PCR amplification vessel consumable

Abstract

The application provides a PCR amplification container consumable, which comprises an amplification card body, a silica gel ball and a medical film, the amplification card body comprises a cylindrical part and a flat part; a side hole is arranged at the end of the cylindrical part, a liquid adding hole is arranged at the top, and the side hole is communicated with the liquid adding hole; the flat part comprises an amplification cavity, and the amplification cavity is communicated with the side hole through a flow channel; the silica gel ball is placed in the side hole and is in interference fit with the side hole; and the medical film is arranged on the two sides of the flat part and is in sealed connection with the flat part. The amplification card body is composed of the cylindrical part and the flat part, the liquid adding hole, the flow channel and the amplification cavity are arranged on the amplification card body, the liquid adding hole is matched with a sample adding suction head, cross contamination can be avoided, and large flux detection can be achieved; the thin sheet type amplification cavity and the film are combined to form an amplification reaction cavity, which provides a good foundation for rapid temperature change in PCR amplification, is helpful to realize rapid and high flux PCR nucleic acid detection, is helpful to improve the degree of automation application, and is thus helpful to improve the detection efficiency.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medical devices, in particular to a PCR amplification container consumable. BACKGROUND

[0002] Virus detection such as nucleic acid detection requires full automation of the process, i.e. sample in and result out, while high-throughput detection in an epidemic situation and avoiding cross-contamination are highly required. At present, the amplification consumables used for nucleic acid detection at home and abroad basically adopt two forms: one is based on the 96-well plate, eight-pipe and the like used in traditional laboratories, which has great limitations on the heating and cooling mode of the reaction process, so that the heating and cooling speed is limited in a certain range. The other improvement such as capillary and flat consumables, although the heating and cooling speed is improved, but it does not provide the conditions for full-automatic application.

[0003] The existing Chinese patent with publication number CN210886074U discloses a PCR reaction consumable for automatic nucleic acid detection, relating to the field of biological experiment consumables, and the technical solution points are that it comprises a pipe body, the pipe body is provided with a pipe opening, and further comprises a pipe cover for plugging the pipe opening, the outer side wall of the pipe body is provided with a pressing part for fixing the pipe body when the pipe cover is opened; the pipe cover comprises a cover plate and a cover body, the cover plate is provided with a protruding part as a force point when the pipe cover is pulled out of the pipe body; when the pipe cover is covered on the pipe body, a gap is formed between the pressing part and the protruding part; the cover body is provided with a cavity along the length direction of the cover body, and the cover plate is provided with a light passing part corresponding to the position of the cavity for light entering the pipe body.

[0004] The inventor believes that the prior art cannot simultaneously achieve single sample, automatic, continuous rapid heating and cooling, meet the detection demand of high-throughput, and provide the conditions for full-automatic application, and it is necessary to provide a more reasonable PCR amplification container consumable. SUMMARY

[0005] In view of the defects in the prior art, the purpose of the present application is to provide a PCR amplification container consumable.

[0006] According to the PCR amplification container consumable provided by the present application, it comprises an amplification card body, a silica gel ball and a medical film, the amplification card body comprises a cylindrical part and a flat part; the cylindrical part is provided with a side hole at the end and a liquid adding hole at the top, and the side hole is communicated with the liquid adding hole; the flat part comprises an amplification cavity, and the amplification cavity is communicated with the side hole through a flow channel; the silica gel ball is placed in the side hole and the two are in interference fit; and the medical film is arranged on both sides of the flat part and is sealedly connected.

[0007] Preferably, the amplification cavity comprises a hollow circular amplification cavity, and the flow channel comprises a hollow micro flow channel.

[0008] Preferably, the cylindrical part is horizontally arranged, and the flat part is arranged directly below the cylindrical part.

[0009] Preferably, a positioning notch is arranged at the top center of the cylindrical part.

[0010] Preferably, the side hole is arranged at both ends of the cylindrical part, and the side hole comprises a cylindrical hole and is coaxially arranged with the cylindrical part.

[0011] Preferably, the silica gel ball is arranged in any of the side holes and is located between the outer end of the side hole and the liquid adding hole.

[0012] Preferably, the silica gel ball can move along the direction of the side hole, and when the silica gel ball moves to the position of the liquid adding hole, the silica gel ball blocks and seals the liquid adding hole and the inlet of the flow channel.

[0013] Preferably, the two liquid adding holes are symmetrically arranged at the top of the cylindrical part, and the two flow channels are symmetrically arranged at both sides of the amplification cavity, and the inlet of the flow channel is coaxially arranged with the liquid adding hole.

[0014] Preferably, the inlet of the flow channel is trumpet-shaped, and the inlet of the flow channel gradually increases from the flow channel to the liquid adding hole.

[0015] Preferably, the shape of the medical film matches the shape of the flat part, and the medical film is bonded or fused to the flat part.

[0016] Compared with the prior art, the present application has the following beneficial effects:

[0017] 1. The amplification card body composed of a cylindrical part and a flat part is provided with a liquid adding hole, a flow channel and an amplification cavity, the liquid adding hole matches the sample adding suction head, can meet the large flux detection and avoid cross contamination, the thin sheet type amplification cavity and the film combined form an amplification reaction cavity, which provides a good foundation for the rapid temperature change in PCR amplification, helps to realize rapid and high flux PCR nucleic acid detection, helps to improve the degree of automation application, and thus helps to improve the detection efficiency.

[0018] 2. The silica gel ball is in interference fit with the side hole, so that the silica gel ball can seal the side hole, and after the silica gel ball is pressed into the side hole, the liquid adding hole and the inlet of the flow channel can be sealed, which helps to realize the overall sealing, helps to improve the degree of automation application, and helps to improve the detection efficiency.

[0019] 3. The positioning notch arranged at the top of the cylindrical part helps to guide the movement of the amplification card on the applicable equipment and accurately position the amplification card, helps to improve the degree of automation application, helps to reduce the degree of manual participation, and thus helps to improve the detection efficiency. Attached Figure Description

[0020] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0021] Figure 1 This is a schematic diagram illustrating the overall structure of the PCR amplification container consumables, which is the main feature of this invention.

[0022] Figure 2 This is a front view of the PCR amplification container consumables that are the main features of this invention;

[0023] Figure 3 This is a left view of the PCR amplification container consumables that are the main features of this invention;

[0024] Figure 4 This is a right view of the PCR amplification container consumables that are the main features of this invention;

[0025] Figure 5 This is a top view of the PCR amplification container consumables that are the main features of this invention;

[0026] Figure 6 This is a bottom view of the PCR amplification container consumables, which is the main feature of this invention.

[0027] As shown in the figure:

[0028] Amplification card 1, Amplification chamber 11, Flow channel 12

[0029] Side hole 13, liquid filling hole 14, positioning notch 15

[0030] 2. Silicone balls; 3. Medical film Detailed Implementation

[0031] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all fall within the protection scope of the present invention.

[0032] like Figures 1-6 As shown, a PCR amplification container consumable provided by the present invention includes: an amplification card body 1, a silicone ball 2, and a medical film 3. The amplification card body 1 includes a cylindrical part and a flat part; the cylindrical part has a side hole 13 at its end and a liquid filling hole 14 at its top, and the side hole 13 communicates with the liquid filling hole 14; the flat part includes an amplification chamber 11, and the amplification chamber 11 communicates with the side hole 13 through a flow channel 12; the silicone ball 2 is placed in the side hole 13, and the two are press-fitted; the medical film 3 is disposed on both sides of the flat part, and the two are sealed together.

[0033] The application is suitable for nucleic acid detection, can meet the requirements of large throughput detection and avoid cross contamination, has fast temperature rising and falling speed in use, reduces the degree of manual participation in the detection process, and can effectively improve the detection efficiency.

[0034] The cylindrical part is horizontally arranged, and the flat part is arranged directly below the cylindrical part. The amplification card body 1 combined with the cylindrical part and the flat part is convenient for directional movement and positioning on the monitoring equipment, so as to realize positioning, transmission, centrifugation and automatic sealing in the full-automatic operation process.

[0035] Two liquid adding holes 14 are symmetrically arranged at the top of the cylindrical part, and two flow channels 12 are symmetrically arranged at the two sides of the amplification cavity 11. The inlet of the flow channel 12 is arranged on the same central axis as the liquid adding hole 14. When the reaction liquid is added, only one liquid adding hole 14 is used, and the other liquid adding hole 14 is used as an exhaust hole. The sample adding pipette of the reaction liquid is matched with the liquid adding hole 14, and the end of the silica gel ball 2 can seal the side hole 13, so as to cut off and block the liquid adding hole 14 along the side hole 13, so as to achieve the purpose of sealing the liquid adding hole 14 and the flow channel 12. The reaction liquid will not leak outward during the adding process, and will not cause pollution, effectively ensuring the accuracy of the detection.

[0036] The inlet of the flow channel 12 is in a trumpet shape, and the inlet of the flow channel 12 gradually increases from the flow channel 12 to the liquid adding hole 14. Generally, the size of the flow channel 12 is smaller than the size of the liquid adding hole 14, and a trumpet-shaped transition stage is needed to ensure that the reaction liquid enters the amplification cavity 11 smoothly.

[0037] The amplification cavity 11 can be arranged in various shapes, including ellipse, square, diamond, and matched with the flat part. Preferably, the amplification cavity 11 is a circular amplification cavity. The flow channel 12 can be arranged in various shapes. Preferably, the flow channel 12 is a sheet type micro flow channel, which can effectively control the flow rate, ensure the smooth flow of the reaction liquid, and improve the detection accuracy.

[0038] Further, the amplification cavity 11 can be hollow or hollowed out or grooved, and the flow channel 12 can also be hollow or hollowed out or grooved. According to the actual detection needs, some detections need to be heated or cooled, and the liquid in the hollowed-out flat part is separated from the outside by only a medical film 3 as a heat conducting medium, which can quickly heat or cool, thereby improving the test speed and realizing large-scale detection.

[0039] The shape of the medical film 3 matches the shape of the flat part, and the medical film 3 is bonded or fused with the flat part to achieve liquid sealing effect.

[0040] Further, the position between the two flow channels 12 on the flat part can be designed as hollow or hollowed out, which can effectively reduce the weight of the flat part and also reduce the production cost.

[0041] The top center of the cylindrical part is provided with a positioning gap 15. After the amplification card body 1 is placed on the detection equipment, the amplification card body 1 can move forward and backward along the axis of the cylindrical part. The positioning gap 15 is used for positioning during the axial movement, which greatly reduces the labor cost.

[0042] The two ends of the cylindrical part are provided with side holes 13. The side holes 13 include cylindrical holes and are coaxial with the cylindrical part. A silica gel ball 2 is placed in each side hole 13. The silica gel ball 2 is located between the outer end of the side hole 13 and the liquid adding hole (14). Preferably, the outer end of the side hole 13 and the outer end of the silica gel ball 2 placed therein are located in the same vertical plane.

[0043] The silica gel ball 2 includes an elastic ball capable of swelling. The diameter of the silica gel ball 2 is larger than the hole diameter of the side hole 13. In the initial position, the silica gel ball 2 is fixed at the end of the side hole 13, and the silica gel ball 2 is in a tight state and can seal the end of the side hole 13.

[0044] The silica gel ball 2 can move along the direction of the side hole 13. When the silica gel ball 2 moves to the position of the liquid adding hole 14, the silica gel ball 2 seals the liquid adding hole 14 and the inlet of the flow channel 12. After the amplification card body 1 is placed on the detection equipment, when the amplification card body 1 moves forward and backward along the axis of the cylindrical part, the silica gel ball 2 cooperates with the top rod on the detection equipment. The top rod presses the silica gel ball 2 into the inside of the side hole 13. Under the action of tension, the silica gel ball 2 expands outward to seal, thereby sealing the liquid adding hole 14 and the inlet of the flow channel 12.

[0045] When the application is used, the reaction liquid is added in one liquid adding hole 14. After centrifugation on the equipment, all the liquids participating in the reaction pass through the flow channel 12 into the amplification cavity 11. When the amplification card body 1 moves forward and backward along the axis of the cylindrical part, it cooperates with the top rod on the equipment. The top rod presses the silica gel ball 2 into the bottom of the side hole 13, thereby sealing the liquid adding hole 14 and the inlet of the flow channel 12, so as to seal the liquid in the amplification cavity 11.

[0046] The application can meet the automatic application, including automatic liquid adding, centrifugation, movement positioning, automatic gasket sealing, film sealing and heat conduction, which greatly improves the rapid temperature rise and fall during application. Moreover, the application can effectively reduce the degree of manual participation in the detection process and can meet the large flux detection, thereby effectively improving the detection efficiency.

[0047] Working principle

[0048] When the application is used, the reaction liquid is added in one liquid adding hole 14. After centrifugation on the equipment, all the liquids participating in the reaction pass through the flow channel 12 into the amplification cavity 11. When the amplification card body 1 moves forward and backward along the axis of the cylindrical part, it cooperates with the top rod on the equipment. The top rod presses the silica gel ball 2 into the bottom of the side hole 13, thereby sealing the liquid adding hole 14 and the inlet of the flow channel 12, so as to seal the liquid in the amplification cavity 11.

[0049] In the description of the present application, it needs to be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0050] The specific embodiments of the present application are described above. It needs to be understood that the present application is not limited to the above specific embodiments, and those skilled in the art can make various changes or modifications within the scope of the claims, which does not affect the essential content of the present application. The embodiments of the present application and the features in the embodiments can be arbitrarily combined with each other without conflict.

Claims

1. A PCR amplification vessel consumable, characterized by, The application relates to a nucleic acid amplification card, which comprises a nucleic acid amplification card body (1), silica gel balls (2) and medical film (3). The nucleic acid amplification card body (1) comprises a cylindrical part and a flat part. The cylindrical part is provided with side holes (13) at the ends and liquid adding holes (14) at the top. The side holes (13) are in communication with the liquid adding holes (14). The flat part comprises an amplification cavity (11) in communication with the side holes (13) through flow channels (12). The silica gel balls (2) are placed in the side holes (13) and are in interference fit with the side holes (13). The medical film (3) is arranged on both sides of the flat part and is in sealed connection with the flat part. The cylindrical part is provided with the side holes (13) at both ends.

2. The PCR amplification vessel consumable of claim 1, wherein, The side holes (13) comprise cylindrical holes and are coaxial with the cylindrical part.

3. The PCR amplification vessel consumable of claim 1, wherein, The silica gel balls (2) are placed in the side holes (13) and are located between the outer ends of the side holes (13) and the liquid adding holes (14).

4. The PCR amplification vessel consumable of claim 1, wherein, The amplification cavity (11) comprises a hollow circular amplification cavity.

5. The PCR amplification vessel consumable of claim 1, wherein, The flow channels (12) comprise hollow micro flow channels.

6. The PCR amplification vessel consumable of claim 1, wherein, The cylindrical part is horizontally arranged, and the flat part is arranged directly below the cylindrical part.

7. The PCR amplification vessel consumable of claim 1, wherein, The cylindrical part is provided with a positioning notch (15) at the top center.

8. The PCR amplification vessel consumable of claim 1, wherein, The silica gel balls (2) can move along the direction of the side holes (13). When the silica gel balls (2) move to the positions of the liquid adding holes (14), the silica gel balls (2) block and seal the liquid adding holes (14) and the entrances of the flow channels (12). The liquid adding holes (14) are symmetrically arranged on both sides of the top of the cylindrical part. The flow channels (12) are symmetrically arranged on both sides of the amplification cavity (11). The entrances of the flow channels (12) are coaxial with the liquid adding holes (14). The entrances of the flow channels (12) gradually increase from the flow channels (12) to the liquid adding holes (14). The medical film (3) is matched with the shape of the flat part. The medical film (3) is bonded or fused with the flat part.

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