Bioelectrochemical sensor used for detecting hydrogen peroxide and manufacturing method thereof

By constructing a peptide matrix on the surface of a gold electrode and utilizing the specific binding of peptides to HRP, the problem of directional immobilization of enzymes on the electrode surface was solved, thereby improving the sensitivity of the enzyme biosensor and the detection performance of hydrogen peroxide.

CN102654475AInactive Publication Date: 2012-09-05SHANGHAI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI UNIV
Filing Date
2012-03-23
Publication Date
2012-09-05
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

In existing enzyme biosensors, the problem of directional immobilization of enzymes on the electrode surface leads to slow electron transfer rates, affecting detection performance.

Method used

A specific sequence of peptide matrix is ​​constructed on the surface of a gold electrode. Through the specific binding of peptides to horseradish peroxidase (HRP), the enzyme is directionally immobilized, promoting the exposure of the enzyme's active site and improving electron transfer efficiency.

Benefits of technology

This improved the sensitivity of the enzyme biosensor and the sensitivity of hydrogen peroxide detection, achieving highly sensitive detection of hydrogen peroxide.

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Abstract

The invention relates to a bioelectrochemical sensor used for detecting hydrogen peroxide and a manufacturing method of the bioelectrochemical sensor. The novel bioelectrochemical sensor is a sensor with a three-electrode system, wherein the counter electrode is a platinum electrode, the reference electrode is saturated calomel electrode and the working electrode is gold electrode. The sensor is based on orientation combination of a polypeptide horse radish peroxidase with characteristic sequence, the promotion effect of the polypeptide horse radish peroxidase on the activity of the enzyme and the characteristic of high sensitivity of electrochemical detection methods. The sensitivity of hydrogen peroxide sensors is effectively improved and satisfactory results are obtained.
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