Oral composition containing herbal ingredients as active ingredients and bad breath remover containing same

Abstract

The present invention relates to an oral composition comprising herbal ingredients as active ingredients and a bad breath remover comprising same and, more specifically, to: an oral composition comprising, as active ingredients, Pinus densiflora Gnarl, Reynoutria japonica, and Rheum palmatum; and a bad breath remover comprising same. The present invention can provide: an oral composition which, by comprising herbal ingredients of Pinus densiflora Gnarl, Reynoutria japonica, and Rheum palmatum, has no toxicity to the human body and can inhibit the proliferation of periodontal bacteria, which is a fundamental cause of bad breath; and a bad breath remover comprising same.

Description

Oral composition containing herbal ingredients as active ingredients and breath freshener containing the same

[0001] The present invention relates to an oral composition comprising a herbal ingredient as an active ingredient and a breath freshener comprising the same, and more specifically, to an oral composition comprising pine needles, Korean knotweed, and rhubarb as active ingredients and a breath freshener comprising the same.

[0002] Halitosis is a foul odor detected in the oral cavity, which interferes with social activities such as interpersonal relationships. While halitosis can be caused by a variety of factors, including gum disease, dental caries, and gastrointestinal diseases, the most common and significant causes are gum disease and tongue coating. The volatile sulfur compounds produced by oral bacteria in gum disease and tongue coating as they break down oral proteins are the primary contributor to bad breath. Therefore, eliminating the oral bacteria that produce these volatile sulfur compounds is the most effective way to eliminate bad breath.

[0003] Traditionally, mouthwashes and other mouthwashes have been used primarily to combat bad breath. However, these contain alcohol, which can lead to complex problems like dry mouth. Listrin, a representative mouthwash, is known to be highly effective in suppressing bad breath, but its alcohol content is known to be over 10%. Furthermore, conventional mouthwashes and other mouthwashes fail to address the root cause of bad breath, as they do not address the antibacterial activity against periodontal bacteria, the root cause of bad breath.

[0004] Accordingly, there is a growing need for oral hygiene products that are non-toxic to the human body as they do not contain chemical ingredients such as alcohol, and oral hygiene products that can suppress the growth of periodontal bacteria, the fundamental cause of bad breath.

[0005] Additionally, with the recent rise in interest in pets, concern about their bad breath is also growing. Consequently, the need for oral hygiene products that can be used on pets who struggle to brush their teeth is growing.

[0006] The purpose of the present invention is to provide a non-toxic oral composition for humans, including medicinal ingredients of pine needles, rhizomes, and rhubarb, and a breath freshener containing the same.

[0007] Another object of the present invention is to provide an oral composition capable of inhibiting the proliferation of periodontal bacteria, which is the fundamental cause of bad breath, and a bad breath remover comprising the same.

[0008] Another object of the present invention is to provide an oral composition that can be used for pets and a breath freshener containing the same.

[0009] The technical problems to be solved by the invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by a person having ordinary skill in the art from the description of the present invention.

[0010] The present invention provides an oral composition comprising pine needles, rhizomes, and rhubarb as active ingredients.

[0011] In the present invention, the oral composition is characterized in that it inhibits at least one type of bacteria selected from the group consisting of Porphyromonas gingivalis and Fusobacterium nucleatum.

[0012] In the present invention, the oral composition is characterized in that it is for removing bad breath; and for preventing, improving, or treating periodontal disease.

[0013] In the present invention, the oral composition is characterized in that it is formulated as one or more types of formulations selected from the group consisting of toothpaste, mouthwash, mouthwash, gum, candy, mouth spray, oral ointment, and mouthwash.

[0014] In the present invention, it is characterized in that the pine needles, rhizome, and rhubarb are included in a weight ratio of 1:1:1.

[0015] In the present invention, the extract is characterized in that the pine needles, rhizomes, and rhubarb are each mixed in 95.0% ethyl alcohol and ultrasonic extraction is performed at 30 to 50 Kz and 1400 to 1600 W for 10 to 20 minutes.

[0016] In the present invention, the periodontal disease is characterized by being at least one type of periodontal disease selected from the group consisting of early periodontitis, moderate periodontitis, severe periodontitis, necrotizing periodontitis, and periodontal abscess.

[0017] In the present invention, the oral composition is characterized in that it further includes a filler.

[0018] In addition, the present invention provides a breath freshener containing pine needles, rhizomes, and rhubarb.

[0019] In the present invention, the breath freshener is characterized in that it is formulated as one or more types of formulations selected from the group consisting of toothpaste, mouthwash, mouth freshener, gum, candy, mouth spray, oral ointment, and mouthwash.

[0020] In the present invention, the breath freshener is characterized in that it further comprises calcium chloride hydrate, sodium carmoxyl methyl cellulose, potassium hydrogen monohydrate, D-sorbitol, potassium chloride, sodium chloride, and zinc.

[0021] In the present invention, the bad breath remover is characterized by including 2 parts by weight of pine needles, 2 parts by weight of Korean knotweed, 2 parts by weight of rhubarb, 140 to 160 parts by weight of calcium chloride hydrate, 9000 to 11000 parts by weight of sodium carmoxymethylcellulose, 320 to 360 parts by weight of potassium hydrogen monohydrate, 20 to 40 parts by weight of D-sorbitol, 1100 to 1300 parts by weight of potassium chloride, 830 to 850 parts by weight of sodium chloride, and zinc.

[0022] In the present invention, the breath freshener is characterized in that it further includes a pen.

[0023] In addition, the present invention provides an oral composition for a pet comprising the oral composition described above.

[0024] In addition, the present invention provides a breath freshener for pets, comprising the breath freshener described above.

[0025] The present invention can provide an oral composition that is non-toxic to the human body, including medicinal ingredients of pine needles, hojanggeun, and rhubarb, and a breath freshener containing the same.

[0026] In addition, the present invention can provide an oral composition capable of inhibiting the proliferation of periodontal bacteria, which is the fundamental cause of bad breath, and a bad breath remover comprising the same.

[0027] In addition, the present invention can provide an oral composition that can be used for pets and a breath freshener containing the same.

[0028] The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

[0029] Figure 1 is a schematic diagram showing the experimental steps for selecting 4 types of herbal ingredients with excellent bad breath suppression properties among 252 types of herbal ingredients.

[0030] Figure 2 is a graph showing the results of analyzing the periodontal bacteria growth inhibition ability of 252 herbal ingredients.

[0031] Figure 3 is a graph showing the results of analyzing the antibacterial activity of 252 herbal ingredients against periodontal bacteria.

[0032] Figure 4 is a graph showing the results of analyzing the cytotoxicity of 252 herbal ingredients.

[0033] Figure 5 is a graph showing the results of analyzing the bad breath suppression ability of 32 kinds of herbal ingredients selected in the first stage when used alone.

[0034] Figure 6 is a graph showing the results of analyzing the bad breath suppression ability of the four selected herbal ingredients when used alone.

[0035] Figure 7 is a graph showing the results of analyzing the bad breath suppression ability when the four finally selected herbal ingredients were combined in various combinations.

[0036] The terms used in this specification have been selected from widely used, current terms, taking into account the functions of the present invention. However, these terms may vary depending on the intentions of those skilled in the art, precedents, the emergence of new technologies, etc. Furthermore, in certain cases, terms may be arbitrarily selected by the applicant, and in such cases, their meanings will be described in detail in the relevant description of the invention. Therefore, the terms used in this invention should not be defined simply as names, but rather based on their inherent meanings and the overall content of the present invention.

[0037] Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with their meaning in the context of the relevant technology, and will not be interpreted in an idealized or overly formal sense unless explicitly defined herein.

[0038] Numerical ranges are inclusive of the numbers defined in the ranges above. Every maximum numerical limitation given throughout this specification includes every lower numerical limitation, as if that lower numerical limitation were explicitly stated. Every minimum numerical limitation given throughout this specification includes every higher numerical limitation, as if that higher numerical limitation were explicitly stated. Every numerical limitation given throughout this specification will include every better numerical range within that broader numerical range, as if that narrower numerical limitation were explicitly stated.

[0039]

[0040] Hereinafter, the present invention will be described in detail.

[0041]

[0042] Oral composition

[0043] The present invention can provide an oral composition comprising pine needles, rhizomes, and rhubarb as effective ingredients.

[0044] The above oral composition may be characterized by inhibiting at least one type of bacteria selected from the group consisting of Porphyromonas gingivalis and Fusobacterium nucleatum, and more specifically, may be characterized by having growth inhibition and antibacterial activity against at least one type of bacteria selected from the group consisting of Porphyromonas gingivalis and Fusobacterium nucleatum. Preferably, the oral composition may be characterized by inhibiting Porphyromonas gingivalis.

[0045] The oral composition above may have excellent safety for the body due to its low cytotoxicity by using a herbal ingredient as an active ingredient. The oral composition may be characterized as being for removing bad breath; and preventing, improving, or treating periodontal disease. The periodontal disease may refer to early periodontitis, moderate periodontitis, severe periodontitis, necrotizing periodontitis, or periodontal abscess, and preferably refers to moderate periodontitis, severe periodontitis, or periodontal abscess.

[0046] The above oral composition may be characterized in that it is formulated as one or more types of formulations selected from the group consisting of toothpaste, mouthwash, mouth freshener, gum, candy, mouth spray, oral ointment, and mouthwash, and preferably, it may be characterized in that it is formulated as an oral spray.

[0047] The pine needles, hollyhock root, and rhubarb contained in the oral composition may be characterized by being contained in a weight ratio of 1:1:1. If the weight ratio of the pine needles, hollyhock root, and rhubarb contained in the oral composition deviates from 1:1:1, the bad breath reduction and antibacterial effects may be reduced, and the adhesiveness may be reduced and the wettability may be undesirable.

[0048] The above oral composition may further include filbal, preferably pine needles, filbal, hojanggeun, and rhubarb in a weight ratio of 1:1:1:1.

[0049] The above oral composition may be characterized in that the extracts are prepared by mixing the pine needles, the Korean knotweed, and the rhubarb root in 95.0% ethyl alcohol, respectively, and performing ultrasonic extraction at 30 to 50 Kz and 1400 to 1600 W for 10 to 20 minutes, and preferably, the extracts are prepared by mixing the pine needles, the Korean knotweed, and the rhubarb root in 95.0% ethyl alcohol, respectively, and performing ultrasonic extraction at 40 Kz and 1500 W for 15 minutes.

[0050]

[0051] Bad breath remover

[0052] The present invention can provide a breath freshener containing pine needles, Korean knotweed, and rhubarb as active ingredients, and the breath freshener can be characterized in that it is formulated as one or more types of formulations selected from the group consisting of toothpaste, mouthwash, mouth freshener, gum, candy, mouth spray, mouth ointment, and mouthwash, and preferably, it can be characterized in that it is formulated as a mouth spray formulation.

[0053] The above-mentioned breath freshener may be characterized by further comprising calcium chloride hydrate, sodium carmoxymethylcellulose, potassium monobasic acid, D-sorbitol, potassium chloride, sodium chloride and zinc, and preferably comprises 2 parts by weight of pine needles, 2 parts by weight of Korean knotweed, 2 parts by weight of rhubarb, 140 to 160 parts by weight of calcium chloride hydrate, 9000 to 11000 parts by weight of sodium carmoxymethylcellulose, 320 to 360 parts by weight of potassium monobasic acid, 20 to 40 parts by weight of D-sorbitol, 1100 to 1300 parts by weight of potassium chloride, 830 to 850 parts by weight of sodium chloride and zinc, and more preferably comprises 2 μg / mL of pine needles, 2 μg / mL of Korean knotweed, 2 μg / mL of rhubarb, and calcium chloride hydrate. It can be characterized by containing 0.15 mg / mL of sodium carmoxymethylcellulose, 10 mg / mL of sodium carboxymethylcellulose, 0.34 mg / mL of potassium hydrogen monohydrate, 30 μg / mL of D-sorbitol, 1.2 mg / mL of potassium chloride, 0.84 mg / mL of sodium chloride and 1% zinc.

[0054] The above-mentioned breath freshener may further include pilbal, and preferably, based on 2 parts by weight of pine needles, 2 parts by weight of pilbal, 2 parts by weight of Korean knotweed, 2 parts by weight of rhubarb, 140 to 160 parts by weight of calcium chloride hydrate, 9000 to 11000 parts by weight of sodium carmoximethylcellulose, 320 to 360 parts by weight of potassium monobasic acid, 20 to 40 parts by weight of D-sorbitol, 1100 to 1300 parts by weight of potassium chloride, 830 to 850 parts by weight of sodium chloride, and zinc, and more preferably, 2 μg / mL of pine needles, 2 μg / mL of pilbal, 2 μg / mL of Korean knotweed, 2 μg / mL of rhubarb, 0.15 mg / mL of calcium chloride hydrate, 10 mg / mL of sodium carmoximethylcellulose, and potassium monobasic acid. It may be characterized by containing 0.34 mg / mL of sodium chloride, 30 μg / mL of D-sorbitol, 1.2 mg / mL of potassium chloride, 0.84 mg / mL of sodium chloride and 1% of zinc.

[0055]

[0056] In addition, the present invention can provide an oral composition for a pet including the oral composition described above, and can provide a breath freshener for a pet including the breath freshener described above.

[0057] Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited to the following examples.

[0058]

[0059] Experimental Example 1. Analysis of the ability of 252 herbal ingredients to inhibit the growth of periodontal bacteria.

[0060] 252 herbal ingredients were distributed from the Korea Plant Extract Bank (currently the Natural Products Central Bank of the Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of Korea), and the inhibitory activity against periodontal bacteria growth of the corresponding preparations was confirmed through absorbance measurement. The serial numbers, distribution numbers, and country / product names of the 252 herbal ingredients are listed in Table 1. All herbal ingredients used in the experiment were prepared using 95.0% ethyl alcohol, and the detailed extraction method is described in Experimental Example 5 below.

[0061]

[0062]

[0063]

[0064] Hereinafter, the absorbance measurement method is described in detail. First, GAM broth, a periodontal bacteria culture medium, was dispensed into each 96-well plate, and the same amount of periodontal bacteria (Porphyromonas gingivalis or Fusobacterium nucleatum) was applied, and then each of the 252 herbal medicines was added to prepare 252 samples. Next, the absorbance of the 252 samples before placing them in an anaerobic incubator was measured, and the absorbance after 24 hours of incubation in the incubator was measured to calculate the inhibition of periodontal bacteria growth. In order to correct for the difference in absorbance due to the herbal medicine, the absorbance of samples to which only the culture medium and each herbal medicine was applied without adding periodontal bacteria were also measured before and after incubation to establish a reference point.

[0065] The absorbance measured in the above Experimental Example 1 is shown graphically in Fig. 2. The absorbance of all 252 herbal preparations was measured, but only 32 herbal preparations listed in Table 2 below are shown in Fig. 2. The vertical axis of Fig. 2 represents the periodontal bacteria inhibition ability, and the degree of periodontal bacteria proliferation according to the presence or absence of herbal treatment was confirmed by measuring the absorbance, and the periodontal bacteria inhibition ability was calculated using the following Equation 1. More specifically, the lower the y value in Fig. 2, the better the periodontal bacteria inhibition ability.

[0066]

[0067] Serial number, distribution number, country name / product name 8CA01-085, lily 25CA03-010, windbreak 29CA03-030, turmeric 31CA03-064, branch berry 43CW03-093, tongcho 60CA01-018, gwijeonwoo 61CA01-020, tangerine kernel 77CA01-056, mihudo 87CA01-077, baekchuk 123CA02-053, seokgok 127CA02-058, seonbokhwa 153CA03-005, male (wooden dam) 161CA03-020, azalea (=linseed) 163CA03-023, wasong 165CA03-027, ubangja 169CA03-034, incense 170CA03-035 Chicken 172CA03-039 Leein (element) 179CA03-052 Carving 186CA03-066 Jiyu 192CA03-075 Chodugu 194CA03-080 Octagonal incense (= Daehwa incense) 200CA04-087 Gold 205CA03-097 Hojanggeun 207CA04-008 Rhubarb 210CA04-018 White jasmine 215CA04-028 Sangji 216CA04-029 Sesin (root) 217CA04-030 Songjeol 219CA04-034 Yugeunpi 228CA04-055 Cheongsangja 244CA04-079 Pilbal

[0068]

[0069] [Formula 1]

[0070]

[0071]

[0072] As a result of Experimental Example 1, the periodontal bacteria inhibition ability against P. gingivalis was excellent in many herbal medicine preparations, including Hojanggeun (serial number 205), Rheum amurense (serial number 207), and Songjeol (serial number 217), but the periodontal bacteria inhibition ability was not excellent in tangerine kernel (serial number 61) and Yugeunpi (serial number 219).

[0073] In addition, the periodontal bacteria inhibition activity against F. nucleatum was excellent in many herbal medicine preparations, including Hojanggeun (serial no. 205) and Songjeol (serial no. 217), but the periodontal bacteria inhibition activity was not excellent in broiler chicken (serial no. 170) and rhubarb (serial no. 207).

[0074]

[0075] Experimental Example 2. Analysis of the antibacterial activity of 252 herbal ingredients against periodontal bacteria.

[0076] The antibacterial activity of 252 herbal ingredients against periodontal bacteria was confirmed using the disk diffusion method. The disk diffusion method is described in detail below. First, agar was added to GAM broth, a periodontal bacteria culture medium, and heated to sterilize. After sufficient cooling, periodontal bacteria were mixed and poured into culture dishes to prepare culture dishes containing a mixture of periodontal bacteria.

[0077] Next, an antibacterial activity measurement disk with a thickness of 0.7 mm and a diameter of 60 mm was placed on the culture dish, and 10 μl of the herbal ingredient at a concentration of 100 mg / ml was applied to the disk. After culturing in an anaerobic incubator for 24 hours, the antibacterial activity against periodontal bacteria was confirmed by observing the zone of transparency around the disk. Metronidazole, a representative antibiotic against anaerobic bacteria, was used as a positive control, and the relative antibacterial effect was calculated based on the effect of metronidazole as 100%.

[0078] The results of the above Experimental Example 2 are shown graphically in Fig. 3. As with Fig. 2, the absorbance of all 252 herbal preparations was measured, but Fig. 3 only shows the 32 herbal preparations listed in Table 2.

[0079] As a result of Experimental Example 2, the antibacterial activity of Hojanggeun (serial number 205), Rhubarb (serial number 207), Sesin (serial number 216), and Songjeol (serial number 217) against Porphyromonas gingivalis was the best.

[0080]

[0081] Experimental Example 3. Cytotoxicity Analysis of 252 Herbal Ingredients

[0082] The cytotoxicity of 252 herbal ingredients was confirmed using the MTT assay. The method for evaluating cytotoxicity is described in detail below. 8,000 cells were seeded onto each culture plate, and after 16 hours, the herbal ingredients were treated and cultured for 24 hours. A tetrazolium dye was applied to the cells treated with the herbal ingredients, and the cells were cultured for an additional 4 hours. The supernatant was removed. The cells from which the supernatant was removed were treated with dimethyl sulfoxide to dissolve the formazan crystals produced by the chemical reaction. The absorbance at a wavelength of 570 nm was measured using a microplate spectrophotometer. Two cell lines were used to confirm toxicity: Ca9-22 cells, which correspond to gingival epithelial cells, and HaCaT cells, which are keratinized epithelial cells.

[0083] The results of the above Experimental Example 3 are shown graphically in Fig. 4. As with Figs. 2 and 3, the absorbance of all 252 herbal preparations was measured, but Fig. 4 only shows the 32 herbal preparations listed in Table 2.

[0084] As a result of Experimental Example 3, no cytotoxicity was observed in most herbal preparations including Hojanggeun (serial number 205), Rheum rhizome (serial number 207), Sesin (serial number 216), and Songjeol (serial number 217), but cytotoxicity was observed in Curcumin (serial number 29), Tangerine kernel (serial number 61), Baekchuk (serial number 87), Seonbokhwa (serial number 127), Yuhyang (serial number 169), Chodugu (serial number 192), Cheongsangja (serial number 228), and Pilbal (serial number 244).

[0085]

[0086] Experimental Example 4. Analysis of the Bad Breath Inhibition Effect of 32 Primary Selected Herbal Ingredients Used Single

[0087] Based on the results of Experimental Examples 1 to 3 above, 32 herbal ingredients with excellent periodontal bacteria inhibition and antibacterial activity and low cytotoxicity were first selected, and the 32 herbal ingredients are as described in Table 2 above. The halitosis inhibition activity of the 32 herbal ingredients when used alone was analyzed using P. gingivalis and F. nucleatum, which are the main periodontal bacteria that produce halitosis. To analyze the halitosis inhibition activity, the methyl mercaptan inhibition activity when the 32 herbal ingredients were used at a concentration of 200 ㎍ / ㎖ was analyzed as a percentage based on the amount of methyl mercaptan when the herbal ingredients were not treated. Briefly, periodontal bacteria were inoculated into the culture medium and cultured for 16 hours. The amount of methyl mercaptan was measured using a halitosis measuring device after 6 hours with or without applying the herbal ingredients. The inhibitory activity was expressed as a percentage of the amount of methyl mercaptan in the treated and untreated samples.

[0088] The results of the above Experimental Example 4 are shown graphically in Fig. 5. As with Figs. 2 to 4, the bad breath suppressing ability of all 252 herbal preparations was measured, but Fig. 5 only shows the 32 herbal preparations listed in Table 2.

[0089] As a result of Experimental Example 4, the halitosis suppression activity against P. gingivalis was excellent in Baekchuk (serial number 87), Yuhwang (serial number 169), Hojanggeun (serial number 205), Rheum (serial number 207), Songjeol (serial number 217), and Pilbal (serial number 244). The halitosis suppression activity against F. nucleatum was excellent in Jiyu (serial number 187) and Songjeol (serial number 217), and in particular, Songjeol's halitosis suppression activity against F. nucleatum was very excellent.

[0090]

[0091] Experimental Example 5. Analysis of the Bad Breath Control Effect of the Four Final Selected Herbal Ingredients Used Single

[0092] Based on the results of the above Experimental Example 4, four herbal ingredients (Pine needle, Pilbal, Hojanggeun, and Rhubarb) with excellent bad breath suppression properties were finally selected. The extraction method for each preparation was the same, but the initial application weight and final extract weight were different: 1) in the case of Pine needle (distribution number CA04-030), 31.12g was obtained by using 140g, 2) in the case of Pilbal (distribution number CA04-079), 7.54g was obtained by using 100g, 3) in the case of Hojanggeun (distribution number CA03-097), 5.85g was obtained by applying 80g, and 4) in the case of Rhubarb (distribution number CA04-008), 9.53g was obtained by using 74g.

[0093] The extraction method for each preparation is briefly described as follows. The shade-dried herbal medicine was ground into powder, mixed with 1 L of 95.0% ethyl alcohol, and then ultrasonic extraction was performed for 15 minutes at 40 Kz and 1500 W using an ultrasonic extractor (SDN-900H, SD-ULTRASONIC CO., LTD). The extraction was repeated 30 times (cycles) at room temperature. The extract (Qualitative Filter No. 100, HYUNDAI MICRO CO., LTD) was filtered and dried under reduced pressure. The bad breath inhibition ability of the four herbal ingredients mentioned above when used alone was analyzed in the same manner as in Experimental Example 4, and the herbal ingredients were used at a concentration of 20 μg / ml. The results of Experimental Example 5 are shown in a graph in Fig. 6.

[0094] As a result of Experimental Example 5, when the above-mentioned Songjeol, Pilbal, Hojanggeun, and Rhubarb were used alone, the bad breath suppression effect was not excellent. More specifically, when Songjeol was used alone, the bad breath suppression effect against P. gingivalis appeared to be somewhat excellent, but the degree was minimal, and when all ingredients were used alone, the bad breath suppression effect against F. nucleatum was not found.

[0095]

[0096] Experimental Example 6. Analysis of nitric oxide inhibition ability when the four selected herbal ingredients were used alone.

[0097] After selecting four herbal ingredients (Pine Tree, Pine Tree, Hojanggeun, and Rheum) with excellent halitosis suppressing properties, their anti-inflammatory effects were confirmed through nitric oxide suppression assay. First, Raw264.7 macrophages were cultured at 2×10 5 The cells were seeded in 24-well plates and cultured for 16 hours, and then treated with four herbal ingredients at a concentration of 50 μg / ml and cultured for an additional 24 hours. The supernatant of the cultured cells was used to determine changes in nitric oxide production using a NO assay kit (Abcam, USA). The nitric oxide production measurement experiment was conducted according to the experimental method in the kit, and after the reaction was completed, the absorbance was measured at a wavelength of 560 nm using a microplate spectrophotometer.

[0098] The results of the above experimental example 6 are shown in Table 3, and in Table 3, the degree to which nitric oxide (NO) is reduced by the herbal ingredient is expressed as a percentage, using the content of nitric oxide (NO) generated in the absence of the herbal ingredient as a reference point.

[0099]

[0100] Herbal preparation (applicable concentration: 50 ㎍ / ㎖) Nitric oxide production inhibition ability (%) Songjeol 22.7 Pilbal 82.1 Hojanggeun 40.41 Rheum 36.81

[0101]

[0102] As a result of Experimental Example 6, the nitric oxide inhibition abilities of Songjeol, Pilbal, Hojanggeun, and Rhubarb were 22.7, 82.1, 40.41, and 36.81%, respectively.

[0103]

[0104] Experimental Example 7. Analysis of the Bad Breath Suppression Effect of the Four Final Selected Herbal Ingredients Combined

[0105] The four selected herbal ingredients (pine needles, pine needles, Japanese knotweed, and rhubarb) were combined in various combinations and their halitosis-inhibiting properties were analyzed when used in combination. In Experimental Example 7, the following combinations were used in combination: (①) pine needles and pine needles; (②) pine needles and Japanese knotweed; (③) pine needles and rhubarb; (④) pine needles, pine needles, and Japanese knotweed; (⑤) pine needles, Japanese knotweed, and rhubarb; (⑥) pine needles, pine needles, and rhubarb.

[0106] The bad breath suppression ability was analyzed using the same method as in Experimental Example 4, and the herbal ingredients were used at a concentration of 10 or 5 μg / ml. The results of Experimental Example 7 are shown graphically in Fig. 7, and the 1:20 dilution represents the result using the herbal ingredients at a concentration of 10 μg / ml, and the 1:40 dilution represents the result using the herbal ingredients at a concentration of 5 μg / ml.

[0107] As a result of Experimental Example 7, (⑤) the halitosis suppression effect was the best when combined with a combination of pine needles, Korean knotweed, and rhubarb. More specifically, (⑤) the halitosis suppression effect against P. gingivalis was excellent when combined with a combination of pine needles, Korean knotweed, and rhubarb, but no halitosis suppression effect was observed against F. nucleatum.

[0108]

[0109] Example 1. Preparation of an oral composition containing pine needles, hollyhock root, and rhubarb

[0110] Example 1 was conducted to prepare an oral composition containing pine needles, Korean knotweed, and rhubarb. The pine needles, Korean knotweed, and rhubarb were each extracted in the same manner as the extraction method described in Experimental Example 5, thereby preparing extracts of pine needles, Korean knotweed, and rhubarb, respectively. The extracts of pine needles, Korean knotweed, and rhubarb were mixed in a weight ratio of 1:1:1 to prepare an oral composition.

[0111]

[0112] Example 2. Preparation of oral spray containing pine needles, rhizome, and rhubarb

[0113] An oral spray was prepared based on the oral composition of Example 1. The oral spray was prepared with the composition of calcium chloride hydrate 0.15 mg / mL, sodium carmoxymethylcellulose 10 mg / mL, potassium monobasic acid 0.34 mg / mL, D-sorbitol 30 μg / mL, potassium chloride 1.2 mg / mL, 1% zinc, sodium chloride 0.84 mg / mL, pine needle 2 μg / mL, Korean knotweed 2 μg / mL, and rhubarb 2 μg / mL.

[0114]

[0115] From the above description, those skilled in the art will understand that the present invention can be implemented in other specific forms without altering its technical spirit or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all respects and are not limiting.

Claims

1. An oral composition containing pine needles, hollyhock root, and rhubarb as effective ingredients.

2. In paragraph 1, The above oral composition An oral composition characterized in that it inhibits at least one type of bacteria selected from the group consisting of Porphyromonas gingivalis and Fusobacterium nucleatum.

3. In paragraph 1, The above oral composition An oral composition characterized by being for removing bad breath; and preventing, improving or treating periodontal disease.

4. In paragraph 1, The above oral composition An oral composition characterized in that it is formulated as one or more dosage forms selected from the group consisting of toothpaste, mouthwash, mouthwash, gum, candy, mouth spray, oral ointment, and mouthwash.

5. In paragraph 1, The above Songjeol, Hojanggeun and Daehwang are An oral composition characterized in that it is contained in a weight ratio of 1:1:

1.

6. In paragraph 1, The above Songjeol, Hojanggeun and Daehwang are An oral composition characterized in that the extract is manufactured by mixing each of the extracts in 95.0% ethyl alcohol and performing ultrasonic extraction at 30 to 50 Kz and 1400 to 1600 W for 10 to 20 minutes.

7. In paragraph 3, The above periodontal disease An oral composition characterized by being at least one periodontal disease selected from the group consisting of early periodontitis, moderate periodontitis, severe periodontitis, necrotizing periodontitis, and periodontal abscess.

8. In paragraph 1, The above oral composition An oral composition characterized by further comprising a pen.

9. A breath freshener containing pine needles, hollyhock root, and rhubarb as effective ingredients.

10. In paragraph 9, A breath freshener, characterized in that the above breath freshener further comprises a pen.

11. In paragraph 9, The above breath freshener A breath freshener characterized by being formulated as one or more dosage forms selected from the group consisting of toothpaste, mouthwash, mouthwash, gum, candy, mouth spray, oral ointment, and mouthwash.

12. In paragraph 9, The above breath freshener A breath freshener characterized by further comprising calcium chloride hydrate, sodium carmoxyl cellulose, potassium monobasic acid, D-sorbitol, potassium chloride, sodium chloride and zinc.

13. In paragraph 9, The above breath freshener A breath freshener, characterized in that it comprises 2 parts by weight of pine needles, 2 parts by weight of Korean knotweed, 2 parts by weight of rhubarb, 140 to 160 parts by weight of calcium chloride hydrate, 9000 to 11000 parts by weight of sodium carmoxamethylcellulose, 320 to 360 parts by weight of potassium hydrogen monohydrate, 20 to 40 parts by weight of D-sorbitol, 1100 to 1300 parts by weight of potassium chloride, 830 to 850 parts by weight of sodium chloride, and zinc.

14. An oral composition for a companion animal comprising an oral composition according to any one of claims 1 to 8.

15. A pet deodorant comprising a deodorant according to any one of clauses 9 to 13.

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