Sampling structure, sealing structure and detection components

Abstract

A sampling structure, a sealing structure and a detection component. The sampling structure includes a first body, a second body and a third body. The first body includes a first channel, and the first channel includes a first opening exposed to the outside. The second body is connected to the first body, including a second channel and at least one partition column located in the second channel, the second channel is communicated with the first channel, and a first gap is provided between the partition column and the channel wall of the second channel. The third body is connected to the second body, including a chamber, which is communicated with the second channel and can accommodate a sample. The sampling structure can absorb a large amount of sample through the first channel and the second channel, and can make the sample fully mixed in the chamber.

Description

Technical Field

[0001] Embodiments of the present disclosure relate to a sampling structure, a sealing structure, and a detection assembly. Background Art

[0002] Microfluidic chip technology integrates the basic operating units such as sample preparation, reaction, separation, and detection involved in the fields of biology, chemistry, and medicine onto a chip with micron-scale microchannels, automatically completing the entire process of reaction and analysis. The chip used in this process is called a microfluidic chip, also known as a chip laboratory (Lab-on-a-chip). Microfluidic chip technology has the advantages of small sample usage, fast analysis speed, easy to make portable instruments, and suitable for instant and on-site analysis. It has been widely used in many fields such as biology, chemistry, and medicine. Summary of the invention

[0003] At least one embodiment of the present disclosure provides a sampling structure, including a first body, a second body and a third body. The first body includes a first channel, and the first channel includes a first opening exposed to the outside. The second body is connected to the first body, including a second channel and at least one partition column located in the second channel, wherein the second channel is connected to the first channel, and there is a first gap between the partition column and the channel wall of the second channel. The third body is connected to the second body, including a chamber, wherein the chamber is connected to the second channel and can accommodate a sample.

[0004] For example, in the sampling structure provided in at least one embodiment of the present disclosure, the at least one partition column includes a plurality of partition columns, the first channel includes a second opening connected to the second channel, and the plurality of partition columns are spaced apart from each other around the second opening.

[0005] For example, in the sampling structure provided in at least one embodiment of the present disclosure, the plurality of partition columns are centrally symmetrically arranged around the central axis of the second opening.

[0006] For example, in the sampling structure provided in at least one embodiment of the present disclosure, each of the partition columns at least partially overlaps with the second opening.

[0007] For example, in the sampling structure provided in at least one embodiment of the present disclosure, the cross-section of each of the partition columns is fan-shaped.

[0008] For example, in the sampling structure provided in at least one embodiment of the present disclosure, a plurality of the partition columns are arranged relative to each other and the resulting shape is cylindrical as a whole.

[0009] For example, in the sampling structure provided in at least one embodiment of the present disclosure, there is a second gap between adjacent separation columns, and the width of the second gap is 0.1 mm-2.5 mm.

[0010] For example, in the sampling structure provided in at least one embodiment of the present disclosure, the radius of the first channel is 0.1 mm-3 mm, and the radius of the second channel is 1 mm-10 mm.

[0011] For example, in the sampling structure provided in at least one embodiment of the present disclosure, the cross-section of each of the partition columns is fan-shaped, and the radius of the fan-shaped is 0.5 mm-8 mm.

[0012] For example, in the sampling structure provided in at least one embodiment of the present disclosure, the length of the first channel is 0.1 mm-10 mm, and the length of the second channel is 1 mm-10 mm.

[0013] For example, in the sampling structure provided in at least one embodiment of the present disclosure, the first body, the second body and the third body are an integrated structure.

[0014] At least one embodiment of the present disclosure provides a sealing structure, which includes a sealing portion, and the sealing portion includes an elastic main body; wherein the sealing portion includes at least one exhaust hole, and the exhaust hole is configured to be in an open state or a closed state when the main body is subjected to different forces.

[0015] For example, the sealing structure provided in at least one embodiment of the present disclosure further includes a fixing portion, which is fixedly connected to the sealing portion and includes a fixing structure.

[0016] For example, in the sealing structure provided in at least one embodiment of the present disclosure, the fixing structure is an annular sleeve structure.

[0017] For example, in the sealing structure provided by at least one embodiment of the present disclosure, when the force applied to the main body is less than a threshold value, the exhaust hole is in the open state; when the force applied to the main body is greater than or equal to the threshold value, the exhaust hole is in the closed state.

[0018] For example, in the sealing structure provided in at least one embodiment of the present disclosure, the exhaust hole is a triangular prism exhaust hole, including a first wall and a second wall connected to the main body, the first wall and the second wall are connected, and two opposite triangular openings are formed between the first wall and the second wall and the main body, and when the force acting on the main body is greater than or equal to the threshold, the first wall and the second wall can be stretched to be located in the same plane, so that the triangular opening is closed and the exhaust hole is in the closed state.

[0019] For example, in the sealing structure provided in at least one embodiment of the present disclosure, the sealing portion also includes a raised portion for sealing, the raised portion is arranged around the main body portion, and the longitudinal cross-section of the raised portion is a shape that gradually narrows from the first end to the second end, wherein the first end is an end connected to the main body portion, and the second end is an opposite end to the first end.

[0020] For example, in the sealing structure provided in at least one embodiment of the present disclosure, the fixing portion and the sealing portion are an integral structure.

[0021] At least one embodiment of the present disclosure provides a detection component, including a microfluidic chip, any of the above-mentioned sampling structures and a sealing structure. The microfluidic chip includes a sampling slot and a sampling structure mounting portion, the sampling structure mounting portion is connected to the sampling slot, the sampling structure can be set in the sampling slot, and the sealing structure can be installed in the sampling structure mounting portion to seal the sampling structure.

[0022] For example, in the detection component provided in at least one embodiment of the present disclosure, the sealing structure is any of the above-mentioned sealing structures, and the microfluidic chip also includes a sealing structure mounting part, wherein the sealing part of the sealing structure can be mounted on the sampling structure mounting part, and the fixing part of the sealing structure can be mounted on the sealing structure mounting part.

[0023] For example, in the detection assembly provided in at least one embodiment of the present disclosure, the fixing structure of the sealing structure is an annular sleeve structure, and the sealing structure mounting portion includes an annular groove that cooperates with the annular sleeve structure.

[0024] For example, in the detection assembly provided in at least one embodiment of the present disclosure, the sealing portion of the sealing structure further includes a protrusion for sealing, and the sampling structure mounting portion includes a slot that matches the protrusion.

[0025] For example, the detection assembly provided in at least one embodiment of the present disclosure further includes a push rod, wherein the push rod is movably arranged to apply a force to the main body.

[0026] For example, in the detection assembly provided in at least one embodiment of the present disclosure, the main body also includes a concave groove that cooperates with the push rod to guide the force application position of the push rod. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below. Obviously, the drawings in the following description only relate to some embodiments of the present disclosure, but are not intended to limit the present disclosure.

[0028] Figure 1 A cross-sectional view of a sampling structure provided for at least one embodiment of the present disclosure;

[0029] Figure 2 A top view of a sampling structure provided for at least one embodiment of the present disclosure;

[0030] Figure 3 A three-dimensional diagram of a packaging structure provided by at least one embodiment of the present disclosure;

[0031] Figure 4 A cross-sectional view of a packaging structure provided by at least one embodiment of the present disclosure;

[0032] Figure 5 A three-dimensional diagram of a sealing portion of a packaging structure provided by at least one embodiment of the present disclosure;

[0033] Figure 6 A schematic diagram of the cooperation between the sampling structure and the packaging structure provided in at least one embodiment of the present disclosure;

[0034] Figure 7 A three-dimensional diagram of a detection assembly provided for at least one embodiment of the present disclosure;

[0035] Figure 8 A perspective view of another detection assembly provided for at least one embodiment of the present disclosure;

[0036] Fig. 9 A partial cross-sectional view of a detection assembly provided for at least one embodiment of the present disclosure;

[0037] Fig.10 A working principle diagram of a detection component provided for at least one embodiment of the present disclosure;

[0038] Fig.11 Another working principle diagram of the detection component provided for at least one embodiment of the present disclosure. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical solution and advantages of the embodiments of the present disclosure clearer, the technical solution of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0040] Unless otherwise defined, the technical terms or scientific terms used in the present disclosure should be understood by people with ordinary skills in the field to which the present disclosure belongs. The "first", "second" and similar words used in the present disclosure do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word cover the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0041] In the design process of microfluidic chip, it is usually hoped that as many functions of analysis and detection as possible are integrated into the chip to reduce the dependence of the chip on external operations, thereby realizing automation and integration. For example, the sampling component, mixing component and analysis and detection component of the microfluidic chip can be integrated together to realize the automation of the detection process. In the process of detection by the microfluidic chip, the sampling component is first used to obtain the sample to be detected, and then the sample to be detected and the detection reagent (or diluent, etc., which makes the sample to be detected more suitable for detection) are fully mixed in the mixing component for the next detection operation. The mixing effect of the detection sample and the detection reagent plays a vital role in the detection process and the detection result of the microfluidic chip.

[0042] At least one embodiment of the present disclosure provides a sampling structure, including a first body, a second body and a third body. The first body includes a first channel, and the first channel includes a first opening exposed to the outside. The second body is connected to the first body, including a second channel and at least one separator column located in the second channel, wherein the second channel is connected to the first channel, and there is a first gap between the separator column and the channel wall of the second channel. The third body is connected to the second body, including a chamber, which is connected to the second channel and can accommodate a sample.

[0043] At least one embodiment of the present disclosure provides a sealing structure, which includes a sealing portion, and the sealing portion includes an elastic main body; the sealing portion includes at least one exhaust hole, and the exhaust hole is configured to be in an open state or a closed state when the main body is subjected to different forces.

[0044] At least one embodiment of the present disclosure further provides a detection assembly, including a microfluidic chip, the above-mentioned sampling structure and a sealing structure. The microfluidic chip includes a sampling slot and a sampling structure mounting portion, the sampling structure mounting portion is communicated with the sampling slot, the sampling structure can be arranged on the sampling structure mounting portion, and the sealing structure can be installed on the sampling structure mounting portion to seal the sampling structure.

[0045] The sampling structure, sealing structure and detection assembly of the present disclosure are described below through several specific embodiments.

[0046] At least one embodiment of the present disclosure provides a sampling structure, such as Figure 1 and Figure 2 As shown, the sampling structure includes a first body 101, a second body 102 and a third body 103. The first body 101 includes a first channel 1011, and the first channel 1011 includes an exposed first opening 1012, and the first opening 1012 is used to communicate with the outside. The second body 102 is connected to the first body 101, and includes a second channel 1021 and at least one partition column 1022 located in the second channel. The second channel 102 is connected to the first channel 101, and a first gap L1 is provided between the partition column 1022 and the channel wall 1023 of the second channel 1021. The third body 103 is connected to the second body 102, and includes a chamber 1031, and the chamber 1031 is connected to the second channel 1021 and can accommodate a sample.

[0047] The first channel 1011 and the second channel 1021 of the above-mentioned structure can be configured to absorb the sample to be detected (such as blood, body fluid, etc.) through capillary action, and the second channel 1021 has a partition column 1022, which is arranged in the second channel 1021, thereby diverting the absorbed sample to be detected flowing through the second channel 1021. In the chamber 1031, when the sample is mixed with the detection reagent, the sampling structure can achieve the effect of sample mixing, so that the sampling structure integrates the absorption and mixing functions and has multifunctionality. The sampling structure can be used in detection devices such as microfluidic chips, for example.

[0048] For example, in some embodiments, the diameter of the second channel 1021 is greater than the diameter of the first channel 101, which is beneficial to the arrangement of the partition column 1022 in the second channel 1021 and can also increase the amount of the sample to be detected that can be absorbed by the second channel 1021. The chamber 1031 can be cylindrical in shape as a whole, with a diameter greater than the diameter of the second channel 1021.

[0049] It should be noted that in at least one embodiment of the present disclosure, "diameter" refers to the main dimension of the channel in the cross section. For example, when the cross section of the channel is circular, the diameter refers to the diameter of the circle (i.e., the case shown in the figure); when the cross section of the channel is square, the diameter refers to the diagonal of the square; when the cross section of the channel is rectangular, the diameter refers to the diagonal of the rectangle, that is, "diameter" can be understood as a representative dimension that passes through the center of the cross section of the channel.

[0050] For example, in some embodiments, Figure 1 and Figure 2 As shown, the second channel 1021 has at least one partition column 1022, including one partition column, or a plurality of partition columns 1022, for example, two, three, four (as shown in the figure) or five partition columns 1022, etc. The number of partition columns 1022 can be selected according to actual needs.

[0051] For example, the first channel 1011, the second channel 1021 and the chamber 1031 are parallel, that is, along the same direction ( Figure 1 The first channel 1011 extends in a vertical direction (in a vertical direction), the first channel 1011 includes a second opening 1013 connected to the second channel 1021, and a plurality of partition columns 1022 are arranged around the second opening 1013. The second channel 1021 has a third opening connected to the chamber 1031. For example, in the extension direction, the projection of the second opening 1013 of the first channel 1011 is located in the cross section of the second channel 1021, and the projection of the third opening of the second channel 1021 is located in the cross section of the chamber 1031. For example, the first channel 1011, the second channel 1021 and the chamber 1031 have the same central axis, for example, the first channel 1011, the second channel 1021 and the chamber 1031 are centrally symmetrical around the central axis.

[0052] For example, there is a first gap between the partition column 1022 and the channel wall 1023 of the second channel 1021, and there is a second gap L2 between adjacent partition columns 1022, thereby, the first gap L1 and the second gap L2 can promote the absorption process of the sample to be detected through capillary action. In addition, when the sample to be detected is mixed with the detection reagent, the first gap L1 and the second gap L2 form a plurality of shunt channels, which can make the sample to be detected and the detection reagent mixed more fully (details later). The length of the partition column 1022 can be less than or equal to the length of the second channel 1021, thereby the upper end surface of the partition column 1022 can be flush with the bottom surface of the chamber 1031, or spaced a predetermined distance from the bottom surface of the chamber 1031.

[0053] For example, in some embodiments, the plurality of partition columns 1022 are centrally symmetrically arranged around the central axis of the second opening 1013 so that the second channel 1021 at different positions has the same or similar degree of suction and mixing effects.

[0054] For example, in some embodiments, Figure 1 and Figure 2 As shown, each partition column 1022 at least partially overlaps with the second opening 1013, that is, in the extension direction of the second channel 1021, the projection of each partition column 1022 overlaps with the second opening 1013. For example, each partition column 1022 partially blocks the second opening 1013, so that the sample to be detected drawn from the first channel 1011 can fully enter the first gap L1 and the second gap L2 in the second channel 1021, so as to achieve the effects of drawing and diversion through the first gap L1 and the second gap L2.

[0055] For example, in some embodiments, Figure 1 and Figure 2 As shown, the first channel 1011 and the second channel 1021 can be cylindrical, that is, a circular channel. The cross-section of each separator 1022 can be a variety of appropriate shapes, such as a fan-shaped. For example, a plurality of separators 1022 are arranged relative to each other and the shape obtained by the arrangement is cylindrical as a whole (that is, the overall shape is circular). At this time, the first gap L1 between each separator 1022 and the channel wall 1023 of the second channel 1021 is basically the same, and the second gap L2 between each two adjacent separators 1022 is also basically the same, thereby further improving the effect of uniform absorption and diversion of the second channel 1021.

[0056] For example, in some embodiments, the structural parameters of each part of the sampling structure can be designed to better realize the functions of the sampling structure. For example, the width of the second gap L2 between adjacent partitions 1022 is 0.1 mm-2.5 mm, such as 0.2 mm, 0.5 mm, 1 mm or 2 mm.

[0057] For example, in some embodiments, the radius R1 of the first channel 1011 is 0.1mm-3mm, such as 0.3mm, 0.5mm, 0.6mm, 1mm or 2mm, etc., and the radius R2 of the second channel 1021 is greater than the radius R1 of the first channel, for example, it can be 1mm-10mm, such as 2mm, 4mm, 6mm or 8mm, etc.

[0058] For example, in some embodiments, when the cross section of each partition column 1022 is fan-shaped, the radius R3 of the fan-shaped is 0.5 mm-8 mm, for example, 1.5 mm, 2.5 mm, 3.5 mm, 4.5 mm, 5.5 mm or 6.5 mm.

[0059] For example, in some embodiments, the length H1 of the first channel 1011 is 0.1mm-10mm, such as 0.5mm, 1mm, 1.5mm, 3mm, 5mm or 8mm, and the length H2 of the second channel 1021 is 1mm-10mm, such as 3mm, 5mm, 7mm or 9mm.

[0060] At this time, the amount of sample that can be absorbed by the first channel 1011 is V1 = π×(R1) 2 ×(H1), the amount of sample that can be absorbed by the second channel 1021 is V2 is π×[(R2) 2 -(R3) 2 ]×(H2), so the amount V of sample that can be absorbed by the sampling structure is at least equal to V1+V2.

[0061] Since the first channel 1011 and the second channel 2011 of the sampling structure can absorb samples together, the total amount of sample absorbed by the sampling structure is larger. In other words, when the same total amount of sample is absorbed, the height of the sampling structure is lower. Therefore, it can be used in the case where the microfluidic biochip is relatively thin, that is, the sampling structure is easier to match with the microfluidic biochip.

[0062] In addition, in the above-mentioned sampling structure, since the first channel 1011 and the second channel 2011 are relatively thin, when the sample to be detected is mixed with the detection reagent and passes through the first channel 1011 and the second channel 2011 into the chamber 1031, the flow rate of the mixed liquid is accelerated, and then the mixed liquid can rush into the chamber 1031 at a higher speed, forming a vortex mixing, thereby improving the mixing efficiency.

[0063] For example, in some embodiments, the first body 101, the second body 102 and the third body 103 of the sampling structure may be an integrated structure. In this case, during the preparation process, the sampling structure may be manufactured in one process to simplify the manufacturing process. For example, the sampling structure may be manufactured in an integrated injection molding process.

[0064] For example, in some embodiments, the material of the sampling structure may be polymethyl methacrylate (PMMA), polystyrene (PS), polycarbonate (PC), or polypropylene (PP), etc. For example, the sampling structure may be manufactured by an upper and lower demolding injection molding process, which includes, for example, filling the material of the sampling structure into a mold by pressurizing, injecting, etc., and then cooling the material, separating the material from the mold, etc. The manufacturing process is simple, can improve productivity, and save costs.

[0065] At least one embodiment of the present disclosure further provides a sealing structure, such as Figure 3As shown, the sealing structure includes a sealing portion 201, the sealing portion 201 includes an elastic main body 2011, and the main body 2011 may include an elastic diaphragm with a predetermined thickness; the sealing portion 201 includes at least one exhaust hole, and the exhaust hole is configured to be in an open state or a closed state when the main body 2011 is subjected to different forces.

[0066] For example, in some embodiments, the sealing structure may further include a fixing portion 202. The fixing portion 202 is fixedly connected to the sealing portion 201 and includes a fixing structure 2021. The sealing structure may be used to fix and seal the above-mentioned sampling structure, for example.

[0067] For example, Figure 3 As shown, the fixing structure 2021 may be an annular sleeve structure. For example, the annular sleeve structure includes an annular protrusion, which may be fixed to another structure (such as an annular groove) that matches the fixing structure 2021 by sleeve connection. The fixing portion 202 and the sealing portion 201 may be connected to each other by a connecting portion 203 disposed therebetween, or the fixing portion 202 and the sealing portion 201 may be directly connected.

[0068] For example, Figure 4 A cross-sectional schematic diagram of a sealing structure sealed to a sampling structure is shown. For example, the sealing portion 202 of the sealing structure is used to fix the sampling structure to a device (such as a microfluidic chip, which will be described in detail later), and the sealing portion 201 of the sealing structure is used to seal the sampling structure.

[0069] For example, Figure 5 A schematic diagram of the structure of the sealing part is shown, and Figure 5 The bottom of the sealing portion 202 (eg Figure 4 The stereogram is observed from the lower side of the image. Figure 5 As shown, the sealing portion includes at least one exhaust hole 2012, for example, the main body 2011 of the sealing portion includes at least one exhaust hole 2012 at a peripheral position, for example, includes a plurality of exhaust holes 2012, and these exhaust holes are evenly distributed along the sealing portion 201. The exhaust hole 2012 is configured to be in an open state or a closed state when the main body 2011 is subjected to different forces, so that the internal pressure of the sampling structure can be adjusted.

[0070] For example, in some embodiments, Figure 3 As shown, the push rod 2014 can be used to abut against the main body 2011 to apply a force to the main body 2011, thereby operating the sampling structure. For example, when the force applied to the main body 2011 is less than a threshold, the exhaust hole 2012 is in an open state; when the force applied to the main body 2011 is greater than or equal to the threshold, the exhaust hole 2012 is in a closed state. Thus, the exhaust hole 2012 can realize a one-way valve function.

[0071] For example, in some embodiments, Figure 5 As shown, the exhaust hole 2012 may be a triangular prism exhaust hole, including a first wall 2012A and a second wall 2012B connected to the main body 2011, the first wall 2012A and the second wall 2012B are connected, and two opposite triangular openings 2012C are formed between the first wall 2012A and the second wall 2012B and the main body 2011. When the force applied to the main body 2011 is greater than or equal to a threshold value, the first wall 2012A and the second wall 2012B may be stretched to be located in the same plane, so that the triangular opening 2012C is closed, and the exhaust hole 2012 is in a closed state. When the force applied to the main body 2011 is greater than the threshold value, the exhaust hole 2012 is in an open state, and at this time, the first wall 2012A, the second wall 2012B and the triangular opening 2012C may form a triangular prism shape.

[0072] For example, Figure 6 A schematic diagram showing the main body 2011 and the sampling structure being installed in coordination. Figure 6 As shown by the thick arrow in the figure, during the operation, a gradually increasing force can be applied to the main body 2011 by the push rod (not shown in the figure), and the main body 2011 is forced to move downward to squeeze the gas in the chamber of the sampling structure. When the force applied to the main body 2011 is less than the threshold value, the exhaust hole 2012 is in an open state, and the gas in the chamber of the sampling structure can be discharged through the exhaust hole 2012, as shown by the thin arrow in the figure, so that the air pressure in the chamber of the sampling structure does not change. When the force applied to the main body 2011 is greater than or equal to the threshold value, the exhaust hole 2012 is in a closed state, and the main body 2011 is forced to continue to move downward, so that the air pressure in the sampling structure increases. At this time, the sample in the chamber or channel of the sampling structure can be pushed out. Therefore, through the design of the exhaust hole, it is possible to avoid excessive increase in the internal pressure of the sampling structure in a short period of time, so that the sample push speed is too fast and the push amount of the sample cannot be controlled.

[0073] For example, in some embodiments, Figure 4 As shown, the sealing portion 201 may further include a protrusion 2013 for sealing, and the protrusion 2013 is arranged around the main body 2011, and its plan view forms a ring. Figure 3 and Figure 4The main body 2011 includes a step portion at the periphery, and the diaphragm portion of the main body 2011 is connected to the protrusion 2013 through the step portion. In addition, the longitudinal section of the protrusion 2013 is in a shape that gradually narrows from the first end 2013A to the second end 2013B. The first end 2013A is an end connected to the main body 2011 (shown as the upper end in the figure), and the second end 2013B is the opposite end of the first end 2013A (shown as the lower end in the figure). For example, the width of the second end 2013B can be 0.5mm-5mm, for example, 2mm, 3mm or 4mm, etc., and the inclination angle of the side wall of the protrusion 2013 (relative to the vertical direction in the figure) can be 2°-20°, for example, 10°, 14° or 18°, etc. For example, in some embodiments, the exhaust hole 2012 can be opened on the inner side wall of the protrusion 2013, such as Figure 4 shown.

[0074] For example, in some embodiments, the fixing part 202 and the sealing part 201 are an integrated structure, and are made of an elastic material, and the elastic material can be a synthetic material or a natural material. For example, the fixing part and the sealing part are an integrated silicone structure, and the silicone structure has high elasticity and can better achieve the sealing function. In this case, in the manufacturing process, the fixing part 202 and the sealing part 201 can be formed in one piece to simplify the manufacturing process and improve the yield.

[0075] At least one embodiment of the present disclosure provides a detection component, such as Figure 7 As shown, the detection component includes a microfluidic chip 300 , any of the above-mentioned sampling structures and a sealing structure 200 .

[0076] For example, the microfluidic chip 300 includes a chip main body 310 and a chip interface 320. The chip interface 320 is arranged on one side of the chip main body 310, and includes a sampling structure mounting component 301; the chip main body 310 includes a channel and a detection structure communicating with the chip interface 320, for example, a driving electrode is arranged in the channel, and the sample is driven by the electrowetting effect on the medium, and the detection structure may include a photoelectric detection device, etc. These structures may refer to existing microfluidic chips, and will not be described in detail here.

[0077] because Figure 7 FIG. 1 is a schematic diagram of the detection component after assembly. At this time, the sampling structure has been installed in the sampling structure installation component 301 of the microfluidic chip 300. Therefore, for simplicity, Figure 7 The sampling structure is not shown in detail.

[0078] For example, Fig. 9 FIG. 3 shows a cross-sectional schematic diagram of a sampling structure installation component 301, in which the sampling structure is arranged. Fig. 9As shown, the sampling structure installation component 301 includes a sampling slot 302 and a sampling structure installation portion 303. The sampling structure installation portion 303 is communicated with the sampling slot 302. The sampling structure 100 can be set in the sampling slot 302. The sealing structure 200 can be installed on the sampling structure installation portion 303 to seal the sampling structure 100.

[0079] For example, the microfluidic chip 300 also includes a reagent pool 305 for storing detection reagents. After the sampling structure 100 is installed in the microfluidic chip 300, the sampling structure 100 extends into the reagent pool 305, so that the sample to be detected can be mixed with the detection reagent in the reagent pool 305. For example, the microfluidic chip 300 also includes a microfluidic channel that can be communicated with the reagent pool 305 and a detection component (not shown in the figure) connected to the microfluidic channel. After the sample to be detected is mixed with the detection reagent in the reagent pool 305, the mixed solution can be transported to the detection component through the microfluidic channel for detection. For example, the detection component can be multiple, and is connected to the reagent pool 305 through the microfluidic channel respectively, thereby realizing multiple detections of the sample to be detected at the same time.

[0080] For example, in some embodiments, the mixing ratio of the sample to be detected and the detection reagent can be certain, so the volume of the detection reagent in the reagent pool 305 is certain, that is, the height of the detection reagent in the reagent pool 305 is certain. At this time, the lower structure of the sampling mechanism provided in the embodiment of the present disclosure, such as the design of the first channel and the second channel, can better adapt to the liquid level height of the detection reagent in the reagent pool 305, such as the low liquid level height.

[0081] For example, in some embodiments, the sealing structure 200 is the sealing structure provided by the above-mentioned embodiment of the present disclosure. Figure 8 As shown, the microfluidic chip 300 further includes a sealing structure mounting portion 304. For example, the sealing portion 201 of the sealing structure can be mounted on the sampling structure mounting portion 303, and the fixing portion 202 of the sealing structure can be mounted on the sealing structure mounting portion 304.

[0082] For example, in some embodiments, see Figure 4 In the case where the fixing structure of the sealing structure is an annular sleeve structure, the sealing structure mounting portion 304 includes an annular groove 3041 that matches the annular sleeve structure. Thus, the sealing structure can be stably fixed to the microfluidic chip.

[0083] For example, in some embodiments, see Figure 4In the case where the sealing portion 201 of the sealing structure includes a protrusion 2013 for sealing, the sampling structure mounting portion 303 includes a slot 3031 that matches the protrusion 2013. Thus, the sealing portion 201 can simultaneously achieve the fixing function and the sealing function. In addition, due to the inclination angle of the side wall of the protrusion 2013, when the protrusion 2013 is pressed into the slot 3031, the two can further improve the sealing effect by contacting each other.

[0084] For example, in some embodiments, see Figure 3 The detection assembly further includes a push rod 2014, which is movably arranged, for example, driven by a driving device (such as a stepping motor), so that it can move in the up and down directions in the figure, and the push rod 2014 can be used to apply a force to the main body 2011 of the sealing part 201. For example, the force applied by the push rod 2014 to the main body 2011 can be adjusted, so that the purpose of adjusting the internal pressure of the sampling structure can be achieved by controlling the push rod 2014.

[0085] For example, in some embodiments, the main body 2011 of the sealing part 201 further includes a concave groove 2015 that cooperates with the push rod 2014 to guide the force application position of the push rod 2014. For example, the concave groove 2015 has the same shape as the push rod 2014, for example, their cross sections are both circular. For example, the diameter of the concave groove 2015 is slightly larger than the diameter of the push rod 2014, and has a step, so as to facilitate guiding the force application position of the push rod 2014.

[0086] Next, combine Fig.10 and Fig.11 The working process of the detection component is introduced exemplarily.

[0087] First, the sample to be detected is sucked by the sampling structure. At this time, the sample to be detected can be sucked from the first channel and the second channel of the sampling structure by capillary action. The sample to be detected can be, for example, blood, body fluid, etc., which is not limited in the embodiments of the present disclosure.

[0088] Then, the sampling structure is installed on the microfluidic chip, and the packaging structure is used to fix and seal the sampling structure. For example, after the sampling structure is installed on the microfluidic chip, the bottom of the sampling structure extends into the reagent pool of the microfluidic chip so that the sample to be detected can be mixed with the detection reagent in the reagent pool. For example, the upper surface of the reagent pool has an aluminum foil film for encapsulating the detection reagent. When the sampling structure is installed on the microfluidic chip, the sampling structure can pierce the aluminum foil film to communicate with the reagent pool. For example, the bottom of the reagent pool is heat-sealed with an aluminum foil film. After the sampling structure and the sealing structure are installed, two communicating sealed cavities are formed in the microfluidic chip, one is the first sealed cavity formed by the first channel, the second channel and the chamber in the sampling structure, and the other is the second sealed cavity formed by the reagent pool.

[0089] like Fig.10 As shown, a push rod 2014 is used to apply a force to the main body 2011 of the sealing part. When the force is weak and less than the threshold pressure, the exhaust hole is in an open state, and the sampling structure can discharge air or overflow through the exhaust hole. When the applied force gradually increases to be greater than or equal to the threshold pressure, the exhaust hole is in a closed state. At this time, the pressure inside the sampling structure increases, so that the sample to be tested is pushed out of the sampling structure and enters the reagent pool. Fig.10 As shown by the arrow in FIG. , the sample and the detection reagent in the reagent pool can be mixed.

[0090] like Fig.11 As shown, when the push rod 2014 is withdrawn, the main body 2011 rebounds, and at this time, the mixed liquid of the detection reagent and the sample to be detected in the reagent pool will be sucked back into the sampling structure. Since in the sampling structure, there is a partition column in the second channel, and there are multiple narrow gaps between adjacent partition columns and between the partition columns and the channel wall, the flow rate of the mixed liquid will be accelerated after passing through the first channel and the second channel, and then the mixed liquid can rush into the cavity of the sampling structure at a relatively high speed, and form a swirling mixture with the sample to be detected in the sampling structure, as shown in FIG. Fig.11 As shown by the arrow in , the mixing efficiency can be improved, and the detection reagent and the sample to be detected can be mixed more evenly. At this point, a mixing operation is completed.

[0091] For example, the above mixing operation can be performed multiple times to further improve the mixing effect of the sample to be detected and the detection reagent.

[0092] For example, after the detection reagent and the sample to be detected are fully mixed, the reagent cell can inject the mixed solution into the detection component through the microchannel connected thereto. In this process, a force can be applied to the main body 2011 through the push rod 2014 so that the mixed solution can enter the microchannel. At this time, since the main body 2011 has an exhaust hole, the pressure in the sampling structure and the reagent cell will not increase rapidly during the force application process, thereby preventing the mixed solution from rapidly pouring into the microchannel in a short time, and further more accurately controlling the output speed and output amount of the mixed solution.

[0093] When the mixed liquid enters the detection component, the detection component can detect the sample, thereby realizing the automated detection process of the microfluidic chip.

[0094] It should be noted that, in some embodiments, the sealing structure matched with the sampling structure may also have only a sealing portion without a fixing portion, see Figure 6 and Figure 7 At this time, the sealing structure can also realize the functions of sealing and fixing the sampling structure.

[0095] For example, in some embodiments, the microfluidic chip may further include a mixing structure and a filtering structure connected to the middle of the microchannel, and the embodiments of the present disclosure do not limit other functional structures of the microfluidic chip. For example, the mixing structure can further improve the mixing effect of the detection reagent and the sample to be detected. The filtering structure can filter the mixture of the detection reagent and the sample to be detected to obtain a pure sample to be detected for detection. For example, when the sample to be detected is blood, the filtering structure can filter out blood clots and other substances that may exist in the blood and affect the detection.

[0096] The detection component provided by at least one embodiment of the present disclosure can complete the analysis and detection process of the sample to be detected through simple operations after the sampling of the sample to be detected is completed, and the analysis and detection result is more accurate.

[0097] There are a few points to note:

[0098] (1) The drawings of the embodiments of the present disclosure only relate to the structures related to the embodiments of the present disclosure, and other structures may refer to the general design.

[0099] (2) For the sake of clarity, in the drawings used to describe the embodiments of the present disclosure, the thickness of layers or regions is enlarged or reduced, that is, these drawings are not drawn according to the actual scale.

[0100] (3) In the absence of conflict, the embodiments of the present disclosure and the features therein may be combined with each other to obtain new embodiments.

[0101] The above is only a specific embodiment of the present disclosure, but the protection scope of the present disclosure is not limited thereto. Any person skilled in the art who is familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed in the present disclosure, which should be included in the protection scope of the present disclosure. Therefore, the protection scope of the present disclosure should be based on the protection scope of the claims.

Claims

1. A sampling structure, comprising: A first body including a first channel, wherein the first channel includes an exposed first opening; A second body connected to the first body, comprising a second channel and at least one partition column located in the second channel, wherein the second channel is connected to the first channel, and a first gap is formed between the partition column and a channel wall of the second channel; a third body connected to the second body, comprising a chamber, wherein the chamber is communicated with the second channel and can accommodate a sample; The at least one partition column comprises a plurality of partition columns, the first channel comprises a second opening connected to the second channel, the plurality of partition columns are arranged around the second opening at intervals, and the extension direction of the plurality of partition columns is parallel to the central axis of the second opening; The plurality of partition columns are centrally symmetrically arranged around the central axis of the second opening, each of the partition columns at least partially overlaps with the second opening, and a second gap is provided between adjacent partition columns.

2. The sampling structure according to claim 1, wherein: The cross section of each of the partition columns is fan-shaped.

3. The sampling structure according to claim 2, wherein: The plurality of partition columns are arranged relative to each other and the resulting arrangement is cylindrical in shape as a whole.

4. The sampling structure according to any one of claims 1 to 3, wherein: The second gap width is 0.1 mm-2.5 mm.

5. The sampling structure according to claim 4, wherein: The radius of the first channel is 0.1 mm-3 mm, and the radius of the second channel is 1 mm-10 mm.

6. The sampling structure according to claim 5, wherein: The cross section of each of the partition columns is fan-shaped, and the radius of the fan-shaped fan is 0.5 mm-8 mm.

7. The sampling structure according to any one of claims 1 to 3, wherein: The length of the first channel is 0.1 mm-10 mm, and the length of the second channel is 1 mm-10 mm.

8. The sampling structure according to any one of claims 1 to 3, wherein: The first body, the second body and the third body are an integrated structure.

9. A sealing structure comprising: A sealing part, comprising a main body part having elasticity; Wherein, the sealing portion comprises at least one exhaust hole, and the exhaust hole is configured to be in an open state or a closed state respectively under different forces acting on the main body; When the force applied to the main body is less than a threshold value, the exhaust hole is in the open state; when the force applied to the main body is greater than or equal to the threshold value, the exhaust hole is in the closed state; The exhaust hole is a triangular prism exhaust hole, including a first wall and a second wall connected to the main body. The first wall and the second wall are connected, and two opposite triangular openings are formed between the first wall, the second wall and the main body. When the force applied to the main body is greater than or equal to the threshold, the first wall and the second wall may be stretched to be located in the same plane, so that the triangular opening is closed and the exhaust hole is in the closed state; The sealing portion further includes a protrusion for sealing.

10. The sealing structure according to claim 9, further comprising a fixing portion, The fixing portion is fixedly connected to the sealing portion and includes a fixing structure.

11. The sealing structure according to claim 10, wherein: The fixing structure is an annular sleeve structure.

12. The sealing structure according to claim 9, wherein: The raised portion is arranged around the main body portion, The longitudinal section of the protrusion is in a shape that gradually narrows from the first end to the second end, wherein the first end is an end connected to the main body, and the second end is an end opposite to the first end.

13. The sealing structure according to claim 10 or 11, wherein: The fixing portion and the sealing portion are an integrated structure.

14. A detection component comprising: A microfluidic chip, a sampling structure and a sealing structure according to any one of claims 1 to 8, The microfluidic chip comprises a sampling slot and a sampling structure mounting portion, wherein the sampling structure mounting portion is in communication with the sampling slot. The sampling structure may be disposed in the sampling slot, and the sealing structure may be installed in the sampling structure installation portion to seal the sampling structure.

15. The detection assembly according to claim 14, wherein: The sealing structure is the sealing structure according to any one of claims 10, 11 and 13, and the microfluidic chip further comprises a sealing structure mounting portion, Wherein, the sealing part of the sealing structure can be installed on the sampling structure installation part, and the fixing part of the sealing structure can be installed on the sealing structure installation part.

16. The detection assembly according to claim 15, wherein: The fixing structure of the sealing structure is an annular sleeve structure, and the sealing structure mounting portion includes an annular groove matched with the annular sleeve structure.

17. The detection assembly according to claim 15, wherein: The sealing portion of the sealing structure further comprises a protruding portion for sealing, and the sampling structure mounting portion comprises a slot matched with the protruding portion.

18. The detection assembly according to claim 15, further comprising a push rod, wherein: The push rod is movably arranged to apply a force to the main body.

19. The detection assembly according to claim 18, wherein: The main body also includes a concave groove matched with the push rod to guide the force application position of the push rod.

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