A biochemical marker on-site instant detection and analysis device based on a level meter

Abstract

The present invention discloses a biochemical marker on-site instant detection and analysis device based on a level meter, comprising a horizontal instrument panel, a base for carrying and fastening the horizontal instrument panel, a plurality of fixed legs and detection legs; the plurality of fixed legs and detection legs are evenly connected to the base, and the horizontal instrument panel is horizontal in a non-working state; the detection legs include a connector connected to the base, a sleeve assembly movably connected to the connector, a sliding mechanism hinged to the sleeve assembly, and a reaction container connected to the sliding mechanism; the sliding mechanism includes a movable slider, and the movable slider is pushed in a working state to drive the horizontal instrument panel on the base to tilt. The present invention can realize on-site instant detection, and the detection process does not require the aid of any other precision instruments, which solves the problem of unclear result judgment in the detection process, improves the sensitivity of the detection, and the device can be reused, which greatly reduces the detection cost.

Description

Technical Field

[0001] The invention relates to the field of chemical analysis, and in particular to an on-site instant detection and analysis device for biochemical markers based on a level meter. Background Art

[0002] The detection process is through signal transduction, chemical signals are converted into signals that can be recognized by the human eye or precision instruments, and detection is achieved by establishing a mathematical relationship between the recognized signal and the unknown target. The recognizable signals generally include light signals, electrical signals, heat, magnetic fields, displacements, and other signals that can be observed directly or with the help of measuring tools.

[0003] For qualitative, semi-quantitative and even quantitative detection of target objects, laboratory tests mostly use precision instruments to capture optical and electrical signals to achieve detection, which has excellent performance such as fast detection speed, high detection throughput, and high detection result accuracy. However, laboratory detection methods are not very applicable in remote areas where equipment is backward but there is an urgent need to obtain test results on the spot. Therefore, point-of-care testing (POCT) came into being. The POCT method has the advantages of instant detection, low cost, and rapid response. Commonly used POCT products on the market include immunochromatography reagent cards, microfluidic chips, etc. However, the existing POCT methods have many shortcomings:

[0004] 1. Compared with laboratory testing methods, existing POCT products such as immunochromatographic reagent cards and microfluidic chips have low stability and sensitivity, and can only meet the basic requirements of POCT rapid testing;

[0005] 2. Existing products must also be used with related instruments for quantitative testing, which is equivalent to miniaturizing laboratory instruments and moving the laboratory to the site. Therefore, the testing process cannot be completed when there is a lack of instruments;

[0006] 3. When doing qualitative testing, the color change of the test area is generally observed with the naked eye. However, different people's eyes have completely different sensitivities to different colors. Therefore, the reading results are also different, and false negative / false positive phenomena may occur;

[0007] 4. The immunochromatographic reagent cards and microfluidic chips in existing products are generally disposable. In addition to the reagent cards and chips, consumables such as centrifuge tubes and pipette tips are also required. Therefore, a large amount of plastic medical waste will be generated during the testing process;

[0008] 5. Existing POCT products such as reagent cards and chips have complex production processes and high production costs;

[0009] 6. Existing POCT products have high requirements for the sample type to be tested. For example, blood samples generally require serum or plasma;

[0010] 7. The chemical reaction in the existing detection process occurs between the liquid and solid phases, and the reaction efficiency is not high. Summary of the invention

[0011] In order to overcome the defects of the prior art described above, the present invention provides a biochemical marker on-site instant detection and analysis device based on a level meter.

[0012] The technical solution adopted by the present invention to solve the problem is:

[0013] A biochemical marker on-site instant detection and analysis device based on a level meter, comprising a level instrument panel, a base for carrying and fastening the level instrument panel, a plurality of fixed legs and a detection leg; the plurality of fixed legs and the detection leg are evenly connected to the base, and the level instrument panel is horizontal in a non-working state;

[0014] The detection support leg comprises a connecting piece connected to the base, a sleeve assembly movably connected to the connecting piece, a sliding mechanism hinged to the sleeve assembly, and a reaction container connected to the sliding mechanism;

[0015] The sliding mechanism comprises a movable sliding block, and the movable sliding block is pushed in a working state to drive the horizontal instrument panel on the base to tilt.

[0016] The on-site instant detection and analysis device for biochemical markers based on a spirit level provided by the present invention can realize on-site instant detection. The detection process does not require the aid of any other precision instruments. The biochemical reaction in the reaction container generates gas. The air pressure formed by the gas in the reaction container pushes the movable slider to vertically displace, thereby causing the horizontal instrument panel on the base to tilt. The bubbles in the horizontal instrument panel are offset, and the presence of the target object can be determined. This solves the problem of unclear result determination during the detection process and improves the sensitivity of the detection. The concentration of the detection object can be determined based on the offset distance of the bubbles in the horizontal instrument panel. The device can be reused, greatly reducing the detection cost.

[0017] Furthermore, the sliding mechanism also includes a slot member; the movable sliding block is arranged in a slide slot of the slot member, and a limit stop block is provided at the bottom of the slide slot.

[0018] Furthermore, the inner cavity of the reaction container is provided with a limiting step for preventing the groove member from sliding down.

[0019] Furthermore, the reaction container is movably connected with a reaction piece, and the reaction piece is arranged to be inclined upward.

[0020] Furthermore, an overflow hole is provided on the reaction member.

[0021] Furthermore, the sleeve assembly includes a first component and a second component, the first component is provided with a first protrusion, and the second component is provided with a second protrusion; the movable slider is provided with a hinge hole, and the hinge hole is hinged to the first protrusion and the second protrusion.

[0022] Furthermore, the sleeve assembly includes a first component and a second component, and the first component and the second component are provided with a penetrating hinge hole; the movable slider is provided with a first protrusion and a second protrusion; the hinge hole is hinged to the first protrusion and the second protrusion.

[0023] Furthermore, the connecting piece, the first component and the second component are combined to form a sliding connection structure.

[0024] Furthermore, the end of the fixed support leg is configured as a spherical structure.

[0025] Furthermore, the base is provided with a plurality of connection grooves for connecting the supporting legs on the circumference, the base is provided with a groove for placing a horizontal instrument panel, and the groove is provided with a loading and unloading hole for easy loading and unloading.

[0026] In summary, the on-site instant detection and analysis device for biochemical markers based on a level meter of the present invention has the following technical effects:

[0027] 1) On-site instant detection can be achieved without the aid of any other precision instruments. The biochemical reaction in the reaction container produces gas. The gas pressure formed in the reaction container pushes the movable slider to move vertically, thereby causing the horizontal instrument panel on the base to tilt. The bubbles in the horizontal instrument panel are offset, and the presence of the target object can be determined. This solves the problem of unclear result determination during the detection process and improves the sensitivity of the detection. The concentration of the target can be determined based on the offset distance of the bubbles in the horizontal instrument panel. The device can be reused, greatly reducing the detection cost.

[0028] 2) An overflow hole is provided on the reaction piece. When the reaction piece is connected to the reaction container, the overflowed solution is discharged from the overflow hole. The solution fills the reaction container so that the gas generated by the biochemical reaction forms air pressure to push the movable slider.

[0029] 3) Through the hinge connection between the sliding mechanism and the sliding connection structure, the detection foot is displaced in the vertical direction, but it will not cause the fixed foot to be displaced in the horizontal direction to affect the detection result.

[0030] 4) The end of the fixed foot is set to a spherical structure so that when the base is tilted, the height of the fixed foot will not change, thereby improving the accuracy of detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1It is a schematic structural diagram of a biochemical marker on-site instant detection and analysis device based on a level meter according to the present invention;

[0032] Figure 2 It is a schematic cross-sectional structure diagram of the detection support leg of the on-site instant detection and analysis device of biochemical markers based on the level meter of the present invention;

[0033] Figure 3 It is a schematic structural diagram of the base of the on-site instant detection and analysis device of biochemical markers based on the level meter of the present invention;

[0034] Figure 4 It is a structural schematic diagram of another embodiment of the on-site instant detection and analysis device of biochemical markers based on a level meter of the present invention.

[0035] Description of reference numerals:

[0036] 1. Horizontal instrument panel; 2. Base; 21. Connecting groove; 22. Recess; 23. Loading and unloading hole; 3. Fixed support foot; 4. Detection support foot; 41. Connector; 42. Socket assembly; 421. First component; 4211. First protrusion; 4212. First pit; 4213. First connecting block; 4214. First through hole; 422. Second component; 43. Sliding mechanism; 431. Movable slider; 4311. Articulated hole; 432. Slot; 4321. Slide; 4322. Limit block; 433. End cover; 44. Reaction vessel; 441. Limit step; 442. Reaction member; 443. Overflow hole; 444. Reaction interface; 445. Plug. DETAILED DESCRIPTION

[0037] For better understanding and implementation, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0038] In the description of the present invention, it should be noted that the terms "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.

[0039] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0040] Embodiment 1

[0041] like Figure 1-3 As shown, the present invention provides a biochemical marker on-site instant detection and analysis device based on a level meter, comprising a level instrument panel 1, a base 2 for carrying and fastening the level instrument panel 1, two fixed legs 3 and a detection leg 4; the two fixed legs 3 and the detection leg 4 are evenly connected to the base 2, and the level instrument panel 1 is horizontal in a non-working state; the detection leg 4 comprises a connecting member 41 connected to the base 2, a sleeve assembly 42 movably connected to the connecting member 41, a sliding mechanism 43 hinged to the sleeve assembly 42, and a reaction container 44 connected to the sliding mechanism 43;

[0042] The sliding mechanism 43 includes a movable slider 431 . The movable slider 431 is pushed in a working state to drive the horizontal instrument panel 1 on the base 2 to tilt.

[0043] The present invention can realize on-site instant detection, and the detection process does not require the aid of any other precision instruments. The biochemical reaction in the reaction container 44 generates gas, and the air pressure formed by the gas in the reaction container 44 pushes the movable slider 431 to vertically displace, thereby driving the horizontal instrument panel 1 on the base 2 to tilt. The bubbles in the horizontal instrument panel 1 are offset, and the presence of the target object can be determined, which solves the problem of unclear result determination during the detection process and improves the sensitivity of the detection. The concentration of the target object can be determined based on the offset distance of the bubbles in the horizontal instrument panel 1. The device can be reused, greatly reducing the detection cost.

[0044] Specifically, the sliding mechanism 43 further includes a groove 432 and an end cap 433; the movable slider 431 is arranged in the slide groove 4321 of the groove 432, the bottom of the slide groove 4321 is provided with a limit stopper 4322, and the end cap 433 is arranged at the top of the groove 432. The inner cavity of the reaction container 44 is provided with a limit step 441 for blocking the groove 432 from sliding down. The reaction container 44 is provided with a reaction interface 444, and the reaction interface 444 is movably connected with a reaction piece 442, and the reaction piece 442 is arranged tilted upward. The reaction piece 442 is provided with an overflow hole 443, and a hole plug 445 is provided in the overflow hole 443. When the reaction piece 442 is connected to the reaction container 44, the overflowed solution is discharged from the overflow hole 443, and the solution fills the reaction container 44, so that the gas generated by the biochemical reaction forms air pressure to push the movable slider 431.

[0045] In this embodiment, the socket assembly 42 includes a first component 421 and a second component 422, and the first component 421 and the second component 4 are connected to each other. The first component 421 is provided with a first protrusion 4211, a first groove 4212 and a first connecting block 4213, the first protrusion 4211 is provided with a first through hole 4214, the second component 422 is provided with a second groove and a second protrusion, and the second protrusion is provided with a second through hole; the movable slider 431 is provided with a hinge hole 4311, and the hinge hole 4311 forms a hinge connection with the first protrusion 4211 and the second protrusion, and is fixed with bolts. Alternatively, the sleeve assembly 42 includes a first component 421 and a second component 422, wherein the first component 421 is provided with a first pit 4212 and a first connecting block 4213, and the second component 422 is provided with a second pit and a second connecting block, and the first component 421 and the second component 422 are provided with a hinge hole 4311 penetrating the first component 421 and the second component 422; the movable slider 431 is provided with a first protrusion 4211 and a second protrusion, the first protrusion 4211 is provided with a first through hole 4214, and the second protrusion is provided with a second through hole; the hinge hole 4311 forms a hinge connection with the first protrusion 4211 and the second protrusion, and is fixed by bolts. The first component 421 and the second component 422 are combined together so that the first pit 4212 and the second pit form a sleeve sliding groove.

[0046] Specifically, the connecting member 41, the first member 421 and the second member 422 are combined to form a sliding connection structure. According to the above structure, the hinge connection between the sliding mechanism 43 and the sliding connection structure causes the detection leg 4 to be displaced in the vertical direction, but does not cause the fixed leg 3 to be displaced in the horizontal direction to affect the detection result.

[0047] In this embodiment, the end of the fixed support leg 3 is set to a spherical structure, so that when the base 2 is tilted, the height of the fixed support leg 3 will not change, thereby improving the accuracy of detection. The base 2 is provided with three connecting grooves 21 for connecting the support legs on the circumference, and the base 2 is provided with a groove 22 for placing the horizontal instrument panel 1, and the groove 22 is provided with a loading and unloading hole 23 for easy loading and unloading.

[0048] The working principle of the present invention is:

[0049] The device is placed on a horizontal workbench so that it can keep the horizontal instrument panel 1 in a horizontal state before working; the reaction container 44 is filled with hydrogen peroxide solution, the reaction piece 443 is marked with monoclonal antibody 1, and the monoclonal antibody 2 is modified with horseradish peroxidase (HRP) and stored in a buffer solution. After the test sample is added to the buffer solution, the target antigen reacts with the monoclonal antibody 2 to obtain a target antigen-monoclonal antibody 2-HRP complex. After the reaction piece 443 is inserted into the buffer solution, the monoclonal antibody 1 on 443 reacts with the target antigen-monoclonal antibody 2-HRP to generate a monoclonal Antibody 1-target antigen-monoclonal antibody 2-HRP and fixed on reaction piece 443, after reaction piece 443 is washed with buffer solution, it is inserted into reaction container 44, HRP on reaction piece 443 catalyzes hydrogen peroxide to produce oxygen, causing the air pressure in reaction container 44 to rise, pushing movable slider 431 to shift in the vertical direction, thereby driving base 2 and horizontal instrument panel 1 on base 2 to tilt, and water bubbles in horizontal instrument panel 1 to shift, the degree of water bubble shift has a certain correlation with the concentration of target in the sample, and the concentration of target is determined by calculating the shift distance of water bubbles. Monoclonal antibody 1 and monoclonal antibody 2 alone do not react with hydrogen peroxide solution.

[0050] Among them, the process of detecting the target object is: taking a certain amount of sample and adding it to a test tube containing monoclonal antibody II-HRP solution, after shaking, the target object is marked on monoclonal antibody II; monoclonal antibody I is marked on reaction piece 443, and reaction piece 443 is inserted into a test tube containing monoclonal antibody II-HRP solution and shaken and evenly shaken, and placed on a shaker for 5 minutes and then removed. During this period, monoclonal antibody II marked with the target object and monoclonal antibody I undergo an antigen-antibody immune reaction and are connected together; reaction piece 443 is taken out and inserted into a washing liquid test tube for washing for 1 minute, and after removing monoclonal antibody II-HRP that is not marked with the target object, it is inserted into reaction container 44 again, and the degree of displacement of the bubble on the horizontal instrument panel 1 is read after 3 minutes.

[0051] The degree of bubble displacement is correlated with the concentration of the target in the sample, and the correlation is a functional relationship deduced from a large amount of experimental data in the laboratory.

[0052] In addition, the double antibody sandwich method of monoclonal antibody 1 and monoclonal antibody 2 can be replaced by magnetic microspheres. The reaction cup is filled with magnetic microsphere-monoclonal antibody 1 and monoclonal antibody 2-HRP solution. After the sample is added, it is mixed for 3 minutes and washed 3 times under magnetic adsorption. After the monoclonal antibody 2-HRP without labeled target is removed, the mixed solution in the reaction cup is poured into the reaction container 44, and then hydrogen peroxide solution is added. The reaction interface 442 is sealed with the reaction piece 443. After 3 minutes, the distance of the bubble offset in the horizontal instrument panel 1 is read.

[0053] In addition to the above-mentioned method of marking the target, the immune reaction of the antigen and antibody can also be converted into a base complementary pairing reaction of the nucleic acid chain, which can also achieve the goal of marking the target and loading it into the reaction container 44 to analyze the concentration of the target.

[0054] In addition to being applicable to on-site instant testing in remote areas where the equipment is backward but the test results are urgently needed on-site, the present invention can also be used for laboratory diagnosis and bedside testing in clinical departments. At the same time, by adjusting the solution in test tube A, the present invention can be applied to a variety of sample types including undiluted venous blood. The present invention uses enzyme catalysis to amplify the detection signal, making it more sensitive; the data read is the distance that the bubble moves on the horizontal instrument panel 1, and compared with other qualitative or semi-quantitative tests, its results are more objective and true.

[0055] Embodiment 2

[0056] like Figure 4 As shown, the difference between the second embodiment and the first embodiment is that the base 2 is provided with four connection grooves 21 for connecting the legs, and correspondingly there are three fixed legs 3, so that the device can still keep the horizontal instrument panel 1 level in the absence of the detection legs 4, thereby reducing the difficulty of adjusting the level of the horizontal instrument panel 1.

[0057] The technical means disclosed in the scheme of the present invention are not limited to the technical means disclosed in the above-mentioned implementation mode, but also include technical schemes composed of any combination of the above-mentioned technical features. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications are also regarded as the protection scope of the present invention.

Claims

1. A biochemical marker on-site instant detection and analysis device based on a level meter, characterized in that: The horizontal instrument panel (1) comprises a base (2) for supporting and fastening the horizontal instrument panel (1), a plurality of fixed legs (3) and a detection leg (4); the plurality of fixed legs (3) and the detection leg (4) are evenly connected to the base (2); the horizontal instrument panel (1) is horizontal in a non-working state; The detection support foot (4) comprises a connecting piece (41) connected to the base (2), a sleeve assembly (42) movably connected to the connecting piece (41), a sliding mechanism (43) hingedly connected to the sleeve assembly (42), and a reaction container (44) connected to the sliding mechanism (43); The sliding mechanism (43) comprises a movable sliding block (431), and the movable sliding block (431) is pushed in a working state to drive the horizontal instrument panel (1) on the base (2) to tilt; The end of the fixed support foot (3) is configured as a spherical structure; When conducting instant testing on site, the biochemical reaction in the reaction container (44) generates gas, and the gas pressure generated by the gas in the reaction container (44) pushes the movable slider (431) to move vertically, thereby causing the base (2) and the horizontal instrument panel (1) on the base (2) to tilt, and the bubbles in the horizontal instrument panel (1) to shift, thereby determining the presence of the target object in the sample.

2. The on-site instant detection and analysis device for biochemical markers based on a level meter according to claim 1, characterized in that: The sliding mechanism (43) further comprises a slot member (432), the movable sliding block (431) is arranged in a sliding slot (4321) of the slot member (432), and a limit stopper (4322) is provided at the bottom of the sliding slot (4321).

3. The on-site instant detection and analysis device for biochemical markers based on a level meter according to claim 2, characterized in that: The inner cavity of the reaction container (44) is provided with a limiting step (441) for preventing the groove member (432) from sliding downward.

4. The on-site instant detection and analysis device for biochemical markers based on a level meter according to claim 3 is characterized in that: The reaction container (44) is movably connected to a reaction piece (442).

5. The on-site instant detection and analysis device for biochemical markers based on a level meter according to claim 4, characterized in that: The reaction piece (442) is provided with an overflow hole (443).

6. The on-site instant detection and analysis device for biochemical markers based on a level meter according to claim 5, characterized in that: The sleeve assembly (42) comprises a first component (421) and a second component (422); the first component (421) is provided with a first protrusion (4211), and the second component (422) is provided with a second protrusion; the movable sliding block (4311) is provided with a hinge hole (4311), and the hinge hole (4311) is hinged to the first protrusion (4211) and the second protrusion.

7. The on-site instant detection and analysis device for biochemical markers based on a level meter according to claim 5, characterized in that: The sleeve assembly (42) comprises a first component (421) and a second component (422); the first component (421) and the second component (422) are provided with a penetrating hinge hole (4311); the movable slider (431) is provided with a first protrusion (4211) and a second protrusion; the hinge hole (4311) is hingedly connected to the first protrusion (4211) and the second protrusion.

8. The on-site instant detection and analysis device for biochemical markers based on a level meter according to claim 6 or 7, characterized in that: The connecting member (41), the first component (421) and the second component (422) are combined to form a sliding connection structure.

9. The on-site instant detection and analysis device for biochemical markers based on a level meter according to claim 1, characterized in that: The base (2) is provided with a plurality of connection grooves (21) for connecting the legs in the circumferential direction, and the base (2) is also provided with a groove (22) for placing the horizontal instrument panel (1), and the groove (22) is provided with a loading and unloading hole (23) for facilitating loading and unloading.

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