Chemiluminescent immunoassay reaction disk and analyzer with multiple reaction modes

Abstract

The present invention relates to the technical field of manufacturing fully-automatic chemiluminescence immunoassay instruments, and more specifically to a chemiluminescence immunoassay reaction disk and analyzer with multiple reaction modes that are rationally structured, reliable in operation, and capable of meeting multi-mode detection requirements. The disk comprises an annular disk body with an annular track groove formed thereon. The disk body is characterized in that an annular incubation track and an annular functional track are respectively formed thereon, and functional positions for adding samples, adding reagents, mixing, washing, measuring, or unloading are respectively provided on the annular functional track. The annular functional track is also provided with at least one lane changing position, and a gripping mechanism for switching the current reaction cup between the functional track and the incubation track is provided on the lane changing position. Compared with the prior art, the present invention has significant advantages such as rational structure, reliable operation, wide applicability, and high detection efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of fully automatic chemiluminescence immunoassay manufacturing, and in particular to a chemiluminescence immunoassay reaction disk and analyzer with multiple reaction modes that are reasonable in structure, reliable in operation, and capable of meeting multi-mode detection requirements. Background Art

[0002] Currently, fully automatic chemiluminescence immunoassays have increasingly higher requirements for the accuracy and stability of test results. Since their test results play a very important role in guiding medical treatment and surgical rescue, the detection speed directly affects the efficiency of clinical treatment. Although such equipment at this stage has achieved the integration of detection components, the detection mode is single. This is mainly because the incubation mechanism, washing mechanism and measurement mechanism of the existing equipment are distributed on a single-track turntable, resulting in each test sample needing to pass through all the mechanisms to complete the test. For different testing requirements, there are often differences in the number of reagent additions and the number of sample washings. For traditional equipment, the detection requirements that are different from the module setting mode on the single-track turntable require multiple rotations of the turntable to complete, resulting in long detection time, inaccurate test results and other problems. Summary of the Invention

[0003] In view of the shortcomings and deficiencies in the prior art, the present invention proposes a chemiluminescent immunoassay reaction disk and analyzer with multiple reaction modes, which have a reasonable structure, reliable operation, and can meet the needs of multi-mode detection.

[0004] The present invention can be achieved by the following measures:

[0005] A chemiluminescent immunoassay reaction disk with multiple reaction modes is provided with an annular disk body with an annular track groove provided on the disk body. The invention is characterized in that an annular incubation track and an annular functional track are respectively provided on the disk body, and functional positions for adding samples, adding reagents, mixing, washing, measuring or unloading are respectively provided on the annular functional track. The annular functional track is also provided with at least one lane changing position, and a gripping mechanism is provided on the lane changing position for realizing the switching of the current reaction cup to the functional track or the incubation track.

[0006] The annular incubation track and the annular functional track of the present invention are concentrically arranged. Preferably, the annular functional track is located outside the annular incubation track, so as to facilitate the arrangement of various functional components outside the tray body.

[0007] The lower part of the annular incubation track of the present invention is provided with a heating layer, and the heating layer is preferably composed of 7 heating zones, so as to provide different heating environments in a targeted manner.

[0008] The annular incubation track of the present invention is provided with an incubation transmission rack, and the annular functional track is provided with a functional transmission rack. Both the incubation transmission rack and the functional transmission rack are annular, and both have two or more reaction cup slots distributed at equal intervals, wherein the reaction cup slots are located above the track groove. The incubation transmission rack and the functional transmission rack are driven by an electric motor through a gear transmission mechanism to rotate around the center of the turntable, driving the reaction cups to move along the incubation track and the functional track respectively.

[0009] The function transmission rack described in the present invention can be operated using the following structure: continuous serrations that mesh with the function motor gear are set on the side of the function transmission rack, and the output shaft of the function motor drives the function motor gear to rotate, thereby continuously driving the function transmission rack to operate; further, at least one function transmission auxiliary gear that meshes with the outer ring serrations of the function transmission rack is set on the outer side of the annular disk body, and the function transmission auxiliary gear is fixed to the outside of the turntable via an axis. Preferably, two or more symmetrically distributed function transmission gears are set to correct the operation of the annular function transmission rack.

[0010] The incubation transmission rack described in the present invention can be driven to rotate by a separate incubation transmission motor, or it can be driven to rotate by a functional motor through a gear transmission mechanism by arranging continuous serrations on the outer ring of the incubation transmission rack; further, at least one incubation transmission auxiliary gear is arranged on the inner side of the incubation transmission rack, and the incubation transmission auxiliary gear is engaged with the continuous serrations on the inner ring of the incubation transmission rack, and is fixed to the annular turntable via the gear shaft, for correcting the operation of the incubation transmission rack.

[0011] The incubation transmission rack of the present invention is provided with a position sensor for detecting its motion position information, and the functional transmission rack is also provided with a position sensor for detecting the motion information of the functional rack.

[0012] The annular turntable of the present invention is provided with two sets of lane-changing gripper mechanisms, which are respectively used to grab the reaction cups on the incubation track to the functional track, and to grab the reaction cups on the functional track to the incubation track.

[0013] The present invention preferably arranges a loading area, a first sample adding area, a first mixing area, a first lane changing grab area, a first washing area, a second sample adding area, a second mixing area, a second lane changing grab area, a second washing area, a third mixing area, a measuring area, and an unloading area on the annular turntable in sequence.

[0014] The annular cover plate is buckled on the upper part of the annular turntable of the present invention.

[0015] The present invention also provides a chemiluminescence immunoassay analyzer with multiple reaction modes, which is characterized by being provided with the above-mentioned annular turntable.

[0016] The measuring area of ​​the present invention is provided with a measuring mechanism; the washing area is provided with a washing mechanism; the mixing area is provided with a mixing mechanism; and the unloading area is provided with a consumables unloading mechanism.

[0017] The present invention sets up independent incubation tracks and functional tracks, so that the entire device can meet the needs of multiple reaction modes, wherein the present solution can meet the needs of at least 7 reaction modes, namely: loading reaction cup - adding sample - adding reagent 1 - mixing 1 - changing lane 1 - incubating - changing lane 2 - washing 2 - mixing 3 - measuring - unloading; (2) loading reaction cup - adding sample - adding reagent 1 - mixing 1 - changing lane 1 - incubating - changing lane 1 - washing 1 - washing 2 - mixing 3 - measuring - unloading (3) loading reaction cup - adding sample - adding reagent 1 - mixing 1 - changing lane 1 - incubating - changing lane 1 - reagent 2 - washing 2 - mixing 3 - measuring - unloading; (4) loading reaction cup - adding sample - adding reagent 1 -Mix 1 - Change lane 1 - Incubate - Change lane 1 - Wash 1 - Reagent 2 - Wash 2 - Mix 3 - Measure - Unload (5) Load the reaction cup - Add sample - Add reagent 1 - Mix 1 - Change lane 1 - Incubate - Change lane 1 - Wash 1 - Change lane 2 - Incubate - Change lane 2 - Wash 2 - Mix 3 - Measure - Unload; (6) Load the reaction cup - Add sample - Add reagent 1 - Mix 1 - Change lane 1 - Incubate - Change lane 1 - Reagent 2 - Change lane 2 - Incubate - Change lane 2 - Wash 2 - Mix 3 - Measure - Unload; (7) Load the reaction cup - Add sample - Add reagent 1 - Mix 1 - Change lane 1 - Incubate - Change lane 1 - Wash 1 - Reagent 2 - Change lane 2 - Incubate - Change lane 2 - Wash 2 - Mix 3 - Measure - Unload.

[0018] Compared with the prior art, the present invention has significant advantages such as reasonable structure, reliable operation, wide applicability and high detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Attachment Figure 1 It is a structural schematic diagram of the annular turntable in the present invention.

[0020] Attachment Figure 2 It is attached Figure 1 A partial enlarged view of .

[0021] Attachment Figure 3 It is a structural diagram of an embodiment of the present invention.

[0022] Attachment Figure 4 Schematic diagram of the transmission rack in the present invention.

[0023] Attachment Figure 5 This is a schematic diagram of a driving method of the incubation transmission rack in the present invention.

[0024] Attachment Figure 6 It is a schematic diagram of a correction method for the incubation transmission rack in the present invention.

[0025] Attachment Figure 7 This is a schematic diagram of a heating structure of the incubation track in the present invention, wherein Figure 7 (a) is a schematic diagram of the division of the heating zone. Figure 7 (b) Schematic diagram of the heating layer structure.

[0026] Attachment Figure 8 It is a structural diagram of the functional transmission rack loading functional position in the present invention.

[0027] Attachment Figure 9 This is a schematic diagram of the structure of the measuring position in the present invention, Figure 9 (a) is the cross-sectional view of the measurement position, Figure 9 (b) is a top view of the measurement position.

[0028] Attachment Figure 10 It is a structural diagram of the baffle bottom plate.

[0029] Attachment Figure 11 It is a structural diagram of the measuring mechanism.

[0030] Attachment Figure 12 This is the structure and use status diagram of the unloading mechanism in the present invention, wherein Figure 12 (a) is a schematic diagram of the unloading mechanism structure, Figure 12 (b) is a schematic diagram of the unloading paddle in the initial unloading state, with Figure 12 (c) is a structural diagram of the unloading paddle during unloading.

[0031] Attachment Figure 13 This is a schematic diagram of the structure of Example 2 of the present invention, Figure 13 (a) is a schematic structural diagram of the washing mechanism in Example 2, 13 (b) is a structural diagram of the liquid injection and liquid aspiration combination needle, and 13 (c) is a schematic structural diagram of the magnet mounting plate.

[0032] Attachment Figure 14 is a schematic structural diagram of the washing mechanism in Example 5, Figure 14 (a) is a diagram showing the washing mechanism in use in Example 5. Figure 14 (b) is a schematic diagram of the magnet distribution of each functional position in the washing area in Example 5, Figure 14 (c) is a top view of the functional positions of the washing area in Example 5.

[0033] Attachment Figure 15 The structural diagram of the mixing mechanism in Example 3 is shown in FIG. Figure 15 (a) is a schematic diagram of the structure of the mixing mechanism, Figure 15 (b) is the assembly diagram of the motor and the rotating shaft in the mixing mechanism; Figure 15 (c) is another structural diagram of the mixing mechanism; Figure 15 (d) is a schematic diagram of the structure of the mixing sleeve in the mixing mechanism. Figure 15 (e) is the cross-sectional view of 15 (d) along the AA direction.

[0034] Figure 1: tray 1, incubation track 2, functional track 3, external temperature zone 4, internal temperature zone 5, drain 6, incubation transmission rack 7, functional transmission rack 8, reaction cup slot 9, functional motor gear 10, functional motor 11, functional transmission auxiliary gear 12, position sensor 13, incubation motor 14, baffle lifting slot 15, cup position to be tested 16, test cup position 17, tested cup position 18, photon counter 19, waste liquid position 20, incubation transmission auxiliary gear 21, position sensor 2 ... Auxiliary gear 21, lane change gripper mechanism 22, loading area 23, first sample adding area 24, first lane change gripper area 25, first washing area 26, second sample adding area 27, second mixing area 28, second lane change gripper area 29, second washing area 30, third mixing area 31, measuring area 32, unloading area 33, first mixing area 34, loading position 35, loading position sensor 36, light shielding bottom plate 37, waste liquid cup position 38, annular cover plate 39, linear lifting motor 40, Linear lifting motor fixed base plate 41, fixed side plate 42, light baffle position baffle 43, position sensor 44, left end baffle 45, right end baffle 46, left detection cup position baffle 47, right detection cup position baffle 48, annular boss 49, liquid storage tank 50, photon counter 51, photon counter fixed plate 52, magnet mounting plate 53, magnet mounting slot 54, magnet 55, pre-adsorption magnet mounting slot 56, lifting needle holder 57, liquid injection and liquid aspiration combination needle 58, liquid aspiration needle 59, injection needle 60, reaction cup 61, bottom plate 62, vertical plate 63, pressure plate 64, split teeth 65, pre-adsorption area 66, adsorption area 67, non-filling area 68, mixing sleeve 69, rotating shaft 70, latch 71, spiral groove hole 72, eccentric hole 73, motor 74, inner sleeve 75, outer sleeve 76, base 77, mixing sleeve bracket 78, vertical plate 79, guide groove 80, position sensor 81, support ring 82, water guide hole 83, magnet 84. DETAILED DESCRIPTION

[0035] The present invention will be further described below with reference to the accompanying drawings and examples.

[0036] As attached Figure 1 and attached Figure 2As shown, the present invention proposes a chemiluminescent immunoassay reaction disk with multiple reaction modes, which is provided with an annular disk body 1, an annular track groove is provided on the disk body 1, an annular incubation track 2 and an annular functional track 3 are respectively provided on the disk body, and functional positions for adding samples or adding reagents or mixing or washing or measuring or unloading are respectively provided on the annular functional track 3, and at least one lane changing position is provided on the lane changing position, and a gripping mechanism for realizing the switching of the current reaction cup to the functional track 3 or the incubation track 2 is provided; the annular incubation track 2 and the annular functional track 3 are concentrically arranged, and preferably the annular functional track 3 is located outside the annular incubation track 2, so as to facilitate the arrangement of various functional components on the outside of the disk body 1; as shown in the attached figure Figure 7 As shown, the bottom of the annular incubation track 2 is provided with a bottom heating layer Figure 7 (b) The heating zone is preferably composed of 7 heating zones, which can be divided into an outer temperature zone 4 and an inner temperature zone 5, and a drain outlet 6 is provided; thereby providing different heating environments in a targeted manner.

[0037] As attached Figure 4 、 5 As shown in Figures 6 and 7, the annular incubation track 2 of the present invention is provided with an incubation transmission rack 7, and the annular functional track 3 is provided with a functional transmission rack 8. Both the incubation transmission rack 7 and the functional transmission rack 8 are annular, and both have two or more reaction cup slots 9 distributed at equal intervals, wherein the reaction cup slots 9 are located above the track groove. The incubation transmission rack 7 and the functional transmission rack 8 are driven by an electric motor through a gear transmission mechanism to rotate around the center of the disk body 1, driving the reaction cups to move along the incubation track 2 and the functional track 3 respectively.

[0038] As attached Figure 8 As shown, the function transmission rack 8 of the present invention can be operated by the following structure: continuous serrations are set on the side of the function transmission rack 8 and meshed with the function motor gear 10, and the output shaft of the function motor 11 drives the function motor gear 10 to rotate, thereby continuously driving the function transmission rack 8 to operate; further, at least one function transmission auxiliary gear 12 is set on the outside of the annular disk body 1 and meshed with the outer ring serrations of the function transmission rack 8, and the function transmission auxiliary gear 12 is fixed to the outside of the disk body 1 through the shaft, and preferably two or more symmetrically distributed function transmission gears 12 are set to correct the operation of the annular function transmission rack 8.

[0039] like Figure 5As shown, the incubation transmission rack 7 described in the present invention can be driven to rotate by a separate incubation transmission motor 14, or continuous serrations can be set on the outer ring of the incubation transmission rack 7, and the rack can be driven to rotate by a functional motor through a gear transmission mechanism; further, at least one incubation transmission auxiliary gear 21 is set on the inner side of the incubation transmission rack 7, and the incubation transmission auxiliary gear 21 is engaged with the continuous serrations on the inner ring of the incubation transmission rack 7, and is fixed to the annular turntable via a gear shaft, so as to correct the operation of the incubation transmission rack 7.

[0040] The incubation transmission rack 7 of the present invention is provided with a position sensor 13 for detecting its motion position information, and the functional transmission rack 8 is also provided with a position sensor 13 for detecting the motion information of the functional rack.

[0041] The annular disc 1 of the present invention is provided with two sets of lane-changing gripper mechanisms 22 , which are respectively used to grab the cuvette on the incubation track 2 to the functional track 3 , and to grab the cuvette on the functional track 3 to the incubation track 2 .

[0042] The present invention preferably sets up the loading area 23, the first sample adding area 24, the first mixing area 34, the first lane changing gripping area 25, the first washing area 26, the second sample adding area 27, the second mixing area 28, the second lane changing gripping area 29, the second washing area 30, the third mixing area 31, the measuring area 32, and the unloading area 33 in sequence on the annular disk 1, wherein as shown in the attached figure, Figure 8 As shown, the cuvette slot corresponding to the loading area 23 is a loading position 35 , on which a frame-type loading auxiliary support and a loading position sensor 36 are provided.

[0043] The annular disc body 1 of the present invention is buckled with an annular cover plate 39 .

[0044] The present invention also proposes a chemiluminescence immunoassay analyzer with multiple reaction modes, which is characterized by being provided with the annular disc 1 mentioned above.

[0045] The measuring area of ​​the present invention is provided with a measuring mechanism; the washing area is provided with a washing mechanism; the mixing area is provided with a mixing mechanism; and the unloading area is provided with a consumables unloading mechanism. Example

[0046] As attached Figure 9 To the attached Figure 11As shown, the present invention sets a detection position at the functional track 3 corresponding to the measurement area, and the detection position is composed of a cup position 16 to be detected, a detection cup position 17, and a detected cup position 18 arranged in sequence along the reaction cup conveying direction. A baffle lifting slot 15 that passes through the annular turntable body 1 is respectively opened on the functional track 3 corresponding to the cup position 16 to be detected, the detection cup position 17, and the detected cup position 18. The cup position 16 to be detected, the detection cup position 17, and the detected cup position 18 are respectively located between two adjacent baffle lifting slots 15; a photon counter fixing hole connected to the functional track 3 is opened on the annular disk body 1 corresponding to the detection cup position 17, and the photon counter 19 extends into the fixing hole to count the reaction cups passing through the detection cup position 17; as shown in the attached figure Figure 10 As shown, a baffle lifting mechanism is provided under the annular disk body 1, and the baffle lifting mechanism is provided with a light-blocking bottom plate 37. The upper surface of the light-blocking bottom plate 37 is respectively provided with detection baffles for separating the detection position and the cup position to be detected 16, the detection cup position 17, and the detected cup position 18. A linear lifting motor 40 is also provided for driving the light-blocking bottom plate to rise and fall; the detection baffles in the baffle lifting mechanism are respectively inserted into the two ends of the detection position and between the cup position to be detected 16, the detection cup position 17, and the detected cup position 18 along the baffle lifting slot 15, the light-blocking bottom plate 37 closes the bottom of the annular disk body 1, the annular cover plate 39 closes the upper part of the annular disk body 1, and the groove-type functional track 3 corresponding to the detection position of the annular disk body 1 forms a darkroom.

[0047] In this embodiment, the cup mouth of the reaction cup 61 is provided with an annular boss for supporting the reaction cup on the reaction cup slot hole of the conveying rack, and moves along the functional track, passing through the cup position 16 to be detected, the detection cup position 17, the detected cup position 18, and the waste liquid cup position 38 in sequence. The baffle lifting mechanism is provided with a linear lifting motor 40 fixed to the lower side of the fixed base plate 41 of the linear lifting motor, and an axial hole is provided on the fixed base plate 41 of the linear lifting motor. The output shaft of the linear lifting motor 40 extends through the axial hole and is connected to the light blocking base plate 37. A fixed side plate 42 connected to the annular disk is provided on the upper side of the fixed base plate 41 of the linear lifting motor, and a light blocking plate position baffle 43 for limiting the downward position of the light blocking base plate 37 and the detection baffle is provided on the fixed side plate 42. A position sensor 44 for detecting the position of the light blocking base plate and the detection baffle is further provided. The position sensor 44 can be provided above the light blocking plate position baffle 43 and can be connected and fixed by a position sensor fixing plate connected to the fixed side plate;

[0048] In order to reduce the impact when the detection baffle is plugged into the light-blocking bottom plate 37, a spring is provided between the top plate of the linear lift motor 40 and the light-blocking bottom plate. A shaft sleeve is provided at the bottom center of the light-blocking bottom plate 37, and a spring is provided in the shaft sleeve. The output shaft of the linear lift motor 40 extends into the shaft sleeve, and the top plate at the front end of the output shaft contacts the spring.

[0049] like Figure 10As shown, the detection baffles on the upper surface of the light-blocking bottom plate in the baffle lifting mechanism include a left end baffle 45 and a right end baffle 46 located at both ends for closing the two ends of the detection position, and also include a left detection cup position baffle 47 and a right detection cup position baffle 48 located between the left end baffle 45 and the right end baffle 46 for closing the two ends of the detection cup position. The heights of the four detection baffles are consistent and when the baffle lifting mechanism rises to the highest point, the detection baffles are respectively inserted into the two ends of the detection position and between the cup position to be detected, the detection cup position, and the detected cup position along the baffle lifting slot. The detection baffle is flush with the surface of the annular disk, forming a smooth working surface that facilitates the operation of the cuvette transport mechanism. To further improve the light-blocking effect on the detection cups, the left detection cup baffle 47 and the right detection cup baffle 48 have symmetrical L-shaped cross-sections. The bent portions of the left and right detection cup baffles are located near the photon counter end and fold outward away from the cuvettes. This allows the detection cups to be sealed by the upper cover, light-blocking bottom plate, and detection baffles, forming an independent small dark chamber inside, ensuring that photons cannot enter the dark chamber.

[0050] In the baffle lifting mechanism described in the present invention, a double annular boss 49 is provided around the four detection baffles on the upper surface of the light-blocking base plate to form a U-shaped cofferdam. At the same time, a double annular groove matching the double annular boss is provided at the corresponding position on the bottom surface of the annular turntable body to improve the fit between the light-blocking base plate and the bottom surface of the annular disk body, further improve the light-blocking effect, and prevent the excitation liquid from accidentally overflowing. Furthermore, a liquid storage tank 50 is provided between the left detection cup position baffle and the right detection cup position baffle on the upper surface of the light-blocking base plate to temporarily store the excitation liquid that is accidentally hit or the waste liquid that falls to avoid contamination of other parts of the equipment.

[0051] A lens glass fixing frame is set in the photon counter fixing hole opened on the side wall of the annular turntable of the present invention, and a photon counter lens glass is set. The photon counter 51 extends into the fixing hole and is fixed by the photon counter fixing plate. An O-ring is set between the photon counter 51 and the fixing hole, and the photon counter fixing plate 52 is connected and fixed to the fixed side plate.

[0052] The present invention provides a baffle lifting mechanism driven by a linear lifting motor. According to the collected data of the position sensor, the light shielding base plate and the detection baffle on the light shielding base plate are controlled to move up and down. After the reaction cup conveying mechanism sends the reaction cup to be tested into the detection cup position, the light shielding base plate and the detection baffle rise. The light shielding base plate and the detection baffle form a darkroom environment with the annular disk body and the upper cover. In addition, independent closed darkrooms are formed for the three reaction cups in the detection position. The reaction cups in the detection cup position are read using a photon counter. At this time, the two reaction cups adjacent to the reaction cup are isolated outside the independent darkroom. Therefore, the luminescent substances in the adjacent reaction cups will not interfere with the detection results. In addition, in order to achieve a good seal, the two components need to be absolutely tightly matched. In order to reduce the impact on the linear motor, the top plate of the linear lifting motor cooperates with the spring to contact the light shielding base plate to achieve flexible sealing of the light shielding base plate. The light shielding position baffle is provided to achieve upper and lower limit of the light shielding lifting mechanism. The lens glass is provided to effectively protect the photon counter. Example

[0053] The washing mechanism of the present invention adopts the following structure:

[0054] As attached Figure 13 As shown, the washing area is provided with a magnetic bead adsorption mechanism, in which a magnet mounting plate 53 is provided, and the magnet mounting plate 53 is mounted on the side of the functional track 3. More than two magnet mounting slots 54 are provided on the magnet mounting plate 53, and two magnets 55 are arranged side by side in each magnet mounting slot 54, and the magnets 55 face the reaction cup passing through the functional track 3; the magnetic pole directions of the two magnets 55 located in the same magnet mounting slot 54 are reversed, and the magnetic attraction directions of the two magnets at the same height in the adjacent magnet mounting slots 54 are also reversed; further, a pre-adsorption magnet mounting slot 56 is provided at the corresponding magnet mounting plate or the side wall of the functional track at the entrance of the washing area at the corresponding adsorption treatment starting position, and a magnet is installed in the pre-adsorption magnet mounting slot 56.

[0055] In the present invention, two pre-adsorption magnet mounting grooves 56 are arranged in parallel along the rotation direction of the annular disk 1 at the position corresponding to the starting position of the adsorption treatment on the magnet mounting plate 53. A magnet is arranged in each pre-adsorption magnet mounting groove, which is used to pre-adsorb the magnetic beads in the reaction cup before the washing process starts, thereby improving the adsorption and cleaning effect.

[0056] A lifting needle rack 57 is also provided on the annular cover plate 39 on the upper side of the disk body 1 in the washing area, and the magnetic bead adsorption mechanism is arranged on the side of the functional track 3, and the lifting bracket 57 is arranged above the functional track; the lifting needle rack 57 is provided with at least two groups of injection and aspiration combination needles 58, and the injection and aspiration combination needle 58 is composed of a aspiration needle 59 and an injection needle 60, wherein the needle tip of the lower end of the aspiration needle 59 is lower than the needle tip of the lower end of the injection needle 60, and the needle tip of the injection needle 60 is bent in the direction close to the aspiration needle 59, so as to ensure that when aspirating waste liquid, the needle tip of the aspiration needle can reach the bottom of the sample in the reaction cup and fully absorb impurities, and when injecting cleaning liquid, the needle tip of the injection needle is located above the needle tip of the aspiration needle, and the cleaning liquid injected by the injection needle can rinse the aspiration needle to avoid contamination of different samples.

[0057] Furthermore, the aspiration needle 59 and the injection needle 60 are both arranged above the reaction cup on the side away from the magnetic adsorption mechanism via the lifting needle frame 57, thereby ensuring that the aspiration needle descends to the opposite side of the reaction cup where the magnetic beads are adsorbed and aggregated during aspiration, thereby preventing the loss of magnetic beads.

[0058] The magnet mounting plate 53 is composed of a vertical plate and a bottom plate 62. The vertical plate 63 and the bottom plate 62 are vertically fixedly connected and have an L-shaped cross-section. The side of the vertical plate 63 is provided with more than two U-shaped grooves for placing magnets. The opening direction of the U-shaped groove is upward. The top of the vertical plate is provided with a pressure plate 64 for closing the openings of the two or more U-shaped grooves. The pressure plate is fixedly connected to the top of the vertical plate by screws, and the bottom plate is provided with a splicing tooth for engaging with the rotating chassis; two magnets are arranged side by side in the upper and lower U-shaped grooves on the side of the vertical plate, and a rust-proof iron plate is provided on the outside of the magnet to enhance the magnetism. Furthermore, the outside of the rust-proof iron plate is covered with a light-shielding tape to improve the light-shielding property of the entire mechanism; the vertical plate and the bottom plate are both arc-shaped to adapt to the inner side of the functional track on the disk body, and an installation notch that passes through the functional track for assembling the magnet mounting plate 53 is provided at the corresponding position on the washing area on the inner wall of the functional track of the disk body, and the installation notch is provided with a mounting groove that matches the splicing tooth 65.

[0059] During use, the reaction cup disc body 1 drives two or more reaction cups containing samples to be tested to advance in a set direction. The driving mechanism for driving the reaction cup rotating disk to rotate can drive the reaction cup rotating disk to advance one reaction cup position each time. When the washing process starts, the reaction cup first enters the pre-adsorption treatment area under the drive of the rotating disk. The single-grain magnet in the pre-adsorption magnet mounting groove on the magnet mounting plate pre-adsorbs the magnetic beads in the reaction cup, so that the magnetic beads originally scattered in the entire space of the reaction cup are close to the side where the magnetic bead adsorption mechanism is located. When the rotating disk drives the reaction cup into the double-grain magnet adsorption area, the magnetic beads are quickly adsorbed to the cup wall of the reaction cup close to the magnet. At this time, due to the use of double-grain magnet adsorption, the magnetic beads in the reaction cup are flattened on the cup wall of the reaction cup, and the agglomeration phenomenon is effectively reduced to avoid The needle tip of the injection needle is bent toward the direction close to the pipette needle, thereby ensuring that when absorbing waste liquid, the needle tip of the pipette needle can reach the bottom of the sample in the reaction cup and fully absorb impurities; when injecting cleaning liquid, the needle tip of the injection needle is located above the needle tip of the pipette needle, and the cleaning liquid injected by the injection needle can rinse the pipette needle to avoid contamination of different samples; in addition, the pipette needle and the injection needle are both arranged on the side of the reaction cup deviating from the magnet adsorption mechanism above the reaction cup via the lifting bracket, thereby ensuring that the pipette needle descends to the opposite side of the reaction cup where the magnetic beads are adsorbed and agglomerated when absorbing liquid, thereby avoiding loss of magnetic beads.

[0060] Compared with the prior art, the present invention can effectively improve the cleaning effect and reduce the loss of magnetic beads without increasing the number of cleaning times, and has significant advantages such as reasonable structure, simple operation, and reliable work. Example

[0061] The mixing mechanism of the present invention adopts the following structure:

[0062] As attached Figure 15 As shown, a mixing mechanism is fixed on the functional track 3 corresponding to the mixing zone, so that the mixing sleeve in the mixing mechanism is located below the reaction cup in the functional track 3 of the mixing zone; the mixing sleeve 69 is sleeved on the outside of the rotating shaft 70, and a latch 71 is provided on the rotating shaft 70. A spiral groove hole 72 arranged around the cylinder body is provided on the cylinder body of the mixing sleeve 69, and both ends of the latch 71 are located in the spiral groove hole 72; the upper port of the mixing sleeve 69 is used to accommodate the reaction cup 61, and the inner diameter circle and the outer diameter circle of the upper port of the mixing sleeve are eccentrically arranged to have an eccentric hole 73; the rotating shaft is connected to the output shaft of the motor, and the motor 74 drives the rotating shaft to drive the latch to rotate.

[0063] In order to obtain an adjustable moment of inertia, the mixing sleeve 69 adopts a double-layer sleeve structure. The double-layer sleeve is made of different materials, wherein the inner sleeve 75 is made of PPS plastic and the outer sleeve 76 is made of a metal sleeve. The present invention uses an aluminum outer sleeve, and the PBC inner sleeve is interference fit with the aluminum outer sleeve. Then, a spiral groove hole is opened on the double-layer sleeve to pass through the inner and outer sleeves at the same time. The length of the spiral groove hole 72 is greater than the circumference of the outer diameter of the sleeve; the double-layer sleeve structure can ensure that the moment of inertia of the entire mixing sleeve is adjustable, and avoid the moment of inertia being too large or too small.

[0064] The spiral groove hole 72 described in the present invention is located in the middle and lower part of the mixing sleeve 69, so that when the rotating shaft rotates, the pin drives the mixing sleeve to move, and drives the mixing sleeve to rise along the rotating shaft through rotational inertia. At this time, the pin on the rotating shaft gradually rotates from the high position of the spiral groove hole to the low position, the upper port of the mixing sleeve rises and accommodates the lower end of the current reaction cup in the upper port, the motor 74 continues to rotate forward, the rotating shaft continues to rotate, the mixing sleeve is driven to rotate and vibrate the reaction cup located at the upper port of the mixing sleeve 69, completing the shaking and mixing; when the motor 74 is reversed, the rotating shaft rotates in the opposite direction, the pin rotates from the low position of the spiral groove hole to the high position, driving the mixing sleeve to fall.

[0065] The present invention is provided with a bracket, which includes a base 77 and a mixing sleeve bracket 78. The mixing sleeve bracket 78 is connected to the base via a vertical plate 79. An axial hole is provided on the base 77. The motor 74 is located below the base. The output shaft of the motor extends through the axial hole and is connected to the rotating shaft above the base. The mixing sleeve bracket 78 is located above the base and is provided with a through hole for accommodating the mixing sleeve. There is a gap between the through hole wall and the outer wall of the mixing sleeve. Since the sample to be processed is liquid, there is a risk of spillage. Therefore, a guide groove 80 is further provided on the upper surface of the mixing sleeve bracket to guide the spilled liquid to prevent the liquid from entering the interior of the device.

[0066] The present invention is also provided with an in-position detection circuit, which is used to detect whether the mixing sleeve 2 falls back to the base. Since the device is used in a continuous detection device, after the mixing is completed, the mixing sleeve should fall back to the bottom of the rotating shaft on the base. At this time, the reaction cup is separated from the upper port of the mixing sleeve and then enters the next level process through the conveying mechanism. At this time, if the mixing sleeve cannot fall back in time, it will cause a cup jam problem, which will not only interrupt the entire detection process, but also damage the sample to be tested and the equipment. In order to avoid this problem, the present invention provides a position sensor 81 fixed on the base in the in-position detection circuit. The position sensor detects whether the mixing sleeve has fallen back to the lowest position. If it has fallen back, the motor reversal is not started. Otherwise, the motor reversal is controlled by the motor control circuit, so that the rotating shaft drives the latch to lower the mixing sleeve to the base.

[0067] A support ring 82 is provided on the outside of the mixing sleeve described in the present invention. The support ring 82 can be realized by a boss or an annular plate arranged around the outer wall of the mixing sleeve. The support ring is located on the upper part of the mixing sleeve and has an outer diameter larger than the outer diameter of the through hole on the mixing sleeve bracket. It is used to support the mixing sleeve on the through hole of the mixing sleeve bracket in a static state, and can generate interaction force with the upper wall of the through hole on the mixing sleeve bracket during the rotation start-up process, so as to promote the relative rotation of the mixing sleeve and then rotate and rise; preferably, an annular plate is used, which can not only play a supporting role, but also prevent the liquid sample in the reaction cup placed in the mixing sleeve from spilling into the interior of the sleeve, thereby avoiding corrosion of the equipment.

[0068] The latch on the rotating shaft of the present invention is made of POM plastic with self-lubricating properties and is in a strip shape. A transverse latch hole is provided on the rotating shaft and passes through the rotating shaft. The latch is inserted into the latch hole. Since the device rotates at high speed with the motor during operation, in order to prevent the latch from being thrown out, a fixing hole is further provided on the rotating shaft along the length direction of the rotating shaft. The fixing hole is connected to the transverse latch slot hole. The clamping piece is pressed into the fixing hole and abuts against the latch, and the upper end of the fixing hole is then locked with a screw.

[0069] The present invention further provides a water guide hole 83 on the upper side wall of the mixing sleeve for discharging overflow liquid.

[0070] In order to prevent the reaction cup in the mixing sleeve from being thrown out, a top plate can be provided on the upper part of the mixing sleeve.

[0071] The present invention is aimed at mixing samples for chemiluminescent immunoassay. Since the effective ingredients in such samples are carried by magnetic beads, in order to prevent large-scale aggregation of magnetic beads, two magnets can be arranged opposite to each other on the upper surface of the mixing sleeve bracket, wherein the magnetic poles of the two magnets 84 near one end of the mixing sleeve are opposite, so that the reaction cup repeatedly passes through the magnetic field during the mixing process. Under the action of the magnetic force of the magnetic field, the magnetic beads in the reaction cup form relative motion with the reaction cup, thereby improving the mixing effect.

[0072] Compared with the prior art, the present invention provides a shaking sleeve with adjustable moment of inertia, which can flexibly select the rotational speed of the shaking sleeve after it is raised, and can drive the shaking sleeve to rise quickly under low acceleration; and can flexibly adjust the mixing effect without changing the motor drive; and is compatible with multiple motors, has a good mixing effect, and basically eliminates bubbles. Example

[0073] The consumables unloading mechanism of the present invention adopts the following structure:

[0074] As attached Figure 12As shown, the cup body of the reaction cup in the present invention is in the shape of a flat-mouthed long strip. Furthermore, the cross-section of the cup body of the reaction cup is rectangular. During the transmission process, the main part of the reaction cup body is placed on the functional track, and the cup wall of the reaction cup body with a larger area faces the side where the photon counter is provided. The transverse width of the reaction cup slot 9 on the functional transmission rack 8 is greater than the length of the reaction cup mouth, and the width in the movement direction is less than the length of the reaction cup mouth. The purpose of this arrangement is to facilitate the unloading of discarded reaction cups. An unloading mechanism is provided on the unloading area of ​​the functional track 3. The reaction cup unloading mechanism includes an unloading motor 53 and an unloading paddle 54. The motor shaft hole is provided on the cover above the unloading area disk body. The unloading motor 53 is fixed above the top cover of the unloading area and moves along the motor. The machine shaft hole is connected to the unloading paddle 54 located below the unloading top cover; the unloading paddle 54 is fixedly connected to the motor via a cotter pin, and the unloading paddle 54 can be provided with a first toggle boss 55 and a second toggle boss 56 respectively located on both sides of the reaction cup. During unloading, the motor drives the unloading paddle to rotate 90°, and the first toggle boss 55 and the second toggle boss 56 simultaneously toggle the upper port of the reaction cup 57 to rotate; further, the unloading motor is fixed to the unloading top cover via a motor seat, and further, an unloading sensor can be provided on the unloading top cover to detect whether there is a reaction cup to be unloaded at the current unloading position through the unloading sensor. If so, the unloading motor is controlled by the motor control circuit to perform a rotation action, otherwise unloading is not performed, thereby improving the overall processing efficiency.

[0075] A discharge channel 58 for introducing the cuvette dropped from the cuvette slot 9 into a waste bucket is provided below the functional track 3 corresponding to the discharge position, and a waste bucket is provided below the discharge channel. Example

[0076] The washing mechanism of the present invention adopts a double-sided washing method and adopts the following structure:

[0077] As attached Figure 14 As shown, the double-sided washing mechanism is provided with a first magnetic bead adsorption mechanism and a second magnetic bead adsorption mechanism separately located on both sides of the functional track 3 of the washing area, wherein the first magnetic bead adsorption mechanism and the second magnetic bead adsorption mechanism are both provided with magnets, and the magnets in the first magnetic bead adsorption mechanism and the second magnetic adsorption mechanism are asymmetrically arranged relative to the functional track.

[0078] The first magnetic bead adsorption mechanism / the second magnetic bead adsorption mechanism are both provided with a magnet mounting plate 53 for fixing magnets, and the magnet mounting plate 53 is provided with a magnet mounting groove on the side facing the functional track 3. Preferably, more than two magnet mounting grooves 54 are provided for respectively installing more than two groups of magnets, so as to complete multiple magnetic adsorptions in the process of the sample passing through the functional track of the washing area. The magnet mounting grooves on the magnet mounting plates in the first magnetic bead adsorption mechanism and the second magnetic bead adsorption mechanism are asymmetrically provided relative to the functional track, so that the sample is staggeredly adsorbed by the magnetic adsorption mechanisms on both sides, ensuring that the magnetic beads are fully dispersed after the previous adsorption before the next adsorption, thereby avoiding stratification and wrapping of impurities by the magnetic beads.

[0079] Multiple sample cuvettes 61 containing samples to be processed continuously pass through the functional track 3. A liquid aspiration / dispensing needle assembly is also provided above the functional track 3. In the figure, two or more cuvettes 61 continuously move from right to left, first passing through the pre-adsorption zone 3. The pre-adsorption positions in the pre-adsorption zone 3 can be equipped with a single magnet. Two groups of pre-adsorption positions are provided in the pre-adsorption zone 3, each group corresponding to a unit displacement distance of the cuvette 2 (the distance the cuvette moves forward when driven by the conveyor rack).

[0080] After the pre-adsorption area 66, an adsorption area 67 is set. The magnet mounting plate 53 in the adsorption area 67 is filled with the adsorption position of the double magnet. The adsorption position is also set in two or more consecutive positions. Then, a non-filled area 68 is set on the magnet mounting plate 53 on the same side as the aforementioned adsorption position, and a filled area is set on the magnet mounting plate on the other side (the adsorption area and the magnet mounting plate 53 on the other side corresponding to the pre-adsorption area are both non-filled areas). At this time, Figure 14 The full filling method is not used in the embodiment, but a non-full filling method can be further selected. That is, in this case, the magnet mounting positions of the two or more magnet mounting positions on the magnet mounting plate 53 on the other side, which are closer to the direction of entry of the reaction cup, can be in a non-filled state. Practical application has shown that this structure can further improve the dispersion effect of the enclosed impurities.

[0081] In the figure, the magnet mounting plate on one side of the pre-adsorption area 66 is provided with an adsorption area 67 on the other side of the non-filling area 68, and more than two groups of double magnet filling positions are provided in the adsorption area 67.

[0082] Through this multiple alternating adsorption, impurities contained in the sample are ensured to be fully removed. In addition, during the alternating adsorption process, each change in direction of magnetic adsorption ensures that there are more than two consecutive magnet installation positions (whether in the filling area or the non-filling area). This structure can effectively avoid magnetic conflicts between adjacent magnets and ensure the reliability of magnetic bead adsorption in adjacent areas.

[0083] The structure of the magnet mounting plate is similar to that of Example 2, with two or more magnet mounting slots provided thereon. Two magnets are arranged side by side in each magnet mounting slot 11. The magnetic poles of the two magnets in the same magnet mounting slot are reversed, and the magnetic attraction directions of the two magnets at the same height in adjacent magnet mounting slots are also reversed. Due to the use of dual-particle magnet adsorption, the magnetic beads in the reaction cup are spread flat on the wall of the reaction cup, effectively reducing agglomeration and preventing impurities from being wrapped by multiple layers of agglomerated magnetic beads and affecting the cleaning effect.

[0084] The washing mechanism of this embodiment is particularly suitable for a reaction rotating disk chemiluminescence immunoassay, in which a functional track for sample passage is provided on the reaction rotating disk; two pre-adsorption magnet mounting grooves are arranged in parallel along the rotation direction of the reaction cup rotating disk at a position corresponding to the starting position of the adsorption treatment on the magnet mounting plate, and a magnet is arranged in each pre-adsorption magnet mounting groove for pre-adsorption treatment of the magnetic beads in the reaction cup before the start of the washing process, thereby improving the adsorption and cleaning effect; compared with the existing technology, the present invention can perform multiple adsorption on both sides of the sample to be processed passing through the functional track, thereby effectively solving the problem of impurities that cannot be removed due to the inability to fully disperse impurities wrapped between the magnetic beads during the existing unilateral adsorption, and has significant advantages such as reasonable structure and simple operation.

Claims

1. A chemiluminescent immunoassay analyzer with multiple reaction modes, characterized in that: A chemiluminescent immunoassay reaction disk with multiple reaction modes is provided, which is provided with an annular disk body, and an annular track groove is provided on the disk body, characterized in that an annular incubation track and an annular functional track are respectively provided on the disk body, and functional positions for adding samples or adding reagents or mixing or washing or measuring or unloading are respectively provided on the annular functional track, and at least one lane changing position is also provided on the annular functional track, and a gripper mechanism for realizing the current reaction cup switching functional track or incubation track is provided on the lane changing position, wherein a loading area, a first sample adding area, a first mixing area, a first lane changing gripper area, a first washing area, a second sample adding area, a second mixing area, a second lane changing gripper area, a second washing area, a third mixing area, a measurement area, and an unloading area are sequentially provided on the chemiluminescent immunoassay reaction disk with multiple reaction modes, and a detection position is provided at the functional track corresponding to the measurement area, and the detection position is composed of a cup position to be detected, a detection cup position, and a detected cup position sequentially arranged along the reaction cup conveying direction. Baffle lifting slots that pass through the annular turntable body are respectively provided on the functional tracks of the measuring cup position and the tested cup position, and the cup position to be tested, the testing cup position and the tested cup position are respectively located between two adjacent baffle lifting slots; a photon counter fixing hole connected to the functional track is provided on the annular disk body corresponding to the testing cup position, and the photon counter extends into the fixing hole to count the reaction cups passing through the testing cup position; a baffle lifting mechanism is provided under the annular disk body, and the baffle lifting mechanism is provided with a light-blocking bottom plate, and the upper surface of the light-blocking bottom plate is respectively provided with a detection baffle for separating the testing position and the cup position to be tested, the testing cup position and the tested cup position, and a linear lifting motor is also provided for driving the light-blocking bottom plate to rise and fall; the detection baffle in the baffle lifting mechanism is respectively inserted into the two ends of the testing position and between the cup position to be tested, the testing cup position and the tested cup position along the baffle lifting slots, the light-blocking bottom plate closes the bottom of the annular disk body, the annular cover plate closes the upper part of the annular disk body, and the groove-type functional track part corresponding to the testing position of the annular disk body forms a darkroom.

2. The chemiluminescence immunoassay analyzer with multiple reaction modes according to claim 1, characterized in that: The washing area is provided with a washing mechanism, the mixing area is provided with a mixing mechanism, and the unloading area is provided with a consumables unloading mechanism.

3. The chemiluminescence immunoassay analyzer with multiple reaction modes according to claim 1, characterized in that: The measuring area is provided with a measuring mechanism; the washing area is provided with a washing mechanism, the mixing area is provided with a mixing mechanism, and the unloading area is provided with a consumables unloading mechanism. The annular incubation track and the annular functional track are concentrically arranged, and the annular functional track is located outside the annular incubation track.

4. The chemiluminescence immunoassay analyzer with multiple reaction modes according to claim 1, characterized in that: A heating layer is provided at the lower part of the annular incubation track, and the heating layer consists of 7 heating zones.

5. The chemiluminescence immunoassay analyzer with multiple reaction modes according to claim 1, characterized in that: The annular incubation track is provided with an incubation transmission rack, and the annular functional track is provided with a functional transmission rack. Both the incubation transmission rack and the functional transmission rack are annular and have two or more reaction cup slots distributed at equal intervals, wherein the reaction cup slots are located above the track groove. The incubation transmission rack and the functional transmission rack are driven by an electric motor through a gear transmission mechanism to rotate around the center of the turntable, driving the reaction cups to move along the incubation track and the functional track respectively.

6. The chemiluminescence immunoassay analyzer with multiple reaction modes according to claim 5, characterized in that: The function transmission rack operates in the following structure: a continuous sawtooth meshing with the function motor gear is provided on the side of the function transmission rack, and the output shaft of the function motor drives the function motor gear to rotate, thereby continuously driving the function transmission rack to operate.

7. The chemiluminescence immunoassay analyzer with multiple reaction modes according to claim 5, characterized in that: The incubation transmission rack is driven to rotate by a separate incubation transmission motor, or continuous saw teeth are provided on the outer ring of the incubation transmission rack, and the rack is driven to rotate by a functional motor through a gear transmission mechanism.

8. The chemiluminescence immunoassay analyzer with multiple reaction modes according to claim 1, characterized in that: Two sets of lane-changing gripper mechanisms are provided on the annular turntable, which are used to grab the reaction cups on the incubation track to the functional track, and to grab the reaction cups on the functional track to the incubation track.

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