A biological cell staining device and its usage method

Abstract

The present invention discloses a biological cell staining device and a method for using the same, including a workbench. A rotating mechanism is arranged in the middle of the top of the workbench. A clamping mechanism is arranged on the top of the rotating mechanism. A hydraulic telescopic rod is fixedly connected to the right side of the top of the workbench. The top of the hydraulic telescopic rod is fixedly connected to a connecting plate. A rotating disc is fixedly connected to the bottom of the connecting plate. A plurality of injection heads are symmetrically and fixedly sleeved on the surface of the rotating disc. Outer sealing plates are symmetrically and movably sleeved on the surface of the workbench. A control console is fixedly connected to the left side of the top of the workbench. The present invention relates to the technical field of biological cells. With this biological cell staining device and the method for using the same, through the back-and-forth operations of tilting and resetting of the clamping mechanism, the staining of biological cells can be accelerated, solving the problem that although the rotation method can make them evenly mixed, the rotation time is relatively long and it is impossible to shorten the mixing of the staining agent and biological cells.

Description

Technical Field

[0001] The present invention relates to the technical field of biological cell technology, and particularly to a biological cell staining device and a using method thereof. Background Art

[0002] The most important and basic feature of organisms is that they carry out metabolism and inheritance. All organisms must possess anabolic metabolism and catabolic metabolism, which are two opposite processes and can reproduce, which is the basis of life phenomena. The natural world is composed of biological and non-biological substances and energy. Organisms with life characteristics are called living organisms, and non-living things including substances and energy are called non-biological.

[0003] After the existing biological cells are dripped with a staining agent, the device is rotated to uniformly mix the staining agent with the biological cells. However, although the uniform mixing can be achieved by rotation alone, the rotation time is relatively long, and it is impossible to shorten the mixing of the staining agent and the biological cells. Summary of the Invention

[0004] (I) Technical Problems to be Solved

[0005] Aiming at the deficiencies of the prior art, the present invention provides a biological cell staining device and a using method thereof, which solve the problem that although the uniform mixing can be achieved by rotation, the rotation time is relatively long and it is impossible to shorten the mixing of the staining agent and the biological cells.

[0006] (II) Technical Solutions

[0007] To achieve the above object, the present invention is realized through the following technical solutions: A biological cell staining device includes a workbench, a rotating mechanism is arranged in the middle of the top of the workbench, a clamping mechanism is arranged on the top of the rotating mechanism, a hydraulic telescopic rod is fixedly connected to the right side of the top of the workbench, a connecting plate is fixedly connected to the top of the hydraulic telescopic rod, a rotating disk is fixedly connected to the bottom of the connecting plate, and a plurality of injection heads are symmetrically and fixedly sleeved on the surface of the rotating disk.

[0008] The rotating mechanism includes a fixed disk and a rotating rod. Second bearings and first bearings are symmetrically and fixedly sleeved on the surface of the rotating rod. Pin columns are symmetrically and fixedly connected to the surface of the first bearing. A cylinder is rotatably sleeved on the surface of the second bearing through a rotating component. The bottom of the rotating rod is fixedly connected to a servo motor, and a sleeve plate is fixedly sleeved on the surface of the servo motor.

[0009] The clamping mechanism includes an upper cover and a lower cover. Threaded sleeves are provided on the surfaces of both the upper cover and the lower cover. A plurality of placement grooves are symmetrically and fixedly formed on the surfaces of the upper cover and the lower cover. A plurality of sliding grooves are symmetrically and fixedly formed on the top of the lower cover. A plurality of chutes are symmetrically and fixedly formed on the inner walls of the plurality of sliding grooves. A plurality of pressing rods are slidably sleeved in the inner cavities of the plurality of sliding grooves. Sliders are symmetrically and fixedly connected to the surfaces of the pressing rods. Springs are fixedly connected between the surfaces of the sliders and the inner walls of the chutes.

[0010] Preferably, outer sealing plates are symmetrically and movably sleeved on the surface of the workbench, and a control console is fixedly connected to the left side of the top of the workbench.

[0011] Preferably, the top of the rotating rod is fixedly installed on the top of the lower cover through a mounting plate, and the fixed plate is fixedly connected to the top of the workbench.

[0012] Preferably, the rotating assembly is fixedly connected to the inner wall of the fixed plate, and one ends of the pin and the sleeve plate are rotatably sleeved on the inner wall of the workbench.

[0013] Preferably, one end of the sliding groove communicates with the inner cavity of the placement groove, and one end of the pressing rod fits with the inner wall of the placement groove.

[0014] Preferably, the upper cover and the lower cover are symmetrically and fixedly installed through bolts, and the surface of the slider is slidably sleeved on the inner wall of the chute.

[0015] Preferably, the bottom of the screw rod contacts the other end of the pressing rod, and the center line of the placement groove is aligned with that of the injection head.

[0016] The present invention also discloses a method for using a biological cell staining device, which specifically includes the following steps:

[0017] S1. Clamping: First, place the test tubes containing biological cells in the inner cavities of the placement grooves in sequence. Then rotate the screw rod so that the screw rod moves downward on the surfaces of the upper cover and the lower cover, making the bottom of the screw rod abut against the opposite end of the pressing rod and pressing the pressing rod, causing the pressing rod to move to one side in the inner cavity of the sliding groove, and making one end of the pressing rod extend to clamp the surface of the test tube in the inner cavity of the placement groove opened on the surface of the lower cover. At the same time, the slider will also slide in the inner cavity of the chute as the pressing rod moves, and the spring will be compressed. Additionally, when it is necessary to take out, move the screw rod upward so that the screw rod leaves one end of the pressing rod, and under the elastic force of the spring, it will drive the pressing rod to reset so that it no longer clamps the test tube, and then the test tube can be taken out.

[0018] S2. Inject the stain, and then start the hydraulic telescopic rod through the console to make the hydraulic telescopic rod lift and lower, so as to drive the rotating disk to descend through the connecting plate, so that multiple injection heads sleeved on the surface of the rotating disk are inserted into the inner cavity of the clamped test tube. Then inject the stain into the test tube, and then reset the hydraulic telescopic rod through the console to make the injection head extend out of the inner cavity of the test tube, and then cover the lids on the top of the test tube in sequence;

[0019] S3. Rotation. Finally, first start the servo motor on the surface of the sleeve plate through the console, so that the output end of the servo motor drives the rotating rod to rotate in the inner cavities of the first bearing and the second bearing. Then drive the clamping mechanism to rotate through the mounting plate. While rotating, start the cylinder in the inner cavity of the rotating assembly to expand and contract, so that the extending end of the cylinder is rotationally sleeved by the rotating assembly to push the second bearing to tilt to one side. At the same time, under the rotation of the pin and the sleeve plate, the rotating rod drives the clamping mechanism to tilt to 15 - 35 °C. Through the tilting and resetting operations of the clamping mechanism, the flow direction of the liquid in the test tube can be changed when the test tube rotates, and the staining of biological cells can be accelerated.

[0020] Beneficial effects

[0021] The present invention provides a biological cell staining device and its usage method. Compared with the prior art, it has the following beneficial effects:

[0022] 1. For this biological cell staining device and its usage method, by starting the servo motor on the surface of the sleeve plate, the output end of the servo motor drives the rotating rod to rotate in the inner cavities of the first bearing and the second bearing. Then drive the clamping mechanism to rotate through the mounting plate. While rotating, start the cylinder in the inner cavity of the rotating assembly to expand and contract, so that the extending end of the cylinder is rotationally sleeved by the rotating assembly to push the second bearing to tilt to one side. At the same time, under the rotation of the pin and the sleeve plate, the rotating rod drives the clamping mechanism to tilt to 15 - 35 °C. Through the tilting and resetting operations of the clamping mechanism, the flow direction of the liquid in the test tube can be changed when the test tube rotates, and the staining of biological cells can be accelerated.

[0023] 2. For this biological cell staining device and its usage method, by placing the test tubes containing biological cells in the inner cavity of the placement groove in sequence, and then rotating the lead screw, the lead screw moves downward on the surfaces of the upper cover and the lower cover, so that the bottom of the lead screw abuts against the opposite ends of the extrusion rods and extrudes the extrusion rods, causing the extrusion rods to move to one side in the inner cavity of the sliding groove, and one end of the extrusion rod extends out to clamp the surface of the test tube in the placement groove on the surface of the lower cover, completing simultaneous collective clamping and reducing the time for clamping one by one.

[0024] 3. The biological cell staining device and its usage method start the hydraulic telescopic rod through the console, causing the hydraulic telescopic rod to lift and lower. Thereby, the rotating disk is driven to descend through the connecting plate, enabling multiple injection heads sleeved on the surface of the rotating disk to insert into the inner cavity of the clamped test tube. Then, the staining agent is injected into the test tube. Subsequently, the hydraulic telescopic rod is reset through the console, causing the injection heads to extend from the inner cavity of the test tube. Then, the lids on the tops of the test tubes are covered in sequence to achieve the effect of simultaneous injection. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 is a schematic structural diagram of the present invention;

[0026] Figure 2 is a schematic diagram of the rotating mechanism of the structure of the present invention;

[0027] Figure 3 is a schematic diagram of the clamping mechanism of the structure of the present invention;

[0028] Figure 4 is an exploded view of the clamping mechanism of the structure of the present invention;

[0029] Figure 5 is the structure of the present invention Figure 4 partial enlarged view at A in the figure.

[0030] In the figure: 1, workbench; 2, outer sealing plate; 3, hydraulic telescopic rod; 4, connecting plate; 5, rotating disk; 6, injection head; 7, console; 8, rotating mechanism; 81, fixed disk; 82, rotating rod; 83, first bearing; 84, second bearing; 85, mounting disk; 86, pin; 87, servo motor; 88, sleeve plate; 89, rotating assembly; 810, cylinder; 9, clamping mechanism; 91, upper cover; 92, lower cover; 93, placement groove; 94, bolt; 95, lead screw; 96, slider; 97, chute; 98, extrusion rod; 99, spring; 910, sliding groove. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] The technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0032] Please refer to Figure 1, an embodiment of the present invention provides a technical solution: a biological cell staining device, including a workbench 1. A rotating mechanism 8 is arranged in the middle of the top of the workbench 1. A clamping mechanism 9 is arranged on the top of the rotating mechanism 8. A hydraulic telescopic rod 3 is fixedly connected to the right side of the top of the workbench 1. The top of the hydraulic telescopic rod 3 is fixedly connected to a connecting plate 4. A rotating disk 5 is fixedly connected to the bottom of the connecting plate 4. A plurality of injection heads 6 are symmetrically and fixedly sleeved on the surface of the rotating disk 5. Outer sealing plates 2 are symmetrically and movably sleeved on the surface of the workbench 1. A control console 7 is fixedly connected to the left side of the top of the workbench 1.

[0033] Please refer to Figure 2 , the rotating mechanism 8 includes a fixed disk 81 and a rotating rod 82. Second bearings 84 and first bearings 83 are symmetrically and fixedly sleeved on the surface of the rotating rod 82. Pin columns 86 are symmetrically fixedly connected to the surface of the first bearings 83. A cylinder 810 is rotatably sleeved on the surface of the second bearing 84 through a rotating assembly 89. The bottom of the rotating rod 82 is fixedly connected to a servo motor 87. A sleeve plate 88 is fixedly sleeved on the surface of the servo motor 87. The top of the rotating rod 82 is fixedly installed on the top of the lower cover 92 through a mounting plate 85. The fixed disk 81 is fixedly connected to the top of the workbench 1. The rotating assembly 89 is fixedly connected to the inner wall of the fixed disk 81. One ends of the pin columns 86 and the sleeve plate 88 are rotatably sleeved on the inner wall of the workbench 1.

[0034] Please refer to Figures 3 - 5 , the clamping mechanism 9 includes an upper cover 91 and a lower cover 92. Screw rods 95 are threadedly sleeved on the surfaces of the upper cover 91 and the lower cover 92. A plurality of placing grooves 93 are symmetrically and fixedly formed on the surfaces of the upper cover 91 and the lower cover 92. A plurality of sliding grooves 910 are symmetrically and fixedly formed on the top of the lower cover 92. A plurality of sliding grooves 97 are symmetrically and fixedly formed on the inner walls of the plurality of sliding grooves 910. A pressing rod 98 is slidably sleeved in the inner cavity of the plurality of sliding grooves 910. Sliding blocks 96 are symmetrically fixedly connected to the surface of the pressing rod 98. A spring 99 is fixedly connected between the surface of the sliding block 96 and the inner wall of the sliding groove 97. One end of the sliding groove 910 communicates with the inner cavity of the placing groove 93. One end of the pressing rod 98 fits with the inner wall of the placing groove 93. The upper cover 91 and the rotating rod 82 are symmetrically fixedly installed through bolts 94. The surface of the sliding block 96 is slidably sleeved on the inner wall of the sliding groove 97. The bottom of the screw rod 95 contacts the other end of the pressing rod 98. The placing groove 93 is aligned with the center line of the injection head 6.

[0035] An embodiment of the present invention provides a technical solution: a method for using a biological cell staining device, specifically including the following steps:

[0036] S1. Clamping: First, place the test tubes containing biological cells in the inner cavity of the placement groove 93 in sequence. Then, rotate the lead screw 95 so that the lead screw 95 moves downward on the surfaces of the upper cover 91 and the lower cover 92, making the bottom of the lead screw 95 abut against the opposite ends of the extrusion rods 98 and extruding the extrusion rods 98. This causes the extrusion rods 98 to move toward one side in the inner cavity of the sliding groove 910, and one end of the extrusion rods 98 extends out to clamp the surfaces of the test tubes in the inner cavity of the placement groove 93 opened on the surface of the lower cover 92. At the same time, the slider 96 will also slide in the inner cavity of the sliding groove 97 as the extrusion rods 98 move, and the spring 99 will be compressed. Additionally, when it is necessary to take out the test tubes, move the lead screw 95 upward so that the lead screw 95 leaves one end of the extrusion rods 98. Under the elastic force of the spring 99, the extrusion rods 98 will be driven to reset, no longer clamping the test tubes, and then the test tubes can be taken out;

[0037] S2. Dye injection: Then, start the hydraulic telescopic rod 3 through the control console 7, causing the hydraulic telescopic rod 3 to rise and fall. Thus, drive the rotating disk 5 to descend through the connecting plate 4, so that the multiple injection heads 6 sleeved on the surface of the rotating disk 5 are inserted into the inner cavity of the clamped test tubes. Then, inject the dye into the test tubes. Then, reset the hydraulic telescopic rod 3 through the control console 7 so that the injection heads 6 extend out of the inner cavity of the test tubes, and then cover the lids on the tops of the test tubes in sequence;

[0038] S3. Rotation: Finally, first start the servo motor 87 on the surface of the sleeve plate 88 through the control console 7, causing the output end of the servo motor 87 to drive the rotating rod 82 to rotate in the inner cavities of the first bearing 83 and the second bearing 84. Then, drive the clamping mechanism 9 to rotate through the mounting disk 85. While rotating, start the cylinder 810 in the inner cavity of the rotating assembly 89 to expand and contract, so that the extending end of the cylinder 810 is rotationally sleeved through the rotation of the rotating assembly 89, pushing the second bearing 84 to tilt to one side. At the same time, under the rotation of the pin 86 and the sleeve plate 88, the rotating rod 82 drives the clamping mechanism 9 to tilt to 15 - 35 °C. Through the tilting and resetting operations of the clamping mechanism 9, the flow direction of the liquid in the test tubes can be changed when the test tubes rotate, which can accelerate the staining of biological cells.

[0039] Furthermore, a through-hole is opened at the top of the workbench 1, and the rotating rod 82 is located in the through-hole. The bottom of the through-hole is fixedly connected with a baffle, and both the sleeve plate 88 and the pin 86 are rotationally sleeved on the baffle.

[0040] Meanwhile, the content not described in detail in this specification belongs to the well-known prior art in the art.

[0041] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.

[0042] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological cell staining device, comprising a workbench (1), characterized in that: In the middle of the top of the workbench (1), a rotating mechanism (8) is provided. On the top of the rotating mechanism (8), a clamping mechanism (9) is provided. On the right side of the top of the workbench (1), a hydraulic telescopic rod (3) is fixedly connected. On the top of the hydraulic telescopic rod (3), a connecting plate (4) is fixedly connected. On the bottom of the connecting plate (4), a rotating disc (5) is fixedly connected. On the surface of the rotating disc (5), a plurality of injection heads (6) are symmetrically and fixedly sleeved. The rotating mechanism (8) includes a fixed disc (81) and a rotating rod (82). On the surface of the rotating rod (82), a second bearing (84) and a first bearing (83) are symmetrically and fixedly sleeved. On the surface of the first bearing (83), pin columns (86) are symmetrically fixedly connected. On the surface of the second bearing (84), a cylinder (810) is rotatably sleeved through a rotating component (89). On the bottom of the rotating rod (82), a servo motor (87) is fixedly connected. On the surface of the servo motor (87), a sleeve plate (88) is fixedly sleeved. The clamping mechanism (9) includes an upper cover (91) and a lower cover (92). On the surfaces of the upper cover (91) and the lower cover (92), screw rods (95) are threadedly sleeved. On the surfaces of the upper cover (91) and the lower cover (92), a plurality of placement grooves (93) are symmetrically and fixedly opened. On the top of the lower cover (92), a plurality of sliding grooves (910) are symmetrically and fixedly opened. On the inner walls of the plurality of sliding grooves (910), sliding grooves (97) are symmetrically fixedly opened. In the inner cavities of the plurality of sliding grooves (910), extrusion rods (98) are slidably sleeved. On the surface of the extrusion rod (98), sliders (96) are symmetrically fixedly connected. Between the surface of the slider (96) and the inner wall of the sliding groove (97), a spring (99) is fixedly connected. The top of the rotating rod (82) is fixedly installed on the top of the lower cover (92) through a mounting disc (85). The fixed disc (81) is fixedly connected to the top of the workbench (1). The rotating component (89) is fixedly connected to the inner wall of the fixed disc (81). One ends of the pin column (86) and the sleeve plate (88) are rotatably sleeved on the inner wall of the workbench (1).

2. The biological cell staining device according to claim 1, wherein: On the surface of the workbench (1), outer sealing plates (2) are symmetrically and movably sleeved. On the left side of the top of the workbench (1), a control console (7) is fixedly connected.

3. The biological cell staining device according to claim 1, characterized in that: One end of the sliding groove (910) communicates with the inner cavity of the placement groove (93). One end of the extrusion rod (98) fits with the inner wall of the placement groove (93).

4. A biological cell staining device according to claim 1, characterized in that: Between the upper cover (91) and the lower cover (92), they are symmetrically fixedly installed through bolts (94). The surface of the slider (96) is slidably sleeved on the inner wall of the sliding groove (97).

5. A biological cell staining device according to claim 1, characterized in that: The bottom of the screw rod (95) contacts the other end of the extrusion rod (98). The placement groove (93) is aligned with the center line of the injection head (6).

6. A method for using the biological cell staining device according to any one of claims 1-5, characterized in that: Specifically, it includes the following steps: S1. Clamping: First, place the test tubes containing biological cells in the inner cavity of the placement groove (93) in sequence. Then, rotate the lead screw (95) so that the lead screw (95) moves downward on the surfaces of the upper cover (91) and the lower cover (92), making the bottom of the lead screw (95) abut against the opposite end of the extrusion rod (98) and extruding the extrusion rod (98). This causes the extrusion rod (98) to move toward one side in the inner cavity of the sliding groove (910), with one end of the extrusion rod (98) protruding to clamp the surface of the test tube in the inner cavity of the placement groove (93) opened on the surface of the lower cover (92). At the same time, the slider (96) will also slide in the inner cavity of the sliding groove (97) as the extrusion rod (98) moves, and the spring (99) will be compressed. Additionally, when it is necessary to take out the test tube, move the lead screw (95) upward so that the lead screw (95) leaves one end of the extrusion rod (98). Under the elastic force of the spring (99), the extrusion rod (98) will be driven to reset, and it will no longer clamp the test tube, allowing the test tube to be taken out; S2. Stain injection: Then, start the hydraulic telescopic rod (3) through the console (7) to make the hydraulic telescopic rod (3) rise and fall. Thus, drive the rotating disk (5) to descend through the connecting plate (4), so that multiple injection heads (6) sleeved on the surface of the rotating disk (5) are inserted into the inner cavity of the clamped test tube. Then, inject the stain into the test tube. Then, reset the hydraulic telescopic rod (3) through the console (7) to make the injection heads (6) protrude from the inner cavity of the test tube. Then, cover the lids on the top of the test tubes in sequence; S3. Rotation: Finally, first start the servo motor (87) on the surface of the sleeve plate (88) through the console (7), so that the output end of the servo motor (87) drives the rotating rod (82) to rotate in the inner cavities of the first bearing (83) and the second bearing (84). Then, drive the clamping mechanism (9) to rotate through the mounting plate (85). While rotating, start the cylinder (810) in the inner cavity of the rotating assembly (89) to expand and contract, so that the protruding end of the cylinder (810) is rotationally sleeved through the rotation of the rotating assembly (89) to push the second bearing (84) to tilt to one side. At the same time, under the rotation of the pin column (86) and the sleeve plate (88), the rotating rod (82) drives the clamping mechanism (9) to tilt to 15 - 35 °C. Through the tilting and resetting operations of the clamping mechanism (9), the flow direction of the liquid in the test tube can be changed when the test tube rotates, which can accelerate the staining of biological cells.

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