Preparation and application of dimethyl imidazole cobalt modified manganese dioxide microspheres

By immobilizing acetylcholinesterase on manganese dioxide microspheres modified with cobalt dimethylimidazolium and combining it with capillary electrophoresis, the stability and efficiency issues of screening acetylcholinesterase inhibitors from traditional Chinese medicine components have been solved, achieving efficient screening and low-cost discovery of acetylcholinesterase inhibitors.

CN115197929BActive Publication Date: 2026-07-03LANZHOU INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LANZHOU INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES
Filing Date
2022-08-09
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing technologies are insufficient for efficiently screening traditional Chinese medicine components with acetylcholinesterase inhibitory activity, and traditional immobilized enzyme methods suffer from inadequate stability and reusability.

Method used

Using cobalt dimethylimidazolium modified manganese dioxide microspheres as a carrier, acetylcholinesterase was immobilized through electrostatic interaction, and acetylcholinesterase inhibitors were screened from traditional Chinese medicine using capillary electrophoresis.

Benefits of technology

It achieves efficient and stable screening of acetylcholinesterase inhibitors, has good pH and thermal stability, strong reusability, high separation efficiency, low sample consumption, and low operating cost.

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Abstract

The application provides a preparation method of dimethyl imidazole cobalt modified manganese dioxide microspheres, which comprises the following steps: completely dissolving manganese dioxide microspheres in a methanol solution of cobalt nitrate, adding a methanol solution of 2-methyl imidazole, stirring, centrifuging, washing, and drying to obtain dimethyl imidazole cobalt modified manganese dioxide microspheres. The material has the advantages of easy synthesis, high specific surface area, high loading capacity, good permeability, short diffusion path, excellent thermal stability and the like. As a new type of enzyme immobilization carrier, acetylcholinesterase is immobilized on the dimethyl imidazole cobalt modified manganese dioxide microspheres through electrostatic interaction. The application also provides the application of the immobilized acetylcholinesterase in combination with capillary electrophoresis technology in screening of acetylcholinesterase inhibitors from Chinese traditional medicine Inula japonica. The immobilized acetylcholinesterase has the advantages of wide pH resistance, good thermal stability, reusability, high enzyme activity and the like.
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