A skin conditioner, its preparation and use

Abstract

The application provides a skin conditioner and a preparation method and application thereof, and belongs to the technical field of cosmetics.The skin conditioner is prepared from the following raw materials in parts by weight: 5-7 parts of spilanthes acmella extract, 0.5-1 part of a penetration enhancer composition, 0.2-0.4 part of a peptide, 1-2 parts of sodium hyaluronate, 0.7-1.2 parts of ascorbic acid, 10-12 parts of ethanol, and 5-7 parts of water.The skin conditioner prepared by the application contains safe, mild and highly active spilanthes acmella extract, can obviously improve the absorption and utilization efficiency of components by the skin, has good activities of whitening, anti-aging, anti-inflammation, anti-allergy, anti-oxidation, moisturizing and anti-wrinkling, and has a wide application prospect.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a skin conditioning agent, its preparation method, and its application. Background Technology

[0002] Skin aging is a prominent external manifestation of human aging. As people age, their metabolism slows down, the number of fibroblasts in the body gradually decreases, and the collagen and elastin they secrete are gradually reduced and broken down due to oxidation. The supporting components of the epidermis disappear, causing uneven collapse of the epidermis, thinning of the dermis, loss of skin elasticity, and gradual dryness, sagging, dullness, and the formation of wrinkles.

[0003] CN110090166A discloses an anti-wrinkle composition comprising, by weight, the following components: 0.1-5 parts of acetyl hexapeptide-8, 0.1-5 parts of fibronectin, and 0.5-4.5 parts of cannabidiol. The components work synergistically to effectively reduce existing facial wrinkles, deeply nourish the skin, accelerate cell metabolism, enhance skin resistance, and prevent the formation of new wrinkles. Furthermore, the anti-wrinkle composition has good moisturizing properties, promotes the secretion of collagen and elastin by fibroblasts, strengthens the epidermis's support, protects the dermis, and enhances skin elasticity.

[0004] However, existing skincare products have the following drawbacks: 1. They use single-function moisturizers, resulting in unsatisfactory actual moisturizing effects on the skin; 2. They can only prevent wrinkles, without actually improving existing wrinkles, and their anti-wrinkle effects are not obvious; 3. They lack sufficient moisturizing power. Neck care products are often used with massage techniques, but if the product is not moisturizing enough, it can easily pull and damage the skin; 4. They have low penetration and poor absorption, with nutrients remaining on the skin's surface and difficult to be truly absorbed, resulting in no improvement in skin problems; 5. They often use methylisothiazolinone (MIT) and parabens as preservatives, which have a high sensitization rate.

[0005] purslane( Portulaca oleracea L.Purslane (Purslane var. purslane), also known as longevity vegetable or five-element herb, is widely distributed in tropical and subtropical regions worldwide. It is listed in the World Health Organization's list of commonly used medicinal plants and has been dubbed a "global panacea." It is also one of the medicinal and edible plants designated by the Chinese Ministry of Health. The Chinese Pharmacopoeia includes the aerial parts of purslane for medicinal use, possessing heat-clearing, detoxifying, blood-cooling, and hemostatic effects. It can be used for dysentery caused by heat toxins, carbuncles, boils, eczema, erysipelas, etc., and is rich in alkaloids, organic acids, terpenes, flavonoids, and other components. However, it also contains hormones such as norepinephrine and dopamine (Liu Di et al., Study on the Determination of Norepinephrine and Dopamine in Purslane). These components are prohibited in the 2015 edition of the "Cosmetic Safety Technical Specifications," and hormones can further damage the skin and increase sensitivity. Safe, effective, and reliable purslane extract products need further development and improvement. Summary of the Invention

[0006] The purpose of this invention is to propose a skin conditioning agent, its preparation method and application, which contains a safe, mild and highly active purslane extract, which can significantly improve the skin's absorption and utilization efficiency of components, and has excellent whitening, anti-aging, anti-inflammatory, anti-allergic, antioxidant, moisturizing and anti-wrinkle activities, and has broad application prospects.

[0007] The technical solution of this invention is implemented as follows: This invention provides a skin conditioning agent, prepared from the following raw materials in parts by weight: 5-7 parts of purslane extract, 0.5-1 parts of a penetration enhancer composition, 0.2-0.4 parts of peptides, 1-2 parts of sodium hyaluronate, 0.7-1.2 parts of ascorbic acid, 10-12 parts of ethanol, and 5-7 parts of water; The penetration enhancer composition comprises menthol and a penetration enhancer compound in a mass ratio of 1-2:5, and the structural formula of the penetration enhancer compound is shown in Formula I: Formula I.

[0008] As a further improvement of the present invention, the preparation method of the purslane extract is as follows: S1. Enzymatic fermentation: Wash, dry, and crush purslane, add it to water, heat to boiling to extract, then cool to room temperature, add compound enzyme, enzymatic hydrolysis, sterilize, inoculate with selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum seed liquid, enzymatic fermentation culture, filter, and collect filtrate. S2. Extraction of selenized polysaccharides: Ethanol is added to the filtrate from step S1 to precipitate the polysaccharides, which are then filtered and the solid is collected. The filtrate is retained, and the solid is washed and dried to obtain selenized polysaccharides. S3. Extraction of selenized protein: Dialyze the filtrate from step S2, collect the dialysate and permeate, freeze-dry the dialysate to obtain selenized protein; S4. Preparation of selenized protein titanium / copper complex: The selenized protein obtained in step S3 was dissolved in water, titanium salt and copper salt were added, the mixture was stirred and reacted, dialyzed, the dialysate was collected, and freeze-dried to obtain the selenized protein titanium / copper complex. S5. Preparation of active extract: Dichloromethane is added to the permeate, extraction is performed, solvent is recovered under reduced pressure, washing is performed, and drying is carried out to obtain the active extract; S6. Preparation of active extract liposomes: The active extract obtained in step S5, soybean lecithin, propylene glycol and polyethylene glycol are mixed and dissolved, water is added, ultrasonic treatment is performed, filtration is carried out, and freeze drying is performed to obtain active extract liposomes. S7. Preparation of purslane extract: The selenized polysaccharide obtained in step S2, the selenized protein titanium / copper complex obtained in step S4, and the active extract liposome obtained in step S6 are mixed evenly to obtain purslane extract.

[0009] As a further improvement of the present invention, the solid-liquid ratio of purslane and water in step S1 is 1:5-7 g / mL, the heating and boiling extraction time is 2-4 h, the compound enzyme includes cellulase, hemicellulase, and pectinase in a mass ratio of 4-7:1-2:2-4, the amount of the compound enzyme added is 2-4 wt% of the total mass of the system, the enzymatic hydrolysis temperature is 45-50℃, the time is 1-3 h, and the bacterial count of the selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum, and Bifidobacterium longum seed solution is 10. 8 -10 9 The inoculation amounts of the selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum, and Bifidobacterium longum seed culture were 2-4 v / v%, 0.5-1 v / v%, 0.2-0.5 v / v%, and 0.3-0.5 v / v, respectively. The enzymatic fermentation conditions were 40-45℃, 100-200 r / min, and 36-48 h. In step S2, ethanol was added until the ethanol content in the system was 80-85 wt%, and the precipitation time was 3-5 h.

[0010] As a further improvement of the present invention, the dialysis bag used for dialysis in step S3 has a pore size of 3k-5kDa, and the dialysis time is 5-7h; the mass ratio of selenized protein, titanium salt and copper salt in step S4 is 10-15:0.5-1:0.3-0.7, the titanium salt is selected from at least one of titanium chloride, titanium sulfate and titanium nitrate, the copper salt is selected from at least one of copper chloride, copper sulfate and copper nitrate, the stirring reaction time is 20-30min, the dialysis bag used for dialysis has a pore size of 3k-5kDa, and the dialysis time is 3-5h.

[0011] As a further improvement of the present invention, the volume ratio of the permeate and dichloromethane in step S5 is 1:0.7-1.2; the mass ratio of the active extract, soybean lecithin, propylene glycol, polyethylene glycol, and water in step S6 is 0.7-1.2:7-12:2-4:6-12:100; the ultrasonic treatment power is 500-700W and the time is 10-15min; the filtration uses a 0.22μm microporous membrane; and the mass ratio of the selenized polysaccharide, selenized protein titanium / copper complex, and active extract liposomes in step S7 is 3-5:2-4:0.7-1.2.

[0012] As a further improvement of the present invention, the preparation method of the purslane extract is as follows: S1. Enzymatic fermentation: Wash, dry, and crush purslane, add it to water, the solid-liquid ratio of purslane to water is 1:5-7 g / mL, heat to boiling and extract for 2-4 h, then cool to room temperature, add compound enzyme, the amount added is 2-4 wt% of the total mass of the system, enzymatic hydrolysis at 45-50℃ for 1-3 h, sterilize, inoculate with selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum seed liquid, the inoculation amount is 2-4 v / v%, 0.5-1 v / v%, 0.2-0.5 v / v% and 0.3-0.5 v / v%, respectively, at 40-45℃, 100-200 r / min, enzymatic fermentation for 36-48 h, filter, and collect the filtrate; The complex enzyme includes cellulase, hemicellulase, and pectinase in a mass ratio of 4-7:1-2:2-4. The bacterial counts of the selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum, and Bifidobacterium longum seed solutions were 10. 8 -10 9 cfu / mL; S2. Extraction of selenized polysaccharides: Add ethanol to the filtrate in step S1 until the ethanol content in the system is 80-85 wt%, precipitate for 3-5 hours, filter, collect the solid, keep the filtrate, wash the solid, dry it, and obtain selenized polysaccharides. S3. Extraction of selenized protein: Dialyze the filtrate from step S2 using a dialysis bag with a pore size of 3k-5kDa for 5-7 hours, collect the dialysate and permeate, freeze-dry the dialysate to obtain selenized protein; S4. Preparation of selenized protein titanium / copper complex: Dissolve 10-15 parts by weight of the selenized protein obtained in step S3 in 100 parts by weight of water, add 0.5-1 parts by weight of titanium salt and 0.3-0.7 parts by weight of copper salt, stir and react for 20-30 min, dialyze with a dialysis bag with a pore size of 3k-5kDa for 3-5 h, collect the dialysate, freeze dry, and obtain selenized protein titanium / copper complex; S5. Preparation of active extract: Dichloromethane was added to the permeate at a volume ratio of 1:0.7-1.2, and the extract was extracted. The solvent was recovered under reduced pressure, washed, and dried to obtain the active extract. S6. Preparation of active extract liposomes: Mix and dissolve 0.7-1.2 parts by weight of the active extract obtained in step S5, 7-12 parts by weight of soybean lecithin, 2-4 parts by weight of propylene glycol, and 6-12 parts by weight of polyethylene glycol, add 100 parts by weight of water, sonicate at 500-700W for 10-15 min, filter with a 0.22μm microporous membrane, and freeze-dry to obtain active extract liposomes; S7. Preparation of purslane extract: Mix 3-5 parts by weight of the selenized polysaccharide obtained in step S2, 2-4 parts by weight of the selenized protein titanium / copper complex obtained in step S4, and 0.7-1.2 parts by weight of the active extract liposome obtained in step S6 evenly to obtain purslane extract.

[0013] As a further improvement of the present invention, the method for preparing the permeation-enhancing compound is as follows: T1. will d -4-terpineol reacts with salicylic acid to produce salicylic acid. d -4-terpineol ester, with the following structure: ; T2. The dodecyl ester of 2-chloropropionic acid was prepared by reacting n-dodecyl alcohol with 2-chloropropionic acid, with the following structure: ; T3. Dodecyl 2-chloropropionate and salicylic acid d The product was obtained by reacting -4-terpineol alcohol ester.

[0014] As a further improvement of the present invention, the steps described in T1 are as follows: d The molar ratio of -4-terpineol to salicylic acid is 1:1.2-1.4, and concentrated sulfuric acid is added as a catalyst. The reaction temperature is 110-120℃, and the time is 5-7 h. In step T2, the molar ratio of n-dodecyl alcohol to 2-chloropropionic acid is 1:1.2-1.4, and ferric chloride is added as a catalyst. The reaction temperature is 100-120℃, and the time is 3-5 h. In step T3, dodecyl 2-chloropropionic acid ester and salicylic acid... d The molar ratio of 4-terpineol ester is 1.1-1.3:1, and a base is added, wherein the base is selected from at least one of triethylamine, NaOH, and KOH, and the reaction temperature is 45-50℃ for 4-6 hours.

[0015] The present invention further protects a method for preparing the above-mentioned skin conditioning agent, comprising the following steps: mixing purslane extract, penetration enhancer composition, peptide, sodium hyaluronate, ascorbic acid, ethanol and water evenly to obtain the skin conditioning agent.

[0016] This invention further protects the application of the above-mentioned skin conditioning agent in the preparation of whitening, anti-aging, anti-inflammatory, anti-allergic, antioxidant, moisturizing, and anti-wrinkle cosmetics.

[0017] The present invention has the following beneficial effects: This invention prepares a purslane extract through water extraction, enzymatic hydrolysis, and fermentation. Water extraction promotes the dissolution of active ingredients in purslane into the aqueous solution. During enzymatic hydrolysis, the plant cell walls undergo hydrolysis and rupture, allowing a large amount of polysaccharides, proteins, alkaloids, flavonoids, polyphenols, and organic acids to flow out of the cells. Further, under the fermentation action of fermenting bacteria, on the one hand, cell wall rupture is promoted, and the active ingredients are dissolved; on the other hand, the fermenting bacteria convert irritating hormones in purslane, including norepinephrine and dopamine, into safe small molecules, while also producing other fermentation active products such as short-chain fatty acids, further improving the safety and activity of the product. It has excellent whitening, anti-aging, anti-inflammatory, anti-allergic, antioxidant, and moisturizing activities.

[0018] The addition of selenium-enriched yeast results in a product rich in selenized proteins and selenized polysaccharides. The selenized proteins obtained through alcohol precipitation not only have excellent moisturizing and antioxidant effects, but also neutralize free radicals, reduce oxidative stress damage to the skin, thereby slowing down the aging process, maintaining skin surface moisture, improving dry skin, and making the skin softer and smoother. At the same time, they have certain anti-inflammatory and soothing effects, which can alleviate discomfort and redness caused by skin irritation or inflammation, and help promote collagen synthesis, improve skin elasticity, and reduce the appearance of fine lines and wrinkles.

[0019] Selenized protein is also an excellent supplement for skin collagen, capable of scavenging free radicals, reducing oxidative stress damage to the skin, regulating melanocyte activity, inhibiting melanin production, and reducing age spots and pigmentation. Rich in amino acids and proteins, it helps maintain skin moisture, improves skin hydration, reduces inflammation after skin irritation, and alleviates redness and itching. It also helps promote skin cell regeneration and repair, accelerating wound healing. The copper and titanium ions in the selenized protein titanium / copper complex, formed through the chelation of amino and carboxyl groups on amino acids, enhance the antibacterial and anti-inflammatory effects of the extract. Copper ions participate in collagen synthesis, helping to improve skin elasticity, reduce fine lines and wrinkles, promote skin cell repair and wound healing, and improve overall skin health. Titanium ions have strong adsorption capacity, absorbing oil on the skin surface, cleaning pores, and reducing problems caused by sebum secretion. They also have good photosensitivity, absorbing, refracting, or scattering ultraviolet rays, providing some sun protection. Furthermore, they have a soothing effect on sensitive skin, reducing itching, redness, and swelling. The two work together to achieve a very good skin conditioning effect.

[0020] The active extract obtained by adding dichloromethane to the permeate contains abundant polyphenols, flavonoids (including quercetin, genistein, luteolin, apigenin, myricetin, hesperidin, etc.), organic acids (linolenic acid, palmitic acid, etc.), and other active substances. It has excellent antioxidant, anti-inflammatory, soothing, and anti-allergic effects. Under the encapsulation of liposomes, on the one hand, the oxidative inactivation of the active extract can be reduced. On the other hand, since the surface of the liposome is a phospholipid layer, which is similar in structure to the phospholipid layer of the skin, they can be integrated with each other, thereby promoting the transdermal absorption of the active extract. This greatly enhances the activity of the active extract in the skin layer and improves the skin care effect.

[0021] The skin conditioning agent of this invention also contains a penetration enhancer composition, including menthol and penetration-enhancing compounds. Salicylic acid has excellent anti-inflammatory effects, but when used alone, it is highly irritating to the skin. d -4-Tepineol has excellent permeation-enhancing effects, but it has an unpleasant odor and high volatility. Therefore, the permeation-enhancing compound of this invention combines it with... d The product obtained from the reaction of -4-terpineol effectively masks the shortcomings of both. Further reaction with dodecanol 2-chloropropionate produces a permeation-enhancing compound whose long-chain alkyl chain can effectively disturb the skin's phospholipid layer, thereby significantly improving its permeation-enhancing effect. When used in combination with menthol, it has a synergistic effect.

[0022] The skin conditioning agent prepared by this invention contains safe, mild, and highly active purslane extract, which can significantly improve the skin's absorption and utilization efficiency of components. It has excellent whitening, anti-aging, anti-inflammatory, anti-allergic, antioxidant, moisturizing, and anti-wrinkle activities, and has broad application prospects. Detailed Implementation

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Preparation method of seed culture of selenium-enriched yeast (purchased from Angel Yeast Co., Ltd.) and Bacillus subtilis (purchased from Shaanxi Chenming Biotechnology Co., Ltd.): The inoculum was inoculated into Gao's medium and activated under aerobic conditions at 45℃ and 120 r / min for 24 h to obtain a culture with a bacterial count of 10. 8 -10 9 CFU / mL bacterial seed solution.

[0025] Preparation method of Lactobacillus plantarum (purchased from Shaanxi Chenming Biotechnology Co., Ltd.) and Bifidobacterium longum (purchased from Shaanxi Chenming Biotechnology Co., Ltd.) seed culture: The inoculum was inoculated into Gao's medium and activated under anaerobic conditions at 37℃ and 120 r / min for 24 h to obtain a bacterial count of 10. 8 -10 9 CFU / mL bacterial seed culture Cellulase, 50,000 U / g, hemicellulase, 50,000 U / g, pectinase, 25,000 U / g, purchased from Xiasheng (Beijing) Biotechnology Development Co., Ltd.

[0026] Preparation Example 1: Preparation of Portulaca oleracea extract The specific method is as follows: S1. Enzymatic fermentation: Wash, dry, and crush purslane, add it to water, the solid-liquid ratio of purslane to water is 1:5 g / mL, heat to boiling and extract for 2 hours, then cool to room temperature, add compound enzyme, the amount added is 2wt% of the total mass of the system, enzymatic hydrolysis at 45℃ for 1 hour, sterilize, inoculate with selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum seed liquid, the inoculation amount is 2v / v%, 0.5v / v%, 0.2v / v% and 0.3v / v%, respectively, 40℃, 100r / min, enzymatic fermentation for 36 hours, filter, and collect the filtrate; The complex enzyme comprises cellulase, hemicellulase, and pectinase in a mass ratio of 4:1:2. S2. Extraction of selenized polysaccharides: Add ethanol to the filtrate in step S1 until the ethanol content in the system is 80wt%, precipitate for 3h, filter, collect the solid, keep the filtrate, wash the solid, dry it, and obtain selenized polysaccharides. S3. Extraction of selenized protein: Dialyze the filtrate from step S2 through a dialysis bag with a pore size of 3 kDa for 5 hours, collect the dialysate and permeate, freeze-dry the dialysate to obtain selenized protein; S4. Preparation of selenized protein titanium / copper complex: 10 parts by weight of selenized protein obtained in step S3 were dissolved in 100 parts by weight of water, 0.5 parts by weight of titanium chloride and 0.3 parts by weight of copper chloride were added, the mixture was stirred and reacted for 20 min, dialyzed with a dialysis bag with a pore size of 3 kDa for 3 h, the dialysate was collected, and freeze-dried to obtain selenized protein titanium / copper complex. S5. Preparation of active extract: Dichloromethane was added to the permeate at a volume ratio of 1:0.7, and the extract was extracted. The solvent was recovered under reduced pressure, washed, and dried to obtain the active extract. S6. Preparation of active extract liposomes: 0.7 parts by weight of the active extract obtained in step S5, 7 parts by weight of soybean lecithin, 2 parts by weight of propylene glycol and 6 parts by weight of PEG400 were mixed and dissolved, 100 parts by weight of water were added, and the mixture was sonicated at 500W for 10 min. The mixture was then filtered through a 0.22 μm microporous membrane and freeze-dried to obtain active extract liposomes. S7. Preparation of purslane extract: 3 parts by weight of selenized polysaccharide obtained in step S2, 2 parts by weight of selenized protein titanium / copper complex obtained in step S4, and 0.7 parts by weight of active extract liposome obtained in step S6 were stirred and mixed for 10 min to obtain purslane extract.

[0027] Preparation Example 2: Preparation of Portulaca oleracea extract The specific method is as follows: S1. Enzymatic fermentation: Wash, dry, and crush purslane, add it to water, the solid-liquid ratio of purslane to water is 1:7 g / mL, heat to boiling and extract for 4 hours, then cool to room temperature, add compound enzyme, the amount added is 4wt% of the total mass of the system, enzymatic hydrolysis at 50℃ for 3 hours, sterilize, inoculate with selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum seed liquid, the inoculation amount is 4v / v%, 1v / v%, 0.5v / v% and 0.5v / v%, respectively, 45℃, 200r / min, enzymatic fermentation for 48 hours, filter, and collect the filtrate; The complex enzyme comprises cellulase, hemicellulase, and pectinase in a mass ratio of 7:2:4. S2. Extraction of selenized polysaccharides: Add ethanol to the filtrate in step S1 until the ethanol content in the system is 85wt%, precipitate for 5h, filter, collect the solid, keep the filtrate, wash the solid, dry it, and obtain selenized polysaccharides. S3. Extraction of selenized protein: Dialyze the filtrate from step S2 through a dialysis bag with a pore size of 5 kDa for 7 hours, collect the dialysate and permeate, freeze-dry the dialysate to obtain selenized protein; S4. Preparation of selenized protein titanium / copper complex: 15 parts by weight of selenized protein obtained in step S3 were dissolved in 100 parts by weight of water, 1 part by weight of titanium sulfate and 0.7 parts by weight of copper sulfate were added, the mixture was stirred and reacted for 30 min, dialyzed with a dialysis bag with a pore size of 5 kDa for 5 h, the dialysate was collected and freeze-dried to obtain selenized protein titanium / copper complex. S5. Preparation of active extract: Dichloromethane was added to the permeate at a volume ratio of 1:1.2, and the mixture was extracted. The solvent was recovered under reduced pressure, washed, and dried to obtain the active extract. S6. Preparation of active extract liposomes: 1.2 parts by weight of the active extract obtained in step S5, 12 parts by weight of soybean lecithin, 4 parts by weight of propylene glycol, and 12 parts by weight of PEG400 were mixed and dissolved, 100 parts by weight of water were added, and the mixture was sonicated at 700W for 15 min. The mixture was then filtered through a 0.22 μm microporous membrane and freeze-dried to obtain active extract liposomes. S7. Preparation of purslane extract: 5 parts by weight of selenized polysaccharide obtained in step S2, 4 parts by weight of selenized protein titanium / copper complex obtained in step S4, and 1.2 parts by weight of active extract liposome obtained in step S6 were stirred and mixed for 10 min to obtain purslane extract.

[0028] Preparation Example 3: Preparation of Portulaca oleracea extract The specific method is as follows: S1. Enzymatic fermentation: Wash, dry, and crush purslane, add it to water, the solid-liquid ratio of purslane to water is 1:6 g / mL, heat to boiling and extract for 3 hours, then cool to room temperature, add compound enzyme, the amount added is 3wt% of the total mass of the system, enzymatic hydrolysis at 47℃ for 2 hours, sterilize, inoculate with selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum seed liquid, the inoculation amount is 3v / v%, 0.7v / v%, 0.35v / v% and 0.4v / v%, respectively, enzymatic fermentation at 42℃ and 150r / min for 42 hours, filter, and collect the filtrate; The complex enzyme comprises cellulase, hemicellulase, and pectinase in a mass ratio of 5.5:1.2:3. S2. Extraction of selenized polysaccharides: Add ethanol to the filtrate in step S1 until the ethanol content in the system is 82wt%, precipitate for 4 hours, filter, collect the solid, keep the filtrate, wash the solid, dry it, and obtain selenized polysaccharides. S3. Extraction of selenized protein: Dialyze the filtrate from step S2 using a dialysis bag with a pore size of 4 kDa for 6 hours, collect the dialysate and permeate, freeze-dry the dialysate to obtain selenized protein; S4. Preparation of selenized protein titanium / copper complex: 12 parts by weight of selenized protein obtained in step S3 were dissolved in 100 parts by weight of water, 0.7 parts by weight of titanium nitrate and 0.5 parts by weight of copper nitrate were added, the mixture was stirred and reacted for 25 min, dialyzed with a dialysis bag with a pore size of 4 kDa for 4 h, the dialysate was collected, and freeze-dried to obtain selenized protein titanium / copper complex. S5. Preparation of active extract: Dichloromethane was added to the permeate at a volume ratio of 1:1, and the extract was extracted. The solvent was recovered under reduced pressure, washed, and dried to obtain the active extract. S6. Preparation of active extract liposomes: 1 part by weight of the active extract obtained in step S5, 10 parts by weight of soybean lecithin, 3 parts by weight of propylene glycol and 8 parts by weight of PEG400 were mixed and dissolved, 100 parts by weight of water were added, and the mixture was sonicated at 600W for 12 min. The mixture was then filtered through a 0.22 μm microporous membrane and freeze-dried to obtain active extract liposomes. S7. Preparation of purslane extract: 4 parts by weight of selenized polysaccharide obtained in step S2, 3 parts by weight of selenized protein titanium / copper complex obtained in step S4, and 1 part by weight of active extract liposome obtained in step S6 were stirred and mixed for 10 min to obtain purslane extract.

[0029] Preparation Example 4 The difference compared to Preparation Example 3 is that the complex enzyme is a single cellulase.

[0030] Preparation Example 5 The difference compared to Preparation Example 3 is that the complex enzyme is a single hemicellulase.

[0031] Preparation Example 6 The difference compared to Preparation Example 3 is that the complex enzyme is a single pectinase.

[0032] Comparative Preparation Example 1 The difference from preparation example 3 is that no complex enzyme was added in step S1.

[0033] Specifically as follows: S1. Fermentation: Wash, dry, and crush purslane, add it to water, and the solid-liquid ratio of purslane to water is 1:6 g / mL. Heat to boiling and extract for 3 hours, then cool to room temperature and sterilize. Inoculate with seed liquids of selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum, and Bifidobacterium longum, with inoculation amounts of 3 v / v%, 0.7 v / v%, 0.35 v / v%, and 0.4 v / v%, respectively. Ferment at 42℃ and 150 r / min for 42 hours. Filter and collect the filtrate.

[0034] Comparative Preparation Example 2 Compared with Preparation Example 3, the difference is that in step S1, only a single selenium-enriched yeast seed solution was inoculated, with an inoculation amount of 4.5 v / v.

[0035] Comparative preparation example 3 Compared with Preparation Example 3, the difference is that in step S1, no selenium-enriched yeast seed solution was inoculated, and the inoculation amounts of Bacillus subtilis, Lactobacillus plantarum, and Bifidobacterium longum seed solutions were 2.15 v / v%, 1.07 v / v%, and 1.23 v / v, respectively.

[0036] Comparative preparation example 4 The difference from Preparation Example 3 is that fermentation was not performed in step S1.

[0037] Specifically as follows: S1. Enzymatic hydrolysis: Wash, dry, and crush the purslane, add it to water, and the solid-liquid ratio of purslane to water is 1:6 g / mL. Heat to boiling and extract for 3 hours, then cool to room temperature, add compound enzyme at 3 wt% of the total mass of the system, hydrolyze at 47℃ for 2 hours, filter, and collect the filtrate.

[0038] Comparative preparation example 5 The difference from Preparation Example 3 is that titanium nitrate was not added in step S4, and the amount of copper nitrate added was 1.2 parts by weight.

[0039] Comparative preparation example 6 The difference from Preparation Example 3 is that copper nitrate was not added in step S4, and titanium nitrate was added in an amount of 1.2 parts by weight.

[0040] Comparative preparation example 7 The difference compared to preparation example 3 is that step S4 was not performed.

[0041] Specifically as follows: S1. Enzymatic fermentation: Wash, dry, and crush purslane, add it to water, the solid-liquid ratio of purslane to water is 1:6 g / mL, heat to boiling and extract for 3 hours, then cool to room temperature, add compound enzyme, the amount added is 3wt% of the total mass of the system, enzymatic hydrolysis at 47℃ for 2 hours, sterilize, inoculate with selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum seed liquid, the inoculation amount is 3v / v%, 0.7v / v%, 0.35v / v% and 0.4v / v%, respectively, enzymatic fermentation at 42℃ and 150r / min for 42 hours, filter, and collect the filtrate; The complex enzyme comprises cellulase, hemicellulase, and pectinase in a mass ratio of 5.5:1.2:3. S2. Extraction of selenized polysaccharides: Add ethanol to the filtrate in step S1 until the ethanol content in the system is 82wt%, precipitate for 4 hours, filter, collect the solid, keep the filtrate, wash the solid, dry it, and obtain selenized polysaccharides. S3. Extraction of selenized protein: Dialyze the filtrate from step S2 using a dialysis bag with a pore size of 4 kDa for 6 hours, collect the dialysate and permeate, freeze-dry the dialysate to obtain selenized protein; S4. Preparation of active extract: Dichloromethane was added to the permeate at a volume ratio of 1:1, and the extract was extracted. The solvent was recovered under reduced pressure, washed, and dried to obtain the active extract. S5. Preparation of active extract liposomes: 1 part by weight of the active extract obtained in step S4, 10 parts by weight of soybean lecithin, 3 parts by weight of propylene glycol and 8 parts by weight of PEG400 were mixed and dissolved, 100 parts by weight of water were added, and the mixture was sonicated at 600W for 12 min. The mixture was then filtered through a 0.22 μm microporous membrane and freeze-dried to obtain active extract liposomes. S6. Preparation of purslane extract: 4 parts by weight of selenized polysaccharide obtained in step S2, 3 parts by weight of selenized protein obtained in step S3, and 1 part by weight of active extract liposome obtained in step S5 are stirred and mixed for 10 min to obtain purslane extract.

[0042] Comparative Preparation Example 8 The difference from preparation example 3 is that step S6 was not performed.

[0043] Specifically as follows: S1. Enzymatic fermentation: Wash, dry, and crush purslane, add it to water, the solid-liquid ratio of purslane to water is 1:6 g / mL, heat to boiling and extract for 3 hours, then cool to room temperature, add compound enzyme, the amount added is 3wt% of the total mass of the system, enzymatic hydrolysis at 47℃ for 2 hours, sterilize, inoculate with selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum seed liquid, the inoculation amount is 3v / v%, 0.7v / v%, 0.35v / v% and 0.4v / v%, respectively, enzymatic fermentation at 42℃ and 150r / min for 42 hours, filter, and collect the filtrate; The complex enzyme comprises cellulase, hemicellulase, and pectinase in a mass ratio of 5.5:1.2:3. S2. Extraction of selenized polysaccharides: Add ethanol to the filtrate in step S1 until the ethanol content in the system is 82wt%, precipitate for 4 hours, filter, collect the solid, keep the filtrate, wash the solid, dry it, and obtain selenized polysaccharides. S3. Extraction of selenized protein: Dialyze the filtrate from step S2 using a dialysis bag with a pore size of 4 kDa for 6 hours, collect the dialysate and permeate, freeze-dry the dialysate to obtain selenized protein; S4. Preparation of selenized protein titanium / copper complex: 12 parts by weight of selenized protein obtained in step S3 were dissolved in 100 parts by weight of water, 0.7 parts by weight of titanium nitrate and 0.5 parts by weight of copper nitrate were added, the mixture was stirred and reacted for 25 min, dialyzed with a dialysis bag with a pore size of 4 kDa for 4 h, the dialysate was collected, and freeze-dried to obtain selenized protein titanium / copper complex. S5. Preparation of active extract: Dichloromethane was added to the permeate at a volume ratio of 1:1, and the extract was extracted. The solvent was recovered under reduced pressure, washed, and dried to obtain the active extract. S6. Preparation of purslane extract: 4 parts by weight of selenized polysaccharide obtained in step S2, 3 parts by weight of selenized protein titanium / copper complex obtained in step S4, and 1 part by weight of active extract obtained in step S5 were stirred and mixed for 10 min to obtain purslane extract.

[0044] Comparative preparation example 9 The difference from Preparation Example 3 is that selenized polysaccharide was not added in step S7.

[0045] Specifically as follows: S7. Preparation of purslane extract: 3 parts by weight of the selenized protein titanium / copper complex obtained in step S4 and 1 part by weight of the active extract liposome obtained in step S6 were stirred and mixed for 10 min to obtain purslane extract.

[0046] Comparative preparation example 10 The difference from Preparation Example 3 is that the selenized protein titanium / copper complex was not added in step S7.

[0047] Specifically as follows: S7. Preparation of purslane extract: 4 parts by weight of selenized polysaccharide obtained in step S2 and 1 part by weight of active extract liposome obtained in step S6 are stirred and mixed for 10 min to obtain purslane extract.

[0048] Comparative Preparation Example 11 The difference from Preparation Example 3 is that no active extract liposomes were added in step S7.

[0049] Specifically as follows: S7. Preparation of purslane extract: 4 parts by weight of selenized polysaccharide obtained in step S2 and 3 parts by weight of selenized protein titanium / copper complex obtained in step S4 were stirred and mixed for 10 min to obtain purslane extract.

[0050] Test Example 1 1. Effects on RAW264.7 cell proliferation RAW264.7 cells were cultured in DMEM complete medium containing 10% FBS and 1% penicillin antibiotics. Cells were trypsinized and seeded into 96-well plates at a density of 1×10⁶ cells / well.4 Cells were added to each well, with 4 replicates per group. 135 µL of cell suspension was added to each well, with 4 replicates per group. After 24 h, 15 µL of an aqueous solution of purslane extract prepared in Preparation Examples 1-6 or Control Examples 1-11 (10 mg / L) was added to each well, while 15 µL of PBS buffer was added to the control group. After incubation for 24 h, 20 µL of MTT solution (5 mg / mL) was added to each well, and the cells were cultured for another 4 h. Then, 100 µL of triple lysis buffer was added to each well, and the cells were incubated overnight at 37°C. The absorbance at 570 nm was measured using a microplate reader, and the OD was calculated. 570 Values. The results are shown in Table 1.

[0051] Table 1 As shown in the table above, the purslane extracts prepared in Examples 1-3 of this invention can significantly promote the proliferation of RAW264.7 cells without cytotoxicity.

[0052] 2. Effects of LPS on NO release induced by LPS in RAW264.7 cells RAW264.7 cells were cultured in DMEM complete medium containing 10% FBS and 1% penicillin-dextrose antibiotics. Cells were trypsinized and seeded into 96-well plates at a density of 1 × 10⁶ cells / well. 4 Cells per well, 4 replicates per group. 160 µL of cell suspension was added to each well, 4 replicates per group. After 24 h, except for the control wells, 20 µL of LPS (1 µg / mL) was added to each well. Simultaneously, 20 µL of an aqueous solution of purslane extract prepared in Preparation Examples 1-6 or Comparative Preparation Examples 1-11 (10 mg / L) was added to each well. The model group received 20 µL of PBS buffer. Compared to the model group, the control group had LPS replaced with 20 µL of PBS buffer. After 24 h of incubation, the cell supernatant was collected, and the NO content in the cell supernatant was measured according to the kit instructions. The results are shown in Table 2.

[0053] Table 2 Note: * indicates P < 0.05 compared to the model group.

[0054] As shown in the table above, the purslane extracts prepared in Examples 1-3 of this invention have significant anti-inflammatory activity in cells, and the NO release is significantly lower than that in the model group.

[0055] Test Example 2 1. Determination of hydroxyl radical scavenging ability Add 0.2 mL of 20 mmol / L 2-D-deoxyribose solution, 0.2 mL of 10 mmol / L FeSO4-EDTA mixture, and 0.2 mL of aqueous solution of purslane extract prepared in Preparation Examples 1-6 or Comparative Preparation Examples 1-11 to test tubes, respectively. Add PBS buffer to a final volume of 1.8 mL, and finally add 0.2 mL of 10 mmol / L hydrogen peroxide. Heat the mixture in a water bath at 37°C for 1 h. Use PBS buffer instead of the sample solution as a blank control. Use benzoic acid (0.5 mg / mL) and ascorbic acid (0.5 mg / mL) as positive controls. Stop the reaction by adding 1 mL of 10% trichloroacetic acid, then add 1 mL of 1% thiobarbituric acid. Mix well and heat in a boiling water bath for 10 min. After cooling, centrifuge and collect the supernatant. Measure the absorbance at 532 nm.

[0056] Hydroxyl radical scavenging rate (%) = (A 空白 -A 样品 ) / A 空白 ×100%.

[0057] 2. Effects on hydrogen peroxide-induced lipid peroxidation Mouse liver tissue was washed with cold physiological saline and homogenized under ice to prepare a 1 wt% suspension. 1 mL of the suspension, 0.1 mL of an aqueous solution of purslane extract prepared in Examples 1-6 or Comparative Examples 1-11 (concentration 0.5 mg / mL), 0.1 mL of 6 mmol / L FeSO4 solution, and 0.1 mL of 60 mmol / L H2O2 solution were added. For the blank control group, 0.1 mL of PBS buffer was added. After incubation at 37°C for 1 h, 1 mL of 15 wt% trichloroacetic acid solution was added to terminate the reaction. The supernatant was collected by centrifugation, and 1 mL of 0.67% thiobarbituric acid solution was added. The mixture was then boiled in water for 15 min, cooled under running water, and the absorbance was measured at 532 nm. Benzoic acid (1 mg / mL) and ascorbic acid (1 mg / mL) were used as positive controls.

[0058] Lipid peroxidation inhibition rate (%) = Absorbance of blank well - Absorbance of sample well / Absorbance of blank well × 100%.

[0059] The results are shown in Table 3.

[0060] Table 3 As can be seen from the table above, the purslane extracts prepared in Examples 1-3 of this invention have good antioxidant activity.

[0061] Preparation Example 7: Preparation of Permeation-Enhancing Compounds The method is as follows: T1. 0.1 mol d 4-Terpineol and 0.13 mol of salicylic acid were dissolved in 200 mL of toluene, and 1 mL of concentrated sulfuric acid was added. The mixture was heated under reflux for 6 h. After the reaction was completed, the reaction solution was washed three times with saturated sodium carbonate and once with water. The oil layer was collected, toluene was recovered under reduced pressure, dried, filtered, and purified by column chromatography (ethyl acetate:petroleum ether volume ratio 1:50) to obtain salicylic acid. d -4-terpineol ester; ESI-MS calculated value: C 17 H 23 O3(M+H) + 275.16, measured value: 275.2, yield: 56.2%. NMR results: 1 HNMR (300MHz, CDCl3) δ7.82 (d, J =6.7Hz, 1H), 7.22 (m, 1H), 6.90 (m, 1H), 6.81 (d, J =5.9Hz, 1H), 5.32 (m, 1H), 5.0 (br, 1H), 2.45 (s, 1H), 2.32 (s, 2H), 2.01 (t, 2H), 1.95 (t, 2H), 1.72 (s, 3H), 0.97 (d, 6H).

[0062] T2. Dissolve 0.1 mol n-dodecyl alcohol and 0.13 mol 2-chloropropionic acid in 200 mL toluene, add 0.005 mol ferric chloride, and heat under reflux for 4 h. After the reaction is complete, wash the reaction solution three times with saturated sodium carbonate and once with water. Collect the oil layer, recover toluene under reduced pressure, dry, filter, and purify by column chromatography (ethyl acetate:petroleum ether volume ratio 1:20) to obtain dodecyl 2-chloropropionic acid ester; ESI-MS calculated value: C 15 H 30 ClO2(M+H) + 277.19, measured value: 277.2, yield: 87.4%. NMR results: 1 H NMR (300MHz, CDCl3) δ4.45 (q, 1H), 4.05 (t, 2H), 1.77 (d, 3H), 1.57 (m, 2H), 1.35 (m, 2H), 1.2-1.3 (m, 16H), 0.92 (t, 3H).

[0063] T3. Mix 0.12 mol of dodecanol 2-chloropropionate and 0.1 mol of salicylic acid. d-4-terpineol ester and 0.3 mol triethylamine were added to 200 mL acetonitrile, heated to 50 °C, and stirred for 5 h. The reaction solution was washed twice with saturated sodium carbonate and once with water. The oil layer was collected, dried, filtered, and the solvent was removed under reduced pressure. The product was purified by column chromatography (ethyl acetate and petroleum ether, volume ratio 1:15). ESI-MS calculated value: C 32 H 51 O5(M+H) + 515.37, measured value: 515.4, yield: 84.5%. NMR results: 1 HNMR (300MHz, CDCl3) δ7.92 (d, J =7.2Hz, 1H), 7.37 (m, 1H), 6.91 (m, 1H), 6.84 (d, J =6.8Hz, 1H), 5.32 (m, 1H), 4.29 (s, 1H), 4.02 (t, 2H), 2.37 (s, 1H), 2.32 (s, 2H), 2.05 (t, 2H), 1.94 (t, 2 H), 1.74 (s, 3H), 1.64 (d, 3H), 1.54 (m, 2H), 1.33 (t, 2H), 1.24-1.3 (m, 16H), 1.02 (d, 6H), 0.94 (t, 3H).

[0064] Example 1 This embodiment provides a method for preparing a skin conditioning agent, comprising the following steps: mixing 5 parts by weight of purslane extract obtained in Preparation Example 1, 0.5 parts by weight of a penetration enhancer composition, 0.2 parts by weight of peptides, sodium hyaluronate, 0.7 parts by weight of ascorbic acid, 10 parts by weight of ethanol, and 5 parts by weight of water for 20 minutes to obtain the skin conditioning agent.

[0065] The permeation enhancer composition comprises menthol and the permeation enhancer compound prepared in Preparation Example 7, in a mass ratio of 1-2:5.

[0066] Example 2 This embodiment provides a method for preparing a skin conditioning agent, comprising the following steps: mixing 7 parts by weight of purslane extract obtained in Preparation Example 2, 1 part by weight of a penetration enhancer composition, 0.4 parts by weight of peptide, sodium hyaluronate, 1.2 parts by weight of ascorbic acid, 12 parts by weight of ethanol, and 7 parts by weight of water for 20 minutes to obtain the skin conditioning agent.

[0067] The permeation enhancer composition comprises menthol and the permeation enhancer compound prepared in Example 7, in a mass ratio of 2:5.

[0068] Example 3 This embodiment provides a method for preparing a skin conditioning agent, comprising the following steps: mixing 6 parts by weight of purslane extract obtained in Preparation Example 3, 0.7 parts by weight of a penetration enhancer composition, 0.3 parts by weight of peptide, sodium hyaluronate, 1 part by weight of ascorbic acid, 11 parts by weight of ethanol, and 6 parts by weight of water for 20 minutes to obtain the skin conditioning agent.

[0069] The permeation enhancer composition comprises menthol and the permeation enhancer compound obtained in Preparation Example 7, in a mass ratio of 1.5:5.

[0070] Examples 4-6 and Comparative Examples 1-11 The difference from Example 3 is that the purslane extract was prepared by Preparation Examples 4-6 or Comparative Preparation Examples 1-11.

[0071] Comparative Example 12 The difference compared to Example 3 is that purslane extract was not added.

[0072] Comparative Example 13 The difference compared to Example 3 is that the penetration enhancer composition is a single menthol.

[0073] Comparative Example 14 The difference from Example 3 is that the permeation enhancer composition is a single permeation enhancer compound obtained in Preparation Example 7.

[0074] Comparative Example 15 The difference compared to Example 3 is that no penetration enhancer composition was added.

[0075] Test Example 3 One hundred healthy female volunteers, aged 35-55 years, were selected. Exclusion criteria for volunteers met the inclusion and exclusion criteria of the "Diagnostic Criteria and Treatment Principles for Cosmetic Contact Dermatitis". After cleansing their faces morning and evening, participants applied 0.5 mL of the skin conditioning agent solution prepared in Examples 1-6 and Comparative Examples 1-15 of this invention each time, massaging and patting until absorbed. Other skincare products were discontinued during the experiment. The test areas were the left and right cheeks of the face. Before testing, the test areas were washed with water and kept still for 30 minutes in a constant temperature and humidity environment of 25±0.5℃ and 55±5%RH before testing. The experiment lasted 28 days, with skin tests conducted on day 0 and day 29. The results are shown in Table 4.

[0076] Rate of change (%) = (Test value on day 29 - Initial value on day 0) / Initial value on day 0 × 100% Table 4 As can be seen from the table above, the skin conditioning agents prepared in Examples 1-3 of the present invention have good moisturizing, anti-wrinkle and skin elasticity-restoring effects.

[0077] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A skin conditioning agent, characterized in that, It is prepared from the following raw materials in parts by weight: 5-7 parts of purslane extract, 0.5-1 parts of penetration enhancer composition, 0.2-0.4 parts of peptide, 1-2 parts of sodium hyaluronate, 0.7-1.2 parts of ascorbic acid, 10-12 parts of ethanol, and 5-7 parts of water; The penetration enhancer composition comprises menthol and a penetration enhancer compound in a mass ratio of 1-2:5, and the structural formula of the penetration enhancer compound is shown in Formula I: Formula I; The preparation method of the purslane extract is as follows: S1. Enzymatic fermentation: Wash, dry, and crush purslane, add it to water, heat to boiling to extract, then cool to room temperature, add compound enzyme, enzymatic hydrolysis, sterilize, inoculate with selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum seed liquid, enzymatic fermentation culture, filter, and collect filtrate. S2. Extraction of selenized polysaccharides: Ethanol is added to the filtrate from step S1 to precipitate the polysaccharides, which are then filtered and the solid is collected. The filtrate is retained, and the solid is washed and dried to obtain selenized polysaccharides. S3. Extraction of selenized protein: Dialyze the filtrate from step S2, collect the dialysate and permeate, freeze-dry the dialysate to obtain selenized protein; S4. Preparation of selenized protein titanium / copper complex: The selenized protein obtained in step S3 was dissolved in water, titanium salt and copper salt were added, the mixture was stirred and reacted, dialyzed, the dialysate was collected, and freeze-dried to obtain the selenized protein titanium / copper complex. S5. Preparation of active extract: Dichloromethane is added to the permeate, extraction is performed, solvent is recovered under reduced pressure, washing is performed, and drying is carried out to obtain the active extract; S6. Preparation of active extract liposomes: The active extract obtained in step S5, soybean lecithin, propylene glycol and polyethylene glycol are mixed and dissolved, water is added, ultrasonic treatment is performed, filtration is carried out, and freeze drying is performed to obtain active extract liposomes. S7. Preparation of purslane extract: The selenized polysaccharide obtained in step S2, the selenized protein titanium / copper complex obtained in step S4, and the active extract liposome obtained in step S6 are mixed evenly to obtain purslane extract.

2. The skin conditioning agent according to claim 1, characterized in that, In step S1, the solid-liquid ratio of purslane to water is 1:5-7 g / mL, the extraction time by heating and boiling is 2-4 h, the compound enzyme includes cellulase, hemicellulase, and pectinase in a mass ratio of 4-7:1-2:2-4, the amount of the compound enzyme added is 2-4 wt% of the total mass of the system, the enzymatic hydrolysis temperature is 45-50℃, and the time is 1-3 h, and the bacterial count of the selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum, and Bifidobacterium longum seed solution is 10. 8 -10 9 The inoculation amounts of the selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum, and Bifidobacterium longum seed culture were 2-4 v / v%, 0.5-1 v / v%, 0.2-0.5 v / v%, and 0.3-0.5 v / v, respectively. The enzymatic fermentation conditions were 40-45℃, 100-200 r / min, and 36-48 h. In step S2, ethanol was added until the ethanol content in the system was 80-85 wt%, and the precipitation time was 3-5 h.

3. The skin conditioning agent according to claim 1, characterized in that, In step S3, the dialysis bag used for dialysis has a pore size of 3k-5kDa, and the dialysis time is 5-7h. In step S4, the mass ratio of selenized protein, titanium salt, and copper salt is 10-15:0.5-1:0.3-0.7, the titanium salt is selected from at least one of titanium chloride, titanium sulfate, and titanium nitrate, and the copper salt is selected from at least one of copper chloride, copper sulfate, and copper nitrate. The stirring reaction time is 20-30min, the dialysis bag used for dialysis has a pore size of 3k-5kDa, and the dialysis time is 3-5h.

4. The skin conditioning agent according to claim 1, characterized in that, In step S5, the volume ratio of the permeate to dichloromethane is 1:0.7-1.2; in step S6, the mass ratio of the active extract, soybean lecithin, propylene glycol, polyethylene glycol, and water is 0.7-1.2:7-12:2-4:6-12:100; the ultrasonic treatment power is 500-700W, and the time is 10-15min; the filtration uses a 0.22μm microporous membrane; in step S7, the mass ratio of the selenized polysaccharide, selenized protein titanium / copper complex, and active extract liposomes is 3-5:2-4:0.7-1.

2.

5. The skin conditioning agent according to claim 1, characterized in that, The specific method for preparing the purslane extract is as follows: S1. Enzymatic fermentation: Wash, dry, and crush purslane, add it to water, the solid-liquid ratio of purslane to water is 1:5-7 g / mL, heat to boiling and extract for 2-4 h, then cool to room temperature, add compound enzyme, the amount added is 2-4 wt% of the total mass of the system, enzymatic hydrolysis at 45-50℃ for 1-3 h, sterilize, inoculate with selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum seed liquid, the inoculation amount is 2-4 v / v%, 0.5-1 v / v%, 0.2-0.5 v / v% and 0.3-0.5 v / v%, respectively, at 40-45℃, 100-200 r / min, enzymatic fermentation for 36-48 h, filter, and collect the filtrate; The complex enzyme includes cellulase, hemicellulase, and pectinase in a mass ratio of 4-7:1-2:2-4. The bacterial counts of the selenium-enriched yeast, Bacillus subtilis, Lactobacillus plantarum, and Bifidobacterium longum seed solutions were 10. 8 -10 9 cfu / mL; S2. Extraction of selenized polysaccharides: Add ethanol to the filtrate in step S1 until the ethanol content in the system is 80-85 wt%, precipitate for 3-5 hours, filter, collect the solid, keep the filtrate, wash the solid, dry it, and obtain selenized polysaccharides. S3. Extraction of selenized protein: Dialyze the filtrate from step S2 using a dialysis bag with a pore size of 3k-5kDa for 5-7 hours, collect the dialysate and permeate, freeze-dry the dialysate to obtain selenized protein; S4. Preparation of selenized protein titanium / copper complex: Dissolve 10-15 parts by weight of the selenized protein obtained in step S3 in 100 parts by weight of water, add 0.5-1 parts by weight of titanium salt and 0.3-0.7 parts by weight of copper salt, stir and react for 20-30 min, dialyze with a dialysis bag with a pore size of 3k-5kDa for 3-5 h, collect the dialysate, freeze dry, and obtain selenized protein titanium / copper complex; S5. Preparation of active extract: Dichloromethane was added to the permeate at a volume ratio of 1:0.7-1.2, and the extract was extracted. The solvent was recovered under reduced pressure, washed, and dried to obtain the active extract. S6. Preparation of active extract liposomes: Mix and dissolve 0.7-1.2 parts by weight of the active extract obtained in step S5, 7-12 parts by weight of soybean lecithin, 2-4 parts by weight of propylene glycol, and 6-12 parts by weight of polyethylene glycol, add 100 parts by weight of water, sonicate at 500-700W for 10-15 min, filter with a 0.22μm microporous membrane, and freeze-dry to obtain active extract liposomes; S7. Preparation of purslane extract: Mix 3-5 parts by weight of the selenized polysaccharide obtained in step S2, 2-4 parts by weight of the selenized protein titanium / copper complex obtained in step S4, and 0.7-1.2 parts by weight of the active extract liposome obtained in step S6 evenly to obtain purslane extract.

6. The skin conditioning agent according to claim 1, characterized in that, The method for preparing the permeation-enhancing compound is as follows: T1. will d -4-terpineol reacts with salicylic acid to produce salicylic acid. d -4-terpineol ester, with the following structure: ; T2. The dodecyl ester of 2-chloropropionic acid was prepared by reacting n-dodecyl alcohol with 2-chloropropionic acid, with the following structure: ; T3. Dodecyl 2-chloropropionate and salicylic acid d The product was obtained by reacting -4-terpineol alcohol ester.

7. The skin conditioning agent according to claim 6, characterized in that, The steps described in step T1 d The molar ratio of -4-terpineol to salicylic acid is 1:1.2-1.4, and concentrated sulfuric acid is added as a catalyst. The reaction temperature is 110-120℃, and the time is 5-7 h. In step T2, the molar ratio of n-dodecyl alcohol to 2-chloropropionic acid is 1:1.2-1.4, and ferric chloride is added as a catalyst. The reaction temperature is 100-120℃, and the time is 3-5 h. In step T3, dodecyl 2-chloropropionic acid ester and salicylic acid... d The molar ratio of 4-terpineol ester is 1.1-1.3:1, and a base is added, wherein the base is selected from at least one of triethylamine, NaOH, and KOH, and the reaction temperature is 45-50℃ for 4-6 hours.

8. A method for preparing a skin conditioning agent as described in any one of claims 1-7, characterized in that, Includes the following steps: A skin conditioning agent is prepared by uniformly mixing purslane extract, a penetration enhancer composition, peptides, sodium hyaluronate, ascorbic acid, ethanol, and water.

9. The use of a skin conditioning agent as described in any one of claims 1-7 in the preparation of whitening, anti-aging, anti-inflammatory, anti-allergic, antioxidant, moisturizing, and anti-wrinkle cosmetics.

Images