Method for detecting pathogenic Escherichia coli bacteria expressing fimbria F17 using the CRISPR-Cas system and a set of LAMP primers.

Abstract

Method for detecting pathogenic strains of Escherichia coli expressing the f17g gene of the F17 fimbria gene. The invention relates to a detection method based on the amplification of the genomic DNA of the pathogenic strain using LAMP technology with specific primers at a temperature of 62°C. This reaction is carried out with the Cas12a enzyme, previously activated with a guide RNA specific to the F17g gene. This method allows for the rapid detection of pathogenic strains with rudimentary equipment and can be associated with a rapid diagnostic device in the field.