A traditional Chinese medicine hair-blackening composition and preparation thereof

Abstract

The present invention discloses a hair-blackening Chinese herbal medicine composition and a corresponding preparation method. The composition primarily contains the following ingredients: Ligustrum lucidum fruit, Polygonum multiflorum, dried ginger, Salvia miltiorrhiza, Taraxacum officinale, and Perilla leaf. This combination, through a specific extraction process, yields an extract with excellent antibacterial and anti-inflammatory properties. This effect is achieved by increasing tyrosinase activity in melanocytes in hair follicles, thereby increasing melanin production within the follicles.

Description

Technical Field

[0001] The invention relates to the fields of cosmetics and medicines, and particularly to a traditional Chinese medicine hair-blackening composition and a preparation thereof. Background Art

[0002] According to extensive research, the main cause of gray hair, besides genetics, is nutritional imbalance or mental overstimulation, leading to a lack of adequate nourishment for the hair. This condition is particularly pronounced in adolescents and middle-aged adults, who often experience gray hair, which can make them appear older and even affect their employment prospects. The age, range, and degree of gray hair onset vary significantly between individuals and may be influenced by genetics, nutrition, mental health, and environmental factors. Statistics show that 25% of people experience scattered gray hair around the age of 30, and 50% around the age of 40.

[0003] Younger individuals with gray hair often seek methods to darken their hair, including medication to regulate endocrine function, hair dyeing, and other massage and physiological treatments, but these methods often yield unsatisfactory results. Hair dyes often contain carcinogens like xylene and can cause skin allergies. Due to the side effects of medication for endocrine regulation and hair dyeing, modern beauty, hairdressing, and medical fields have also begun to explore the therapeutic benefits of Traditional Chinese Medicine (TCM), particularly topical hair growth products. While some TCM hair growth products currently on the market have some effectiveness, the effects are often limited and the treatment time is relatively long. Therefore, most individuals with gray hair still prefer to dye their hair.

[0004] Currently, there are few Western medicine treatments for gray hair. Literature reports suggest that adenosine cyclic monophosphate can promote protein and RNA synthesis, potentially delaying the onset of gray hair. Methoxsalen, which stimulates tyrosinase activity, can also help darken gray hair to a certain extent. Traditional Chinese medicine treatments for gray hair are plentiful, but they are often ineffective, expensive, and require lengthy treatments.

[0005] Ligustrum lucidum fruit is the fruit of the Ligustrum lucidum plant, a member of the Oleaceae family. It is neutral in nature, sweet and bitter in flavor, and enters the liver and kidney meridians. It has the benefits of nourishing yin and promoting longevity, strengthening the liver and kidneys, clearing away heat and improving eyesight, and promoting hair growth. In Traditional Chinese Medicine, it is commonly used to treat symptoms such as dizziness, tinnitus, soreness of the waist and knees, and premature graying of hair caused by internal heat.

[0006] Polygonum multiflorum, also known as Polygonum multiflorum, Purple Polygonum multiflorum, and Night-crossing Vine, is a perennial twining vine of the Polygonaceae family. Its tuberous roots are used as medicine to soothe the mind and nourish the brain. Processed Polygonum multiflorum can replenish essence and blood, blacken hair, strengthen shins, and nourish the liver and kidneys. It is the preferred herb for treating gray hair.

[0007] Dried ginger, the dried rhizome of the ginger plant (Zingiber officinale), can draw blood-boosting herbs into the blood and qi-boosting herbs into the qi. It also removes negative energy and nourishes new energy, promoting the growth of yang and yin, making it suitable for those with blood deficiency. Gingerol, a compound found in ginger, promotes capillary dilation and invigorates blood circulation.

[0008] Salvia miltiorrhiza is bitter in nature and slightly cold in nature. It is mainly used in traditional Chinese medicine to remove blood stasis and relieve pain, promote blood circulation and dredge menstruation, clear the heart and relieve restlessness. It is used for symptoms such as irregular menstruation, abdominal pain and swelling, restlessness and insomnia.

[0009] Dandelion, belonging to the Asteraceae family, is a perennial herb with sweet, bitter and cold properties. It enters the liver and stomach meridians and has diuretic, laxative, jaundice-relieving and choleretic effects. It can treat heat toxins and has a good effect on colds and fevers.

[0010] Perilla leaf, belonging to the Lamiaceae family, is a warm annual herb that can dispel wind, dispel cold, and remove dampness. It is suitable for summer colds and can also detoxify, prevent and relieve seafood poisoning. The perilla leaf flavonoids have good antibacterial and antimicrobial effects.

[0011] Chinese patent CN106581456A discloses a traditional Chinese medicine composition that can turn white hair into black hair and its preparation method. The composition includes Chinese herbal medicines such as Rehmannia glutinosa, Ligustrum lucidum fruit, Salvia miltiorrhiza, Pegasus magnolia, mulberry, chrysanthemum, Chuanxiong, rose, Eucommia ulmoides, Polygonum multiflorum, Salvia miltiorrhiza, Panax notoginseng, Cistanche deserticola, black sesame, and black beans. It needs to be soaked in a large amount of white wine and mineral water and needs to be sealed for several months. The preparation method is cumbersome and the proportion of medicinal materials is complex. Therefore, it is of great significance to find a black hair compound with a more reasonable prescription, simpler preparation process, easily available materials, easy use, significant efficacy and no rebound. Summary of the Invention

[0012] The present invention addresses the shortcomings of existing medications and aims to provide a Chinese herbal compound hair-blackening composition with readily available raw materials, significant efficacy, and ease of use. Through extensive in vivo and in vitro screening, the inventors discovered a novel hair-blackening composition with excellent synergistic effects, superior hair-blackening efficacy, and no toxic side effects.

[0013] To achieve the above object, the present invention provides the following technical solutions:

[0014] A traditional Chinese medicine composition for black hair consists of the following components in parts by weight: 0-30 parts of glossy privet fruit, 0-30 parts of polygonum multiflorum, 0-30 parts of dried ginger, 0-30 parts of salvia miltiorrhiza, 0-10 parts of dandelion, and 0-30 parts of perilla leaf.

[0015] Preferably, 20-30 parts of Ligustrum lucidum fruits, 0-20 parts of Polygonum multiflorum, 10-30 parts of dried ginger, 0-30 parts of Salvia miltiorrhiza, 0-10 parts of Taraxacum officinale, and 0-30 parts of Perilla leaves are used.

[0016] More preferably, 20 parts of Ligustrum lucidum fruits, 20 parts of Polygonum multiflorum, 10 parts of dried ginger, 20 parts of Salvia miltiorrhiza, 10 parts of Taraxacum mongolicum, and 20 parts of Perilla frutescens leaves.

[0017] The hair-blackening composition of the present invention has an extract preparation method as follows: weigh the powders of Chinese medicinal materials such as Ligustrum lucidum, Polygonum multiflorum, dried ginger, Salvia miltiorrhiza, and Taraxacum mongolicum by weight, add them to an ethanol solution for soaking, heat and reflux for extraction, filter, and collect the filtrate; then weigh the powder of Perilla frutescens leaves by weight, add them to water for soaking, heat and reflux for extraction, filter, and collect the filtrate; then combine the powders with the aforementioned ethanol extract, and concentrate under reduced pressure to obtain a paste.

[0018] Preferably, according to the above-mentioned Chinese medicinal materials by weight, weigh the powder of Chinese medicinal materials such as Ligustrum lucidum, Polygonum multiflorum, dried ginger, Salvia miltiorrhiza, and dandelion, add 10 times the amount of 60% ethanol, soak overnight, heat under reflux at 75°C for extraction for 2h, filter, collect the filter residue, extract again under the same conditions and filter, and combine the two extracts; weigh the prescribed amount of Perilla leaf powder, add 10 times the amount of deionized water, soak overnight, heat under reflux at 90°C for extraction for 2h, filter, collect the filter residue, extract again under the same conditions and filter, combine the two extracts, and then combine with the aforementioned 60% ethanol extract, and concentrate under reduced pressure at 55°C to a paste.

[0019] The present invention also provides a hair-blackening hydrogel, which is prepared by the following method: ①. Taking 2%-3% carbomer 940 as a base, take 4 parts and add 15 parts of glycerin to obtain A. ②. Weighing approximately 2 parts of the hair-blackening composition, adding 1.5 parts of a 60-95% ethanol solution, stirring to fully dissolve and mix uniformly, to obtain B. ③. Slowly adding the uniformly mixed B to A while stirring to fully mix, to obtain C. ④. Adding 0.2 parts of azone and 0.5 parts of essence to C, stirring to fully mix, to obtain D. E. Adding triethanolamine to D while stirring to obtain a viscosity of 3.5±0.5 Pa·s, to obtain a topical hair-blackening hydrogel.

[0020] The hair-blackening composition of the present invention can be prepared into common hair-blackening cosmetics, medicinal cosmetics, or traditional Chinese medicine external preparations.

[0021] The medicinal materials described in the present invention include corresponding raw materials and decoction pieces thereof.

[0022] From the perspective of improving the scalp environment and activating melanocyte tyrosinase, the present invention combines active ingredients such as flavonoids, polysaccharides, organic acids in Chinese herbal medicine to conduct preliminary screening of Chinese herbal medicine compounds. At the same time, a mouse white hair model is established, and the hair blackening effect of the combined compound is studied through in vivo animal experiments. It is found that the combination of Ligustrum lucidum fruit, Polygonum multiflorum, dried ginger, Salvia miltiorrhiza, dandelion, and Perilla leaf has a good hair blackening effect, and is innovative and practical.

[0023] The positive effects of the present invention are: establishing a tyrosinase screening model and an animal gray hair model, combining in vivo and in vitro experiments for screening, and obtaining a Chinese herbal medicine composition with good hair blackening effect. The preparation process is simple, easy to use, no chemical drugs are added, it has a good hair blackening effect, is safe and has no toxic side effects, and can be used for a long time. DETAILED DESCRIPTION

[0024] Example 1

[0025] In vitro experiments: 18 Chinese medicinal materials including Eclipta prostrata, Houttuynia cordata, Scutellaria baicalensis, Platycladus orientalis leaves, Astragalus membranaceus, Angelica dahurica, Ligustrum lucidum fruit, Polygonum multiflorum, dried ginger, Salvia miltiorrhiza, Taraxacum mongolicum, Perilla leaves, Sophora flavescens, Glycyrrhiza uralensis, loquat leaves, and Ligusticum chuanxiong were extracted with water, 60% ethanol, or 95% ethanol. The extracts were screened using the DOPA rate oxidation test to obtain the medicinal extracts with a strong tyrosinase activation effect. The medicinal materials were then combined to screen the enzyme activity.

[0026] DOPA rate oxidation experimental method:

[0027] Dissolve 1.0 mg (1380 IU) of tyrosinase in 25 ml of PBS (0.05 mol / L, pH 6.8) to a concentration of 55 IU / ml. Prepare a 0.15% solution of L-Dopa in the same PBS. Add the following reagents to the 250 μl reaction mixture:

[0028] Table 1 Addition volume (unit: microliter)

[0029]

[0030] The experiment was conducted in a 96-well plate. Dopa was added after a 10-minute reaction at 37°C. The absorbance was immediately measured at a wavelength of 490 nm 2 minutes later. Three parallel wells were set up for each group. The enzyme activation rate was calculated according to the following formula: Tyrosinase activation rate (%) = [(CD) - (AB)] / (AB) * 100

[0031] Wherein, A: absorbance measured by the enzyme mixture without adding Chinese medicine sample;

[0032] B: absorbance measured by the mixture without adding Chinese medicine sample or enzyme;

[0033] C: absorbance measured by adding the mixture of Chinese medicine sample and enzyme;

[0034] D: Absorbance measured by the mixture of Chinese medicine sample without enzyme; A and B are the controls of each group

[0035] Table 2: Enzyme activation rates of various combinations and single extracts

[0036]

[0037]

[0038] Through screening and combination, the combinations with stronger tyrosinase activation effect were obtained, namely, Ligustrum lucidum fruit + Polygonum multiflorum + Salvia miltiorrhiza, Ligustrum lucidum fruit + Polygonum multiflorum + Perilla leaf, and Polygonum multiflorum + Salvia miltiorrhiza + Perilla leaf. The activation rates were significantly higher than those of each monomer enzyme, and also higher than those of the combination of two of them, showing a strong synergistic effect. Due to the limitation of the accuracy of the enzyme screening experiment, only the combination of three herbs can be carried out, and subsequent screening will be carried out through animal experiments.

[0039] Example 2

[0040] In vivo experiments: After in vitro enzyme activity screening, three groups of herbal combinations with synergistic effects were obtained. Each group has three herbs. After a large number of preliminary experiments and preliminary efficacy evaluations, it was found that adding other herbs to these three combinations would increase the hair-blackening effect. Dried ginger and dandelion had the most significant effect. Finally, 6 groups of compound prescriptions were designed for in vivo animal testing. The specific prescriptions are shown in the table below:

[0041] Table 3 Ratios of medicinal materials in the six experimental groups

[0042] serial number Ligustrum lucidum Polygonum multiflorum dried ginger Salvia miltiorrhiza dandelion Perilla leaves (water extraction) 1 0 30 0 30 10 30 2 30 0 30 10 0 30 3 10 30 0 30 0 30 4 20 20 10 20 10 20 5 20 20 20 0 10 30 6 20 20 10 30 10 0

[0043] Experimental methods:

[0044] 1) Extraction of Chinese medicinal materials: Ligustrum lucidum fruit, Polygonum multiflorum, Salvia miltiorrhiza, Taraxacum mongolicum, and dried ginger were weighed according to the ratio in the table, 10 times the amount of 60% ethanol was added, soaked for 12 hours, refluxed at 75°C for 2 hours, extracted twice, combined the extracts, and filtered; Perilla leaf was extracted alone with 10 times the amount of deionized water, refluxed at 90°C, extracted twice, each time for 2 hours, combined the extracts, filtered, combined with the previous medicinal material extract, and concentrated under reduced pressure at 55°C to a paste to obtain each combined extract 1-6.

[0045] 2) Preparation of Hair Blackening Hydrogel: ①. Take 4 parts of 2% Carbomer 940 as the base and add 15 parts of glycerin to obtain A. ②. Weigh approximately 2 parts of the extract from the above composition and add 1.5 parts of 60% ethanol solution. Stir to thoroughly dissolve and mix to obtain B. ③. Slowly add the thoroughly mixed B to A while stirring to obtain C. ④. Add 0.2 parts of azone and 0.5 parts of fragrance to C and stir to obtain D. E. Add triethanolamine to D while stirring to obtain a viscosity of 3.5±0.5 Pa·s to obtain a topical hair blackening hydrogel. Numbers 1-6 correspond to the extracts in the corresponding groups.

[0046] Example 3: Investigation of Hair-Blackening Effect

[0047] Experimental animals: C57 mice, male, weighing 18-20 g, SPF grade, provided by Shanghai Xipu-Bikai Animal Laboratory Co., Ltd., Animal Production License No.: SCXK (Shanghai) 2013-0016

[0048] Establishment of a hydroquinone-induced gray hair mouse model:

[0049] On the first day of the experiment, mice were anesthetized with 4% chloral hydrate via intraperitoneal injection. The mice were weighed and injected at a rate of 0.1 ml / 10 g body weight. After anesthesia, the hair on the backs of the mice was shaved with an electric razor, being gentle to avoid damaging the skin. Then, 1 ml of 6% sodium sulfide was evenly applied to the backs of the mice using a 1 ml syringe. After 2.5 minutes, the hair on the backs of the mice was completely shed, leaving the skin intact.

[0050] Starting on the seventh day of the experiment, use a 1ml syringe to draw up hydroquinone and apply 0.5ml to the outer part of each mouse's hair removal area, completely soaking it. Leave it on for 5 minutes before returning the mouse to its cage. Apply the solution twice daily, at 9:00 and 16:00. Thirty days after modeling, the model is complete when white hair on the mouse's back covers at least 80% of the experimental area.

[0051] Grouping and Dosing: The successfully modeled mice were divided into seven groups of eight mice each, designated the control group, which received blank gel. The other six groups, designated the observation group, received 0.2 g of the hydrogels prepared in Example 2 (Groups 1-6) twice daily. The mice were observed for changes in hair color from white to black. Administration was gentle and continued for at least 5 minutes before returning the mice to their cages to allow for full absorption of the drug.

[0052] Testing indicators: After the experiment, the improvement effect of each experimental group was observed, and the 2*2cm2 area in the center of the medication site was selected as an observation unit to determine the effect. The standards are as follows: "5" means that the newly grown hair in the observation area is black >75%; "4" means that the newly grown black hair in the observation area is black >50%; "3" means that the newly grown black hair in the observation area is black >25%; "2" means that the black hair in the observation area is less than 25%; "1" means that the hair in the observation area is completely white or there are scattered black hairs.

[0053] The scoring criteria are as follows:

[0054]

[0055]

[0056] Experimental results: see Table 4

[0057] Table 4 Effect of Chinese medicine composition hydrogel on blackening of white hair in mice (n=6, x±SD)

[0058] serial number Group Black hair score (out of 5 points) 1 control group 2.32±0.61 2 Product 1 group 4.02±0.42 3 Product 2 Group 4.12±0.87 4 Product 3 groups 3.85±0.69 5 Product 4 groups 4.76±0.77 6 Product 5 groups 4.21±0.95 7 6 groups of products 4.33±0.67

[0059] The results showed that when comparing the hair blackening of mice in each group, the score of mice in the model group was significantly lower than that of mice in each Chinese medicine group, indicating that the model was successfully established and each combination showed different degrees of hair blackening effect.

Claims

1. A Chinese medicine composition for black hair, consisting of the following components in parts by weight: 30 parts of Polygonum multiflorum, 30 parts of Salvia miltiorrhiza, 10 parts of Taraxacum herb, 30 parts of Perilla frutescens leaves; or, The invention is composed of the following components in parts by weight: 30 parts of Ligustrum lucidum fruit, 30 parts of dried ginger, 10 parts of Salvia miltiorrhiza and 30 parts of Perilla leaf; or, According to weight, it is composed of the following components: 10 parts of Ligustrum lucidum fruit, 30 parts of Polygonum multiflorum, 30 parts of Salvia miltiorrhiza, and 30 parts of Perilla leaf; or, The invention is composed of the following components in parts by weight: 20 parts of Ligustrum lucidum fruit, 20 parts of Polygonum multiflorum, 10 parts of dried ginger, 20 parts of Salvia miltiorrhiza, 10 parts of Taraxacum officinale, and 20 parts of Perilla leaf; or, The invention is composed of the following components in parts by weight: 20 parts of Ligustrum lucidum fruit, 20 parts of Polygonum multiflorum, 20 parts of dried ginger, 10 parts of dandelion, and 30 parts of Perilla leaf; or, The invention is composed of the following components in parts by weight: 20 parts of Ligustrum lucidum fruits, 20 parts of Polygonum multiflorum, 10 parts of dried ginger, 30 parts of Salvia miltiorrhiza and 10 parts of Taraxacum mongolicum.

2. The Chinese medicine composition according to claim 1, wherein the Chinese medicine composition is prepared by the following method: weighing the powder of the Chinese medicinal materials Ligustrum lucidum, Polygonum multiflorum, dried ginger, Salvia miltiorrhiza, and Taraxacum mongolicum in parts by weight, soaking them in an ethanol solution, heating and refluxing for extraction, filtering, and collecting the filtrate; then weighing the powder of the Perilla leaf in parts by weight, soaking them in water, heating and refluxing for extraction, filtering, and collecting the filtrate; and then combining the powder with the aforementioned ethanol extract and concentrating under reduced pressure to a paste.

3. The Chinese medicine composition according to claim 2, wherein the Chinese medicine composition is prepared by the following method: weighing the powder of the Chinese medicinal materials of Ligustrum lucidum, Polygonum multiflorum, dried ginger, Salvia miltiorrhiza, and Taraxacum mongolicum in parts by weight, adding 10 times the amount of 60% ethanol and soaking overnight, heating and reflux extraction at 75°C for 2 hours, filtering, collecting the filter residue, extracting again under the same conditions and filtering, and combining the two extracts; weighing the powder of Perilla frutescens leaf in parts by weight, adding 10 times the amount of deionized water and soaking overnight, heating and reflux extraction at 90°C for 2 hours, filtering, collecting the filter residue, extracting again under the same conditions and filtering, combining the two extracts, and then combining with the aforementioned 60% ethanol extract, and concentrating under reduced pressure at 55°C to a paste.

4. Use of the Chinese medicine composition for black hair as described in any one of claims 1 to 3 in the preparation of common cosmetics, medicinal cosmetics, or Chinese medicine external preparations.

5. A hair-blackening hydrogel is prepared by the following method: ① Take 4 parts of 2% carbomer 940 as the matrix, add 15 parts of glycerol to obtain A; ② Weigh 2 parts of the traditional Chinese medicine composition for hair-blackening according to any one of claims 1 to 4, add 1.5 parts of 60% ethanol solution, stir to fully dissolve and mix evenly, and obtain B; ③ Slowly add the evenly mixed B to A, stirring while adding to mix evenly, and obtain C; ④ Add 0.2 parts of azone and 0.5 parts of essence to C, stir and mix evenly, and obtain D; E. Add triethanolamine to D while stirring to make its viscosity 3.5±0.5 Pa·s, and obtain an external hair-blackening hydrogel.

6. Use of the black hair hydrogel according to claim 5 in the preparation of common cosmetics, medicinal cosmetics or traditional Chinese medicine external preparations.

Images