A PCR well plate film sealer

Abstract

The application relates to the technical field of medical equipment, in particular to a PCR (Polymerase Chain Reaction) hole plate film sealing instrument, which comprises a bottom plate, an upper plate, a first driving unit, a carrier table, a gantry, a second driving unit and a roller assembly. The upper plate is arranged above the bottom plate; the carrier table is arranged above the upper plate; the first driving unit is arranged on the bottom plate and the upper plate, and the first driving unit is connected with the carrier table; the gantry is fixed on the upper plate, and the second driving unit is arranged on the upper plate; the second driving unit is connected with the roller assembly; and the roller assembly is located directly above the carrier table. The PCR hole plate film sealing instrument places a PCR reaction plate and a pressure-sensitive film on an adapter, and the adapter is arranged on the carrier table; the second driving unit drives the roller assembly to longitudinally run, so that the pressure-sensitive film is pressed against the PCR reaction plate; the first driving unit drives the carrier table to reciprocate transversely, so that the carrier table drives the adapter and the PCR reaction plate above the adapter to reciprocate transversely, the pressure-sensitive film is sealed on the PCR reaction plate, and reliable film sealing of the pressure-sensitive film is realized.

Description

Technical Field

[0001] This invention relates to the field of medical equipment technology, and in particular to a PCR plate sealing device. Background Technology

[0002] PCR, short for Polymerase Chain Reaction, is an enzymatic chemical reaction that can amplify a target gene to be tested by 500,000 to 1 million times in a very short time in a test tube, greatly improving the sensitivity of gene diagnosis and reducing the difficulty of analysis. After collecting samples, PCR plates need to be sealed.

[0003] Currently, the mainstream PCR sealing instrument on the market is heat-sealing film. Although PCR heat-sealing film is relatively widely used, it still has some shortcomings: First, it cannot handle pressure-sensitive film encapsulation; second, the temperature of the heat-sealing plate is very high during the heat-sealing process, which may affect the environment of the reagent samples in the well plate and cause evaporation when it is close to the PCR reaction plate; third, the heat-sealing instrument is in a high-temperature environment for a long time, which causes the internal components to age quickly and the instrument's lifespan to decrease sharply. Summary of the Invention

[0004] This invention addresses the technical problem of reliably sealing pressure-sensitive membranes onto PCR reaction plates in existing technologies by providing a PCR plate sealing instrument.

[0005] The technical solution of the present invention to solve the above-mentioned technical problems is as follows:

[0006] A PCR plate sealing device includes: a base plate, an upper plate, a first drive unit, a stage, a gantry frame, a second drive unit, and a roller assembly;

[0007] The upper plate is disposed above the bottom plate; the platform is disposed above the upper plate;

[0008] The first drive unit is disposed on the base plate and the top plate, and is connected to the platform to drive the platform to move laterally.

[0009] The gantry frame is fixed on the upper plate, and the second drive unit is disposed on the upper plate; the second drive unit is connected to the roller assembly and is used to drive the roller assembly to move longitudinally; the roller assembly is located directly above the platform.

[0010] Furthermore, the first drive unit includes: a Y motor assembly, a synchronous belt torque amplification structure, a synchronous belt drive structure, and a sliding assembly;

[0011] The Y motor assembly is connected to the upper plate;

[0012] The synchronous belt torque amplification structure is disposed on the base plate and the upper plate, and the synchronous belt torque amplification structure is connected to the sliding component;

[0013] The sliding component is disposed on the upper plate and can move laterally along the upper plate; the stage is connected to the sliding component.

[0014] Furthermore, the synchronous belt torque amplification structure includes: a first synchronous pulley, a first synchronous belt, a first rotating shaft, a second synchronous pulley, a connecting cylinder, and a third synchronous pulley;

[0015] The first synchronous pulley is connected to the output end of the Y motor assembly;

[0016] The connecting cylinder passes through the upper plate and is fixedly connected to the upper plate; the first rotating shaft passes through the connecting cylinder and is rotatably connected to the connecting cylinder; the second synchronous pulley is installed at the upper end of the first rotating shaft; the first synchronous pulley is connected to the second synchronous pulley through the first synchronous belt; the third synchronous pulley is installed at the lower end of the first rotating shaft and is connected to the synchronous belt drive structure.

[0017] Furthermore, the synchronous belt drive structure includes: a second synchronous belt, a second rotating shaft, and a fourth synchronous pulley;

[0018] The second rotating shaft is fixed to the upper end of the base plate, and the fourth synchronous pulley is mounted on the third rotating shaft and can rotate around the second rotating shaft;

[0019] The third synchronous pulley is connected to the fourth synchronous pulley via the second synchronous belt;

[0020] The second synchronous belt is connected to the sliding mechanism.

[0021] Furthermore, the sliding assembly includes: a first slider, a first linear guide rail, a timing belt connecting block, a pressure plate, a first zero-position contact plate, and a first sensor;

[0022] The upper plate has a through groove running vertically through it;

[0023] The groove has parallel first linear guide rails on both sides; the first sliders are respectively provided on the first linear guide rails; the stage is fixed on the first sliders.

[0024] The timing belt connecting block passes through the groove and is fixedly connected to the bottom of the stage;

[0025] The second synchronous belt is fixed to the synchronous belt connecting block by the pressure plate;

[0026] The first zero-position contact plate is disposed on the stage, and the first sensor is disposed on the upper plate, with the first zero-position contact plate cooperating with the first sensor.

[0027] Furthermore, the second drive unit includes: a lead screw motor, a motor mounting plate, a second slider, a guide plate, and a second linear guide;

[0028] The guide rail plate is longitudinally fixed to the gantry frame; the second linear guide rail is longitudinally fixed to the guide rail plate; the second slider is disposed on the second linear guide rail;

[0029] The motor mounting plate is fixed to the upper end of the guide rail plate; the lead screw motor is fixed to the motor mounting plate; the output end of the lead screw motor is connected to the roller assembly and is used to drive the roller assembly to move longitudinally; the roller assembly is fixedly connected to the second slider.

[0030] Furthermore, the roller assembly includes: an assembly mounting plate, a lead screw nut seat, a first guide shaft, a second guide shaft, a first rectangular spring, a second rectangular spring, a roller mounting bracket, a roller, a second zero-position contact plate, and a second sensor;

[0031] The lead screw nut seat is fixed on the component mounting plate, and the output end of the lead screw motor is connected to the lead screw nut seat;

[0032] One end of the first guide shaft and one end of the second guide shaft are fixed to the component mounting plate. The roller mounting bracket has a first guide hole and a second guide hole. The other end of the first guide shaft is disposed in the first guide hole, and the other end of the second guide shaft is disposed in the second guide hole. A first rectangular spring is disposed on the first guide shaft. One end of the first rectangular spring is connected to the component mounting plate, and the other end of the first rectangular spring is connected to the roller mounting bracket. A second rectangular spring is disposed on the second guide shaft. One end of the second rectangular spring is connected to the component mounting plate, and the other end of the second rectangular spring is connected to the roller mounting bracket.

[0033] The roller is rotatably connected to the roller mounting bracket;

[0034] The second zero-position contact plate is disposed on the component mounting plate, and the second sensor is disposed on one side of the component mounting plate. The second zero-position contact plate cooperates with the component mounting plate.

[0035] Furthermore, the roller includes a shaft core; the shaft core has a cylindrical structure, and the outside of the shaft core is covered with polyurethane antistatic adhesive.

[0036] Furthermore, the first synchronous pulley is a 15-tooth 3M synchronous pulley; the second, third, and fourth synchronous pulleys are 45-tooth 3M synchronous pulleys.

[0037] Furthermore, the platform is provided with positioning pins that cooperate with the adapter.

[0038] The PCR plate sealing instrument provided by this invention has at least the following beneficial effects or advantages:

[0039] The PCR plate sealing apparatus provided by this invention comprises an upper plate positioned above a base plate; a stage positioned above the upper plate; a first drive unit positioned on both the base plate and the upper plate, connected to the stage, for driving the stage to move laterally; a gantry frame fixed to the upper plate; and a second drive unit positioned on the upper plate; the drive units connected to a roller assembly for driving the roller assembly to move longitudinally; the roller assembly is located directly above the stage. In this PCR plate sealing apparatus, the PCR reaction plate and pressure-sensitive membrane are placed on an adapter, which is then placed on the stage. The second drive unit drives the roller assembly to move longitudinally, pressing the pressure-sensitive membrane against the PCR reaction plate. Furthermore, the first drive unit drives the stage to reciprocate laterally, causing the stage to move the adapter and the PCR reaction plate above it to reciprocate laterally, sealing the pressure-sensitive membrane on the PCR reaction plate and achieving reliable sealing of the pressure-sensitive membrane. Attached Figure Description

[0040] Figure 1 This is a schematic diagram of the PCR plate sealing device provided in an embodiment of the present invention;

[0041] Figure 2 This is a schematic diagram of the structure of the first driving unit provided in an embodiment of the present invention;

[0042] Figure 3 This is a schematic diagram of the synchronous belt torque amplification structure and synchronous belt drive structure provided in the embodiments of the present invention;

[0043] Figure 4 This is a schematic diagram of the sliding component structure provided in an embodiment of the present invention;

[0044] Figure 5 This is a schematic diagram of the second driving unit structure provided in an embodiment of the present invention;

[0045] Figure 6 This is a schematic diagram of the roller assembly structure provided in an embodiment of the present invention.

[0046] The attached diagram lists the components represented by each number as follows:

[0047] 1-Base plate, 2-Support shaft, 3-Synchronous belt drive structure, 4-Upper plate, 5-Synchronous belt torque amplification structure, 6-Y motor assembly, 7-Gantry frame, 8-Guide rail plate, 9-Motor mounting plate, 10-Screw motor, 11-Component mounting plate, 12-Rectangular spring, 13-First guide shaft, 14-Roller, 15-Adapter, 16-Platform, 17-First sensor, 18-First linear guide rail, 19-Roller, 20-Positioning pin, 21-First zero-position contact plate, 22-Second rotating shaft, 23-Fourth synchronous belt pulley, 24-Second synchronous belt, 25-Third synchronous belt pulley, 26-Connecting cylinder, 27-Second synchronous belt pulley, 28-First rotating shaft, 29-First synchronous belt, 30-First synchronous belt pulley, 31-Synchronous belt connecting block, 32-Pressure plate, 33-Second sensor, 34-Second linear guide rail, 35-Screw nut seat. Detailed Implementation

[0048] This invention addresses the technical problem of reliably sealing pressure-sensitive membranes onto PCR reaction plates in existing technologies by providing a PCR plate sealing instrument.

[0049] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0050] In the description of this invention, it should be noted that the terms used in the various embodiments, such as "upper," "lower," "front," "rear," "left," and "right," which indicate orientation, are only used to simplify the description of the positional relationships based on the accompanying drawings and do not mean that the components and devices referred to must be operated in accordance with the specific orientations and defined operations, methods, and structures in the specification. Such directional terms do not constitute a limitation of this invention.

[0051] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections or detachable connections; they can refer to direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention in light of the specific circumstances.

[0052] like Figure 1 This invention provides a PCR plate sealing device, comprising: a base plate 1, an upper plate 4, a first drive unit, a stage 16, a gantry frame 7, a second drive unit, and a roller assembly. Wherein:

[0053] Both the upper plate 4 and the bottom plate 1 are flat plate structures. The upper surfaces of the upper plate 4 and the bottom plate 1 are horizontal and smooth. The upper plate 4 is set above the bottom plate 1. The lower end of the upper plate 4 is fixed to the upper end of the bottom plate 1 by the support shaft 2. The lower end of the bottom plate 1 is provided with multiple symmetrically distributed support legs.

[0054] The platform 16 is a flat plate structure, positioned above the upper plate 4. A first drive unit is mounted on both the base plate 1 and the upper plate 4, connected to the platform 16, and used to drive the platform 16 to move laterally. A gantry frame 7 is fixed to the upper plate 4, and a second drive unit is mounted on the upper plate 4; the second drive unit is connected to the roller assembly, and used to drive the roller assembly to move longitudinally; the roller assembly is located directly above the platform 16. Rollers 19 can be installed at the bottom of the platform 16, positioned at the upper end of the upper plate 4 to reduce movement resistance.

[0055] Specifically, such as Figure 2 The first drive unit includes: a Y-motor assembly 6, a synchronous belt torque amplification structure 5, a synchronous belt drive structure 3, and a sliding assembly. The Y-motor assembly 6 is connected to the upper plate 4; the synchronous belt torque amplification structure 5 is disposed on the base plate 1 and the upper plate 4, and is connected to the sliding assembly. The sliding assembly is disposed on the upper plate 4 and can move laterally along the upper plate 4; the stage 16 is connected to the sliding assembly.

[0056] Based on the above scheme, such as Figure 1 and Figure 3 The synchronous belt torque amplification structure 5 includes: a first synchronous pulley 30, a first synchronous belt 29, a first rotating shaft 28, a second synchronous pulley 27, a connecting cylinder 26, and a third synchronous pulley 25. The first synchronous pulley 30 is connected to the output end of the Y motor assembly 6, and the Y motor assembly 6 drives the first synchronous pulley 30 to rotate. The connecting cylinder 26 passes through the upper plate 4 and is fixedly connected to the upper plate 4; the first rotating shaft 28 passes through the connecting cylinder 26 and is rotatably connected to the connecting cylinder 26; the second synchronous pulley 27 is installed at the upper end of the first rotating shaft 28; the first synchronous pulley 30 is connected to the second synchronous pulley 27 through the first synchronous belt 29, and during rotation, the first synchronous pulley 30 drives the second synchronous pulley 27 to rotate through the first synchronous belt 29, and the second synchronous pulley 27 drives the first rotating shaft 28 to rotate. The third synchronous pulley 25 is installed at the lower end of the first rotating shaft 28 and is connected to the synchronous belt drive structure 3; during rotation, the first rotating shaft 28 drives the synchronous belt drive structure 3 to rotate through the third synchronous pulley 25.

[0057] Based on the above scheme, such as Figure 1 and Figure 3The synchronous belt drive structure 3 includes: a second synchronous belt 24, a second rotating shaft 22, and a fourth synchronous pulley 23. The second rotating shaft 22 is fixed to the upper end of the base plate 1, and the fourth synchronous pulley 23 is mounted on the second rotating shaft 22. A third synchronous pulley 25 is connected to the fourth synchronous pulley 23 via the second synchronous belt 24; the second synchronous belt 24 is connected to a sliding mechanism; the third synchronous pulley 25 drives the fourth synchronous pulley 23 to rotate via the second synchronous belt 24, and the second synchronous belt 24 causes the sliding component to move during rotation.

[0058] Based on the above scheme, the sliding assembly includes: a first slider, a first linear guide rail 18, a timing belt connecting block 31, a pressure plate 32, a first zero-position contact plate 21, and a first sensor 17. A through groove is formed on the upper plate 4; parallel first linear guide rails 18 are respectively arranged on both sides of the groove; a first slider is respectively arranged on the first linear guide rail 18; the stage 16 is fixed on the first slider. The timing belt connecting block 31 passes through the groove and is fixedly connected to the bottom of the stage 16. A second timing belt 24 is fixed to the timing belt connecting block 31 by the pressure plate 32. During the forward and reverse rotation of the second timing belt 24, it drives the timing belt connecting block 31 to reciprocate linear motion, which in turn drives the first slider to reciprocate linear motion on the first linear guide rail 18, and the first slider drives the stage 16 to reciprocate linear motion. The first zero-position contact plate 21 is disposed on the stage 16, and the first sensor 17 is disposed on the upper plate 4. The first zero-position contact plate 21 and the first sensor 17 cooperate to detect the position of the stage 16.

[0059] In a preferred embodiment provided by the present invention, such as Figure 1 and Figure 5 The second drive unit includes: a lead screw motor 10, a motor mounting plate 9, a second slider, a guide rail plate 8, and a second linear guide rail 34. The guide rail plate 8 is longitudinally fixed to the gantry frame 7; the second linear guide rail 34 is longitudinally fixed to the guide rail plate 8; the second slider is disposed on the second linear guide rail 34. The motor mounting plate 9 is fixed to the upper end of the guide rail plate 8; the lead screw motor 10 is fixed to the motor mounting plate 9; the output end of the lead screw motor 10 is connected to the roller assembly for driving the longitudinal movement of the roller assembly; the roller assembly is fixedly connected to the second slider.

[0060] Based on the above plan, such as Figure 1 and Figure 6The roller assembly includes: an assembly mounting plate 11, a lead screw nut seat 35, a first guide shaft 13, a second guide shaft, a first rectangular spring 12, a second rectangular spring, a roller mounting bracket, a roller 14, a second zero-position contact plate, and a second sensor 33. The lead screw nut seat 35 is fixed to the assembly mounting plate 11. The output end of the lead screw motor 10 is connected to the lead screw nut seat 35, and the rotation of the output end of the lead screw motor 10 drives the lead screw nut seat 35 to move longitudinally. One end of the first guide shaft 13 and one end of the second guide shaft are fixed to the assembly mounting plate 11. The roller mounting bracket has a first guide hole and a second guide hole. The other end of the first guide shaft 13 is located in the first guide hole, and the other end of the second guide shaft is located in the second guide hole. The first rectangular spring 12 is mounted on the first guide shaft 13, with one end connected to the assembly mounting plate 11 and the other end connected to the roller mounting bracket. The second rectangular spring is mounted on the second guide shaft, with one end connected to the assembly mounting plate 11 and the other end connected to the roller mounting bracket. The roller 14 is rotatably connected to the roller mounting bracket. During the up-and-down movement of the lead screw nut seat 35, it drives the component mounting plate 11 to move up and down, thereby driving the roller 14 to move up and down. A rectangular spring is used in this embodiment because if the roller structure as a whole is a rigid component, after the lead screw motor 10 presses the rigid roller assembly against the load, the lead screw motor 10 operates with its own holding torque, which is very small and insufficient to provide effective pressure to complete the PCR sealing work. A rectangular spring, however, can store a large amount of axial force from the motor output, and the amount of spring compression can be customized to output different working pressures. A second zero-position contact plate is disposed on the component mounting plate 11, and a second sensor 33 is disposed on one side of the component mounting plate 11. The second zero-position contact plate and the second sensor 33 cooperate to determine the position of the roller assembly.

[0061] In this embodiment, as Figure 6 Roller 14 includes a shaft core; the shaft core has a cylindrical structure and is coated with polyurethane antistatic adhesive. The polyurethane antistatic adhesive is relatively soft and antistatic. Using this coated roller serves two purposes: first, it compensates for unevenness in the PCR reaction plate during the PCR sealing rolling process; second, the pressure-sensitive membrane and polyurethane will adhere under static electricity during rolling, and the antistatic properties of polyurethane solve this adhesion problem.

[0062] Based on the above scheme, such as Figure 1The first synchronous pulley 30 is a 15-tooth 3M synchronous pulley; the second, third, and fourth synchronous pulleys 27 and 25 are 45-tooth 3M synchronous pulleys. The synchronous belt torque amplification structure 5 consists of a 45-tooth 3M synchronous pulley, a 15-tooth 3M synchronous pulley, and a 3M synchronous belt. The 15-tooth 3M synchronous pulley is mounted on the Y-motor assembly 6, providing a power source for the transmission mechanism. Here, a 15-tooth small pulley and a 45-tooth large pulley are selected to form a 1:3 reduction and force amplification mechanism. This mechanism increases the torque output of the Y-motor assembly 6 while meeting speed requirements, providing more abundant power to the stage.

[0063] Based on the above plan, such as Figures 1-6 The stage is equipped with positioning pins 20 that mate with the adapter. In this embodiment, the PCR reaction plate adapter 15 is mounted and fixed above the stage 16 using two positioning pins 20. This adapter 15 is designed for use during the sealing process of various 96-well PCR reaction plates with different specifications, capacities, and well outer diameters currently available on the market. The adapter 15 has through holes of two different well diameters on its upper and lower sides to accommodate 96-well PCR reaction plates of different well sizes. Two positioning pin holes are made in the same position on both sides, allowing the adapter 15 to be used in either direction to accommodate PCR reaction plates of different well diameters. All four corners of the adapter 15 are chamfered to the same size for foolproof design, allowing the PCR reaction plate to be used normally even when placed blindly. The adapter has internal grooves in both the top and bottom 96 holes, which makes it compatible with both flat and skeletal PCR reaction plates. The length and width of the adapter 15 take into account the length and width data of flat / skeletal, half-skirted and full-skirted PCR reaction plates, and can accommodate reaction plates with and without skirts at the same time. The width of the flat / skeletal reaction plate is about the same as the width of the adapter. The left and right sides of the adapter 15 are recessed, and after the sealing work is completed, the recesses can accommodate fingers to easily remove the reaction plate.

[0064] like Figures 1-6 The workflow of the PCR plate sealing instrument provided in this embodiment of the invention is as follows: The user places the PCR reaction plate and pressure-sensitive membrane on the adapter 15. The Y motor assembly 6 starts and drives the stage 16 and the adapter 15 to move horizontally to a suitable position and stop. At this time, the lead screw motor 10 starts and drives the roller assembly to press down the PCR reaction plate and pressure-sensitive membrane on the adapter 15 to the set pressure. Then the Y motor assembly 6 starts and drives the stage 16 to move back and forth. During this process, the roller completes the rolling and coating process. The roller assembly returns to the zero position, and the stage 16 moves to the material pick-up and drop-off position. The sealing process ends here.

[0065] The PCR plate sealing instrument provided by this invention has at least the following beneficial effects or advantages:

[0066] The PCR plate sealing apparatus provided by this invention comprises an upper plate positioned above a base plate; a stage positioned above the upper plate; a first drive unit positioned on both the base plate and the upper plate, connected to the stage, for driving the stage to move laterally; a gantry frame fixed to the upper plate; and a second drive unit positioned on the upper plate; the drive units connected to a roller assembly for driving the roller assembly to move longitudinally; the roller assembly is located directly above the stage. In this PCR plate sealing apparatus, the PCR reaction plate and pressure-sensitive membrane are placed on an adapter, which is then placed on the stage. The second drive unit drives the roller assembly to move longitudinally, pressing the pressure-sensitive membrane against the PCR reaction plate. Furthermore, the first drive unit drives the stage to reciprocate laterally, causing the stage to move the adapter and the PCR reaction plate above it to reciprocate laterally, sealing the pressure-sensitive membrane on the PCR reaction plate and achieving reliable sealing of the pressure-sensitive membrane.

[0067] In addition, the embodiments of the present invention also have the following advantages:

[0068] The PCR plate sealing instrument provided by this invention, through the design and selection of a suitable adapter size, is compatible with various types of 96-well PCR reaction plates, exhibiting good compatibility. This sealing instrument is designed for practical use, is reversible, facilitates plate loading and unloading, and is highly user-friendly. The use of synchronous belt drive ensures high speed and low noise. The transmission device incorporates a speed reduction and force amplification structure, enabling the transmission of larger loads. Built-in sensors precisely control the movement of the stage and roller assembly, ensuring high accuracy. The synchronous belt drive offers superior safety compared to screw drives and rack and pinion drives; when a certain load torque is exceeded, the synchronous belt will slip, effectively ensuring user safety. A rectangular spring stores pressure, allowing for adjustable rolling pressures by setting the displacement of the roller rolling structure 2, providing a wide pressure adjustment range. The roller section uses a steel shaft core coated with a layer of polyurethane antistatic adhesive, which solves the problem of electrostatic adsorption of materials and compensates for the unevenness of the PCR reaction plate's surface during rolling. It has a simple structure, small size, saves laboratory workbench space, is easy to assemble and disassemble, and is simple to use.

[0069] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A PCR plate sealing instrument, characterized in that: include: Base plate, top plate, first drive unit, platform, gantry frame, second drive unit and roller assembly; The upper plate is disposed above the bottom plate; the platform is disposed above the upper plate; The first drive unit is disposed on the base plate and the top plate, and is connected to the platform to drive the platform to move laterally. The second drive unit includes: a lead screw motor, a motor mounting plate, a second slider, a guide plate, and a second linear guide; The guide rail plate is longitudinally fixed to the gantry frame; the second linear guide rail is longitudinally fixed to the guide rail plate; the second slider is disposed on the second linear guide rail; The motor mounting plate is fixed to the upper end of the guide rail plate; the lead screw motor is fixed to the motor mounting plate; the output end of the lead screw motor is connected to the roller assembly for driving the roller assembly to move longitudinally; the roller assembly is fixedly connected to the second slider. The gantry frame is fixed to the upper plate, and the second drive unit is disposed on the upper plate; the second drive unit is connected to the roller assembly and is used to drive the roller assembly to move longitudinally; the roller assembly is located directly above the platform; The roller assembly includes: an assembly mounting plate, a lead screw nut seat, a first guide shaft, a second guide shaft, a first rectangular spring, a second rectangular spring, a roller mounting bracket, a roller, a second zero-position contact plate, and a second sensor; The lead screw nut seat is fixed on the component mounting plate, and the output end of the lead screw motor is connected to the lead screw nut seat; One end of the first guide shaft and one end of the second guide shaft are fixed to the component mounting plate. The roller mounting bracket has a first guide hole and a second guide hole. The other end of the first guide shaft is disposed in the first guide hole, and the other end of the second guide shaft is disposed in the second guide hole. A first rectangular spring is disposed on the first guide shaft. One end of the first rectangular spring is connected to the component mounting plate, and the other end of the first rectangular spring is connected to the roller mounting bracket. A second rectangular spring is disposed on the second guide shaft. One end of the second rectangular spring is connected to the component mounting plate, and the other end of the second rectangular spring is connected to the roller mounting bracket. The roller is rotatably connected to the roller mounting bracket; The second zero-position contact plate is disposed on the component mounting plate, and the second sensor is disposed on one side of the component mounting plate. The second zero-position contact plate cooperates with the component mounting plate.

2. The PCR plate sealing instrument according to claim 1, characterized in that: The first drive unit includes: a Y motor assembly, a synchronous belt torque amplification structure, a synchronous belt drive structure, and a sliding assembly; The Y motor assembly is connected to the upper plate; The synchronous belt torque amplification structure is disposed on the base plate and the upper plate, and the synchronous belt torque amplification structure is connected to the sliding component; The sliding component is disposed on the upper plate and can move laterally along the upper plate; the stage is connected to the sliding component.

3. The PCR plate sealing instrument according to claim 2, characterized in that: The synchronous belt torque amplification structure includes: a first synchronous pulley, a first synchronous belt, a first rotating shaft, a second synchronous pulley, a connecting cylinder, and a third synchronous pulley; The first synchronous pulley is connected to the output end of the Y motor assembly; The connecting cylinder passes through the upper plate and is fixedly connected to the upper plate; the first rotating shaft passes through the connecting cylinder and is rotatably connected to the connecting cylinder; the second synchronous pulley is installed at the upper end of the first rotating shaft; the first synchronous pulley is connected to the second synchronous pulley through the first synchronous belt; the third synchronous pulley is installed at the lower end of the first rotating shaft and is connected to the synchronous belt drive structure.

4. The PCR plate sealing instrument according to claim 3, characterized in that: The synchronous belt drive structure includes: a second synchronous belt, a second rotating shaft, and a fourth synchronous pulley; The second rotating shaft is fixed to the upper end of the base plate, and the fourth synchronous pulley is mounted on the second rotating shaft and can rotate around the second rotating shaft; The third synchronous pulley is connected to the fourth synchronous pulley via the second synchronous belt; The second synchronization belt is connected to the sliding component.

5. The PCR plate sealing instrument according to claim 4, characterized in that: The sliding assembly includes: a first slider, a first linear guide rail, a timing belt connecting block, a pressure plate, a first zero-position contact plate, and a first sensor; The upper plate has a through groove running vertically through it; The groove has parallel first linear guide rails on both sides; the first sliders are respectively provided on the first linear guide rails; the stage is fixed on the first sliders. The timing belt connecting block passes through the groove and is fixedly connected to the bottom of the stage; The second synchronous belt is fixed to the synchronous belt connecting block by the pressure plate; The first zero-position contact plate is disposed on the stage, and the first sensor is disposed on the upper plate, with the first zero-position contact plate cooperating with the first sensor.

6. The PCR plate sealing instrument according to claim 1, characterized in that: The roller includes a shaft core; the shaft core has a cylindrical structure and is covered with polyurethane antistatic adhesive.

7. The PCR plate sealing instrument according to claim 3 or 4, characterized in that: The first synchronous pulley is a 15-tooth 3M synchronous pulley; the second, third, and fourth synchronous pulleys are 45-tooth 3M synchronous pulleys.

8. The PCR plate sealing instrument according to any one of claims 1-5, characterized in that: The platform is equipped with positioning pins that cooperate with the adapter.

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