A device for quantitatively measuring the light preference of aquatic organisms

Abstract

The present invention relates to a device for quantitatively measuring the light preference of aquatic organisms, comprising a plurality of variable spectrum lamps and a plurality of boxes arranged in a ring; each box comprises a first containing slot and a second containing slot arranged on the first containing slot; each variable spectrum lamp is arranged in each first containing slot; one end of each second containing slot is provided with a switchable partition, a plurality of partitions enclose an aquatic organism adaptation area, and a plurality of second containing slots constitute an aquatic organism selection area; during measurement, each variable spectrum lamp emits light of a different spectrum, the partition is in an open state, and the aquatic organism to be measured moves from the aquatic organism adaptation area to the aquatic organism selection area, completing the quantitative measurement of the light preference of the aquatic organism. The present invention arranges the variable spectrum lamp in the first containing slot, and while obtaining a uniform light field underwater, the spectrum and light intensity can also be accurately adjusted to improve the measurement effect of the light preference of aquatic organisms.

Description

Technical Field

[0001] The invention relates to the technical field of aquatic organism light preference determination, and in particular to a device for quantitatively determining the light preference of aquatic organisms. Background Art

[0002] Fish behavior is a discipline that studies the interaction between fish and the environment and other organisms. It has important guiding significance for fish resource protection, fishery aquaculture production, etc. Fish preference behavior is an effective indicator to measure the selection and preference of fish for different thresholds of environmental factors. It is an important content in the research of fish natural habitat protection, fish passage facility design, welfare aquaculture, etc. The study of fish preference behavior is based on multiple factors such as the distribution frequency, movement distance and speed of fish in various environmental factors. Therefore, the ability to accurately and precisely track fish in the experiment is of great significance to the effectiveness of subsequent analysis and results.

[0003] Lighting is a common problem in the study of fish behavior preference. However, in the process of video acquisition of fish behavior, the baffle in the test area often blocks the fish's movement trajectory, resulting in experimental errors when the software is tracking. Manual correction is required for each test, which consumes a lot of manpower and time, affecting the progress of the test. Moreover, the light used in light preference tests is often a fixed wavelength light source, and the degree of attenuation in water is often not considered.

[0004] Therefore, how to greatly reduce the impact of human factors and accurately adjust the spectrum and light intensity while obtaining a uniform light field underwater, thereby promoting the study of light preference behavior of aquatic organisms is a technical problem that urgently needs to be solved. Summary of the invention

[0005] The purpose of the present invention is to provide a device for quantitatively measuring the light preference of aquatic organisms, which can accurately adjust the spectrum and light intensity while obtaining a uniform light field underwater, thereby improving the accuracy of measuring the light preference of aquatic organisms.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] A device for quantitatively determining the light preference of aquatic organisms, comprising a plurality of variable spectrum lamps and a plurality of boxes arranged in a ring;

[0008] Each of the boxes comprises a first containing slot and a second containing slot arranged on the first containing slot; each of the variable spectrum lamps is arranged in each of the first containing slots;

[0009] One end of each of the second containing tanks is provided with a switchable partition, and when the partition is in a closed state, the plurality of partitions enclose an aquatic organism adaptation area; the plurality of the second containing tanks constitute an aquatic organism selection area;

[0010] During measurement, each of the variable spectrum lamps emits light of a different spectrum, the partition is in an open state, and the aquatic organisms to be measured move from the aquatic organism adaptation area to the aquatic organism selection area, thereby completing the quantitative measurement of the aquatic organism light preference.

[0011] Optionally, a lens and a camera are also included;

[0012] The lens is arranged above the plurality of boxes; and the camera is arranged above the lens.

[0013] Optionally, a detachable bracket is further included, and the detachable bracket is arranged below the plurality of boxes.

[0014] Optionally, the number of the boxes is six, the boxes are rectangular boxes, and the angle between two adjacent boxes is 60 degrees.

[0015] Optionally, the box body is made of white PVC board.

[0016] Optionally, the variable spectrum lamp includes a lampshade and lamp beads arranged in the lampshade, the lampshade is a translucent acrylic cover, and the lamp beads are RGB lamp beads, LED lamp beads, IR LED lamp beads or 3030 packaged lamp beads with different wavelengths.

[0017] Optionally, a dimming knob is arranged outside the lampshade.

[0018] Optionally, a plurality of box slots are provided above the detachable bracket, and a plurality of boxes are respectively provided on the plurality of box slots; and a roller is provided below the detachable bracket.

[0019] Optionally, the detachable bracket is made of an aluminum alloy metal sheet, and the roller is made of a plastic material.

[0020] According to the specific embodiments provided by the present invention, the present invention discloses the following technical effects:

[0021] The present invention discloses a device for quantitatively measuring the light preference of aquatic organisms, comprising a plurality of variable spectrum lamps and a plurality of boxes arranged in a ring; each of the boxes comprises a first containing slot and a second containing slot arranged on the first containing slot; each of the variable spectrum lamps is arranged in each of the first containing slots; one end of each of the second containing slots is provided with a switchable partition, a plurality of the partitions enclose an aquatic organism adaptation area, and a plurality of the second containing slots constitute an aquatic organism selection area; during measurement, each of the variable spectrum lamps emits light of a different spectrum, the partitions are in an open state, and the aquatic organisms to be measured move from the aquatic organism adaptation area to the aquatic organism selection area, thereby completing the quantitative measurement of the light preference of the aquatic organisms. The present invention arranges the variable spectrum lamp in the first containing slot, and while obtaining a uniform light field underwater, it can also accurately adjust the spectrum and light intensity, thereby improving the measurement effect of the light preference of aquatic organisms. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0023] Figure 1 A structural diagram of a device for quantitatively measuring the light preference of aquatic organisms provided by the present invention;

[0024] Figure 2 This is a schematic diagram of the detachable bracket provided by the present invention.

[0025] Explanation of symbols:

[0026] 1-box, 11-second containing tank, 12-first containing tank, 2-partition, 3-aquatic life adaptation area, 4-aquatic life selection area, 5-lens, 6-camera, 7-detachable bracket. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] The purpose of the present invention is to provide a device for quantitatively measuring the light preference of aquatic organisms, which can accurately adjust the spectrum and light intensity while obtaining a uniform light field underwater, thereby improving the accuracy of measuring the light preference of aquatic organisms.

[0029] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0030] like Figure 1 As shown, the device for quantitatively measuring the light preference of aquatic organisms includes a plurality of variable spectrum lamps (not shown in the figure) and a plurality of boxes 1 arranged in a ring. Each box 1 includes a first holding slot 12 and a second holding slot 11 arranged on the first holding slot 12. Each variable spectrum lamp is arranged in each first holding slot 12. A switchable partition 2 is arranged at one end of each second holding slot 11. When the partition 2 is in the off state, the plurality of partitions 2 enclose an aquatic organism adaptation area 3. The plurality of second holding slots 11 constitute an aquatic organism selection area 4. During measurement, each variable spectrum lamp emits light of a different spectrum, the partition 2 is in the open state, and the aquatic organisms to be measured move from the aquatic organism adaptation area 3 to the aquatic organism selection area 4, thereby completing the quantitative measurement of the light preference of the aquatic organisms.

[0031] In this embodiment, the device for quantitatively measuring the light preference of aquatic organisms further includes a lens 5 and a camera 6. The lens 5 is arranged above the plurality of boxes 1. The camera 6 is arranged above the lens 5. The camera 6 is used to photograph the movement of the aquatic organisms to be measured, and the lens 5 is used to adjust the imaging effect of the camera 6.

[0032] In this embodiment, there are six boxes 1, each of which is a rectangular box, and the angle between two adjacent boxes 1 is 60 degrees, so that the six boxes 1 form a "hexagonal maze". The boxes 1 are made of white PVC boards.

[0033] In this embodiment, the variable spectrum lamp includes a lampshade and lamp beads arranged in the lampshade, the lampshade is a translucent acrylic cover, and the lamp beads are RGB lamp beads, LED lamp beads, IR LED lamp beads or 3030 package lamp beads with different wavelengths. The variable spectrum lamp can produce 12 different spectra. A dimming knob is arranged outside the lampshade.

[0034] Figure 2 Schematic diagram of the detachable bracket provided by the present invention, the device for quantitatively determining the light preference of aquatic organisms also includes a detachable bracket 7, and the detachable bracket 7 is arranged below the multiple boxes 1. Among them, multiple box card slots are arranged above the detachable bracket 7, and the multiple boxes 1 are respectively arranged on the multiple box card slots. A roller is arranged below the detachable bracket 7. The detachable bracket 7 is made of aluminum alloy metal sheet, and the roller is made of plastic material. This device is a detachable device that can be stored in a square box, which is convenient for indoor and outdoor sampling.

[0035] This example also provides the following light preference behavior test:

[0036] Light preference behavior test using yellow catfish as test fish:

[0037] Step 1: Preparation before the experiment. Build the device, fill the aquatic organism adaptation area 3 and the aquatic organism selection area 4 with water, adjust the dimming knob of the variable spectrum lamp, set white light, green light (wavelength 520nm), red light (wavelength 660nm), blue light (wavelength 430nm), orange light (wavelength 590nm) and purple light (wavelength 365nm), and adjust the light intensity to create the required environment.

[0038] Step 2: Fish preference test. Place a yellow catfish test fish in the aquatic organism adaptation area 3 for 30 minutes, open the six partitions 2, and use the camera 6 to observe and record the distribution of the fish in the second holding tank 11. Record continuously for 24 hours, and record the number of fish in each second holding tank 11 every 5 minutes.

[0039] Step 3: Experimental data collection and light preference calculation. Calculate the percentage of time that the yellow catfish test fish appears in each second holding tank 11 under white light, green light, red light, blue light, orange light and purple light during the recording time. The calculation formula is: P = (n / N) * 100%, where P is the time percentage, n is the time of appearance under a certain light, and N is the recording time. Each test fish is recorded for 24 hours, repeated for 6 groups, and the frequency percentage of the yellow catfish test fish appearing under white light, green light, red light, blue light, orange light and purple light is shown in Table 1.

[0040] Table 1

[0041] Group No. White light (%) Green light (%) Red light (%) Blue light (%) Orange light (%) Purple light (%) 1 22.5 14.4 16.7 15.1 14.8 16.5 2 26.6 15.0 14.6 15.7 13.7 14.4 3 22.8 15.9 15.7 15.2 15.5 14.9 4 21.9 16.8 14.4 15.2 16.6 15.1 5 23.3 15.0 16.1 15.2 13.7 16.7 6 26.2 14.6 15.8 16.3 13.5 13.6

[0042] Step 4: Maintenance and cleaning of the device. After the test, disassemble the device, clean and maintain it, and then store it in different categories.

[0043] Light preference behavior test using zebrafish as test fish:

[0044] Step 1: Preparation before the experiment. Build the device, fill the aquatic organism adaptation area 3 and the aquatic organism selection area 4 with water, adjust the dimming knob of the variable spectrum lamp, and arrange six light gradients: 0Lx, 25Lx, 50Lx, 75Lx, 100Lx and 125Lx (the light intensity is the result of the illuminance meter tested underwater) to create the required environment.

[0045] Step 2: Fish preference test. Place a zebrafish test fish in the aquatic organism adaptation area 3 for 30 minutes, open the six partitions 2, and use the camera 6 to observe and record the distribution of the fish in the second holding tank 11. Record continuously for 24 hours, and record the number of fish in each second holding tank 11 every 5 minutes.

[0046] Step 3: Calculate the active time, speed, and distance of the zebrafish test fish.

[0047] The moving distance of zebrafish = pixels in the previous frame - pixels in the next frame. When the moving distance of zebrafish is less than 20% of its body length, the movement can be defined as low activity. When the moving distance is greater than or equal to 20% of its body length and less than or equal to 80% of its body length, the movement can be defined as active. When the moving distance is greater than 80% of its body length, the movement is defined as high activity. The test results are shown in Table 2. The speed in Table 2 is the moving distance of zebrafish per second, and the total distance is the total distance moved during the recording time.

[0048] Table 2

[0049] parameter 0L 25Lx 50Lx 75Lx 100Lx 125Lx Low activity time (%) 40.3 29.2 16.3 8.7 3.4 2.1 Active time (%) 50.3 19.2 18.2 5.9 3.3 3.1 High activity time (%) 49.2 18.4 19.7 8.3 2.9 1.5 Speed ​​(cm / s) 4.9 5.6 8.0 7.9 11.8 3.3 Total distance (%) 30.1 16.6 13.8 9.2 10.3 20.0

[0050] Step 4: Maintenance and cleaning of the device. After the test, disassemble the device, clean and maintain it, and then store it in different categories.

[0051] Experiment on the movement of fish under different colors of light:

[0052] Step 1: Preparation before the experiment. Build the device, fill the aquatic organism adaptation area 3 and the aquatic organism selection area 4 with water, adjust the dimming knob of the variable spectrum lamp, set white light, green light (wavelength 520nm), red light (wavelength 660nm), blue light (wavelength 430nm), orange light (wavelength 590nm) and purple light (wavelength 365nm), and adjust the light intensity to create the required environment.

[0053] Step 2: Fish preference test. Place one test fish in the aquatic organism adaptation area 3 for 30 minutes, then open the six partitions 2 and use the camera 6 to observe and record the distribution of the fish in the second holding tanks 11. Record continuously for 24 hours, and record the number of fish in each second holding tank 11 every 5 minutes.

[0054] Step 3: Calculate the active time, speed, distance, etc. of the test fish.

[0055] The moving distance of the test fish = the pixel of the previous frame - the pixel of the next frame. When the moving distance of the test fish is less than 20% of its body length, the movement can be defined as low activity. When the moving distance is greater than or equal to 20% of its body length and less than or equal to 80% of its body length, the movement can be defined as active. When the moving distance is greater than 80% of its body length, the movement is defined as high activity. The test results are shown in Table 3. The speed in Table 3 is the moving distance of the test fish per second, and the total distance is the total distance moved during the recording time.

[0056] Table 3

[0057] parameter White light Red light Green Light Blu-ray Orange Light Purple light Low activity time (%) 24.8 16.7 15.3 14.9 14.2 14.0 Active time (%) 26.6 15.2 14.0 15.5 13.5 15.2 High activity time (%) 22.9 15.3 15.9 14.6 15.0 16.3 Speed ​​(cm / s) 1.9 15.6 18.0 8.9 3.8 13.3 Total distance (%) 30.3 17.3 10.9 3.7 6.8 9.6

[0058] Step 4: Maintenance and cleaning of the device. After the test, disassemble the device, clean and maintain it, and then store it in different categories.

[0059] The present invention arranges a lens above the box body, and arranges a camera above the lens. The imaging effect of the camera is adjusted by the lens, so as to realize panoramic recording of the light preference of aquatic organisms, and effectively improves the phenomenon in the prior art that the baffle covers the movement trajectory of aquatic organisms during shooting and recording.

[0060] In the prior art, light strips or waterproof point light sources are often used, which often result in interference from the light source environment (such as slight flickering of the light), and the test light intensity cannot be controlled, and a uniform light field cannot be formed, which easily leads to test errors, and the test cost is also very high. The present invention sets a variable spectrum lamp in the first holding tank to achieve water-light isolation, and while obtaining a uniform light field underwater, the spectrum and light intensity can be accurately adjusted, thereby improving the effect of determining the light preference of aquatic organisms and reducing the test cost.

[0061] The variable spectrum lamp of the present invention can generate 12 different adjustable spectra, which can meet the test needs and improve the light preference measurement effect compared with the four spectra of white light, green light, red light and blue light in the prior art with fixed light colors.

[0062] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0063] The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A device for quantitatively determining the light preference of aquatic organisms, characterized in that: It includes a plurality of variable spectrum lamps, a plurality of boxes arranged in a ring, a detachable bracket, a lens and a camera; Each of the boxes comprises a first containing slot and a second containing slot arranged on the first containing slot; each of the variable spectrum lamps is arranged in each of the first containing slots; One end of each of the second containing tanks is provided with a switchable partition, and when the partition is in a closed state, the plurality of partitions enclose an aquatic organism adaptation area; the plurality of the second containing tanks constitute an aquatic organism selection area; The variable spectrum lamp comprises a lampshade; a dimming knob is arranged outside the lampshade; The detachable bracket is arranged below the plurality of boxes; A plurality of box body slots are arranged above the detachable bracket, and a plurality of boxes are arranged on the plurality of box body slots respectively; a roller is arranged below the detachable bracket; The lens is arranged above the plurality of boxes; the camera is arranged above the lens; During measurement, each of the variable spectrum lamps emits light of a different spectrum, the partition is in an open state, and the aquatic organisms to be measured move from the aquatic organism adaptation area to the aquatic organism selection area, thereby completing the quantitative measurement of the aquatic organism light preference.

2. The device for quantitatively determining the light preference of aquatic organisms according to claim 1, characterized in that: The number of the boxes is six, and the boxes are rectangular boxes. The angle between two adjacent boxes is 60 degrees.

3. The device for quantitatively determining the light preference of aquatic organisms according to claim 1, characterized in that: The box body is made of white PVC board.

4. The device for quantitatively determining the light preference of aquatic organisms according to claim 1, characterized in that: The variable spectrum lamp comprises a lampshade and lamp beads arranged in the lampshade, the lampshade is a translucent acrylic cover, and the lamp beads are RGB lamp beads, LED lamp beads, IR LED lamp beads or 3030 packaged lamp beads with different wavelengths.

5. The device for quantitatively measuring the light preference of aquatic organisms according to claim 1, characterized in that: The detachable bracket is made of an aluminum alloy metal plate, and the roller is made of a plastic material.

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