C12 C14 Cs-io C14 C16 C12 C14 C16 C12 C14 C16 C12 C14 C16 C18 C12 C14 C16 C18 C12 C14 C16 C12 C12 C14 C16 of ADBA that includes C12 C12 alkyl dimethyl ethylbenzyl chloride (ADEBAC) C14 (32%) , C12 (50%) C14 (30%) Ci (17%) Cis (3%) ; n> ω N C N N CC «L U alkyl dimethyl 1-naphthylmethyl ammonium chloride including C12 (98%) C14 (2%); n-alkyl dimethyl dimethyl ammonium chloride including C12 (68%) C14 (32%); dodecyl benzyl trimethyl ammonium chloride; diisobutylphenoxyethoxyethyl dimethyl benzyl ammonium chloride (Benzethonium Chloride); methyl dodecyl benzyl trimethyl ammonium chloride (80%) methyl dodecyl xylene bis trimethyl ammonium chloride (20%); and / or diisobutyl cresoxyethoxyethyl dimethyl benzyl ammonium chloride monohydrate. Therefore, the biocide may include an alkyl dimethyl benzyl ammonium halide such as Cg-Cis ADBAC, a diisobutylphenoxythioxyethyl dimethyl benzyl ammonium halide such as diisobutylphenoxythioxyethyl dimethyl benzyl ammonium chloride, alone or any combination thereof. For example, the germicidal composition may include only one or more Ce-Cis ADBACs, only diisobutylphenoxythioxyethyl dimethyl benzyl ammonium chloride, or a combination of one or more Cs-Cis ADBACs as a first biocide and diisobutylphenoxythioxyethyl dimethyl benzyl ammonium chloride as a second biocide. The biocide may be present in an amount of approximately 0.01% to approximately 0.5% by weight, based on the total weight of the germicidal composition. For example, the biocide may be present in an amount of 0.008%, 0.15%, 0.3%, 0.55%, or 0.6% by weight, including all numbers and / or fractions in between, based on the total weight of the germicidal composition. For example, the germicidal composition may include one or more Cs-Cis ADBACs present in an amount of approximately 0.01% by weight to approximately 0.5% by weight, diisobutylphenoxythioxyethyl dimethyl benzyl ammonium chloride present in an amount of approximately 0.01% by weight to approximately 0.5% by weight, or a combination of one or more Cb-Cis ADBACs as a first biocide and diisobutylphenoxythioxyethyl dimethyl benzyl ammonium chloride as a second biocide present in a total amount of approximately 0.01% by weight to approximately 0.5% by weight, based on the total weight of the germicidal composition. Liberation Agent Unexpectedly, the recovery of a biocide in the expressed fluid of a fibrous substrate can be modulated as the concentration of the release agent in a germicidal composition changes. In fact, the observed recovery of a biocide as a function of increasing the release agent is contrary to any conventional expectation given the changes with relative concentrations. Unrelated to any theory, a release agent can be selected which, in effective quantities, reduces the biocide's binding to an anionic fibrous substrate by competing for electrostatic binding sites, while synergistically allowing the biocide to impregnate more of the fibrous substrate and / or occupy less of the substrate's surface. The release agent can also be non-crosslinked and / or present in a homogeneous mixture with the biocide.Furthermore, the biocide can be selected to have a higher germicidal potency than the release agent (if any). The biocide can be selected to provide, in effective quantities, a greater than 2-log kill rate (a 99% reduction from 1,000,000 colony-forming units [CEUs] to 10,000 CEUs), such as a 3-log kill rate (a 99.9% reduction from 1,000,000 CEUs to 1,000 CEUs), a 6-log kill rate (e.g., a 99.9999% reduction from 1,000,000 CEUs to 1 CEUs), and so on. In this way, the effectiveness of the biocide can be maximized while minimizing the cost and / or complexity of the sterilization technology. The release agent may be selected from a non-benzyl-substituted quaternary ammonium cation (QAC). In this respect, none of the R4 substituents of the quaternary ammonium cation are benzyl groups (i.e., no C6H5CH2 group is directly covalently bonded to the nitrogen atom), but it may otherwise include any substituted, unsubstituted, straight-chain, branched-chain, interrupted, and / or uninterrupted group, including, for example, an R4 substituent having a benzyl group spaced apart from the nitrogen atom. The non-benzyl-substituted QAC may be selected from a non-benzyl-substituted quaternary ammonium cationic surfactant, a non-benzyl-substituted quaternary ammonium cationic silicone, a non-benzyl-substituted quaternary ammonium cationic phospholipid, alone or in any combination thereof. Preferably, the non-benzyl-substituted quaternary ammonium cationic surfactant, in its salt form, is selected from PEG-5 Dihydroxypropyl Linoleammonium Chloride, Behentrimonium Chloride, Centrimonium Chloride, Dicetyldimonium Chloride, Bishydroxyethyl Dihydroxypropyl Stearammonium Chloride, alone or in any combination thereof. Preferably, the non-benzyl-substituted quaternary ammonium cationic silicone is selected from a quaternary siloxane, a quaternium silicone, alone or in any combination thereof. The quaternary siloxane may be selected from, for example, a quaternary alkyl siloxane such as Quaternary Polydimethylsiloxane (Silquat® Di-10, Siltech Corporation). Quaternium silicone can be selected from, for example, Quaternium-3 Silicone, Quaternium-8 Silicone, Quaternium-16 Silicone, Quaternium-17 Silicone, Quaternium-20 Silicone, Quaternium-22 Silicone, alone or any combination thereof.For example, quaternium silicone can be Quaternium Silicone-20 and Propylene Glycol (SilPlex® J2-S-PG, Siltech Corporation). Preferably, the non-benzyl-substituted quaternary ammonium cationic phospholipid, in its salt form, is selected from Cocamidopropyl PG-Dimonium Chloride Phosphate, Linoleamidopropyl PG-Dimonium Chloride Phosphate, Sunflower Seed Chloride Phosphate, Olivamidopropyl PG-Dimonium Chloride Phosphate, Stearamidopropyl PG-Dimonium Chloride Phosphate, Ricinolamidopropyl PG-Dimonium Chloride Phosphate, Dilinolamidopropyl PG-Dimonium Chloride Phosphate, Borageamidopropyl PG-Dimonium Chloride Phosphate, Gravaamidopropyl PG-Dimonium Chloride Phosphate, and Myristamidopropyl PG-Dimonium, alone or any combination thereof.In addition to any of the above non-benzyl-substituted QACs or derivatives thereof, complexes of the above types of non-benzyl-substituted QACs are contemplated, such as PEG-8 Dimethicone Linoeamidopropyl PG-Dimodium (e.g., a phospholipid and a silicone). The above examples of non-benzyl-substituted QACs are commercially available, such as those sold under the trade name Cola® (Colonial Chemical). It should also be understood that other non-benzyl-substituted QACs, including those with counterions other than chloride, can be used as the release agent. The release agent may also include an amphoteric amine surfactant, which has a nitrogen atom and simultaneously possesses hermaphroditic ions to form either a cation or an anion. An amphoteric amine surfactant may have a weak nitrogen atom, such as secondary and tertiary amine groups that can only acquire a positive charge upon protonation, and / or it may have a weak nitrogen atom, such as a betaine group with a positively charged cationic functional group that does not bear a hydrogen atom and a negatively charged functional group (e.g., a carboxylate group that may not be adjacent to the cationic site).The amphoteric amine surfactant can be selected from fatty aminopropionates, characterized by the general formula R-NH-(CH2)2-COONa, fatty iminodipropionates, characterized by the general formula RN-((CH2)2-COONa)2, alkyl betaines such as alkyl dimethyl betaines characterized by the general formula RN-(CH3)2-CH2-COO, alkyl amidopropyldimethyl betaines, characterized by the general formula R-CO-NH(CH2)3-N-(CH3)2-CH2-COO, amphoteric imidazoline derivatives, alkyl polyamino carboxylates characterized by four or more nitrogen atoms and four or more carboxylic acid groups, alone or in any combination thereof. Preferably, the amphoteric amine surfactant is selected from Disodium Cocoamphodiacetate, Cocamidopropyl Hydroxysultaine, Disodium Lauroamphodiacetate, Lauryl Hydroxysultaine, Sodium Bis-Hydrocytylglycinate Crosspolymer, Sodium CocoGlycosides, Sodium Cocoamphoacetate, Cocoamphopropionate of Sodium, Lauroamphoacetate of Sodium, Sodium Stearoamphoacetate, Cetyl Betaine, Cocamidopropyl Betaine, Cocamidopropyl Betaine, Coco Betaine, Lauramidopropyl Betaine, Lauryl Betaine, Polyethylene Glycol, PEG-8 PG-Cocoglucoside, Dimethicone, alone or in any combination thereof. The above examples of infant formulas are commercially available, such as those sold under the trade name Cola®. The release agent may be present in an amount of approximately 0.1% to approximately 1.5% by weight, based on the total weight of the germicidal composition. For example, the release agent may be present in amounts of 0.08%, 0.5%, 1.0%, 1.2%, or 1.8% by weight, including all numbers and / or fractions in between, based on the total weight of the germicidal composition. Furthermore, the ratio of the release agent to the biocide may be approximately 0.5:1 to approximately 3:1. For example, the ratio may be 0.4, 1.0, 2.0, or 3.6 parts of the release agent, including all numbers and / or fractions in between, to 0.8 or 1.2 parts of the biocide, including all numbers and / or fractions in between. In one example, the germicidal composition may include PEG-5 Linoleammonium Dihydroxypropyl Chloride present in an amount of approximately 0.1% by weight to approximately 1.0% by weight, Behentrimonium Chloride present in an amount of approximately 0.1% by weight to approximately 1.0% by weight, or a combination of. Dihydroxypropyl PEG-5 Linolamonium Chloride and Behentrimonium Chloride present in a total amount of approximately 0.1% to approximately 1.0% by weight, based on the total weight of the germicidal composition. In a further example, the germicidal composition may include PEG-8 Dimethicone Linolamidopropyl PG-Dimodium alone, present in a total amount of approximately 0.1% to approximately 1.0% by weight, or in combination with an amphoteric amine surfactant and / or one or more other non-benzyl-substituted QACs. In a further example, the germicidal composition may include Disodium Cocoamphodiacetate present in an amount of approximately 0.1% to approximately 1.0% by weight, Cocamidopropyl PG-Dimodium Chloride Phosphate present in an amount of approximately 0.1% to approximately 1.0% by weight.0% by weight, or a combination of Disodium Cocoamphodiacetate and Cocamidopropyl PG-Dimonium Chloride Phosphate present in a total amount of approximately 0.1% by weight to approximately 1.0% by weight, based on the total weight of the germicidal composition. Other Ingredients The germicidal composition may include other ingredients such as a solvent, preservative, preservative booster, emollient, pH adjuster, fragrance, chelating agent, extract, cleanser, foaming agent, antioxidant, ultraviolet (UV) absorber, or dye, alone or in any combination thereof. These other ingredients may be present in an amount of approximately 98.5% to approximately 99.9% by weight, based on the total weight of the germicidal composition. For example, water (e.g., deionized, potable, etc.) may be present in an amount of at least 85% by weight, and alcohol may be present in an amount up to 14.9% by weight, based on the total weight of the germicidal composition. Alcohol may also be present in an amount greater than 15.0% by weight, such as 15.1% by weight, 25% by weight, 50% by weight, 75% by weight, or 99% by weight.9% by weight, and each number and / or fraction thereof, based on the total weight of the germicidal composition. Solvents are substances that dissolve a solute (a chemically distinct liquid, solid, or gas), resulting in a solution. Preferably, the solvent is selected from inorganic solvents such as water and oxygenated solvents such as alcohol (e.g., ethanol, propanol, etc.), although any volatile or non-volatile solvent may be present. A preservative system may include a preservative, which is a substance used to prevent the growth of harmful microorganisms, alone or in conjunction with a preservative booster, which is a substance used to enhance the effectiveness of the preservative and / or reduce the amount needed to preserve a formulation. Preferably, preservative systems include phenoxyethanol, potassium sorbate, caprylyl glycol, chlorphenesin, and / or parabens (e.g., methylparaben, ethylparaben, propylparaben, etc.), although any preservative and / or booster may be present. Emollients are substances used to soften or smooth the outer film. Preferably, the emollient is selected from a silicone polymer such as PEG-8 dimethicone, although any silicone polymer emollient and / or other emollient may be present, such as, for example, plant oils, mineral oil, shea butter, cocoa butter, petrolatum, fatty acids (animal oils, including emu, mink, lanolin, etc.), triglycerides, benzoates, myristates, palmitates, stearates, etc. pH adjusters are substances used to adjust the pH of a finished product, such as a germicidal composition.Preferably, the pH adjuster is selected from an organic acid such as citric acid, although any other organic acid and / or other pH adjuster may be present, such as, for example, a hydroxide (e.g., sodium, calcium, magnesium, potassium, etc.). Chelating agents are substances used to bind metal ions or metal compounds, preventing them from adhering to a surface such as an outer film. Preferably, the chelating agent is selected from an aminopolycarboxylic acid such as ethylenediaminetetraacetate. Disodium EDTA, although any chelating agent may be present, such as tetrahydroxypropyl ethylenediamine. Cleaners are substances used to remove undesirable residues (e.g., dirt, dust, mess, etc.), odors, and oils / greases (degreasers) from surfaces. Preferably, the cleaner is selected from PEG derivatives of lanolin such as PEG-60 Lanolin, an amphoteric amine surfactant such as Sodium Caprylamphopropionate, alone or in any combination thereof, although any other cleaner may be present. Foaming agents are substances used to facilitate foam formation. Preferably, the foaming agent is a surfactant that includes, but is not limited to, an anionic surfactant selected from Sodium Laureth Sulfate, Sodium Lauryl Sulfate, alone or in any combination thereof, although any other foaming agent may be present.Extracts are substances used for a variety of purposes, such as antioxidant and conditioning. Preferably, the extract is selected from fruit extract, flower extract, or plant extract (e.g., Aloe Barbadensis Leaf Juice), alone or in combination, although other extracts may be present. Fragrances are substances that impart a distinct aroma and can be derived from petroleum or natural raw materials. In addition to fragrance ingredients, > Other ingredients may include UV absorbers, dyes, pigments, and so on, such as metal oxides, chemical sunscreens, physical sunscreens, mineral-based colorants, etc. Sterilization Products Sterilization products (e.g., mops, wipes, pads, etc.) can be made from synthetic, natural, and / or biodegradable fabrics. Traditional fabrics, for example, can be manufactured by weaving together fibers of silk, cotton, polyester, wool, and similar materials to form an interlocking matrix of loops. Nonwoven fabrics, for example, can be manufactured by pressing a single sheet of material from a mass of separate fibers. Fibers such as cotton and rayon can be used in this process, as well as plastic resins such as polyester, polyethylene, and polypropylene. Examples of traditional nonwoven fabrics include meltblown fabrics, spunbond fabrics, carded fabrics, moistened fabrics, and air-laid fabrics, alone or in any combination thereof. Meltblown fabrics can be formed when a molten thermoplastic material is extruded through multiple capillaries as molten fibers in converging high-speed air currents. These air currents attenuate the fibers of the molten thermoplastic material, which are then deposited onto a collection surface to form a fabric of randomly dispersed meltblown fibers. Spun fabrics can be formed by extruding a molten thermoplastic material through multiple single-spindle capillaries, with the diameter of the extruded fibers then rapidly reduced by spunbonding processes.Carded fabrics can be formed when staple fibers are fed through a combing or carding unit, which separates or breaks them into pieces and aligns the staple fibers in the machine direction to form a fibrous, machine-oriented nonwoven fabric that can then be bonded. Wetted fabrics can be formed when fibers are made into liquid suspensions with water and other chemicals, and the resulting paste is pressed into flat sheets by rollers, dried to form rolls of fabric, processed, cut into narrow widths, and punched into individual sheets. Air-laid fabrics can be formed when bundles of fibers are separated, introduced into an air supply, deposited onto a forming surface, and bonded. Meanwhile, the germicidal solution can be prepared in large batch tanks for proportional increase. The tank can be charged with the first ingredient (e.g., water) and optionally heated during preparation to facilitate the mixing of powders to be dissolved or other solids to be melted. Preferably, a relatively cool process can be used, which requires less energy (no heating during batch separation). The other ingredients of the germicidal solution can then be added sequentially and mixed until homogeneous. Notably, germicidal solutions are stable over a wide temperature range (e.g., approximately 4°C to approximately 50°C). In one embodiment, a wipe is formed when a fabric is thrown into the air (e.g., wood pulp) and a carded fabric (e.g., rayon, polyester, etc.) is mixed with it.) are combined to form a fibrous substrate that can be impregnated with the germicidal composition, wherein the wipe is at least partially anionic to provide electrostatic bonding sites. Wipes, for example, can be fed from storage rolls into coating machinery where the germicidal composition is applied by running the wipe through a channel of the germicidal composition, spraying the wipe with the germicidal composition from a series of nozzles, pouring the germicidal composition onto the wipe, etc. For example, a roll of wipes can be placed in a container (e.g., a plastic container, etc.) and the germicidal composition can be injected into the container and onto the roll of wipes. Wipes (e.g., individual towels) can also be placed in a sealed aluminum pouch container by feeding laminated paper sheets. In automated equipment, the wipes are folded into a small pouch, heat-sealed on three sides to form an open wrapper, fed into the pouch, and the germicidal composition is injected into the wipe (e.g., via a conduit through a filler bar). Alternatively, rolls of fabric can be slit lengthwise, saturated with the germicidal composition, cut into pieces of the desired size, and the resulting saturated wipe surrounded by aluminum foil. A hinged cover can then be added to the plastic container and closed, or one or more heat seals can seal the foil to form an airtight pouch (e.g., a final seal for an open wrapper, a three-sided heat seal around the saturated wipe, etc.).The preferred containers are those commonly used for packaging commercially available wipes under the Wet Ones® brand (Edgewell Personal Care Brands, LLC), although any dispenser, such as a reusable wipe dispenser, can be used to package wipes, and any dispenser, such as a bottle, can be used independently to package the germicidal composition. In this way, the sterilization product ingredients can be mixed and / or packaged together, or separately and mixed as needed. Additionally, the amount of germicidal composition deployed in and / or from a sterilization product can be easily determined for a given substrate by modulating one or more of the biocide and / or release agent. This can be done by varying the amount of one biocide or release agent at the same time, or by keeping the amount of one biocide or release agent substantially constant while varying the amount of the other, and then evaluating the expressed fluid from the given substrate to determine the levels of recovered biocide. In this way, a predetermined efficacy can be provided based on a predetermined usage level of the germicidal composition, biocide, and / or release agent. While wipes are described in more detail below, germicidal compositions can be used in any sterilization product, such as mops, pads, sponges, etc. Preferably, anionic substrates are used, which include a surface at least partially anionicly charged (e.g., by means of a coating, an atom or chemical group that provides an anionic charge, etc.). For example, a cellulose sponge functionalized with anionic chemical groups can be mixed with a germicidal composition to provide a cellulose sponge impregnated with the germicidal composition. Additionally, a sterilization product can be a non-foaming product.For example, an anionic substrate (e.g., a non-woven wipe) can be in contact with and / or impregnated with a germicidal solution (e.g., in a container) to provide a non-foaming sterilization product (e.g., a non-foaming wet wipe). In this way, some sterilization product formulations may not negatively affect certain ingredients (e.g., surfactants, etc.) and / or may not require features that increase complexity and cost (e.g., foaming agents, dispensers with spray or foam actuators, oils, etc.) while providing a release agent in an effective quantity to allow efficient biocide loading and effective biocide recovery from a substrate for sterilization. Examples All equipment was cleaned and disinfected before batch processing. Appropriately sized beakers and propeller blades were selected based on the final test volumes. All ingredients listed in Tables 1 and 2 were added while mixing with the propeller blade at approximately 200 revolutions per minute (rpm). After the fragrance was added, mixing continued until the solution was uniform. Citric acid was added while mixing, and the pH was checked to an acidic range of approximately 4.5 to approximately 1. 5.0. Table 1 Component (INCI) Function % by weight Main Ingredients Benzalkonium Chloride (and) Ethanol (and) Water Biocide 0.01-0.5 PEG-8 Dimethicone Linoeamidopropyl PG-Dimodium, Silicone Quaternium-20 (and) Polypropylene Glycol, Quaternary Polydimethylsiloxane, PEG-5 Linoleammonium Dihydroxypropyl Chloride, Cocamidopropyl PG-Dimodium Chloride Phosphate, PEG-5 Linoleammonium Dihydroxypropyl Chloride and Behentrimonium Chloride, Cocamidopropyl PG-Dimonium Chloride Phosphate and Disodium Cocoamphodiacetate and / or Behentrimonium Chloride Release Agent 0.1-1.0 Other Ingredients Potassium Sorbate, Caprylyl Glycol, Disodium EDTA (and) Water, Aloe Barbadensis Leaf Juice (and) Water, PEG-8 Dimethicone, Fragrance and / or Citric Acid Preservative System, Chelating Agent, Extract, Emollient, 0.01-3.0 Water, Ethanol (and) Water Perfume, pH Adjuster Solvents QS to 100 Table 2 Component (INCI) Function % by weight Main Ingredients Benzethonium Chloride Biocide 0.01-0.5 PEG-8 Dimethicone Linamidopropyl PG-Dimodium, Silicone Quaternium-20 (and) Polypropylene Glycol, Quaternary Polydimethylsiloxane, Dihydroxypropyl PEG-5 Linolamonium Chloride, Cocamidopropyl PG-Dimonium Chloride Phosphate, Dihydroxypropyl PEG-5 Linolamonium Chloride and Behentrimonium Chloride, Cocamidopropyl PG-Dimonium Chloride Phosphate and Disodium Cocoamphodiacetate and / or Behentrimonium Chloride Release Agent 0.1-1.0 Other Ingredients Potassium Sorbate, Caprylyl Glycol, Phenoxyethanol (and) Methylparaben (and) Ethylparaben (and) Propylparaben, Caprylamphopropionate Sodium, PEG-60 Lanolin (and) Water, Disodium EDTA (and) Water, Aloe Barbadensis Leaf Juice (and) Water, PEG-8 Dimethicone, Fragrance and / or Citric Acid, Preservative System, Cleaning System, Chelating Agent, Extract, Emollient, Perfume, pH Adjuster 0.001-3.0 Water, Ethanol (and) Water, Solvents Q.S. to 100 With reference to Figure 1, Examples 1–10 were evaluated for the effect of certain release agents on the recovery of benzalkonium chloride from nonwoven wipes. The concentrations within the listed ranges of all ingredients in Table 1 were the same among Examples 1–10, except that the solvent was adjusted to account for a lack of release agent in Example 1. The type of release agent in the formulations varied among Examples 2–10. Additionally, Examples 2–7 and 10 had one release agent in their formulations, and Examples 8–9 had two release agents. The two release agents in Examples 8–9 were presented in equal parts and totaled the concentration of any single release agent in Examples 2–7 or 10, so that the total release agent concentration was the same in the formulations of Examples 2–10. The expressed fluid from a wipe was recovered by manually expressing the germicidal composition of saturated wipes in an appropriately sized beaker. After manual expression, the samples were mixed, and a portion of each sample was transferred to an appropriately sized sampler. Quantitative determination of biocide in the antibacterial formulations was performed using high-performance liquid chromatography (HPLC). At least five consecutive injections of the filtered standard solution into the HPLC system were used for suitability and calibration. Samples were prepared in duplicate, and each filtered solution was injected at least once into the HPLC system. Example 1 had no release agent and provided a 33.0% recovery of Benzalkonium Chloride in the expressed fluid of the wipes. Thus, 33.0% of the Benzalkonium Chloride in the germicidal composition poured onto the fibrous substrate to create the wipe (e.g., bulk solution) was recovered in the expressed fluid of the wipe. Example 2 had Hydroxypropyl BisHydroxyethyldimonium Chloride (not shown in Table 1) as a release agent and provided no appreciable effect on the recovery of Benzalkonium Chloride (33.2%). The QAC wetting agent, therefore, did not provide a suitable synergistic effect as a release agent.The recovery of Benzalkonium Chloride in the expressed fluid from the wipes, however, was unexpectedly modulated when the release agent was selected from a cationic quaternary ammonium halide surfactant not substituted with benzyl, a cationic quaternary ammonium silicone not substituted with benzyl, and / or a cationic quaternary ammonium phospholipid not substituted with benzyl. FIG. 1 shows the unexpected synergy of Benzalkonium Chloride with certain release agents listed in Table 1 in biocide recovery (and biocide efficacy) from wipes as follows: example 3, PEG-8 Dimethicone Linoamidopropyl PG-Dimodium (46.8%); example 4, Silicone Quaternium-20 (and) Polypropylene Glycol (52.4%); example b, Quaternary Polydimethylsiloxane (52.8%); example 6, Dihydroxypropyl PEG-5 Linolammonium Chloride (56.8%); example 7, Cocamidopropyl PG-Dimonium Chloride Phosphate (57.6%); example 8, Dihydroxypropyl PEG-5 Linolammonium Chloride and Behentrimonium Chloride (59.2%); Example 9, Cocamidopropyl PG-Dimonium Chloride Phosphate and Disodium Cocoamphodiacetate (60.8%); Example 10, Behentrimonium Chloride (69.4%). Table 3 shows that the recovery of benzalkonium chloride in the expressed wipe fluid can be modulated by varying levels of biocide in the germicidal composition at constant levels of release agent. Examples 10–11 in Table 3 were similar to Example 6, and Examples 12–14 in Table 3 were similar to Example 9, except that the concentration of benzalkonium chloride in the formulations varied within the ranges listed in Table 1 for Examples 10–14, while the solvent was adjusted to account for the changing amounts of biocide. As shown in Table 3, the biocide efficacy can be tailored to constant levels of release agent by altering the biocide concentration in the germicidal compositions (e.g., bulk solution). Table 3 Example % by Weight of Benzalkonium Chloride % Recovery Irritation 10 0.20 56.0 Negligible, significant irritation ion no 11 0.35 56.5 Negligible, significant irritation ion no 12 0.18 57.7 Negligible, significant irritation ion no 13 0.37 62.7 Negligible, significant irritation ion no 14 0.52 70.6 Negligible, significant irritation ion no Safety for the formulations of the examples 10-14 were also evaluated using a cumulative irritation test that implemented a repeated insult patch test in human participants to identify the irritation potential of substances or formulations. Sodium lauryl sulfate (SLS) was used as the positive control and the Webril patch was used as the negative control. Irritation was determined as a function of the Irritation Index. Cumulative / Irritation Potential. As shown in Table 3, irritation is negligible or insignificant despite increasing the levels of Benzalkonium Chloride in Examples 10-14. Unexpectedly, the recovery of Chloride Benzalkonium chloride in the expressed wipe fluid can also be modulated with varying levels of release agent in the germicidal composition at constant biocide levels. Examples 15–16 in Table 4 were similar to Example 6, except that the benzalkonium chloride concentration in the formulations was first decreased (but still within the ranges listed in Table 1) for Examples 15–16, and then the amount of release agent in the formulations was increased from 0.5% in Example 15 to 1.5% in Example 16, while the solvent was adjusted to account for the changing amounts of release agent. As shown in Table 4, the recovery of benzalkonium chloride in the expressed wipe fluid surprisingly increased from 56.0% to 72.8% with increasing levels of release agent.Notably, this result also contradicted conventional expectations that increasing amounts of the release agent would reduce the amount of biocide in the fluid, based on changes in relative concentrations. Thus, biocide efficacy can be tailored to constant biocide levels by altering the concentration of at least the release agent in germicidal compositions (e.g., bulk solution). Modulating at least the release agent, and / or associated amounts, can also be a useful mechanism for providing effective amounts of biocide in sterilization products without using unnecessary quantities of biocide and / or while meeting any applicable regulatory requirements. Table 4 Example % by Weight of Release Agent % of Biocide Recovery 15 0.5 56.0 16 1.5 72.8 Examples 17–25 were evaluated for the effect of certain release agents on the recovery of benzethonium chloride from nonwoven wipes. The concentrations within the ranges for all ingredients in Table 2 were the same among Examples 17–25 shown in Table 5, except that the concentration of certain release agents in the formulations listed in Table 2 varied within the listed ranges, and the solvent was adjusted in the formulations to account for the changing amounts of the release agent. The concentration of benzethonium chloride in the formulations within the ranges listed in Table 2 was the same in Examples 17–25 and Comparative Example 26. Comparative Example 26 had a formulation similar to commercially available nonwoven wipes under the brand name Wet Ones®, which lacks release agents. Table 5 Release Agent Example % by Weight of Release Agent % by Weight of Benzethonium Chloride % of Recovery Dihydroxypropyl Chloride PEG-5 Linolammonium 17 0.25 0.3 48.33 18 0.40 0.3 52.33 19* 0.50 0.3 53. 84 2 0 0 . 60 0.3 56.67 Cetrimonium Chloride 21 0.25 0.3 51.33 22 + 0.40 0.3 55.67 23 0.50 0.3 56.11 Behentrimonium Chloride 24 0.50 0.3 74.33 Cocamidopropyl PG-Dimonium Chloride Phosphate 25 0.50 0.3 54.00 None 2 6 0.0 0.3 42.83 (*) indicates that the formula was tested in duplicate and the recovery rate was reported as an average, (+) indicates that the formula was tested in triplicate and the recovery rate was reported as an average. As shown in Table 5, the release agents in the formulations of Examples 17–25 surprisingly increased the recovery of benzethonium chloride compared to Example 26, which had the same biocide concentration but lacked any release agent. Unexpectedly, the recovery of benzethonium chloride in the expressed wipe fluid was also modulated by varying levels of release agent in the germicidal composition at constant biocide levels. In this way, the biocide efficacy can be tailored to constant biocide levels by altering the concentration of the release agent in the germicidal compositions (e.g., bulk solution). Returning now to Figures 2A-2B, the in vitro extermination efficacy of germicidal solutions containing benzalkonium chloride was evaluated using a test based on the ASTM Standard Test Method for Estimation of Antimicrobial Activity for Water-Miscible Compounds Using a Time-Based Extermination Procedure (ASTM E2783-11 (2016)). The test product (antibacterial solution expressed from the wipes) was evaluated against a positive control (ethanol) and a placebo (saline solution). During the test, the product, positive control, and placebo were challenged with suspensions of 31 bacterial species. The percent and logio reduction of the initial population of each challenge species were determined after exposure of each test material. As shown in FIG. 2A, samples 11-14 provided a greater than 6-log extermination rate against Staphylococcus aureus (ATCC #6538, ATCC #29213) after thirty (30) seconds. As shown in FIG. 2B, samples 11-14 provided a greater than 6-log extermination rate against Escherichia coli (ATCC #11775, ATCC #25922) after 30 seconds. Thus, the modalities can provide a reduction greater than 99.9999%.The methods were additionally evaluated and found to be effective against: Human Coronavirus (alphacoronavirus), Bacteroides fragilis (ATCC #25285), Campylobacter jejuni (ATCC #33291 and ATCC #49943), Enterobacter cloacae (ATCC #13047), Enterococcus faecalis (ATCC #19433, ATCC #29212), Haemophilus influenzas (ATCC #19418), Klebsiella pneumonías (ATCC #13883), Pseudomonas aeruginosa (ATCC #15442, ATCC #27853), Salmonella Enteritidis (ATCC #13076), Salmonella enterica Typhimurium (ATCC #14028), Serralia marcescens (ATCC #8100), Shigella sonnei (ATCC #9290, ATCC #25931), Staphylococcus aureus MRSA (ATCC #33591, ATCC #33592), Streptococcus pneumoniae (ATCC #6303, ATCC #49619), Streptococcus pyogenes (ATCC #14289, ATCC #19615), Klebsiella pneumoniae (ATCC #27736), Serratia marcescens (ATCC #14756), Burkholderia cepacio (ATCC #25416, ATCC #25608), Listeria monocytogenes (ATCC #7644, ATCC #19115). Notas Adicionales y Ejemplos Additional Example 1 includes the germicidal composition comprising a biocide selected from the group consisting of a benzyl-substituted quaternary ammonium compound (QAC) and a release agent selected from the group consisting of a non-benzyl-substituted quaternary ammonium cationic surfactant, a non-benzyl-substituted quaternary ammonium cationic silicone, a non-benzyl-substituted quaternary ammonium cationic phospholipid, or any combination thereof. Additional Example 2 includes the germicidal composition of Additional Example 1, wherein the ratio of the release agent to the biocide is approximately 0.5:1 to approximately 3:1. Additional Example 3 includes the germicidal composition of any of Additional Examples 1-2, wherein the biocide is present in an amount of approximately 0.01% by weight to approximately 0.5% by weight, based on the total weight of the germicidal composition. Additional Example 4 includes the germicidal composition of any of Additional Examples 1-3, wherein the release agent is present in an amount of approximately 0.1% by weight to approximately 1.0% by weight, based on the total weight of the germicidal composition. Additional Example 5 includes the germicidal composition of any of Additional Examples 1-4, wherein the release agent is present in an amount of approximately 0.2% by weight to approximately 0.6% by weight and the biocide is present in an amount of approximately 0.1% by weight to approximately 0.4% by weight, based on the total weight 5 of the germicidal composition. Additional Example 6 includes the germicidal composition of any of Additional Examples 1-5, wherein the benzyl-substituted QAC is selected from the group consisting of alkyl dimethyl benzyl ammonium chloride, 10-diisobutylphenoxyethioxyethyl dimethyl benzyl ammonium chloride, or any combination thereof. Additional Example 7 includes the germicidal composition of any of Additional Examples 1-6, wherein the benzyl-substituted QAC includes Cs-Cie alkyl dimethyl benzyl 15 ammonium chloride. Additional Example 8 includes the germicidal composition of any of Additional Examples 1-7, wherein the non-benzyl-substituted QAC includes PEG-5 Linolammonium Dihydroxypropyl Chloride. Additional Example 9 includes the germicidal composition of any of Additional Examples 1-8, wherein the release agent further includes an amphoteric amine surfactant. Additional Example 10 includes the germicidal composition 25 of any of Additional Examples 1-9, and further includes a solvent, a preservative, an emollient, a preservative booster, a pH adjuster, a fragrance, a chelating agent, an extract, or any combination thereof. Additional Example 11 includes a wipe comprising a fibrous substrate and at least one biocide of a germicidal composition impregnated in the fibrous substrate, wherein the germicidal composition includes a biocide selected from the group consisting of a benzyl-substituted quaternary ammonium compound (QAC) and a release agent selected from the group consisting of a non-benzyl-substituted quaternary ammonium cationic surfactant, a non-benzyl-substituted quaternary ammonium cationic silicone, a non-benzyl-substituted quaternary ammonium cationic phospholipid, or any combination thereof. Additional Example 12 includes the wipe from Additional Example 11, wherein an initial ratio of the release agent to the biocide is approximately 0.5:1 to approximately 3:1. Additional Example 13 includes the wipe of any of Additional Examples 11-12, wherein the recovery of the biocide in the expressed fluid from the fibrous substrate will be modulated as the concentration of the releasing agent in the germicidal composition is modulated. Additional Example 14 includes the wipe of any of Additional Examples 11-13, wherein the benzyl-substituted QAC > ω N C N N CC «L U 42 is selected from the group consisting of alkyl dimethyl benzyl ammonium chloride, diisobutylphenoxyethoxyethyl dimethyl benzyl ammonium chloride, or any combination thereof. Additional Example 15 includes the wipe of any of Additional Examples 11-14, wherein the benzyl-substituted QAC includes Cs-Cis alkyl dimethyl benzyl ammonium chloride. Additional Example 16 includes the wipe of any of Additional Examples 11-15, wherein the non-benzyl-substituted QAC includes PEG-5 dihydroxypropyl linolammonium chloride. Additional Example 17 includes the wipe of any of Additional Examples 11-16, wherein the release agent further includes an amphoteric amine surfactant. Additional Example 18 includes the wipe of any of Additional Examples 11-17, plus it includes a solvent, a preservative, an emollient, a preservative booster, a pH adjuster, a fragrance, a chelating agent, an extract, or any combination thereof. Additional Example 19 includes the wipe from any of Additional Examples 11-18, and also includes a container within which the wipe is located. Additional Example 20 includes a method for sterilizing a surface comprising bringing the surface into contact with the wipe of any of Additional Examples 11-19. Additional Example 21 includes a non-foaming sterilization product comprising an anionic substrate and a germicidal composition in contact with the anionic substrate, wherein the germicidal composition includes a benzyl-substituted quaternary ammonium compound (QAC) including alkyl dimethyl benzyl ammonium chloride, diisobutylphenoxyethioxyethyl dimethyl benzyl ammonium chloride, or any combination thereof, a release agent including dihydroxypropyl PEG-5 linolammonium chloride present in an amount of approximately 0.2% by weight to approximately 0.75% by weight, based on the total weight of the germicidal composition, and a solvent. Additional Example 22 includes the sterilization product of Additional Example 21, wherein the ratio of the release agent to the biocide is approximately 0.5:1 to approximately 3:1. Additional Example 23 includes the sterilization product of any of Additional Examples 21-22, wherein the biocide is present in an amount of approximately 0.01% by weight to approximately 0.5% by weight, based on the total weight of the germicidal composition. Additional Example 24 includes the sterilization product of any of Additional Examples 21-23, wherein a total amount of the releasing agents in the germicidal composition is approximately 0.2% by weight to approximately 1.0% by weight, based on the total weight of the germicidal composition, and the biocide is present in an amount of approximately 0.1% by weight to approximately 0.4% by weight, based on the total weight of the germicidal composition. Additional Example 25 includes the sterilization product of any of Additional Examples 21-24, wherein the benzyl-substituted QAC includes Cg-Cie alkyl dimethyl benzyl ammonium chloride. Additional Example 26 includes the sterilization product of any of Additional Examples 21-25, wherein the release agent further includes behentrimonium chloride, centrimonium chloride, dicetyldimonium chloride, bishydroxyethyl dihydroxypropyl stearammonium chloride, or any combination thereof. Additional Example 27 includes the sterilization product of any of Additional Examples 21-26, wherein the germicidal composition further includes an amine amphoteric surfactant, a solvent, a preservative, an emollient, a preservative booster, a pH adjuster, a fragrance, a chelating agent, an extract, or any combination thereof. Additional Example 28 includes the sterilization product of any of Additional Examples 21-27, wherein the anionic substrate comprises a non-woven wipe. Additional Example 29 includes the sterilization product from any of Additional Examples 21-28, and also includes a container within which the sterilization product is located. Additional Example 30 includes a method for sterilizing a surface comprising bringing the surface into contact with the sterilizing product of any of Additional Examples 21-29. Additional Example 31 includes a method for preparing a sterilization product comprising selecting a substrate, contacting the substrate with a germicidal composition to impregnate the substrate with at least one biocide, wherein the germicidal composition includes a biocide selected from a benzyl-substituted quaternary ammonium compound and a release agent selected from a non-benzyl-substituted quaternary ammonium cationic surfactant, a non-benzyl-substituted quaternary ammonium cationic silicone, a non-benzyl-substituted quaternary ammonium cationic phospholipid, or any combination thereof, wherein the sterilization product is optionally non-foaming and / or excludes a foaming agent. Additional Example 32 includes the method from Example > N C N N Additional 31, further comprising selecting a substrate from the group consisting of an anionic substrate, contacting the substrate with the germicidal composition to impregnate the substrate with at least the biocide, wherein the germicidal composition includes a biocide selected from a benzyl-substituted quaternary ammonium compound (QAC) including alkyl dimethyl benzyl ammonium chloride, diisobutylphenoxyethioxyethyl dimethyl benzyl ammonium chloride, or any combination thereof, a release agent including dihydroxypropyl PEG-5 linolammonium chloride present in an amount of approximately 0.2% by weight to approximately 0.75% by weight, based on the total weight of the germicidal composition, and a solvent. Additional Example 33 includes the method of any of Additional Examples 31-32, furthermore including determining a final amount of the germicidal composition in the sterilization product based on the recovery of the biocide in the expressed fluid from the determined substrate from an increase in the amount of the release agent to increase the recovery of the biocide in the expressed fluid or a decrease in the amount of the release agent to decrease the recovery of the biocide in the expressed fluid. Additional Example 34 includes the method of any of Additional Examples 31-33, wherein the substrate comprises a non-woven wipe. Additional Example 35 includes the method of any of Additional Examples 31-34, in addition to comprising providing a container within which the sterilization product is located. Additional Example 36 includes the method of any of Additional Examples 31-35, wherein the container includes one or more of a can or bag. Additional Example 37 includes the method of any of Additional Examples 31-36, wherein the ratio of the release agent to the biocide is approximately 0.5:1 to approximately 3:1. Additional Example 38 includes the method of any of Additional Examples 31-37, wherein the biocide is present in an amount of approximately 0.01% by weight to approximately 0.5% by weight, based on the total weight of the germicidal composition. Additional Example 39 includes the method of any of Additional Examples 31-38, wherein a total amount of release agents in the germicidal composition is approximately 0.2% by weight to approximately 1.0% by weight, based on the total weight of the germicidal composition, and the biocide is present in an amount of approximately 0.1% by weight to approximately 0.4% by weight, based on the total weight of the germicidal composition. Additional Example 40 includes the method of any of Additional Examples 31-39, wherein the benzyl-substituted QAC includes Cs-Cis alkyl dimethyl benzyl ammonium chloride. Additional Example 41 includes the method of any of Additional Examples 31-40, wherein the release agent further includes behentrimonium chloride, centrimonium chloride, dicetyldimonium chloride, bishydroxyethyldihydroxypropyl stearammonium chloride, or any combination thereof. Additional Example 42 includes the method of any of Additional Examples 31-41, wherein the germicidal composition further includes an amine amphoteric surfactant, a solvent, a preservative, an emollient, a preservative booster, a pH adjuster, a fragrance, a chelating agent, an extract, or any combination thereof. While the present description has been particularly detailed in conjunction with specific preferred embodiments, it is evident that many alternatives, modifications, and variations will be apparent to those skilled in the art in view of the foregoing description. It is therefore contemplated that the appended claims will encompass any such alternatives, modifications, and variations as they fall within the true scope and spirit of the present description.

Claims

1. A non-foaming sterilization product, characterized in that it comprises: an anionic substrate; and a germicidal composition in contact with the anionic substrate, wherein the germicidal composition includes: a benzyl-substituted quaternary ammonium compound (QAC) including alkyl dimethyl benzyl ammonium chloride, diisobutylphenoxyethioxyethyl dimethyl benzyl ammonium chloride, or any combination thereof; a release agent including dihydroxypropyl PEG-5 linolammonium chloride present in an amount of approximately 0.2% by weight to approximately 0.75% by weight, based on the total weight of the germicidal composition; and a solvent.

2. The sterilization product according to claim 1, characterized in that the ratio of the release agent to the biocide is approximately 0.5:1 to approximately 3:

1.

3. The sterilization product according to claim 1, characterized in that the biocide is present in an amount of approximately 0.01% by weight to approximately 0.5% by weight, based on the total weight of the germicidal composition.

4. The sterilization product according to claim 1, characterized in that a total amount of release agent in the germicidal composition is approximately 0.2% by weight to approximately 1.0% by weight, based on the total weight of the germicidal composition, and the biocide is present in an amount of approximately 0.1% by weight to approximately 0.4% by weight, based on the total weight of the germicidal composition.

5. The sterilization product according to claim 1, characterized in that the benzyl-substituted QAC includes CgC18 alkyl dimethyl benzyl ammonium chloride.

6. The sterilization product according to claim 1, characterized in that the release agent further includes behentrimonium chloride, centrimonium chloride, dicetyldimonium chloride, bishydroxyethyl dihydroxypropyl stearammonium chloride, or any combination thereof.

7. The sterilization product according to claim 1, characterized in that the germicidal composition further includes an amphoteric amine surfactant, a solvent, a preservative, an emollient, a preservative booster, a pH adjuster, a fragrance, a chelating agent, an extract, or any combination thereof.

8. The sterilization product according to claim 1, characterized in that the anionic substrate > ω Ν C Ν Ν CC «Λ U 51 comprises a non-woven wipe.

9. The sterilization product according to claim 1, characterized in that it further includes a container within which the sterilization product is located.

10. A method for sterilizing a surface, characterized in that it comprises bringing the surface into contact with the sterilizing product of claim 1.

11. A method for preparing a non-foaming sterilization product, characterized in that it comprises: selecting a substrate from the group consisting of an anionic substrate; contacting the substrate with a germicidal composition to impregnate the anionic substrate with at least one biocide, wherein the germicidal composition includes: a benzyl-substituted quaternary ammonium compound (QAC) as the biocide, which includes alkyl dimethyl benzyl ammonium chloride, diisobutylphenoxyethioxyethyl dimethyl benzyl ammonium chloride, or any combination thereof; a release agent, which includes dihydroxypropyl PEG-5 linolammonium chloride present in an amount of approximately 0.2% by weight to approximately 0.75% by weight, based on the total weight of the germicidal composition; and a solvent.

12. The method according to claim 11, characterized in that it further includes determining a final amount of the germicidal composition in the sterilization product based on the recovery of the biocide in the expressed fluid from the determined anionic substrate from an increase in the amount of the release agent to increase the recovery of the biocide in the expressed fluid or a decrease in the amount of the release agent to decrease the recovery of the biocide in the expressed fluid.

13. The method according to claim 11, characterized in that the anionic substrate comprises a non-woven wipe.

14. The method according to claim 11, characterized in that it further comprises providing a container within which the sterilizing product is located.

15. The method according to claim 14, characterized in that the container includes one or more of a can or bag.

16. The method according to claim 11, characterized in that the ratio of the release agent to the biocide is approximately 0.5:1 to approximately 3:

1.

17. The method according to claim 11, characterized in that the biocide is present in an amount of approximately 0.01% by weight to approximately 0.5% by weight, based on the total weight of the germicidal composition.

18. The method according to claim 11, characterized in that the total amount of release agent in the germicidal composition is approximately 0.2% by weight to approximately 1.0% by weight, based on the total weight of the germicidal composition, and the biocide is present in an amount of approximately 0.1% by weight to approximately 0.4% by weight, based on the total weight of the germicidal composition. 10 19. The method according to claim 11, characterized in that the benzyl-substituted QAC includes Cs-Cis-alkyl dimethyl benzyl ammonium chloride 20. The method according to claim 11, characterized in that the release agent further includes 15 behentrimonium chloride, centrimonium chloride, dicetyldimonium chloride, bishydroxyethyl dihydroxypropyl stearammonium chloride, or any combination thereof.