Sampling Device and Sampling Method

Abstract

The present invention discloses a sampling device and a sampling method. The device includes a sampling needle, a main channel, a first switching element, a sampling driving member, and a pressure building assembly. The first switching element is disposed on the main channel. The first switching element divides the main channel into a first cavity and a second cavity. The first switching element is used to cut off or connect the first cavity and the second cavity. The first cavity is communicated with the sampling needle. Both the sampling driving member and the pressure building assembly are connected to the second cavity. Wherein, the pressure building assembly is used to establish a pressure that balances with the current ambient pressure at the needle opening of the sampling needle. When sampling is performed using the sampling device and the sampling method of the present invention, it can not only avoid reagent contamination of the blood collection tube during the sampling process, but also avoid air impurities from mixing into the collected blood sample.

Description

Technical Field

[0001] The present invention relates to medical devices, and particularly to a sampling device and a sampling method. Background Art

[0002] In medical devices, more and more instruments adopt automatic sampling. For example, a hematology analyzer punctures a vacuum blood collection tube through a sampling device with a sampling needle, and automatically aspirates a blood sample by establishing negative pressure on the sampling needle. However, during the collection process using the existing collection device, after the sampling needle punctures into the vacuum blood collection tube, the reagent at the needle tip is very likely to contaminate the sample in the vacuum blood collection tube. Moreover, after the sampling needle is withdrawn, the sample at the needle tip will mix with air impurities under the action of environmental pressure. These air impurities enter the reaction container along with the sampling needle, which is very likely to affect the test results. In addition, the pipeline used in the existing sampling device has a large deformation degree under negative pressure, which will affect the pipeline diameter and is likely to affect the sampling accuracy. Summary of the Invention

[0003] The main object of the present invention is to provide a sampling device and a sampling method to avoid reagent contamination of the blood collection tube during sampling and avoid air impurities from mixing into the collected blood sample.

[0004] To achieve the above object and other related objects, the technical solution of the present invention is as follows:

[0005] A sampling device includes a sampling needle, a main channel, a first switching element, a sampling driving member, and a pressure building component. The first switching element is disposed on the main channel. The first switching element divides the main channel into a first cavity and a second cavity. The first switching element is used to cut off or connect the first cavity and the second cavity. The first cavity is connected to the sampling needle. Both the sampling driving member and the pressure building component are connected to the second cavity. Wherein, the pressure building component is used to establish a pressure that balances the current environmental pressure at the needle tip of the sampling needle.

[0006] Optionally, the pressure building component includes a negative pressure source and a second switching element. The negative pressure source is used to establish a negative pressure that balances the pressure of the sampled container when the needle tip of the sampling needle is inside the sampled container. The second switching element is used to cut off or connect the second cavity and the negative pressure source.

[0007] Optionally, the pressure building component further includes a positive pressure source and a third switching element. The positive pressure source is used to establish a pressure that balances the external environment where the sampling device is located when the sampling needle is outside the sampled container. The third switching element is used to cut off or connect the second cavity and the positive pressure source;

[0008] Or

[0009] The pressure building component further includes an ambient pressure channel and a fourth switching element. The ambient pressure channel communicates the second cavity with the external environment where the sampling device is located, and the fourth switching element is used to cut off or connect the second cavity with the external environment where the sampling device is located.

[0010] Optionally, the sampling device further includes a cleaning component, which includes a cleaning swab, a liquid supply device, a liquid discharge device, a fifth switching element, and a sixth switching element. The cleaning swab is used to clean the outer wall of the sampling needle. The liquid supply device is connected to the cleaning swab, the liquid supply device is connected to the cleaning swab, the liquid discharge device is connected to the cleaning swab, the fifth switching element is used to cut off or connect the liquid supply device and the cleaning swab, and the sixth switching element is used to cut off or connect the liquid discharge device and the cleaning swab.

[0011] Optionally, the liquid supply device and the fifth switching element are communicated through a liquid supply channel. An internal cleaning channel is connected between the liquid supply channel and the second cavity. A seventh switching element is arranged on the internal cleaning channel, and the seventh switching element is used to cut off or connect the liquid supply device and the second cavity.

[0012] Optionally, the sampling driving member has two interfaces. The sampling driving member is on the main channel. The first interface is connected with a first hose, and the second interface is connected with a second hose, so that the first hose and the second hose form a section of the second cavity.

[0013] Among them, in the channels on the main channel excluding the channels of the first hose and the second hose, the channels through which the pressure building component is connected to the second cavity, and the channels corresponding to the first cavity, some or all of the channels are formed on rigid pipes and / or rigid elements.

[0014] Optionally, the rigid element includes a valve block.

[0015] The valve block is one piece. A first valve internal channel and a second valve internal channel are opened in the valve block. The first interface of the sampling driving member is connected to the first valve internal channel through the first hose, and the second interface of the sampling driving member is connected to the second valve internal channel through the second hose, so that the second cavity includes a partial section of the first valve internal channel, a partial section of the second valve internal channel, the channel in the first hose, and the channel in the second hose.

[0016] Or

[0017] The valve block is divided into two pieces, namely the first valve block and the second valve block. A first internal valve channel is provided in the first valve block, and a second internal valve channel is provided in the second valve block. The first interface of the sampling driving member is connected to the first internal valve channel through the first hose, and the second interface of the sampling driving member is connected to the second internal valve channel through the second hose, so that the second cavity includes a partial section of the first internal valve channel, a partial section of the second internal valve channel, the channels in the first hose, and the channels in the second hose.

[0018] Optionally, the first switching element is installed on the valve block and is located on the first internal valve channel. The sampling needle is communicated with the first internal valve channel through a first rigid pipe, so that the first cavity includes a partial section of the first internal valve channel and the channels in the first rigid pipe.

[0019] Optionally, when the sampling device includes a cleaning assembly, the cleaning assembly includes a liquid supply device, a liquid supply channel, a cleaning swab, an internal cleaning channel, and a seventh switching element. The cleaning swab is used to clean the outer wall of the sampling needle. The liquid supply device is connected to the cleaning swab through the liquid supply channel. The internal cleaning channel communicates the liquid supply channel and the second cavity. When the seventh switching element is provided on the internal cleaning channel,

[0020] The liquid supply channel is connected to the second internal valve channel of the valve block through a fourth rigid pipe. The internal cleaning channel includes a partial section of the second internal valve channel and the fourth rigid pipe.

[0021] Optionally, the pressure building assembly is connected to the second cavity through a first pressure building channel and a second pressure building channel. The pressure building assembly is connected to the valve block through a second rigid pipe and a third rigid pipe;

[0022] Wherein, the first pressure building channel includes the channels in the second rigid pipe and a partial section of the first internal valve channel or the first pressure building channel includes the channels in the second rigid pipe and a partial section of the second internal valve channel; the second pressure building channel includes the channels in the third rigid pipe and a partial section of the first internal valve channel or the second pressure building channel includes the channels in the third rigid pipe and a partial section of the second internal valve channel.

[0023] A sampling method is carried out by using any one of the above-mentioned sampling devices. The sampling method includes:

[0024] Open the first switching element to communicate the first cavity and the second cavity, and control the driving member to act to form an air column at the needle opening of the sampling needle;

[0025] Turn off the first switching element, and use the pressure - building component to pre - establish a first negative pressure in the second cavity for balancing the negative pressure in the vacuum blood collection tube.

[0026] Insert the sampling needle into the vacuum blood collection tube, and after the needle tip of the sampling needle is inserted below the liquid level, then turn on the first switching element.

[0027] Control the driving part to act again to collect a preset amount of blood samples.

[0028] After the driving part stops acting, turn off the first switching element, and use the pressure - building component to pre - establish a positive pressure for balancing the external environment where the sampling device is located.

[0029] Withdraw the sampling needle from the vacuum blood collection tube, and turn on the first switching element again.

[0030] Optionally, when using the pressure - building component to establish the first negative pressure, a stable negative - pressure source is adopted, and the pressure of the stable negative - pressure source is higher than the pressure of the vacuum blood collection tube.

[0031] When using the sampling device and sampling method of the present invention for sampling, it can not only avoid reagent contamination of the blood collection tube during the sampling process, but also avoid air impurities from mixing into the collected blood samples. Brief Description of the Drawings

[0032] Figure 1 Showing an exemplary liquid - path diagram of the sampling device of the present invention;

[0033] Figure 2 Showing another exemplary liquid - path diagram of the sampling device of the present invention;

[0034] Figure 3 Showing yet another exemplary liquid - path diagram of the sampling device of the present invention;

[0035] Figure 4 Showing still another exemplary liquid - path diagram of the sampling device of the present invention;

[0036] Figure 5 Showing another exemplary liquid - path diagram of the sampling device of the present invention;

[0037] Figure 6 Showing Figures 1 to 5 the liquid - path diagram of the main path connected to the sampling needle in

[0038] Figure 7 Showing Figure 1 or Figure 2 an exemplary schematic diagram of the valve block corresponding to the sampling device in

[0039] Figure 8 Showing Figure 1 or Figure 2Another exemplary schematic diagram of the valve block corresponding to the sampling device in

[0040] Figure 9 Shown as Figure 3 An exemplary schematic diagram of the valve block corresponding to the sampling device in

[0041] Figure 10 Shown as Figure 3 Another exemplary schematic diagram of the valve block corresponding to the sampling device in

[0042] Figure 11 Shown as Figure 4 An exemplary schematic diagram of the valve block corresponding to the sampling device in

[0043] Figure 12 Shown as Figure 4 Another exemplary schematic diagram of the valve block corresponding to the sampling device in

[0044] Figure 13 Shown as Figure 5 An exemplary schematic diagram of the valve block corresponding to the sampling device in

[0045] Figure 14 Shown as Figure 5 Another exemplary schematic diagram of the valve block corresponding to the sampling device in Detailed implementation manners

[0046] In the following description, numerous specific details are given to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details. In other instances, in order to avoid confusion with the present invention, some well-known technical features are not described.

[0047] It should be understood that the present invention can be implemented in different forms and should not be construed as limited to the embodiments presented herein. On the contrary, providing these embodiments will make the disclosure thorough and complete, and will fully convey the scope of the present invention to those skilled in the art. In the drawings, the same reference numerals denote the same components throughout.

[0048] The sampling devices in the following embodiments take the collection of blood samples in vacuum blood collection tubes as an example. In actual implementation, the collection device of the present invention can also be applied to collect other samples, and the volume of the liquid to be collected is not limited to vacuum tubes, but can also be other vacuum containers.

[0049] See Figures 1 to 6, the sampling device of the present invention includes a sampling needle 1, a main channel, a first switching element SV1, a sampling driving member 2, and a pressure building assembly (including 31 and 32). The first switching element SV1 is disposed on the main channel, and the first switching element SV1 divides the main channel into a first cavity 501 and a second cavity 502. The first switching element SV1 is used to cut off or communicate the first cavity 501 and the second cavity 502. The first cavity 501 is communicated with the sampling needle 1, and both the sampling driving member 2 and the pressure building assembly are connected to the second cavity 502. Among them, the pressure building assembly is used to establish a pressure that balances the current ambient pressure at the needle opening of the sampling needle 1.

[0050] Before sampling, the sampling needle 1 and the main channel are filled with a reagent. Here, the reagent is a cleaning solution. During sampling, the following steps are carried out:

[0051] 101. Open the first switching element SV1 to communicate the first cavity 501 and the second cavity 502, and use the sampling driving member 2 to form an air column at the needle opening of the sampling needle 1;

[0052] 103. Close the first switching element SV1 to block the first cavity 501 and the second cavity 502, and pre-establish a first negative pressure by the pressure building assembly to balance the negative pressure in the vacuum blood collection tube;

[0053] 105. Insert the sampling needle 1 into the vacuum blood collection tube. After the needle opening of the sampling needle is inserted below the liquid level, then open the first switching element SV1 so that the negative pressure established by the pressure building assembly can balance the negative pressure in the vacuum blood collection tube. Since there is a small air column in the sampling needle 1, the reagent will not contaminate the blood sample in the vacuum blood collection tube during the collection process, and the air content is small, and the influence on the detection accuracy can also be ignored. And this air column can separate the reagent and the collected blood sample, which can not only avoid the reagent from contaminating the blood sample in the vacuum blood collection tube, but also avoid the collected blood sample from being contaminated by the reagent in the channel;

[0054] 106. Control the driving member 2 to act to collect a preset amount of blood sample; in the actual implementation process, the driving member 2 can be a syringe;

[0055] 107. Before the sampling needle 1 exits the vacuum blood collection tube, close the first switching element SV1 again, and use the pressure building assembly to establish a positive pressure to balance the external environmental pressure where the sampling device is located, so that after the sampling needle 1 exits the vacuum blood collection tube, the collected blood sample can still remain in the sampling needle 1 under the action of the external environmental pressure, and air will be squeezed into the needle opening of the sampling needle 1;

[0056] 109. Withdraw the sampling needle 1 from the vacuum blood collection tube, and then open the first switching element SV1 to squeeze out the air at the needle opening. These airs will not enter the subsequent reaction container along with the sampling needle 1, which can avoid the problem of affecting the detection result due to the mixing of air.

[0057] In the actual implementation process, if the absolute value of the first negative pressure established in step 103 is less than the absolute value of the negative pressure of the vacuum blood collection tube, that is, the pressure in the vacuum blood collection tube is lower than the first negative pressure. When the needle opening of the sampling needle 1 is inserted into the vacuum blood collection tube, part of the air at the needle opening of the sampling needle 1 will enter the vacuum blood collection tube, causing the negative pressure of the vacuum blood collection tube to adjust itself to balance with the first negative pressure, and the final blood collection volume will be equal to the preset volume. On the contrary, if the absolute value of the first negative pressure established in step 103 is greater than or equal to the absolute value of the negative pressure of the vacuum blood collection tube, that is, the pressure in the vacuum blood collection tube is higher than or equal to the first negative pressure. When the needle opening of the sampling needle 1 is inserted into the vacuum blood collection tube, part of the air in the sampling needle 1 will still enter the vacuum blood collection tube, resulting in an increase in the pressure difference between the pressure in the vacuum blood collection tube and the first negative pressure, and the final blood collection volume will be greater than the preset volume.

[0058] Therefore, when using the pressure building component to establish the first negative pressure, a stable negative pressure source with a pressure higher than the pressure of the vacuum blood collection tube is adopted, which can ensure that the sampling volume is always equal to the preset volume.

[0059] In order to make the first negative pressure established by the stable negative pressure source higher than the pressure of the vacuum blood collection tube, in some embodiments, the preset negative pressure range of the stable negative pressure source is between -20 kPa and -60 kPa.

[0060] In some embodiments, referring to Figures 1 to 6 , the pressure building component includes a negative pressure source 31 and a second switching element SV2. The negative pressure source 31 is used to establish a negative pressure that balances with the pressure of the sampled container when the needle opening of the sampling needle 1 is in the sampled container, and the second switching element SV2 is used to cut off or connect the second cavity 502 and the negative pressure source 31. That is, the negative pressure source 31 is used to establish the first negative pressure in step 103. The second switching element SV2 is closed during steps 101, 107, and 109, and is only opened when the first negative pressure needs to be established, that is, during steps 103 and 105.

[0061] In some embodiments, referring to Figure 1 、 Figures 3 to 6 , the pressure building component further includes a positive pressure source 32 and a third switching element SV3. The positive pressure source 32 is used to establish a pressure that balances with the external environment where the sampling device is located when the sampling needle 1 is outside the sampled container, and the third switching element SV3 is used to cut off or connect the second cavity 502 and the positive pressure source 32. That is, the positive pressure source 32 is used to establish the positive pressure in step 107. In other embodiments, referring to Figure 2, in order to establish the positive pressure in step 107, the pressure - building component includes an ambient - pressure channel and a fourth switching element SV4. The ambient - pressure channel connects the second cavity 502 and the external environment where the sampling device is located, and the fourth switching element SV4 is used to cut off or connect the second cavity 502 and the external environment where the sampling device is located.

[0062] For the third switching element SV3 and the fourth switching element SV4, they are both closed during steps 101, 103, and 105, and are only opened when positive pressure needs to be established, that is, during steps 107 and 109.

[0063] In some embodiments, referring to Figures 1 to 6 , the sampling device of the present invention further includes a cleaning component. The cleaning component includes a cleaning swab 43, a liquid - supply device 41, a liquid - discharge device 42, a fifth switching element SV5, and a sixth switching element SV6. The cleaning swab 43 is used to clean the outer wall of the sampling needle 1. The liquid - supply device 41 is connected to the cleaning swab 43, and the liquid - discharge device 42 is connected to the cleaning swab 43. The fifth switching element SV5 is used to cut off or connect the liquid - supply device 41 and the cleaning swab 43, and the sixth switching element SV6 is used to cut off or connect the liquid - discharge device 42 and the cleaning swab 43.

[0064] When sampling with this sampling device, during sampling, both the fifth switching element SV5 and the sixth switching element SV6 are in the closed state. After sampling is completed, the outer wall of the sampling needle 1 can be automatically cleaned by opening the fifth switching element SV5 and the sixth switching element SV6.

[0065] In some embodiments, referring to Figures 1 to 6 , the liquid - supply device 41 and the fifth switching element SV5 are connected through a liquid - supply channel 401. An internal cleaning channel 402 is connected between the liquid - supply channel 401 and the second cavity 502. A seventh switching element SV7 is provided on the internal cleaning channel 402, and the seventh switching element SV7 is used to cut off or connect the liquid - supply device 41 and the second cavity 502.

[0066] During sampling, the seventh switching element SV7 is in the closed state. After sampling is completed, by opening the seventh switching element SV7 and the first switching element SV1, the cleaning liquid flows through the second cavity 502, the second cavity 502, and the inside of the sampling needle 1 to clean the internal channels of the sampling device.

[0067] In some embodiments, the sampling driver 2 has two interfaces, namely a first interface 201 and a second interface 202. The sampling driver 2 is located on the main channel. The first interface 201 is connected to the interface a of the valve block through a first hose, and the second interface 202 is connected to the interface d of the valve block through a second hose, such that the first hose and the second hose form a section of the second cavity 502; wherein, among the channels on the main channel excluding the channels of the first hose and the second hose, the channel through which the pressure - building component is connected to the second cavity 502, and the channel corresponding to the first cavity 501, part or all of the channels are formed on a rigid pipe and / or a rigid element.

[0068] It is usually difficult to connect the sampling driver 2 with rigid materials. Here, the first interface 201 and the second interface 202 can only be connected to hoses, while the remaining channels are formed by rigid pipes or rigid elements. In this way, during the sampling process, except for the first hose and the second hose, the inner diameters of the channels hardly change due to air pressure changes, minimizing the deformation of the channels to the greatest extent, which is beneficial to improving the sampling accuracy. Especially in steps 101, 103, and 105, it can resist the negative pressure established by the pressure - building component and the negative pressure in the vacuum tube, resulting in a very small deformation. In actual implementation, the rigid pipe and the rigid element here refer to parts with very small deformation under the action of a large gas pressure. For example, the rigid pipe needs to meet the requirement that the deformation is less than or equal to 0.3 μL / Kpa, and its deformation is much smaller than that of a conventional rubber hose.

[0069] When the rigid element is a valve block, a pipe joint needs to be correspondingly set for the rubber hose connected to the valve block. Actually, the pipe joint also belongs to the aforementioned rigid element.

[0070] Specifically, in some embodiments, in order to form the channels in the above - mentioned collection device, the number of valve blocks can be one or two.

[0071] When the number of valve blocks is one, with reference to Figure 7 、 Figure 9 、 Figure 11 、 Figure 13 , a first valve - internal channel A and a second valve - internal channel B are opened in the valve block. The first interface 201 of the sampling driver 2 is connected to the first valve - internal channel A through a first hose (not shown in the figure), and the second interface 202 of the sampling driver 2 is connected to the second valve - internal channel B through a second hose, such that the second cavity 502 includes a partial section of the first valve - internal channel A, a partial section of the second valve - internal channel B, the channel in the first hose, and the channel in the second hose.

[0072] When the number of valve blocks is two, with reference to Figure 8 、 Figure 10 、 Figure 12 、 Figure 14, the two valve blocks are the first valve block 100 and the second valve block 200 respectively. A first internal valve passage A is provided in the first valve block 100, and a second internal valve passage B is provided in the second valve block 200. The first interface 201 of the sampling drive member 2 is connected to the first internal valve passage A through the first hose, and the second interface 202 of the sampling drive member 2 is connected to the second internal valve passage B through the second hose, so that the second cavity 502 includes a partial section of the first internal valve passage A, a partial section of the second internal valve passage B, the passage in the first hose, and the passage in the second hose.

[0073] In some embodiments, with reference to Figures 1 to 5 , Figures 7 to 14 , the first switching element SV1 is installed on the valve block and is located on the first internal valve passage A. The sampling needle 1 is communicated with the first internal valve passage A through the first rigid pipe via the interface b, so that the first cavity 501 includes a partial section of the first internal valve passage A and the passage in the first rigid pipe.

[0074] In some embodiments, refer to Figures 1 to 5 , when the sampling device includes a cleaning assembly, the cleaning assembly includes a liquid supply device 41, a liquid supply passage 401, a cleaning swab 43, an internal cleaning passage 402, and a seventh switching element SV7. The cleaning swab 43 is used to clean the outer wall of the sampling needle 1. The liquid supply device 41 is connected to the cleaning swab 43 through the liquid supply passage 401. The internal cleaning passage 402 communicates the liquid supply passage 401 and the second cavity 502. When the seventh switching element SV7 is provided on the internal cleaning passage 402, that is, when the cleaning assembly in the figure is adopted:

[0075] Refer to Figures 7 to 14 , the seventh switching element SV7 is installed on the valve block and is located on the second internal valve passage B. The liquid supply passage 401 is connected to the interface f of the valve block through the fourth rigid pipe. The internal cleaning passage 402 includes a partial section of the second internal valve passage B and the fourth rigid pipe. In the actual implementation process, the seventh switching element SV7 can also be installed on the fourth rigid pipe.

[0076] In some embodiments, the pressure building assembly is connected to the second cavity 502 through the first pressure building passage 301 and the second pressure building passage 302. The pressure building assembly is connected to the valve block through the second rigid pipe and the third rigid pipe. Here, the first pressure building passage 301 is the passage connecting the negative pressure source 31 and the second cavity 502, and the second pressure building passage 302 is the ambient pressure passage.

[0077] When the pressure building assembly is connected to the second cavity 502 in the manner shown in Figure 1 , Figure 2 , with reference to Figure 7 ,Figure 8 The negative pressure source 31 is connected to the interface e of the valve block through a second rigid pipe. The first pressure building channel 301 includes the channel inside the second rigid pipe and a partial section of the second inner valve channel B. The second pressure building channel 302 includes the channel inside the third rigid pipe and a partial section of the first inner valve channel A. The third rigid pipe is connected to the interface c of the valve block through access.

[0078] When the pressure building assembly is connected to the second cavity 502 in the Figure 3 shown manner, with reference to Figure 9 、 Figure 10 ,the negative pressure source 31 is connected to the interface e of the valve block through a second rigid pipe. The first pressure building channel 302 includes the channel inside the second rigid pipe and a partial section of the first inner valve channel A. The second pressure building channel includes the channel inside the third rigid pipe and a partial section of the first inner valve channel A. The third rigid pipe is connected to the interface c of the valve block through access.

[0079] When the pressure building assembly is connected to the second cavity 502 in the Figure 4 shown manner, with reference to Figure 11 、 Figure 12 ,the negative pressure source 31 is connected to the interface e of the valve block through a second rigid pipe. The first pressure building channel includes the channel inside the second rigid pipe and a partial section of the second inner valve channel B. The second pressure building channel includes the channel inside the third rigid pipe and a partial section of the second inner valve channel B. The third rigid pipe is connected to the interface c of the valve block through access.

[0080] When the pressure building assembly is connected to the second cavity 502 in the Figure 5 shown manner, with reference to Figure 13 、 Figure 14 ,the negative pressure source 31 is connected to the interface e of the valve block through a second rigid pipe. The first pressure building channel includes the channel inside the second rigid pipe and a partial section of the second inner valve channel B. The second pressure building channel includes the channel inside the third rigid pipe and a partial section of the first inner valve channel A. The third rigid pipe is connected to the interface c of the valve block through access.

[0081] In the actual implementation process, the second switching element SV2, the third switching element SV3 (or the fourth switching element SV4) can be installed on the valve block or on the corresponding rigid pipe. Additionally, in the above embodiments, when a certain switching element is on the first / second inner valve channel, it means that when the switching element is in the closed state, the part of the switching element used to block the first / second inner valve channel realizes the blockage when the valve core extends into the corresponding inner valve channel. In the description of the present invention, unless otherwise clearly specified and limited, the first feature being "above" or "below" the second feature may include the first and second features being in direct contact, or may include the first and second features not being in direct contact but being in contact through other features between them.

[0082] In the description of the present invention, the singular forms "a", "an" and "the" are also intended to include the plural forms unless the context clearly dictates otherwise. It should also be understood that the terms "comprising" and / or "including", when used in this specification, specify the presence of the stated features, integers, steps, operations, components and / or parts, but do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts and / or groups.

[0083] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Any person familiar with this technology can modify or change the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by those with ordinary knowledge in the technical field without departing from the spirit and technical idea disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. A sampling device, characterized in that: It includes a sampling needle, a main channel, a first switching element, a sampling driving member, and a pressure building assembly. The first switching element is arranged on the main channel. The first switching element divides the main channel into a first cavity and a second cavity. The first switching element is used to cut off or connect the first cavity and the second cavity. The first cavity is connected to the sampling needle. Both the sampling driving member and the pressure building assembly are connected to the second cavity. Among them, the pressure building assembly is used to establish a pressure that balances the current environmental pressure at the needle opening of the sampling needle. The pressure building assembly includes a positive pressure source. The sampling driving member has two interfaces. The sampling driving member is located on the main channel. The first interface is connected to a first hose, and the second interface is connected to a second hose, so that the first hose and the second hose form a section of the second cavity. The pressure building assembly further includes a third switching element. The positive pressure source is used to establish a pressure that balances the external environment where the sampling device is located when the sampling needle is outside the sampled container. The third switching element is used to cut off or connect the second cavity and the positive pressure source. Among them, in the channels on the main channel excluding the first hose and the second hose, the channels where the pressure building assembly is connected to the second cavity, and the channels corresponding to the first cavity, part or all of the channels are formed on hard pipes and / or rigid elements. The positive pressure source is configured to extrude the air at the needle opening of the sampling needle after the sampling needle exits the vacuum blood collection tube.

2. The sampling device according to claim 1, wherein: The pressure building assembly includes a negative pressure source and a second switching element. The negative pressure source is used to establish a negative pressure that balances the pressure of the sampled container when the needle opening of the sampling needle is inside the sampled container. The second switching element is used to cut off or connect the second cavity and the negative pressure source.

3. The sampling device according to claim 1, wherein: It further includes a cleaning assembly. The cleaning assembly includes a cleaning swab, a liquid supply device, a liquid discharge device, a fifth switching element, and a sixth switching element. The cleaning swab is used to clean the outer wall of the sampling needle. The liquid supply device is connected to the cleaning swab. The liquid discharge device is connected to the cleaning swab. The fifth switching element is used to cut off or connect the liquid supply device and the cleaning swab. The sixth switching element is used to cut off or connect the liquid discharge device and the cleaning swab. The liquid supply device and the fifth switching element are connected through a liquid supply channel. An internal cleaning channel is connected between the liquid supply channel and the second cavity. A seventh switching element is arranged on the internal cleaning channel. The seventh switching element is used to cut off or connect the liquid supply device and the second cavity.

4. The sampling device according to claim 1, wherein: The rigid element includes a valve block. There is one valve block. A first valve internal channel and a second valve internal channel are opened in the valve block. The first interface of the sampling driving member is connected to the first valve internal channel through the first hose. The second interface of the sampling driving member is connected to the second valve internal channel through the second hose, so that the second cavity includes a part of the first valve internal channel, a part of the second valve internal channel, the channel in the first hose, and the channel in the second hose. Or The valve block is composed of two parts, namely the first valve block and the second valve block. A first inner valve channel is provided in the first valve block, and a second inner valve channel is provided in the second valve block. The first interface of the sampling driving member is connected to the first inner valve channel through the first hose, and the second interface of the sampling driving member is connected to the second inner valve channel through the second hose, so that the second cavity includes a partial section of the first inner valve channel, a partial section of the second inner valve channel, the channel in the first hose, and the channel in the second hose.

5. The sampling device according to claim 4, characterized in that: The first switching element is installed on the valve block and is located on the first inner valve channel. The sampling needle is communicated with the first inner valve channel through the first rigid pipe, so that the first cavity includes a partial section of the first inner valve channel and the channel in the first rigid pipe.

6. The sampling device according to claim 5, characterized in that: When the sampling device includes a cleaning assembly, the cleaning assembly includes a liquid supply device, a liquid supply channel, a cleaning swab, an internal cleaning channel, and a seventh switching element. The cleaning swab is used to clean the outer wall of the sampling needle. The liquid supply device is connected to the cleaning swab through the liquid supply channel. The internal cleaning channel communicates the liquid supply channel and the second cavity. When the seventh switching element is arranged on the internal cleaning channel, The liquid supply channel is connected to the second inner valve channel of the valve block through the fourth rigid pipe. The internal cleaning channel includes a partial section of the second inner valve channel and the fourth rigid pipe.

7. The sampling device according to claim 4, characterized in that: The pressure building assembly is connected to the second cavity through a first pressure building channel and a second pressure building channel. The pressure building assembly and the valve block are connected through a second rigid pipe and a third rigid pipe; Among them, the first pressure building channel includes the channel in the second rigid pipe and a partial section of the first inner valve channel or the first pressure building channel includes the channel in the second rigid pipe and a partial section of the second inner valve channel; the second pressure building channel includes the channel in the third rigid pipe and a partial section of the first inner valve channel or the second pressure building channel includes the channel in the third rigid pipe and a partial section of the second inner valve channel.

8. A sampling method, characterized in that, The sampling is carried out by using the sampling device according to any one of claims 1 to 7. The sampling method includes: Open the first switching element to connect the first cavity and the second cavity, and control the driving member to act to form an air column at the needle opening of the sampling needle; Close the first switching element, and use the pressure building assembly to pre-establish a first negative pressure for balancing the negative pressure in the vacuum blood collection tube in the second cavity; Insert the sampling needle into the vacuum blood collection tube, and after the needle opening of the sampling needle is inserted below the liquid level, then open the first switching element; Control the driving member to act again to collect a preset amount of blood sample; After the driving member stops acting, close the first switching element, and use the pressure building assembly to pre-establish a positive pressure for balancing the external environment where the sampling device is located; Withdraw the sampling needle from the vacuum blood collection tube, and open the first switching element again.

9. The sampling method according to claim 8, characterized in that: When establishing the first negative pressure by using the pressure building component, a stable negative pressure source is adopted, and the pressure of the stable negative pressure source is higher than the pressure of the vacuum blood collection tube.

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