A stirring method and mechanism for reaction liquid, and in vitro detection equipment

Abstract

The present invention discloses a stirring method and mechanism, and an in vitro detection device. Specifically, the present invention provides a stirring rod for stirring a reaction liquid; obtains detection methodological information of the reaction liquid; obtains stirring parameters based on the detection methodological information, wherein the stirring parameters at least include information on the movement trajectory of the stirring rod; and controls the stirring rod to stir the reaction liquid based on the stirring parameters. The present invention performs corresponding stirring on the reaction liquid based on the detection methodological information of the reaction liquid, has the advantages of being targeted and having a strong mixing ability, and improves the test speed and test accuracy.

Description

Technical Field

[0001] The present invention relates to the field of medical detection, and in particular to a stirring method and mechanism for a reaction liquid, and in vitro detection equipment. Background Art

[0002] In the field of automated medical device testing, achieving a uniform reaction solution and a stable reaction rate are crucial to the performance of the testing system. Therefore, a mechanism to assist in mixing the reaction solution is essential. The key to mixing is to increase the diffusion rate and relative contact area of the reagent in the sample, allowing the reagent and sample to form a uniform reaction solution in the shortest possible time, thereby creating a stable reaction rate for the testing process.

[0003] There are two main mixing methods available:

[0004] The first method uses high-speed vibration of the gripper to form a circular vortex in the cuvette. This mixing method works well for cuvettes with circular cross-sections, but is less effective for the rectangular cuvettes used in the dual-magnetic-circuit magnetic bead method. Furthermore, bubbles are easily formed during high-speed vibration, interfering with the test.

[0005] The second method is to use a syringe to aspirate and stir the reaction liquid to mix it. This mixing method uses the syringe's aspiration function to generate vertical movement of the liquid and the stirring function to drive the liquid's horizontal movement to achieve mixing. The disadvantages of this mixing method are large movement inertia and long mixing time, which affect the test speed. In addition, it requires support from the liquid system, which makes it easy to introduce contaminants. Summary of the Invention

[0006] The invention provides a reaction liquid stirring method and mechanism, and in vitro detection equipment.

[0007] According to a first aspect, the present invention provides a method for stirring a reaction liquid, comprising:

[0008] A stirring rod is provided for stirring the reaction liquid;

[0009] Obtaining detection methodology information of the reaction solution;

[0010] According to the detection methodology information, and taking into account the differences in detection methodology types, a stirring parameter including at least the stirring rod motion trajectory information is obtained;

[0011] controlling the stirring rod to stir the reaction liquid according to the stirring parameters;

[0012] When the detection methodology information is a first type of detection methodology, the stirring rod motion trajectory information is a two-dimensional motion of the stirring rod in a vertical plane, and the first type of detection methodology includes immunoturbidimetry and chromogenic substrate method; and / or,

[0013] When the detection methodology information is the second type of detection methodology, the stirring rod motion trajectory information is that the stirring rod stops at the original position and does not perform stirring. The second type of detection methodology includes a dual magnetic circuit magnetic bead method.

[0014] The present invention also provides another method for stirring the reaction solution, comprising:

[0015] A stirring rod is provided for stirring the reaction liquid;

[0016] Obtaining detection methodology information of the reaction solution;

[0017] obtaining stirring parameters including at least stirring rod motion trajectory information based on the detection methodology information and taking into account different detection methodology types; determining whether the reagent currently added to the reaction solution is a triggering reagent;

[0018] When it is determined that the reagent currently added to the reaction solution is a trigger reagent, controlling the stirring rod to stir the reaction solution according to the stirring parameter;

[0019] When the detection methodology information is a first type of detection methodology, the stirring rod motion trajectory information is a two-dimensional motion of the stirring rod in a vertical plane, and the first type of detection methodology includes immunoturbidimetry and chromogenic substrate method; and / or,

[0020] When the detection methodology information is the second type of detection methodology, the stirring rod motion trajectory information is that the stirring rod stops at the original position and does not perform stirring. The second type of detection methodology includes a dual magnetic circuit magnetic bead method.

[0021] The present invention also provides another method for stirring the reaction solution, comprising:

[0022] A stirring rod is provided for stirring the reaction liquid;

[0023] Obtaining detection methodology information of the reaction solution;

[0024] According to the detection methodology information, and taking into account the differences in detection methodology types, a stirring parameter including at least the stirring rod motion trajectory information is obtained;

[0025] According to the stirring parameters, the stirring rod is controlled to stir the reaction liquid.

[0026] The present invention also provides another method for stirring the reaction solution, comprising:

[0027] A stirring rod is provided for stirring the reaction liquid;

[0028] Obtaining detection methodology information of the reaction solution;

[0029] obtaining stirring parameters including at least stirring rod motion trajectory information based on the detection methodology information and taking into account different detection methodology types; determining whether the reagent currently added to the reaction solution is a triggering reagent;

[0030] When it is determined that the reagent currently added to the reaction liquid is a trigger reagent, the stirring rod is controlled to stir the reaction liquid according to the stirring parameter.

[0031] According to a second aspect, the present invention provides a stirring mechanism for a reaction liquid, comprising:

[0032] A stirring rod, used to stir the reaction solution;

[0033] A driving component, used for driving the stirring rod to move;

[0034] A processor is used to execute the stirring method described in any of the above embodiments.

[0035] According to a third aspect, the present invention provides an in vitro detection device comprising the above-mentioned stirring mechanism.

[0036] According to the stirring method and mechanism and in vitro detection equipment of the above embodiments, the reaction liquid is stirred accordingly according to the detection methodology information of the reaction liquid, which has the advantages of being targeted and having strong mixing ability, thereby improving the test speed and test accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 A flow chart of a stirring method according to an embodiment;

[0038] Figure 2 (a) and (b) are schematic diagrams of the motion trajectory of the stirring rod disclosed in the stirring method of an embodiment;

[0039] Figure 3 This is a schematic structural diagram of a container with a rectangular cross-section provided in one embodiment;

[0040] Figure 4 Schematic diagram of the structure of a stirring mechanism according to an embodiment;

[0041] Figure 5 A flow chart of a stirring method according to another embodiment;

[0042] Figure 6 A flowchart for determining whether a reagent currently added to a reaction solution is a triggering reagent in a stirring method according to an embodiment;

[0043] Figure 7 A schematic diagram of a running trajectory of a stirring rod performing one-dimensional motion in a horizontal plane in a stirring method according to an embodiment;

[0044] Figure 8A schematic structural diagram of a stirring mechanism according to another embodiment;

[0045] Figure 9 An illustration of stirring parameters according to an embodiment. DETAILED DESCRIPTION

[0046] The present invention will be further described in detail below by means of specific embodiments in conjunction with the accompanying drawings. Similar elements in different embodiments are numbered with associated similar elements. In the following embodiments, many detailed descriptions are provided to enable the present application to be better understood. However, those skilled in the art will readily appreciate that some of the features may be omitted in different circumstances, or may be replaced by other elements, materials, or methods. In some cases, some operations related to the present application are not shown or described in the specification. This is to avoid the core portion of the present application being overwhelmed by excessive descriptions, and for those skilled in the art, it is not necessary to describe these related operations in detail. They will fully understand the related operations based on the description in the specification and the general technical knowledge in the art.

[0047] In addition, the features, operations, or characteristics described in the specification may be combined in any appropriate manner to form various embodiments. Furthermore, the steps or actions in the method description may be reordered or adjusted in a manner readily apparent to those skilled in the art. Therefore, the various sequences in the specification and drawings are provided solely for the purpose of clearly describing a particular embodiment and are not intended to be mandatory, unless otherwise specified.

[0048] The serial numbers assigned to components herein, such as "first," "second," etc., are used solely to distinguish the objects being described and do not convey any sequential or technical meaning. References to "connection" and "coupling" herein, unless otherwise specified, include both direct and indirect connections (couplings).

[0049] As mentioned in the background art, the mixing methods using clamps or syringes each have their own shortcomings. Furthermore, the inventors have noted that different reaction solutions have varying requirements for mixing. For example, the activated partial thromboplastin time (APTT) uses ellagic acid as a mixed reagent to form a reaction solution with a sample, requiring only contact between the sample and reagent during mixing to facilitate diffusion. Furthermore, the inventors have discovered that different testing methodologies employed for the reaction solution may also result in different mixing requirements. For example, the measurement of D-dimer (DD) using latex immunoturbidimetry requires that the sample and latex be thoroughly mixed, but bubbles cannot be introduced. Therefore, for instruments that support multiple testing items and employ multiple testing methodologies, a single mixing method becomes a significant factor restricting the performance of the instrument.

[0050] In summary, the inventors not only took into account the shortcomings of the existing single clamp or syringe for mixing, but also took into account the differences in mixing requirements for different reaction liquids - typically different reagents added - and the differences in mixing requirements caused by different detection methodologies used for the reaction liquids. Therefore, a stirring method for the reaction liquid (hereinafter referred to as the stirring method) is proposed. The stirring method uses a stirring rod to stir the reaction liquid to achieve the purpose of mixing, which is described in detail below.

[0051] Example 1

[0052] Please refer to Figure 1 , a stirring method provided in one embodiment includes steps S110 to S140.

[0053] Step S110: Provide a stirring rod for stirring the reaction liquid. In one embodiment, the stirring rod is a round rod made of austenitic stainless steel, the top of which may be rounded. The top of the stirring rod refers to the end of the stirring rod extending into the reaction liquid.

[0054] Step S120: Acquire the detection methodology information of the reaction solution. In one embodiment, the detection methodology information may include the first type of detection methodology and the second type of detection methodology, etc. The first type of detection methodologies can include immunoturbidimetry Method and chromogenic substrate method, etc. , The second type of detection methodology can include dual magnetic circuit magnetic bead method, etc. .

[0055] Step S130: Based on the acquired detection methodology information, the stirring parameters are obtained, wherein the stirring parameters include at least the stirring rod motion trajectory information. In one embodiment, step S130 includes: the stirring rod motion trajectory information is the two-dimensional motion of the stirring rod in a vertical plane. In a specific embodiment, the stirring rod performs a two-dimensional motion in a vertical plane, including: the stirring rod repeatedly performs motion on a closed trajectory, and the closed trajectory is composed of a number of curve paths and / or line segment paths in the same vertical plane. As described above, the stirring parameters include at least the stirring rod motion trajectory information. In one embodiment, the stirring parameters also include at least one of the initial position of the top of the stirring rod, the speed of the stirring rod movement, and the time of the stirring rod movement. The above stirring parameters can be preset or set by the user through a relevant interactive interface.

[0056] The relationship between the detection methodology information and the stirring parameters in step S130 is described in detail below.

[0057] In one embodiment, in step 130, when the detection methodology information is the first type of detection methodology, the stirring rod motion trajectory information is that the stirring rod performs two-dimensional motion in a vertical plane. It should be noted that the initial position of the top end of the stirring rod can be any point on the closed trajectory, and the movement direction of the stirring rod can be always clockwise or always counterclockwise, or it can be alternating between clockwise and counterclockwise; and / or, when the detection methodology information is the second type of detection methodology, the stirring rod motion trajectory information is that the stirring rod stops at the original position and does not perform stirring.

[0058] As described above, when the detection methodology is the first type of detection methodology, the stirring rod motion trajectory information is the two-dimensional motion of the stirring rod in the vertical plane, for example, the stirring rod repeatedly performs motion on a closed trajectory, and the closed trajectory is composed of several curve paths and / or line segment paths in the same vertical plane.

[0059] In a specific embodiment, when the detection methodology information is a chromogenic substrate method, the obtained closed trajectory is composed of four line segment paths in the same vertical plane, wherein the four line segment paths are respectively a horizontal line segment path, two vertical line segment paths connected to both ends of the horizontal line segment path, and a line segment path connecting the two vertical line segments with the ends that are not connected to the horizontal line segment. For example, Figure 2 (a) shows an example of a closed trajectory when the detection methodology is a chromogenic substrate method. In the actual stirring process, the initial position of the stirring rod tip can be Figure 2 For any point in the closed trajectory in (a), let's take the initial position of the top of the stirring rod at point a in the figure and describe it as an example: the stirring rod starts from the position of its top at point a in the figure, first moves a distance in the upward direction, then moves a distance in the vertical direction, then moves back in the horizontal direction to just above point a, and then moves back in the vertical direction to point a, completing the movement of a closed trajectory. It can be understood that the initial position of the top of the stirring rod can be Figure 2 Any point in (a), and its direction of movement can also be clockwise along the closed trajectory.

[0060] In a specific embodiment, when the detection methodology information is immunoturbidimetry, the obtained closed trajectory is composed of two curved paths in the same vertical plane, wherein one curved path is a concave curve path, and the other curved path is a convex curve path, wherein one end of the concave curve path is connected to one end of the convex curve path, and the other end of the concave curve path is connected to the other end of the convex curve path. For example, Figure 2(b) shows an example of a closed trajectory when the detection methodology is immunoturbidimetry. In the actual stirring process, the initial position of the top of the stirring rod can be Figure 2 For any point in the closed trajectory in (b), let's take the initial position of the top of the stirring rod at point b in the figure and describe it as moving counterclockwise along the closed trajectory: starting from point b, it first moves diagonally downward along the convex curve for a distance, and then moves diagonally upward along the concave curve to return to point b, completing the movement of a closed trajectory. It can be understood that the initial position of the top of the stirring rod can be Figure 2 Any point in (b), and its direction of movement can also be clockwise along the closed trajectory.

[0061] The inventors have found that due to the movement of the stirring rod in the reaction liquid, especially when it moves at high speed, eddy currents will be generated near the top of the stirring rod. When performing two-dimensional movement in the vertical plane, the movement distance of the top of the stirring rod is relatively long. Therefore, when performing two-dimensional movement in the vertical plane, eddy currents can be generated over the longest possible distance. Figure 2 Comparison of the two-dimensional motion trajectories of the vertical plane (a) and (b). Figure 2 (a) has higher stirring efficiency, while Figure 2 (b) Stirring Fewer bubbles are generated during the process , therefore Figure 2 The stirring rod motion trajectory shown in (a) is suitable for the use of a device that is insensitive to bubbles but Stirring of reaction solutions for time-critical detection methodologies, such as the chromogenic substrate method described above, is not recommended. Figure 2 (b) is suitable for Stirring of the reaction solution using a detection method that is sensitive to bubbles, such as the above-mentioned immunoturbidimetric method .

[0062] Step S140: controlling the stirring rod to stir the reaction liquid according to the above stirring parameters.

[0063] The present invention provides a stirring rod for stirring. In one embodiment, the stirring method may further include providing a container with a non-circular cross section for containing the reaction liquid to cooperate with the stirring of the stirring rod. For example, please refer to Figure 3 , the present invention provides a container with a rectangular cross-section. Compared with a container with a circular cross-section, the container with a non-circular cross-section of the present invention, such as a container with a rectangular cross-section, has many advantages when combined with the stirring rod of the present invention. For example, for the dual-magnetic circuit magnetic bead method, it is more suitable to use a reaction vessel with a rectangular cross-section. The magnetic bead method is to arrange electromagnets around the reaction vessel, and generate magnetism by controlling the conduction of the electromagnets to drive the magnetic beads to swing. Using a rectangular cross-section reaction vessel, only two electromagnets can be arranged to effectively control the swing of the magnetic beads. The main advantage of the dual-magnetic circuit magnetic bead method over the optical method is anti-interference, because chyle, jaundice, bilirubin, etc. will cause the optical properties of the reaction solution to change, affecting the test results, but these substances will not affect the swing of the magnetic beads, and therefore will not interfere with the test of the magnetic bead method. For immunoturbidimetric and chromogenic substrate methods, the rectangular reaction vessel will not focus the light beam and will not affect the light path. It is simpler in light path arrangement and can use one less lens than a circular reaction vessel.

[0064] The above is the stirring method of one embodiment of the present invention. It performs corresponding stirring on the reaction liquid according to the detection methodology information of the reaction liquid. It is targeted and has strong mixing ability, thereby improving the test speed and test accuracy.

[0065] This embodiment also discloses an in vitro detection device, which can be used for quantitative and / or qualitative analysis and measurement of samples. In one embodiment, the in vitro detection device can include a stirring mechanism for the reaction liquid (hereinafter referred to as the stirring mechanism). Figure 4 The stirring mechanism includes a stirring rod 110, a driving component 120 and a processor 130, which are described in detail below.

[0066] The stirring rod 110 is used to stir the reaction liquid. In one embodiment, the stirring rod 110 is a round rod made of austenitic stainless steel, and the top end of the stirring rod 110 may be chamfered.

[0067] The driving component 120 is used to drive the stirring rod 110 to move. For example, in a three-dimensional rectangular coordinate system XYZ, the driving component 120 can drive the stirring rod to achieve two-dimensional movement in the horizontal X direction and the vertical Z direction. In one embodiment, the driving component 120 can be implemented using a stepper motor.

[0068] The processor 130 is used to obtain detection methodology information of the reaction liquid, and obtain stirring parameters based on the detection methodology information, where the stirring parameters at least include stirring rod motion trajectory information; and according to the above stirring parameters, the driving component 120 is controlled to control the stirring rod 110 to stir the reaction liquid.

[0069] In one embodiment, the stirring rod motion trajectory information obtained by the processor 130 is the two-dimensional motion of the stirring rod in a vertical plane. In a specific embodiment, the stirring rod performs two-dimensional motion in a vertical plane, including: the stirring rod repeatedly performs motion on a closed trajectory, and the closed trajectory is composed of a number of curved paths and / or line segment paths in the same vertical plane; in one embodiment, the stirring parameters obtained by the processor 130 may also include at least one of the initial position of the top of the stirring rod, the speed of the stirring rod movement, and the time of the stirring rod movement. The above stirring parameters can be preset or set by the user through a relevant interactive interface.

[0070] In one embodiment, when the detection methodology information of the processor 130 is the first type of detection methodology, the motion trajectory information of the stirring rod is the two-dimensional motion of the stirring rod in the vertical plane. It should be noted that the initial position of the top end of the stirring rod can be any point on the closed trajectory, and the movement direction of the stirring rod can be always clockwise or always counterclockwise, or it can be alternating between clockwise and counterclockwise; and / or, when the detection methodology information is the second type of detection methodology, the motion trajectory information of the stirring rod is that the stirring rod stops at the original position and does not perform stirring. Specifically, in one embodiment, when the detection methodology information of the processor 130 is the chromogenic substrate method, the closed trajectory obtained is composed of four line segment paths in the same vertical plane, wherein the four line segment paths are respectively a horizontal line segment path, two vertical line segment paths connected to the two ends of the horizontal line segment path, and a line segment path connecting the two vertical line segments to the end that is not connected to the horizontal line segment. For example, as mentioned above Figure 2 (a) shows an example of a closed trajectory when the detection methodology is a chromogenic substrate method. In the actual stirring process, the initial position of the stirring rod tip can be Figure 2 For any point in the closed trajectory in (a), let's take the initial position of the top of the stirring rod at point a in the figure and describe it as an example: the stirring rod starts from the position of its top at point a in the figure, first moves a distance in the upward direction, then moves a distance in the vertical direction, then moves back in the horizontal direction to just above point a, and then moves back in the vertical direction to point a, completing the movement of a closed trajectory. It can be understood that the initial position of the top of the stirring rod can be Figure 2 (a), and its movement direction can also be clockwise along the closed trajectory. In one embodiment, when the detection methodology information of the processor 130 is immunoturbidimetric method, the closed trajectory obtained is composed of two curved paths in the same vertical plane, one of which is a concave curve path and the other is a convex curve path. One end of the concave curve path is connected to one end of the convex curve path, and the other end of the concave curve path is connected to the other end of the convex curve path. For example, as mentioned above Figure 2 (b) shows an example of a closed trajectory when the detection methodology is immunoturbidimetry. In the actual stirring process, the initial position of the top of the stirring rod can be Figure 2For any point in the closed trajectory in (b), let's take the initial position of the top of the stirring rod at point b in the figure and describe it as moving counterclockwise along the closed trajectory: starting from point b, it first moves diagonally downward along the convex curve for a distance, and then moves diagonally upward along the concave curve to return to point b, completing the movement of a closed trajectory. It can be understood that the initial position of the top of the stirring rod can be Figure 2 Any point in (b), and its direction of movement can also be clockwise along the closed trajectory.

[0071] In order to cooperate with the stirring of the stirring rod 110, in one embodiment, the stirring mechanism further includes a container with a non-circular cross section for containing the reaction liquid, for example, please refer to the previous Figure 3 , the stirring mechanism may comprise a container of rectangular cross-section.

[0072] Example 2

[0073] Embodiment 1 is to perform corresponding stirring on the reaction liquid according to the detection methodology information of the reaction liquid. Sometimes, when a sample is to be subjected to a certain test item, one or more reagents may be added in sequence. After each reagent is added to the sample to form a reaction liquid, it may take some time for the reaction liquid to be naturally mixed, or it may be stirred to accelerate its mixing, and then the next reagent is added; generally, the last reagent added is defined as a trigger reagent, and the reagents added before are mixed reagents. Therefore, when the test item information of the reaction liquid is known, the reagents to be added to the reaction liquid or the initial sample and the order between them can be determined, so that it can be known which reagent is the last reagent to be added, that is, which reagent is the trigger reagent. This embodiment 2 combines the detection methodology information of the reaction liquid and the type of reagent currently added - a trigger reagent or a mixed reagent - to stir the reaction liquid, which is described in detail below.

[0074] Please refer to Figure 5 , an embodiment provides a stirring method, which includes steps S210 to S250.

[0075] Step S210: providing a stirring rod, wherein the stirring rod is used to stir the reaction liquid.

[0076] Step S220: Acquire detection methodology information of the reaction solution.

[0077] Step S230: obtaining stirring parameters according to the acquired detection methodology information, wherein the stirring parameters at least include stirring rod motion trajectory information.

[0078] The stirring method of this embodiment is the same as or similar to the stirring method of Example 1 in that the first several steps are the same or similar, for example, step S210 is the same or similar to step S110, step S220 is the same or similar to step S120, and step S220 is the same or similar to step S120. Therefore, these steps will not be described in detail. For details, please refer to the relevant description in Example 1.

[0079] Step S240: Determine whether the reagent currently added to the reaction solution is a trigger reagent. In one embodiment, please refer to Figure 6 , step S240 may include step S241 and step S243.

[0080] Step S241: Acquire test item information of the reaction solution.

[0081] Step S243: Based on the test item information, determine whether the reagent currently added to the reaction solution is a triggering reagent. For example, once the test item information for the reaction solution is known, the reagents to be added to the reaction solution, or the initial sample, and their order can be determined, thereby determining which reagent is the last reagent to be added, i.e., which reagent is the triggering reagent.

[0082] It should be noted that steps S220 and S230 obtain the stirring parameters by acquiring the detection methodology information of the reaction liquid. They can be located before or after step S240. For example, when the judgment result of step S240 is that the reagent currently added to the reaction liquid is a trigger reagent, steps S220 and S230 are performed.

[0083] Step S250: When it is determined that the reagent currently added to the reaction liquid is a trigger reagent, the stirring rod is controlled to stir the reaction liquid according to the above stirring parameters. For example, when the detection methodology information is the first type of detection methodology, then when it is determined that the reagent currently added to the reaction liquid is a trigger reagent, the stirring rod is controlled to perform two-dimensional movement in the vertical plane. In one embodiment, the stirring method may also include when it is determined that the reagent currently added to the reaction liquid is a mixed reagent, the stirring rod may be controlled not to stir the reaction liquid, or the stirring rod may be controlled to perform one-dimensional movement in the horizontal plane on the reaction liquid, that is, at this time the stirring rod operation trajectory information is that the stirring rod performs one-dimensional movement in the horizontal plane, and the stirring speed of the one-dimensional movement in the horizontal plane may be medium speed or low speed, Figure 7 The figure shows the running trajectory information of the stirring rod performing one-dimensional motion in the horizontal plane.

[0084] As mentioned above, some detection methodologies are not sensitive to bubbles generated during the stirring process but have strict requirements on the stirring time, while some detection methodologies are more sensitive to bubbles and the requirements on the stirring time can be appropriately relaxed. The requirements of these detection methodologies for the stirring process are generally reflected when the trigger reagent is finally added to the reaction liquid. When the mixing reagent is added to the reaction liquid before, there are generally no such requirements. For example, only mixing is required. Therefore, when it is determined that the trigger reagent is currently being added, the reaction liquid can be stirred according to the stirring parameters obtained in step S230. When it is determined that the mixing reagent is currently being added, the reaction liquid can be allowed to mix naturally without stirring, or simply perform one-dimensional motion in the horizontal plane to accelerate the mixing to save time. Of course, the stirring rod can also be controlled to stir according to the stirring parameters obtained in step S230.

[0085] In one embodiment, the stirring method may further include providing a container with a non-circular cross-section, wherein the container is used to contain the reaction liquid to cooperate with the stirring of the stirring rod.

[0086] The above is the stirring method of one embodiment of the present invention, which performs corresponding stirring on the reaction liquid according to the detection methodology information of the reaction liquid and whether the currently added reagent is a trigger reagent. It is targeted and has strong mixing ability, thereby improving the test speed and test accuracy.

[0087] This embodiment also discloses an in vitro detection device, which can be used for quantitative and / or qualitative analysis and measurement of samples. In one embodiment, the in vitro detection device can include a stirring mechanism for the reaction liquid (hereinafter referred to as the stirring mechanism). Figure 8 The stirring mechanism includes a stirring rod 210, a driving component 220 and a processor 230, which are described in detail below.

[0088] The stirring rod 210 is used to stir the reaction liquid. In one embodiment, the stirring rod 210 is a round rod made of austenitic stainless steel, and the top end of the stirring rod 210 may be chamfered.

[0089] The driving component 220 is used to drive the stirring rod 210 to move. For example, in a three-dimensional rectangular coordinate system XYZ, the driving component 220 can drive the stirring rod to achieve two-dimensional movement in the horizontal X direction and the vertical Z direction. In one embodiment, the driving component 220 can be implemented using a stepping motor.

[0090] The processor 230 is used to obtain detection methodology information of the reaction liquid, and obtain stirring parameters based on the obtained detection methodology information, where the stirring parameters at least include the movement trajectory information of the stirring rod; determine whether the reagent currently added to the reaction liquid is a trigger reagent. When it is determined that the reagent currently added to the reaction liquid is a trigger reagent, according to the above-mentioned stirring parameters, the stirring rod 210 is controlled by controlling the driving component 220 to stir the reaction liquid.

[0091] In one embodiment, the stirring rod motion trajectory information obtained by the processor 230 is the stirring rod performing two-dimensional motion in a vertical plane. In a specific embodiment, the stirring rod performing two-dimensional motion in a vertical plane includes: the stirring rod repeatedly performing motion on a closed trajectory, wherein the closed trajectory is composed of multiple curved paths and / or line segments in the same vertical plane. In one embodiment, the stirring parameters obtained by the processor 230 may also include at least one of the initial position of the stirring rod tip, the speed of the stirring rod movement, and the stirring rod movement time. The above stirring parameters may be preset or set by the user through a related interactive interface. In one embodiment, when the detection methodology information is the first type of detection methodology, the stirring rod motion trajectory information is the stirring rod performing two-dimensional motion in a vertical plane. It should be noted that the initial position of the stirring rod tip can be any point on the closed trajectory, and the stirring rod movement direction can be always clockwise, always counterclockwise, or alternately clockwise and counterclockwise. And / or, when the detection methodology information is the second type of detection methodology, the stirring rod motion trajectory information is the stirring rod remaining at its original position and not performing stirring. Specifically, in one embodiment, when the detection methodology information is a chromogenic substrate method, the processor 230 obtains a closed trajectory consisting of four line segment paths in the same vertical plane, wherein the four line segment paths are a horizontal line segment path, two vertical line segment paths connected to both ends of the horizontal line segment path, and a line segment path connecting the two vertical line segments to the ends that are not connected to the horizontal line segment. For example, as mentioned above Figure 2 (a) shows an example of a closed trajectory when the detection methodology is a chromogenic substrate method. In the actual stirring process, the initial position of the stirring rod tip can be Figure 2 For any point in the closed trajectory in (a), let's take the initial position of the top of the stirring rod at point a in the figure and describe it as an example: the stirring rod starts from the position of its top at point a in the figure, first moves a distance in the upward direction, then moves a distance in the vertical direction, then moves back in the horizontal direction to just above point a, and then moves back in the vertical direction to point a, completing the movement of a closed trajectory. It can be understood that the initial position of the top of the stirring rod can be Figure 2(a), and its movement direction can also be clockwise along the closed trajectory. In one embodiment, when the detection methodology information of the processor 230 is immunoturbidimetric method, the closed trajectory obtained is composed of two curved paths in the same vertical plane, one of which is a concave curve path and the other is a convex curve path. One end of the concave curve path is connected to one end of the convex curve path, and the other end of the concave curve path is connected to the other end of the convex curve path. For example, as mentioned above Figure 2 (b) shows an example of a closed trajectory when the detection methodology is immunoturbidimetry. In the actual stirring process, the initial position of the top of the stirring rod can be Figure 2 For any point in the closed trajectory in (b), let's take the initial position of the top of the stirring rod at point b in the figure and describe it as moving counterclockwise along the closed trajectory: starting from point b, it first moves diagonally downward along the convex curve for a distance, and then moves diagonally upward along the concave curve to return to point b, completing the movement of a closed trajectory. It can be understood that the initial position of the top of the stirring rod can be Figure 2 (b), and its movement direction can also be clockwise along the closed trajectory. In one embodiment, when the processor 230 determines that the reagent currently added to the reaction solution is a mixed reagent, it can control the stirring rod to not stir the reaction solution, or control the stirring rod to perform a one-dimensional motion in the horizontal plane on the reaction solution.

[0092] In order to cooperate with the stirring of the stirring rod 110, in one embodiment, the stirring mechanism further includes a container with a non-circular cross section for containing the reaction liquid, for example, please refer to the previous Figure 3 , the stirring mechanism may comprise a container of rectangular cross-section.

[0093] Please refer to Figure 9 , is a practical example of stirring parameters. Generally, the stirring speed of the one-dimensional motion in the horizontal plane mentioned above can be medium or low speed; the stirring speed of the two-dimensional motion in the vertical plane mentioned above can all be high speed. Figure 9 Method a corresponds to Figure 7 One-dimensional motion in the horizontal plane, Figure 9 Methods b and c correspond to, Figure 2 (a), (b); In addition, Figure 9 The unit "step" in the table refers to the length unit when the stirring rod is driven by a stepper motor.

[0094] It should be noted that the stirring method of the reaction liquid of the present application can be the stirring method of the reaction liquid of a coagulometer, the stirring mechanism of the present application can be the stirring mechanism of a coagulometer, and the in vitro detection equipment of the present application can also be a coagulometer.

[0095] Those skilled in the art will appreciate that all or part of the functions of the various methods in the above embodiments can be implemented by hardware or by computer program. When all or part of the functions in the above embodiments are implemented by computer program, the program can be stored in a computer-readable storage medium, and the storage medium can include: read-only memory, random access memory, disk, optical disk, hard disk, etc., and the program is executed by a computer to implement the above functions. For example, the program is stored in the memory of the device, and when the program in the memory is executed by the processor, all or part of the above functions can be implemented. In addition, when all or part of the functions in the above embodiments are implemented by computer program, the program can also be stored in a storage medium such as a server, another computer, disk, optical disk, flash disk or mobile hard disk, and saved in the memory of the local device by downloading or copying, or the system of the local device is updated. When the program in the memory is executed by the processor, all or part of the functions in the above embodiments can be implemented.

[0096] The above examples are used to illustrate the present invention, which are only used to help understand the present invention and are not intended to limit the present invention. Those skilled in the art can make several simple deductions, modifications or substitutions based on the concept of the present invention.

Claims

1. A method for stirring a reaction liquid, applied to an in vitro detection device, wherein the in vitro detection device comprises a stirring rod; characterized in that: include: Providing the stirring rod for stirring the reaction liquid; Obtaining detection methodology information of the reaction solution; According to the detection methodology information, and taking into account the differences in detection methodology types, a stirring parameter including at least the stirring rod motion trajectory information is obtained; the stirring parameter is preset or set through an interactive interface; controlling the stirring rod to stir the reaction liquid according to the stirring parameters; When the detection methodology information is a first-category detection methodology, the stirring rod motion trajectory information is a two-dimensional motion of the stirring rod in a vertical plane, and the two-dimensional motion direction is clockwise, counterclockwise, or alternating clockwise and counterclockwise. The first-category detection methodology includes immunoturbidimetry and chromogenic substrate method; wherein: When the detection methodology information is a chromogenic substrate method, the obtained closed trajectory is composed of four line segment paths in the same vertical plane, wherein the four line segment paths are respectively a horizontal line segment path, two vertical line segment paths connected to both ends of the horizontal line segment path, and a line segment path connecting the two vertical line segments with the ends that are not connected to the horizontal line segment; When the detection methodology information is immunoturbidimetry, the obtained closed trajectory is composed of two curved paths in the same vertical plane, one of which is a concave curve path and the other is a convex curve path. One end of the concave curve path is connected to one end of the convex curve path, and the other end of the concave curve path is connected to the other end of the convex curve path.

2. A method for stirring a reaction liquid, applied to an in vitro detection device, wherein the in vitro detection device comprises a stirring rod; characterized in that: include: Providing the stirring rod for stirring the reaction liquid; Obtaining detection methodology information of the reaction solution; According to the detection methodology information, and taking into account the differences in detection methodology types, a stirring parameter including at least the stirring rod motion trajectory information is obtained; the stirring parameter is preset or set through an interactive interface; Determine whether the reagent currently added to the reaction solution is a trigger reagent; When it is determined that the reagent currently added to the reaction solution is a trigger reagent, controlling the stirring rod to stir the reaction solution according to the stirring parameter; When the detection methodology information is a first-category detection methodology, the stirring rod motion trajectory information is a two-dimensional motion of the stirring rod in a vertical plane, and the two-dimensional motion direction is clockwise, counterclockwise, or alternating clockwise and counterclockwise. The first-category detection methodology includes immunoturbidimetry and chromogenic substrate method; wherein: When the detection methodology information is a chromogenic substrate method, the obtained closed trajectory is composed of four line segment paths in the same vertical plane, wherein the four line segment paths are respectively a horizontal line segment path, two vertical line segment paths connected to both ends of the horizontal line segment path, and a line segment path connecting the two vertical line segments with the ends that are not connected to the horizontal line segment; When the detection methodology information is immunoturbidimetry, the obtained closed trajectory is composed of two curved paths in the same vertical plane, one of which is a concave curve path and the other is a convex curve path. One end of the concave curve path is connected to one end of the convex curve path, and the other end of the concave curve path is connected to the other end of the convex curve path.

3. A method for stirring a reaction liquid, applied to an in vitro detection device, wherein the in vitro detection device comprises a stirring rod; characterized in that: include: Providing the stirring rod for stirring the reaction liquid; Obtaining detection methodology information of the reaction solution; According to the detection methodology information, and taking into account the differences in detection methodology types, a stirring parameter including at least the stirring rod motion trajectory information is obtained; the stirring parameter is preset or set through an interactive interface; controlling the stirring rod to stir the reaction liquid according to the stirring parameters; in: When the detection methodology information is a chromogenic substrate method, the obtained closed trajectory is composed of four line segment paths in the same vertical plane, wherein the four line segment paths are respectively a horizontal line segment path, two vertical line segment paths connected to both ends of the horizontal line segment path, and a line segment path connecting the two vertical line segments with the ends that are not connected to the horizontal line segment; When the detection methodology information is immunoturbidimetry, the obtained closed trajectory is composed of two curved paths in the same vertical plane, one of which is a concave curve path and the other is a convex curve path. One end of the concave curve path is connected to one end of the convex curve path, and the other end of the concave curve path is connected to the other end of the convex curve path.

4. A method for stirring a reaction liquid, applied to an in vitro detection device, wherein the in vitro detection device comprises a stirring rod; characterized in that: include: Providing the stirring rod for stirring the reaction liquid; Obtaining detection methodology information of the reaction solution; According to the detection methodology information, and taking into account the differences in detection methodology types, a stirring parameter including at least the stirring rod motion trajectory information is obtained; the stirring parameter is preset or set through an interactive interface; Determine whether the reagent currently added to the reaction solution is a trigger reagent; When it is determined that the reagent currently added to the reaction liquid is a trigger reagent, the stirring rod is controlled to stir the reaction liquid according to the stirring parameter; wherein: When the detection methodology information is a chromogenic substrate method, the obtained closed trajectory is composed of four line segment paths in the same vertical plane, wherein the four line segment paths are respectively a horizontal line segment path, two vertical line segment paths connected to both ends of the horizontal line segment path, and a line segment path connecting the two vertical line segments with the ends that are not connected to the horizontal line segment; When the detection methodology information is immunoturbidimetry, the obtained closed trajectory is composed of two curved paths in the same vertical plane, one of which is a concave curve path and the other is a convex curve path. One end of the concave curve path is connected to one end of the convex curve path, and the other end of the concave curve path is connected to the other end of the convex curve path.

5. The stirring method according to claim 3 or 4, wherein: The stirring rod performs a two-dimensional motion in a vertical plane, including: the stirring rod repeatedly performs the motion on the closed track.

6. A stirring mechanism for a reaction liquid, used in an in vitro detection device, characterized in that: include: A stirring rod, used to stir the reaction solution; A driving component, used for driving the stirring rod to move; A processor, configured to execute the stirring method according to any one of claims 1 to 5.

7. The stirring mechanism according to claim 6, wherein: Also included is a container with a non-circular cross section for containing the reaction liquid to cooperate with the stirring rod for stirring.

8. An in vitro detection device, characterized in that: Comprising the stirring mechanism as claimed in claim 6.

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