Probe for single cell ribosome imaging and tracking

A cycloheximide-fluorescent compound linked at the C13 position selectively binds to the 60S ribosomal subunit, addressing the lack of live-cell ribosome imaging probes and enabling effective tracking and sorting in eukaryotic cells.

WO2026121964A1 Publication Date: 2026-06-11STICHTING RADBOUD UNIVERSITEIT
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Patent Information

Application Number
PCT/NL2025/150006
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-12-03
Filing Date
2025-12-03
Publication Date
2026-06-11

AI Technical Summary

Technical Problem

There is a lack of selective probes for live-cell ribosome imaging and tracking in eukaryotic cells, particularly mammalian cells, hindering single-cell studies of ribosomal movement.

Method used

A compound comprising a cycloheximide moiety linked to a fluorescent moiety through the C13 position, which selectively binds to the 60S ribosomal subunit, enabling live-cell imaging and tracking of ribosomes in eukaryotic cells.

Benefits of technology

Enables visualization and tracking of ribosomes and their movement in individual live cells, allowing for methods like fluorescent microscopy and flow cytometry, and sorting of cells based on ribosome content.

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Abstract

This invention pertains in general to ribosome imaging, in particular ribosome imaging in live cells. In particular, the invention relates to a compound (probe) that selectively binds with eukaryotic ribosomes, such as mammalian, human and / or mouse ribosomes. Also provided is for a method of preparing the compound and for a method for detecting a eukaryotic, preferably mammalian, preferably human and / or mouse, 60S ribosomal subunit or ribosome using the compound.
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