Device and method for collecting seed sphere and rhizosphere soil in sections and layers

Abstract

The present invention provides a device and method for collecting seed and rhizosphere soil in sections and layers, the device comprising a soil sampling ring and a center point positioning frame; a center hole is arranged at the center of the center point positioning frame, and the center hole is located at the center of the soil sampling ring; the soil sampling ring is cylindrical, and the bottom circumference is the ring blade. The device for collecting seed and rhizosphere soil in sections and layers provided by the present invention is simple and easy to operate. The method provided by the present invention can realize the accurate collection of seed and rhizosphere soil, thereby providing necessary guarantee for subsequent research.

Description

Technical Field

[0001] The invention relates to the technical field of soil collection, and in particular to a device and method for collecting seed sphere and rhizosphere soil in sections and layers. Background Art

[0002] Soil is crucial to the seed germination process and plant growth. The physical and chemical properties, nutrient content, and microbial system of soil are the main indicators for measuring the impact of soil on plants, and the accurate collection of soil samples determines the smooth progress of research work and the accuracy of the results. Existing sampling of seed and rhizosphere soil mostly uses soil drills, root drills and other soil sampling devices to take multi-point samples of soil at different depths. The selection of sampling points is not accurate enough, and the composition and nutrients of the soil are unevenly distributed. Multi-point sampling may also have problems such as poor sample representativeness. The collection of a large number of samples improves the accuracy of the measurement results, but it requires digging a larger operating profile, and the sampling radius, depth and other data need to be measured multiple times during the collection process, which is time-consuming and labor-intensive.

[0003] For example, in the production and application of seed treatment agents such as seed dressing agents and granules, studying the release, migration, and adsorption of chemical agents in the soil is the basis for clarifying the control effect of the agents. The regularity problem can be quantitatively analyzed by measuring the content of effective ingredients in the preparations in different layers of soil. However, chemical agents are unevenly distributed in the soil and have a low content. How to achieve accurate and effective collection of soil samples through innovative devices and methods is a common technical problem in studying the efficient use of exogenous substances such as pesticides and fertilizers on crops. Summary of the invention

[0004] The purpose of the present invention is to solve the defects of the above-mentioned prior art and provide a device and method for collecting soil from the seed sphere and rhizosphere of plants.

[0005] A device for collecting soil from the seed and rhizosphere in sections and layers, comprising a soil collecting ring and a center point positioning frame; a center hole is arranged at the center of the center point positioning frame, and the center hole is located at the center of the soil collecting ring;

[0006] The soil-taking ring cutter is cylindrical, and the bottom circumference is the ring cutter edge.

[0007] Furthermore, in the device for collecting seed sphere and rhizosphere soil in sections and layers as described above, the radius and height of the soil sampling ring knife are consistent.

[0008] Furthermore, in the device for collecting seed sphere and rhizosphere soil in sections and layers as described above, the center point positioning frame adopts a cross bracket, and the center hole is located at the center of the cross.

[0009] A method for collecting seed sphere and rhizosphere soil in sections and layers, comprising the following steps:

[0010] (1) The seed sowing position or plant stem is used as the sampling center point, and the cutting center point marker is placed at the sampling center point;

[0011] (2) Select a soil sampling ring that meets the specifications, place a matching center point positioning frame of the same specifications in the middle of the soil sampling ring, make the center hole of the positioning frame pass through the center point marker, and press the soil sampling ring and the center point positioning frame vertically into the soil until the soil sample is slightly higher than the soil sampling ring;

[0012] (3) removing the center point positioning frame;

[0013] (4) reducing the radius of the soil cutting ring, placing the center point positioning frame of the same specification in the middle of the soil cutting ring with the reduced radius, and pressing them vertically into the soil together, and then removing the center point positioning frame again;

[0014] (5) gradually reducing the radius of the soil sampling ring knife, and vertically pressing soil sampling ring knives of different specifications together with the matching center point positioning frame into the soil to collect soil samples in turn; during each sampling process, ensure that the center point marker accurately passes through the center hole of the center point positioning frame;

[0015] (6) Remove all the ring cutters completely, and ensure that the soil inside the ring cutters is not disturbed during the removal process. If there is a lack or looseness, discard the collected soil sample and collect it again;

[0016] (7) Collect the annular soil samples formed by the soil ring knife vertically pressing into the soil twice;

[0017] (8) The soil samples collected each time were placed in ziplock bags for preservation, numbering and testing.

[0018] Furthermore, in the above-mentioned method for collecting soil from the seed sphere and rhizosphere of plants, when collecting soil samples in step (7), the soil at the top and bottom of the soil sampling ring is flattened, and then the soil samples inside and at the bottom of each two-layer ring are completely collected from the outside to the inside.

[0019] Furthermore, in the above-mentioned method for collecting soil from the seed sphere and rhizosphere of a plant, the center point marker is made of hard thin steel wire.

[0020] Furthermore, in the above-mentioned method for collecting soil from the seed sphere and rhizosphere of a plant, the order of collecting in step (7) is: first collect the outer circle with a larger radius, one ring at a time, and gradually collect inward until all the collection is completed.

[0021] Beneficial effects:

[0022] The invention provides a device for collecting seed sphere and rhizosphere soil in sections and layers, and provides a complete method for using the device. The device is simple and easy to operate. The method provided by the invention can achieve accurate collection of seed sphere and rhizosphere soil, thereby providing necessary guarantee for subsequent research.

[0023] The stratification refers to different soil depths in the vertical direction, and the segmentation refers to different distances in the horizontal direction with the plant as the center. Both segmentation and stratification are achieved by using different diameters of ring cutters. The reason why the radius is designed to be consistent with the height is that the growth of plant roots or the pesticides and fertilizers added to the outer edge are all centered on the root or application point and spread in all directions. Therefore, the ring cutter is designed to have a radius consistent with the height, and the soil sample obtained is closer to the growth or material distribution law. Therefore, the center point of the seed position or root mark should be marked.

[0024] The function of the positioning frame is to ensure the fixation of the center or point, similar to concentric circles.

[0025] The method provided by the present invention can reduce the influence of soil expansion caused by the pressing of the ring knife by gradually reducing the sampling radius, thereby facilitating sampling.

[0026] The method provided by the present invention selects a ring cutter that meets the specifications, places a matching positioning frame of the same specifications on the top of the ring cutter, and the center hole of the positioning frame and the ring cutter pass through the center point marker at the same time, thereby ensuring the accuracy of soil collection. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the soil cutting ring structure of the present invention;

[0028] Figure 2 for Figure 1 Top view of the . DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention is described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] like Figure 1 , Figure 2 As shown, the present invention provides a device for collecting seed sphere and rhizosphere soil in sections and layers, including a soil sampling ring 11 and a center point positioning frame 12; a center hole 121 is provided at the center of the center point positioning frame 12, and the center hole 121 is located at the center of the soil sampling ring 1; the soil sampling ring 1 is cylindrical, and its bottom circumference is the ring knife edge.

[0031] The center point positioning frame 12 is used to locate the sampling center point. The center hole diameter is fixed, and different sampling radii can be accurately and conveniently determined. The center hole diameter is fixed, which improves the accuracy and operability of the device.

[0032] Furthermore, the center point positioning frame 12 adopts a cross-shaped frame with a round hole in the center for easy observation and identification.

[0033] Furthermore, the radius of the soil sampling ring 1 is consistent with the height. During the plant-soil interaction process, substances or roots diffuse, migrate and distribute in the soil in both horizontal and vertical directions. The cylindrical ring is designed to have a radius consistent with the height, and the collected soil samples can accurately represent the characteristics of the seed and rhizosphere soils.

[0034] The present invention also provides a method for collecting seed sphere and rhizosphere soil in sections and layers, comprising the following steps:

[0035] (1) The seed sowing position or plant stem is used as the sampling center point, and the cutting center point marker is placed at the sampling center point;

[0036] (2) Select a soil sampling ring cutter 11 that meets the specifications, place a matching center point positioning frame 12 of the same specifications in the middle of the soil sampling ring cutter 11, make the center hole 121 of the positioning frame pass through the center point marker, and vertically press the soil sampling ring cutter 11 and the center point positioning frame 12 into the soil until the soil sample is slightly higher than the soil sampling ring cutter 11;

[0037] (3) removing the center point positioning frame 12;

[0038] (4) reducing the radius of the soil cutting ring 11, placing the center point positioning frame 12 of the same specification in the middle of the soil cutting ring 11 with the reduced radius, and pressing them vertically into the soil together, and then removing the center point positioning frame 12 again;

[0039] (5) gradually reducing the radius of the soil sampling ring knife 11, and vertically pressing the soil sampling ring knives 11 of different specifications together with the matching center point positioning frame 12 into the soil in turn to collect soil samples; during each sampling process, ensure that the center point marker accurately passes through the center hole 121 of the center point positioning frame 12;

[0040] (6) Remove all the ring cutters completely, and ensure that the soil inside the ring cutters is not disturbed during the removal process. If there is a lack or looseness, discard the collected soil sample and collect it again;

[0041] (7) Collect the annular soil samples formed by the soil ring knife 11 vertically pressing into the soil twice;

[0042] (8) The soil samples collected each time were placed in ziplock bags for preservation, numbering and testing.

[0043] Furthermore, when collecting soil samples in step (7), the soil on the top and bottom of the soil sampling ring 11 is flattened, and then the soil samples inside and at the bottom of each two-layer ring sandwich are completely collected from the outside to the inside.

[0044] Furthermore, the center point marker is made of hard thin steel wire.

[0045] Example:

[0046] The method for sampling the seed sphere and rhizosphere soil in sections and layers proposed in the embodiment of the present invention is:

[0047] (1) The seed sowing position or plant stem is used as the sampling center point, and the center point is fixed with a hard thin steel wire or other easily available objects as a center point marker;

[0048] (2) According to the maximum sampling radius of the experimental design, select a ring knife that meets the specifications, place a matching positioning frame of the same specifications on the top of the ring knife, and let the center hole of the positioning frame and the ring knife pass through the center point marker at the same time, and fall smoothly and accurately to the sampling plane; with the cutting edge of the ring knife facing downward, use the ring knife handle or rubber hammer to press the ring knife vertically until the soil sample is slightly higher than the ring knife;

[0049] (3) In order to reduce the outward expansion effect of the ring knife on the sampled soil, the sampling radius is gradually reduced. During the process, the center holes of the positioning frames with different radii and the ring knives are ensured to accurately pass through the center point markers, and the ring knives of different specifications are pressed vertically into the soil in turn;

[0050] (4) When removing the ring cutter, carefully remove the soil on the outer wall of the ring cutter with the largest radius, and allow the soil at the bottom to slightly overflow the bottom of the ring cutter. Completely remove all the ring cutters, and ensure that the soil inside the ring cutter is not disturbed during the process. If there is a lack or looseness, discard the collected soil sample and collect it again;

[0051] (5) When collecting soil samples, flatten the soil at the top and bottom of the knife rings, and then collect the soil samples between each layer of knife rings and at the bottom from the outside to the inside. Collect the soil samples in sections and layers, and put all or a suitable amount of soil into self-sealing bags for preservation, numbering and testing.

[0052] Experimental example:

[0053] Collection of soil around seeds after cotton seeds were coated with seed coating agent

[0054] The purpose of the experiment is to coat cotton seeds with imidacloprid seed coating agent and sow them in pots (radius>10cm) or in the field. During the seed germination period, soil samples at different horizontal positions and depths around the seeds are collected, and the content of imidacloprid around the seeds and in the soil is determined by liquid chromatography-mass spectrometry to clarify its release and distribution patterns.

[0055] Method for collecting soil samples around seeds: Use circular cutters with radii of 2cm, 4cm, 6cm, 8cm and 10cm for sampling.

[0056] (1) Place a center point positioning frame with a radius of 10 cm on a ring cutter with a radius of 10 cm, and mark the seed position with a thin steel wire passing through the center hole of the positioning frame. At the same time, press the ring cutter vertically into the soil, apply force evenly, and avoid the ring cutter tilting; finally, remove the positioning frame;

[0057] (2) Place a positioning frame with a radius of 8 cm on a circular knife with a radius of 8 cm, pass a thin steel wire through the center hole of the positioning frame, and slowly drop it down to ensure that the sampling range of 8 cm is accurately positioned, press the circular knife vertically into the soil, and remove the positioning frame again;

[0058] (3) According to the above steps (1) and (2), 6 cm, 4 cm and 2 cm sampling rings are pressed into the soil in turn, and the soil at different levels and depths is fixed in sections and layers step by step;

[0059] (4) Remove excess soil from the outer wall of the 10 cm radius ring cutter, remove the entire ring cutter set at a depth of >10 cm, flatten the soil samples at both ends of the ring cutter, and then take out the soil samples of each section and layer in turn. After shrinking, retain 300 g of soil and put it into a ziplock bag and number it for testing.

[0060] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A device for collecting seed sphere and rhizosphere soil in sections and layers, characterized in that: It comprises a soil cutting ring (11) and a center point positioning frame (12); a center hole (121) is arranged at the center of the center point positioning frame (12), and the center hole (121) is located at the center of the soil cutting ring (11); The soil-taking ring cutter (11) is cylindrical, and the bottom circumference is the ring cutter edge; The radius and height of the soil-taking ring cutter (11) are consistent; The center point positioning frame (12) adopts a cross bracket, and the center hole (121) is located at the center of the cross; The method for collecting seed sphere and rhizosphere soil in sections and layers using the device comprises the following steps: (1) The seed sowing position or plant stem is used as the sampling center point, and the cutting center point marker is placed at the sampling center point; (2) Selecting a soil sampling ring (11) that meets the specifications, placing a matching center point positioning frame (12) of the same specifications in the middle of the soil sampling ring (11), allowing the center hole (121) of the positioning frame to pass through the center point marker, and vertically pressing the soil sampling ring (11) and the center point positioning frame (12) into the soil until the soil sample is slightly higher than the soil sampling ring (11); (3) removing the center point positioning frame (12); (4) reducing the radius of the soil-taking ring cutter (11), placing a matching center point positioning frame (12) of the same specification in the middle of the soil-taking ring cutter (11) with a reduced radius, and vertically pressing them into the soil together, and then removing the center point positioning frame (12) again; (5) gradually reducing the radius of the soil sampling ring knife (11), and sequentially vertically pressing soil sampling ring knives (11) of different specifications together with the matching center point positioning frame (12) into the soil to collect soil samples; during each sampling process, ensuring that the center point marker accurately passes through the center hole (121) of the center point positioning frame (12); (6) Remove all the ring cutters completely, and ensure that the soil inside the ring cutters is not disturbed during the removal process. If there is a lack or looseness, discard the collected soil sample and collect it again; (7) collecting the annular soil samples formed by the soil sampling ring knife (11) vertically pressing into the soil twice; (8) The soil samples collected each time were placed in ziplock bags for storage, numbering and testing; The order of collection in step (7) is: first collect the outer circle with a larger radius, one ring at a time, and gradually collect inward until all the collection is completed; When collecting soil samples in step (7), the soil on the top and bottom of the soil sampling ring (11) is flattened, and then the soil samples inside and at the bottom of each two-layer ring sandwich are completely collected from the outside to the inside.

2. The device for collecting seed sphere and rhizosphere soil in sections and layers according to claim 1, characterized in that: The center point marker is made of hard thin steel wire.

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