Skin care active substance and skin care product

Abstract

A skin care active substance and a skin care product. The skin care active substance comprises, in parts by weight, 0.1-15 parts of a moisturizing agent and 0.1-12 parts of a soothing agent, wherein the moisturizing agent comprises at least one of four-molecular-weight hyaluronic acid and D-panthenol, and the soothing agent comprises at least one of a Portulaca oleracea extract, dipotassium glycyrrhizinate, a Piper methysticum extract, and trans-4-tert-butylcyclohexanol. By designing the components and the ratio thereof, the synergistically formed active substance can mildly and effectively provide moisturizing, soothing, and repairing effects, thereby effectively protecting the skin barrier, maintaining skin health, and demonstrating high safety.

Description

Skin care actives, skin care products

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims priority based on Chinese patent application 202410107435.X filed on January 25, 2024, and all of its contents are incorporated herein by reference. Technical Field

[0003] The present application relates to the technical field of skin care, and in particular to skin care active ingredients and skin care products. Background Art

[0004] The skin is the body's first line of defense against external aggression. The skin barrier function maintains the normal operation of the skin's physiological functions by preventing the loss of water, nutrients, etc.

[0005] On the one hand, environmental factors such as strong sunlight, ultraviolet radiation from concentrated sunlight, air pollution from smog and dust, and dryness and excessive humidity caused by climate change can pose significant challenges to skin health, leading to symptoms such as itching, redness, swelling, and discomfort like dryness and tightness. On the other hand, the negative emotions brought on by high-intensity work and unhealthy lifestyles can lead to sub-healthy or even damaged skin barriers, making the skin more susceptible to dehydration, oil-water imbalance, and even allergic reactions such as redness, dryness, itching, stinging, and swelling.

[0006] Summary of the Invention

[0007] The present application provides a skin care active ingredient and skin care product, which has moisturizing, soothing and repairing effects, effectively protects the skin barrier and maintains skin health.

[0008] To solve the above technical problems, the first aspect of the present application provides a skin care active ingredient, which includes 0.1-15 parts by weight of a moisturizer and 0.1-12 parts by weight of a soothing agent in a weight ratio; wherein the moisturizer includes at least one of quadruple hyaluronic acid and D-panthenol, and the quadruple hyaluronic acid includes sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate; the soothing agent includes at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol.

[0009] In one embodiment, the skin care active ingredient comprises, by weight, 0.1-10 parts by weight of tetra-hyaluronic acid and / or 0.1-5 parts by weight of D-panthenol.

[0010] In one embodiment, the skin care active ingredient comprises, by weight, at least one of: 0.5-5 parts by weight of purslane extract, 0.5-5 parts by weight of dipotassium glycyrrhizate, 0.1-1 parts by weight of kava extract, and 0.1-1 parts by weight of trans-4-tert-butyl transhexanol.

[0011] In one embodiment, the skin care active ingredient further comprises 0.1-15 parts by weight of an anti-allergic agent, wherein the anti-allergic agent comprises at least one of a unicorn extract, a polygonatum extract, and a panax notoginseng extract.

[0012] In one embodiment, the skin care active is composed of the following components in the following weight ratios: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract; or

[0013] The skin care active ingredient is composed of the following components in the following weight ratio: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts by weight of D-panthenol, 0.5-5 parts by weight of purslane extract, and 0.1-1 parts of kava extract; or

[0014] The skin care active ingredient is composed of the following components in the following weight ratio: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts by weight of D-panthenol, 0.5-5 parts by weight of purslane extract, and 0.1-5 parts by weight of unicorn extract; or

[0015] The skin care active ingredient is composed of the following components in the following weight ratio: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts of D-panthenol, 0.1-1 parts of kava extract, and 0.1-5 parts of unicorn extract; or

[0016] The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-10 parts by weight of tetrahyaluronic acid, 0.5-5 parts by weight of purslane extract, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract; or

[0017] The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract; or

[0018] The skin care active ingredient is composed of the following components in the following weight ratios: 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, and 0.1-1 part of kava extract.

[0019] In one embodiment, the weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate in the quadruple hyaluronic acid is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

[0020] In order to solve the above technical problems, the second aspect of the present application provides a skin care product, comprising any of the skin care active ingredients described above.

[0021] In one embodiment, the skin care product comprises 0.2-42 parts by weight of the skin care active ingredient and 5.6-13 parts by weight of the base material.

[0022] In one embodiment, the skin care product consists of the skin care active, the base and deionized water.

[0023] In one embodiment, the base material is composed of the following components in the following weight ratios: 2-5 parts by weight of glycerin, 1-2 parts by weight of polydimethylsiloxane, 2-4 parts by weight of squalane, 0.1-0.5 parts by weight of acrylic acid or acrylate or C10-30 alkyl acrylate crosspolymer, and 0.5-1.5 parts by weight of polysorbate 60.

[0024] The beneficial effects of this application are as follows: Different from the prior art, this application discloses a skin care active ingredient and skin care product; the skin care active ingredient comprises, by weight, 0.1-15 parts by weight of a moisturizer and 0.1-12 parts by weight of a soothing agent; wherein the moisturizer comprises at least one of quadruple hyaluronic acid and D-panthenol, and the quadruple hyaluronic acid comprises sodium hyaluronate cross-polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate; the soothing agent comprises at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol. By designing the above ingredients and their proportions, the synergistically formed active ingredient can gently and efficiently produce moisturizing, soothing, and repairing effects, effectively protecting the skin barrier and maintaining skin health, with high safety.

[0025] The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0027] FIG1 is a comparison chart of cell proliferation activity test results;

[0028] Figure 2 is a comparison of cell scratches;

[0029] Figure 3 is a comparison chart of cell scratch test data;

[0030] Figure 4 is a comparison of mitochondrial membrane potential;

[0031] Figure 5 is a comparison diagram of mitochondrial membrane potential test data;

[0032] Figure 6 is a comparison chart of IL-1α, IL-6, and PGE2 test results;

[0033] Figure 7 is a comparison chart of TNF-α and TRPV1 test results. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solution and effect of this application clearer and more specific, the following embodiments of the technical solution of this application will be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of this application and are therefore only examples and are not intended to limit the scope of protection of this application.

[0035] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned figure descriptions are intended to cover non-exclusive inclusions.

[0036] In the description of the embodiments of the present application, the technical terms "first" and "second" are used only to distinguish different objects and should not be understood as indicating or implying relative importance or implicitly indicating the quantity, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of the present application, the term "multiple" refers to more than two (including two), similarly, "multiple groups" refers to more than two (including two), and "multiple pieces" refers to more than two (including two), unless otherwise clearly and specifically defined.

[0037] References herein to "embodiments" mean that a particular feature, structure, or characteristic described in connection with the embodiments may be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it constitute an independent or alternative embodiment that is mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0038] In the description of the embodiments of this application, the term "and / or" is simply a description of the association relationship between associated objects, indicating that three relationships can exist. For example, A and / or B can represent the following three situations: A exists alone, A and B exist simultaneously, and B exists alone. In addition, the character " / " in this document generally indicates that the associated objects are in an "or" relationship.

[0039] Amounts, ratios, and other numerical values ​​are presented herein in a range format. It should be understood that such range format is used for convenience and brevity and should be interpreted flexibly to include not only the values ​​explicitly specified as range limits, but also all individual values ​​or sub-ranges encompassed within the range, as if each value and sub-range were explicitly specified.

[0040] If not otherwise specified, all steps of the present application may be performed sequentially, randomly, or in parallel, preferably sequentially. For example, the method includes steps (a) and (b), indicating that the method may include steps (a) and (b) performed sequentially, or may include steps (b) and (a) performed sequentially, or may include steps (a) and (b) performed simultaneously in parallel. For example, the method may further include step (c), indicating that step (c) may be added to the method in any order, for example, the method may include steps (a), (b), and (c), or may include steps (a), (c), and (b), or may include steps (c), (a), and (b), etc.

[0041] The skin is the body's first line of defense against external aggression. The skin barrier function maintains the normal operation of the skin's physiological functions by preventing the loss of water, nutrients, etc., while also ensuring that the organs and tissues in the body are protected from the invasion of harmful external substances, and plays an important role in maintaining the homeostasis of the body's internal environment.

[0042] The environmental challenges we face are becoming increasingly severe. On the one hand, strong sunlight, ultraviolet radiation from concentrated sunlight, air pollution from smog and dust, and the dryness and high humidity brought on by climate change pose significant challenges to skin health, leading to symptoms such as itching, redness, and discomfort, including dryness and tightness. On the other hand, the immense pressures of modern society and the demanding workload can easily lead to various negative emotions. Combined with unhealthy lifestyles, these factors can lead to sub-healthy or even damaged skin barriers, making them more susceptible to dehydration, oil-water imbalances, and allergic reactions such as redness, dryness, itching, stinging, and swelling.

[0043] Dry and sensitive skin is a vicious cycle that can worsen without intervention. When dry skin loses its natural protective barrier, it becomes more susceptible to external irritants and allergens. This makes the skin more susceptible to itching, redness, and inflammation, leading to increased sensitivity.

[0044] Based on this, the present application provides a skin care active ingredient and skin care product that can gently and efficiently produce moisturizing, soothing and repairing effects, effectively protect the skin barrier, maintain skin health, and has high safety.

[0045] The present invention provides a skin care active ingredient. The skin care active ingredient includes, by weight, 0.1-15 parts by weight of a moisturizer and 0.1-12 parts by weight of a soothing agent. The moisturizer includes at least one of quadruple hyaluronic acid and D-panthenol, and the quadruple hyaluronic acid includes sodium hyaluronate crosspolymer, sodium hyaluronate, acetylated sodium hyaluronate, or hydrolyzed sodium hyaluronate. The soothing agent includes at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol.

[0046] Quadruple hyaluronic acid acts as a skin hydrating factor, with multiple hyaluronic acids of varying molecular weights providing intense moisturization and repairing the skin barrier. D-panthenol significantly moisturizes and repairs the skin barrier in irritated skin, inhibiting the production of inflammatory factors and providing a soothing effect. Portulaca oleracea extract exhibits potent anti-inflammatory activity and soothes the skin. Kava kava extract also soothes and inhibits TRPV-1.

[0047] By designing skin care active ingredients including quadruple hyaluronic acid, at least one of D-panthenol, and at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol, multiple methods are combined to simultaneously solve the problems of skin inflammation and allergies and barrier reconstruction, and work together to achieve efficient and gentle moisturizing, repairing, and soothing effects, thereby improving skin condition and maintaining skin health.

[0048] In addition, the weight ratio of moisturizers and soothing agents in skin care actives is designed as above, so that the skin care actives can act on the skin to moisturize, repair, and soothe, and the effects are long-lasting without burdening the skin. It is safer, gently and efficiently improves skin condition, effectively protects the skin barrier, and maintains skin health.

[0049] It's important to note that healthy skin refers to skin that is smooth, soft, hydrated, even, firm, elastic, and flawless, based on the normal functioning of its physiological functions. Health is the foundation of beauty, and only healthy skin can achieve beauty. By empowering the skin with "balance, resilience, and self-healing abilities," we can gently and effectively maintain healthy skin.

[0050] Balance: The skin's surface and underlying layers, the stratum corneum and basal layer, each tightly bound together, enable the skin to maintain moisture and prevent water loss. The skin's ability to absorb and retain water, as well as the skin cells' ability to absorb and utilize oxygen, influence the skin's water balance and moisturizing capacity, as well as its metabolism and health, including its ability to heal itself.

[0051] Self-healing ability: The skin's ability to repair itself from damage, trauma, cell aging, etc., as well as the skin cells' ability to absorb and metabolize nutrients, affect the skin's metabolism and repair ability.

[0052] Resistance: The skin's ability to resist external environmental factors, including sunlight, pollutants, ultraviolet rays, bacteria, etc., as well as the skin cells' ability to identify and resist external pathogens and viruses, affect the health and appearance of the skin.

[0053] The quadruple hyaluronic acid in this application corresponds to "balancing power," D-panthenol corresponds to "self-healing power," purslane extract and dipotassium glycyrrhizate correspond to "self-healing power," and kava extract and trans-4-tert-butyl transhexanol correspond to "self-healing power." This application designs skincare active ingredients to include at least one of quadruple hyaluronic acid and D-panthenol, as well as at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol. This allows the skincare active ingredients to include both "balancing power" and "self-healing power," fundamentally improving the skin and gently and efficiently maintaining healthy skin.

[0054] From another perspective, based on the framework of "balance, resistance, and self-healing power", the use of a combination of active ingredients that are "homologous to the human body and originate from plants" can gently and efficiently achieve moisturizing, soothing, and repairing effects, and promote the skin to a healthy state. The 4-fold hyaluronic acid and D-panthenol in this application are ingredients that are homologous to the human body, and purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol are ingredients of plant origin. This application designs skin care active ingredients to include at least one of 4-fold hyaluronic acid and D-panthenol, and at least one of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol, so that the skin care active ingredients have both human homologous ingredients and plant origin ingredients, and achieve moisturizing, soothing, and repairing effects efficiently and gently.

[0055] In one embodiment, the skin care active comprises, by weight, 0.1-10 parts by weight of quadruple hyaluronic acid and / or 0.1-5 parts by weight of D-panthenol. It will be appreciated that when the moisturizing agent of the skin care active comprises quadruple hyaluronic acid, the skin care active comprises 0.1-10 parts by weight of quadruple hyaluronic acid; and when the moisturizing agent of the skin care active comprises D-panthenol, the skin care active comprises 0.1-5 parts by weight of D-panthenol. When the moisturizing agent of the skin care active comprises quadruple hyaluronic acid and D-panthenol, the skin care active comprises 0.1-10 parts by weight of quadruple hyaluronic acid and 0.1-5 parts by weight of D-panthenol. By designing the weight ratios of quadruple hyaluronic acid and / or D-panthenol as described above, the skin care active achieves moisturizing and repairing benefits without burdening the skin, resulting in a higher safety profile.

[0056] Optionally, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate in the quadruple hyaluronic acid is (0.2-0.5): (0.5-1): (0.5-1.2): (0.1-0.2). Exemplary skin care actives include: sodium hyaluronate cross-linked polymer with a molecular weight greater than 3000KDa (kilodaltons), sodium hyaluronate with a molecular weight of 1000-1800KDa, acetylated sodium hyaluronate with a molecular weight of 1000KDa, and hydrolyzed sodium hyaluronate with a molecular weight of less than 10KDa. By designing the molecular weights of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate as described above, the skin is covered from the surface to the deep layer of the skin, the skin barrier function is strengthened, moisture is locked in, and a good moisturizing effect is achieved. For example, the weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate can be 0.3:0.5:1:0.15, 0.2:0.7:0.8:0.1, 0.5:0.8:1.2:0.2, 0.4:1:1:0.1, etc. The components of the quadruple hyaluronic acid in this application are the same as above and will not be repeated here.

[0057] In one embodiment, the skin care active comprises, by weight, at least one of: 0.5-5 parts by weight of purslane extract, 0.5-5 parts by weight of dipotassium glycyrrhizate, 0.1-1 parts by weight of kava extract, and 0.1-1 parts by weight of trans-4-tert-butyl transhexanol. It will be appreciated that when the soothing agent of the skin care active comprises purslane extract, the skin care active comprises 0.5-5 parts by weight of purslane extract; and when the soothing agent of the skin care active comprises kava extract, the skin care active comprises 0.1-1 parts by weight of kava extract. By designing the weight ratios of purslane extract, dipotassium glycyrrhizate, kava extract, and trans-4-tert-butyl transhexanol as described above, the skin care active achieves a soothing effect while not burdening the skin and providing increased safety.

[0058] In one embodiment, the skin care active further comprises 0.1-15 parts by weight of an anti-allergic agent, and the anti-allergic agent comprises at least one of a kelp extract, a polygonatum extract, and a panax notoginseng extract. The anti-allergic agent has anti-inflammatory effects; for example, the kelp extract has the effects of improving microcirculation, anti-inflammatory, and antioxidant. By making the skin care active comprise an anti-allergic agent, the skin care active has anti-allergic effects; by designing the weight ratio of the anti-allergic agent as described above, the skin care active achieves anti-allergic effects without burdening the skin, and has higher safety. Optionally, when the anti-allergic agent is composed of a kelp extract, the skin care active comprises 0.1-5 parts by weight of a kelp extract. Optionally, the skin care active further comprises at least one of 0.1-5 parts by weight of a kelp extract, 0.1-5 parts by weight of a polygonatum extract, and 0.1-5 parts by weight of a panax notoginseng extract.

[0059] In addition, Kirinja Extract, Polygonatum Sibiricum Extract, and Panax Notoginseng Extract correspond to the "balancing force" and can further improve the skin's fundamentals, gently and effectively maintaining healthy skin. Kirinja Extract, Polygonatum Sibiricum Extract, and Panax Notoginseng Extract are plant-derived ingredients that gently and effectively achieve anti-allergic effects.

[0060] In one embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract.

[0061] In one embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, and 0.1-1 part of kava extract.

[0062] In one embodiment, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, and 0.1-5 parts by weight of unicorn extract.

[0063] In one embodiment, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts of D-panthenol, 0.1-1 parts of kava extract, and 0.1-5 parts of unicorn extract.

[0064] In one embodiment, the skin care active is composed of the following components in the following weight ratio: 0.1-10 parts by weight of quadruple hyaluronic acid, 0.5-5 parts by weight of purslane extract, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract.

[0065] In one embodiment, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, 0.1-1 parts of kava extract, and 0.1-5 parts by weight of unicorn extract.

[0066] In one embodiment, the skin care active ingredient is composed of the following components in the following weight ratio: 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of purslane extract, and 0.1-1 parts of kava extract.

[0067] It should be noted that the skin care active includes quadruple hyaluronic acid, and the weight parts of the quadruple hyaluronic acid in the skin care active can be 0.1 weight part, 0.5 weight part, 1 weight part, 1.5 weight parts, 2 weight parts, 2.5 weight parts, 3 weight parts, 3.5 weight parts, 4 weight parts, 4.5 weight parts, 5 weight parts, 5.5 weight parts, 6 weight parts, 6.5 weight parts, 7 weight parts, 7.5 weight parts, 8 weight parts, 8.5 weight parts, 9 weight parts, 10 weight parts, etc., or a range consisting of any two of the above values, for example, 0.1-5 weight parts, 3-8 weight parts, etc.

[0068] When the skin care active includes D-panthenol, the weight amount of D-panthenol in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, etc., or a range consisting of any two of the above values, such as 0.1-2 parts by weight, 1-4 parts by weight, etc.

[0069] When the skin care active includes at least one of purslane extract and dipotassium glycyrrhizate, the weight parts of purslane extract and dipotassium glycyrrhizate in the skin care active can be 0.5 weight parts, 1 weight part, 1.5 weight parts, 2 weight parts, 2.5 weight parts, 3 weight parts, 3.5 weight parts, 4 weight parts, 4.5 weight parts, 5 weight parts, etc., respectively, or a range consisting of any two of the above values, for example, 1-3.5 weight parts, 0.5-2 weight parts, etc.

[0070] When the skin care active includes at least one of kava extract and trans-4-tert-butyl transhexanol, the weight amounts of kava extract and trans-4-tert-butyl transhexanol in the skin care active can be 0.1 part by weight, 0.15 part by weight, 0.20 part by weight, 0.25 part by weight, 0.3 part by weight, 0.35 part by weight, 0.4 part by weight, 0.45 part by weight, 0.5 part by weight, 0.55 part by weight, 0.6 part by weight, 0.65 part by weight, 0.7 part by weight, 0.75 part by weight, 0.8 part by weight, 0.85 part by weight, 0.9 part by weight, 0.95 part by weight, 1 part by weight, etc., respectively, or a range consisting of any two of the above values, for example, 0.2-0.6 part by weight, 0.1-0.7 part by weight, etc.

[0071] When the skin care active includes at least one of the kelp extract, polygonatum extract, and panax notoginseng extract, the weight parts of the kelp extract, polygonatum extract, and panax notoginseng extract in the skin care active can be 0.1 parts by weight, 0.5 parts by weight, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, etc., respectively, or a range consisting of any two of the above values, for example, 0.5-2 parts by weight, 2-4 parts by weight, etc.

[0072] The embodiments of the present application also provide a skin care product, which includes the skin care active ingredients introduced in the above embodiments and has at least the same advantages as the above skin care active ingredients.

[0073] In one embodiment, the skin care product comprises, by weight, 0.2-42 parts of the skin care active and 5.6-13 parts of the base. Alternatively, the skin care product comprises, by weight, 0.4-26 parts of the skin care active. The base typically includes a moisturizing ingredient. It should be noted that the composition and proportions of the skin care active are as described above and will not be further elaborated.

[0074] The combination of skin care active ingredients and base materials forms a skin care product that can gently and effectively produce moisturizing, soothing and repairing effects, effectively protecting the skin barrier and maintaining skin health.

[0075] In one embodiment, the skin care product comprises a skin care active ingredient, a base, and deionized water. Optionally, the amount of deionized water to be added is calculated from the total weight of the skin care product being 100 parts by weight minus the sum of the weight of the skin care active ingredient and the weight of the base.

[0076] In one embodiment, the dosage form of the skin care product includes any one of a water-based solution, an emulsion, a cream, an essence lotion, a facial mask, and a spray. The skin care active ingredient can achieve antioxidant efficacy in any of these dosage forms, and the dosage form is selected based on specific needs; the base ingredients are designed based on the desired dosage form.

[0077] In one embodiment, the base comprises the following components in the following weight ratios: 2-5 parts by weight of glycerin, 1-2 parts by weight of polydimethylsiloxane, 2-4 parts by weight of squalane, 0.1-0.5 parts by weight of acrylic acid, acrylic acid esters, or C10-30 alkyl acrylate crosspolymer, and 0.5-1.5 parts by weight of polysorbate 60. This base is suitable for use in serum emulsion formulations, where polysorbate 60 provides an emulsifying effect and acrylic acid esters provide a thickening effect.

[0078] In one embodiment, the skin care product comprises the following components in the following weight ratios: 0.1-10 parts by weight of tetrahyaluronic acid, 0.1-5 parts of D-panthenol, 0.5-5 parts by weight of Portulaca oleracea extract, 0.1-1 parts of Piper methysticum extract, 0.1-5 parts by weight of Kirindra chinensis extract, 2-5 parts by weight of glycerin, 1-2 parts by weight of polydimethylsiloxane, 2-4 parts by weight of squalane, 0.1-0.5 parts by weight of acrylic acid, acrylates, or C10-30 alkyl acrylate crosspolymer, 0.5-1.5 parts by weight of polysorbate 60, and the remaining parts by weight of deionized water. The total weight of the skin care product is 100 parts. The weight ratio of sodium hyaluronate cross-linked polymer, sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed sodium hyaluronate in the quadruple hyaluronic acid is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

[0079] The skin care actives provided in this application have moisturizing, soothing, and repairing effects, and relevant experimental tests and studies have been conducted at the cellular level, biochemical reagent level, and skin model level. The following skin care product is composed of the following components in the following weight ratio: 5 parts by weight of 4-fold hyaluronic acid (the weight ratio of sodium hyaluronate crosspolymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate in the 4-fold hyaluronic acid is 0.4:1:1:0.1), 2.5 parts by weight of D-panthenol, 2 parts by weight of Portulaca oleracea extract, 1 part by weight of Kava Piper methysticum extract, 5 parts by weight of Kirin extract, 5 parts by weight of glycerin, 1.5 parts by weight of polydimethylsiloxane, 2 parts by weight of squalane, 0.5 parts by weight of C10-30 alkyl acrylate crosspolymer, 1 part by weight of polysorbate 60, and 74.5 parts by weight of deionized water as an example, and experimental tests at the cellular level, biochemical reagent level, and skin model level were conducted.

[0080] 1. Cell proliferation activity assay.

[0081] Experimental steps:

[0082] (1) HSF cells were seeded into 96-well plates, and 100 μL of culture medium containing 8*103 HSF cells was added to each well. The plates were incubated at 37°C for 24 h until the cells adhered to the plate and entered the logarithmic growth phase.

[0083] (2) After 24 hours, the culture medium in step (1) was discarded, and 100 μL of the test solution of different concentrations was added to each well. Three parallel wells were set for each concentration, and the cells were incubated for 24 hours.

[0084] (3) Aspirate the test solution added in step (2), add 90 μL of fresh culture medium and 10 μL of CCK-8 solution (Cell Counting Kit-8 reagent), and incubate for 1 to 4 hours.

[0085] (4) Measure the absorbance at 450 nm using an enzyme-labeled instrument.

[0086] Calculation method:

[0087] Cell viability* (%) = [A(sample added) - A(blank) / [A(0 sample added) - A(blank)] × 100;

[0088] Wherein, A(sample added) represents the absorbance of the wells containing cells, CCK-8 solution, and test solution;

[0089] A (blank) indicates the absorbance of wells with culture medium and CCK-8 solution but no cells;

[0090] A(0 loading) represents the absorbance of the wells containing cells and CCK-8 solution but no test solution;

[0091] Cell viability* indicates cell proliferation activity or cytotoxic activity.

[0092] It should be noted that when adding the culture medium containing CCK-8 to the cells in step (3), several blank wells without cells are added simultaneously to correct the values, which are referred to as A (blank). In step (2), several wells do not contain the test solution, but only the cell culture medium, which are referred to as A (0 addition).

[0093] Experimental results:

[0094] The experimental results are shown in Figure 1, which is a comparison chart of the cell proliferation activity test results. According to Figure 1, for the skin care active ingredients, cell proliferation activity tests were conducted on cells using concentrations of 5%, 1%, 0.5%, 0.1%, 0.05%, and 0.01%, respectively. The concentration here refers to the concentration of the skin care product diluted with cell culture medium, i.e., the "different concentrations of test solution" in step (2). Excellent proliferation activity was expressed at concentrations of 0.05%, 0.1%, 0.5%, and 1%, with increases of 4.3%, 7.4%, 9.7%, and 7.1%, respectively.

[0095] 2. Hyaluronidase activity test.

[0096] Experimental steps:

[0097] (1) Add 0.1 mL of CaCl solution (2.5 mol / L) to tubes A, B, C, and D, respectively.

[0098] (2) Add 0.5 mL of hyaluronidase solution (1250 U / mL) to tubes A and C.

[0099] (3) Replace the contents of tubes B and D with an equal amount of acetate buffer solution (pH = 5.6; a mixture of 0.2 mol / L sodium acetate and 0.2 mol / L acetic acid, 9.5:1) and keep them warm at 37°C for 20 min.

[0100] (4) Then add 0.5 mL of sample solution to tubes C and D, replace it with acetate buffer solution in tubes A and B, and keep it at 37°C for 20 min.

[0101] (5) Add 0.5 mL of sodium hyaluronate solution (0.5 mg / mL) to each tube, keep it at 37°C for 30 min, let it stand at room temperature for 5 min, and then place it in an 80°C water bath for 20 min.

[0102] (6) After taking out, add 0.1 mL of NaOH solution (0.4 mol / L) and 0.5 mL of acetylacetone solution to each tube, boil in water for 15 minutes, and then quickly ice-bathe for 5 minutes.

[0103] (7) Then add 1 mL of color developer and let it stand for 20 minutes for color development.

[0104] (8) The absorbance was measured at a wavelength of 547 nm, and the hyaluronidase inhibition rate was calculated according to the formula.

[0105] It should be noted that in the hyaluronidase activity experiment, the skin care product provided in the embodiment of the present application, quadruple hyaluronic acid, D-panthenol, purslane extract, kava extract, and kelp extract provided in the embodiment of the present application were respectively used as samples in step (2), and the experiment was carried out in sequence. The concentration of D-panthenol was the same as the concentration of D-panthenol in the skin care active of the skin care product; the concentration of purslane extract was the same as the concentration of purslane extract in the skin care active of the skin care product; the concentration of kava extract was the same as the concentration of kava extract in the skin care active of the skin care product; the concentration of quadruple hyaluronic acid was the same as the concentration of quadruple hyaluronic acid in the skin care active of the skin care product and had the same composition as the quadruple hyaluronic acid in the skin care active; the concentration of kelp extract was the same as the concentration of kelp extract in the skin care active of the skin care product.

[0106] Experimental results:

[0107] The experimental results are shown in Table 1. At a concentration of 10%, the skin care active ingredient has an inhibitory activity of 91.2% on hyaluronidase, and the activity of the skin care active ingredient is significantly higher than any monomer component. The results show that the skin care active ingredient provided in this application can significantly inhibit the activity of hyaluronidase and has a significant soothing effect. Among them, the concentration of the quadruple hyaluronic acid of the skin care active ingredient at a concentration of 10% is equivalent to 0.1%, the concentration of the D-panthenol of the skin care active ingredient at a concentration of 10% is equivalent to 0.1%, the concentration of the purslane extract of the skin care active ingredient at a concentration of 10% is equivalent to 0.5%, the concentration of the kava pepper extract of the skin care active ingredient at a concentration of 10% is equivalent to 0.1%, and the concentration of the unicorn extract of the skin care active ingredient at a concentration of 10% is equivalent to 0.1%.

[0108] Table 1 Hyaluronidase test results of skin care actives and active monomers of skin care actives

[0109] 3. Cell scratch test.

[0110] Experimental steps:

[0111] (1) HaCaT cells were seeded into 12-well plates and incubated at 37°C for 24 h. The cells adhered to the plate and entered the logarithmic growth phase.

[0112] (2) After 24 hours, discard the culture medium and add 100 μL of 0.05% concentration test solution to each well. Set up three parallel wells and incubate for 24 hours.

[0113] (3) Use a 100 μl pipette tip to make three parallel slits in each well, wash with PBS to remove the cells, add serum-free culture medium, take a photo under a microscope at 0 h, and continue to culture in the incubator for 24 h.

[0114] (4) After 24 hours, take photos under a microscope.

[0115] (5) Calculate the cell migration rate after 24 hours.

[0116] Experimental results:

[0117] The experimental results are shown in Figures 2 and 3, with Figure 2 being a cell scratch comparison chart and Figure 3 being a cell scratch test data comparison chart. As shown in Figures 2 and 3, the skin care products provided in the examples of the present application can significantly promote cell migration and have a significant repair effect; compared to the blank control group (pure culture medium, no test solution added) with a cell migration rate of 36.7%, the cell migration rate of the skin care product composition provided in the examples of the present application was 56.0%, and the increase rate was (56.0% - 36.7%) / 36.7% = 52.6%.

[0118] 4. Mitochondrial membrane potential detection experiment.

[0119] Experimental steps:

[0120] (1) Cell preparation: Cells were plated one day before the test to ensure that the cell confluence reached 50-70% at the time of the test.

[0121] (2) Sample addition: After 24 hours, the culture medium was discarded and 100 μL of 0.05% concentration of the test solution was added to each well. Three parallel wells were set up and incubated for 24 hours. The culture medium was prepared by mixing DMEM with fetal bovine serum in a ratio of 9:1.

[0122] (3) Aspirate the culture medium and wash the cells once with PBS or other appropriate solution if necessary according to the specific experiment.

[0123] (4) Drug induction: Remove the cell culture medium, dilute H2O2 to a concentration of 2 mmol / L with serum-free culture medium, and incubate in a cell culture incubator at 37°C in the dark for 4 hours.

[0124] (5) Aspirate the culture medium, wash the cells once with PBS, add 1 mL of JC-1 staining solution, and mix thoroughly. Incubate in a cell culture incubator at 37°C for 20 minutes. The JC-1 stock solution is 500X (that is, 500 times, the concentration of the staining solution is 1 times, that is, 1X, and the JC-1 staining solution is the 1X concentration after dilution).

[0125] (6) During the incubation period, prepare an appropriate amount of JC-1 staining buffer (1×) by adding 4 mL of distilled water to every 1 mL of JC-1 staining buffer (5×) and place it in an ice bath.

[0126] (7) After incubation at 37°C, remove the supernatant and wash twice with JC-1 staining buffer (1×).

[0127] (8) Add 2 mL of cell culture medium containing serum and phenol red.

[0128] (9) Observe under a fluorescence microscope or laser confocal microscope.

[0129] Experimental results:

[0130] The experimental results are shown in Figures 4 and 5. Figure 4 is a comparison chart of mitochondrial membrane potential, and Figure 5 is a comparison chart of mitochondrial membrane potential test data. As shown in Figures 4 and 5, the skin care products provided in the examples of this application can significantly reduce damage to mitochondria and have a significant mitochondrial protective effect. It should be noted that the blank control group did not add any samples and did not undergo H2O2 induction; the model group did not add any samples and underwent H2O2 induction; the composition refers to the group to which skin care active ingredients were added and H2O2 induction was performed.

[0131] 5. qPCR experiment.

[0132] Experimental steps:

[0133] (1) Cells were seeded into 24-well plates and incubated at 37°C for 24 h to allow the cells to adhere to the plate and enter the logarithmic growth phase.

[0134] (2) After 24 hours, discard the culture medium and add 100 μL of 0.05% concentration test solution to each well. Set up three parallel wells and incubate for 24 hours.

[0135] (3) Extract cellular RNA and reverse transcribe it into cDNA.

[0136] (4) Perform qPCR.

[0137] Experimental results:

[0138] The experimental results are shown in Figures 6 and 7. Figure 6 is a comparison chart of the IL-1α, IL-6, and PGE2 test results, and Figure 7 is a comparison chart of the TNF-α and TRPV1 test results. As shown in Figures 6 and 7, the skin care products provided in the examples of the present application can significantly reduce the expression levels of IL-1α, IL-6, PGE2, TNF-α, and TRPV1, and have significant anti-inflammatory and soothing effects. Compared with the model group, the levels were reduced by 23%, 31%, 89%, 42%, and 30%, respectively, showing a significant repair effect.

[0139] Through tests at the cellular level, biochemical reagent level, and skin model level, tests of hyaluronidase inhibition activity, cell migration rate, mitochondrial activity, IL-1α, IL-6, PGE2, TNF-α, and TRPV1 were carried out, respectively, verifying that the skin care active ingredients of this application can effectively promote moisturizing, soothing, and repairing effects.

[0140] Experimental studies were conducted on the components and amounts of the skin care actives provided herein. The differences between the examples are as follows: the components and amounts of the skin care actives, as shown in Table 5, are identical in the base material used in each example, specifically: 4 parts by weight of glycerin, 2 parts by weight of polydimethylsiloxane, 2 parts by weight of squalane, 0.4 parts by weight of acrylates, 1 part by weight of polysorbate 60, and the remaining parts by weight of deionized water, for a total of 100 parts by weight. In Examples 1-13, the sodium hyaluronate crosspolymer of tetrahyaluronic acid, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate were present in the same weight ratio of 0.4:1:1:0.1.

[0141] Table 2 Test parameters of various embodiments

[0142] As can be seen from Examples 1 to 15 above, the skin care actives provided in the present application have moisturizing, soothing, and repairing effects. It should be noted that the specific testing methods for hyaluronidase inhibition activity and cell scratch repair in each example in Table 2 can be found in the relevant descriptions of the above examples and will not be repeated here.

[0143] The specific test method of the stratum corneum water content of each embodiment in Table 2 is as follows:

[0144] (1) Test equipment

[0145] Stratum corneum moisture tester: an instrument that detects the moisture content of the skin's stratum corneum using the capacitance method.

[0146] (2) Environmental conditions

[0147] During the test, the test conditions at any test time point should remain consistent, such as the tester, location, instrument, etc.; instrument testing should be carried out in an environment with a temperature of (21±1)℃ and a relative humidity of (50±10)%. The subjects should adapt to this environmental condition for at least 30 minutes before evaluation and testing.

[0148] (3) Test process

[0149] 3.1 Screening

[0150] Recruit subjects as required and have them sign informed consent forms; ask subjects relevant questions based on the inclusion and exclusion criteria, and record them together with the test screening results.

[0151] Lactic acid sting test: Randomly select one side of the left or right cheek, take 50 μL of 10% lactic acid solution and apply it to the nasolabial groove of the cheek. The subjects will score the degree of stinging at 2.5 minutes and 5 minutes respectively according to the 4-point scale (0 point: no pain, 1 point: mild stinging, 2 points: moderate stinging, 3 points: severe stinging). The subjects with a total score of 3 points or more are judged to be lactic acid sting-positive.

[0152] 3.2 Cleaning

[0153] Before starting the test, the test area should be cleaned with warm water and dried with a lint-free paper towel; the cleaning process and water temperature should remain consistent at each follow-up visit.

[0154] 3.3 Testing

[0155] Instrument testing is performed on subjects at the initial visit and at various subsequent visits, and data are recorded. Multiple visits can be scheduled based on evaluation needs, typically over a 4-week period.

[0156] 3.4 Stratum corneum water content test

[0157] At each visit time point, the stratum corneum moisture content was measured using a stratum corneum moisture tester.

[0158] The above description is only an implementation method of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the description and drawings of this application, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A skin care active ingredient, wherein, the skin care active ingredient comprises 0.1 - 15 parts by weight of a humectant and 0.1 - 12 parts by weight of a soothing agent according to the weight ratio; wherein, the humectant comprises at least one of tetra - hyaluronic acid and D - panthenol, and the tetra - hyaluronic acid comprises sodium hyaluronate cross - polymer, sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed sodium hyaluronate; the soothing agent comprises at least one of purslane extract, dipotassium glycyrrhizinate, kava pepper extract, and trans - 4 - tert - butylcyclohexanol.

2. The skin care active substance according to claim 1, wherein The skin care active ingredient comprises 0.1 - 10 parts by weight of tetra - hyaluronic acid and / or 0.1 - 5 parts by weight of D - panthenol according to the weight ratio.

3. The skin care active substance according to claim 1, wherein The skin care active ingredient comprises at least one of 0.5 - 5 parts by weight of purslane extract, 0.5 - 5 parts by weight of dipotassium glycyrrhizinate, 0.1 - 1 part by weight of kava pepper extract, and 0.1 - 1 part by weight of trans - 4 - tert - butylcyclohexanol according to the weight ratio.

4. The skin care active substance according to claim 1, wherein, The skin care active ingredient further comprises 0.1 - 15 parts by weight of an anti - allergy agent, and the anti - allergy agent comprises at least one of dragon's blood extract, polygonatum extract, and notoginseng extract.

5. The skin care active substance according to claim 1, wherein, [[ID=]]The skin care active ingredient is composed of the following components in the following weight ratio: 0.1 - 10 parts by weight of tetra - hyaluronic acid, 0.1 - 5 parts of D - panthenol, 0.5 - 5 parts by weight of purslane extract, 0.1 - 1 part of kava pepper extract, 0.1 - 5 parts by weight of dragon's blood extract; or, The skin care active ingredient is composed of the following components in the following weight ratio: 0.1 - 10 parts by weight of tetra - hyaluronic acid, 0.1 - 5 parts of D - panthenol, 0.5 - 5 parts by weight of purslane extract, 0.1 - 1 part of kava pepper extract; or, The skin care active ingredient is composed of the following components in the following weight ratio: 0.1 - 10 parts by weight of tetra - hyaluronic acid, 0.1 - 5 parts of D - panthenol, 0.5 - 5 parts by weight of purslane extract, 0.1 - 5 parts by weight of dragon's blood extract; or, The skin care active ingredient is composed of the following components in the following weight ratio: 0.1 - 10 parts by weight of tetra - hyaluronic acid, 0.1 - 5 parts of D - panthenol, 0.1 - 1 part of kava pepper extract, 0.1 - 5 parts by weight of dragon's blood extract; or, The skin care active ingredient is composed of the following components in the following weight ratio: 0.1 - 10 parts by weight of tetra - hyaluronic acid, 0.5 - 5 parts by weight of purslane extract, 0.1 - 1 part of kava pepper extract, 0.1 - 5 parts by weight of dragon's blood extract; or, The skin care active ingredient is composed of the following components in the following weight ratio: 0.1 - 5 parts of D - panthenol, 0.5 - 5 parts by weight of purslane extract, 0.1 - 1 part of kava pepper extract, 0.1 - 5 parts by weight of dragon's blood extract; or, The skin care active ingredient is composed of the following components in the following weight ratio: 0.1 - 5 parts of D - panthenol, 0.5 - 5 parts by weight of purslane extract, 0.1 - 1 part of kava pepper extract.

6. The skin care active substance according to any one of claims 1-5, wherein, The weight ratio of the sodium hyaluronate cross-linked polymer, the sodium hyaluronate, the acetylated sodium hyaluronate, and the hydrolyzed sodium hyaluronate in the quadruple hyaluronic acid is (0.2-0.5):(0.5-1):(0.5-1.2):(0.1-0.2).

7. A skin care product, wherein, Comprising the skin care active ingredient according to any one of claims 1 to 6.

8. The skin care product according to claim 7, wherein, The skin care product comprises 0.2-42 parts by weight of the skin care active ingredient and 5.6-13 parts by weight of the base material according to the weight ratio.

9. The skin care product according to claim 8, wherein The skin care product is composed of the skin care active ingredient, the base material and deionized water.

10. The skin care product according to claim 8 or 9, wherein, The base material is composed of components in the following weight ratio: 2-5 parts by weight of glycerol, 1-2 parts by weight of polydimethylsiloxane, 2-4 parts by weight of squalane, 0.1-0.5 parts by weight of an acrylic acid or acrylate or C10-30 alkanol acrylate cross-linked polymer, 0.5-1.5 parts by weight of polysorbate-60.

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