Detection system for analyzing one or more particles

Abstract

A detection system for analyzing one or more particles, when a controller controls a drive device to position a sampling needle at a sample aspiration position, the sampling needle is in communication with a first fluid power source through a detection chamber, and the controller controls the first fluid power source to provide power to draw a sample through the sampling needle at the sample aspiration position; the controller controls the drive device to position the sampling needle at a waste position, the sampling needle is in communication with the first fluid power source through the detection chamber, and the controller controls the first fluid power source to provide power to expel a cleaning fluid through the sampling needle to clean the sampling needle. It can be seen that the present application can directly transport the sample to the detection chamber, without a "sample loop" for temporarily storing the sample, reducing the loss of particles; and the sample is aspirated and the liquid is discharged by the sampling needle, which simply and quickly realizes the cleaning of the sampling needle.

Description

Technical Field

[0001] This invention relates to the field of biochip detection technology, and more specifically to a detection system for analyzing one or more particles. Background Technology

[0002] Liquid-phase suspended chip technology is a cutting-edge biomolecular detection technology that integrates fluid dynamics, microparticle synthesis, biomolecular hybridization, and high-efficiency digital signal processing. Its principle involves integrating known biomolecules (DNA, RNA, peptides, proteins, etc.) onto the surface of one or more microparticles to form a probe array. This array captures one or more analytes from the sample and labels the analytes with one or more fluorescent substances (fluorescent dyes, fluorophores, or fluorescent microparticles), enabling detection using optical methods. Liquid-phase suspended chip technology offers significant advantages for biomolecular detection, including high precision, high throughput, high speed, and low cost, and is hailed as a novel biomolecular detection technology.

[0003] Traditional detection methods utilize flow cytometry, which measures internally or externally stained particles coupled with fluorescent dyes or fluorophores to analyze the optical characteristics of the particles themselves and the analyte, thereby revealing information about the target indicator. A similar product is the Luminex 200 from Luminex Corporation in the United States, which offers high sensitivity and specificity for biomolecular detection. However, flow cytometry involves several relatively sophisticated and expensive components, such as lasers, high-precision syringe pumps, photomultiplier tubes (PMTs), and avalanche photodiodes. While such systems offer superior performance, their cost is relatively high. Furthermore, flow cytometry requires a large amount of sheath fluid to operate properly, and the instruments are relatively large, heavy, fragile, and complex, requiring specialized technicians for installation.

[0004] For the reasons mentioned above, technicians have gradually noticed that fluorescence imaging technology can also be applied to biomolecular detection techniques. Such systems can classify cells or particles using images generated by light of different wavelengths. For example, patent CN101479603B by Wayne D. Ron et al. describes a system and method for performing measurements of one or more materials using fluorescence imaging technology, which, compared to systems used in flow cytometry, offers numerous advantages such as lower cost, simpler optical configuration, more stable mechanical properties, smaller size, higher detection sensitivity, and less sheath fluid usage. In a preferred form of this patent, such as... Figure 1As shown, its fluid handling subsystem includes a sample container 180, a sample loop 140, an imaging chamber 120, two valves (130 and 160), and a pump 170, etc. It requires the sample loop 140 to temporarily store the sample. The presence of the sample loop 140 increases the length of the pipe required to drive the sample to the imaging chamber, complicating the sample aspiration process. Furthermore, since some analyte particles remain on the pipe wall or in the pipe during the process of driving the sample into the imaging chamber, the longer pipe leads to greater particle loss, thus affecting the accuracy of the detection results. This patent also provides an alternative liquid path, such as... Figure 2 As shown, it saves costs by not having a sample loop 140, but it cannot clean the sample probe with a washing fluid. This results in the previous sample remaining in the sample probe after one sample is detected, thus interfering with the detection results. Summary of the Invention

[0005] This application provides a detection system for analyzing one or more particles, designed to be easy to clean and to avoid interference with the detection results.

[0006] According to a first aspect, one embodiment provides a detection system for analyzing one or more particles, comprising:

[0007] A detection chamber is a place for detecting one or more particles to be analyzed.

[0008] A first fluid power source used to power the flow of liquid in a fluid circuit;

[0009] A sampling needle is used to draw a sample using power provided by a first fluid power source, and the sampling needle is connected to the first fluid power source through the detection chamber;

[0010] The controller is used to control the first fluid power source to provide power to make the sampling needle draw up the sample; and to control the first fluid power source to provide power to discharge the cleaning fluid through the sampling needle to clean the sampling needle.

[0011] A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves.

[0012] The detection device is used to detect the optical signals of particles in the detection chamber.

[0013] According to a second aspect, one embodiment provides a detection system for analyzing one or more particles, comprising:

[0014] A sampling position is used to provide a sample to be analyzed, the sample containing one or more particles to be analyzed;

[0015] A detection chamber is a place for detecting one or more particles to be analyzed.

[0016] Wastewater discharge level, used to receive wastewater;

[0017] A first fluid power source used to power the flow of liquid in a fluid circuit;

[0018] A sampling needle is used to draw a sample at the sampling position by relying on the power provided by a first fluid power source, and the sampling needle is connected to the first fluid power source through the detection chamber;

[0019] A driving device for driving the sampling needle and / or the aspiration position to move so that the sampling needle is located at the aspiration position when aspiration is performed, and for driving the sampling needle and / or the waste liquid position to move so that the sampling needle is located at the waste liquid position when waste liquid is discharged;

[0020] The controller is used to control the action of the first fluid power source and control the drive device. When the controller controls the drive device to make the sampling needle position at the sampling position, the sampling needle is connected to the first fluid power source through the detection chamber, and the first fluid power source is controlled to provide power to make the sampling needle pick up the sample at the sampling position.

[0021] The controller is also used to control the drive device to position the sampling needle at the waste liquid level. The sampling needle is connected to the first fluid power source through the detection chamber. The controller controls the first fluid power source to provide power to discharge the cleaning liquid through the sampling needle to clean the sampling needle.

[0022] A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves.

[0023] The detection device is used to detect the optical signals of particles in the detection chamber.

[0024] According to a third aspect, one embodiment provides a detection system for analyzing one or more particles, comprising:

[0025] A detection chamber is a place for detecting one or more particles to be analyzed.

[0026] A first fluid power source is used to provide power for the flow of liquid in the liquid circuit, and the first fluid power source is connected to the outlet of the detection chamber;

[0027] A second fluid power source used to power the flow of liquid in a fluid circuit;

[0028] A sampling needle, used to draw samples using power provided by a first fluid power source;

[0029] The first gating device is used to select the connection, and the entrance to the detection chamber and the second fluid power source are both connected to the sampling needle through the first gating device;

[0030] The controller is used to control the first gating device to select, the first fluid power source to operate, and the second fluid power source to operate. The first gating device connects the sampling needle to the inlet of the detection chamber, the first fluid power source provides power to make the sampling needle draw up the sample, and the first or second fluid power source transports the sample drawn up in the liquid path to the detection chamber.

[0031] The controller is also used to connect the sampling needle and the second fluid power source through the first gating device, control the second fluid power source to provide power, and discharge the cleaning fluid through the sampling needle to clean the sampling needle.

[0032] A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves.

[0033] The detection device is used to detect the optical signals of particles in the detection chamber.

[0034] According to a fourth aspect, one embodiment provides a detection system for analyzing one or more particles, comprising:

[0035] A sampling position is used to provide a sample to be analyzed, the sample containing one or more particles to be analyzed;

[0036] A detection chamber is a place for detecting one or more particles to be analyzed.

[0037] Wastewater discharge level, used to receive wastewater;

[0038] A first fluid power source is used to provide power for the flow of liquid in the liquid circuit, and the first fluid power source is connected to the outlet of the detection chamber;

[0039] A second fluid power source used to power the flow of liquid in a fluid circuit;

[0040] A sampling needle is used to draw a sample using power provided by a first fluid power source at the sampling position;

[0041] A driving device for driving the sampling needle and / or the aspiration position to move so that the sampling needle is located at the aspiration position when aspiration is performed, and for driving the sampling needle and / or the waste liquid position to move so that the sampling needle is located at the waste liquid position when waste liquid is discharged;

[0042] The first gating device is used to select the connection, and the entrance to the detection chamber and the second fluid power source are both connected to the sampling needle through the first gating device;

[0043] The controller is used to control the second gate device, the first gate device, the operation of the first fluid power source, the operation of the second fluid power source, and the drive device. When the controller controls the drive device to position the sampling needle at the sampling position, the first gate device connects the sampling needle to the inlet of the detection chamber, the second gate device connects the first fluid power source to the outlet of the detection chamber, the first fluid power source provides power to make the sampling needle pick up the sample at the sampling position, and the first or second fluid power source transports the sample picked up in the liquid path to the detection chamber.

[0044] The controller is also used to control the drive device to position the sampling needle at the waste liquid level, connect the sampling needle and the second fluid power source through the first gating device, control the second fluid power source to provide power, and discharge the cleaning liquid through the sampling needle to clean the sampling needle.

[0045] A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves.

[0046] The detection device is used to detect the optical signals of particles in the detection chamber.

[0047] According to a fifth aspect, one embodiment provides a detection system for analyzing one or more particles, comprising:

[0048] A detection chamber is a place for detecting one or more particles to be analyzed.

[0049] A first fluid power source is used to provide power for the flow of liquid in the liquid circuit, and the first fluid power source is connected to the outlet of the detection chamber;

[0050] A second fluid power source used to power the flow of liquid in a fluid circuit;

[0051] A sampling needle, used to draw samples using power provided by a first fluid power source;

[0052] A three-way device having a first interface, a second interface, and a third interface, wherein the first interface, the second interface, and the third interface are interconnected; the first interface of the three-way device is connected to the entrance of the detection chamber, the second interface of the three-way device is connected to the sampling needle, and the third interface of the three-way device is connected to a second fluid power source;

[0053] The controller is used to control the first fluid power source to provide power so that the sampling needle can draw up the sample, and to control the first fluid power source or the second fluid power source to transport the sample drawn into the fluid path to the detection chamber.

[0054] The controller is also used to control the second fluid power source to provide power to discharge the cleaning fluid through the sampling needle to clean the sampling needle;

[0055] A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves.

[0056] The detection device is used to detect the optical signals of particles in the detection chamber.

[0057] According to the detection system for analyzing one or more particles according to the above embodiments, when the controller controls the drive device to position the sampling needle at the sampling position, the sampling needle is connected to the first fluid power source through the detection chamber. The first fluid power source is controlled to provide power so that the sampling needle can draw up the sample at the sampling position. When the controller controls the drive device to position the sampling needle at the waste liquid discharge position, the sampling needle is connected to the first fluid power source through the detection chamber. The first fluid power source is controlled to provide power to discharge the cleaning liquid through the sampling needle to clean the sampling needle. Therefore, the present invention can directly transport the sample to the detection chamber without the need for a "sample loop" for temporary sample storage, reducing particle loss. Moreover, using the sampling needle to draw up the sample and discharge the liquid provides a simple and quick way to clean the sampling needle. Attached Figure Description

[0058] Figure 1 This is a block diagram of a liquid circuit structure in an existing detection system;

[0059] Figure 2 This is a block diagram of another liquid circuit structure in an existing detection system;

[0060] Figure 3 This is a structural block diagram of an embodiment of the detection system for analyzing one or more particles provided by the present invention;

[0061] Figure 4 This is a structural block diagram of another embodiment of the detection system for analyzing one or more particles provided by the present invention;

[0062] Figure 5 A schematic diagram of a liquid circuit subsystem in a detection system for analyzing one or more particles provided by the present invention;

[0063] Figure 6 The present invention provides a structural block diagram of a liquid circuit subsystem in a detection system for analyzing one or more particles, as described in Embodiment 1.

[0064] Figure 7 The structural block diagram of the liquid circuit subsystem in the detection system for analyzing one or more particles provided by the present invention is shown in Embodiment 2.

[0065] Figure 8 The structural block diagram of the liquid circuit subsystem in the detection system for analyzing one or more particles provided by the present invention is shown in Embodiment 3.

[0066] Figure 9Structural block diagrams of embodiments four and eight of the liquid circuit subsystem provided by the present invention for analyzing one or more particles;

[0067] Figure 10 The structural block diagram of the liquid circuit subsystem in Embodiment 5 of the detection system for analyzing one or more particles provided by the present invention;

[0068] Figure 11 A structural block diagram of the liquid circuit subsystem in Embodiment Six of the detection system for analyzing one or more particles provided by the present invention;

[0069] Figure 12 Structural block diagrams of embodiments seven and nine of the liquid circuit subsystem provided by the present invention for analyzing one or more particles;

[0070] Figure 13 A structural block diagram of an embodiment of the optical path subsystem in the detection system for analyzing one or more particles provided by the present invention;

[0071] Figure 14 A flowchart of the detection method in the detection system for analyzing one or more particles provided by the present invention;

[0072] Figure 15 A structural block diagram of an embodiment of the second gating device in the detection system for analyzing one or more particles provided by the present invention;

[0073] Figure 16 A structural block diagram of another embodiment of the second gating device in the detection system for analyzing one or more particles provided by the present invention;

[0074] Figure 17 A schematic diagram of a detection system for analyzing one or more particles provided by the present invention, wherein the liquid circuit subsystem has two fluid power sources;

[0075] Figure 18 A structural block diagram of the liquid circuit subsystem in Embodiment 10 of the detection system for analyzing one or more particles provided by the present invention;

[0076] Figure 19 The structural block diagram of the liquid circuit subsystem in Embodiment Eleven of the detection system for analyzing one or more particles provided by the present invention;

[0077] Figure 20 In the detection system for analyzing one or more particles provided by the present invention, the liquid circuit subsystem includes a liquid circuit diagram of a three-way device. Detailed Implementation

[0078] The present invention will now be described in further detail with reference to specific embodiments and accompanying drawings.

[0079] exist Figure 1 In the existing technology shown, a sample needs to be drawn up and temporarily stored in a sample loop 140 using a combination of valves and pumps. Then, the valve state is changed to alter the pump's direction of action on the liquid, transporting the sample from the sample loop 140 to the imaging chamber 120. The presence of the sample loop 140 not only complicates the sample transport process to the imaging chamber but also lengthens the pipeline required to drive the sample to the imaging chamber. Since some analyte particles remain on the pipe wall or in the pipe during sample transport, a longer pipeline leads to greater particle loss, thus affecting the accuracy of the detection results. Figure 2 In the prior art shown, pump 170 is a one-way pump, and the tube or probe between valve 190 and sample container 180 cannot be cleaned. However, this invention, by optimizing the liquid path structure, not only allows the sample to directly enter the detection chamber without temporary storage, but also facilitates probe cleaning. Specific details are provided below.

[0080] The detection system for analyzing one or more particles provided by this invention is applicable to liquid phase suspension chip detectors (also known as liquid phase biochip detectors, or liquid phase chip detectors), and of course, it can also be applied to other instruments that require the analysis of one or more particles. For example... Figure 3 As shown, the detection system includes: a controller 1, an optical path subsystem 2, a liquid path subsystem 3, and a drive subsystem 5. The controller 1 controls the optical path subsystem 2, the liquid path subsystem 3, and the drive subsystem 5 to detect one or more particles. Please refer to [further details omitted]. Figure 5 The liquid circuit subsystem includes at least: a sampling needle a, a sample aspiration position b, a waste liquid discharge position c, a detection chamber 33, and a first fluid power source 31. The sampling needle a is connected to the first fluid power source 31 through the detection chamber 33.

[0081] The sampling position b refers to the location where the sampling needle draws up the sample. In one embodiment, this position can be a fixed facility, such as a recessed slot, for placing the sample container 36 to provide the sample to be analyzed. Alternatively, the sampling position b can also be a container itself, used to hold the sample to be analyzed. In some embodiments, the sampling position b can also be a coordinate location, which is moved by the sampling needle and / or the sample container to collect the sample when it is necessary to draw up the sample.

[0082] The sample container 36 contains the sample to be analyzed. Specifically, the sample is stored in liquid form in the sample container 36 and contains one or more particles to be analyzed. These particles can be particles bound to the analyte and labeled with a fluorescent substance. Different analytes typically result in different particle types. This invention can detect one or more analytes. The analyte can be a biomolecule, etc. Particles can be encoded by particle shape, particle pattern (e.g., arbitrary patterns can be etched onto the particle surface), particle size, fluorescence spectrum, or Raman spectrum. The particle size can range from nanometers to micrometers and can be microparticles of various shapes; for example, particles can be microspheres with a diameter ranging from nanometers to micrometers. The analyte is usually associated with a fluorescent substance. For example, the fluorescent substance is used to identify the presence or quantity of the analyte. In some embodiments, a fluorescent substance is associated with one analyte, and its specific fluorescence identifies the corresponding analyte. The fluorescent substance is typically a fluorescent dye, quantum dot, or upconversion luminescent material, etc. The fluorescent substance emits fluorescence when excited by light.

[0083] The detection chamber 33 provides a detection site for one or more particles to be analyzed, for example, providing space for a single-layer arrangement of particles in the sample, so that the optical subsystem 2 can detect the particles within the detection chamber 33. Typically, the detection of particles by the optical subsystem 2 involves imaging them, and then identifying brightness, quantity, etc., based on the resulting image. Therefore, the detection chamber 33 is usually an imaging chamber. The sample particles within the detection chamber 33 are arranged in a single layer under the influence of gravity, magnetism, or electric field, forming a detection plane. The controller 1 can control the first fluid power source 31 to cause the particles in the sample to arrange in a single layer within the detection chamber 33 under the influence of gravity, or it can generate magnetic or electric field forces through a specific device to constrain the particles within the detection chamber 33, ensuring their single-layer arrangement. The detection chamber 33 is at least transparent from the front, allowing the optical subsystem 2 to detect particles through the front of the detection chamber 33, for example, through imaging detection. The back of the detection chamber 33 can be transparent or opaque.

[0084] The waste liquid discharge level 'c' refers to the location where waste liquid is discharged. In one embodiment, this location can be a fixed facility, such as a recessed trough, for housing the waste liquid recovery device 38 to receive waste liquid. Alternatively, the waste liquid discharge level 'c' can also be a container itself used to receive waste liquid. Or, for example, the waste liquid discharge level 'c' can also be a container itself used to hold the sample to be analyzed. In a specific embodiment, the waste liquid can be discharged through a sampling needle. In some embodiments, the waste liquid discharge level 'c' can also be a coordinate position, where the sampling needle and / or waste liquid container are moved to achieve waste liquid discharge when it is necessary. The waste liquid recovery device 38 can be a section of pipe or any other suitable container configuration capable of receiving waste liquid. Receiving waste liquid can specifically mean containing waste liquid or receiving waste liquid and then discharging it. The waste liquid can be a sample that has been tested or a cleaning solution. When the cleaning solution is discharged through the sampling needle, the sampling needle is also cleaned.

[0085] The first fluid power source 31 provides power for the flow of liquid in the liquid path. It operates according to the control of the controller 1, thereby providing power for the flow of liquid in the liquid path, such as drawing samples or cleaning solutions into and out of the liquid path. The first fluid power source 31 is a device capable of driving liquid flow in a pipeline, such as various types of pumps or combinations of multiple pumps, or combinations of multiple pumps connected by pipes, valves, etc. The controller 1 is electrically connected to the first fluid power source 31 to control its operation. The liquid path subsystem is essentially sealed so that the first fluid power source 31 can draw liquid through the liquid path, drive the liquid to the corresponding location, and discharge the liquid.

[0086] The sampling needle a is used to draw up a sample at the sampling position using the power provided by the first fluid power source 31.

[0087] The drive subsystem 5 includes a drive unit (not shown in the figure). The controller 1 is electrically connected to the drive unit to control the drive unit to perform drive.

[0088] The driving device is used to move the sampling needle a and / or the aspiration position b so that the sampling needle a is positioned at the aspiration position during aspiration, facilitating sample aspiration from the sample container 36 at the aspiration position. Specifically, the sampling needle a is positioned at a location adapted to the sample container 36, where it can aspirate the sample. The driving device can achieve this positioning of the sampling needle a at the aspiration position in three ways. The first way: the aspiration position b does not move, and the driving device only moves the sampling needle a to achieve this positioning. The second way: the sampling needle a does not move, and the driving device only moves the aspiration position b to achieve this positioning. The third of the three methods: The driving device drives the sampling needle a and the aspiration position b to move, so that the sampling needle a is located at the aspiration position when aspirating; for example, the driving device drives the aspiration position to move below the sampling needle a, drives the sampling needle a to move downward, so that the aspiration end of the sampling needle a extends into the sample container 36 on the aspiration position b, so that the inlet of the detection chamber 33 is connected to the sample container 36, and then the first fluid power source 31 generates power to draw the sample into the liquid path.

[0089] The drive unit is also used to move the sampling needle a and / or the waste liquid level c, so that the sampling needle a is located at the waste liquid level during waste liquid discharge, facilitating the discharge of waste liquid to the waste liquid recovery device 38 at the waste liquid level. Specifically, the sampling needle a being located at the waste liquid level is positioned to match the waste liquid recovery device 38, where it can discharge waste liquid into the waste liquid recovery device 38. The drive unit also has three methods to ensure that the sampling needle a is located at the waste liquid level during waste liquid discharge. The first method: the waste liquid level c does not move, and the drive unit only drives the sampling needle a to move, so that the sampling needle a is located at the waste liquid level during waste liquid discharge. The second method: the sampling needle a does not move, and the drive unit only drives the waste liquid level c to move, so that the sampling needle a is located at the waste liquid level during waste liquid discharge. The third of the three methods: The driving device drives the sampling needle a and the waste liquid level c to move, so that the sampling needle a is located at the waste liquid level when the waste liquid is discharged; for example, the driving device drives the waste liquid level to move below the sampling needle a, drives the sampling needle a to move downward, so that the sampling end of the sampling needle a extends into the waste liquid recovery device 38 on the waste liquid level, and then the first fluid power source 31 generates power to discharge the waste liquid from the sampling needle a.

[0090] In this system, the sampling position b and the waste liquid discharge position c can be different stations. For example, the drive subsystem 5 also includes a tray. One position on the tray is used to place the sample container 36 (sampling position b), and the other position is used to place the waste liquid recovery device 38 (waste liquid discharge position c). The drive device moves the sampling needle a and / or the tray so that the sampling needle a is located at the sampling position during sampling and at the waste liquid discharge position during waste liquid discharge. Of course, the sampling position b and the waste liquid discharge position c can also be the same station. For example, the drive subsystem 5 also includes a tray. One position on the tray is used to place both the sample container 36 and the waste liquid recovery device 38. When the sample container 36 is placed at this position, it is the sampling position b, and when the waste liquid recovery device 38 is placed at this position, it is the waste liquid discharge position c. In practice, the sample container 36 can also be used as the waste liquid recovery device 38 to collect waste liquid after sampling.

[0091] like Figure 13 As shown, the optical path subsystem 2 includes a light source (not shown) and a detection device 21. The controller 1 is electrically connected to the light source and the detection device 21, and controls the optical path subsystem 2 by controlling the light source and the detection device 21. The light source is used to irradiate the particles f in the detection chamber 33, causing the particles f to emit light signals related to the characteristics of the particles themselves, such as fluorescence. The detection device 21 is used to detect the light signals of the particles f in the detection chamber 33. The controller 1 controls the liquid path subsystem 3, the drive subsystem 5, the light source, and the detection device 21 to detect the light signals of the particles f in the detection chamber 33. Since the particles possess fluorescent substances, the fluorescent substances are excited under the irradiation of the light source, thereby emitting fluorescence. Furthermore, the detection device 21 detects the intensity (luminescence intensity) of the fluorescence of the particles f in the detection chamber 33. The detection device 21 can detect the fluorescence of particles f in the detection chamber 33 when the light source irradiates the particles f, or it can detect the fluorescence of particles f in the detection chamber 33 after the light source irradiates the particles f (for example, upconversion luminescent materials have a long fluorescence lifetime, and the particles still emit fluorescence for a period of time after the light source stops irradiating). For example, it can obtain the light intensity by imaging and detecting particles arranged in a single layer (e.g., detecting the pixel value of particle pixels); the present invention is not limited to this.

[0092] Controller 1 controls the optical subsystem 2, the liquid subsystem 3, and the drive subsystem 5 to detect one or more particles, as follows: Figure 14 As shown, it includes the following steps:

[0093] Pretreatment step S1: Controller 1 controls the first fluid power source to draw in cleaning fluid through the liquid path.

[0094] In the sampling step S2, controller 1 controls the operation of the first fluid power source 31 and the drive device. When controller 1 controls the drive device to position the sampling needle a at the sampling position, the sampling needle a is connected to the first fluid power source 31 through the detection chamber 33. The first fluid power source 31 provides power to allow the sampling needle a to draw the sample at the sampling position b and transport the drawn sample to the detection chamber 33. In this embodiment, the sample container 36 placed at the sampling position b is used as an example. That is, the first fluid power source 31 provides power to allow the sampling needle a to draw the sample from the sample container 36 placed at the sampling position b and transport the drawn sample to the detection chamber 33. The sample drawn by the sampling needle a is the sample to be tested. It can be seen that the sample drawn by the sampling needle a can be directly drawn into the detection chamber 33 without the need for a sample loop for temporary sample storage. The liquid path traversed by the sample is short, thus minimizing particle loss. When the sampling needle a is in the sampling position, there are two situations in which the sampling needle a is connected to the first fluid power source 31 through the detection chamber 33. In one embodiment without the first gating device, the sampling needle a is connected to the detection chamber 33 and the first fluid power source 31 in sequence. In the other embodiment with the first gating device, the first gating device is controlled to make the sampling needle a connect to the first fluid power source 31 through the detection chamber 33.

[0095] In detection step S3, controller 1 controls the optical path subsystem 2 to detect the sample to be tested in the detection chamber.

[0096] In cleaning step S4, after the test is completed, the sampling needle a is connected to the first fluid power source 31 through the test chamber 33. The controller 1 controls the first fluid power source 31 to provide power to discharge the cleaning liquid through the sampling needle a to clean the sampling needle a. Before discharging the cleaning liquid through the sampling needle a to clean the sampling needle a, the controller 1 also controls the drive device to position the sampling needle a at the waste liquid level c, so that the cleaning liquid for cleaning the sampling needle a can be discharged to the waste liquid recovery device at the waste liquid level c. The action of controlling the drive device to position the sampling needle a at the waste liquid level c can be performed after sampling to ensure that the sampling needle a is at the waste liquid level c when cleaning the sampling needle. For example, the controller 1 controls the drive device to position the sampling needle a at the waste liquid level c immediately after sampling, or, during the process of transporting the sample to the test chamber (at which time the sample has been sampled), the controller 1 controls the drive device to position the sampling needle a at the waste liquid level c, or, during the test, the controller 1 controls the drive device to position the sampling needle a at the waste liquid level c, or, after the test is completed, the controller 1 controls the drive device to position the sampling needle a at the waste liquid level c. In this embodiment, the waste liquid recovery device 38 is placed at the waste liquid discharge level c as an example. Specifically, the cleaning liquid is discharged through the sampling needle a to the waste liquid recovery device 38 to clean the sampling needle a. Specifically, at least a section of the cleaning liquid from the first fluid power source 31 to the sampling needle a is discharged through the sampling needle a to the waste liquid recovery device 38 to clean the sampling needle a. Since the same sampling needle a is used for both sample aspiration and cleaning liquid discharge, the cleaning liquid discharge effectively cleans the sampling needle a, making the cleaning process simple and efficient. The sampling needle a can be a tube with a needle tip at one end, a needle tip, or a tube, etc., as long as it can aspirate and discharge liquid; this invention does not limit this. The sampling needle a is located at the waste liquid discharge level c. There are two scenarios where the sampling needle a is connected to the first fluid power source 31 through the detection chamber 33: one is in an embodiment without a first gating device, where the sampling needle a is sequentially connected to the detection chamber 33 and the first fluid power source 31; the other is in an embodiment with a first gating device, where the first gating device is controlled to connect the sampling needle a to the first fluid power source 31 through the detection chamber 33.

[0097] Furthermore, Figure 5In the schematic diagram shown, the first fluid power source 31 is connected to the outlet of the detection chamber 33. It can be directly connected to the outlet of the detection chamber 33, or it can be connected to the outlet of the detection chamber 33 through the first pipe g1. This invention is illustrated by taking the connection of the first fluid power source 31 to the outlet of the detection chamber 33 through the first pipe g1 as an example. The sampling needle a is connected to the inlet of the detection chamber 33. The detection chamber 33 and the sampling needle a can be cleaned at once during the drainage process. That is, the controller 1 controls the first fluid power source 31 to provide power, and the cleaning liquid from the first fluid power source 31 to at least one section of the sampling needle a is discharged through the sampling needle a to the waste liquid recovery device 38 to clean the sampling needle a. This includes: the controller 1 controls the first fluid power source 31 to provide power, and the cleaning liquid from the first fluid power source 31 to at least one section of the first pipe g1 (the cleaning liquid in the first fluid power source 31 and / or the cleaning liquid in the first pipe g1), the sample to be tested in the detection chamber, and the liquid from the detection chamber to the sampling needle are discharged through the sampling needle to the waste liquid recovery device to discharge waste liquid and clean the sampling needle. Figure 5 In this process, at least one section of the cleaning fluid from the first fluid power source 31 to the first pipe g1 is closest to the power source. In front of it (the front-to-back relationship is determined by the direction of liquid flow), there is a sample. The first fluid power source 31 pushes this section of cleaning fluid through the detection chamber 33 to clean the detection chamber 33, and then discharges it from the sampling needle a to clean the sampling needle a. It can be seen that the first fluid power source 31 can complete the cleaning of the detection chamber 33 and the sampling needle a simply and conveniently by continuously pumping out the liquid. Since the sample is in front of this section of cleaning fluid, the cleaning fluid flows through the detection chamber 33 and the sampling needle a in sequence, which also pushes the liquid (sample, cleaning fluid, etc.) in front of this section of cleaning fluid to be discharged, realizing the discharge of waste liquid and the cleaning of the liquid path, which is highly efficient.

[0098] As can be seen from the above, sample loading and waste liquid discharge are performed automatically. Of course, users can also operate them manually. For example, without the need for a sample suction position, waste liquid discharge position, and drive device, when loading the sample, the user can manually place the sample container 36 under the sampling needle a, and manually place the waste liquid recovery device under the sampling needle a, etc.

[0099] In the manual mode, controller 1 controls the optical subsystem 2, liquid subsystem 3, and drive subsystem 5 to detect one or more particles. For example, the user manually places the sample container 36 below the sampling needle a, ensuring the needle a is below the liquid surface. Then, controller 1 controls the first fluid power source 31 to provide power, causing the sampling needle a to draw the sample from the sample container 36 and transport it to the detection chamber 33. The optical subsystem 2 then detects the sample in the detection chamber 33. After sampling, the user manually places the waste liquid recovery device below the sampling needle a. After detection, controller 1 controls the first fluid power source 31 to provide power to discharge the cleaning solution through the sampling needle a to the waste liquid recovery device to clean the needle. "After sampling" typically refers to the sampling needle a drawing up a sufficient amount of sample for detection. Therefore, the manual mode primarily involves the user manually replacing the drive device and does not require a sampling position or a waste liquid discharge position. Other processes are the same as in the automated mode and will not be elaborated further.

[0100] Figure 5 This is a schematic diagram of a liquid circuit subsystem. Specific liquid circuit subsystems can take many forms, such as... Figure 6-12 As shown, Figure 6-12 The arrows in the diagram indicate the direction of liquid flow. A single arrow indicates unidirectional flow; a double arrow indicates bidirectional flow, meaning the liquid can flow in one direction at one moment and in the other at another. The following sections will explain... Figure 6-12 The seven liquid circuit subsystems shown are illustrated as seven examples.

[0101] Example 1 ( Figure 6 The embodiment of the liquid circuit subsystem shown is described below. In this embodiment, in... Figure 5 Based on the liquid circuit subsystem shown, a first gate device 34 is added. In this embodiment, the first gate device 34 has an outlet 1, a first inlet 2, and a second inlet 3. The first gate device 34 selects the detection chamber 33, the sampling needle a, and the second pipe g2. Specifically, it is used to connect the outlet 1 to the first inlet 2 and, after switching, to connect the outlet 1 to the second inlet 3. The outlet 1 of the first gate device 34 is directly connected to the inlet of the detection chamber 33 or connected to the inlet of the detection chamber 33 through a pipe. The first inlet 2 of the first gate device 34 is connected to the sampling needle a, and the second inlet 3 of the first gate device 34 is connected to the cleaning fluid container 37 for providing cleaning fluid through the second pipe g2. The first fluid power source 31 is connected to the outlet of the detection chamber 33 through the first pipe g1. The connections in the liquid circuit of this invention can be direct connections or connections through pipes. The cleaning fluid container 37 stores cleaning fluid. The cleaning solution plays a driving and washing role in the liquid circuit subsystem. Any liquid that can play a driving and washing role can be used, such as phosphate buffer, carbonate buffer, ammonium buffer, deionized water, purified water, etc. This invention does not limit the scope of the invention.

[0102] The first gate device 34, based on the control of the controller 1, selects at least one of the sampling needle a and the second pipe g2 to communicate with the inlet of the detection chamber 33. The first gate device 34 may include one or more pipes and one or more valves. The valves in this invention can be any suitable valve known in the art. This embodiment is illustrated by taking the first gate device 34 including a first valve J as an example. The first valve J includes three connectors for liquid inflow and outflow, which respectively serve as the outlet 1, the first inlet 2, and the second inlet 3 of the first gate device 34. Connector 1 of the first valve J is connected to the inlet of the detection chamber 33, connector 2 of the first valve J is connected to one end of the sampling needle a, and connector 3 of the first valve J is connected to the second pipe g2. The second pipe g2 can be any type of pipe, such as a probe, as long as it can draw liquid; this invention is not limited thereto.

[0103] In this embodiment, controller 1 controls optical subsystem 2, liquid subsystem 3, and drive subsystem 5 to detect one or more particles, including the following steps:

[0104] Pre-processing step S1.1: Controller 1 controls the first gate device 34 to connect the inlet of the detection chamber 33 to the second pipe g2, and controls the first fluid power source 31 to provide power so that the second pipe g2 draws cleaning fluid from the cleaning fluid container 37 until the entire fluid path is filled with cleaning fluid. For example, the entire... Figure 6 The liquid path shown is sealed. The first fluid power source 31 includes a pump, which is in a suction operating state. The cleaning fluid is drawn into the liquid path, and the pump continues to operate to fill the entire liquid path with cleaning fluid. At this time, the pump also has the ability to continue suction for subsequent sample suction. Filling the entire liquid path with cleaning fluid before sample suction can prevent air bubbles from appearing in the liquid path and avoid interference with the imaging of the detection chamber 33. Of course, filling the entire liquid path with cleaning fluid before sample suction is not necessary. Regardless of whether the entire liquid path is filled with cleaning fluid, the controller 1 controls the first gate device 34 to connect the inlet of the detection chamber 33 to the second pipe g2, and controls the first fluid power source 31 to provide power so that the second pipe g2 draws cleaning fluid from the cleaning fluid container 37. The cleaning fluid drawn this time is used for subsequent cleaning, and the amount of cleaning fluid drawn is sufficient for cleaning after particle detection.

[0105] In the sampling step S2.1, controller 1 controls the drive device to position the sampling needle a at the sampling position, and controller 1 controls the first gate device 34 to connect the inlet of the detection chamber 33 with the sampling needle a. These two actions can be performed simultaneously or in any order. Thus, the first fluid power source 31, the detection chamber 33, the sampling needle a, and the sample container 36 are connected. The first fluid power source 31 then provides power to allow the sampling needle a to draw the sample from the sample container 36 placed at the sampling position b and transport the drawn sample to the detection chamber 33. The amount of sample drawn depends on the detection requirements. In this invention, the sample enters the detection chamber 33 directly from the sample container 36, without the need for temporary storage in the sample loop of the prior art. Therefore, the process is not only simple and efficient, but also results in less particle loss during pipeline operation.

[0106] The particles in the sample can be arranged in a single layer within the detection chamber 33 by gravity, or they can be constrained into a single layer by a particle constraint device. This embodiment will use the latter as an example. Figure 4 and Figure 13 As shown, in this embodiment, the detection system further includes a particle confinement device 4, and the controller 1 is electrically connected to the particle confinement device 4. The particles are magnetic particles f that are bound to the analyte and have fluorescent material markings. The particle confinement device 4, under the control of the controller 1, uses magnetic force to confine the particles f in the detection chamber 33 into a single layer, thus fixing the particles f in the detection chamber 33. The particle confinement device 4 is movable. The controller 1 controls the particle confinement device 4 to move to the bottom of the detection chamber 33, whereby the particle confinement device 4 uses magnetic force to confine and fix the particles f in the detection chamber 33. When the controller 1 controls the particle confinement device 4 to move away, the particles f are no longer bound by the magnetic force. The particle confinement device 4 includes a magnet and a moving device. The moving device is used to drive the magnet to move to the back of the detection chamber 33 and drive the magnet to move away. The magnet can be various types of magnets, such as permanent magnets, electromagnets, etc. Of course, in other embodiments, the particle confinement device 4 may not confine the particles f by magnetic force, but by electric field force. For example, the particle confinement device 4 first charges the particles f, and then confines the particles f in the detection chamber 33 into a single layer by electric field force, and fixes the particles f in the detection chamber 33 by electric field force.

[0107] In this step, after the sample is drawn, there are four ways to transport the drawn sample to the testing chamber 33. These are described below.

[0108] In method one, controller 1 controls the first selection device 34 to connect the inlet of detection chamber 33 to the second pipe g2, that is, the inlet of detection chamber 33 to the cleaning fluid container 37, and controls the first fluid power source 31 to aspirate the cleaning fluid. Thus, a liquid flow is formed in the liquid path with the cleaning fluid aspirated in step S1.1 in front, the sample aspirated in this step in the middle, and the cleaning fluid aspirated in this step behind. This ensures that the sample in the liquid path is surrounded by cleaning fluid, preventing the formation of air bubbles. Controller 1 controls the first fluid power source 31 to transport this liquid flow. First, the cleaning fluid in the liquid flow passes through detection chamber 33 (which also serves a cleaning function if the detection chamber 33 is not clean). Before the sample enters detection chamber 33, controller 1 controls the moving device to drive the magnet to move to the back of detection chamber 33, generating a magnetic field. The first fluid power source 31 continues to draw in liquid, and the sample enters the detection chamber 33. The particles f in the sample are arranged in a single layer and fixed under the influence of the magnetic field. At this point, the first fluid power source 31 can stop working. Preferably, the first fluid power source 31 continues to draw in liquid before stopping, so that some of the cleaning liquid after the sample enters the detection chamber 33, completing the rinsing of the particles f (the sample contains other impurities besides particles f; the cleaning liquid flowing through the detection chamber 33 carries away the impurities, while the particles f are fixed by the magnetic force and will not be carried away). Since the capacity, length, cross-sectional area, etc., of each component and pipe of the liquid circuit subsystem are known, and the flow rate of the liquid drawn in (pumped in) and pumped out (pumped out) by the first fluid power source 31 is known, controlling the working speed and working time of the first fluid power source 31 can deliver the liquid to the desired location.

[0109] In method two, controller 1 controls the first fluid power source 31 to transport the sample, such that a portion of the sample is located to the left of the outlet of detection chamber 33 (within the first pipe g1 and / or the first fluid power source 31), a portion of the sample is located between the outlet and inlet of detection chamber 33 (i.e., detection chamber 33 is filled with sample), and another portion of the sample is located to the right of detection chamber 33 (e.g., within the pipe between sampling needle a and / or sampling needle a and the inlet of detection chamber 33). In other words, controller 1 controls the first fluid power source to transport the fluid flow formed by the cleaning liquid and the sample collected in step S1.1, such that the cleaning liquid collected in step S1.1 and a portion of the sample collected in step S2.1 flow through detection chamber 33, a portion of the sample collected in step S2.1 fills detection chamber 33, and another portion of the sample collected in step S2.1 is located to the right of detection chamber 33 (e.g., within the pipe between sampling needle a and / or sampling needle a and the inlet of detection chamber 33). Similarly, before the sample enters detection chamber 33, controller 1 controls the moving device to drive the magnet to move to the back of detection chamber 33, generating a magnetic field. After the sample enters the detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the influence of the magnetic field. Thus, compared to method one, there is no need to continue aspirating the cleaning solution after the sample, and the detection chamber 33 is filled with liquid, preventing the generation of air bubbles.

[0110] Method 3: Controller 1 controls the first fluid power source 31 to transport the sample, placing it in the detection chamber 33. A portion of the cleaning fluid flowing through the detection chamber 33 is then pumped out by the first fluid power source 31 in the reverse direction to prevent air bubbles from forming in the detection chamber 33. In other words, controller 1 controls the first fluid power source 31 to transport the fluid flow formed by the cleaning fluid collected in step S1.1 and the sample collected in this step, ensuring the cleaning fluid flows through the detection chamber 33 and the sample is located in the detection chamber 33. Then, the first fluid power source 31 reverses the flow (i.e., pumps it out) to prevent air bubbles from forming in the detection chamber 33. Similarly, before the sample enters the detection chamber 33, controller 1 controls the moving device to drive the magnet to the back of the detection chamber 33, generating a magnetic field. After the sample enters the detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the influence of the magnetic field.

[0111] Method 4: Controller 1 controls the first fluid power source 31 to transport the sample, positioning it in the detection chamber 33. Similarly, before the sample enters the detection chamber 33, controller 1 controls a moving device to drive a magnet to the back of the detection chamber 33, generating a magnetic field. After the sample enters the detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the influence of the magnetic field.

[0112] In detection step S3.1, controller 1 controls detection device 21 to detect the optical signal of particle f in detection chamber 33. For example... Figure 13As shown, the optical path subsystem 2 also includes a filter switching device 22. The filter switching device 22 allows light of different wavelengths to pass through. It includes multiple imaging filters 221, one of which is positioned in the optical path between the detection chamber 33 and the detection device 21, thus allowing light of one wavelength to enter the detection device 21. By switching the imaging filters in the optical path, light of different wavelengths can enter the detection device separately. For example, the filter switching device 22 can be a filter wheel, including a wheel and multiple imaging filters arranged in a circular array on the wheel, one of which is located in the optical path between the detection chamber 33 and the detection device. The imaging filters are used to filter the fluorescence of particle f, for example, allowing only one type of fluorescence to pass through, thereby detecting one fluorescence signal; the rotation of the wheel to switch the imaging filter 221 allows the detection of another fluorescence signal.

[0113] Controller 1 turns on the light source to irradiate particle f, exciting the fluorescent material coupled inside or on the surface of particle f, causing it to emit fluorescence. When the light source is turned on, or after the light source irradiates particle f in the detection chamber, controller 1 activates detection device 21 to detect the fluorescence of particle f in detection chamber 33. Detection can be achieved by taking a picture of particle f in detection chamber 33, obtaining an image of particle f. Due to the effect of imaging filter 221, the light emitted by the light source is filtered out, resulting in a high-quality image. After obtaining the image of particle f, controller 1 can perform image processing, etc., to obtain the fluorescence intensity of particle f, thereby determining the type of particle f and the amount of the corresponding analyte bound to the particle f. Based on the detection results of all particles f in the image, the content or concentration of one or more analytes in the sample can be obtained.

[0114] The detection device 21 may include at least one of the following: a single-point photodetector (photodetector), a linear array of photodetectors, and a surface array of photodetectors. The surface array of photodetectors may preferably be a camera. Furthermore, the detection device 21 may include one or multiple photodetectors of each type.

[0115] In cleaning step S4.1, after the detection is completed, the controller 1 closes the particle confinement device 4 or controls the particle confinement device 4 to move away from the detection chamber 33, so that the particles in the detection chamber 33 are not confined by the particle confinement device 4, facilitating particle discharge. After sampling, this embodiment takes the completion of detection as an example. The controller 1 controls the drive device to position the sampling needle a at the waste liquid discharge position, and controls the first gate device 34 to switch, so that the inlet of the detection chamber 33 is connected to the sampling needle a. That is, the sampling needle a is connected to the first fluid power source 31 through the detection chamber 33, and the first fluid power source 31 is controlled to provide power, so that the cleaning liquid from the first fluid power source 31 to at least one section of the first pipeline g1, the sample to be tested in the detection chamber 33, and the liquid from the detection chamber 33 to the sampling needle a are discharged through the sampling needle a to the waste liquid recovery device 38 to discharge waste liquid and clean the detection chamber 33 and the sampling needle a. The controller 1 controls the drive device to position the sampling needle a at the waste liquid discharge position, and the controller 1 controls the first gate device 34 to connect the inlet of the detection chamber 33 to the sampling needle a. These two actions are not sequential and can be performed simultaneously.

[0116] Since the cleaning fluid drawn in step S1.1 flows from the first fluid power source 31 to the first pipe g1 (this cleaning fluid can be in the first fluid power source 31 and / or in the first pipe g1), when this section of cleaning fluid is pumped out, the cleaning fluid will flush the detection chamber 33. After the sample before the cleaning fluid is discharged into the waste liquid recovery device 38, the cleaning fluid is discharged, thereby flushing the sampling needle a. Thus, the liquid path that the sample passes through is cleaned by the cleaning fluid. It can be seen that... Figure 6 The liquid circuit subsystem shown not only allows for one-step sample loading but also cleans particles within the detection chamber 33; simultaneously, it cleans both the detection chamber 33 and the sampling needle a, achieving sample loading and cleaning in one step. Therefore, Figure 6 The liquid circuit subsystem shown has a simple and efficient process for sample loading, sample discharge, and post-discharge cleaning, and does not require a sample loop for temporary sample storage.

[0117] Example 2 ( Figure 7 The embodiment of the liquid circuit subsystem shown is described below. In this embodiment, in... Figure 5 Based on the liquid circuit subsystem, a second gate device 32 is added. The first fluid power source 31 is connected to the outlet of the detection chamber 33 via the first pipe g1 and the second gate device 32. The second gate device 32 is also connected to the cleaning fluid container 37' for providing cleaning fluid via the third pipe g3. The third pipe g3 can be directly connected to the cleaning fluid container 37', or it can be inserted below the liquid surface of the cleaning fluid container 37' when liquid aspiration is required, like a sampling needle. The third pipe g3 can be any type of tube, such as a probe, as long as it can aspirate liquid; this invention is not limited thereto.

[0118] In this embodiment, the second selection device 32 is used to select the first fluid power source 31, the detection chamber 33, and the third pipeline g3. Specifically, it is used to connect at least one of the third pipeline g3 and the detection chamber 33 to the first fluid power source 31. The second selection device 32 may be composed of a valve, such as... Figure 15 As shown, the second gate device 32 includes a second valve K, which includes connectors 1, 2, and 3 for liquid inflow and outflow. A first fluid power source 31, such as a pump, is connected to connector 1 of the second valve K via a first pipe g1. Connector 2 of the second valve K is connected to one end of a third pipe g3, the other end of which is used to draw liquid from a cleaning fluid container 37'. Connector 3 of the second valve K is connected to the outlet of the detection chamber 33. Controller 1 controls the connection of connector 1 and connector 2 of the second valve K, and when connector 3 is closed, the third pipe g3 is connected to the first fluid power source 31. Controller 1 controls the connection of connector 1 and connector 3 of the second valve K, and when connector 2 is closed, the detection chamber 33 is connected to the first fluid power source 31. The second gate device 32 can also consist of valves and pipes, such as... Figure 16 As shown, the second gate device 32 includes a third valve L, a fourth valve (not shown), and a pipe. The first fluid power source 31, such as a pump, has two ports for pumping in and out liquid. One port is connected to the outlet of the detection chamber 33 through a first pipe, and the other port is connected to the outlet of the third valve L through a pipe. The inlet of the third valve L is connected to the third pipe. The fourth valve can be installed on the first pipe or on the liquid path between the detection chamber 33 and the sampling needle a. Thus, when the third valve L is closed and the fourth valve is open, the pump 31 can draw in and drain liquid through the liquid path formed by the detection chamber 33 and the sampling needle a. When the fourth valve is closed and the third valve L is open, the pump 31 can draw in liquid from the cleaning liquid container 37'. Of course, the second gate device 32 can also be other structures, such as multiple pipes and valves, as long as the first fluid power source 31 can draw in cleaning liquid from the cleaning liquid container 37' and the first fluid power source 31 can provide power for sampling and draining liquid after switching.

[0119] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0120] In pretreatment step S1.2, controller 1 controls the second gating device 32 to connect the first fluid power source 31 to the third pipe g3, and controls the first fluid power source 31 to provide power so that the third pipe g3 draws cleaning fluid from the cleaning fluid container 37'. The amount of cleaning fluid drawn is sufficient for cleaning after particle detection. For example, the entire... Figure 7The liquid path shown is sealed. The first fluid power source 31 includes a pump, which is in a suction operating state. The cleaning fluid is drawn into the liquid path and located within the pump 31 and / or the first pipe g1. Similarly, the entire liquid path can be pre-filled with cleaning fluid before drawing it in. The cleaning fluid drawn in this step is typically used for cleaning after particle detection; therefore, this step can be performed before cleaning step S4.2.

[0121] In sampling step S2.2, controller 1 controls the second gate device 32 to switch the outlet of the detection chamber 33 to be connected to the first fluid power source 31. Controller 1 controls the drive device to position the sampling needle a at the sampling position. Thus, the first fluid power source 31, the detection chamber 33, the sampling needle a, and the sample container 36 are connected. The first fluid power source 31 is then controlled to provide power so that the sampling needle a draws the sample from the sample container 36 placed at the sampling position b and transports the drawn sample to the detection chamber 33. The amount of sample drawn depends on the detection requirements. In this invention, the sample enters the detection chamber 33 directly from the sample container 36, without the need for temporary storage in the sample loop of the prior art. Therefore, the process is not only simple and efficient, but also results in less particle loss during pipeline operation.

[0122] In this step, after the sample is drawn, it is transported to the detection chamber 33. The specific process is basically the same as the sampling step S2.1 in Example 1, which is described briefly below.

[0123] In method two, controller 1 controls the first fluid power source 31 to transport the sample, such that a portion of the sample is located to the left of the outlet of detection chamber 33 (within the first pipe g1 and / or the first fluid power source 31), a portion of the sample is located between the outlet and inlet of detection chamber 33 (i.e., detection chamber 33 is filled with sample), and the other portion of the sample is located to the right of detection chamber 33 (e.g., within the pipe between sampling needle a and the inlet of detection chamber 33). In other words, controller 1 controls the first fluid power source to transport the sample collected in this step, such that a portion of the sample flows through detection chamber 33, a portion of the sample fills detection chamber 33, and the other portion of the sample is located to the right of the inlet of detection chamber 33 (e.g., within the pipe between sampling needle a and the inlet of detection chamber 33). Similarly, before the sample enters detection chamber 33, controller 1 controls the moving device to drive the magnet to move to the back of detection chamber 33, generating a magnetic field. After the sample enters detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the action of the magnetic field.

[0124] Method 3: Controller 1 controls the first fluid power source 31 to transport the sample, placing it in the detection chamber 33; a portion of the cleaning fluid is then pumped out via the first fluid power source 31 to prevent air bubbles from forming in the detection chamber 33. In other words, controller 1 controls the first fluid power source 31 to transport the sample, placing it in the detection chamber 33; then, the first fluid power source 31 pumps out the cleaning fluid in reverse to prevent air bubbles from forming in the detection chamber 33. Similarly, before the sample enters the detection chamber 33, controller 1 controls the moving device to drive the magnet to the back of the detection chamber 33, generating a magnetic field. After the sample enters the detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the influence of the magnetic field.

[0125] Method 4: Controller 1 controls the first fluid power source 31 to transport the sample, positioning it in the detection chamber 33. Similarly, before the sample enters the detection chamber 33, controller 1 controls a moving device to drive a magnet to the back of the detection chamber 33, generating a magnetic field. After the sample enters the detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the influence of the magnetic field.

[0126] In detection step S3.2, controller 1 controls the detection device to detect the light signal of particle f in the detection chamber. Similarly, this step is the same as step S3.1 in the previous embodiment, so it will not be described again.

[0127] After cleaning step S4.2 and detection, controller 1 closes particle confinement device 4 or controls particle confinement device 4 away from detection chamber 33, so that particles in detection chamber 33 are not confined by particle confinement device 4, facilitating particle discharge. Then controller 1 controls drive device to position sampling needle a at waste liquid level. Of course, this action of controlling drive device to position sampling needle a at waste liquid level can also be performed before detection is completed, as long as it is performed after sampling, so that sampling needle is at waste liquid level during subsequent cleaning of sampling needle, and sampling needle a is connected to first fluid power source 31 through detection chamber 33. Then control first fluid power source 31 to provide power, and at least one section of cleaning liquid from first fluid power source 31 to first pipe g1, the sample to be tested in detection chamber 33, and the liquid from detection chamber 33 to sampling needle a are discharged through sampling needle a to waste liquid recovery device 38 to discharge waste liquid and clean detection chamber 33 and sampling needle a.

[0128] Example 3 ( Figure 8 The embodiment of the liquid circuit subsystem shown is equivalent to embodiment two. Figure 7Based on the above, the liquid circuit subsystem adds a first gate device 34; or, equivalently, based on Embodiment 1, the liquid circuit subsystem adds a second gate device 32. In this embodiment, the first gate device 34 has an outlet 1, a first inlet 2, and a second inlet 3. The first gate device 34 selects the detection chamber 33, the sampling needle a, and the second pipe g2. The outlet 1 of the first gate device 34 is connected to the inlet of the detection chamber 33, the first inlet 2 of the first gate device 34 is connected to the sampling needle a, and the second inlet 3 of the first gate device 34 is connected to the cleaning fluid container 37 for providing cleaning fluid through the second pipe g2. The first fluid power source 31 is connected to the outlet of the detection chamber 33 in sequence through the first pipe g1 and the second gate device 32.

[0129] The cleaning fluid container 37' connected to the third pipe g3 and the cleaning fluid container 37 connected to the second pipe g2 can be different cleaning fluid containers, for example, they are two independent containers. Of course, the cleaning fluid container 37' connected to the third pipe g3 and the cleaning fluid container 37 connected to the second pipe g2 can also be the same cleaning fluid container. For example, this cleaning fluid container is connected to the first gate device 34 through the second pipe g2 and to the second gate device 32 through the third pipe g3. It can be seen that the present invention does not limit the cleaning fluid container.

[0130] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0131] Preprocessing step S1.3: This embodiment is actually a combination of embodiment one and embodiment two. Therefore, this step can use the preprocessing step S1.2 of embodiment two for preprocessing.

[0132] In sampling step S2.3, controller 1 controls the second gate device 32 to switch the outlet of the detection chamber 33 to connect with the first fluid power source 31, and controls the first gate device 34 to switch the inlet of the detection chamber 33 to connect with the sampling needle a. Controller 1 controls the drive device to position the sampling needle a at the sampling position. Thus, the first fluid power source 31, the detection chamber 33, the sampling needle a, and the sample container 36 are connected. The first fluid power source 31 then provides power to allow the sampling needle a to draw a sample from the sample container 36 placed at the sampling position b and transport the drawn sample to the detection chamber 33. The amount of sample drawn depends on the detection requirements. In this invention, the sample enters the detection chamber 33 directly from the sample container 36, without the need for temporary storage in the sample loop of the prior art. Therefore, the process is not only simple and efficient, but also results in less particle loss during pipeline operation.

[0133] In this step, after the sample is drawn, it is transported to the detection chamber 33. The specific process is the same as that in the sampling step S2.1 of Example 1, and will not be described in detail here.

[0134] In detection step S3.3, controller 1 controls the detection device to detect the light signal of particle f in the detection chamber. Similarly, this step is the same as step S3.2 in the previous embodiment, so it will not be described again.

[0135] After cleaning step S4.3 and detection, controller 1 closes particle confinement device 4 or moves particle confinement device 4 away from detection chamber 33, so that particles in detection chamber 33 are not confined by particle confinement device 4, facilitating particle discharge. Then, controller 1 controls the drive device to position sampling needle a at the waste liquid discharge level, controls the first gate device 34 to switch, connecting the inlet of detection chamber 33 to sampling needle a, and controls the second gate device 32 to switch, connecting the outlet of detection chamber 33 to the first fluid power source 31. That is, sampling needle a is connected to the first fluid power source 31 through detection chamber 33, and the first fluid power source 31 provides power to discharge at least a section of cleaning liquid from the first fluid power source 31 to the first pipe g1, the sample to be tested in detection chamber 33, and the liquid from detection chamber 33 to sampling needle a through sampling needle a to the waste liquid recovery device 38 to discharge waste liquid and clean detection chamber 33 and sampling needle a.

[0136] Example 4 ( Figure 9 The embodiment of the liquid circuit subsystem shown is equivalent to... Figure 5 The illustrated liquid path includes an additional second gate device 32, a first gate device 34, and a second fluid power source 39. In this embodiment, the first fluid power source 31 is connected to the outlet of the detection chamber 33 via a first pipe g1 and the second gate device 32. The second gate device 32 has a drain port for discharging liquid towards the waste liquid recovery device 38'. There may be no pipe connection between the drain port and the waste liquid recovery device 38'; the waste liquid can be directly discharged from the drain port to the waste liquid recovery device 38'. Alternatively, a third pipe g3 can be connected to the drain port, discharging the waste liquid to the waste liquid recovery device 38' via the third pipe g3. This embodiment uses the latter as an example, where the drain port of the second gate device 32 is connected to the waste liquid recovery device 38' via the third pipe g3.

[0137] The second fluid power source 39 is used to provide power for the flow of liquid in the fluid circuit. The second fluid power source 39 is a device capable of driving the flow of liquid in the pipeline, such as various types of pumps or combinations of multiple pumps, or combinations of multiple pumps connected by pipes, valves, etc. The first fluid power source 31 and the second fluid power source 39 can be two independent fluid power devices, for example, each being a pump. Alternatively, the first fluid power source 31 and the second fluid power source 39 can be the same fluid power device, for example, both being the same pump, used for different purposes through different connections via valves, pipes, etc. Of course, the first fluid power source 31 and the second fluid power source 39 can also share certain pumps, which are used for different purposes. In the case where both are the same fluid power device, for example, the fluid power device has a port 1 for pumping in and out of liquid, and a port 2 for pumping in and out of liquid. Port 1 is connected to the second gate device 32, and port 2 is connected to the first gate device 34.

[0138] The second selection device 32 is used to select between the first fluid power source 31, the detection chamber 33, and the third pipe g3. Depending on the steps of particle detection, the selection method of the second selection device 32 in this embodiment can also be different. For example, the second selection device 32 can switch to connect the third pipe g3 to the outlet of the detection chamber 33, and then switch to connect the outlet of the detection chamber 33 to the first fluid power source 31. This selection method corresponds to the second fluid power source 39 providing power to clean the detection chamber 33 with cleaning fluid and then discharging the waste liquid after cleaning the detection chamber 33 into the waste liquid recovery device 38' connected to the third pipe g3. For example, the second gate device 32 switches to connect the third pipe g3 to the first fluid power source 31, and switches to connect the outlet of the detection chamber 33 to the first fluid power source 31. This gate method corresponds to the second fluid power source 39 providing power to clean the detection chamber 33 with cleaning fluid and discharge the waste liquid after cleaning the detection chamber 33 to the first fluid power source 31 and / or the second gate device 32 (including the first fluid power source 31, the first pipe g1 and / or the second gate device 32). Then the second gate device 32 switches, and the first fluid power source 31 provides power to discharge the waste liquid through the second gate device 32 into the waste liquid recovery device 38' connected to the third pipe g3.

[0139] The first gate device 34 in this embodiment has an outlet 1, a first inlet 2, a second inlet 3, and a third inlet 4. The first gate device 34 is used to select between the detection chamber 33, the sampling needle a, the second pipe g2, and the second fluid power source 39. Similarly, depending on the steps of particle detection, the gate selection method of the first gate device 34 in this embodiment can also be different. For example, the first gate device 34 can switch to connect outlet 1 with the second inlet 3, switch to connect outlet 1 with the first inlet 2, and switch to connect outlet 1 with the third inlet 4. This gate selection method corresponds to the first fluid power source 31 providing power to first draw cleaning fluid between the first gate device 34 and the first fluid power source 31, then the second fluid power source draws cleaning fluid between the first gate device 34 and the second fluid power source 39, and finally the second fluid power source 39 provides power to clean the detection chamber 33. As another example, the first gate device 34 can switch to connect the second inlet 3 with the third inlet 4, switch to connect outlet 1 with the first inlet 2, and switch to connect outlet 1 with the third inlet 4. This selection method corresponds to the second fluid power source 39 providing power to draw in cleaning fluid and use the cleaning fluid to clean the detection chamber 33.

[0140] The outlet 1 of the first gate device 34 is connected to the inlet of the detection chamber 33. For example, the outlet 1 of the first gate device 34 is directly connected to the inlet of the detection chamber 33 or connected through a pipe. The first inlet 2 of the first gate device is connected to the sampling needle a. The second inlet 3 of the first gate device 34 is connected to the cleaning fluid container 37 for providing cleaning fluid through the second pipe g2. The third inlet 4 of the first gate device 34 is connected to the second fluid power source 39. For example, the third inlet 4 of the first gate device 34 is directly connected to the second fluid power source 39 or connected through a pipe.

[0141] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0142] In the pretreatment step S1.4, the controller 1 controls the second gate device 32 to connect the first fluid power source 31 to the detection chamber 33. Then, the pretreatment step S1.1 of Embodiment 1 can be used for pretreatment. Alternatively, the controller 1 can control the first gate device 34 to connect the second fluid power source 39 to the cleaning fluid container 37. The second fluid power source 39 draws cleaning fluid from the cleaning fluid container 37. Then, the controller 1 controls the first gate device 34 to connect the detection chamber 33 to the second fluid power source 39. The second fluid power source 39 pushes the drawn cleaning fluid between the first fluid power source 31 and / or the second gate device.

[0143] In sampling step S2.4, controller 1 controls the second gate device 32 to connect the first fluid power source 31 to the detection chamber 33, controls the first gate device 34 to connect the inlet of the detection chamber 33 to the sampling needle a, and controls the drive device to position the sampling needle a at the sampling position. Then, controller 1 controls the first fluid power source 31 to provide power so that the sampling needle a draws the sample from the sample container 36 placed on the sampling position b. Controller 1 controls either the first fluid power source 31 or the second fluid power source 39 to transport the sample drawn into the liquid path to the detection chamber 33. In this embodiment, the first fluid power source 31 transports the drawn sample to the detection chamber 33. Therefore, this step is basically the same as sampling step S2.1 in Embodiment 1, and will not be described in detail here.

[0144] In detection step S3.4, controller 1 controls the detection device to detect the light signal of particle f in the detection chamber. Similarly, this step is the same as step S3.1 in Embodiment 1, so it will not be described again.

[0145] After cleaning step S4.4 and detection, controller 1 closes particle confinement device 4 or controls particle confinement device 4 away from detection chamber 33, so that particles in detection chamber 33 are not confined by particle confinement device 4, facilitating particle discharge, and then cleaning can proceed. Compared with the above embodiment, this embodiment adds a second fluid power source 39. The main difference between this embodiment and the above embodiment is that the second fluid power source 39 provides power to clean detection chamber 33. When cleaning sampling needle a and cleaning detection chamber 33, the flow direction of liquid in detection chamber is opposite. The cleaning liquid after cleaning detection chamber 33 is discharged to waste liquid recovery device 38' connected to the third pipe g3, and the cleaning liquid after cleaning sampling needle a is discharged to waste liquid recovery device 38 at the waste liquid discharge level.

[0146] For example, controller 1 controls the first gate device 34 to switch, connecting the inlet of detection chamber 33 to the second fluid power source 39, and controls the second gate device 32 to switch, connecting the outlet of detection chamber 33 to the third pipe g3. The second fluid power source 39 provides power to push the cleaning fluid into detection chamber 33 to clean it, and the fluid is discharged through the second gate device 32 and the third pipe g3 to the waste liquid recovery device 38'. Controller 1 controls the drive device to position the sampling needle a at the waste liquid discharge position, controls the first gate device 34 to switch, connecting the inlet of detection chamber 33 to the sampling needle a, and controls the second gate device 32 to switch, connecting the outlet of detection chamber 33 to the first fluid power source 31. The first fluid power source 31 provides power to discharge at least a section of cleaning fluid from the first fluid power source 31 to the sampling needle a, and the liquid in the sampling needle a through the sampling needle a to the waste liquid recovery device 38' to discharge waste liquid and clean the sampling needle a.

[0147] The waste liquid recovery device 38' connected to the third pipe g3 and the waste liquid recovery device 38 at the waste discharge level can be different waste liquid recovery devices, for example, they are two independent containers. Of course, the waste liquid recovery device 38' connected to the third pipe g3 and the waste liquid recovery device 38 at the waste discharge level can also be the same waste liquid recovery device. For example, the recovery device is connected to the first gate device 34 through the sampling needle a and to the second gate device 32 through the third pipe g3. It can be seen that the present invention does not limit the waste liquid recovery device.

[0148] Example 5 ( Figure 10 The embodiment of the liquid circuit subsystem shown is equivalent to... Figure 5 A new suction position d is added to the liquid path subsystem shown. The suction position d refers to the location where the sampling needle draws in the cleaning fluid. In one embodiment, this position can be a fixed facility, such as a recessed groove, for placing the cleaning fluid container 37 to provide the cleaning fluid. Alternatively, the suction position d can also be a container itself, used to hold the cleaning fluid. In some embodiments, the suction position d can also be a coordinate location; when cleaning fluid needs to be drawn, the sampling needle and / or the cleaning fluid container are moved to this location to collect the cleaning fluid.

[0149] Correspondingly, the driving device is also used to drive the sampling needle a and / or the aspiration position d to move, so that the sampling needle a is located at the aspiration position d when aspirating the cleaning fluid, facilitating the aspiration of the cleaning fluid from the cleaning fluid container 37. Specifically, the sampling needle a being located at the aspiration position means being positioned in a location adapted to the cleaning fluid container 37, at which position the sampling needle a can aspirate the cleaning fluid from the cleaning fluid container 37. The driving device also has three methods to position the sampling needle a at the aspiration position d when aspirating the cleaning fluid. The first method: the aspiration position d does not move, and the driving device only drives the sampling needle a to move, so that the sampling needle a is located at the aspiration position d when aspirating the cleaning fluid. The second method: the sampling needle a does not move, and the driving device only drives the aspiration position d to move, so that the sampling needle a is located at the aspiration position d when aspirating the cleaning fluid. The third of the three methods: The driving device drives the sampling needle a and the liquid aspiration position d to move, so that the sampling needle a is located at the liquid aspiration position d when aspirating the cleaning liquid; for example, the driving device drives the liquid aspiration position to move below the sampling needle a, drives the sampling needle a to move downward, so that the sampling end of the sampling needle a extends into the cleaning liquid container 37 on the liquid aspiration position d, so that the inlet of the detection chamber 33 is connected to the cleaning liquid container 37, and then the first fluid power source 31 generates power to draw the cleaning liquid into the liquid path.

[0150] The suction position d and the waste liquid discharge position c can be different workstations. For example, the drive subsystem 5 also includes a tray, on which one position is used to place the cleaning fluid container 37 (suction position d), and another position is used to place the waste liquid recovery device 38 (waste liquid discharge position c). Alternatively, the suction position d and the waste liquid discharge position c can be the same workstation. For example, the drive subsystem 5 also includes a tray, on which one position is used to place both the cleaning fluid container 37 and the waste liquid recovery device 38. When the cleaning fluid container 37 is placed at this position, it is the suction position d; when the waste liquid recovery device 38 is placed at this position, it is the waste liquid discharge position c. In practice, after absorbing the cleaning fluid, the cleaning fluid container 37 can also be used as the waste liquid recovery device 38 to receive waste liquid.

[0151] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0152] In the preprocessing step S1.5, the controller 1 controls the drive device to position the sampling needle a at the aspiration position d, and controls the first fluid power source 31 to provide power so that the sampling needle a can draw cleaning fluid at the aspiration position d. In this embodiment, the cleaning fluid container 37 is placed at the aspiration position d as an example. That is, the first fluid power source 31 is controlled to provide power so that the sampling needle a can draw cleaning fluid from the cleaning fluid container 37 at the aspiration position d until the entire liquid path is filled with cleaning fluid. Filling the entire liquid path with cleaning fluid before sampling can prevent air bubbles from appearing in the liquid path and avoid air bubbles interfering with the imaging of the detection chamber 33. Of course, filling the entire liquid path with cleaning fluid before sampling is not necessary. Regardless of whether the entire liquid path is filled with cleaning fluid, the controller 1 controls the drive device to position the sampling needle a at the aspiration position d, and controls the first fluid power source 31 to provide power so that the sampling needle a can draw cleaning fluid from the cleaning fluid container 37 at the aspiration position d. The cleaning fluid drawn this time is used for subsequent cleaning, and the amount of cleaning fluid drawn is sufficient for cleaning after particle detection.

[0153] In the sampling step S2.5, the controller 1 controls the drive device to position the sampling needle a in the sampling position, and controls the first fluid power source 31 to provide power so that the sampling needle a can draw the sample from the sample container 36 placed on the sampling position b and transport the drawn sample to the detection chamber 33.

[0154] In this step, after the sample is drawn, there are four ways to transport the drawn sample to the testing chamber 33. These are described below.

[0155] In method one, controller 1 controls the drive device to position the sampling needle a at the liquid aspiration position d, and controls the first fluid power source 31 to provide power to aspirate the cleaning liquid. Thus, a liquid flow is formed in the liquid path with the cleaning liquid aspirated in step S1.5 first, the sample aspirated in this step in the middle, and the cleaning liquid aspirated in this step last. Controller 1 controls the first fluid power source 31 to transport this liquid flow. First, the cleaning liquid in the liquid flow passes through the detection chamber 33. Before the sample enters the detection chamber 33, controller 1 controls the moving device to drive the magnet to move to the back of the detection chamber 33, generating a magnetic field. The first fluid power source 31 continues to aspirate the liquid. The sample enters the detection chamber 33, and the particles f in the sample are arranged in a single layer and fixed under the action of the magnetic field. At this time, the first fluid power source 31 can stop working. Preferably, the first fluid power source 31 continues to aspirate the liquid before stopping, so that part of the cleaning liquid after the sample flows through the detection chamber 33, completing the rinsing of the particles f (the sample contains other impurities besides particles f; the cleaning liquid flowing through the detection chamber 33 carries away the impurities, while the particles f are fixed by the magnetic force and will not be carried away).

[0156] In method two, controller 1 controls the first fluid power source 31 to transport the aspirated sample, positioning a portion of the sample to the left of the outlet of detection chamber 33 (within the first pipe g1 and / or the first fluid power source 31), a portion between the outlet and inlet of detection chamber 33 (i.e., detection chamber 33 is filled with sample), and the remaining portion to the right of the outlet of detection chamber 33. Similarly, before the sample enters detection chamber 33, controller 1 controls a moving device to drive a magnet to the back of detection chamber 33, generating a magnetic field. After the sample enters detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the influence of the magnetic field.

[0157] Method 3 is the same as Method 3 in Example 1, so it will not be described in detail.

[0158] Method four is the same as Method four in Example 1, so it will not be described in detail.

[0159] The detection step S3.5 is the same as the detection step in Example 1, so it will not be described again.

[0160] The cleaning steps S4.5 are the same as those in Example 2, so they will not be described in detail.

[0161] Example 6 ( Figure 11The embodiment of the liquid circuit subsystem shown is equivalent to adding a second gate device 32 to the previous embodiment; it is also equivalent to adding a suction position d to the second embodiment. The first fluid power source 31 is connected to the outlet of the detection chamber 33 through the first pipe g1 and the second gate device 32 in sequence. The second gate device 32 is also connected to a cleaning fluid container 37' for providing cleaning fluid through the third pipe g3. The cleaning fluid container 37' connected to the third pipe g3 and the cleaning fluid container 37 on the suction position d can be different cleaning fluid containers, for example, they are two independent containers. Of course, the cleaning fluid container 37' connected to the third pipe g3 and the cleaning fluid container 37 on the suction position d can also be the same cleaning fluid container. For example, the cleaning fluid container is placed on the suction position d and connected to the second gate device 32 through the third pipe g3; it can be seen that the present invention does not limit the cleaning fluid container.

[0162] The second selection device is used to select the first fluid power source 31, the third pipeline g3 and the detection chamber 33. Specifically, it connects at least one of the third pipeline g3 and the detection chamber 33 to the first fluid power source 31.

[0163] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0164] In pretreatment step S1.6, controller 1 controls the second gate device 32 to connect the first fluid power source 31 to the third pipe g3, and controls the first fluid power source 31 to provide power so that the third pipe g3 draws cleaning fluid from the cleaning fluid container 37' (same as pretreatment step S1.2 in embodiment two). Both methods can ensure that the first fluid power source 31 and / or the first pipe g1 contain cleaning fluid, which facilitates subsequent cleaning.

[0165] In sampling step S2.6, controller 1 controls the second gate device 32 to connect the first fluid power source 31 to the outlet of the detection chamber 33. Controller 1 controls the drive device to position the sampling needle a at the sampling position, and controls the first fluid power source 31 to provide power so that the sampling needle a can draw the sample from the sample container 36 placed at the sampling position b and transport the drawn sample to the detection chamber 33. This step is the same as sampling step S2.5 in the previous embodiment, and therefore will not be described again.

[0166] The detection step S3.6 is the same as the detection step in the previous embodiment, so it will not be described again.

[0167] The cleaning step S4.6 is the same as the cleaning step in the previous embodiment, so it will not be described again.

[0168] Example 7 ( Figure 12 The embodiment of the liquid circuit subsystem shown is equivalent to... Figure 9 The cleaning fluid container selected by the first selection device has been changed to the cleaning fluid container on the suction position, and the connection between the cleaning fluid container and the detection chamber 33 is controlled by the drive device. This is equivalent to... Figure 5 The liquid path shown is supplemented with a second selection device 32, a first selection device 34, a second fluid power source 39, and a liquid suction level d.

[0169] The second fluid power source 39 is used to provide power for the flow of liquid in the fluid circuit. The second fluid power source 39 is a device capable of driving the flow of liquid in the pipeline, such as various types of pumps or combinations of multiple pumps, or combinations of multiple pumps connected by pipes, valves, etc. The first fluid power source 31 and the second fluid power source 39 can be two independent fluid power devices, for example, each being a pump. Alternatively, the first fluid power source 31 and the second fluid power source 39 can be the same fluid power device, for example, both being the same pump, used for different purposes through different connections via valves, pipes, etc. Of course, the first fluid power source 31 and the second fluid power source 39 can also share certain pumps, which are used for different purposes. In the case where both are the same fluid power device, for example, the fluid power device has a port 1 for pumping in and out of liquid, and a port 2 for pumping in and out of liquid. Port 1 is connected to the second gate device 32, and port 2 is connected to the first gate device 34.

[0170] The second selection device 32 is used to select between the first fluid power source 31, the detection chamber 33, and the drain outlet. This embodiment takes the selection of the first fluid power source 31, the detection chamber 33, and the third pipe g3 as an example. Depending on the steps of particle detection, the selection method of the second selection device 32 in this embodiment can also be different. For example, the second selection device 32 can switch to connect the third pipe g3 to the outlet of the detection chamber 33, and switch to connect the outlet of the detection chamber 33 to the first fluid power source 31. This selection method corresponds to the second fluid power source 39 providing power to clean the detection chamber 33 with cleaning fluid and discharge the waste liquid after cleaning the detection chamber 33 into the waste liquid recovery device 38' connected to the third pipe g3. For example, the second gate device 32 switches to connect the third pipe g3 to the first fluid power source 31, and switches to connect the outlet of the detection chamber 33 to the first fluid power source 31. This gate method corresponds to the second fluid power source 39 providing power to clean the detection chamber 33 with cleaning fluid and discharge the waste liquid after cleaning the detection chamber 33 between the first fluid power source 31 and / or the second gate device. Then the second gate device 32 switches, and the first fluid power source 31 provides power to discharge the waste liquid through the second gate device 32 into the waste liquid recovery device 38' connected to the third pipe g3.

[0171] The first gate device 34 in this embodiment has an outlet 1, a first inlet 2, and a second inlet 3. The first gate device 34 is used to select between the detection chamber 33, the sampling needle a, and the second fluid power source 39. This can be done in several ways. For example, one method is to connect the outlet 1 of the first gate device to its first inlet 2, and the first gate device controls the connection and disconnection between its outlet 1 and its second inlet 3 (e.g., by installing a valve between the outlet 1 and the second inlet 3). Another method is to connect the outlet 1 of the first gate device to its second inlet 3, and the first gate device controls the connection and disconnection between its outlet 1 and its first inlet 2 (e.g., by installing a valve between the outlet 1 and the first inlet 2). Yet another method is to at least connect the outlet 1 to the first inlet 2 and then to the second inlet 3 via switching. In optional embodiments, it can also connect the first inlet 2 to the second inlet 3 via switching. This embodiment uses the last method of the first gate device as an example for explanation.

[0172] In this embodiment, the first fluid power source 31 is connected to the outlet of the detection chamber 33 via the first pipe g1 and the second gate device 32. The second gate device 32 is also connected to the waste liquid recovery device 38' via the third pipe g3. The outlet 1 of the first gate device 34 is connected to the inlet of the detection chamber 33. For example, the outlet 1 of the first gate device 34 is directly connected to the inlet of the detection chamber 33 or connected via a pipe. The first inlet 2 of the first gate device 34 is connected to the sampling needle, and the second inlet 3 of the first gate device 34 is connected to the second fluid power source 39. For example, the second inlet 3 of the first gate device 34 is directly connected to the second fluid power source 39 or connected via a pipe.

[0173] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0174] In pretreatment step S1.7, controller 1 controls the second gate device 32 to connect the first fluid power source 31 to the detection chamber 33, controller 1 controls the first gate device 34 to connect the inlet of the detection chamber 33 to the sampling needle a, controller 1 controls the drive device to position the sampling needle a at the liquid aspiration position d, and controls the first fluid power source 31 to provide power so that the sampling needle a draws cleaning fluid from the cleaning fluid container 37 until the entire liquid path is filled with cleaning fluid. It is not necessary to fill the entire liquid path with cleaning fluid before sampling. Regardless of whether the entire liquid path is filled with cleaning fluid, controller 1 controls the second gate device 32 to connect the first fluid power source 31 to the detection chamber 33, controls the first gate device 34 to connect the inlet of the detection chamber 33 to the sampling needle a, controller 1 controls the drive device to position the sampling needle a at the liquid aspiration position d, and controls the first fluid power source 31 to provide power so that the sampling needle a draws cleaning fluid from the cleaning fluid container 37. The cleaning fluid drawn this time is used for subsequent cleaning.

[0175] Of course, the controller 1 can also control the first gate device 34 to connect the second fluid power source 39 to the sampling needle a, control the drive device to make the sampling needle a in the liquid suction position, the second fluid power source 39 draws cleaning liquid from the cleaning liquid container 37, and then control the first gate device 34 to connect the detection chamber 33 to the second fluid power source 39, and the second fluid power source 39 pushes the drawn cleaning liquid into the first fluid power source 31 and / or the first pipe g1 for subsequent cleaning.

[0176] In sampling step S2.7, controller 1 controls the second gate device 32 to connect the first fluid power source 31 to the detection chamber 33, controls the first gate device 34 to connect the inlet of the detection chamber 33 to the sampling needle a, and controls the drive device to position the sampling needle a at the sampling position. Then, controller 1 controls the first fluid power source 31 to provide power so that the sampling needle a draws the sample from the sample container 36 placed on the sampling position b. Controller 1 controls either the first fluid power source 31 or the second fluid power source 39 to transport the sample drawn into the liquid path to the detection chamber 33. In this embodiment, the first fluid power source 31 transports the drawn sample to the detection chamber 33.

[0177] In this step, after the sample is drawn, there are four ways to transport the drawn sample to the testing chamber 33. These are described below.

[0178] In method one, controller 1 controls the first gate device 34 to connect the inlet of the detection chamber 33 with the sampling needle a, controls the drive device to position the sampling needle a at the liquid aspiration position d, and controls the first fluid power source 31 to aspirate the cleaning fluid. Controller 1 controls the first fluid power source 31 to transport the sample and cleaning fluid. First, the cleaning fluid passes through the detection chamber 33. Before the sample enters the detection chamber 33, controller 1 controls the moving device to drive the magnet to move to the back of the detection chamber 33, generating a magnetic field. The first fluid power source 31 continues to aspirate the liquid. The sample enters the detection chamber 33, and the particles f in the sample are arranged in a single layer and fixed under the action of the magnetic field. At this time, the first fluid power source 31 can stop working. Preferably, the first fluid power source 31 continues to aspirate the liquid before stopping, so that some of the cleaning fluid after the sample flows through the detection chamber 33, completing the rinsing of the particles f.

[0179] Method 2 is the same as Method 2 in Implementation Example 1, so it will not be described in detail.

[0180] Method 3 is the same as Method 3 in Implementation Example 1, so it will not be described in detail.

[0181] Method four is the same as Method four in Implementation Example 1, so it will not be described in detail.

[0182] The detection step S3.7 is the same as the detection step in Example 1, so it will not be described again.

[0183] The cleaning step S4.7 is the same as the cleaning step in Example 4, so it will not be described again.

[0184] The waste liquid recovery device 38' connected to the third pipe g3 and the waste liquid recovery device 38 at the waste discharge level can be different waste liquid recovery devices, for example, they are two independent containers. Of course, the waste liquid recovery device 38' connected to the third pipe g3 and the waste liquid recovery device 38 at the waste discharge level can also be the same waste liquid recovery device. For example, the recovery device is connected to the first gate device 34 through the sampling needle a and to the second gate device 32 through the third pipe g3. It can be seen that the present invention does not limit the waste liquid recovery device.

[0185] In the above embodiments, both sample aspiration and needle washing are powered by the first fluid power source 31. In subsequent embodiments, sample aspiration is powered by the first fluid power source 31, and needle washing is powered by the second fluid power source 39. A detailed schematic diagram is shown below. Figure 17 As shown, the fluid circuit subsystem includes at least: a sampling needle a, a detection chamber 33, a first fluid power source 31, a first gating device 34 for selective connection, and a second fluid power source 39. The first fluid power source 31 is connected to the outlet of the detection chamber 33. The inlet of the detection chamber 33 and the second fluid power source 39 are both connected to the sampling needle a through the first gating device 34.

[0186] The sampling needle a is used to draw up samples with the power provided by the first fluid power source 31 and to discharge cleaning fluid with the power provided by the second fluid power source 39 to clean the sampling needle.

[0187] like Figure 17 As shown, the detection system also includes: a sampling position b, a waste liquid discharge position c, and a drive device.

[0188] A driving device for driving sampling needle a and / or aspiration position b to move so that sampling needle a is located at aspiration position b when aspiration is performed, and for driving sampling needle a and / or waste liquid position c to move so that sampling needle a is located at waste liquid position c when waste liquid is discharged.

[0189] Controller 1 controls the optical path subsystem 2, the liquid path subsystem 3, the particle confinement device 4, and the drive subsystem 5 to detect one or more particles. For example, controller 1 controls the first gating device 34 to select, the first fluid power source 31 to operate, the second fluid power source 39 to operate, and the drive device to drive. When controller 1 controls the drive device to position the sampling needle a at the sampling position b, it controls the first gating device 31 to connect the sampling needle a to the inlet of the detection chamber 33, controls the first fluid power source 31 to provide power so that the sampling needle a can pick up the sample at the sampling position b, and controls the first fluid power source 31 or the second fluid power source 39 to drive. The sample drawn into the liquid path is transported to the detection chamber 33. For example, the first fluid power source 31 provides power to make the sampling needle a draw up the sample at the sampling position b and transport the drawn up sample to the detection chamber 33. Alternatively, the first fluid power source 31 is controlled to provide power to make the sampling needle a draw up the sample at the sampling position b. After the sampling is completed, the first gate device 34 is controlled to connect the second fluid power source 39 to the inlet of the detection chamber 33, and the second fluid power source 39 is controlled to transport the sample drawn into the liquid path to the detection chamber 33 (the first fluid power source 31 can also be controlled simultaneously to assist in transporting the sample drawn into the liquid path to the detection chamber 33). The controller 1 controls the drive device to position the sampling needle a at the sampling position, and the controller 1 controls the first gate device 34 to connect the inlet of the detection chamber 33 to the sampling needle a. These two actions can be performed simultaneously without a specific order. After sampling is completed, controller 1 controls the first gate device 34 to connect the sampling needle a and the second fluid power source 39. Controller 1 controls the second fluid power source 39 to provide power. Before the cleaning fluid is discharged through the sampling needle a to clean it, controller 1 also controls the drive device to position the sampling needle a at the waste liquid level c, facilitating the discharge of the cleaning fluid from cleaning the sampling needle a to the waste liquid recovery device at the waste liquid level c. This action of controlling the drive device to position the sampling needle a at the waste liquid level c can be performed after sampling to ensure that the sampling needle a is at the waste liquid level c during cleaning; for example, controller 1 can immediately control the drive device to position the sampling needle a at the waste liquid level c after sampling, or controller 1 can control the drive device to position the sampling needle a at the waste liquid level c after sampling is completed. After sampling, this embodiment will be described using the completion of sampling as an example. Controller 1 controls the drive device to position the sampling needle a at the waste liquid level c. After sampling is completed, the first gate device 34 is controlled to connect the sampling needle a and the second fluid power source 39. The drive device positions the sampling needle a at the waste liquid level c, and the first gate device 34 connects the sampling needle a and the second fluid power source 39. The order of these actions is not limited. Then, the second fluid power source 39 is controlled to provide power to discharge the cleaning solution through the sampling needle a to clean it. The sampling is considered complete when the sample in the sample container 36 is drawn into the liquid path, and the cleaning solution discharged from the second fluid power source 39 to clean the sampling needle a does not affect the final sample detection.For example, sample aspiration is considered complete once the sample in sample container 36 is drawn into the liquid path and a sufficient amount of sample required for detection exits from outlet 1 of the first gate device 34. Afterwards, the second fluid power source 39 discharges cleaning fluid to clean the sampling needle a without affecting the final sample detection. Of course, sample aspiration is also considered complete even if the required amount of sample does not exit from outlet 1 of the first gate device 34, as long as the second fluid power source 39's discharge of cleaning fluid to clean the sampling needle a does not affect the final sample detection (i.e., after sample aspiration, the subsequent liquid path will always drive a sufficient amount of sample to exit from outlet 1 of the first gate device 34). The working time of the fluid power source is a control parameter; therefore, sample aspiration can also be considered complete when a preset aspiration time is reached. The preset time is set according to requirements to ensure the required amount of sample for detection and that the second fluid power source 39's discharge of cleaning fluid to clean the sampling needle a does not affect the final sample detection.

[0190] Furthermore, the second fluid power source 39 provides power to discharge the cleaning solution through the sampling needle a to clean the sampling needle a. Specifically, the cleaning solution in the second fluid power source 39 and / or the cleaning solution between the second fluid power source 39 and the first gate device 34 is discharged through the sampling needle a to clean the sampling needle a. The cleaning solution in the second fluid power source 39 and / or the cleaning solution between the second fluid power source 39 and the first gate device 34 can be pre-absorbed by the first fluid power source 31 or pre-absorbed by the second fluid power source 39. Since the same sampling needle a is used for both sample aspiration and cleaning solution discharge, the process of discharging the cleaning solution is the process of cleaning the sampling needle a, making the cleaning process simple and efficient. Because the second fluid power source 39 is used to provide power for cleaning the sampling needle a, the cleaning of the sampling needle a and the sample detection in the detection chamber 33 can be performed simultaneously, resulting in high efficiency.

[0191] Furthermore, Figure 17 In the schematic diagram shown, the first fluid power source 31 is connected to the outlet of the detection chamber 33. It can be directly connected to the outlet of the detection chamber 33, or it can be connected to the outlet of the detection chamber 33 through the first pipe g1.

[0192] based on Figure 17 As shown in the schematic diagram above, sample loading and waste liquid discharge are performed automatically. Of course, users can also operate them manually. For example, without the need for a sample suction position, waste liquid discharge position, and drive device, the user can manually place the sample container 36 under the sampling needle a and manually place the waste liquid recovery device under the sampling needle a during sample loading.

[0193] In the manual mode, controller 1 controls the optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles. For example, the user manually places the sample container 36 below the sampling needle a, ensuring the needle a is below the liquid surface. Controller 1 then controls the first fluid power source 31 to provide power so that the sampling needle a draws the sample from the sample container 36. It then controls either the first fluid power source 31 or the second fluid power source 39 to transport the drawn sample to the detection chamber 33. After sampling, the user manually places the waste liquid recovery device below the sampling needle a. The controller controls the first gate device to connect the sampling needle to the second fluid power source, and controls the second fluid power source to provide power to discharge the pre-drawn cleaning solution through the sampling needle to the waste liquid recovery device to clean the needle. As can be seen, the manual mode primarily involves the user manually replacing the drive device and does not require a sampling position or a waste liquid discharge position. Other processes are the same as in the automated mode and will not be described in detail.

[0194] Figure 17 This is a schematic diagram of a liquid circuit subsystem. Specific liquid circuit subsystems can take many forms, such as... Figure 9 , 12 As shown in 18 and 19. The following will respectively... Figure 9 , 12 The four liquid circuit subsystems shown in 18 and 19 are described one by one as four embodiments.

[0195] Example 8, as Figure 9 As shown, the liquid circuit connection relationship in this embodiment is the same as that in embodiment four. The difference lies in the function of the first selection device 34 and the process of the controller 1 controlling the liquid circuit subsystem.

[0196] In this embodiment, the first gate device 34 is used to select between outlet 1, first inlet 2, second inlet 3, and third inlet 4 in pairs, so as to realize that the first fluid power source 31 provides power to aspirate the sample, the first fluid power source 31 and / or the second fluid power source 39 provides power to aspirate the cleaning fluid, and the second fluid power source 39 provides power to discharge the cleaning fluid to clean the sampling needle a. Specifically, there are three gate modes: one is that the first gate device 34 can select at least between outlet 1 and first inlet 2, first inlet 2 and third inlet 4, outlet 1 and second inlet 3, and second inlet 3 and third inlet 4. Another is that the first gate device 34 can select at least between outlet 1 and first inlet 2, first inlet 2 and third inlet 4, outlet 1 and second inlet 3, and outlet 1 and third inlet 4. Yet another is that the first gate device 34 can select at least between outlet 1 and first inlet 2, first inlet 2 and third inlet 4, second inlet 3 and third inlet 4, and outlet 1 and third inlet 4.

[0197] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0198] Preprocessing step S1.8: This step is the same as the preprocessing step in Example 4, so it will not be described again.

[0199] In sampling step S2.8, controller 1 controls the second gate device 32 to connect the first fluid power source 31 to the detection chamber 33, controls the first gate device 34 to connect the inlet of the detection chamber 33 to the sampling needle a, and controls the drive device to position the sampling needle a at the sampling position. Then, the first fluid power source 31 provides power to allow the sampling needle a to draw the sample from the sample container 36 placed at the sampling position b. In this embodiment, the first fluid power source 31 transports the drawn sample to the detection chamber 33. The specific process is the same as the sampling step in Embodiment 1, and therefore will not be described in detail.

[0200] In detection step S3.8, controller 1 controls the detection device to detect the optical signal of particle f in the detection chamber. Similarly, this step is the same as the detection step in Example 1, and therefore will not be described again.

[0201] After cleaning step S4.8 and detection, controller 1 closes particle confinement device 4 or moves particle confinement device 4 away from detection chamber 33, so that particles in detection chamber 33 are not confined by particle confinement device 4, facilitating particle discharge. Then, detection chamber 33 can be cleaned. Cleaning of detection chamber 33 can be powered by the first fluid power source 31 or the second fluid power source 39. Cleaning of sampling needle a can be performed during the sampling step, the detection step, or the cleaning step, as long as it is performed after sampling is completed. This embodiment does not impose any limitations. For example, before the test is completed, the controller 1 controls the first gate device 34 to connect the second fluid power source 39 to the cleaning fluid container 37, and controls the second fluid power source 39 to draw cleaning fluid from the cleaning fluid container 37; after the test is completed, the controller 1 controls the first gate device 34 to connect the second fluid power source 39 to the sampling needle a, controls the drive device to place the sampling needle a at the waste liquid level c, and controls the second fluid power source 39 to discharge a portion of the previously drawn cleaning fluid to the waste liquid recovery device 38 at the waste liquid level c to clean the sampling needle a; the controller 1 controls the first gate device 34 and the second gate device 32 to connect the second fluid power source 39, the detection chamber 33 and the waste liquid recovery device 38', and controls the second fluid power source 39 to provide power to discharge another portion of the cleaning fluid through the first gate device 34, the detection chamber 33 and the second gate device 32 in sequence into the waste liquid recovery device 38', thereby cleaning the detection chamber 33.

[0202] Example 9, as Figure 12As shown, the liquid circuit connection relationship in this embodiment is the same as that in embodiment seven, except that the controller 1 controls the liquid circuit subsystem.

[0203] The first gate device 34 is used for gate selection, and it can have multiple gate selection methods. For example, one method is that the outlet 1 of the first gate device is connected to its first inlet 2, that is, the sampling needle and the inlet of the detection chamber can be connected through the first gate device 34. The first gate device is used to control the connection and disconnection between its first inlet 2 and its second inlet 3 (e.g., by setting a valve between the first inlet 2 and the second inlet 3). Another method is that the first inlet 2 of the first gate device is connected to its second inlet 3, that is, the sampling needle and the second fluid power source can be connected through the first gate device. The first gate device is used to control the connection and disconnection between its outlet 1 and its first inlet 2 (e.g., by setting a valve between the outlet 1 and the first inlet 2). Yet another method is that the first gate device 34 is used to select between outlet 1, first inlet 2 and second inlet 3 in pairs, so as to realize that the first fluid power source 31 provides power to draw samples, the first fluid power source 31 or the second fluid power source 39 provides power to draw cleaning fluid, and the second fluid power source 39 provides power to discharge cleaning fluid to clean the sampling needle a. This embodiment uses the last method of the first gate device 34 as an example for explanation. The first gating device 34 is specifically used to connect the first inlet 2 to the second inlet 3 through switching, and to connect the outlet 1 to the first inlet 2 through switching. In an optional embodiment, it is also used to connect the outlet 1 to the second inlet 3 through switching.

[0204] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0205] Preprocessing step S1.9: This step is the same as the preprocessing step in Example 7, so it will not be described again.

[0206] In sampling step S2.9, controller 1 controls the second gate device 32 to connect the first fluid power source 31 to the detection chamber 33, controls the first gate device 34 to connect the inlet of the detection chamber 33 to the sampling needle a, and controls the drive device to position the sampling needle a at the sampling position. Then, the first fluid power source 31 provides power to allow the sampling needle a to draw the sample from the sample container 36 placed at the sampling position b. In this embodiment, the first fluid power source 31 transports the drawn sample to the detection chamber 33. The specific process is the same as the sampling step in embodiment seven, and therefore will not be described in detail.

[0207] In detection step S3.9, controller 1 controls the detection device to detect the optical signal of particle f in the detection chamber. Similarly, this step is the same as the detection step in Example 1, and therefore will not be described again.

[0208] After cleaning step S4.9 and detection, controller 1 closes particle confinement device 4 or controls particle confinement device 4 to move away from detection chamber 33, so that particles in detection chamber 33 are not confined by particle confinement device 4, facilitating particle discharge. Then, detection chamber 33 can be cleaned. The cleaning of detection chamber 33 can be powered by the first fluid power source 31 or the second fluid power source 39. Similar to the previous embodiment, for cleaning sampling needle a, controller 1 controls the drive device to position sampling needle a at waste liquid level c, controls the first gate device 34 to connect sampling needle a and the second fluid power source 39, and controls the second fluid power source 39 to provide power to discharge cleaning liquid through sampling needle a to waste liquid recovery device 38 to clean sampling needle a. This process can be performed in the sampling step, the detection step, or the cleaning step, as long as it is performed after sampling is completed; this embodiment does not limit this.

[0209] Based on embodiment nine, the present invention also provides another embodiment, such as... Figure 20 As shown, Figure 20 The liquid path shown is Figure 12 The difference in the fluid circuits shown is: Figure 12 In the liquid path shown, the detection chamber 33, the second fluid power source 39, and the sampling needle a are connected by a first gating device; and Figure 20 The detection chamber 33, the second fluid power source 39, and the sampling needle a in the liquid circuit shown are connected by a three-way connector 341. The three-way connector 341 has a first interface 1, a second interface 2, and a third interface 3. The first interface 1, the second interface 2, and the third interface 3 of the three-way connector 341 are interconnected. The first interface 1 of the three-way connector 341 is connected to the inlet of the detection chamber 33, the second interface 2 is connected to the sampling needle a, and the third interface 3 is connected to the second fluid power source 39. Other connections in the liquid circuit are the same. Figure 12 The fluid circuit will not be elaborated upon.

[0210] The controller is used to control the first fluid power source to provide power so that the sampling needle can draw up the sample, and to control the first fluid power source or the second fluid power source to transport the sample drawn into the liquid path to the detection chamber; the controller is also used to control the second fluid power source to provide power so that the cleaning fluid can be discharged through the sampling needle to clean the sampling needle.

[0211] Similarly, for sample loading, this embodiment can use either a manual or automatic method. The manual method involves manual assistance with sample loading, similar to the embodiments described above, and therefore will not be repeated. The automatic method also includes the driving device described above.

[0212] The controller controls the operation of the first fluid power source, the second fluid power source, and the drive device. When the controller controls the drive device to position the sampling needle at the aspiration position, it controls the first fluid power source to provide power so that the sampling needle can aspirate the sample at the aspiration position. The controller then controls either the first or second fluid power source to transport the sample aspirated into the liquid path to the detection chamber. The controller also controls the drive device to position the sampling needle at the waste liquid level and controls the second fluid power source to provide power to discharge cleaning fluid through the sampling needle to clean it. For example, after aspiration, the controller controls the drive device to position the sampling needle at the waste liquid level. After aspiration is complete, it controls the second fluid power source to provide power so that the sampling needle can discharge cleaning fluid at the waste liquid level to clean it. Taking time control as an example, after aspiration, the controller controls the drive device to position the sampling needle at the waste liquid level. After a preset aspiration time, it controls the second fluid power source to provide power so that the sampling needle can discharge cleaning fluid at the waste liquid level to clean it. Regardless of whether it is manual or automatic, the detection process in this embodiment is basically the same as in the above embodiments, and therefore will not be described in detail.

[0213] Example 10, as follows Figure 18 As shown, the fluid circuit connection relationship in this embodiment is... Figure 17 Based on this, the first gate device 34 also has a drain port for draining liquid. Furthermore, this embodiment also includes a cleaning liquid container, which can be connected to the liquid path through the second gate device disposed between the first fluid power source 31 and the detection chamber 33 to provide cleaning liquid to the liquid path; the cleaning liquid container can also be connected to the liquid path through the first gate device 34 to provide cleaning liquid to the liquid path. This embodiment will be described using the latter as an example.

[0214] like Figure 18 As shown, the first gating device 34 in this embodiment is used to selectively connect the detection chamber 33, sampling needle a, second fluid power source 39, second waste liquid recovery device 38', and cleaning liquid container 37. The first gating device 34 is used to connect the inlet of the detection chamber 33 to the sampling needle a; to connect the second fluid power source 39 to the sampling needle a; and to connect the inlet of the detection chamber 33 to the cleaning liquid container 37. The first gating device 34 is also used to connect the inlet of the detection chamber 33 to the second waste liquid recovery device 38', or to connect the second fluid power source 39 to the second waste liquid recovery device 38'. This enables the first fluid power source 31 to clean the detection chamber 33, and the second fluid power source 39 to clean the sampling needle a. Specifically, as shown... Figure 18As shown, the first gate device 34 includes a valve with five ports 1-5. Port 1 is connected to the inlet of the detection chamber 33, port 2 is connected to the sampling needle a, port 3 is connected to the second fluid power source 39, port 4 can serve as a drain port for discharging liquid into the second waste liquid recovery device 38', and port 5 is connected to the cleaning liquid container 37. The ports of this valve can be selectively connected in pairs under the control of the controller 1 to achieve the following: the first fluid power source 31 provides power to draw samples; the first fluid power source 31 and / or the second fluid power source 39 provides power to draw cleaning liquid; the first fluid power source 31 provides power to clean the detection chamber 33; and the second fluid power source 39 provides power to discharge cleaning liquid to clean the sampling needle a.

[0215] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0216] Preprocessing step S1.10: This step is the same as the preprocessing step in Example 7, so it will not be described again.

[0217] In sampling step S2.10, controller 1 controls the first gate device 34 to connect the inlet of the detection chamber 33 to the sampling needle a, and controls the drive device to position the sampling needle a at the sampling position. Then, the first fluid power source 31 provides power to allow the sampling needle a to draw the sample from the sample container 36 placed on the sampling position b. In this embodiment, the first fluid power source 31 transports the drawn sample to the detection chamber 33. The specific process is the same as the sampling step in embodiment seven, and therefore will not be described in detail.

[0218] In detection step S3.10, controller 1 controls the detection device to detect the light signal of particle f in the detection chamber. Similarly, this step is the same as the detection step in Embodiment 1, so it will not be described again.

[0219] After cleaning step S4.10 and detection, controller 1 closes particle confinement device 4 or controls particle confinement device 4 away from detection chamber 33, so that particles in detection chamber 33 are not confined by particle confinement device 4, facilitating particle discharge. Then, detection chamber 33 can be cleaned. The cleaning of detection chamber 33 is powered by the first fluid power source 31. Similar to the previous embodiment, for cleaning sampling needle a, controller 1 controls the drive device to position sampling needle a at waste liquid level c, controls the first gate device 34 to connect sampling needle a and the second fluid power source 39, and controls the second fluid power source 39 to provide power to discharge cleaning liquid through sampling needle a to the first waste liquid recovery device 38 to clean sampling needle a. This process can be carried out in the sampling step, the detection step, or the cleaning step. The aspiration and discharge of cleaning liquid can also be carried out in different steps, as long as they are carried out after the sampling is completed. This embodiment does not limit this.

[0220] Example 11, as follows Figure 19 As shown, the fluid circuit connection relationship in this embodiment is... Figure 17 Based on this, the first gate device 34 also has a drain port for draining liquid, and the detection system also has a suction position d. In this embodiment, the first gate device 34 is used to selectively connect the detection chamber 33, the sampling needle a, the second fluid power source 39, and the second waste liquid recovery device 38'. There can be multiple gate configurations. For example, one configuration is that the outlet 1 of the first gate device is connected to its first inlet 2, meaning that the sampling needle and the inlet of the detection chamber can be connected through the first gate device 34. The first gate device is used to control the connection and disconnection between its first inlet 2 and its second inlet 3 (e.g., by setting a valve between the first inlet 2 and the second inlet 3). Another configuration is that the first inlet 2 of the first gate device is connected to its second inlet 3, meaning that the sampling needle and the second fluid power source can be connected through the first gate device. The first gate device is used to control the connection and disconnection between its outlet 1 and its first inlet 2 (e.g., by setting a valve between the outlet 1 and the first inlet 2). Another method (this embodiment uses this method as an example) is that the first gate device 34 is used to switch the inlet of the detection chamber 33 to the sampling needle a; and to switch the second fluid power source 39 to the sampling needle a. The first gate device 34 is also used to switch the inlet of the detection chamber 33 to the second waste liquid recovery device 38', or to switch the second fluid power source 39 to the second waste liquid recovery device 38'. This allows the first fluid power source 31 to clean the detection chamber 33, and the second fluid power source 39 to clean the sampling needle a. Specifically, as shown... Figure 19 As shown, the first gate device 34 includes a valve with four ports 1-4. Port 1 is connected to the inlet of the detection chamber 33, port 2 is connected to the sampling needle a, port 3 is connected to the second fluid power source 39, and port 4 can serve as a drain port for discharging liquid into the second waste liquid recovery device 38'. The ports of the valve can be selectively connected in pairs under the control of the controller 1 to achieve the following: the first fluid power source 31 provides power to draw samples; the first fluid power source 31 and / or the second fluid power source 39 provides power to draw cleaning fluid; the first fluid power source 31 provides power to clean the detection chamber 33; and the second fluid power source 39 provides power to discharge cleaning fluid to clean the sampling needle a.

[0221] In this embodiment, controller 1 controls optical path subsystem 2, liquid path subsystem 3, particle confinement device 4, and drive subsystem 5 to detect one or more particles, including the following steps:

[0222] Preprocessing step S1.11: This step is the same as the preprocessing step in Example 7, so it will not be described again.

[0223] In the sampling step S2.11, the controller 1 controls the first gate device 34 to connect the inlet of the detection chamber 33 with the sampling needle a, and controls the drive device to position the sampling needle a at the sampling position. Then, the controller controls the first fluid power source 31 to provide power so that the sampling needle a draws the sample from the sample container 36 placed on the sampling position b. In this embodiment, the first fluid power source 31 transports the drawn sample to the detection chamber 33. The specific process is the same as the sampling step in Embodiment Seven, and therefore will not be described in detail.

[0224] In detection step S3.11, controller 1 controls the detection device to detect the light signal of particle f in the detection chamber. Similarly, this step is the same as the detection step in Example 1, and therefore will not be described again.

[0225] After cleaning step S4.11 and detection, the controller 1 closes the particle confinement device 4 or controls the particle confinement device 4 away from the detection chamber 33, so that the particles in the detection chamber 33 are not confined by the particle confinement device 4, facilitating particle discharge. Then, the detection chamber 33 can be cleaned. The cleaning of the detection chamber 33 is powered by the first fluid power source 31. Similar to the previous embodiment, for cleaning the sampling needle a, the controller 1 controls the drive device to position the sampling needle a at the waste liquid level c, controls the first gate device 34 to connect the sampling needle a and the second fluid power source 39, and controls the second fluid power source 39 to provide power to discharge the cleaning liquid through the sampling needle a to the first waste liquid recovery device 38 to clean the sampling needle a. This process can be carried out in the sampling step, the detection step, or the cleaning step. The aspiration and discharge of the cleaning liquid can also be carried out in different steps, as long as they are carried out after the sampling is completed. This embodiment does not limit this.

[0226] In the above embodiments, the sampling position b and the waste liquid discharge position c can be different work positions. For example, the drive subsystem 5 also includes a tray, on which one position is used to place the sample container 36, which is the sampling position b, and another position is used to place the waste liquid recovery device 38, which is the waste liquid discharge position c. The drive device moves the sampling needle a and / or the tray so that the sampling needle a is located at the sampling position when sampling, and at the waste liquid discharge position when discharging waste liquid. Of course, the sampling position b and the waste liquid discharge position c can also be the same station. For example, the drive subsystem 5 also includes a tray, on which one position is used to place both the sample container 36 and the waste liquid recovery device 38. When the sample container 36 is placed at this position, it is the sampling position b, and when the waste liquid recovery device 38 is placed at this position, it is the waste liquid discharge position c. Alternatively, the sampling position b and the waste liquid discharge position c can be at the same coordinate position. When a sample needs to be collected, the sampling needle and / or the sample container is moved to this position to collect the sample. When waste liquid needs to be discharged, the sampling needle and / or the waste liquid container is moved to this position to discharge the waste liquid. In practice, the sample container 36 can also be used as the waste liquid recovery device 38 to receive waste liquid after sampling. The liquid suction position d and the waste liquid discharge position c can be different stations. For example, the drive subsystem 5 also includes a tray, on which one position is used to place the cleaning fluid container 37 (liquid suction position d), and another position is used to place the waste liquid recovery device 38 (waste liquid discharge position c). Of course, the suction position d and the waste liquid discharge position c can also be the same station. For example, the drive subsystem 5 also includes a tray, on which a position is used to place both the cleaning fluid container 37 and the waste liquid recovery device 38. When the cleaning fluid container 37 is placed at this position, it is the suction position d, and when the waste liquid recovery device 38 is placed at this position, it is the waste liquid discharge position c. Alternatively, the suction position d and the waste liquid discharge position c can be at the same coordinate position. When cleaning fluid needs to be drawn, the sampling needle and / or the cleaning fluid container is moved to this position to collect the cleaning fluid. When waste liquid needs to be discharged, the sampling needle and / or the waste liquid container is moved to this position to discharge the waste liquid. In practice, after drawing cleaning fluid, the cleaning fluid container 37 can also be used as a waste liquid recovery device 38 to receive waste liquid.

[0227] The sampling position b and the liquid aspiration position d can be different positions. For example, the drive subsystem 5 also includes a tray, on which one position is used to place the sample container 36 (sampling position b), and another position is used to place the cleaning fluid container 37 (liquid aspiration position d). The drive device moves the sampling needle a and / or the tray so that the sampling needle a is located at the sampling position when aspirating samples, and at the liquid aspiration position when aspirating cleaning fluid. Of course, the sampling position b and the liquid aspiration position d can also be the same position. For example, the drive subsystem 5 also includes a tray, on which one position is used to place both the sample container 36 and the cleaning fluid container 37. When the sample container 36 is placed at this position, it is the sampling position b, and when the cleaning fluid container 37 is placed at this position, it is the liquid aspiration position d. Or, for example, the sampling position b and the liquid aspiration position d are at the same coordinate position. When a sample needs to be aspirated, the sampling needle and / or the sample container are moved to this position to collect the sample. When a cleaning fluid needs to be aspirated, the sampling needle and / or the waste liquid container are moved to this position to aspirate the cleaning fluid.

[0228] In summary, this invention can both directly deliver samples to the testing chamber via the sampling needle and discharge cleaning fluid via the sampling needle. It is evident that the design of the liquid path subsystem simplifies the sample delivery process, improves testing efficiency, and simultaneously cleans the sampling needle, reducing interference from sample residues in subsequent tests.

[0229] The above examples illustrate the present invention only to aid in understanding it and are not intended to limit the scope of the invention. Those skilled in the art can make various simple deductions, modifications, or substitutions based on the principles of this invention.

Claims

1. A detection system for analyzing one or more particles, characterized in that, include: A detection chamber is a place for detecting one or more particles to be analyzed. A first fluid power source used to power the flow of liquid in a fluid circuit; A sampling needle is used to draw a sample using power provided by a first fluid power source, and the sampling needle is connected to the first fluid power source through the detection chamber; A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves. The detection device is used to detect the optical signals of particles within the detection chamber; Controller, used for: Control the operation of the first fluid power source to draw in cleaning fluid through the fluid path; During sampling, the sampling needle is connected to the first fluid power source through the detection chamber, and the first fluid power source is controlled to provide power to make the sampling needle draw up the sample and transport the drawn up sample to the detection chamber; The light source and detection device are controlled to detect the samples in the detection chamber; During cleaning, the sampling needle is connected to the first fluid power source through the detection chamber. The first fluid power source is controlled to provide power, and the cleaning fluid from at least one section from the first fluid power source to the outlet of the detection chamber, the sample in the detection chamber, and the liquid from the detection chamber to the sampling needle are discharged through the sampling needle to clean the fluid path. In this process, the cleaning fluid from at least one section from the first fluid power source to the outlet of the detection chamber first passes through the detection chamber to clean the detection chamber, and then is discharged from the sampling needle to clean the sampling needle.

2. A detection system for analyzing one or more particles, characterized in that, include: A sampling position is used to provide a sample to be analyzed, the sample containing one or more particles to be analyzed; A detection chamber is a place for detecting one or more particles to be analyzed. Wastewater discharge level, used to receive wastewater; A first fluid power source used to power the flow of liquid in a fluid circuit; A sampling needle is used to draw a sample at the sampling position by relying on the power provided by a first fluid power source, and the sampling needle is connected to the first fluid power source through the detection chamber; A driving device for driving the sampling needle and / or the aspiration position to move so that the sampling needle is located at the aspiration position when aspiration is performed, and for driving the sampling needle and / or the waste liquid position to move so that the sampling needle is located at the waste liquid position when waste liquid is discharged; A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves. The detection device is used to detect the optical signals of particles within the detection chamber; Controller, used for: Control the operation of the first fluid power source to draw in cleaning fluid through the fluid path; The first fluid power source is controlled to operate and the drive device is controlled to drive. When the drive device is controlled to position the sampling needle at the sampling position, the sampling needle is connected to the first fluid power source through the detection chamber. The first fluid power source is controlled to provide power so that the sampling needle can pick up the sample at the sampling position and transport the picked-up sample to the detection chamber. The light source and detection device are controlled to detect the samples in the detection chamber; The control drive device positions the sampling needle at the waste liquid level. The sampling needle is connected to the first fluid power source through the detection chamber. The control of the first fluid power source provides power to discharge the cleaning fluid from at least one section from the first fluid power source to the outlet of the detection chamber, the sample in the detection chamber, and the liquid from the detection chamber to the sampling needle through the sampling needle to clean the liquid path. In this process, the cleaning fluid from at least one section from the first fluid power source to the outlet of the detection chamber first passes through the detection chamber to clean the detection chamber, and then is discharged from the sampling needle to clean the sampling needle.

3. The detection system as described in claim 1 or 2, characterized in that, The first fluid power source is connected to the outlet of the detection chamber via a first pipe, and the sampling needle is connected to the inlet of the detection chamber; the cleaning fluid, comprising at least one section from the first fluid power source to the outlet of the detection chamber, includes: Cleaning fluid from the first fluid power source to at least one section of the first pipeline.

4. The detection system as described in claim 1 or 2, characterized in that, Also includes: A first gating device having an outlet, a first inlet, and a second inlet is used to connect the outlet and the first inlet via a switching mechanism. After switching, the outlet is connected to the second inlet; the outlet of the first gate device is connected to the inlet of the detection chamber, the first inlet of the first gate device is connected to the sampling needle, and the second inlet of the first gate device is connected to the cleaning fluid container for providing cleaning fluid through the second pipe; the first fluid power source is connected to the outlet of the detection chamber.

5. The detection system as described in claim 1, 2, or 4, characterized in that, Also includes: The second gate device; the first fluid power source is connected to the outlet of the detection chamber through the second gate device, and the second gate device is also connected to a cleaning fluid container for providing cleaning fluid through a third pipeline; The second gating device is used to connect at least one of the third pipeline and the testing chamber to the first fluid power source.

6. The detection system as described in claim 1 or 2, characterized in that, Also includes: A second gate device with a drain outlet is provided, and a first fluid power source is connected to the outlet of the detection chamber through the second gate device. The drain outlet is used to discharge liquid toward the waste liquid recovery device. The second gating device is used to connect the drain outlet to the outlet of the detection chamber via switching, or to connect the drain outlet to the first fluid power source via switching; and to connect the outlet of the detection chamber to the first fluid power source via switching. The second fluid power source is used to provide power for the flow of liquid in the fluid circuit; A first gating device having an outlet, a first inlet, a second inlet, and a third inlet, for connecting the outlet to the second inlet via a switch, and / or connecting the second inlet to the third inlet via a switch; connecting the outlet to the first inlet via a switch, and connecting the outlet to the third inlet via a switch; The outlet of the first gate device is connected to the inlet of the detection chamber, the first inlet of the first gate device is connected to the sampling needle, the second inlet of the first gate device is connected to a cleaning fluid container for providing cleaning fluid through a second pipe, and the third inlet of the first gate device is connected to a second fluid power source.

7. The detection system as described in claim 2, characterized in that, Also includes: The suction position is used to place the cleaning solution container to supply the cleaning solution; The driving device is also used to drive the sampling needle and / or the liquid aspiration position to move so that the sampling needle is located at the liquid aspiration position when aspirating the cleaning liquid.

8. The detection system as described in claim 1 or 7, characterized in that, Also includes: The second gate device connects the first fluid power source to the outlet of the detection chamber through the second gate device, and the second gate device is also connected to a cleaning fluid container for providing cleaning fluid through a third pipeline. The second gating device is used to connect at least one of the third pipeline and the testing chamber to the first fluid power source.

9. The detection system as described in claim 1 or 7, characterized in that, Also includes: A second gate device with a drain outlet is provided, and a first fluid power source is connected to the outlet of the detection chamber through the second gate device. The drain outlet is used to discharge liquid toward the waste liquid recovery device. The second gating device is used to connect the drain outlet to the outlet of the detection chamber via switching, or to connect the drain outlet to the first fluid power source via switching; and to connect the outlet of the detection chamber to the first fluid power source via switching. The second fluid power source is used to provide power for the flow of liquid in the fluid circuit; A first gating device having an outlet, a first inlet, and a second inlet; the outlet of the first gating device is connected to the inlet of the detection chamber, the first inlet of the first gating device is connected to a sampling needle, and the second inlet of the first gating device is connected to a second fluid power source; The first gating device is at least used to connect the outlet to the second inlet by switching, and to connect the outlet to the first inlet by switching; or, the outlet of the first gating device is connected to its first inlet, and the first gating device is used to control the connection and disconnection between its outlet and its second inlet; or, the outlet of the first gating device is connected to its second inlet, and the first gating device is used to control the connection and disconnection between its outlet and its first inlet.

10. A detection system for analyzing one or more particles, characterized in that, include: A detection chamber is a place for detecting one or more particles to be analyzed. A first fluid power source is used to provide power for the flow of liquid in the liquid circuit, and the first fluid power source is connected to the outlet of the detection chamber; A second fluid power source used to power the flow of liquid in a fluid circuit; A sampling needle, used to draw samples using power provided by a first fluid power source; The first gating device is used to select the connection, and the entrance to the detection chamber and the second fluid power source are both connected to the sampling needle through the first gating device; A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves. The detection device is used to detect the optical signals of particles within the detection chamber; Controller, used for: Control the operation of the first or second fluid power source to draw cleaning fluid into the fluid path; The system controls the first gating device to select the sample and the first fluid power source to operate. The first gating device connects the sampling needle to the inlet of the detection chamber. The system controls the first fluid power source to provide power so that the sampling needle can draw up the sample. The system also controls the first fluid power source to transport the sample drawn up into the liquid path to the detection chamber. The light source and detection device are controlled to detect the samples in the detection chamber; After sampling is completed, the sampling needle and the second fluid power source are connected through the first gating device, and the second fluid power source is controlled to provide power to discharge the cleaning fluid through the sampling needle to clean the sampling needle. After the test is completed, the second fluid power source and the test chamber are connected through the first gate device. The second fluid power source is controlled to provide power to discharge another part of the cleaning fluid through the first gate device and the test chamber in sequence, so as to clean the test chamber. Alternatively, the sampling needle and the inlet of the detection chamber can be connected by the first gating device, and the first fluid power source can be controlled to provide power to discharge the cleaning fluid through the detection chamber and the first gating device in sequence, so as to clean the detection chamber.

11. A detection system for analyzing one or more particles, characterized in that, include: A sampling position is used to provide a sample to be analyzed, the sample containing one or more particles to be analyzed; A detection chamber is a place for detecting one or more particles to be analyzed. Wastewater discharge level, used to receive wastewater; A first fluid power source is used to provide power for the flow of liquid in the liquid circuit, and the first fluid power source is connected to the outlet of the detection chamber; A second fluid power source used to power the flow of liquid in a fluid circuit; A sampling needle is used to draw a sample using power provided by a first fluid power source at the sampling position; A driving device for driving the sampling needle and / or the aspiration position to move so that the sampling needle is located at the aspiration position when aspiration is performed, and for driving the sampling needle and / or the waste liquid position to move so that the sampling needle is located at the waste liquid position when waste liquid is discharged; The first gating device is used to select the connection, and the entrance to the detection chamber and the second fluid power source are both connected to the sampling needle through the first gating device; A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves. The detection device is used to detect the optical signals of particles within the detection chamber; Controller, used for: Control the operation of the first or second fluid power source to draw cleaning fluid into the fluid path; The system controls the first gating device to select, the first fluid power source to operate, and the drive device to drive. When the drive device controls the sampling needle to be in the sampling position, the first gating device connects the sampling needle to the inlet of the detection chamber. The system controls the first fluid power source to provide power so that the sampling needle can pick up the sample in the sampling position, and controls the first fluid power source to transport the sample picked up in the liquid path to the detection chamber. The light source and detection device are controlled to detect the samples in the detection chamber; After sampling is completed, the control drive device moves the sampling needle to the waste liquid level, and the first gate device connects the sampling needle to the second fluid power source. The second fluid power source is controlled to provide power to discharge the cleaning liquid through the sampling needle to clean the sampling needle. After the test is completed, the second fluid power source and the test chamber are connected through the first gate device. The second fluid power source is controlled to provide power to discharge another part of the cleaning fluid through the first gate device and the test chamber in sequence, so as to clean the test chamber. Alternatively, the sampling needle and the inlet of the detection chamber can be connected by the first gating device, and the first fluid power source can be controlled to provide power to discharge the cleaning fluid through the detection chamber and the first gating device in sequence, so as to clean the detection chamber.

12. The detection system as described in claim 10 or 11, characterized in that... It also includes: a second gate device with a drain outlet; a first fluid power source connected to the outlet of the detection chamber via the second gate device, the drain outlet being used to drain liquid toward the waste liquid recovery device; the second gate device being used to connect the drain outlet to the outlet of the detection chamber via switching, or to connect the drain outlet to the first fluid power source via switching; and to connect the outlet of the detection chamber to the first fluid power source via switching.

13. The detection system as described in claim 12, characterized in that, The first gate device includes an outlet, a first inlet, a second inlet, and a third inlet, configured to connect the outlet to the first inlet via a switching mechanism; connect the first inlet to the third inlet via a switching mechanism; and further configured to connect the outlet to the third inlet via a switching mechanism and connect the second inlet to the third inlet via a switching mechanism, or connect the outlet to the third inlet via a switching mechanism and connect the outlet to the second inlet via a switching mechanism, or connect the outlet to the second inlet via a switching mechanism and connect the second inlet to the third inlet via a switching mechanism; the outlet of the first gate device is connected to the inlet of the detection chamber, the first inlet of the first gate device is connected to a sampling needle, the second inlet of the first gate device is connected to a cleaning fluid container for providing cleaning fluid via a second pipe, and the third inlet of the first gate device is connected to a second fluid power source; or... The first gating device includes an outlet, a first inlet, and a second inlet, and is at least used to connect the first inlet and the second inlet by switching, and to connect the outlet and the first inlet by switching; the outlet of the first gating device is connected to the inlet of the detection chamber, the first inlet of the first gating device is connected to the sampling needle, and the second inlet of the first gating device is connected to the second fluid power source.

14. The detection system as described in claim 11, characterized in that... Also includes: The suction position is used to place the cleaning solution container to supply the cleaning solution; The driving device is also used to drive the sampling needle and / or the liquid aspiration position to move so that the sampling needle is located at the liquid aspiration position when aspirating the cleaning liquid.

15. The detection system as described in claim 10, 11, or 14, characterized in that, The first gate device also has a drain port for discharging waste liquid.

16. The detection system as described in claim 2 or 11, characterized in that, The sampling position and the waste liquid discharge position are either different positions or the same position.

17. A detection system for analyzing one or more particles, characterized in that, include: A detection chamber is a place for detecting one or more particles to be analyzed. A first fluid power source is used to provide power for the flow of liquid in the liquid circuit, and the first fluid power source is connected to the outlet of the detection chamber; A second fluid power source used to power the flow of liquid in a fluid circuit; A sampling needle, used to draw samples using power provided by a first fluid power source; A three-way device having a first interface, a second interface, and a third interface, wherein the first interface, the second interface, and the third interface are interconnected; the first interface of the three-way device is connected to the entrance of the detection chamber, the second interface of the three-way device is connected to the sampling needle, and the third interface of the three-way device is connected to a second fluid power source; A light source is used to illuminate the particles in the detection chamber so that the particles emit light signals related to the characteristics of the particles themselves. The detection device is used to detect the optical signals of particles within the detection chamber; Controller, used for: Control the operation of the first or second fluid power source to draw cleaning fluid into the fluid path; The first fluid power source is controlled to provide power to make the sampling needle draw up the sample, and the first fluid power source is controlled to transport the sample drawn into the liquid path to the detection chamber; The light source and detection device are controlled to detect the samples in the detection chamber; After sampling is completed, the second fluid power source is controlled to provide power to discharge the cleaning solution through the sampling needle to clean the sampling needle; After the test is completed, the second fluid power source is controlled to provide power to discharge another part of the cleaning fluid through the three-way device and the test chamber in sequence, so as to clean the test chamber; or, the first fluid power source is controlled to provide power to discharge the cleaning fluid through the test chamber and the three-way device in sequence, so as to clean the test chamber.

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