Vaccination device, vaccination system and vaccination method

Abstract

The present invention provides an inoculation device, an inoculation system and an inoculation method, which relate to the technical field of inoculation devices, and solve the technical problem of the uneven size of wounds caused by the inoculation device on the stem of a plant in the study of the pathogenicity of plant pathogens to a certain extent. The inoculation device provided by the present invention comprises a first blade, a second blade and an expansion mechanism; the first blade and the second blade are arranged relative to each other along a first direction, a receiving cavity is formed between the first blade and the second blade, and the first end of the first blade and the first end of the second blade jointly form the inlet end of the receiving cavity; the first end to the second end of the first blade and the second blade are both gradually contracted, and the second end of the first blade and the second end of the second blade fit together to form a blade; the expansion mechanism can enter the receiving cavity through the inlet end and move in the direction close to the blade, so that the second end of the first blade and the second end of the second blade move relative to each other in directions away from each other to achieve the expansion of the blade.

Description

Technical Field

[0001] The present invention relates to the technical field of vaccination devices, and in particular to a vaccination device, a vaccination system and a vaccination method. Background Art

[0002] In the process of carrying out plant inoculation to induce disease, the previous technical means was to use a knife or scissors to puncture the plant stem to create a wound, and then inoculate the fungi cultured on the culture medium or other carriers by hand, knife or scissors.

[0003] This method will not only cause problems such as inconsistent wound size, inconsistent inoculation depth, and inconsistent inoculation amount, but also the use of inoculation methods such as hands, knives, or scissors will contaminate the wound, resulting in uneven disease in subsequent plants, and thus causing unusable experimental research data or experimental failure.

[0004] Therefore, there is an urgent need to provide an inoculation device, an inoculation system and an inoculation method to solve the problems existing in the prior art to a certain extent. Summary of the invention

[0005] The object of the present invention is to provide an inoculation device, an inoculation system and an inoculation method, so as to solve the technical problem of the uneven size of wounds caused by the inoculation device on the plant stem in the study of the pathogenicity of plant pathogens to a certain extent.

[0006] The present invention provides an inoculation device, comprising a first blade body, a second blade body and an expansion mechanism; the first blade body and the second blade body are arranged relative to each other along a first direction, a receiving cavity is formed between the first blade body and the second blade body, and the first end of the first blade body and the first end of the second blade body jointly form an inlet end of the receiving cavity; the first end to the second end of the first blade body and the first end to the second end of the second blade body are both gradually contracted, and the second end of the first blade body and the second end of the second blade body are fitted together to form a blade portion; the expansion mechanism can enter the receiving cavity through the inlet end and move in a direction close to the blade portion, so that the second end of the first blade body and the second end of the second blade body move relative to each other in directions away from each other, so as to achieve expansion of the blade portion.

[0007] It also includes a first limiting mechanism, which is arranged along a first direction on a side of the first blade body and / or the second blade body away from the accommodating cavity, and one end of the first limiting mechanism facing the blade is a limiting end, and there is a predetermined distance between the limiting end and the blade, so that when the blade penetrates into a predetermined depth in the plant stem, one end of the first limiting mechanism can abut against the outer wall of the plant stem.

[0008] Specifically, the length of the first blade body in the first direction is greater than the length of the second blade body, and along the first direction, the first end of the first blade body and the first end of the second blade body are flush, and the second end of the first blade body protrudes beyond the second end of the second blade body; the first limiting mechanism includes a first positioning seat and a first limiting rod; the first positioning seat is arranged on the first blade body, the first limiting rod is arranged along the first direction, and is movably connected to the first positioning seat, so that one end of the first limiting rod can move toward or away from the blade portion; the first limiting rod forms a limiting end toward one end of the blade portion, and the end face of the limiting end is an arc surface.

[0009] Wherein, the expansion mechanism includes an expansion component and a first push rod; the size of the expansion component matches the size of the inlet end of the accommodating cavity, the expansion mechanism includes two expansion surfaces arranged back to back and with a certain distance, and the two expansion surfaces are respectively abutted against the inner wall surfaces of the first blade body and the second blade body; the first push rod is used to push the expansion mechanism in the direction close to the blade portion.

[0010] Specifically, the expansion member includes a first expansion piece, a second expansion piece and a connecting rod; the first expansion piece is correspondingly fitted with the first blade body, the second expansion piece is correspondingly fitted with the second blade body, a groove portion is formed on the connecting rod, and the first push rod can be inserted into the groove portion to push the expansion member; a connecting hole is opened in the groove portion along the first direction, and a connecting portion is formed at one end of the first push rod facing the groove portion, and the connecting portion can be inserted into the connecting hole to connect the first push rod to the expansion member.

[0011] Furthermore, the inoculation device provided by the present invention also includes a second limiting mechanism, which includes a connecting block, a second positioning seat and a second limiting rod; one end of the connecting block is connected to the connecting rod, and the other end extends out of the accommodating cavity; the second positioning seat is arranged at one end of the connecting block extending out of the accommodating cavity, and the second limiting rod is movably connected to the second positioning seat along the first direction; the second limiting rod can follow the expansion member to move in the direction close to the blade, and one end of the second limiting rod can abut against the outer wall of the plant stem to limit the depth of the expansion member entering the plant stem.

[0012] Among them, the vaccination device provided by the present invention also includes a positioning block and a handle; the positioning block is arranged along the second direction, and the first end of the first blade body and the first end of the second blade body are both connected to the positioning block; the end of the positioning block away from the first blade body and the second blade body is connected to the handle.

[0013] Compared with the prior art, the inoculation device provided by the present invention has the following advantages:

[0014] The inoculation device provided by the present invention comprises a first blade body, a second blade body and an expansion mechanism; the first blade body and the second blade body are arranged opposite to each other along a first direction, a receiving cavity is formed between the first blade body and the second blade body, and the first end of the first blade body and the first end of the second blade body jointly form an inlet end of the receiving cavity; the first end to the second end of the first blade body and the first end to the second end of the second blade body are both gradually contracted, and the second end of the first blade body and the second end of the second blade body are fitted together to form a blade portion; the expansion mechanism can enter the receiving cavity through the inlet end and move in a direction close to the blade portion, so that the second end of the first blade body and the second end of the second blade body move relative to each other in directions away from each other, so as to achieve expansion of the blade portion.

[0015] From this analysis, it can be seen that the blade formed by the first blade body and the second blade body can penetrate into the plant stem to form an inoculation wound. The accommodating cavity formed by the first blade body and the second blade body arranged opposite to each other can enable the expansion mechanism to move from the inlet end to the direction close to the blade formed by the first blade body and the second blade body, so that the first blade body and the second blade body can expand in a direction away from each other, thereby expanding the inoculation wound.

[0016] Without changing the size of the expansion mechanism, the expansion size of the inoculation wound caused by the expansion mechanism can be kept consistent to a certain extent, so that the size of the inoculation cavity formed by each plant stem can be kept consistent to a certain extent, thereby achieving the unification of wound size and inoculation depth, and improving the accuracy of the test.

[0017] In addition, the present invention also provides an inoculation system, including the above-mentioned inoculation device and pushing device; the pushing device includes a receiving tray, a conveying tube and a second push rod; the conveying tube is extended along the first direction, the receiving tray is arranged on the side of the conveying tube, and a first feed port is opened at a position where the receiving tray and the conveying tube are in contact, and a second feed port is opened at a position of the conveying tube corresponding to the first feed port, and a side of the receiving tray away from the first feed port is higher than a side with the first feed port, so that the receiving tray is arranged with a slope, and the fungus carrier in the receiving tray can enter the conveying tube; the second push rod is arranged in the conveying tube, and can slide in the conveying tube along the first direction to push the fungus carrier entering the conveying tube into the plant stem.

[0018] Among them, the inoculation system provided by the present invention also includes a cutting knife, which is arranged in the receiving tray along the first direction and is located at the first feed port; one end of the cutting knife is rotatably connected to the receiving tray so that the cutting knife can cut the bacterial carrier in the receiving tray and seal the first feed port.

[0019] The inoculation system provided by the present application can make the fungus carrier enter the delivery tube through the first feed port and the second feed port by holding the fungus carrier in the holding tray, insert one end of the delivery tube into the inoculation wound, and then push the fungus carrier entering the delivery tube into the inoculation wound through the second push rod to complete the inoculation. Due to the use of the inoculation device provided by the present application, the size and depth of the inoculation wound formed on each plant stem are consistent, so that the amount of fungi entering the inoculation wound can be consistent, thereby improving the accuracy of the test.

[0020] The present application also provides an inoculation method, comprising the following steps: step S100, adjusting the position between the first limiting mechanism and the blade to determine the length of the blade protruding from the first limiting mechanism; step S200, inserting the blade into the plant stem until the end of the first limiting mechanism facing the blade abuts against the outer wall of the plant stem, so that an inoculation wound is formed on the plant stem; step S300, pushing the expansion mechanism into the accommodating cavity and moving in a direction close to the blade, and the second end of the first blade body and the second end of the second blade body expand in a direction away from each other to open the inoculation wound; step S400, pulling out the first blade body and the second blade body, keeping the expansion mechanism in the inoculation wound, inserting the delivery tube into the inoculation wound through the expansion mechanism, and pushing the fungus carrier in the receiving tray and entering the delivery tube into the inoculation wound through the second push rod; step S500, pulling out the delivery tube and the expansion mechanism in sequence, the inoculation wound retracts, wrapping the fungus carrier, and completing the inoculation.

[0021] The inoculation method of the present application uses the inoculation system provided by the present application. Through the inoculation device in the inoculation system, the size and depth of the inoculation wound caused to each plant stem can be guaranteed to be consistent, thereby ensuring the consistency of the inoculation cavity formed by the plant stem to a certain extent, and then ensuring the consistency of the inoculation amount. Through the push device in the inoculation system, the amount of the fungus carrier entering the inoculation cavity can be kept consistent, thereby further improving the accuracy of the amount of inoculated fungi. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 A schematic diagram of the overall structure of the vaccination device provided by an embodiment of the present invention from a first perspective;

[0024] Figure 2 A schematic diagram of a partial structure of the vaccination device provided by an embodiment of the present invention from a second viewing angle;

[0025] Figure 3 A schematic diagram of the structure of the first blade body and the second blade body in the inoculation device provided by an embodiment of the present invention in an expanded state;

[0026] Figure 4 A schematic diagram of the structure of an expansion component in an inoculation device provided in an embodiment of the present invention;

[0027] Figure 5 A schematic diagram of the structure of a handle in an inoculation device provided in an embodiment of the present invention;

[0028] Figure 6 A schematic diagram of the structure of a push device in an inoculation system provided in an embodiment of the present invention;

[0029] Figure 7 A schematic diagram of the process of the inoculation method provided in an embodiment of the present invention.

[0030] In the figure: 1-first blade body; 2-second blade body; 3-inlet end; 4-accommodating cavity; 401-slideway; 5-expansion member; 501-first expansion sheet; 502-second expansion sheet; 503-connecting rod; 5031-groove portion; 5032-connecting hole; 6-blade portion; 7-first positioning seat; 8-first limiting rod; 801-abutment portion; 9-first push rod; 10-connecting block; 11-second positioning seat; 12-second limiting rod; 13-positioning block; 14-handle; 1401-first gripping portion; 1402-second gripping portion; 1403-connecting piece; 15-receiving plate; 1501-first feed port; 16-delivery pipe; 17-second push rod; 18-cutting blade;

[0031] S1-first direction; S2-second direction. DETAILED DESCRIPTION

[0032] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the system or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0034] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0035] Figure 1 A schematic diagram of the overall structure of the vaccination device provided by an embodiment of the present invention from a first perspective; Figure 2 A schematic diagram of a partial structure of the vaccination device provided by an embodiment of the present invention from a second viewing angle; Figure 3 A schematic diagram of the structure of the first blade body and the second blade body in the inoculation device provided by an embodiment of the present invention in an expanded state; Figure 4 This is a schematic diagram of the structure of an expansion component in the vaccination device provided in an embodiment of the present invention.

[0036] like Figure 1-Figure 4As shown, the present invention provides an inoculation device, comprising a first blade body 1, a second blade body 2 and an expansion mechanism; the first blade body 1 and the second blade body 2 are arranged relative to each other along a first direction S1, a receiving cavity 4 is formed between the first blade body 1 and the second blade body 2, and the first end of the first blade body 1 and the first end of the second blade body 2 jointly form an inlet end 3 of the receiving cavity 4; the first end to the second end of the first blade body 1 and the first end to the second end of the second blade body 2 are both gradually contracted, and the second end of the first blade body 1 and the second end of the second blade body 2 are fitted together to form a blade portion 6; the expansion mechanism can enter the receiving cavity 4 through the inlet end 3 and move in a direction close to the blade portion 6, so that the second end of the first blade body 1 and the second end away from the second blade body 2 move relative to each other in directions away from each other, so as to achieve expansion of the blade portion 6.

[0037] The first limiting mechanism is arranged along the first direction S1 on the side of the first blade body 1 and / or the second blade body 2 away from the accommodating cavity 4, and one end of the first limiting mechanism facing the blade 6 is the limiting end, and there is a predetermined distance between the limiting end and the blade 6, so that when the blade 6 penetrates into the plant stem to a predetermined depth, one end of the first limiting mechanism can be against the outer wall surface of the plant stem.

[0038] Compared with the prior art, the inoculation device provided by the present invention has the following advantages:

[0039] The inoculation device provided by the present invention can penetrate the plant stem to form an inoculation wound by the blade 6 formed by the first blade body 1 and the second blade body 2. Since the first limiting mechanism is provided on the first blade body 1 or the second blade body 2, and when the blade 6 penetrates a certain depth into the plant stem, the first limiting mechanism can abut against the outer wall surface of the plant stem, so the penetration depth of the blade 6 can be limited.

[0040] The accommodating cavity 4 formed by the relatively arranged first blade body 1 and the second blade body 2 can enable the expansion mechanism to move from the inlet end 3 toward the direction close to the blade portion 6 formed by the first blade body 1 and the second blade body 2, so that the first blade body 1 and the second blade body 2 can expand in the direction away from each other, thereby expanding the inoculation wound.

[0041] Without changing the first limiting mechanism and the expansion mechanism, the depth of the blade 6 penetrating into each plant stem can be kept consistent to a certain extent, and the expansion size of the inoculation wound caused by the expansion mechanism can also be kept consistent to a certain extent, so that the size and depth of the inoculation cavity formed by each plant stem can be kept consistent to a certain extent, thereby achieving the unification of wound size and inoculation depth, and improving the accuracy of the test.

[0042] When in use, first adjust the end of the first limiting mechanism facing the blade 6 to an appropriate position. The distance between the end of the first limiting mechanism facing the blade 6 and the tip of the blade 6 is the depth of penetration into the plant stem.

[0043] Then, the blade 6 is inserted into the plant stem until the first limiting mechanism abuts against the outer wall of the plant stem. The expansion mechanism located at the inlet end 3 of the accommodating cavity 4 is gradually pushed toward the blade 6 at the front end. Since the first blade body 1 and the second blade body 2 are arranged to gradually converge from the inlet end 3 to the blade 6, the accommodating cavity 4 formed by the relative arrangement of the first blade body 1 and the second blade body 2 is also gradually converging, that is, the accommodating cavity 4 in the present application is V-shaped. The expansion mechanism entering from the inlet end 3 can expand the first end of the first blade body 1 and the first end of the second blade body 2 in a direction away from each other, thereby opening the inoculation wound on the plant stem.

[0044] Since the size of the expansion mechanism remains unchanged, the size of the inoculation wound opened by the expansion mechanism can be kept consistent to a certain extent. Since the distance between the first limiting mechanism and the blade 6 remains unchanged, the size of the inoculation cavity formed in each plant stem can be kept consistent to a certain extent, thereby improving the accuracy of the overall inoculation and the success rate of the test.

[0045] In the present application, the first end of the first blade body 1 and the first end of the second blade body 2 form a blade portion 6, that is, an end that can penetrate into the plant stem, and the second end of the first blade body 1 and the second end of the second blade body 2 are ends away from the blade portion 6, that is, an end where the expansion mechanism can extend into the accommodating cavity 4.

[0046] It should be noted that in the present application, scales may be provided on the side walls of the first blade body 1 and the second blade body 2 facing away from the accommodating cavity 4. The scales extend from the tip of the blade 6 to the inlet end 3 and are arranged at intervals of 1 mm.

[0047] In the present application, before the blade 6 penetrates into the plant stem, it may be disinfected to ensure that the penetration of the first blade body 1 and the second blade body 2 will not affect the fungi inoculated later on the plant stem.

[0048] Preferably, the first blade body 1 and the second blade body 2 in the present application are made of a metal material with certain elasticity and toughness, and the structure of the first blade body 1 and the second blade body 2 can achieve the purpose of expanding the first blade body 1 and the second blade body 2 in a direction away from each other.

[0049] It should be supplemented here that the cross-section of the first end of the first blade body 1 and the first end of the second blade body 2 in the second direction S2 in the present application is C-shaped, and the first blade body 1 and the second blade body 2 arranged opposite to each other along the first direction S1 have a certain interval, so as to form a slideway 401 above the accommodating groove along the first direction S1. The lower ends of the first blade body 1 and the second blade body 2 in the second direction S2 are connected, and the length of the connection is less than the length of the first blade body 1 and the second blade body 2, so as to meet the requirement that when the expansion mechanism moves to the front blade 6, the second end of the first blade body 1 and the second end of the second blade body 2 can expand in a direction away from each other.

[0050] Among them, Figure 1-Figure 4 As shown, the length of the first blade body 1 in the first direction S1 is greater than the length of the second blade body 2, and the first end of the first blade body 1 and the first end of the second blade body 2 are arranged flush, and the second end of the first blade body 1 protrudes from the second end of the second blade body 2; the first limiting mechanism includes a first positioning seat 7 and a first limiting rod 8; the first positioning seat 7 is arranged on the first blade body 1, and the first limiting rod 8 is arranged along the first direction S1 and is movably connected to the first positioning seat 7 so that one end of the first limiting rod 8 can move in the direction close to or away from the plant stem; the end of the first limiting rod 8 facing the blade 6 is formed as the limiting end, and the end face of the limiting end is an arc surface.

[0051] In the present application, the arc-shaped limiting end forms an abutment portion 801 that can fit against the outer wall of the plant stem.

[0052] Preferably, in the present application, the length of the first blade body 1 is greater than the length of the second blade body 2. Since the first end of the first blade body 1 and the first end of the second blade body 2 are arranged flush with each other, the second end of the first blade body 1 can protrude from the second end of the first blade body 1, thereby enabling the formed blade 6 to better penetrate into the plant stem.

[0053] In the present application, the first limiting mechanism includes a first positioning seat 7 and a first limiting rod 8. Since the first blade body 1 protrudes from the second blade body 2, when piercing the plant stem, the first blade body 1 first enters the stem body. Therefore, the first positioning seat 7 is set on the first blade body 1 to limit the position of the second end of the first blade body 1.

[0054] In the present application, a screw hole penetrating the first positioning seat 7 is opened on the first positioning seat 7 along the first direction S1, and the first limiting rod 8 is a screw rod. By rotating the first limiting rod 8, the abutment portion 801 can be moved toward or away from the plant stem, thereby limiting the penetration depth of the blade 6.

[0055] Preferably, the abutment portion 801 formed at one end of the first limiting rod 8 facing the plant stem in the present application can better fit with the plant stem, thereby further improving the accuracy of the penetration depth.

[0056] Specifically, Figure 1-Figure 4 As shown, the expansion mechanism includes an expansion component 5 and a first push rod 9; the size of the expansion component 5 matches the size of the inlet end 3 of the accommodating cavity 4, and the expansion component includes two expansion surfaces that are arranged back to back and have a certain distance between them, and the two expansion surfaces are respectively abutted against the inner wall surfaces of the first blade body 1 and the second blade body 2; the first push rod 9 is used to push the expansion mechanism toward the direction close to the blade portion 6.

[0057] Preferably, the expansion member 5 in the present application is an H-shaped structure, and the H-shaped expansion mechanism can stably move in the accommodating cavity 4, and make the first blade body 1 and the second blade body 2 stably move away from each other. In addition, since the fungus carrier needs to enter the inoculation wound of the plant stem through the delivery tube 16, the H-shaped expansion mechanism can also facilitate the insertion of the delivery tube 16.

[0058] It should be additionally explained here that, since the second limiting mechanism is arranged above the connecting rod 503, when it is necessary to deliver the bacterial carrier into the inoculation wound through the delivery tube 16, one end of the delivery tube 16 enters the inoculation wound through the square channel formed below the H-shaped expansion mechanism.

[0059] Furthermore, if Figure 1-Figure 4 As shown, the expansion member 5 includes a first expansion piece 501, a second expansion piece 502 and a connecting rod 503; the first expansion piece 501 is correspondingly fitted with the first blade body 1, the second expansion piece 502 is correspondingly fitted with the second blade body 2, a groove portion 5031 is formed on the connecting rod 503, and the first push rod 9 can be inserted into the groove portion 5031 to push the expansion member 5; a connecting hole 5032 is opened in the groove portion 5031 along the first direction S1, and a connecting portion is formed at one end of the first push rod 9 facing the groove portion 5031, and the connecting portion can be inserted into the connecting hole 5032 to connect the first push rod 9 to the expansion member 5.

[0060] In the present application, the first expansion piece 501 and the second expansion piece 502 are located at both ends of the connecting rod 503 to form an H-shaped structure. The connecting rod 503 makes the first expansion piece 501 correspond to and fit with the inner wall surface of the first blade body 1, and the second expansion piece 502 corresponds to and fits with the inner wall surface of the second blade body 2, so that the process of the first blade body 1 and the second blade body 2 moving away from each other can be made more stable.

[0061] Since a groove portion 5031 is formed on the connecting rod 503 in the present application, when the expansion member 5 is pushed toward the blade portion 6 by the first push rod 9, the first push rod 9 can be inserted into the groove portion 5031. On the one hand, the first push rod 9 and the expansion member 5 can be quickly and accurately aligned. On the other hand, the process of the first push rod 9 pushing the expansion member 5 can be made more stable.

[0062] The length of the first push rod 9 in the present application is greater than the length of the accommodating cavity 4. When the blade 6 penetrates the plant stem to cause an inoculation wound, the first push rod 9 pushes the expansion member 5 toward the blade 6, so that the first blade body 1 and the second blade body 2 move away from each other to open the inoculation wound. Afterwards, the expansion member 5 is pressed against by the first push rod 9, and the first blade body 1 and the second blade body 2 are pulled to separate the blade 6 from the first expansion piece 501 and the second expansion piece 502, and the first push rod 9, the first blade body 1 and the second blade body 2 are removed.

[0063] The square channel formed by the expansion component 5 of the conveying tube 16 is inserted into the inoculation wound. After the fungus carrier is pushed in, the conveying tube 16 is first pulled out for a distance to separate the conveying tube 16 from the plant stem, and then the second push rod 17 is pulled out, thereby completing the conveying of the fungus carrier and avoiding, to a certain extent, the problem of the fungus carrier being taken out when the conveying tube 16 is pulled out.

[0064] It should be noted here that the connection hole 5032 in the present application is a screw hole, and a screw that can be screwed into the screw hole is formed at the front end of the first push rod 9. When the bacterial carrier is transported into the inoculation wound and the delivery tube 16 is pulled out, the screw formed at the front end of the first push rod 9 and the screw hole are threadedly connected, so that the first push rod 9 can be connected to the expansion member 5, so that the expansion member 5 can be pulled out of the inoculation wound.

[0065] Furthermore, if Figure 1-Figure 4 As shown, the inoculation device provided by the present invention also includes a second limiting mechanism, which includes a connecting block 10, a second positioning seat 11 and a second limiting rod 12; one end of the connecting block 10 is connected to the connecting rod 503, and the other end extends out of the accommodating cavity 4; the second positioning seat 11 is arranged at one end of the connecting block 10 extending out of the accommodating cavity 4, and the second limiting rod 12 is movably connected to the second positioning seat 11 along the first direction S1; the second limiting rod 12 can follow the expansion member 5 to move in the direction close to the blade 6, and one end of the second limiting rod 12 can be against the outer wall of the plant stem to limit the depth of the expansion member 5 entering the plant stem.

[0066] One end of the connecting block 10 is connected to the connecting rod 503, and the other end is extended out of the accommodating cavity 4 by the slide 401. The second positioning seat 11 is arranged on the end of the connecting block 10 extending out of the accommodating cavity 4, and the second limiting rod 12 is arranged along the first direction S1 through the second positioning seat 11.

[0067] Since the expansion mechanism moves in the accommodating cavity 4, the length of the expansion member 5 protruding from the blade 6 can be limited by providing the second limiting rod 12, thereby avoiding the problem of the expansion mechanism being completely pushed into the inoculation wound causing the inoculation wound to be too large.

[0068] When in use, the second limiting rod 12 moves toward the plant stem as the expansion member 5 moves. When one end of the second limiting rod 12 contacts the plant stem, the pushing can be stopped, thereby limiting the pushing depth of the expansion member 5.

[0069] Figure 5 This is a schematic diagram of the structure of a handle in an inoculation device provided in an embodiment of the present invention.

[0070] Among them, Figure 1-Figure 5 As shown, the vaccination device provided by the present invention also includes a positioning block 13 and a handle 14; the positioning block 13 is arranged along the second direction S2, and the first end of the first blade body 1 and the first end of the second blade body 2 are both connected to one end of the positioning block 13; the other end of the positioning block 13 is connected to the handle 14.

[0071] In the present application, the first end of the first blade body 1 and the first end of the second blade body 2 are integrally formed with the positioning block 13, and the end of the positioning block 13 away from the first blade body 1 is connected to the handle 14. By setting the handle 14, it is convenient for the operator to hold and operate the vaccination device.

[0072] In the above embodiment, the first blade body 1, the second blade body 2 and the positioning block 13 are arranged in an L shape, that is, the first direction S1 and the second direction S2 in the present application are perpendicular to each other. The first blade body 1, the second blade body 2 and the positioning block 13 are arranged in an L shape, which can better control the wound state caused by piercing the plant stem and improve the operation accuracy.

[0073] Preferably, in the present application, a receiving groove is formed at one end of the positioning block 13 away from the handle, and the first end of the first blade body 1 and the first end of the second blade body 2 are disposed in the receiving groove and are rotatably connected to the positioning block 13 via a rotating shaft.

[0074] During operation, the first end of the first blade body 1 and the first end of the second blade body 2 are rotated relative to the positioning block 13, and the angle formed by the first blade body 1 and the second blade body 2 and the positioning block 13 is adjusted according to the operation requirements. Preferably, the first blade body 1 and the second blade body 2 are in a horizontal state with the positioning block 13. Then, the handle 14 is held, and the first blade body 1 and the second blade body 2 are inserted into the plant stem through the blade 6, and the first blade body 1 and the second blade body 2 are rotated again to expose the inlet end of the accommodating cavity 4, and the expansion mechanism is pushed into the accommodating cavity 4 to make the second end of the first blade body 1 and the second end of the second blade body 2 move away from each other.

[0075] Further preferably, the handle 14 in the present application is hollow and consists of a first gripping portion 1401 and a second gripping portion 1402. The ends of the first gripping portion 1401 and the second gripping portion 1402 away from the positioning block 13 are connected by a connecting member 1403 and can rotate relative to the connecting member 1403 so that the first gripping portion 1401 and the second gripping portion 1402 can rotate in a direction away from each other. The end of the first gripping portion 1401 away from the connecting member 1403 is rotatably connected to the positioning block 13, and the end of the second gripping portion 1402 away from the connecting member 1403 is in contact with the positioning block 13.

[0076] When the operation is completed, the first blade body 1 and the second blade body 2 are first rotated to be horizontal with the positioning block 13, and then the first gripping portion 1401 and the second gripping portion 1402 are pulled away from each other. Since the handle 14 is hollow and the positioning block 13 is rotatably connected to the first gripping portion 1401, the positioning block 13, the first blade body 1 and the second blade body 2 can be turned into the handle 14 together, and the second gripping portion 1402 can be moved in a direction close to the first gripping portion 1401, thereby realizing the recovery and protection of the first blade body 1 and the second blade body 2.

[0077] Since the first blade body 1 and the second blade body 2 can be rotated and received in the handle 14 which is arranged in a hollow shape, the first blade body 1 and the second blade body 2 can be protected to a certain extent, thereby improving the service life of the whole device.

[0078] Figure 6 A schematic diagram of the structure of a pushing device in an inoculation system provided in an embodiment of the present invention.

[0079] In addition, if Figure 1-Figure 6 As shown, the present invention also provides an inoculation system, including the above-mentioned inoculation device and the pushing device; the pushing device includes a receiving tray 15, a conveying tube 16 and a second push rod 17; the conveying tube 16 is extended along the first direction S1, the receiving tray 15 is arranged on the side of the conveying tube 16, and a first feed port 1501 is opened at a position where the receiving tray 15 and the conveying tube 16 are in contact, and a second feed port is opened at a position of the conveying tube 16 corresponding to the first feed port 1501, so that the fungus carrier in the receiving tray 15 can enter the conveying tube 16; the second push rod 17 is arranged in the conveying tube 16, and can slide in the conveying tube 16 along the first direction S1 to push the fungus carrier entering the conveying tube 16 into the plant stem.

[0080] The inoculation system provided by the present application can make the fungus carrier enter the conveying tube 16 through the first feed port 1501 and the second feed port by holding the fungus carrier in the holding tray 15, insert one end of the conveying tube 16 into the inoculation wound, and then push the fungus carrier entering the conveying tube 16 into the inoculation wound through the second push rod 17 to complete the inoculation. Due to the use of the inoculation device provided by the present application, the size and depth of the inoculation wound formed on each plant stem are consistent, so that the amount of fungi entering the inoculation wound can be consistent, thereby improving the accuracy of the test.

[0081] Among them, Figure 1-Figure 6 As shown, the height of the side of the receiving tray 15 away from the first feed opening 1501 is higher than the side with the first feed opening 1501, so that the receiving tray 15 is arranged in a slope.

[0082] Preferably, the height of the side of the holding tray 15 away from the first feed port 1501 in the present application is higher than the side with the first feed port 1501 so that the fungus carrier can move toward the first feed port 1501, thereby allowing the fungus carrier to automatically enter the conveying pipe 16.

[0083] After the bacterial carrier enters the delivery tube 16, the bacterial carrier is pushed into the inoculation wound by the second push rod 17. Since the second push rod 17 can block the second feed port during the pushing process, the bacterial carrier will not enter the delivery tube 16 again, thereby ensuring the consistency of the delivery amount of the bacterial carrier to a certain extent, thereby improving the precision and accuracy of the test.

[0084] Specifically, Figure 1-Figure 6 As shown, the inoculation system provided by the present invention also includes a cutting knife 18, which is arranged in the receiving tray 15 along the first direction S1 and is located at the first feed port 1501; one end of the cutting knife 18 is rotatably connected to the receiving tray 15 so that the cutting knife 18 can cut the bacterial carrier in the receiving tray 15 and block the first feed port 1501.

[0085] Since some fungus carriers need to be cut, the present application preferably provides a cutting knife 18 at the position of the first feed port 1501, and since one end of the cutting knife 18 is rotatably connected to the carrying plate, after the cutting knife 18 cuts the fungus carrier, the remaining fungus carriers in the carrying plate can be sealed outside the first feed port 1501, thereby further ensuring the delivery rate of the fungus carriers.

[0086] Figure 7 A schematic diagram of the process of the inoculation method provided in an embodiment of the present invention.

[0087] like Figure 7As shown, the present invention also provides an inoculation method, comprising the following steps: step S100, adjusting the position between the first limiting mechanism and the blade 6, and determining the length of the blade 6 protruding from the first limiting mechanism; step S200, inserting the blade 6 into the plant stem until one end of the first limiting mechanism facing the blade 6 abuts against the outer wall surface of the plant stem, so that an inoculation wound is formed on the plant stem; step S300, pushing the expansion mechanism into the accommodating cavity 4, and moving in a direction close to the blade 6, the second end of the first blade body 1 and the second end of the second blade body 2 expand in a direction away from each other, so as to open the inoculation wound; step S400, pulling out the first blade body 1 and the second blade body 2, inserting the delivery tube 16 into the inoculation wound, and pushing the fungus carrier entering the delivery tube 16 in the receiving tray into the inoculation wound through the second push rod 17; step S500, pulling out the delivery tube 16 and the expansion mechanism in sequence, the inoculation wound retracts, wrapping the fungus carrier, and completing the inoculation.

[0088] The inoculation method of the present application uses the inoculation system provided by the present application. Through the inoculation device in the inoculation system, the size and depth of the inoculation wound caused to each plant stem can be guaranteed to be consistent, thereby ensuring the consistency of the inoculation cavity formed by the plant stem to a certain extent, and then ensuring the consistency of the inoculation amount. Through the push device in the inoculation system, the amount of the fungus carrier entering the inoculation cavity can be kept consistent, thereby further improving the accuracy of the amount of inoculated fungi.

[0089] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A vaccination device, characterized in that: It includes a first blade body, a second blade body and an expansion mechanism; The first blade body and the second blade body are arranged opposite to each other along a first direction, a receiving cavity is formed between the first blade body and the second blade body, and a first end of the first blade body and a first end of the second blade body jointly form an inlet end of the receiving cavity; The first end to the second end of the first blade body and the first end to the second end of the second blade body are both tapered, and the second end of the first blade body and the second end of the second blade body are fitted together to form a blade portion; The expansion mechanism can enter the accommodating cavity through the inlet end and move in a direction close to the blade portion, so that the second end of the first blade body and the second end of the second blade body move relative to each other in directions away from each other, so as to achieve expansion of the blade portion; The side walls of the first blade body and the second blade body on the side away from the accommodating cavity are both provided with scales, and the scales extend from the tip of the blade portion to the inlet end.

2. The inoculation device according to claim 1, characterized in that: It also includes a first limiting mechanism, which is arranged along a first direction on a side of the first blade body and / or the second blade body away from the accommodating cavity, and one end of the first limiting mechanism facing the blade is a limiting end, and there is a predetermined distance between the limiting end and the blade, so that when the blade penetrates into a predetermined depth in the plant stem, one end of the first limiting mechanism can abut against the outer wall surface of the plant stem.

3. The inoculation device according to claim 2, characterized in that: The length of the first blade body in the first direction is greater than that of the second blade body, and along the first direction, the first end of the first blade body is flush with the first end of the second blade body, and the second end of the first blade body protrudes beyond the second end of the second blade body; The first limiting mechanism includes a first positioning seat and a first limiting rod; The first positioning seat is arranged on the first blade body, and the first limiting rod is arranged along the first direction and is movably connected to the first positioning seat so that one end of the first limiting rod can move toward or away from the blade; One end of the first limiting rod facing the blade portion forms a limiting end, and an end surface of the limiting end is an arc surface.

4. The inoculation device according to claim 1, characterized in that: The expansion mechanism includes an expansion member and a first push rod; The size of the expansion member matches the size of the inlet end of the accommodating cavity, and the expansion mechanism includes two expansion surfaces that are arranged opposite to each other and have a certain distance, and the two expansion surfaces are respectively in contact with the inner wall surfaces of the first blade body and the second blade body; The first push rod is used to push the expansion mechanism toward the direction close to the blade portion.

5. The inoculation device according to claim 4, characterized in that: The expansion member includes a first expansion piece, a second expansion piece and a connecting rod; The first expansion piece is correspondingly fitted with the first blade body, the second expansion piece is correspondingly fitted with the second blade body, a groove portion is formed on the connecting rod, and the first push rod can be inserted into the groove portion to push the expansion member; A connecting hole is formed in the groove portion along the first direction, and a connecting portion is formed at one end of the first push rod facing the groove portion. The connecting portion can be inserted into the connecting hole to connect the first push rod to the expansion member.

6. The inoculation device according to claim 5, characterized in that: It also includes a second limiting mechanism, which includes a connecting block, a second positioning seat and a second limiting rod; One end of the connecting block is connected to the connecting rod, and the other end extends out of the accommodating cavity; The second positioning seat is arranged at one end of the connecting block extending out of the accommodating cavity, and the second limiting rod is movably connected to the second positioning seat along the first direction; The second limiting rod can follow the expansion member to move toward the cutting edge, and one end of the second limiting rod can abut against the outer wall of the plant stem to limit the depth of the expansion member entering the plant stem.

7. The inoculation device according to claim 1, characterized in that: Also includes a positioning block and a handle; The positioning block is arranged along the second direction, and the first end of the first blade body and the first end of the second blade body are both connected to the positioning block; One end of the positioning block away from the first blade body and the second blade body is connected to the handle.

8. An inoculation system, characterized in that: The inoculation device and the pushing device comprising any one of claims 1 to 7; The pushing device comprises a receiving plate, a conveying pipe and a second push rod; The conveying pipe is extended along the first direction, the receiving tray is arranged on the side of the conveying pipe, and a first feed inlet is opened at a position where the receiving tray and the conveying pipe are in contact, and a second feed inlet is opened at a position of the conveying pipe corresponding to the first feed inlet, and a side of the receiving tray away from the first feed inlet is higher than a side where the first feed inlet is opened, so that the receiving tray is arranged with a slope, and the fungus carrier in the receiving tray can enter the conveying pipe; The second push rod is disposed in the conveying tube and can slide in the conveying tube along the first direction to push the fungus carrier entering the conveying tube into the plant stem.

9. The inoculation system according to claim 8, characterized in that: It also includes a cutting knife, which is arranged in the receiving tray along the first direction and located at the first feed inlet; One end of the cutting knife is rotatably connected to the holding tray, so that the cutting knife can cut the fungus carrier in the holding tray and block the first feed port.

10. A method for inoculation using the inoculation system provided in claim 8, characterized in that: The steps include: Step S100, adjusting the position between the first limiting mechanism and the blade to determine the length of the blade protruding from the first limiting mechanism; Step S200, inserting the blade into the plant stem until one end of the first limiting mechanism facing the blade abuts against the outer wall of the plant stem, so that an inoculation wound is formed on the plant stem; Step S300, pushing the expansion mechanism into the accommodating cavity and moving it in a direction close to the blade portion, so that the second end of the first blade body and the second end of the second blade body expand in a direction away from each other to open the inoculation wound; Step S400, extracting the first blade body and the second blade body, keeping the expansion mechanism in the inoculation wound, inserting the delivery tube into the inoculation wound through the expansion mechanism, and pushing the bacterial carrier in the receiving tray and into the delivery tube into the inoculation wound through the second push rod; Step S500, pull out the delivery tube and the expansion mechanism in sequence, the inoculation wound shrinks, wraps the bacterial carrier, and the inoculation is completed.

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