A tissue sectioning knife and method of making the same

Abstract

The present application belongs to the technical field of medical equipment manufacturing, and particularly relates to a tissue sectioning knife and a manufacturing method thereof. The tissue sectioning knife provided by the present application is provided with a first front peak area, a second front peak area, a first middle peak area, a second middle peak area, a first rear peak area and a second rear peak area, and the first, third and fifth blade angles decrease in sequence; the second, fourth and sixth blade angles decrease in sequence; the first blade angle is smaller than the second blade angle, and the third blade angle is smaller than the fourth blade angle; the fifth blade angle is larger than the sixth blade angle; the blade edge is asymmetric dagger-shaped, effectively avoiding the sticking knife defect during tissue sectioning, and the sharpness is better, and the sectioning effect is better; the obtained tissue section is more stable in shape, and meanwhile, the top end surface of the tip of the blade edge of the tissue sectioning knife provided by the present application is an inclined plane, and is not easy to curl, and is more durable, and the tissue sectioning knife prepared by the present application can be reused for 30-35 times.

Description

Technical Field

[0001] The invention belongs to the technical field of medical equipment manufacturing, and particularly relates to a tissue slicer and a manufacturing method thereof. Background Art

[0002] Pathological tissue examination is a commonly used method for clinical diagnosis of diseases, and its scope is relatively wide. Pathology laboratory tissue sectioning refers to the process of taking a certain size of diseased tissue and making a pathological section using histopathological methods. The diseased tissue is usually embedded in a paraffin block, cut into thin slices using a microtome, and then stained with hematoxylin and eosin (HE), and the lesion is further examined under a microscope. Therefore, tissue sectioning plays an extremely important role in pathology experiments. The entire pathology experiment process is based on the successful preparation of tissue sections, so that the entire pathology can be better studied, which can also effectively ensure the smooth progress of the entire pathology experiment.

[0003] Tissue slicers are a key factor in determining the quality of pathology tissue sections. Due to the diverse morphology and hardness of tissues, the performance requirements for these slicers are extremely high. Until now, domestically produced blades have struggled to meet the requirements of hospitals and research institutions, requiring them to be imported. The market for disposable pathology tissue blades is dominated by foreign manufacturers. Existing disposable tissue blades typically consist of a blade body, blade edge, and blade tip. However, these blades are prone to sticking and curling, resulting in a short service life. Summary of the Invention

[0004] In view of this, the present invention provides a tissue slicer and a manufacturing method thereof. The tissue slicer provided by the present invention is not prone to sticking to the blade, does not curl, and has a long service life.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The present invention provides a tissue slicer, comprising a blade body, a blade edge and a blade head. Along the length direction of the tissue slicer (201), the blade edge 206 is provided with a front area A, a middle peak area B and a back front area C. Along the central axis of the thickness of the tissue slicer 201, the front area A is provided with a first front area A1 and a second front area A2 located on both sides of the central axis. A first blade angle formed by an edge of the first front area A1 and the central axis is smaller than a second blade angle formed by an edge of the second front area A2 and the central axis.

[0007] Along the central axis of the thickness of the tissue slicer 201, the central peak area B is provided with a first central peak area B1 and a second central peak area B2 located on both sides of the central axis, and a third blade angle formed by an edge of the first central peak area B1 and the central axis is smaller than a fourth blade angle formed by an edge of the second central peak area B2 and the central axis;

[0008] Along the central axis of the thickness of the tissue slicer 201, the back edge area C is provided with a first back edge area C1 and a second back edge area C2 located on both sides of the central axis, and the fifth blade angle formed by the edge of the first back edge area C1 and the central axis is greater than the sixth blade angle formed by the edge of the second back edge area C2 and the central axis;

[0009] The first blade angle, the third blade angle and the fifth blade angle decrease in sequence; the second blade angle, the fourth blade angle and the sixth blade angle decrease in sequence;

[0010] The top end surface of the tip of the blade 206 is an inclined plane, and the angle between the first projection line of the inclined plane in the plane formed by the thickness direction and the length direction and the thickness direction is 5-10°.

[0011] Preferably, the thickness of the tip is 0.01±0.005 mm.

[0012] Preferably, the intersection of the second projection line and the first projection line in the plane formed by the edge of the second front area A2 in the thickness direction and the length direction is taken as the vertex, and the angle between the first projection line and the thickness direction is 5-10°.

[0013] Preferably, the first vertex of the first cutting edge angle on the central axis is located at the same position as the second vertex of the second cutting edge angle on the central axis;

[0014] Taking the first vertex as the starting position and the point where the edge end of the first front area A1 is projected onto the central axis as the ending position, the length between the starting position and the ending position is the vertical length of the first front area A1; the length where the edge of the first middle peak area B1 is projected onto the central axis is the vertical length of the first middle peak area B1; the length where the edge of the first rear front area C1 is projected onto the central axis is the vertical length of the first rear front area C1;

[0015] Taking the second vertex as the starting position and the point where the edge end of the second front area A2 is projected onto the central axis as the ending position, the length between the starting position and the ending position is the vertical length of the second front area A2; the length where the edge of the second middle peak area B2 is projected onto the central axis is the vertical length of the second middle peak area B2; the length where the edge of the second rear front area C2 is projected onto the central axis is the vertical length of the second rear front area C2;

[0016] The vertical length of the second front area A2, the vertical length of the first front area A1, the vertical length of the second center peak area B2, the vertical length of the first center peak area B1, the vertical length of the first rear area C1 and the vertical length of the second rear area C2 increase successively.

[0017] The present invention provides a method for manufacturing the tissue slicer described in the above technical solution, comprising the following steps:

[0018] The raw materials of the tissue slicer are sequentially loaded and pulled to obtain a discharge belt;

[0019] The discharge belt is punched and formed to obtain a slicing knife belt having a knife body and a blade structure;

[0020] The slicing knife band is sequentially subjected to a gradient calcination treatment, a freezing treatment, and a low-temperature tempering treatment to obtain a heat-treated knife band, wherein the gradient calcination treatment and the low-temperature tempering treatment are both performed in a protective gas;

[0021] Grinding the blade of the heat-treated knife band to obtain a tissue slice knife prototype;

[0022] The tissue slicer prototype is sequentially cleaned, coated, sintered and cut to obtain the tissue slicer.

[0023] Preferably, the stamping pressure is 25 to 45 tons.

[0024] Preferably, the gradient calcination treatment includes a first calcination treatment, a second calcination treatment, a third calcination treatment and a fourth calcination treatment in sequence; the holding temperature of the first calcination treatment is 900-1000°C, and the holding time of the first calcination treatment is 1-2 minutes; the holding temperature of the second calcination treatment is 1050-1150°C, and the holding time of the second calcination treatment is 2-3 minutes; the holding temperature of the third calcination treatment is 1200-1250°C, and the holding time of the third calcination treatment is 1-3 minutes; the holding temperature of the fourth calcination treatment is 1300-1350°C, and the holding time of the fourth calcination treatment is 1-2 minutes.

[0025] Preferably, the holding temperature of the freezing treatment is -80 to -100°C, and the holding time of the freezing treatment is 5 to 10 minutes;

[0026] The holding temperature of the low-temperature tempering treatment is 290-310° C., and the holding time of the low-temperature tempering treatment is 8-15 minutes.

[0027] Preferably, the grinding adopts an 8-stage spiral sharpening machine; the grinding power is 3-4 kW; the grinding includes coarse grinding of the rear edge area C, fine grinding of the middle edge area B and fine grinding of the front edge area A.

[0028] Preferably, the freezing treatment obtains a frozen knife belt, and before the low-temperature tempering treatment, the frozen knife belt is subjected to an alcohol spray treatment to obtain a spray knife belt, and the spray knife belt is subjected to the low-temperature tempering treatment; the time of the alcohol spray treatment is 0.5 to 1 minute.

[0029] The application provides a tissue sectioning knife, a blade 206 of the tissue sectioning knife 201 is provided with a first front peak area A1, a second front peak area A2, a first middle peak area B1, a second middle peak area B2, a first rear peak area C1 and a second rear peak area C2, and the first blade angle, the third blade angle and the fifth blade angle are sequentially reduced; the second blade angle, the fourth blade angle and the sixth blade angle are sequentially reduced; the first blade angle is smaller than the second blade angle, the third blade angle is smaller than the fourth blade angle; the fifth blade angle is larger than the sixth blade angle; so that the blade 206 of the tissue sectioning knife 201 forms an asymmetric dagger shape composed of six different angles and six edge slopes, effectively avoiding the sticking knife defect during tissue sectioning, better sharpness and better sectioning effect; so that the shape of the tissue sectioning is more stable, and the top surface of the tip of the blade 206 of the tissue sectioning knife 201 is an inclined plane, a first projection line of the inclined plane in the plane formed in the thickness direction and the length direction and the thickness direction forms an angle of 5-10°, compared with no inclined angle, the blade of the tissue sectioning knife is asymmetrically shaped and the tip of the blade has an inclined end surface with an included angle, so that the blade is not easy to roll, and is more durable; the tissue sectioning knife prepared by the application can obtain experimental sections with moderate thickness and smooth incisions even if the bone tissue is frozen sectioned; compared with the current disposable sectioning knife, the tissue sectioning knife prepared by the application can be reused for 30-35 times.

[0030] The present invention provides a method for manufacturing the tissue slicer 201 described in the above technical solution, comprising the following steps: sequentially loading and pulling the raw materials of the tissue slicer 201 to obtain a discharge belt; stamping the discharge belt to obtain a slicer belt, wherein the slicer belt has a blade body and a blade structure; sequentially subjecting the slicer belt to a gradient calcination treatment, a freezing treatment, and a low-temperature tempering treatment to obtain a heat-treated blade belt, wherein the gradient calcination treatment and the low-temperature tempering treatment are both performed in a protective gas; grinding the blade of the heat-treated blade belt to obtain a tissue slicer prototype; sequentially cleaning, coating, sintering, and cutting the tissue slicer prototype to obtain the tissue slicer 201. The manufacturing method provided by the present invention produces a high-hardness slicing blade band through stamping, which can effectively improve the sharpness of tissue slices. Then, a gradient calcination treatment can transform the microstructure crystal form of the slicing blade band, thereby improving the hardness of the slicing blade band. Subsequently, a freezing treatment is adopted. Compared with traditional cooling water cooling, the freezing treatment adopted by the present invention can achieve a raw and cold treatment of the slicing blade band material, effectively improving the hardness and toughness of the material. Then, a low-temperature tempering treatment can make the microstructure of the material more uniform, further improving the hardness and toughness of the material. Finally, through grinding, cleaning, coating, sintering, and cutting, the above-mentioned structure and high hardness and toughness of the tissue slicing blade 201 are obtained. The manufacturing method provided by the present invention has a high degree of automation, high processing efficiency, and saves labor costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 Schematic diagram of the manufacturing method and equipment provided by the embodiment of the present invention;

[0032] Among them, 1-first automatic unloading rack, 2-high static and high speed stamping equipment, 3-second automatic unloading machine, 4-ladder heat treatment tunnel furnace, 5-freezing equipment, 6-low temperature tempering equipment, 7-grinding equipment, 8-cleaning equipment, 9-coating and sintering equipment, 10-cutting equipment, 11-inspection and packaging equipment;

[0033] Figure 2 Schematic diagram of a tissue slicer provided in an embodiment of the present invention;

[0034] Among them, 201 is a tissue slicer, 202 is a blade, 203 is a blade, 204 is a process opening, 205 is a first process hole, 206 is a blade edge, and 207 is a second process hole;

[0035] Figure 3 An enlarged cross-section of the blade of a tissue slicer provided by an embodiment of the present invention and a schematic diagram of the angles of the blade;

[0036] Figure 4 An enlarged view of the front region A of the tissue slicer blade provided by an embodiment of the present invention;

[0037] Figure 5 The tissue slicer provided in Example 1 of the present invention is used for slicing tissue slices. DETAILED DESCRIPTION

[0038] The present invention provides a tissue slicer, comprising a blade body, a blade edge, and a blade head. Along the length of the tissue slicer 201, the blade edge 206 is provided with a front region A, a middle peak region B, and a rear front region C. Along the central axis of the thickness of the tissue slicer 201, the front region A is provided with a first front region A1 and a second front region A2 located on either side of the central axis. A first blade angle formed between the edge of the first front region A1 and the central axis is smaller than a second blade angle formed between the edge of the second front region A2 and the central axis.

[0039] Along the central axis of the thickness of the tissue slicer 201, the central peak area B is provided with a first central peak area B1 and a second central peak area B2 located on both sides of the central axis, and a third blade angle formed by an edge of the first central peak area B1 and the central axis is smaller than a fourth blade angle formed by an edge of the second central peak area B2 and the central axis;

[0040] Along the central axis of the thickness of the tissue slicer 201, the back edge area C is provided with a first back edge area C1 and a second back edge area C2 located on both sides of the central axis, and the fifth blade angle formed by the edge of the first back edge area C1 and the central axis is greater than the sixth blade angle formed by the edge of the second back edge area C2 and the central axis;

[0041] The first blade angle, the third blade angle and the fifth blade angle decrease in sequence; the second blade angle, the fourth blade angle and the sixth blade angle decrease in sequence;

[0042] The top end surface of the tip of the blade 206 is an inclined plane, and the angle between the first projection line of the inclined plane in the plane formed by the thickness direction and the length direction and the thickness direction is 5-10°.

[0043] The tissue slicing knife 201 provided by the present invention includes a blade body 202 along the width direction of the tissue slicing knife 201 .

[0044] As a specific embodiment of the present invention, the surface of the blade body 202 has a first process hole 205 and a second process hole 207 .

[0045] In the present invention, the first process hole 205 and the second process hole 207 are used to fix the blade body in the grinding device to facilitate the grinding of the blade edge.

[0046] As a specific embodiment of the present invention, both the first process hole 205 and the second process hole 207 are through holes.

[0047] As a specific embodiment of the present invention, the back of the blade body 202 has a process opening 204 .

[0048] In the present invention, the process opening 204 cooperates with the first process hole 205 and the second process hole 207 to fix the blade body in the grinding device, so as to facilitate the grinding of the blade edge.

[0049] As a specific embodiment of the present invention, the blade body 202 has a length of 80±0.15 mm, a width of 8±0.1 mm, and a thickness of 0.26±0.01 mm.

[0050] The tissue slicing knife 201 provided by the present invention includes a blade 203 fixedly connected to the blade body 202 along the width direction of the tissue slicing knife 201 .

[0051] As a specific embodiment of the present invention, the blade body 202 and the blade 203 are integrally formed.

[0052] As a specific embodiment of the present invention, the length of the blade 203 is 80±0.15 mm.

[0053] The present invention has no special requirements on the width and thickness of the blade 203 .

[0054] The tissue slicing knife 201 provided by the present invention includes a blade 206 fixedly connected to the blade 203 along the width direction of the tissue slicing knife 201 .

[0055] As a specific embodiment of the present invention, the blade 206 is formed by grinding the blade 203 .

[0056] As a specific embodiment of the present invention, the front area A, the middle area B and the rear area C are integrally formed.

[0057] As a specific embodiment of the present invention, the first forward area A1 and the second forward area A2 are integrally formed.

[0058] As a specific embodiment of the present invention, the first mid-peak area B1 and the second mid-peak area B2 are integrally formed.

[0059] As a specific embodiment of the present invention, the first rear front area C1 and the second rear front area C2 are integrally formed.

[0060] As a specific embodiment of the present application, the first cutting edge angle θ(A1) is 16.9±0.5°, the second cutting edge angle θ(A2) is 19.4±0.5°, the third cutting edge angle θ(B1) is 9.4±0.5°, the fourth cutting edge angle θ(B2) is 10.8±0.5°, the fifth cutting edge angle θ(C1) is 6.7±0.5°, and the sixth cutting edge angle θ(C2) is 6.4±0.5°.

[0061] As a specific embodiment of the present application, the first cutting edge angle is located at the same position as the second cutting edge angle on the center axis; the length between the starting position of the first vertex and the ending position of the edge end of the first leading edge A1 projected onto the center axis is the vertical length of the first leading edge A1; the length of the edge of the first middle peak B1 projected onto the center axis is the vertical length of the first middle peak B1; the length of the edge of the first trailing edge C1 projected onto the center axis is the vertical length of the first trailing edge C1; the length between the starting position of the second vertex and the ending position of the edge end of the second leading edge A2 projected onto the center axis is the vertical length of the second leading edge A2; the length of the edge of the second middle peak B2 projected onto the center axis is the vertical length of the second middle peak B2; the length of the edge of the second trailing edge C2 projected onto the center axis is the vertical length of the second trailing edge C2; the vertical length of the second leading edge A2, the vertical length of the first leading edge A1, the vertical length of the second middle peak B2, the vertical length of the first middle peak B1, the vertical length of the first trailing edge C1, and the vertical length of the second trailing edge C2 increase in turn.

[0062] As a specific embodiment of the present application, the vertical length L(A1) of the first leading edge A1 is 0.057±0.005mm; the vertical length L(A2) of the second leading edge A2 is 0.051±0.005mm; the vertical length L(B1) of the first middle peak B1 is 0.178±0.005mm; the vertical length L(B2) of the second middle peak B2 is 0.085±0.005mm; the vertical length L(C1) of the first trailing edge C1 is 0.731±0.005mm; and the vertical length L(C2) of the second trailing edge C2 is 0.799±0.005mm.

[0063] As a specific embodiment of the present application, the thickness of the tip is 0.01±0.005mm.

[0064] In a specific embodiment of the present invention, the intersection of the second projection line and the first projection line in the plane formed by the edge of the second front area A2 in the thickness direction and the length direction is taken as the vertex, and the angle between the first projection line and the thickness direction is 5 to 10 degrees.

[0065] In the present invention, the angle between the first projection line and the thickness direction is preferably 5.5 to 9 degrees.

[0066] As a specific embodiment of the present invention, the angle between the first projection line and the thickness direction is 5°.

[0067] As a specific embodiment of the present invention, the angle between the first projection line and the thickness direction is 5.4°.

[0068] As a specific embodiment of the present invention, the angle between the first projection line and the thickness direction is 7°.

[0069] As a specific embodiment of the present invention, the angle between the first projection line and the thickness direction is 8°.

[0070] As a specific embodiment of the present invention, the angle between the first projection line and the thickness direction is 10°.

[0071] As a specific embodiment of the present invention, the length of the blade peak 206 is 80±0.15 mm.

[0072] The present invention provides a method for manufacturing the tissue slicer 201 described in the above technical solution, comprising the following steps:

[0073] The raw materials of the tissue slicer 201 are sequentially loaded and pulled to obtain a discharge belt;

[0074] The discharge belt is punched and formed to obtain a slicing knife belt having a knife body and a blade structure;

[0075] The slicing knife band is sequentially subjected to a gradient calcination treatment, a freezing treatment, and a low-temperature tempering treatment to obtain a heat-treated knife band, wherein the gradient calcination treatment and the low-temperature tempering treatment are both performed in a protective gas;

[0076] Grinding the blade of the heat-treated knife belt to obtain a tissue slice knife prototype;

[0077] The tissue slicer prototype is cleaned, coated, sintered and cut to obtain the tissue slicer 201.

[0078] In the present invention, the raw materials of the tissue slicing knife 201 are sequentially loaded and pulled to obtain a discharge belt.

[0079] In the present invention, the raw material is preferably SUS420 stainless steel or SUS440 stainless steel.

[0080] The present invention has no special requirements on the specific implementation process of the loading and pulling.

[0081] In the present invention, the pulling speed is preferably 20 to 30 s / m.

[0082] After obtaining the discharge belt, the present invention punches the discharge belt into shape to obtain a slicing knife belt having a knife body and a blade structure.

[0083] In the present invention, the stamping is preferably performed in a high-speed precision punch press.

[0084] In the present invention, the stamping pressure is preferably 25 to 45 tons, more preferably 28 to 40 tons.

[0085] In the present invention, slicing knife prototypes are arranged on the slicing knife belt, and each slicing knife prototype has a blade body and a blade structure, and the blades are located on the same side of the slicing knife belt.

[0086] In the present invention, the surface of the blade body is preferably stamped to obtain the first process hole and the second process hole.

[0087] In the present invention, the back of the blade is preferably formed by stamping to obtain a process opening.

[0088] In the present invention, when the raw material is preferably SUS420 stainless steel, the hardness (HRC) of the slicing knife belt after stamping is preferably 57.5±1~58.5±1, which will not cause adverse effects on the subsequent processing of the slicing knife belt, but can improve the sharpness of the blade.

[0089] After obtaining the slicing knife belt, the present invention sequentially performs gradient calcination treatment, freezing treatment and low-temperature tempering treatment on the slicing knife belt to obtain a heat-treated knife belt, wherein the gradient calcination treatment and the low-temperature tempering treatment are both performed in a protective gas.

[0090] In the present invention, the gradient calcination treatment preferably includes sequentially performing a first calcination treatment, a second calcination treatment, a third calcination treatment and a fourth calcination treatment; the holding temperature of the first calcination treatment is preferably 900-1000°C, more preferably 915-995°C; the holding time of the first calcination treatment is preferably 1-2 min, more preferably 1.2-1.9 min; the holding temperature of the second calcination treatment is preferably 1050-1150°C, more preferably 1055-1145°C; the holding time of the second calcination treatment is preferably The holding time of the third calcination treatment is preferably 1-3 min, more preferably 1.2-2.5 min; the holding temperature of the fourth calcination treatment is preferably 1300-1350 ° C, more preferably 1310-1345 ° C; the holding time of the fourth calcination treatment is preferably 1-2 min, more preferably 1.1-1.9 min.

[0091] In the present invention, the gradient calcination treatment is carried out in a protective gas, and the protective gas is preferably nitrogen, and the purity of the nitrogen is preferably 99.99%.

[0092] In the present invention, the gradient calcination treatment is preferably carried out in a ladder-type heat treatment tunnel furnace.

[0093] In the present invention, when the gradient calcination treatment is carried out in the ladder-type heat treatment tunnel furnace, the temperature of each zone in the gradient calcination process is controlled by a temperature control table and an automatic system, and the temperature stability of each zone can be ensured during batch production.

[0094] In the present invention, the holding temperature of the freezing treatment is preferably -80 to -100°C, more preferably -85 to -95°C; the holding time of the freezing treatment is preferably 5 to 10 minutes, more preferably 5.5 to 8 minutes.

[0095] In the present invention, the freezing treatment obtains a frozen knife belt. Before the low-temperature tempering treatment, the present invention preferably further includes subjecting the frozen knife belt to an alcohol spray treatment to obtain a sprayed knife belt, and subjecting the sprayed knife belt to the low-temperature tempering treatment; the time of the alcohol spray treatment is preferably 0.5 to 1 min, more preferably 0.55 to 0.95 min.

[0096] The present invention preferably uses the alcohol spraying treatment to maintain the low temperature of the freezing knife belt and prevent the freezing knife belt from changing color when in contact with air, thereby affecting the final quality.

[0097] In the present application, the holding temperature of the low-temperature tempering treatment is preferably 290-310 DEG C, and more preferably 295-305 DEG C; the holding time of the low-temperature tempering treatment is preferably 8-15 min, and more preferably 8.5-13 min.

[0098] In the present application, the low-temperature tempering treatment is carried out in a protective gas, which is preferably nitrogen, and the purity of the nitrogen is preferably 99.99%.

[0099] After obtaining the heat-treated knife strip, the present application grinds the cutting edge of the heat-treated knife strip to obtain a histological section knife blank.

[0100] In the present application, the grinding power is preferably 3-4 kW, and more preferably 3.2-3.8 kW.

[0101] In the present application, the grinding preferably includes coarse grinding of the front area C, fine grinding of the middle area B, and precision grinding of the front area A.

[0102] In the present application, the grinding preferably uses an 8-section spiral grinding machine. Compared with the conventional grinding machine, the present application preferably uses the 8-section spiral grinding machine to ensure the stability and consistency of each angle of the heat-treated knife strip, and to ensure that each angle is controlled within the standard range.

[0103] In the present application, the grinding is preferably carried out under the observation condition of a microscope, and the magnification of the microscope is preferably 80 times.

[0104] The present application preferably detects the grinding condition of the grinding machine and the heat-treated knife strip on line through the microscope. When the grinding wheel is worn and the heat-treated knife strip is abnormal, the grinding equipment realizes automatic compensation or automatic shutdown, thereby ensuring the stability and consistency of the product production process.

[0105] After obtaining the histological section knife blank, the present application sequentially carries out cleaning, film plating, sintering forming, and cutting on the histological section knife blank to obtain the histological section knife.

[0106] In the present application, the cleaning is preferably carried out under the condition of ultrasonic.

[0107] In the present application, when the cleaning is carried out under the condition of ultrasonic, the power of the ultrasonic is preferably 20-40 kW, and the time of the ultrasonic is preferably 5-10 min.

[0108] The present application preferably removes the grinding chips through the cleaning.

[0109] In the present application, the film plating preferably includes sequentially carrying out first film plating and second film plating.

[0110] In the present application, the first film plating preferably obtains a chromium film.

[0111] In the present invention, the first coating is preferably performed on the blade edge and the blade front.

[0112] In the present invention, the first coating is preferably implemented by plasma spraying.

[0113] In the present invention, the first coating is preferably performed under vacuum conditions or negative pressure conditions.

[0114] In the present invention, the negative pressure condition is preferably 15 to 20 KPa.

[0115] In the present invention, the holding temperature of the first coating is preferably 200-350°C, more preferably 220-330°C.

[0116] In the present invention, the holding time of the first coating is preferably 3 to 10 minutes, more preferably 3.5 to 8 minutes.

[0117] In the present invention, the second coating film is preferably a Teflon film.

[0118] In the present invention, the second coating is preferably performed by spraying, more preferably by atomized spraying.

[0119] In the present invention, the second coating time is preferably 3 to 10 minutes, more preferably 3.5 to 8 minutes.

[0120] In the present invention, during the second coating, the present invention preferably ensures that 100% of the blade edge is uniformly wrapped by the Teflon film.

[0121] In the present invention, the wrapping thickness of the Teflon film at the blade edge is preferably 0.001 to 0.003 mm, more preferably 0.0015 to 0.0025 mm.

[0122] In the present invention, the second coating is preferably performed in a closed environment.

[0123] In the present invention, the holding temperature of the sintering molding is preferably 500-650°C, more preferably 550-600°C.

[0124] In the present invention, the holding time for sintering into a shape is preferably 8 to 15 minutes, more preferably 9 to 12 minutes.

[0125] In the present invention, the tissue slicer 201 is preferably obtained by cutting.

[0126] In the present invention, the cutting obtains a primary tissue slice knife product. In the present invention, the primary tissue slice knife product is preferably post-processed to obtain the tissue slice knife.

[0127] In the present application, the post-processing preferably comprises detection and sterilization packaging in sequence.

[0128] In the present application, the detection is preferably non-destructive detection.

[0129] In the present application, the sterilization packaging is preferably performed in a dust-free workshop.

[0130] The technical solutions in the present application will be described clearly and completely in combination with the embodiments in the present application. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative work are within the protection scope of the present application.

[0131] Embodiment 1

[0132] According to the manufacturing method flow and the device schematic diagram shown in the figure, the tissue sectioning knife is manufactured: Figure 1

[0133] The blade raw material is placed on the rack, and the material belt is pulled out, which is made of SUS420 stainless steel.

[0134] The material belt is punched by a 30-ton high-speed precision punch to obtain a sectioning knife belt, and the sectioning knives are arranged on the sectioning knife belt, and the cutting edges of each sectioning knife are arranged on the same side of the sectioning knife belt.

[0135] The sectioning knife belt is sequentially subjected to gradient calcination treatment, freezing treatment, alcohol spraying treatment and low-temperature tempering treatment, the gradient heat treatment is divided into four stages, the first stage temperature is 1000℃, the second stage temperature is 1100℃, the third stage temperature is 1200℃, and the fourth stage temperature is preferably 1300℃, the first stage holding time is 1min, the second stage holding time is 3min, the third stage holding time is 1min, and the fourth stage holding time is 1min; the freezing treatment temperature is-80℃, and the time is 10min; the alcohol spraying time is 0.5min; the low-temperature tempering treatment temperature is 300℃, and the time is preferably 10min; the gradient heat treatment and the low-temperature tempering treatment are both performed under the protection of 99.99% high-purity nitrogen.

[0136] ​The blade of the heat-treated slicing knife belt is ground using a knife grinder; the knife grinder is an 8-stage spiral knife grinder with a power of 3kW: the grinding is divided into coarse grinding, fine grinding and fine grinding, among which coarse grinding mainly grinds the rear front surface C, fine grinding mainly grinds the center front surface B, and fine grinding mainly grinds the front surface A. The blade angles of the front surface A, the center surface B and the back surface C are: θ(A1)=16.9°, θ(A2)=19.4°, θ(B1)=9.4°, θ(B2)=10.8°, θ(C1)=6.7° and θ(C2)=6.4° respectively; the vertical lengths of the front surface A, the center surface B and the back surface C are: L(A1)=0.057mm, L(A2)=0.051mm, L(B1)=0.178mm, L(B2)=0.085mm, L(C1)=0.731mm and L(C2)=0.799mm respectively; the length of the edge oblique line of the first front area is smaller than the length of the edge oblique line of the second front area, the tip surface of the blade is an inclined plane, the angle between the inclined plane and the thickness direction is 7°, and the thickness of the blade tip is 0.01mm.

[0137] Cleaning: The slicer (201) is ultrasonically cleaned, the power of the ultrasonic cleaning machine is 20 kW, and the time is 10 minutes.

[0138] Coating and sintering: The coating is divided into two layers. The first layer is to plasma thermally spray metal chromium on the blade and edge of the slicing knife 201 under vacuum or low pressure conditions. The second layer is to evenly spray Teflon on the cutting edge in a closed environment, and finally heated and sintered to form. The air partial pressure of the vacuum or low pressure conditions in the first layer of chromium plating is 15KPa, the temperature is 300℃, and the time is 5 minutes. The second layer is sprayed with Teflon, and the spraying method is atomized spraying for 5 minutes to ensure that the blade edge is 100% evenly covered with Teflon, with a wrapping thickness of 0.001mm. The temperature of the sintering is 500℃ and the time is 15 minutes.

[0139] Cutting: cutting the slicing blade belt 200 into separate slicing blades 201;

[0140] Inspection and sterilization packaging: The cut blades are subjected to non-destructive inspection and then sterilized and packaged in a dust-free workshop.

[0141] The tissue slicer obtained in this embodiment has a length of 80 mm, a width of 8 mm, and a thickness of 0.25 mm.

[0142] Example 2

[0143] according to Figure 1 The manufacturing method process and equipment schematic diagram shown are for manufacturing tissue slice knives:

[0144] Place the blade raw material on the material rack and pull out the material belt, which is made of SUS440 stainless steel.

[0145] A slicing knife belt is obtained by punching a material strip with a 25-ton high-speed precision punch press. Slicing knives are arranged on the slicing knife belt, and the blades of each slicing knife are arranged on the same side of the slicing knife belt.

[0146] The slicing knife strip is subjected to gradient calcination treatment-freezing treatment-alcohol spraying treatment-low-temperature tempering treatment in sequence. The gradient heat treatment is divided into 4 sections, the temperature of the first section is 950°C, the temperature of the second section is 1050°C, the temperature of the third section is 1250°C, and the temperature of the fourth section is preferably 1350°C. The insulation time of the first section is 2 minutes, the insulation time of the second section is 2 minutes, the insulation time of the third section is 3 minutes, and the insulation time of the fourth section is 1 minute; the temperature of the freezing treatment is -85°C, and the time is 9 minutes; the alcohol spraying time is 1 minute; the temperature of the low-temperature tempering treatment is 290°C, and the time is preferably 12 minutes; the gradient heat treatment and the low-temperature tempering treatment are both carried out under the protection of 99.99% high-purity nitrogen.

[0147] The blade of the heat-treated slicing knife belt is ground using a knife grinder; the knife grinder is an 8-stage spiral knife grinder with a power of 3.2kW: grinding is divided into coarse grinding, fine grinding and fine grinding, among which coarse grinding mainly grinds the rear front surface C, fine grinding mainly grinds the center front surface B, and fine grinding mainly grinds the front surface A. The blade angles of the front surface A, the center surface B and the back surface C are: θ(A1)=16.8°, θ(A2)=19.5°, θ(B1)=9.5°, θ(B2)=10.9°, θ(C1)=6.6° and θ(C2)=6.3° respectively; the vertical lengths of the front surface A, the center surface B and the back surface C are: L(A1)=0.059mm, L(A2)=0.052mm, L(B1)=0.174mm, L(B2)=0.087mm, L(C1)=0.734mm and L(C2)=0.797mm respectively; the length of the edge oblique line of the first front area is smaller than the length of the edge oblique line of the second front area, the tip surface of the blade is an inclined plane, the angle between the inclined plane and the thickness direction is 7°, and the thickness of the blade tip is 0.01mm.

[0148] Cleaning: The slicer 201 is ultrasonically cleaned with an ultrasonic cleaning machine having a power of 25 kW and a cleaning time of 8 minutes.

[0149] Coating and sintering: The coating is divided into two layers. The first layer is to plasma thermally spray metal chromium on the blade and edge of the slicing knife 201 under vacuum or low pressure conditions. The second layer is to evenly spray Teflon on the cutting edge in a closed environment, and finally heat and sinter to form. The air partial pressure of the vacuum or low pressure conditions in the first layer of chromium plating is 16KPa, the temperature is 250℃, and the time is 7 minutes. The second layer is sprayed with Teflon, and the spraying method is atomized spraying for 8 minutes to ensure that the blade edge is 100% evenly covered with Teflon, with a wrapping thickness of 0.002mm. The temperature of the sintering is 550℃ and the time is 9 minutes.

[0150] Cutting: cutting the slicing blade belt 200 into separate slicing blades 201;

[0151] Inspection and sterilization packaging: The cut blades are subjected to non-destructive inspection and then sterilized and packaged in a dust-free workshop.

[0152] The tissue slicer obtained in this embodiment has a length of 80.03 mm, a width of 8.05 mm, and a thickness of 0.252 mm.

[0153] Example 3

[0154] according to Figure 1 The manufacturing method process and equipment schematic diagram shown are for manufacturing tissue slice knives:

[0155] Place the blade raw material on the material rack and pull out the material belt, which is made of SUS420 stainless steel.

[0156] A material strip is punched with a 40-ton high-speed precision punch press to obtain a slicing knife strip, on which slicing knives are arranged, and the blades of each slicing knife are arranged on the same side of the slicing knife strip.

[0157] The slicing knife strip is subjected to gradient calcination treatment-freezing treatment-alcohol spraying treatment-low-temperature tempering treatment in sequence. The gradient heat treatment is divided into 4 sections, the temperature of the first section is 950°C, the temperature of the second section is 1150°C, the temperature of the third section is 1250°C, and the temperature of the fourth section is preferably 1350°C. The insulation time of the first section is 2 minutes, the insulation time of the second section is 2 minutes, the insulation time of the third section is 1 minute, and the insulation time of the fourth section is 2 minutes; the temperature of the freezing treatment is -90°C and the time is 7 minutes; the alcohol spraying time is 0.6 minutes; the temperature of the low-temperature tempering treatment is 300°C and the time is preferably 10 minutes; the gradient heat treatment and the low-temperature tempering treatment are both carried out under the protection of 99.99% high-purity nitrogen.

[0158] The cutting edge of the heat-treated sectioning blade is ground by a grinding machine; the grinding machine is an 8-section spiral grinding machine, and the power is 3.4 kW; the grinding is divided into coarse grinding, fine grinding and precision grinding, wherein the coarse grinding mainly grinds the rear edge C, the fine grinding mainly grinds the middle edge B, and the precision grinding mainly grinds the front edge A; the blade angles of the front edge A, the middle edge B and the rear edge C are θ(A1)=16.7°, θ(A2)=19.6°, θ(B1)=9.5°, θ(B2)=10.4°, θ(C1)=6.3° and θ(C2)=6.6° respectively; the vertical lengths of the front edge A, the middle edge B and the rear edge C are L(A1)=0.058 mm, L(A2)=0.056 mm, L(B1)=0.173 mm, L(B2)=0.084 mm, L(C1)=0.737 mm and L(C2)=0.796 mm respectively; the length of the edge inclined line of the first front edge area is smaller than the length of the edge inclined line of the second front edge area, the tip surface of the blade edge is an inclined plane, the angle between the inclined plane and the thickness direction is 8°, and the thickness of the blade tip is 0.013 mm.

[0159] Cleaning: the sectioning blade 201 is ultrasonically cleaned, the power of the ultrasonic cleaning machine is 26 kW, and the time is 7 min.

[0160] Coating and sintering: the coating is divided into two layers, the first layer is to plasma spray metal chromium on the cutting edge and blade edge of the sectioning blade 201 under vacuum or low pressure conditions, and the second layer is to uniformly spray Teflon on the cutting edge under a closed environment, and finally warm sintering; the air partial pressure of the vacuum or low pressure conditions in the first layer of chromium plating is 17 KPa, the temperature is 240℃, and the time is 7 min; the second layer of Teflon spraying is atomization spraying, the time is 6 min, and the blade edge is uniformly wrapped by Teflon at 100%, and the wrapping thickness is 0.003 mm; the sintering temperature is 650℃, and the time is 8 min.

[0161] Cutting: the sectioning blade 200 is cut into separate sectioning blades 201;

[0162] Detection and sterilization packaging: the cut blade is non-destructively detected, and then sterilized and packaged in a dust-free workshop.

[0163] The length of the tissue sectioning blade obtained in this embodiment is 80.02 mm, the width is 8.01 mm, and the thickness is 0.25 mm.

[0164] Example 4

[0165] According to the manufacturing method flow and equipment schematic diagram shown in Figure 1 to manufacture the tissue sectioning blade:

[0166] Place the blade raw material on the material rack and pull out the material belt, which is made of SUS440 stainless steel.

[0167] A slicing knife belt is obtained by punching a material strip with a 25-ton high-speed precision punch press. Slicing knives are arranged on the slicing knife belt, and the blades of each slicing knife are arranged on the same side of the slicing knife belt.

[0168] The slicing knife strip is subjected to gradient calcination treatment-freezing treatment-alcohol spraying treatment-low-temperature tempering treatment in sequence. The gradient heat treatment is divided into 4 sections, the temperature of the first section is 900°C, the temperature of the second section is 1050°C, the temperature of the third section is 1250°C, and the temperature of the fourth section is preferably 1300°C. The insulation time of the first section is 2 minutes, the insulation time of the second section is 2 minutes, the insulation time of the third section is 3 minutes, and the insulation time of the fourth section is 1 minute; the temperature of the freezing treatment is -90°C and the time is 8 minutes; the alcohol spraying time is 0.7 minutes; the temperature of the low-temperature tempering treatment is 295°C and the time is preferably 10 minutes; the gradient heat treatment and the low-temperature tempering treatment are both carried out under the protection of 99.99% high-purity nitrogen.

[0169] The blade of the heat-treated slicing knife belt is ground using a knife grinder; the knife grinder is an 8-stage spiral knife grinder with a power of 3.5kW: grinding is divided into coarse grinding, fine grinding and fine grinding, among which coarse grinding mainly grinds the rear front surface C, fine grinding mainly grinds the center front surface B, and fine grinding mainly grinds the front surface A. The blade angles of the front surface A, the center surface B and the back surface C are: θ(A1)=16.7°, θ(A2)=19.3°, θ(B1)=9.4°, θ(B2)=10.8°, θ(C1)=6.5° and θ(C2)=6.6° respectively; the vertical lengths of the front surface A, the center surface B and the back surface C are: L(A1)=0.052mm, L(A2)=0.056mm, L(B1)=0.175mm, L(B2)=0.084mm, L(C1)=0.733mm and L(C2)=0.796mm respectively; the length of the edge oblique line of the first front area is smaller than the length of the edge oblique line of the second front area, the tip surface of the blade is an inclined plane, the angle between the inclined plane and the thickness direction is 10°, and the thickness of the blade tip is 0.011mm.

[0170] Cleaning: The slicer 201 is ultrasonically cleaned with an ultrasonic cleaning machine having a power of 27 kW and a cleaning time of 9 minutes.

[0171] Coating and sintering: The coating is divided into two layers. The first layer is to plasma thermally spray metal chromium on the blade and edge of the slicing knife 201 under vacuum or low pressure conditions. The second layer is to evenly spray Teflon on the cutting edge in a closed environment, and finally heated and sintered to form. The air partial pressure of the vacuum or low pressure conditions in the first layer of chromium plating is 18KPa, the temperature is 270℃, and the time is 9 minutes. The second layer is sprayed with Teflon, and the spraying method is atomized spraying for 8 minutes to ensure that the blade edge is 100% evenly covered with Teflon, with a wrapping thickness of 0.003mm. The temperature of the sintering is 600℃ and the time is 9 minutes.

[0172] Cutting: cutting the slicing blade belt 200 into separate slicing blades 201;

[0173] Inspection and sterilization packaging: The cut blades are subjected to non-destructive inspection and then sterilized and packaged in a dust-free workshop.

[0174] The tissue slicer obtained in this embodiment has a length of 80.02 mm, a width of 8.01 mm, and a thickness of 0.254 mm.

[0175] Example 5

[0176] according to Figure 1 The manufacturing method process and equipment schematic diagram shown are for manufacturing tissue slice knives:

[0177] Place the blade raw material on the material rack and pull out the material belt, which is made of SUS440 stainless steel.

[0178] A slicing knife belt is obtained by punching a material strip with a 45-ton high-speed precision punch press. Slicing knives are arranged on the slicing knife belt, and the blades of each slicing knife are arranged on the same side of the slicing knife belt.

[0179] The slicing knife strip is subjected to gradient calcination treatment-freezing treatment-alcohol spraying treatment-low-temperature tempering treatment in sequence. The gradient heat treatment is divided into 4 sections, the temperature of the first section is 1000°C, the temperature of the second section is 1050°C, the temperature of the third section is 1250°C, and the temperature of the fourth section is preferably 1300°C. The insulation time of the first section is 2 minutes, the insulation time of the second section is 2 minutes, the insulation time of the third section is 3 minutes, and the insulation time of the fourth section is 1 minute; the temperature of the freezing treatment is -100°C, and the time is 7 minutes; the alcohol spraying time is 1 minute; the temperature of the low-temperature tempering treatment is 310°C, and the time is preferably 15 minutes; the gradient heat treatment and the low-temperature tempering treatment are both carried out under the protection of 99.99% high-purity nitrogen.

[0180] The blade of the heat-treated slicing knife belt is ground using a knife grinder; the knife grinder is an 8-stage spiral knife grinder with a power of 3.7kW: grinding is divided into coarse grinding, fine grinding and fine grinding, among which coarse grinding mainly grinds the rear front surface C, fine grinding mainly grinds the center front surface B, and fine grinding mainly grinds the front surface A. The blade angles of the front surface A, the center surface B and the back surface C are: θ(A1)=16.5°, θ(A2)=19.7°, θ(B1)=9.5°, θ(B2)=10.4°, θ(C1)=6.5° and θ(C2)=6.7° respectively; the vertical lengths of the front surface A, the center surface B and the back surface C are: L(A1)=0.054mm, L(A2)=0.051mm, L(B1)=0.177mm, L(B2)=0.089mm, L(C1)=0.733mm and L(C2)=0.791mm respectively; the length of the edge oblique line of the first front area is smaller than the length of the edge oblique line of the second front area, the tip surface of the blade is an inclined plane, the angle between the inclined plane and the thickness direction is 8°, and the thickness of the blade tip is 0.013mm.

[0181] Cleaning: The slicer 201 is ultrasonically cleaned with an ultrasonic cleaning machine having a power of 20 kW and a cleaning time of 10 minutes.

[0182] Coating and sintering: The coating is divided into two layers. The first layer is to plasma thermally spray metal chromium on the blade and edge of the slicing knife 201 under vacuum or low pressure conditions. The second layer is to evenly spray Teflon on the cutting edge in a closed environment, and finally heated and sintered to form. The air partial pressure of the vacuum or low pressure conditions in the first layer of chromium plating is 20KPa, the temperature is 350℃, and the time is 5 minutes. The second layer is sprayed with Teflon, and the spraying method is atomized spraying for 5 minutes to ensure that the blade edge is 100% evenly covered with Teflon, with a wrapping thickness of 0.001mm. The temperature of the sintering is 600℃ and the time is 10 minutes.

[0183] Cutting: cutting the slicing blade belt 200 into separate slicing blades 201;

[0184] Inspection and sterilization packaging: The cut blades are subjected to non-destructive inspection and then sterilized and packaged in a dust-free workshop.

[0185] The tissue slicer obtained in this embodiment has a length of 80.03 mm, a width of 8.07 mm, and a thickness of 0.252 mm.

[0186] Example 6

[0187] according to Figure 1 The manufacturing method process and equipment schematic diagram shown are for manufacturing tissue slice knives:

[0188] Place the blade raw material on the material rack and pull out the material belt, which is made of SUS440 stainless steel.

[0189] A 30-ton high-speed precision punch press is used to punch a material strip to obtain a slicing knife strip. Slicing knives are arranged on the slicing knife strip, and the blades of each slicing knife are arranged on the same side of the slicing knife strip.

[0190] The slicing knife strip is subjected to gradient calcination treatment-freezing treatment-alcohol spraying treatment-low-temperature tempering treatment in sequence. The gradient heat treatment is divided into 4 sections, the temperature of the first section is 900°C, the temperature of the second section is 1150°C, the temperature of the third section is 1250°C, and the temperature of the fourth section is preferably 1300°C. The insulation time of the first section is 1.5 minutes, the insulation time of the second section is 2.5 minutes, the insulation time of the third section is 1.5 minutes, and the insulation time of the fourth section is 1.5 minutes; the temperature of the freezing treatment is -90°C and the time is 7 minutes; the alcohol spraying time is 0.6 minutes; the temperature of the low-temperature tempering treatment is 310°C and the time is preferably 15 minutes; the gradient heat treatment and the low-temperature tempering treatment are both carried out under the protection of 99.99% high-purity nitrogen.

[0191] The blade of the heat-treated slicing knife belt is ground using a knife grinder; the knife grinder is an 8-stage spiral knife grinder with a power of 4kW: the grinding is divided into coarse grinding, fine grinding and fine grinding, among which coarse grinding mainly grinds the rear front surface C, fine grinding mainly grinds the center front surface B, and fine grinding mainly grinds the front surface A. The blade angles of the front surface A, the center surface B and the back surface C are: θ(A1)=16.7°, θ(A2)=19.4°, θ(B1)=9.5°, θ(B2)=10.7°, θ(C1)=6.6° and θ(C2)=6.4° respectively; the vertical lengths of the front surface A, the center surface B and the back surface C are: L(A1)=0.052mm, L(A2)=0.055mm, L(B1)=0.175mm, L(B2)=0.087mm, L(C1)=0.731mm and L(C2)=0.795mm respectively; the length of the edge oblique line of the first front area is smaller than the length of the edge oblique line of the second front area, the tip surface of the blade is an inclined plane, the angle between the inclined plane and the thickness direction is 7°, and the thickness of the blade tip is 0.012mm.

[0192] Cleaning: The slicer 201 is ultrasonically cleaned with an ultrasonic cleaning machine having a power of 28 kW and a cleaning time of 10 minutes.

[0193] Coating and sintering: The coating is divided into two layers. The first layer is to plasma thermally spray metal chromium on the blade and edge of the slicing knife 201 under vacuum or low pressure conditions. The second layer is to evenly spray Teflon on the cutting edge in a closed environment, and finally heated and sintered to form. The air partial pressure of the vacuum or low pressure conditions in the first layer of chromium plating is 20KPa, the temperature is 350℃, and the time is 10 minutes. The second layer is sprayed with Teflon, and the spraying method is atomized spraying for 7 minutes to ensure that the blade edge is 100% evenly covered with Teflon, with a wrapping thickness of 0.003mm. The sintering temperature is 600℃ and the time is 12 minutes.

[0194] Cutting: cutting the slicing blade belt 200 into separate slicing blades 201;

[0195] Inspection and sterilization packaging: The cut blades are subjected to non-destructive inspection and then sterilized and packaged in a dust-free workshop.

[0196] The tissue slicer obtained in this embodiment has a length of 80.05 mm, a width of 8.01 mm, and a thickness of 0.25 mm.

[0197] Example 7

[0198] according to Figure 1 The manufacturing method process and equipment schematic diagram shown are for manufacturing tissue slice knives:

[0199] Place the blade raw material on the material rack and pull out the material belt, which is made of SUS420 stainless steel.

[0200] A material strip is punched with a 40-ton high-speed precision punch press to obtain a slicing knife strip, on which slicing knives are arranged, and the blades of each slicing knife are arranged on the same side of the slicing knife strip.

[0201] The slicing knife strip is subjected to gradient calcination treatment-freezing treatment-alcohol spraying treatment-low-temperature tempering treatment in sequence. The gradient heat treatment is divided into 4 sections, the temperature of the first section is 1000°C, the temperature of the second section is 1040°C, the temperature of the third section is 1240°C, and the temperature of the fourth section is preferably 1340°C. The insulation time of the first section is 2 minutes, the insulation time of the second section is 3 minutes, the insulation time of the third section is 1 minute, and the insulation time of the fourth section is 1 minute; the temperature of the freezing treatment is -90°C and the time is 7 minutes; the alcohol spraying time is 0.81 minutes; the temperature of the low-temperature tempering treatment is 295°C and the time is preferably 13 minutes; the gradient heat treatment and the low-temperature tempering treatment are both carried out under the protection of 99.99% high-purity nitrogen.

[0202] The blade of the heat-treated slicing knife belt is ground using a knife grinder; the knife grinder is an 8-stage spiral knife grinder with a power of 3.2kW: grinding is divided into coarse grinding, fine grinding and fine grinding, among which coarse grinding mainly grinds the rear front surface C, fine grinding mainly grinds the center front surface B, and fine grinding mainly grinds the front surface A. The blade angles of the front surface A, the center surface B and the back surface C are: θ(A1)=16.7°, θ(A2)=19.6°, θ(B1)=9.5°, θ(B2)=10.5°, θ(C1)=6.6° and θ(C2)=6.7° respectively; the vertical lengths of the front surface A, the center surface B and the back surface C are: L(A1)=0.056mm, L(A2)=0.055mm, L(B1)=0.174mm, L(B2)=0.087mm, L(C1)=0.733mm and L(C2)=0.793mm respectively; the length of the edge oblique line of the first front area is smaller than the length of the edge oblique line of the second front area, the tip surface of the blade is an inclined plane, the angle between the inclined plane and the thickness direction is 5.4°, and the thickness of the blade tip is 0.014mm.

[0203] Cleaning: The slicer 201 is ultrasonically cleaned with an ultrasonic cleaning machine having a power of 25 kW and a cleaning time of 8 minutes.

[0204] Coating and sintering: The coating is divided into two layers. The first layer is to plasma thermally spray metal chromium on the blade and edge of the slicing knife 201 under vacuum or low pressure conditions. The second layer is to evenly spray Teflon on the cutting edge in a closed environment, and finally heated and sintered to form. The air partial pressure of the vacuum or low pressure conditions in the first layer of chromium plating is 17KPa, the temperature is 300℃, and the time is 9 minutes. The second layer is sprayed with Teflon, and the spraying method is atomized spraying for 9 minutes to ensure that the blade edge is 100% evenly covered with Teflon, with a wrapping thickness of 0.003mm. The temperature of the sintering is 600℃ and the time is 10 minutes.

[0205] Cutting: cutting the slicing blade belt 200 into separate slicing blades 201;

[0206] Inspection and sterilization packaging: The cut blades are subjected to non-destructive inspection and then sterilized and packaged in a dust-free workshop.

[0207] The tissue slicer obtained in this embodiment has a length of 80.05 mm, a width of 8.01 mm, and a thickness of 0.248 mm.

[0208] Test Example 1

[0209] The tissue slicers prepared in Examples 1 to 7 of the present invention were subjected to material and performance analysis, and the results are shown in Tables 1 and 2:

[0210] Table 1 Material analysis of tissue slicers prepared in Examples 1 to 7

[0211] Example Fe(%) Cr(％) Mn (%) C(％) Si (%) Ni(%) V(％) Cu(%) other(%) 1 84.95 13.1 0.77 0.63 0.29 0.091 0.042 0.023 0.104 2 84.97 12.9 0.76 0.65 0.31 0.093 0.044 0.022 0.251 3 84.94 13 0.79 0.67 0.28 0.094 0.043 0.019 0.164 4 84.96 13.1 0.73 0.62 0.3 0.092 0.042 0.024 0.132 5 84.92 12.8 0.75 0.63 0.32 0.095 0.041 0.021 0.423 6 84.93 13.1 0.74 0.66 0.29 0.091 0.043 0.023 0.123 7 84.99 12.9 0.79 0.68 0.31 0.097 0.046 0.025 0.162

[0212] From Table 1, it can be found that the main components of the tissue slice knives prepared in Examples 1 to 7 are Fe, Cr, Mn, C and Si, which meet the requirements for blade preparation.

[0213] Table 2 Performance analysis table of tissue slice knives prepared in Examples 1 to 7.

[0214] Example Hardness (HRC) Salt spray time (h) Paper cutting force (N) Toughness (MPa) Blade gloss (GU) 1 58.7 72 18 325 98 2 58.4 71 17 324 96 3 57.5 72 18 325 97 4 57.9 70 17 323 98 5 58.1 69 16 322 95 6 58.6 71 18 325 98 7 58.8 72 17 324 96

[0215] From Table 2, it can be found that the Rockwell hardness of the slicing knives prepared in Examples 1 to 7 is 57.5 to 58.8, the salt spray time is 69 to 72 h, the paper cutting force is 16 to 18 N, the toughness is 322 to 325 N, and the blade gloss (measured by a 20-degree gloss meter) is 95 to 98 GU. Compared with commercially available imported blades, the slicing knives prepared in the present invention have good hardness, are not easy to rust, and have better sharpness, toughness and blade gloss.

[0216] Test Example 2

[0217] The tissue slicer prepared in Example 1 of the present invention was applied to bone tissue for frozen sectioning. The results of bone tissue sectioning are as follows: Figure 5 shown.

[0218] from Figure 5 It is not difficult to find that by using the tissue slicer prepared in Example 1 of the present invention to slice bone tissue, experimental slices with moderate thickness and smooth incisions can be obtained, and repeated tests on the blades show that the slicer prepared in the present invention can be reused 30 to 35 times.

[0219] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A tissue slicer, comprising a blade body, a blade edge and a blade tip, characterized in that: Along the length direction of the tissue slicer (201), the blade (206) is provided with a front area (A), a middle peak area (B) and a rear front area (C); along the central axis of the thickness of the tissue slicer (201), the front area (A) is provided with a first front area (A1) and a second front area (A2) located on both sides of the central axis; a first blade angle formed by an edge of the first front area (A1) and the central axis is smaller than a second blade angle formed by an edge of the second front area (A2) and the central axis; Along the central axis of the thickness of the tissue slicer (201), the central peak area (B) is provided with a first central peak area (B1) and a second central peak area (B2) located on both sides of the central axis, and a third blade angle formed by an edge of the first central peak area (B1) and the central axis is smaller than a fourth blade angle formed by an edge of the second central peak area (B2) and the central axis; Along the central axis of the thickness of the tissue slicing knife (201), the back edge region (C) is provided with a first back edge region (C1) and a second back edge region (C2) located on both sides of the central axis, and a fifth blade angle formed by an edge of the first back edge region (C1) and the central axis is greater than a sixth blade angle formed by an edge of the second back edge region (C2) and the central axis; The first blade angle, the third blade angle and the fifth blade angle decrease in sequence; the second blade angle, the fourth blade angle and the sixth blade angle decrease in sequence; The first vertex of the first cutting edge angle on the central axis is located at the same position as the second vertex of the second cutting edge angle on the central axis; Taking the first vertex as the starting position and the point where the edge end of the first front area (A1) is projected onto the central axis as the ending position, the length of the starting position and the ending position is the vertical length of the first front area (A1); the length where the edge of the first middle peak area (B1) is projected onto the central axis is the vertical length of the first middle peak area (B1); the length where the edge of the first rear front area (C1) is projected onto the central axis is the vertical length of the first rear front area (C1); The second vertex is taken as the starting position, and the point where the edge end of the second front area (A2) is projected onto the central axis is taken as the ending position. The length of the starting position and the ending position is the vertical length of the second front area (A2); the length where the edge of the second middle peak area (B2) is projected onto the central axis is the vertical length of the second middle peak area (B2); and the length where the edge of the second rear front area (C2) is projected onto the central axis is the vertical length of the second rear front area (C2). The vertical length of the second front area (A2), the vertical length of the first front area (A1), the vertical length of the second middle peak area (B2), the vertical length of the first middle peak area (B1), the vertical length of the first rear area (C1) and the vertical length of the second rear area (C2) increase in sequence; The top end surface of the tip of the blade (206) is an inclined plane, and the angle between the first projection line of the inclined plane in the plane formed by the thickness direction and the length direction and the thickness direction is 5 to 10 degrees.

2. The tissue slicer according to claim 1, characterized in that The thickness of the tip is 0.01±0.005 mm.

3. The tissue slicer according to claim 1, characterized in that: The intersection of the second projection line and the first projection line in the plane formed by the edge of the second front area (A2) in the thickness direction and the length direction is taken as the vertex, and the angle between the first projection line and the thickness direction is 5 to 10 degrees.

4. The method for manufacturing a tissue slicer according to any one of claims 1 to 3, characterized in that: The following steps are involved: The raw materials of the tissue slicer are sequentially loaded and pulled to obtain a discharge belt; The discharge belt is punched and formed to obtain a slicing knife belt having a knife body and a blade structure; The slicing knife band is sequentially subjected to a gradient calcination treatment, a freezing treatment, and a low-temperature tempering treatment to obtain a heat-treated knife band, wherein the gradient calcination treatment and the low-temperature tempering treatment are both performed in a protective gas; Grinding the blade of the heat-treated knife band to obtain a tissue slice knife prototype; The tissue slicer prototype is sequentially cleaned, coated, sintered and cut to obtain the tissue slicer.

5. The manufacturing method according to claim 4, characterized in that The pressure of the stamping forming is 25 to 45 tons.

6. The manufacturing method according to claim 4, characterized in that The gradient calcination treatment includes a first calcination treatment, a second calcination treatment, a third calcination treatment and a fourth calcination treatment in sequence; the holding temperature of the first calcination treatment is 900-1000°C, and the holding time of the first calcination treatment is 1-2 minutes; the holding temperature of the second calcination treatment is 1050-1150°C, and the holding time of the second calcination treatment is 2-3 minutes; the holding temperature of the third calcination treatment is 1200-1250°C, and the holding time of the third calcination treatment is 1-3 minutes; the holding temperature of the fourth calcination treatment is 1300-1350°C, and the holding time of the fourth calcination treatment is 1-2 minutes.

7. The manufacturing method according to claim 4, characterized in that The holding temperature of the freezing treatment is -80 to -100°C, and the holding time of the freezing treatment is 5 to 10 minutes; The holding temperature of the low-temperature tempering treatment is 290-310° C., and the holding time of the low-temperature tempering treatment is 8-15 minutes.

8. The manufacturing method according to claim 5, characterized in that The grinding adopts an 8-stage spiral sharpening machine; the grinding power is 3-4kW; the grinding includes a coarse grinding rear edge zone (C), a fine grinding middle edge zone (B) and a fine grinding front edge zone (A).

9. The manufacturing method according to claim 5, characterized in that The freezing treatment obtains a frozen knife belt. Before the low-temperature tempering treatment, the frozen knife belt is subjected to an alcohol spray treatment to obtain a sprayed knife belt, and the sprayed knife belt is subjected to the low-temperature tempering treatment. The alcohol spray treatment time is 0.5 to 1 minute.

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