Application of isoeugenol in the preparation of inhibitors for preventing and controlling pear fire blight disease and preparation of isoeugenol

Abstract

The present application discloses an application of isoeugenol in the preparation of an inhibitor for preventing and controlling pear fire blight disease and the preparation of isoeugenol, which relates to the field of biotechnology. By preparing a crude extract of Streptomyces sp.D67 to verify its activity against purple bacillus and amylovora pathogens, and verifying the active substances of the metabolites, it was determined that the metabolite isoeugenol has an inhibitory effect on amylovora pathogens. Isoeugenol is used in the preparation of inhibitors for preventing and controlling amylovora blight, with an effective concentration of 0.125mg / mL-0.5mg / mL. It has an inhibition rate of 44% on biofilm formation and 27% on extracellular polysaccharide formation. Isoeugenol can effectively inhibit the expression of virulence factors of amylovora blight pathogens, thereby reducing the drug resistance of amylovora blight pathogens and alleviating the occurrence of plant diseases and infection of plants. Spread effect.