A colorimetric-based detection kit and application thereof

The quantitative detection of Met protein dimers was achieved by combining a colorimetric detection kit with the colorimetric reaction of G quadrivalent DNAase and heme. This solves the problems of high cost and complexity in existing technologies, improves the sensitivity and accuracy of detection, and is suitable for rapid bedside diagnosis of circulating tumor cells.

CN114895033BActive Publication Date: 2026-07-10NANJING UNIV OF POSTS & TELECOMM
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING UNIV OF POSTS & TELECOMM
Filing Date
2022-05-24
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing methods for detecting circulating tumor cells are costly, complex to operate, and lack sufficient sensitivity and accuracy, making it difficult to achieve quantitative detection. In particular, the weak dimer signal of Met protein makes it difficult to meet practical detection needs.

Method used

A colorimetric assay kit is used to detect the quantitative state of Met protein dimers by using G quadrivalent DNAase to bind to heme. Met membrane proteins are anchored by the T1 and T2 recognition chains, triggering the hairpin self-assembly (CHA) of H1 and H2 to form G quadrivalent structures. This catalyzes the oxidation of ABTS to produce a colorimetric reaction.

Benefits of technology

It reduces detection costs and operational complexity, improves detection sensitivity and accuracy, and enables rapid bedside diagnosis of circulating tumor cells, possessing high accuracy and broad application prospects.

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Abstract

The application discloses a detection kit based on a colorimetric method and application thereof, and the detection kit comprises two membrane protein recognition chains T1 and T2, two DNA hairpins H1 and H2 required for forming G-quadruplex, hematin required for a color developing reaction, hydrogen peroxide (H2O2) and ABTS. The membrane protein recognition chains T1 and T2 can anchor membrane proteins, and adjacent hybridization occurs when the membrane proteins dimerize, thereby triggering catalytic hairpin self-assembly of H1 and H2 to form a large number of G-quadruplex structures; when H2O2 exists, G-quadruplex / hematin formed by combining G-quadruplex and hematin can catalyze oxidation of ABTS to generate a color developing reaction. The detection kit has the characteristics of high sensitivity, good specificity, low cost, low dependence on equipment and simple operation in the detection of cell membrane protein dimerization, and also has good application performance in cell signal pathway visualization and rapid diagnosis of tumor cells at a bedside.
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