Fermentation product of lotus leaf-hawthorn extract and application of active ingredients of fermentation product to inhibition of fat formation

Abstract

Use of at least one of the following compounds of formula (I) to formula (III) is provided. Provided is a composition comprising a fermentation product of lotus leaf-hawthorn extract that can provide at least one of the above compounds of formula (I) to formula (III), and also provided is a method for preparing the fermentation product of lotus leaf-hawthorn extract.

Description

technical field

[0001] The present invention relates to the application of at least one of the compounds of the following formula (I) to formula (III), especially related to the application of the above compounds in promoting the fat degradation ability of adipocytes, inhibiting the adipogenesis ability of adipocytes, making it difficult for individuals to form body fat, and / or apps that promote weight loss:

[0002]

[0003] The present invention further relates to a composition comprising a fermented product of a hawthorn extract capable of providing at least one of the above-mentioned compounds of formula (I) to formula (III), and a method for preparing the fermented product of a hawthorn extract. Background technique

[0004] Obesity is a state of excess body fat. Internationally, body mass index (BMI) or waist circumference are usually used as indicators for evaluating obesity. In Taiwan, China, a BMI value greater than or equal to 24 and less than 27 indicates ove...

Examples

Embodiment 1

[0125] Example 1: The effect of the fermented product of lotus leaf hawthorn extract on reducing the fat content of adipocytes

[0126] Take the adipocytes provided in [Preparation Example C], divide them into three groups and culture them with the following medium respectively, at 37°C for 7 days, during which the medium is replaced every 3 days:

[0127] 1. Group I: adipocyte differentiation medium (500 microliters in total).

[0128] 2. Group II: adipocyte differentiation medium containing 5% (weight / weight) of the lotus leaf Hawthorn extract provided in [Preparation Example A-1] (500 microliters in total); and

[0129] 3. Group III: adipocyte differentiation medium containing 5% (weight / weight) of the fermented product of the lotus leaf hawthorn extract provided in [Preparation Example A-3] (500 microliters in total);

[0130] Afterwards, the cells provided by groups I to III were treated with staining and quantification steps as follows: medium was removed and cells were...

Embodiment 2

[0133] Example 2: The effect of different partitioning of the fermented product of lotus leaf hawthorn extract on reducing the fat content of adipocytes

[0134] Take the adipocytes provided in [Preparation Example C], divide them into four groups and culture them with the following medium respectively, at 37°C for 7 days, during which the medium is replaced every 3 days:

[0135] 1. Group i: adipocyte differentiation medium (500 microliters in total);

[0136] 2. Group ii: adipocyte differentiation medium containing 5% (weight / weight) of the fermented product of the lotus-leaf Hawthorn extract provided in [Preparation Example A-3] (500 microliters in total);

[0137] 3. Group iii: adipocyte differentiation medium containing 5% (weight / weight) of the n-butanol layer extract provided in [Preparation Example B-1] (500 microliters in total);

[0138] 4. Group iv: adipocyte differentiation medium containing 5% (weight / weight) of the aqueous layer extract provided in [Preparation ...

Embodiment 3

[0141] Embodiment 3: the effect of formula (I) to formula (III) compound in reducing the fat content of adipocytes

[0142] Take the adipocytes provided in [Preparation Example C], divide them into four groups and culture them with the following medium respectively, at 37°C for 7 days, during which the medium is replaced every 3 days:

[0143] 1. Group A: adipocyte differentiation medium (500 microliters in total);

[0144] 2. Group B: adipocyte differentiation medium containing 20 micrograms / ml [Preparation Example B-3] of the compound of formula (I) provided (500 microliters in total);

[0145] 3. Group C: the adipocyte differentiation medium (500 microliters in total) containing the compound of formula (II) provided by 20 micrograms / ml [Preparation Example B-3];

[0146] 4. Group D: adipocyte differentiation medium containing 20 μg / ml of the compound of formula (III) provided in [Preparation Example B-3] (500 μl in total).

[0147] Afterwards, the cells provided in Groups...

technical field

[0001] The present invention relates to the application of at least one of the compounds of the following formula (I) to formula (III), especially related to the application of the above compounds in promoting the fat degradation ability of adipocytes, inhibiting the adipogenesis ability of adipocytes, making it difficult for individuals to form body fat, and / or apps that promote weight loss:

[0002]

[0003] The present invention further relates to a composition comprising a fermented product of a hawthorn extract capable of providing at least one of the above-mentioned compounds of formula (I) to formula (III), and a method for preparing the fermented product of a hawthorn extract. Background technique

[0004] Obesity is a state of excess body fat. Internationally, body mass index (BMI) or waist circumference are usually used as indicators for evaluating obesity. In Taiwan, China, a BMI value greater than or equal to 24 and less than 27 indicates ove...

Examples

Embodiment 1

[0125] Example 1: The effect of the fermented product of lotus leaf hawthorn extract on reducing the fat content of adipocytes

[0126] Take the adipocytes provided in [Preparation Example C], divide them into three groups and culture them with the following medium respectively, at 37°C for 7 days, during which the medium is replaced every 3 days:

[0127] 1. Group I: adipocyte differentiation medium (500 microliters in total).

[0128] 2. Group II: adipocyte differentiation medium containing 5% (weight / weight) of the lotus leaf Hawthorn extract provided in [Preparation Example A-1] (500 microliters in total); and

[0129] 3. Group III: adipocyte differentiation medium containing 5% (weight / weight) of the fermented product of the lotus leaf hawthorn extract provided in [Preparation Example A-3] (500 microliters in total);

[0130] Afterwards, the cells provided by groups I to III were treated with staining and quantification steps as follows: medium was removed and cells were...

Embodiment 2

[0133] Example 2: The effect of different partitioning of the fermented product of lotus leaf hawthorn extract on reducing the fat content of adipocytes

[0134] Take the adipocytes provided in [Preparation Example C], divide them into four groups and culture them with the following medium respectively, at 37°C for 7 days, during which the medium is replaced every 3 days:

[0135] 1. Group i: adipocyte differentiation medium (500 microliters in total);

[0136] 2. Group ii: adipocyte differentiation medium containing 5% (weight / weight) of the fermented product of the lotus-leaf Hawthorn extract provided in [Preparation Example A-3] (500 microliters in total);

[0137] 3. Group iii: adipocyte differentiation medium containing 5% (weight / weight) of the n-butanol layer extract provided in [Preparation Example B-1] (500 microliters in total);

[0138] 4. Group iv: adipocyte differentiation medium containing 5% (weight / weight) of the aqueous layer extract provided in [Preparation ...

Embodiment 3

[0141] Embodiment 3: the effect of formula (I) to formula (III) compound in reducing the fat content of adipocytes

[0142] Take the adipocytes provided in [Preparation Example C], divide them into four groups and culture them with the following medium respectively, at 37°C for 7 days, during which the medium is replaced every 3 days:

[0143] 1. Group A: adipocyte differentiation medium (500 microliters in total);

[0144] 2. Group B: adipocyte differentiation medium containing 20 micrograms / ml [Preparation Example B-3] of the compound of formula (I) provided (500 microliters in total);

[0145] 3. Group C: the adipocyte differentiation medium (500 microliters in total) containing the compound of formula (II) provided by 20 micrograms / ml [Preparation Example B-3];

[0146] 4. Group D: adipocyte differentiation medium containing 20 μg / ml of the compound of formula (III) provided in [Preparation Example B-3] (500 μl in total).

[0147] Afterwards, the cells provided in Groups...

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