A biosafety monkey negative pressure isolation device

Abstract

The invention discloses a biosafety monkey negative pressure isolation device, comprising an isolation box, an air filtration unit, a power ventilation unit and a control unit. The isolation box is provided with a box door, the box door is hingedly connected to the isolation box, a plurality of isolation cages are provided in the isolation box, a smooth door is provided on the side of the box door close to the isolation cage, the smooth door is slidably connected to the isolation box, a feces tray is provided at the bottom of the cage, the feces tray is connected to the inner cavity of the isolation cage, and a sealing bag is connected to the bottom of the smooth door. The biosafety monkey negative pressure isolation device of the present invention puts the isolation box in a negative pressure state, and when the box door and the smooth door are opened, the directional flow of air can be effectively guaranteed to avoid leakage of harmful gases in the isolation box; at the same time, when transferring feces of experimental animals, the feces tray directly enters the sealing bag, and the sealing bag is sealed and disinfected, effectively preventing high-concentration harmful biological aerosols in the feces tray from leaking to the environment outside the isolator.

Description

Technical Field

[0001] The invention relates to the technical field of biosafety protection equipment and peripheral supporting facilities thereof, and in particular to a biosafety monkey negative pressure isolation device. Background Art

[0002] In recent years, new and emerging epidemics have continued to break out around the world, posing serious threats to human health and social stability. Laboratory animals are indispensable experimental materials in life science research and play an important role in the human fight against pathogenic microorganisms, especially non-human primates.

[0003] Due to the characteristics of non-human primates, it is difficult to raise them in a completely closed isolator. At the same time, since non-human primates are active, smart and aggressive, the risk of infection and leakage is higher when conducting non-human primate infection experiments. Once improperly controlled or operated, it will cause serious safety risks and seriously threaten the safety of laboratory personnel and the surrounding environment. For this reason, it is urgent to develop a monkey negative pressure isolator that is easy to operate and has high biosafety.

[0004] The monkey isolators currently produced and used in my country are mainly used for the breeding of non-infected animals. Some products have been appropriately modified to meet the needs of pathogenic microorganism operations, but they still cannot fully meet the biosafety requirements for the infection and breeding of highly pathogenic microorganisms in non-human primate experimental animals. Since non-human primates are mainly raised in semi-open isolation cages, this requires that under normal operation, the isolator needs to effectively control the exposure of pathogenic microorganisms to the external environment through airflow control, physical isolation and other means.

[0005] Although the monkey negative pressure isolator developed abroad can meet the requirements of biosafety to a certain extent, it is difficult to control the exposure to harmful aerosols during some operations. For example, during the transfer of feces, it is difficult to effectively prevent the exposure of high concentrations of pathogenic microorganisms in the feces to the environment.

[0006] Therefore, how to change the current situation in the prior art where the isolation device cannot control the exposure of pathogenic microorganisms in the environment has become an urgent problem to be solved by those skilled in the art. Summary of the invention

[0007] The purpose of the present invention is to provide a biosafety monkey negative pressure isolation device to solve the problems existing in the above-mentioned prior art, effectively control the exposure of pathogenic microorganisms in the environment, and improve the biosafety of the isolation device.

[0008] To achieve the above-mentioned purpose, the present invention provides the following scheme: The present invention provides a biosafety monkey negative pressure isolation device, comprising an isolation box, an air filtration unit, a power ventilation unit and a control unit, the isolation box is provided with a box door, the box door is hingedly connected to the isolation box, a plurality of isolation cages are provided in the isolation box, the number of the isolation cages is consistent with the number of the box doors and corresponds one to one, the inner cavity of the isolation cage is connected to the inner cavity of the isolation box, the isolation cage can accommodate experimental animals, a smooth door is provided on the side of the box door close to the isolation cage, the smooth door is located between the box door and the isolation cage, the smooth door is slidably connected to the isolation box, and the bottom of the cage A feces tray is provided, and the feces tray is communicated with the inner cavity of the isolation cage. A sealing bag is connected to the bottom of the smooth door, and the sealing bag is detachably connected to the smooth door. When the sealing bag is connected to the smooth door, a sealing element is provided at the connection between the sealing bag and the smooth door. When the smooth door is closed, the sealing bag is arranged opposite to the feces tray, and the feces tray can enter the sealing bag. The isolation box is connected to the external environment through the power ventilation unit, and the air filter unit is arranged between the power ventilation unit and the isolation box. The air filter unit can efficiently filter the airflow, and the air filter unit and the power ventilation unit are both connected to the control unit.

[0009] Preferably, the isolation cage has a fixed baffle and a mesh baffle on one side close to the smooth door, the mesh baffle is arranged on the top of the fixed baffle, an animal entrance and exit are opened on the fixed baffle, and the feces tray is arranged at the bottom of the animal entrance and exit; the box door and the smooth door are both made of transparent material, the smooth door includes a first sliding door and a second sliding door arranged in parallel, the first sliding door can block the animal entrance and exit, the distance between the first sliding door and the isolation cage is larger than the distance between the second sliding door and the isolation cage, and the sealing bag is detachably connected to the first sliding door.

[0010] Preferably, a connecting flange is provided at the bottom of the first sliding door, and the connecting flange can accommodate the passage of the feces tray. The sealing bag can be mounted on the outside of the connecting flange, and the outer side wall of the connecting flange has a sealing groove. When the sealing bag is mounted on the outside of the connecting flange, a sealing ring is mounted on the outside of the sealing bag, and the sealing ring is located in the sealing groove. The sealing bag is made of a flexible material.

[0011] Preferably, an internal baffle and a pull rod are provided in the isolation cage, the internal baffle is slidably connected to the isolation cage, the internal baffle is provided in parallel with the fixed baffle, the internal baffle is connected to the pull rod, the pull rod is slidably connected to the fixed baffle, the pull rod has a plurality of slots, and the pull rod can be connected to the fixed baffle by means of the slots; a waterer is provided on the grid baffle, and a feeder is provided on the fixed baffle.

[0012] Preferably, the powered ventilation unit comprises an air supply system and an exhaust system, the air supply system comprises an air supply fan and an air supply duct, the air supply fan is connected to the air supply duct, the air supply duct is arranged between the box door and the smooth door, the air supply duct is provided with air supply jet holes, the smooth door is provided with air inlet holes, the exhaust system comprises an exhaust fan and an exhaust duct, the exhaust fan is connected to the exhaust duct, the exhaust fan is connected to the inner cavity of the isolation box body, the exhaust duct is connected to the external environment, and exhaust holes are arranged on the side wall of the isolation cage opposite to the smooth door.

[0013] Preferably, there are two groups of isolation cages, the air supply duct is arranged between the two groups of isolation cages, the two groups of isolation cages are symmetrically arranged with the axis of the air supply duct as the center line, a plurality of air supply jet holes are symmetrically arranged toward the isolation cages, the air inlet hole is arranged at the end of the smooth door away from the air supply duct, each group of isolation cages is stacked and arranged from top to bottom, and the diameter of the air supply jet hole gradually increases along the air supply direction of the air supply duct; a plurality of the exhaust holes are arranged in a rectangular array, the air inlet holes are in two rows, and the two rows of air inlet holes are staggered.

[0014] Preferably, the air inlet fan and the exhaust fan are both arranged at the top of the isolation box body, the air filtration unit includes an air supply high-efficiency filter, a heating module, an exhaust pre-filter, a static pressure box and an exhaust high-efficiency filter, the air supply high-efficiency filter and the heating module are both connected to the air supply fan, the static pressure box and the exhaust pre-filter are arranged at the exhaust hole, and the exhaust high-efficiency filter is arranged between the exhaust pre-filter and the exhaust fan.

[0015] Preferably, the air supply high efficiency filter and the exhaust high efficiency filter are both provided with a gas disinfection interface, and the gas disinfection interface can be connected to a disinfection machine, and a closed valve and a closed cover are provided at the gas disinfection interface; the air outlets of the air supply high efficiency filter and the exhaust high efficiency filter are both provided with aerosol sampling ports.

[0016] Preferably, a sensor is provided at the box door, and the sensor can detect the opening and closing status of the box door. The sensor, the air supply fan, and the exhaust fan are all connected to the control unit.

[0017] Compared with the prior art, the present invention has achieved the following technical effects: the biosafety monkey negative pressure isolation device of the present invention comprises an isolation box, an air filtration unit, a power ventilation unit and a control unit; the isolation box is provided with a box door, the box door is hingedly connected to the isolation box, a plurality of isolation cages are provided in the isolation box, the number of isolation cages is consistent with the number of box doors and corresponds one to one, the inner cavity of the isolation cage is connected to the inner cavity of the isolation box, the isolation cage can accommodate experimental animals, a smooth door is provided on the side of the box door close to the isolation cage, the smooth door is located between the box door and the isolation cage, and the smooth door is slidably connected to the isolation box A feces tray is arranged at the bottom of the cage, and the feces tray is connected to the inner cavity of the isolation cage. A sealing bag is connected to the bottom of the smooth door, and the sealing bag and the smooth door are detachably connected. When the sealing bag is connected to the smooth door, a sealing element is arranged at the connection between the sealing bag and the smooth door. When the smooth door is closed, the sealing bag is arranged opposite to the feces tray, and the feces tray can enter the sealing bag; the isolation box is connected to the external environment through the power ventilation unit, and the air filter unit is arranged between the power ventilation unit and the isolation box. The air filter unit can efficiently filter the airflow, and the air filter unit and the power ventilation unit are both connected to the control unit. When the biosafety monkey negative pressure isolation device of the present invention is working, the isolation box is in a negative pressure state through the control unit and the power ventilation unit. When the box door and the smooth door are opened, the directional flow of air can be effectively guaranteed to prevent the leakage of harmful gases in the isolation box; at the same time, a feces tray is arranged at the bottom of the isolation cage, and a sealing bag is arranged outside the feces tray. When the feces tray needs to be taken out, the feces tray directly enters the sealing bag, and the sealing bag is sealed and then disinfected and sterilized, effectively preventing the high concentration of harmful biological aerosols in the feces tray from leaking to the environment outside the isolator; in addition, the power ventilation unit is connected to an air filtration unit, which can efficiently filter the gas entering and discharged from the isolation box, achieve the purpose of controlling the quality of air intake and exhaust, improve the operability of the isolation device, avoid the emission of harmful air to the outside of the isolation box to cause environmental pollution, and improve the operating safety of the experimenter. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0019] Figure 1 It is a structural schematic diagram of the biosafety monkey negative pressure isolation device of the present invention;

[0020] Figure 2It is a structural schematic diagram of another embodiment of the biosafety monkey negative pressure isolation device of the present invention;

[0021] Figure 3 It is a schematic diagram of the top view of the biosafety monkey negative pressure isolation device of the present invention;

[0022] Figure 4 It is a partial structural enlarged schematic diagram of the biosafety monkey negative pressure isolation device of the present invention;

[0023] Figure 5 It is a structural schematic diagram of an isolation cage of a biosafety monkey negative pressure isolation device of the present invention;

[0024] Figure 6 It is a front view structural schematic diagram of an isolation cage of a biosafety monkey negative pressure isolation device of the present invention;

[0025] Figure 7 It is a schematic cross-sectional structure diagram of an isolation cage of a biosafety monkey negative pressure isolation device of the present invention;

[0026] Among them, 1 is an isolation box body, 2 is a box door, 3 is an isolation cage, 301 is a movable baffle, 302 is a pull rod, 4 is a smooth door, 401 is a first sliding door, 402 is a second sliding door, 5 is a sealing bag, 6 is a connecting flange, 7 is an air supply fan, 8 is an air supply duct, 9 is an air inlet, 10 is an exhaust fan, 11 is an exhaust duct, 12 is an exhaust hole, 13 is an air supply high efficiency filter, 14 is an exhaust high efficiency filter, and 15 is a sealing ring. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] The purpose of the present invention is to provide a biosafety monkey negative pressure isolation device to solve the problems existing in the above-mentioned prior art, effectively control the exposure of pathogenic microorganisms in the environment, and improve the biosafety of the isolation device.

[0029] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0030] Please refer to Figure 1-7 ,in, Figure 1 It is a structural schematic diagram of the biosafety monkey negative pressure isolation device of the present invention, Figure 2FIG. 1 is a schematic structural diagram of another embodiment of the biosafety monkey negative pressure isolation device of the present invention. Figure 3 It is a top view of the structure of the biosafety monkey negative pressure isolation device of the present invention, Figure 4 It is a partial structural enlarged schematic diagram of the biosafety monkey negative pressure isolation device of the present invention, Figure 5 This is a schematic structural diagram of an isolation cage of a biosafety monkey negative pressure isolation device of the present invention. Figure 6 This is a front view structural schematic diagram of an isolation cage of a biosafety monkey negative pressure isolation device of the present invention, Figure 7 The present invention is a schematic diagram of the cross-section structure of an isolation cage of a biosafety monkey negative pressure isolation device.

[0031] The invention provides a biosafety monkey negative pressure isolation device, comprising an isolation box 1, an air filtering unit, a power ventilation unit and a control unit. The isolation box 1 is provided with a box door 2, which is hingedly connected to the isolation box 1. A plurality of isolation cages 3 are arranged in the isolation box 1, and the number of the isolation cages 3 is consistent with the number of the box doors 2 and corresponds one to one. The inner cavity of the isolation cage 3 is connected to the inner cavity of the isolation box 1, and the isolation cage 3 can accommodate experimental animals. A smooth door 4 is arranged on one side of the box door 2 close to the isolation cage 3, and the smooth door 4 is located between the box door 2 and the isolation cage 3. The smooth door 4 is slidably connected to the isolation box 1, and the bottom of the cage A feces tray is provided, and the feces tray is communicated with the inner cavity of the isolation cage 3. A sealing bag 5 is connected to the bottom of the smooth door 4, and the sealing bag 5 and the smooth door 4 are detachably connected. When the sealing bag 5 is connected to the smooth door 4, a sealing element is provided at the connection between the sealing bag 5 and the smooth door 4. When the smooth door 4 is closed, the sealing bag 5 is arranged opposite to the feces tray, and the feces tray can enter the sealing bag 5; the isolation box 1 is connected to the external environment through a power ventilation unit, and an air filter unit is arranged between the power ventilation unit and the isolation box 1. The air filter unit can efficiently filter the airflow, and the air filter unit and the power ventilation unit are both connected to the control unit.

[0032] The biosafety monkey negative pressure isolation device of the present invention has an isolation cage 3 in the isolation box, and the isolation cage 3 is provided with a separate smooth door 4. The box door 2 and the smooth door 4 are provided at the same time, so that the isolation device has good mechanical isolation. Several isolation cages 3 can be used to feed and manage experimental animals separately. When the isolation device is working, the isolation box 1 is in a negative pressure state through the control unit and the power ventilation unit. When the box door 2 and the smooth door 4 are opened, the directional flow of air can be effectively guaranteed to prevent the leakage of harmful gases in the isolation box 1; at the same time, a feces tray is arranged at the bottom of the isolation cage 3, and a sealing bag 5 is arranged outside the feces tray. When the feces tray needs to be taken out, the feces tray is directly placed in the sealing bag 5, and the sealing bag 5 is sealed and then disinfected and sterilized, so as to effectively prevent the high concentration of harmful biological aerosols in the feces tray from leaking to the environment outside the isolator; in addition, the power ventilation unit is connected to an air filtration unit, which can filter the gas entering and discharged from the isolation box 1, so as to achieve the purpose of controlling the quality of air intake and exhaust, improve the operability of the isolation device, and prevent harmful air from being discharged to the outside of the isolation box 1 to cause environmental pollution, thereby improving the operating safety of the experimenter.

[0033] Among them, the isolation cage 3 has a fixed baffle and a mesh baffle on the side close to the smooth door 4. The mesh baffle is arranged on the top of the fixed baffle. An animal entrance and exit are opened on the fixed baffle. The feces tray is arranged at the bottom of the animal entrance and exit to facilitate the collection of feces of experimental animals. The box door 2 and the smooth door 4 are both made of transparent material, which is convenient for observing experimental animals. The smooth door 4 includes a first sliding door 401 and a second sliding door 402 arranged in parallel. The first sliding door 401 can block the animal entrance and exit. The distance between the first sliding door 401 and the isolation cage 3 is larger than the distance between the second sliding door 402 and the isolation cage 3, that is, the first sliding door 401 is on the side close to the box door 2, which is convenient for connecting the sealing bag 5, and the sealing bag 5 and the first sliding door 401 are detachably connected.

[0034] In this specific embodiment, a connecting flange 6 is set at the bottom of the first sliding door 401, and the connecting flange 6 can accommodate the passage of a feces tray. The sealing bag 5 can be mounted on the outside of the connecting flange 6. The outer side wall of the connecting flange 6 has a sealing groove. When the sealing bag 5 is mounted on the outside of the connecting flange 6, a sealing ring 15 is mounted on the outside of the sealing bag 5. The sealing ring 15 is located in the sealing groove. The sealing ring 15 presses the sealing bag 5 against the connecting flange 6 to prevent harmful gases from leaking out through the gap between the sealing bag 5 and the connecting flange 6. The sealing bag 5 is made of a flexible material and can be conveniently folded and placed between the box door 2 and the first sliding door 401. The sealing bag 5 can be made of plastic material and can be folded and sealed. When transferring experimental animal feces, the feces tray is pulled into the sealing bag 5, sealed with a heat sealer, and placed in an autoclave together with the feces tray for disinfection and sterilization.

[0035] In order to facilitate operations such as injection and blood drawing on experimental animals, an internal baffle 301 and a pull rod 302 are set in the isolation cage 3. The internal baffle 301 is slidably connected to the isolation cage 3. The internal baffle 301 is set parallel to the fixed baffle. The experimental animal is located between the fixed baffle and the internal baffle 301. The internal baffle 301 is connected to the pull rod 302. The pull rod 302 is slidably connected to the fixed baffle. The pull rod 302 has multiple slots. The pull rod 302 can be connected to the fixed baffle by using the slots. The relative position between the two can be adjusted by using different slots on the pull rod 302 to connect with the fixed baffle, while improving the stability of the internal baffle 301. When it is necessary to operate the experimental animals, pull the pull rod 302, and the internal baffle 301 slides toward the fixed baffle, compressing the activity space of the experimental animals, forcing the experimental animals to move toward the fixed baffle and close to the operator. The experimental tools can pass through the mesh baffle to complete the injection, blood collection and other operations on the experimental animals; in addition, a waterer is provided on the mesh baffle, and a feeder is provided on the fixed baffle. Both the waterer and the feeder are detachable for easy cleaning and feeding.

[0036] In actual operation, the sum of the widths of the first sliding door 401 and the second sliding door 402 is greater than the width of the isolation cage 3 , that is, the first sliding door 401 and the second sliding door 402 have an overlapping portion, which further improves the mechanical isolation of the isolation cage 3 .

[0037] Specifically, the power ventilation unit includes an air supply system and an exhaust system. The air supply system includes an air supply fan 7 and an air supply duct 8. The air supply fan 7 is connected to the air supply duct 8. The air supply duct 8 is arranged between the box door 2 and the smooth door 4. The air supply duct 8 is provided with an air supply jet hole. The smooth door 4 is provided with an air inlet hole 9. The filtered clean air enters the air supply duct 8, enters the isolation box 1 through the air supply jet hole, and enters each isolation cage 3 through the air inlet hole 9. The exhaust system includes an exhaust fan 10 and an exhaust duct 11. The exhaust fan 10 is connected to the exhaust duct 11. The exhaust fan 10 is connected to the inner cavity of the isolation box 1. The exhaust duct 11 is connected to the external environment. The side wall of the isolation cage 3 opposite to the smooth door 4 is provided with an exhaust hole 12. The air in the isolation cage 3 enters the isolation box 1 through the exhaust hole 12, passes through the exhaust fan 10 and the exhaust duct 11, and is discharged outdoors after being filtered.

[0038] In this specific embodiment, there are two groups of isolation cages 3, and the air supply duct 8 is arranged between the two groups of isolation cages 3. The two groups of isolation cages 3 are symmetrically arranged with the axis of the air supply duct 8 as the center line, and multiple air supply jet holes are symmetrically arranged toward the isolation cages 3, and the air inlet hole 9 is arranged at one end of the smooth door 4 away from the air supply duct 8. Each group of isolation cages 3 is arranged from top to bottom, and the air supply duct 8 is arranged vertically. Affected by the small cross-section and long length of the air supply duct 8, it is easy to cause uneven air supply of the upper and lower isolation cages 3, which in turn causes a large difference in the pressure difference of each isolation cage 3. Therefore, along the air supply direction of the air supply duct 8, the diameter of the air supply jet hole gradually increases to meet the air supply volume demand of the isolation cage 3 located downstream of the air supply duct 8. The exhaust hole 12 is arranged on the back plate of the isolation cage 3, and the multiple exhaust holes 12 are arranged in a rectangular array to improve the uniformity of exhaust. The air inlet holes 9 are in two rows, and the two rows of air inlet holes 9 are staggered to improve the uniformity of air intake.

[0039] More specifically, the air inlet fan and the exhaust fan 10 are both arranged at the top of the isolation box 1, and the air filtration unit includes an air supply high efficiency filter 13, a heating module, an exhaust primary filter, a static pressure box and an exhaust high efficiency filter 14. The air supply high efficiency filter 13 and the heating module are both connected to the air supply fan 7, and the heating module can heat the air entering the isolation box 1. The static pressure box and the exhaust primary filter are arranged at the exhaust hole 12, and the exhaust high efficiency filter 14 is arranged between the exhaust primary filter and the exhaust fan 10. After the exhaust air is filtered by the exhaust primary filter, it is discharged to the exhaust high efficiency filter 14 through the static pressure box, and then discharged to the outside of the isolation box 1 through the exhaust fan 10 and the exhaust duct 11. It should be emphasized here that in order to facilitate the control of the pressure in the isolation box 1, a pressure control scheme of fixed delivery and variable exhaust is adopted. The air supply fan 7 and the exhaust fan 10 are both variable frequency fans. The air supply adopts constant air volume control, and the exhaust adopts variable air volume control, relying on changing the fan frequency. The negative pressure setting value in the isolation box 1 is -25Pa. When the isolation device is operating normally, the negative pressure difference can be guaranteed to be no less than -20Pa. At the same time, when the box door 2 is opened and the pressure is lost, the exhaust fan 10 will quickly increase the exhaust volume to ensure that the airflow at the box door 2 of the isolation box 1 is always flowing inward, effectively preventing the leakage of harmful gases.

[0040] Further, both the air supply high efficiency filter 13 and the exhaust high efficiency filter 14 are provided with a gas disinfection interface, which can be connected to the disinfection machine, and a closed valve and a closed cover are provided at the gas disinfection interface, and the disinfection gas of the disinfection machine can penetrate the air supply high efficiency filter 13, and be delivered to the isolation cage 3 through the air supply duct 8, and then penetrate the exhaust primary filter and the exhaust high efficiency filter 14, and reciprocate until the disinfection is completed. It should be noted that the air supply system and the exhaust system are both provided with a sealing valve for easy control. The air outlets of the air supply high efficiency filter 13 and the exhaust high efficiency filter 14 are both provided with an aerosol sampling port, which is convenient for leak detection using the efficiency method, specifically as follows, leak detection of the air supply high efficiency filter 13: aerosol is generated in the room where the isolation device is located, and after stabilization, the upstream aerosol concentration is detected at the entrance of the air supply fan 7 upstream of the air supply high efficiency filter 13, and then sampling is performed at the aerosol sampling point downstream of the air supply high efficiency filter 13, and the filtration efficiency of the air supply high efficiency filter 13 is calculated, and it is judged whether the air supply high efficiency filter 13 is intact and whether the installation is in place. Leak detection of exhaust high-efficiency filter 14: Aerosol is generated in the isolation cage 3. After stabilization, the upstream aerosol concentration is detected at the upstream sampling point of the exhaust high-efficiency filter 14, and then sampling is performed at the downstream aerosol sampling point of the exhaust high-efficiency filter 14 to calculate the filtration efficiency of the exhaust high-efficiency filter 14 and determine whether the exhaust high-efficiency filter 14 is intact and installed in place.

[0041] Furthermore, when the door 2 is closed, the clean air of the air supply system can form a protective airflow barrier between the door 2 and the smooth door 4. In order to prevent the jet air from the air supply jet hole from sucking the air inside the isolation device out of the isolation device when both the door 2 and the smooth door 4 are open, a sensor is provided at the door 2. The sensor can detect the opening and closing of the door 2. When the sensor senses that the door 2 is open, the control unit controls the air supply fan 7 to shut down and stop supplying air, thereby reducing the probability of the air inside the isolation device diffusing outward. The sensor, the air supply fan 7, and the exhaust fan 10 are all connected to the control unit. In addition, the isolation device is also provided with pressure and temperature monitoring elements, which are all connected to the control unit to facilitate the operator to monitor the status of the isolation device.

[0042] When the biosafety monkey negative pressure isolation device of the present invention is working, the isolation box 1 is in a negative pressure state through the control unit and the power ventilation unit. When the box door 2 and the smooth door 4 are opened, the directional flow of air can be effectively guaranteed to avoid the leakage of harmful gases in the isolation box 1; at the same time, a feces tray is arranged at the bottom of the isolation cage 3, and a sealing bag 5 is arranged outside the feces tray. When the feces tray needs to be taken out, the feces tray is directly placed in the sealing bag 5, and the sealing bag 5 is sealed and sterilized, effectively preventing the high concentration of harmful biological aerosols in the feces tray from leaking to the environment outside the isolator; in addition, the power ventilation unit is connected to an air filter unit, which can filter the gas entering and discharging the isolation box 1, realize the purpose of controlling the quality of air intake and exhaust, improve the operability of the isolation device, avoid harmful air discharge to the outside of the isolation box 1 to cause environmental pollution, and improve the safety of experimental personnel operation. The isolation device is also provided with a gas disinfection interface, which can realize the overall disinfection of the device.

[0043] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. At the same time, for those skilled in the art, according to the ideas of the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A biosafety monkey negative pressure isolation device, characterized in that: The invention comprises an isolation box, an air filtering unit, a power ventilation unit and a control unit, wherein the isolation box is provided with a box door, the box door is hingedly connected to the isolation box, a plurality of isolation cages are provided in the isolation box, the number of the isolation cages is consistent with the number of the box doors and corresponds one to one, the inner cavity of the isolation cage is connected to the inner cavity of the isolation box, the isolation cage can accommodate experimental animals, a smooth door is provided on the side of the box door close to the isolation cage, the smooth door is located between the box door and the isolation cage, the smooth door is slidably connected to the isolation box, a feces tray is provided at the bottom of the cage, the feces tray is connected to the isolation cage The inner cavity of the tool is connected, a sealing bag is connected to the bottom of the smooth door, the sealing bag is detachably connected to the smooth door, when the sealing bag is connected to the smooth door, a sealing element is arranged at the connection between the sealing bag and the smooth door, when the smooth door is closed, the sealing bag is arranged opposite to the feces tray, and the feces tray can enter the sealing bag; the isolation box is connected to the external environment through the power ventilation unit, the air filter unit is arranged between the power ventilation unit and the isolation box, the air filter unit can filter the airflow efficiently, and the air filter unit and the power ventilation unit are both connected to the control unit; The isolation cage has a fixed baffle and a mesh baffle on one side close to the smooth door, the mesh baffle is arranged on the top of the fixed baffle, an animal entrance and exit are opened on the fixed baffle, and the feces tray is arranged at the bottom of the animal entrance and exit; the box door and the smooth door are both made of transparent material, the smooth door includes a first sliding door and a second sliding door arranged in parallel, the first sliding door can block the animal entrance and exit, the distance between the first sliding door and the isolation cage is larger than the distance between the second sliding door and the isolation cage, and the sealing bag is detachably connected to the first sliding door; a connecting flange is arranged at the bottom of the first sliding door, the connecting flange can accommodate the passage of the feces tray, the sealing bag can be sleeved on the outside of the connecting flange, the outer side wall of the connecting flange has a sealing groove, when the sealing bag is sleeved on the outside of the connecting flange, a sealing ring is sleeved on the outside of the sealing bag, the sealing ring is located in the sealing groove, and the sealing bag is made of a flexible material; The powered ventilation unit comprises an air supply system and an exhaust system, the air supply system comprises an air supply fan and an air supply duct, the air supply fan is connected to the air supply duct, the air supply duct is arranged between the box door and the smooth door, the air supply duct is provided with air supply jet holes, the smooth door is provided with air inlet holes, the exhaust system comprises an exhaust fan and an exhaust duct, the exhaust fan is connected to the exhaust duct, the exhaust fan is connected to the inner cavity of the isolation box body, the exhaust duct is connected to the external environment, and exhaust holes are arranged on the side wall of the isolation cage opposite to the smooth door.

2. The biosafety monkey negative pressure isolation device according to claim 1, characterized in that: An internal baffle and a pull rod are arranged in the isolation cage, the internal baffle is slidably connected to the isolation cage, the internal baffle is arranged parallel to the fixed baffle, the internal baffle is connected to the pull rod, the pull rod is slidably connected to the fixed baffle, the pull rod has a plurality of slots, and the pull rod can be connected to the fixed baffle by means of the slots; a waterer is arranged on the grid baffle, and a feeder is arranged on the fixed baffle.

3. The biosafety monkey negative pressure isolation device according to claim 1 is characterized in that: There are two groups of isolation cages, the air supply duct is arranged between the two groups of isolation cages, the two groups of isolation cages are symmetrically arranged with the axis of the air supply duct as the center line, a plurality of air supply jet holes are symmetrically arranged toward the isolation cages, the air inlet hole is arranged at one end of the smooth door away from the air supply duct, and each group of isolation cages is stacked and arranged from top to bottom; a plurality of exhaust holes are arranged in a rectangular array.

4. The biosafety monkey negative pressure isolation device according to claim 3 is characterized in that: The air supply fan and the exhaust fan are both arranged on the top of the isolation box body, the air filtration unit includes an air supply high-efficiency filter, a heating module, an exhaust primary filter, a static pressure box and an exhaust high-efficiency filter, the air supply high-efficiency filter and the heating module are both connected to the air supply fan, the static pressure box and the exhaust primary filter are arranged at the exhaust hole, and the exhaust high-efficiency filter is arranged between the exhaust primary filter and the exhaust fan.

5. The biosafety monkey negative pressure isolation device according to claim 4 is characterized in that: The air supply high efficiency filter and the exhaust high efficiency filter are both provided with a gas disinfection interface, and the gas disinfection interface can be connected to a disinfection machine, and a closed valve and a closed cover are provided at the gas disinfection interface; the air outlets of the air supply high efficiency filter and the exhaust high efficiency filter are both provided with aerosol sampling ports.

6. The biosafety monkey negative pressure isolation device according to claim 5, characterized in that: A sensor is arranged at the door of the box, and the sensor can detect the opening and closing status of the door of the box. The sensor, the air supply fan and the exhaust fan are all connected to the control unit.

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