Emlical Extract Fermentation and Its Preparation and Application

Abstract

A composition comprising an extracted fermented product of Amla emblica, applications of the fermented product and its active ingredients, and a method for preparing the fermented product. Wherein, the emblica extract fermented product comprises at least one of the following compounds of formula (I) to formula (XIV):

Description

technical field

[0001] The present invention relates to a composition comprising at least one compound of the following formula (I) to formula (XIV) emblica extract fermented product, and a method for preparing the emblica extract fermented product:

[0002]

[0003]

[0004] The present invention is also about the application of at least one of the compound of formula (I) to formula (XIV) and the extracted fermented product of Amla emblica, especially about making the individual not easy to form body fat, whitening, anti-aging skin, and inhibiting the fat formation ability of fat cells , Promote the fat degradation ability of fat cells, promote weight loss, help metabolism, increase mitochondrial activity, inhibit melanin production, inhibit ROS production, inhibit AGEs production, increase SOD2 gene expression, increase MPG gene expression, and increase ERCC1 gene expression . Background technique

[0005] Obesity is a state of excess body fat accumulation. Under t...

Examples

preparation example Construction

[0046] The method for preparing emblica extract fermentation product according to the present invention comprises the following steps: (a) extracting emblica extract to provide an emblica extract; (b) fermenting the extract using a Saccharomyces cerevisiae and a Lactobacillus plantarum to obtain an intermediate fermented product; and (c) fermenting the intermediate fermented product with an acetic acid bacterium to obtain an emblica extract fermented product.

[0047] In step (a), a polar solvent can be used for extraction, preferably, the polar solvent is water, alcohol (such as C1-C4 alcohol), or a combination of the foregoing. In some embodiments of the present invention, water is used as the extraction solvent. Generally speaking, the amount of extraction solvent can be adjusted as needed, and there is no special limitation, as long as the raw materials can be evenly dispersed. For example, the weight ratio of emblica to extraction solvent can be used in step (a) in an am...

Embodiment 1

[0104] Example 1: Effect test of emblica extract and fermented product inhibiting adipogenesis of adipocytes

[0105] In this embodiment, the materials and equipment used are as follows:

[0106] 1. Cell line: OP9 (purchased from CRL-2749 TM ).

[0107] 2. Pre-adipocyte Expansion Medium:

[0108] 90% MEM-α medium (Minimum Essential Medium Alpha Medium, purchased from Gibco);

[0109] 20% fetal bovine serum (Fetal Bovine Serum, purchased from Gibco);

[0110] 1% Penicillin-streptomycin (Penicillin-streptomycin, purchased from Gibco).

[0111] 3. Adipocyte differentiation medium (Differentiation Medium):

[0112] 90% MEM-α medium (Minimum Essential Medium Alpha Medium, purchased from Gibco);

[0113] 20% fetal bovine serum (Fetal Bovine Serum, purchased from Gibco);

[0114] 1% Penicillin-streptomycin (Penicillin-streptomycin, purchased from Gibco).

[0115] 4. Phosphate buffered saline (PBS): purchased from Gibco

[0116] 5. Oil-red O staining reagent (Oil-red O stai...

Embodiment 2

[0130] Example 2: Effect test of emblica extract and fermentation product on enhancing mitochondrial activity

[0131] Mitochondria are the main place for energy generation in cells, and are closely related to cell information transmission and cell activity. The higher the activity of mitochondria, the more it can promote the overall physiological activities of cells. It is known that mitochondrial activity can be reflected by the measurement of mitochondrial membrane potential (MMP). In order to understand the effect of extracting and fermenting emblica of the present invention on enhancing mitochondrial activity, this example uses JC-1 mitochondrial stain to detect mitochondrial membrane potential, wherein, when the mitochondrial membrane potential increases, JC- 1 Mitochondrial dye will change from monomer with green fluorescent signal to dimer with red fluorescent signal. By using flow cytometry to detect the proportion of monomers and aggregates in the cells, the mitocho...

technical field

[0001] The present invention relates to a composition comprising at least one compound of the following formula (I) to formula (XIV) emblica extract fermented product, and a method for preparing the emblica extract fermented product:

[0002]

[0003]

[0004] The present invention is also about the application of at least one of the compound of formula (I) to formula (XIV) and the extracted fermented product of Amla emblica, especially about making the individual not easy to form body fat, whitening, anti-aging skin, and inhibiting the fat formation ability of fat cells , Promote the fat degradation ability of fat cells, promote weight loss, help metabolism, increase mitochondrial activity, inhibit melanin production, inhibit ROS production, inhibit AGEs production, increase SOD2 gene expression, increase MPG gene expression, and increase ERCC1 gene expression . Background technique

[0005] Obesity is a state of excess body fat accumulation. Under t...

Examples

preparation example Construction

[0046] The method for preparing emblica extract fermentation product according to the present invention comprises the following steps: (a) extracting emblica extract to provide an emblica extract; (b) fermenting the extract using a Saccharomyces cerevisiae and a Lactobacillus plantarum to obtain an intermediate fermented product; and (c) fermenting the intermediate fermented product with an acetic acid bacterium to obtain an emblica extract fermented product.

[0047] In step (a), a polar solvent can be used for extraction, preferably, the polar solvent is water, alcohol (such as C1-C4 alcohol), or a combination of the foregoing. In some embodiments of the present invention, water is used as the extraction solvent. Generally speaking, the amount of extraction solvent can be adjusted as needed, and there is no special limitation, as long as the raw materials can be evenly dispersed. For example, the weight ratio of emblica to extraction solvent can be used in step (a) in an am...

Embodiment 1

[0104] Example 1: Effect test of emblica extract and fermented product inhibiting adipogenesis of adipocytes

[0105] In this embodiment, the materials and equipment used are as follows:

[0106] 1. Cell line: OP9 (purchased from CRL-2749 TM ).

[0107] 2. Pre-adipocyte Expansion Medium:

[0108] 90% MEM-α medium (Minimum Essential Medium Alpha Medium, purchased from Gibco);

[0109] 20% fetal bovine serum (Fetal Bovine Serum, purchased from Gibco);

[0110] 1% Penicillin-streptomycin (Penicillin-streptomycin, purchased from Gibco).

[0111] 3. Adipocyte differentiation medium (Differentiation Medium):

[0112] 90% MEM-α medium (Minimum Essential Medium Alpha Medium, purchased from Gibco);

[0113] 20% fetal bovine serum (Fetal Bovine Serum, purchased from Gibco);

[0114] 1% Penicillin-streptomycin (Penicillin-streptomycin, purchased from Gibco).

[0115] 4. Phosphate buffered saline (PBS): purchased from Gibco

[0116] 5. Oil-red O staining reagent (Oil-red O stai...

Embodiment 2

[0130] Example 2: Effect test of emblica extract and fermentation product on enhancing mitochondrial activity

[0131] Mitochondria are the main place for energy generation in cells, and are closely related to cell information transmission and cell activity. The higher the activity of mitochondria, the more it can promote the overall physiological activities of cells. It is known that mitochondrial activity can be reflected by the measurement of mitochondrial membrane potential (MMP). In order to understand the effect of extracting and fermenting emblica of the present invention on enhancing mitochondrial activity, this example uses JC-1 mitochondrial stain to detect mitochondrial membrane potential, wherein, when the mitochondrial membrane potential increases, JC- 1 Mitochondrial dye will change from monomer with green fluorescent signal to dimer with red fluorescent signal. By using flow cytometry to detect the proportion of monomers and aggregates in the cells, the mitocho...

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