POCT hematology analyzer and method of using same

Abstract

The application provides a POCT blood cell analyzer, which comprises a rack, a detection seat and a pipette. The detection seat is arranged in sliding or rotating mode relative to the rack. The detection seat reciprocates between a loading station and a sample detection station. When the detection seat slides out or rotates out of the rack, it is located at the loading station to receive a reagent box or allow the reagent box to be taken out. The pipette is arranged in the shell and above the detection seat to perform corresponding operations on the reagent box.

Description

Technical Field

[0001] This application relates to the field of medical device technology, and in particular to a POCT hematology analyzer, a pipette, a testing stand, a method for using the POCT hematology analyzer, a reagent kit, a mounting stand, a sample testing device, and a microplate. Background Technology

[0002] A hematology analyzer, also known as a blood cell counter, blood cell analyzer, or blood cell analyzer, is one of the most widely used instruments in hospital clinical laboratories. A large portion of the components in a traditional hematology analyzer belong to the cleaning system, because before the next sample tube is tested, all traces of previous use must be thoroughly cleaned from the tube. The entire cleaning system is not only complex in structure and has many components, but the cleaning process also requires a large amount of reagents and takes a considerable amount of time.

[0003] POCT blood cell analyzers have greatly simplified instrument components compared to traditional blood cell analyzers. POCT blood cell analyzers can completely eliminate the cleaning fluid circuit components in traditional blood analysis, greatly reducing the complexity of the instrument and production costs.

[0004] However, some existing POCT blood cell analyzers have overly simple functions, some have complex structures, some have low levels of automation, and some are quite expensive. Summary of the Invention

[0005] This application provides a POCT hematology analyzer, a pipette, a test stand, a method for using the POCT hematology analyzer, a reagent kit, a mounting base, a sample testing device, and a microplate, to at least partially solve the above-mentioned technical problems.

[0006] To solve the above-mentioned technical problems, one technical solution adopted in this application is: to provide a POCT blood cell analyzer, the POCT blood cell analyzer including a frame, a test seat, and a pipette, the test seat being slidably or rotatably disposed relative to the frame, the test seat reciprocating between a loading station and a sample testing station, the test seat being positioned at the loading station when sliding out or rotating out of the frame to receive reagent kits or allow reagent kits to be removed; the pipette is disposed inside the housing and above the test seat, for performing corresponding operations on the reagent kits.

[0007] The beneficial effects of this application are as follows: Unlike the prior art, the POCT blood cell analyzer, pipette, test stand, method of using the POCT blood cell analyzer, reagent kit, assembly stand, sample detection device, and microplate structure provided by this application are novel, practical, reliable, low-cost, and can efficiently complete fully automated detection. Attached Figure Description

[0008] To more clearly illustrate the technical solutions in this embodiment, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort, wherein:

[0009] Figure 1 This is a three-dimensional structural schematic diagram of a POCT blood cell analyzer provided in an embodiment of this application;

[0010] Figure 2 This is a schematic diagram of the module structure of a POCT blood cell analyzer provided in one embodiment of this application;

[0011] Figure 3 This is a schematic diagram of the internal structure of a POCT blood cell analyzer provided in one embodiment of this application;

[0012] Figure 4 This is a schematic diagram of the internal structure of a POCT blood cell analyzer provided in an embodiment of this application, wherein the pipette is omitted;

[0013] Figure 5 This is a schematic diagram of the air circuit structure of a pressure-building system provided in an embodiment of this application;

[0014] Figure 6 This is a three-dimensional structural schematic diagram of a pipette provided in an embodiment of this application;

[0015] Figure 7 This is a three-dimensional structural schematic diagram of a puncture head provided in an embodiment of this application;

[0016] Figure 8 This is a bottom view of the metal shielding cover and transmission mechanism of a POCT blood cell analyzer provided in an embodiment of this application.

[0017] Figure 9 This is a three-dimensional structural schematic diagram of the detection seat of a POCT blood cell analyzer provided in an embodiment of this application;

[0018] Figure 10 This is an exploded structural diagram of the detection seat of a POCT blood cell analyzer provided in one embodiment of this application;

[0019] Figure 11 This is a side view of the conductive support and conductive post of a POCT blood cell analyzer provided in another embodiment of this application;

[0020] Figure 12 This is a top view of the reagent kit provided in one embodiment of this application;

[0021] Figure 13 This is a simplified three-dimensional perspective structural diagram of a POCT blood cell analyzer provided in another embodiment of this application;

[0022] Figure 14 This is a flowchart illustrating the use of a POCT blood cell analyzer provided in one embodiment of this application;

[0023] Figure 15 This is a schematic diagram of the exploded structure of a reagent kit provided in an embodiment of this application;

[0024] Figure 16 This is an exploded structural diagram of a reagent kit provided in one embodiment of this application from another perspective;

[0025] Figure 17 This is a cross-sectional structural schematic diagram of a reagent kit provided in an embodiment of this application from one perspective;

[0026] Figure 18 This is a cross-sectional structural schematic diagram from another perspective of the reagent kit provided in one embodiment of this application;

[0027] Figure 19 This is a three-dimensional structural schematic diagram of a microporous sheet provided in an embodiment of this application;

[0028] Figure 20 yes Figure 19 The diagram shows a cross-sectional structure of the microporous sheet. Detailed Implementation

[0029] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0030] It should be noted that if the embodiments of this application involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0031] Furthermore, if the embodiments of this application involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. If the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0032] This application provides a POCT blood cell analyzer and its usage method.

[0033] like Figures 1 to 14 As shown, the POCT blood cell analyzer includes a housing 10, a display screen 11, an outlet 12, a syringe 220, a frame 301, a pressure building system, a test base 300 and its transmission mechanism, a pipette 400 and its transmission mechanism, a metal shielding cover 500 and its transmission mechanism, etc.

[0034] The test chamber 300 can reciprocate in and out of the outlet 12 to facilitate the loading or unloading of the reagent kit 20. The pipette 400 and its transmission mechanism are used to achieve fully automated pretreatment of the sample to be tested (which can be pre-loaded on the reagent kit 20). The fully automated pretreatment includes automatic sample addition, automatic reagent addition (the reagent kit 20 is pre-loaded with diluent, hemolysin, staining and lysis reagent, etc.), and automatic mixing (aeration or stirring). The pressure building system is used to provide the positive and negative pressure required for the pipette 400 to perform fully automated pretreatment. The metal shielding cover 500 and its transmission mechanism are also included. This device is used in conjunction with a pressure-building system for automatic detection. Automatic detection refers to the process where, when the metal shielding cover 500 and the detection base 300 are closed to form an electromagnetic shielding space, the pneumatic connection device 510 of the pressure-building system connects to the pressure chamber 140 on the reagent kit 20 (the pressure chamber 140 is connected to the rear chamber of the impedance detection cell). The pressure chamber 210 of the pressure-building system then provides negative or positive pressure, causing the liquid in the front chamber 120 of the impedance detection cell of the reagent kit 20 to flow through micropores to the rear chamber of the impedance detection cell of the reagent kit 20, and recording relevant parameters during this process. The pneumatic connection device 510 of the pressure-building system is connected to and moves synchronously with the metal shielding cover 500.

[0035] The pressure building system includes a pressure chamber 210, a first injector 222 and a second injector 223 that are linked together, a drive unit 224, an air filter 225, a first solenoid valve SV1, a second solenoid valve SV2, a third solenoid valve SV3, and a pneumatic connection device 510, etc.

[0036] The pipette 400 includes a gas delivery tube 401, an outer sleeve 402 fitted around the gas delivery tube 401, a synchronization block 403, a light-blocking plate 404, an optocoupler 405, a fixing plate 406, a through groove 407, and a blocking component 4031 for unloading the mounting head (201 or 204), etc.

[0037] The metal shielding cover 500 is mounted on the frame 301 via the mounting bracket 520 and moves vertically up and down via the motor 530 and guide column 540. The metal shielding cover 500 is a cover with an opening at the bottom, and the pneumatic connection device 510 connected to the pressure building system extends into the interior of the metal shielding cover 500.

[0038] The detection base 300 includes a main body 310, a conductive support 303 connected to the main body 310, a metal shielding base (320, 321, 322) covering the main body 310, an optical detection component 330 embedded in the main body 310, a Peltier 340 arranged sequentially near the detection cavity of the optical detection component 330, a heat sink 350, and a fan 360.

[0039] The following will provide specific examples of various implementation methods for the POCT blood cell analyzer.

[0040] First embodiment, such as Figures 1 to 13 As shown, this embodiment provides a POCT blood cell analyzer, which includes a housing 10, a detection base 300, and a pipette 400.

[0041] The detection holder 300 can extend into or out of the housing 10 to receive the reagent kit 20 equipped with an impedance detection cell. The detection holder 300 is equipped with a power supply component (e.g., for impedance detection) to assist in impedance detection. Figure 9 The conductive support 303 shown is illustrated. The kit 20 has an installation head receiving pool for mounting the installation head (tip head 201 or puncture head 204).

[0042] The pipette 400 is housed within the housing 10 and positioned above the detection seat 300. It is used to perform operations on the reagent kit 20, including pipetting, aeration, mixing, and puncturing the sealing membrane. When the mounting head needs to be unloaded (including during normal testing and unexpected power outages), the pipette 400 moves to a preset position outside the mounting head's containment cell (e.g., ...). Figure 12 The forepool 120 in the middle can perform the installation head unloading operation to ensure that the pipette 400 will not be repositioned with the installation head when the installation head container is already equipped with the installation head in the event of an unexpected power outage and restart. It can also shorten the running path when unloading the installation head and reduce the alignment requirements when unloading the installation head (generally the diameter of the installation head container is relatively small, and precise alignment is required when unloading).

[0043] This embodiment also provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400, and a detector (which may be a detection element such as an optocoupler 405).

[0044] The test holder 300 extends into or out of the housing 10 to receive the reagent kit 20, which is equipped with an impedance detection cell. The test holder 300 is equipped with a power supply for impedance detection. The reagent kit 20 has a mounting head receiving cell for mounting the mounting head.

[0045] The pipette 400 is located inside the housing 10 and above the detection seat 300, and is used to perform corresponding operations on the reagent kit 20.

[0046] The detector is used to detect whether a mounting head is loaded on the pipette 400 when the POCT blood cell analyzer needs to unload the mounting head. If a mounting head is detected on the pipette 400, the pipette 400 will move to the mounting head container or a preset position elsewhere to perform the mounting head unloading operation. By setting the detector, it is possible to know whether a mounting head is loaded on the pipette 400 and then selectively perform the unloading operation, which is relatively more intelligent.

[0047] This embodiment also provides a POCT hematology analyzer for receiving reagent kit 20. The POCT hematology analyzer includes a test stand 300 and a pipette 400.

[0048] The test holder 300 is used to receive the reagent kit 20, which is equipped with an impedance detection cell, and the test holder 300 is equipped with a power supply component for impedance detection. The reagent kit 20 has a mounting head receiving cell for mounting the mounting head.

[0049] The pipette 400 is positioned above the test holder 300 and is used to load the mounting head for corresponding operations on the reagent kit 20. When the mounting head needs to be unloaded, the pipette 400 moves to the mounting head container or a preset position outside the mounting head container to perform the mounting head unloading operation. In this embodiment, the POCT hematology analyzer also includes a detector (which may be a detection element such as an optical coupler 405). The detector is used to detect whether the pipette 400 is loaded with a mounting head when the POCT hematology analyzer needs to unload the mounting head. If a mounting head is detected on the pipette 400, the pipette 400 unloads the mounting head to the mounting head container or a preset position outside the mounting head container. Unloading the mounting head to a preset position outside the mounting head container is simpler in operation, as it directly finds the nearest large-diameter container for unloading. This avoids repeatedly placing the mounting head when it is already loaded in the mounting head container, shortens the running path when unloading the mounting head, and reduces the alignment requirements when unloading the mounting head.

[0050] The reagent kit 20 has multiple compartments, with one compartment pre-positioned. These compartments include a connector compartment that matches the connector and several functional compartments. The pipette 400 unloads the connector into any of the functional compartments. The functional compartments may include a diluent compartment 111, a fore-cell compartment 120 (for WBC or RBC detection), etc. By unloading the connector into any functional compartment, the pipette 400 effectively selects a compartment with an absolutely empty space and a relatively large opening.

[0051] like Figure 6 As shown, the pipette 400 includes a gas delivery tube 401 and an outer tube 402. When the outer tube 402 moves axially relative to the gas delivery tube 401, the mounting head can be unloaded.

[0052] This embodiment also provides a method for using a POCT blood cell analyzer, the method including:

[0053] The pipette 400 of the POCT blood cell analyzer moves to the mounting head container to load the mounting head for the first test.

[0054] The first test may complete normally or experience an unexpected power outage. Before performing a second test, the mounting head used in the first test needs to be unloaded. The pipette 400 of the POCT hematology analyzer moves to a preset position outside the mounting head container to perform the mounting head unloading operation. This method ensures that the pipette 400 does not reposition the mounting head when it is already in the mounting head container, shortens the travel path during mounting head unloading, and reduces the alignment requirements during mounting head unloading.

[0055] This embodiment also provides a method for using a POCT blood cell analyzer, the method including:

[0056] The pipette 400 of the POCT blood cell analyzer moves to the mounting head container to load the mounting head for the first test.

[0057] The first test may be completed normally or due to an unexpected power outage. Before the second test, the mounting head used in the first test needs to be unloaded. You can check whether the mounting head is loaded on the pipette 400 of the POCT blood cell analyzer by using a detector.

[0058] If a mounting head is detected on the pipette 400, the pipette 400 moves to a preset position outside the mounting head container to perform a mounting head unloading operation.

[0059] This method of use can determine whether a mounting head is loaded on the pipette 400 and then selectively perform an unloading operation, making it relatively more intelligent.

[0060] This embodiment also provides a method for using a POCT blood cell analyzer, including:

[0061] The pipette of the POCT hematology analyzer moves to the mounting head container to load the mounting head for the first test.

[0062] The first test may be completed normally or due to an unexpected power outage. Before the second test, the reagent kit 20 and the mounting head used in the first test need to be removed. Therefore, it is necessary to check whether the POCT blood cell analyzer is equipped with the reagent kit 20.

[0063] Before performing a second test, the device can be used to check whether the pipette 400 of the POCT blood cell analyzer is loaded with a mounting head.

[0064] If the POCT hematology analyzer detects that the reagent kit 20 is installed and the pipette 400 is loaded with an installation head, the pipette 400 is controlled to move to the installation head container or a preset position outside the installation head container to perform the installation head unloading operation. When performing the installation head unloading operation at a preset position outside the installation head container, the installation head will not be placed again if the installation head container is already loaded with an installation head. This also shortens the running path when unloading the installation head and reduces the alignment requirements when unloading the installation head.

[0065] The preset position can correspond to the inside of the detection socket 300 or the outside of the detection socket 300. That is to say, the unloading operation is not limited to unloading the mounting head onto the detection socket 300 or onto the reagent kit 20, but can also be unloaded directly into an area inside the instrument other than the detection socket 300. For example, there is a mounting head recycling box inside the instrument, which maintenance personnel can empty regularly.

[0066] The mounting head can be unloaded by axially moving the outer tube 402 of the pipette 400 relative to the gas inlet tube 401 of the pipette 400. In a specific embodiment, the pipette 400 unloads the mounting head by a fixedly installed blocking member or a retractable blocking member 4031 that moves with it. The blocking member 4031 can be a telescopic motor, a vertically or horizontally installed electromagnet, or a U-shaped baffle. A synchronizing block 403 can be connected to the outer tube 402. When the blocking member 4031 blocks the synchronizing block 403 and the pipette 400 rises, the mounting head is unloaded by the lower end of the outer tube 402.

[0067] Please refer to the second embodiment as well. Figures 1 to 13 This embodiment provides a pipette 400, which includes an air delivery tube 401 and a detection element.

[0068] The air tube 401 is used to load the installation head (tip head 201 or puncture head 204). The detection element is located on the side of the air tube 401 and is used to detect whether the installation head is loaded and connected on the air tube 401.

[0069] The pipette 400 also includes an outer tube 402, which is sleeved on the outer periphery of the gas delivery tube 401. When the gas delivery tube 401 is loaded with the installation head, the gas delivery tube 401 is inserted into the installation head, so that the installation head pushes the outer tube 402 to change position along the axial direction of the gas delivery tube 401. The detection element is used to detect the position of the outer tube 402 and thus determine whether the installation head is sleeved on the gas delivery tube 401.

[0070] When the installation head is fitted onto the air guide tube 401, the outer sleeve 402 is located in the first position of the air guide tube 401. Figure 6 (As shown in the diagram) When the installation head is not attached to the air duct 401, the outer tube 402 is located in the second position of the air duct 401, and the first position is higher than the second position.

[0071] A light-blocking plate 404 is connected to the outer tube 402. The detection component is an optical coupler 405 that is matched with the light-blocking plate 404. When the light channel of the optical coupler 405 is blocked by the light-blocking plate 404, it indicates that an installation head is sleeved on the air guide tube 401. When the light channel of the optical coupler 405 is not blocked by the light-blocking plate 404, it indicates that no installation head is sleeved on the air guide tube 401.

[0072] The pipette 400 includes a fixing plate 406 disposed on the side of the gas delivery tube 401, and an optocoupler disposed on the fixing plate 406. The gas delivery tube 401 of the pipette 400 can move up and down independently, and the pipette 400 as a whole can move in two or three dimensions.

[0073] The fixing plate 406 is provided with a through groove 407. A pair of optical transceivers of the optical coupler 405 extend into the through hole and are located on the side of the air duct 401. A synchronization block 403 is also connected to the outer sleeve 402. The synchronization block 403 extends into the through groove 407. When the air duct 401 rises and the synchronization block 403 is blocked, the mounting head is unloaded. After the mounting head is unloaded, the outer sleeve 402 is still sleeved on the outer periphery of the air duct 401. Since the synchronization block 403 extends into the through groove 407, the outer sleeve 402 will not fall off relative to the air duct 401, but will be naturally supported at the bottom of the through groove 407 (i.e., the second position).

[0074] This embodiment also provides a method for using a POCT blood cell analyzer, including the following steps:

[0075] Before performing pipetting, determine whether the gas delivery tube 401 of the pipette 400 is equipped with an installation head;

[0076] An alarm is triggered if the pipette 400's air delivery tube 401 is not fitted with a mounting tip. Since the tubing in a POCT hematology analyzer is entirely gaseous, not liquid, liquid cannot be aspirated from the tubing. Therefore, the air delivery tube 401 must be fitted with a mounting tip (tip 201) to perform the necessary operations. Thus, it is necessary to check whether the pipette 400's air delivery tube 401 is fitted with a mounting tip.

[0077] In the step of detecting whether the gas delivery tube 401 of the pipette 400 is equipped with an installation head, an outer sleeve 402 is fitted around the outer periphery of the gas delivery tube 401. The position of the outer sleeve 402 is detected by the detection element to determine whether the gas delivery tube 401 is fitted with an installation head.

[0078] When the installation head is fitted onto the air guide tube 401, the outer tube 402 is located in the first position of the air guide tube 401. When the installation head is not fitted onto the air guide tube 401, the outer tube 402 is located in the second position of the air guide tube 401. The first position is higher than the second position.

[0079] The detection component is an optocoupler 405, and a light-blocking plate 404 matching the optocoupler 405 is provided on the outer sleeve 402. When the light-blocking plate 404 blocks the optical channel of the optocoupler 405, it is determined that the air guide tube 401 is fitted with the mounting head outer sleeve 402.

[0080] This embodiment provides a POCT blood cell analyzer, including a housing 10, a detection base 300, a pipette 400, and a detection component.

[0081] The detection seat 300 can extend into or out of the housing 10 for receiving the reagent kit 20 equipped with an impedance detection cell; the pipette 400 is disposed inside the housing 10 and located above the detection seat 300. The pipette 400 includes an air guide tube 401 for loading the mounting head (tip head 201 or puncture head 204); a detection element is disposed on the side of the air guide tube 401 for detecting whether the mounting head is loaded on the air guide tube 401. The detection element can be an optical coupler 405 or other detection element that can detect the presence or absence of an object.

[0082] In this embodiment, the detection device can detect the loading status of the mounting head, thus preventing the pipette from being used for pipetting operations without the mounting head loaded, which could contaminate the internal gas path of the POCT blood cell analyzer.

[0083] Please refer to the third embodiment as well. Figures 1 to 13This embodiment provides a POCT blood cell analyzer, which includes a test seat 300, a pipette 400 disposed above the test seat 300, and a pressure building system. The test seat 300 is used to receive the reagent kit 20. The reagent kit 20 has a front chamber 120 and a rear chamber connected by micropores. The pressure building system includes a pressure chamber 210, a pneumatic connection device 510, a first syringe 222, a second syringe 223, and a drive component 224.

[0084] The first syringe 222 is connected to the pressure chamber 210 or the pipette 400 and is used to establish positive or negative pressure in the pressure chamber 210. It is also used to assist the pipette 400 in pipetting or to aerate and mix the liquid in the fore chamber 120. The second syringe 223 is connected to the pipette 400 and is used to aspirate or expel samples or reagents. The drive unit 224 is used to drive the first syringe 222 and the second syringe 223 simultaneously. The first syringe 222 and the second syringe 223 can be linked syringes to facilitate sharing the same drive unit 224. The drive unit 224 can be a motor. Of course, the first syringe 222 and the second syringe 223 can also be separate syringes and controlled independently by different motors.

[0085] The POCT blood cell analyzer also includes solenoid valves, which include a first solenoid valve SV1, a second solenoid valve SV2, and a third solenoid valve SV3.

[0086] The first solenoid valve SV1 selectively connects the first syringe 222 to the pressure chamber 210 and the second syringe 223; the second solenoid valve SV2 selectively connects the first solenoid valve SV1 to the pressure chamber 210 and the outside atmosphere, and the outside atmosphere is connected to the second solenoid valve SV2 through the air filter 225 and the pipeline T10; the third solenoid valve SV3 selectively connects the pneumatic connection device 510 to the pressure chamber 210, and when the third solenoid valve SV3 is energized, the pipelines T8 and T7 are connected through the third solenoid valve SV3.

[0087] The first syringe 222 can draw in air through the air filter 225, T10 line, second solenoid valve SV2, T5 line, first solenoid valve SV1, and T1 line, and then establish positive pressure in the pressure chamber 210 through the T1 line, first solenoid valve SV1, T5 line, second solenoid valve SV2, and T6 line.

[0088] The first syringe 222 can draw away the gas in the pressure chamber 210 through the T1 line, the first solenoid valve SV1, the T5 line, the second solenoid valve SV2, and the T6 line to establish a negative pressure, and then exhaust the gas through the T1 line, the first solenoid valve SV1, the T5 line, the second solenoid valve SV2, the T10 line, and the air filter 225.

[0089] After establishing positive or negative pressure in pressure chamber 210, positive or negative pressure can be output through pipeline T7, third solenoid valve SV3, pipeline T8, and pneumatic connection device 510. Among them, pipeline T8 can be a relatively rigid pipeline, that is, the rigidity of pipeline T8 is greater than that of the second conduit 221. The greater rigidity of pipeline T8 can prevent the pipe walls of pipeline T8 from sticking together and blocking the internal airflow channel of pipeline T8 when outputting negative pressure.

[0090] When the first solenoid valve SV1 connects the T1 line and the T2 line, the positive or negative pressure generated by the first syringe 222 and the second syringe 223 simultaneously acts on the pipette 400. That is, when a large amount of sample needs to be aspirated or pushed, the first syringe 222 can play an auxiliary role in pipetting.

[0091] The volume of the first syringe 222 is larger than that of the second syringe 223. The first syringe 222 is mainly used for pressure building, and the second syringe 223 is mainly used for sample pushing / absorption. Of course, both can be used simultaneously for pressure building or sample pushing / absorption as needed.

[0092] The volume ratio of the first syringe 222 to the second syringe 223 can be 80 to 120:1. For example, the volume of the first syringe 222 is 10 ml, and the volume of the second syringe 223 is 100 μL. The drive unit 224 drives the piston rods of the first syringe 222 and the second syringe 223 through the linkage plate 231, so that the first syringe 222 and the second syringe 223 move synchronously. The piston rod of the second syringe 223 is sealed to the tail end of the outer cylinder of the second syringe 223.

[0093] The POCT blood cell analyzer also includes a housing 10 and a frame 301. The pressure chamber 210 is located on the housing 10 or on the frame 301. The pipette 400, the first syringe 222, the second syringe 223, and the drive unit are located on the frame 301.

[0094] The detection seat 300 is slidably or rotatably positioned relative to the frame 301 and located below the pipette 400.

[0095] The POCT hematology analyzer also includes a metal shielding cover 500, and the test stand 300 can move relative to the frame 301 to the solution preparation station (i.e., Figure 3 The position shown in the figure facilitates the operation of the pipette 400. The detection seat 300 can move relative to the frame 301 to the sample detection station (not shown in the figure, corresponding to directly below the metal shielding cover 500). The metal shielding cover 500 is used to descend at the sample detection station to cooperate with the detection seat 300 to form a relatively closed metal cavity, thereby shielding against external electromagnetic interference. The frame 301 can be configured as a double-layer structure, with the detection seat 300 on the lower layer and the pipette 400, metal shielding cover 500, and syringe 220 on the upper layer.

[0096] like Figure 1 As shown, the housing 10 is equipped with a display screen 11 and an outlet 12. The test holder 300 is used to move to the outlet 12 to receive the reagent kit 20 loaded into the test holder 300. The distance between the solution preparation station and the outlet 12 is less than the distance between the sample detection station and the outlet 12. That is, the outlet 12 faces the instrument interior sequentially as the solution preparation station and the sample detection station. The test holder 300 reciprocates between the loading station, the solution preparation station, and the sample detection station, with the solution preparation station located between the loading station and the sample detection station. In other embodiments, the solution preparation station and the sample detection station can also be the same station.

[0097] This embodiment also provides a method for using the aforementioned POCT blood cell analyzer, including the following steps:

[0098] The drive unit 224 is controlled to move to establish positive or negative pressure on the pressure chamber 210 through the first syringe 222;

[0099] The drive unit 224 is controlled to move to perform pipetting through the second syringe 223 and the pipette 400;

[0100] The pressure release of the control pressure chamber 210 allows the liquid in the front chamber 120 to flow through the micropores to the rear chamber by applying positive pressure to the front chamber 120 or negative pressure to the rear chamber via the pneumatic connection device 510.

[0101] The POCT blood cell analyzer provided in this embodiment has a reasonable layout, compact structure, and is easy to use.

[0102] Please refer to the fourth embodiment as well. Figures 1 to 13 This embodiment provides a reagent kit 20, which includes a box 100 and a microfluidic detection strip 600.

[0103] The housing 100 includes an impedance detection cell (containing a front cell 120, a rear cell, and a pressure chamber 140 that are connected to each other) for impedance detection.

[0104] The microfluidic detection patch 600 is integrally connected to the housing 100, assembled or not connected, and located on the side of the impedance detection cell.

[0105] The microfluidic detection sheet 600 is provided with a sample dispensing hole 601 to receive the sample and lay it flat inside the microfluidic detection sheet 600.

[0106] The box 100 also includes a diluent pool 111, a hemolysin pool 106, an optical detection pool, and a staining lysis pool 602. The diluent pool 111 is used to encapsulate the diluent, the hemolysin pool 106 is used to encapsulate the hemolysin, and the staining lysis pool 602 is used to encapsulate the staining lysis reagent. The optical detection pool is integrally connected to or assembled with the box 100.

[0107] The housing 100 also includes an installation head container and a sample container. The installation head container is used to hold the installation head (tip head 201 or) the puncture head 204, and the sample container is used to hold the sample tube.

[0108] Please refer to the following: Figure 13 This embodiment also provides a POCT blood cell analyzer, which includes a detection base 300, an image recognition base 610, and an image recognition device 620.

[0109] The detection base 300 is equipped with a power supply component (e.g., for impedance detection) to assist in impedance detection. Figure 9 The conductive holder 303 shown in the figure is used to receive the aforementioned reagent kit 20. The image recognition base 610 is located on the side of the detection base 300. The image recognition device 620 is located above the image recognition base 610 and is used to cooperate with the image recognition base 610 to perform image detection on the microfluidic detection sheet 600.

[0110] The POCT blood cell analyzer also includes a pipette 400, which is located above the test base 300. The POCT blood cell analyzer includes a metal shielding cover 500 that matches the test base 300. The metal shielding cover 500 is capable of lifting or rotating. The image recognition device 620 is set independently or connected to the metal shielding cover 500.

[0111] The POCT blood cell analyzer and kit 20 provided in this embodiment can simultaneously support impedance detection and microfluidic image detection, optimizing the efficiency of joint detection and achieving better results.

[0112] Please refer to the fifth embodiment as well. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a frame 301, a test stand 300, and a pipette 400.

[0113] The test stand 300 is slidably or rotatably positioned relative to the frame 301. The test stand 300 reciprocates between the loading station and the sample testing station. When the test stand 300 slides out or rotates out of the frame 301, it is positioned at the loading station to receive the reagent kit 20 for loading or to allow the reagent kit 20 to be removed.

[0114] The pipette 400 is located inside the housing 10 and above the detection seat 300, and is used to perform corresponding operations on the reagent kit 20.

[0115] POCT blood cell analyzers also include a solution preparation station; the solution preparation station is located between the loading station and the sample testing station; or the solution preparation station and the sample testing station are the same station.

[0116] The test socket 300 is a semi-enclosed shape with an opening at the top. The test socket 300 is snapped into the reagent kit 20. The test socket 300 includes a main body 310 and a metal shielding seat (320, 321, 322) covering the outer periphery of the main body 310.

[0117] The POCT hematology analyzer also includes a metal shielding cover 500 that matches the metal shielding base (320, 321, 322). The metal shielding cover 500 is located above the detection base 300 and is configured to move vertically at the sample detection station. The vertical edge of the metal shielding cover 500 abuts against the upper surface of the metal shielding base (320).

[0118] The POCT blood cell analyzer also includes a pressure building system. One end of the pressure building system is provided with a pneumatic connection device 510 that extends into the metal shielding cover 500 and moves synchronously with the metal shielding cover 500. The reagent kit 20 is provided with a pressure chamber 140 that docks with the pneumatic connection device 510.

[0119] This embodiment provides a method for using a POCT blood cell analyzer, which includes the following steps:

[0120] The test stand 300 slides out or rotates out of the frame 301 to receive the reagent kit 20 at the loading station;

[0121] The testing seat 300 slides into or screws into the frame 301 and moves to the liquid preparation station;

[0122] The pipette 400 moves to load the pre-placed mounting tip (tip tip 201 or puncture tip 204) on the reagent kit 20 to perform the corresponding operation to complete the solution preparation;

[0123] The testing station 300 moves from the liquid preparation station to the sample testing station below the metal shielding cover 500;

[0124] The metal shielding cover 500 moves downwards to engage with the detection seat 300;

[0125] The pneumatic connection device 510 connected to the metal shielding cover 500 begins to provide pressure, draining the liquid in the impedance detection cell on the reagent kit 20 for impedance detection.

[0126] The POCT blood cell analyzer and its usage method provided in this embodiment are highly automated. The pretreatment and solution preparation of the test solution are all automatically completed inside the instrument, avoiding the randomness and error of manual operation.

[0127] Please refer to the sixth embodiment as well. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a test base 300 and a metal shielding cover 500.

[0128] The test stand 300 reciprocates between the loading station and the sample testing station. It is used to receive the reagent kit 20, equipped with an impedance detection cell, at the loading station. A metal shielding cover 500 is located at the sample testing station to cover the test stand 300 when it moves to the sample testing station, thus providing electromagnetic signal shielding. The POCT hematology analyzer also includes a solution preparation station; this station is located between the loading station and the sample testing station; or the solution preparation station and the sample testing station may be the same station.

[0129] The detection seat 300 is a semi-enclosed shape with an opening at the top, and the metal shielding cover 500 is a semi-enclosed shape with an opening at the bottom. The detection seat 300 is snapped into place with the reagent kit 20. The detection seat 300 includes a main body 310 and a metal shielding seat (320, 321, 322) covering the outer periphery of the main body 310. The detection seat 300 is configured to be able to translate or rotate under the metal shielding cover 500, and the metal shielding cover 500 is configured to be able to move up and down in the vertical direction.

[0130] The POCT blood cell analyzer also includes a pressure building system. One end of the pressure building system is provided with a pneumatic connection device 510 that extends into the metal shielding cover 500 and moves synchronously with the metal shielding cover 500. The reagent kit 20 is provided with a pressure chamber 140 that docks with the pneumatic connection device 510.

[0131] This embodiment also provides a method for using a POCT blood cell analyzer, which includes the following steps:

[0132] The test stand 300 slides out or rotates out of the frame 301 to receive the reagent kit 20 at the loading station;

[0133] The testing seat 300 slides into or screws into the frame 301 and moves to the liquid preparation station;

[0134] The pipette 400 is used at the solution preparation station to perform the corresponding operations on the reagent kit 20;

[0135] The metal shielding cover 500 and the detection seat 300 are combined to perform impedance testing. Specifically, the detection seat 300 first moves to below the metal shielding cover 500, and then the metal shielding cover 500 moves downward to cover the detection seat 300.

[0136] The process of preparing the reagent kit 20 using pipette 400 includes: pipette 400 drawing blood sample and reagents pre-filled in the reagent kit 20 and transferring them into the detection cell of the reagent kit 20, wherein the reagents include hemolysin and diluent.

[0137] The POCT blood cell analyzer and its usage method provided in this embodiment are highly automated. The pretreatment and solution preparation of the test solution are all automatically completed inside the instrument, avoiding the randomness and error of manual operation.

[0138] Please refer to the seventh embodiment as well. Figures 1 to 13 This embodiment provides a detection seat 300, which includes a detection cavity 302 and a conductive support 303.

[0139] The detection chamber 302 is used to receive the reagent kit 20, which is equipped with an impedance detection cell. The reagent kit 20 is equipped with electrodes, and the conductive holder 303 is used to electrically connect the electrodes. The electrodes are conductive posts 122 located on opposite sides of the reagent kit 20.

[0140] like Figure 10 As shown, in one embodiment, the conductive support 303 includes a downwardly inclined spring portion 304, which supports and electrically connects to the conductive post 122. The conductive support 303 also includes a fixing connection portion 305 integrally connected to the spring portion 304. The shape of the fixing connection portion 305 is not limited, and it may be provided with fixing holes to facilitate fastening assembly by screws.

[0141] like Figure 11 As shown, in another embodiment, the conductive support 303 includes an integrally connected and vertically arranged fixed connection portion 305 and a bent and extended bending portion 306 (which can be a V-shaped bend or an arc-shaped bend). When the conductive post 122 is assembled from top to bottom, the bending portion 306 is pushed open. When the conductive post 122 is assembled in place, the bending portion 306 is reset and abuts against the conductive post 122. This type of conductive support 303 has good elastic deformation capability, is durable, and can maintain a reliable electrical connection for a long time. The conductive support 303 can be a single one and disposed on one side of the conductive post 122, or it can be two and disposed on both sides of the conductive post 122 respectively to further increase the reliability of the electrical connection. Specifically, the bending portion 306 may include a first bending portion 3061 and a second bending portion 3062 sequentially connected to the fixed connection portion 305. When the conductive post 122 is assembled from top to bottom, the first bending portion 3061 is pushed open, and the second bending portion 3062 abuts against the conductive post 122 when the conductive post 122 is assembled in place.

[0142] The detection base 300 also includes a main body 310 and metal shielding bases (320, 321, 322). The main body 310 is provided with a detection cavity 302. The side wall of the main body 310 is provided with an assembly groove 307 communicating with the detection cavity 302. The conductive support 303 extends into the detection cavity 302 through the assembly groove 307. The metal shielding bases (320, 321, 322) cover the outer periphery of the main body 310 and are insulated from the conductive support 303. The metal shielding bases (320, 321, 322) include side plates (320, 321) surrounding the main body 310 and a bottom plate (322) attached to the bottom of the main body 310.

[0143] The reagent kit 20 also includes an integrally connected or detachable optical detection cell. The detachable optical detection cell can be inserted through the rectangular insertion hole 107. The detection base 300 also includes an optical detection component 330 corresponding to the optical detection cell. The optical detection component 330 includes a light-emitting component, a light-receiving component, and a detection cavity located between the light-emitting component and the light-receiving component, which are spaced apart. The angle between the axis of the light-emitting component and the axis of the light-receiving component is in the range of 0 to 60 degrees, specifically 0 degrees, 20 degrees, 30 degrees, 45 degrees, 60 degrees, etc.

[0144] The optical detection components 330 are in multiple groups, and the light source cavities of the multiple optical detection components 330 are spaced apart by light-blocking walls 333 to avoid mutual interference of detection light.

[0145] For example, multiple sets of optical detection components 330 may include two sets of laser detection components 331 and one set of LED light source detection components 332. A light-blocking wall 333 is provided between the detection cavities of the laser detection components 331. The light-blocking wall 333 is preferably blackened to make its light absorption effect better and its reflection effect worse, so as to avoid the reflected light from affecting the normal optical detection.

[0146] The detection base 300 also includes a Peltier 340, a heat sink 350, and a fan 360 arranged sequentially in the detection cavity near the laser detection component 331. The heat sink 350 includes a heat-absorbing substrate 351 attached to the Peltier 340 and heat dissipation fins 352 vertically connected to the heat-absorbing substrate 351. The fan 360 can be located at the end of the heat dissipation fins 352 away from the heat-absorbing substrate 351. By setting the Peltier 340, the heat sink 350, and the fan 360, the detection temperature can be controlled. The detection results obtained by performing the detection at the preset detection temperature are relatively more accurate.

[0147] The bottom of the detection chamber 302 is equipped with a tactile switch 334. The tactile switch 334 can detect the loading status of the reagent kit 20. When the reagent kit 20 is loaded, the tactile switch 334 is pressed down, and when the reagent kit 20 is not loaded, the tactile switch 334 is in the pop-up state.

[0148] This embodiment also provides a POCT blood cell analyzer, which includes a pipette 400 and the aforementioned test seat 300, and the pipette 400 disposed above the test seat 300. The pipette 400 is used to perform corresponding operations on the reagent kit 20.

[0149] The POCT blood cell analyzer and its test base 300 provided in this embodiment have a conductive support 303 with a downwardly inclined spring portion 304, which has good compatibility and stability when electrically connected with the electrodes of the reagent kit 20, greatly reducing the possibility of poor contact.

[0150] Eighth embodiment: This embodiment provides a method for using a POCT blood cell analyzer, including the following steps:

[0151] In response to the reagent kit 20 being placed into the test seat 300, the pipette 400 is controlled to move above the first mounting head reservoir of the reagent kit 20 and descend to load the first mounting head, wherein the first mounting head may be a tip 201;

[0152] Control the pipette 400 to move to the diluent pool 111 of the kit 20 to transfer the diluent into the pre-cell 120 (i.e., the WBC detection cell and / or RBC detection cell) for impedance detection.

[0153] Control the pipette 400 to move to the sample tube / sample dilution chamber / other sample placement area to transfer the sample into the pre-chamber 120, where the sample in the sample dilution chamber is the diluted sample;

[0154] The test solution in the forepool 120 is mixed by aeration or stirring;

[0155] The metal shielding cover 500 is closed with the detection seat 300 to establish a connection between the rear chamber of the reagent kit 20 and the pressure building system.

[0156] The pressure build-up system directs the test solution from the forepool 120 into the rearpool;

[0157] The test solution is tested using impedance detection.

[0158] The step of controlling the pipette 400 to move above the first mounting head container of the reagent kit 20 and descend to load the first mounting head includes: controlling the pipette 400 to move in three dimensions relative to the first mounting head placement container, so that the pipette 400 and the first mounting head are pressed against each other and the first mounting head is loaded.

[0159] Controlling the pipette 400 to move to the dilution pool 111 of the reagent kit 20 to move the dilution pool into the pre-pool 120 for impedance detection and / or controlling the pipette 400 to move to the sample tube / sample dilution pool / other sample placement location to move the sample into the pre-pool 120 includes: the pipette 400 performing three-dimensional movement relative to the pre-pool 120 and using the inner cavity of the first mounting head as a liquid transposition point to aspirate the dilution and / or sample. The volume of the inner cavity of the first mounting head is generally 100 μL to 1000 μL, and is generally not exceeded 4 / 5 of this volume during use to avoid liquid entering the tubing of the pressure build-up system and causing contamination.

[0160] Mixing the test solution in the forepool 120 includes: pipetting the test solution by the pipette 400 through the first mounting head; or pipetting the pipette 400 through the first mounting head to introduce air bubbles into the test solution; or pipetting the pipette 400 through the first mounting head to stir the test solution.

[0161] After the test solution in the forepool 120 is mixed, the pipette 400 is used to unload the first mounting head into the first mounting head receiving pool.

[0162] After unloading the first mounting head into the first mounting head reservoir, the pipette 400 also moves above the second mounting head reservoir and descends to load the second mounting head.

[0163] After the pipette 400 moves to the second mounting head reservoir and descends to load the second mounting head, the following steps are performed:

[0164] The pipette 400 is moved to the sample tube / sample dilution chamber / other sample placement area to transfer the sample into the photodetector.

[0165] The test liquid is detected using optical detection methods.

[0166] After the pipette 400 moves to the second mounting head reservoir and descends to load the second mounting head, the following steps are performed:

[0167] The pipette 400 is moved to the sample tube to transfer the sample into the staining and lysis cell 602 for incubation, and then transferred into the microfluidic detection plate 600;

[0168] Particles in the microfluidic detection sheet 600 are detected using image detection methods.

[0169] This embodiment also provides a POCT blood cell analyzer, which includes a test stand 300, a pipette 400, a pressure building system, a metal shielding cover 500, and a transmission assembly.

[0170] The detection seat 300 is used to receive the reagent kit 20, which is provided with a front chamber 120 and a rear chamber connected by micropores and is equipped with a first mounting head, diluent and sample.

[0171] Pipette 400 is used to load the first mounting head and transfer the diluent and sample into the forepool 120;

[0172] The metal shielding cover 500 is used to cover the detection seat 300, and the pressure building system is used to guide the test liquid from the front pool 120 into the rear pool.

[0173] The transmission assembly is used to drive the detection seat 300, pipette 400 and metal shielding cover 500 to perform one-dimensional, two-dimensional or three-dimensional movements. The transmission assembly can be composed of motors, lead screws, lead nuts, slide rails, sliders, gears, racks, synchronous belts, etc.

[0174] The reagent kit 20 is also equipped with a second mounting head and an optical detection cell. The pipette 400 is also used to load the second mounting head and move the sample into the optical detection cell for optical detection.

[0175] Alternatively, the reagent kit 20 may also be equipped with a second mounting head, staining and lysis reagent, and microfluidic detection strip 600. The POCT hematology analyzer may also include an image detection base 610 and an image detection device 620 spaced apart. The pipette 400 may also be used to load the second mounting head and transfer the sample into the staining and lysis reagent for incubation, then transfer the incubated sample into the microfluidic detection strip 600, and perform image detection on the microfluidic detection strip 600 through the image detection base 610 and the image detection device 620.

[0176] The POCT blood cell analyzer and its usage method provided in this embodiment have a high degree of automation. The automated solution preparation is completed inside the instrument, and the process operation has good consistency. It can simultaneously support impedance detection, optical detection, and image detection.

[0177] Please refer to the ninth embodiment as well. Figures 1 to 13 This embodiment provides a POCT hematology analyzer, which includes a test stand 300, a puncture tip loading mechanism, a puncture tip 204, and a pipette 400. The puncture tip loading mechanism can be a separate mechanism or it can be the same mechanism as the pipette 400.

[0178] The test holder 300 is used to load the reagent kit 20, which is equipped with a diluent pool and a hemolysin pool. The openings of the diluent pool and the hemolysin pool are sealed with a membrane.

[0179] The puncture tip 204 is used to puncture at least two sealing membranes on the reagent kit 20 inserted into the POCT blood cell analyzer one by one, and the puncture tip loading mechanism is used to load the puncture tip 204.

[0180] The puncture tip 204 is disposed on the reagent kit 20 and / or the test port 300. The reagent kit 20 and / or the test port 300 are provided with a placement position that matches the puncture tip 204. After being loaded by the pipette 400, the puncture tip 204 moves synchronously with the pipette 400.

[0181] After the puncture head 204 passes through the sealing membrane, it swings horizontally further, thereby enlarging the diameter of the through hole formed on the sealing membrane.

[0182] Preferably, at least two sealing films are located on the same straight line or the same arc, which makes the action trajectory of piercing one by one relatively simple.

[0183] like Figure 7As shown, the puncture head 204 includes a main body 2042 and a pointed part 2041, with the pointed part 2041 located at one end of the main body 2042. The cross-section of the pointed part 2041 can be in the shape of a straight line, a cross, a star, or a Y. A flange 2044 is provided at the end of the main body 2042 away from the pointed part 2041. The flange 2044 facilitates the mounting of the puncture head 204 on the puncture head receiving pool 104 and can also form a stable abutment fit with the outer sleeve 402.

[0184] Please refer to the tenth embodiment as well. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a test base 300 and a mounting head.

[0185] The test holder 300 is used to load the reagent kit 20. The reagent kit 20 is equipped with a diluent pool and a hemolysin pool. The openings of the diluent pool and the hemolysin pool are sealed with membranes. The mounting head is used to puncture the sealed membranes on the reagent kit 20 loaded into the POCT hematology analyzer one by one. In this embodiment, the mounting head is a tip 201 or a dedicated puncture head 204, which is mounted on the reagent kit 20. The reagent kit 20 has a placement position that matches the mounting head. The outer diameter of the puncture head 204 is larger than the outer diameter of the tip 201. The opening formed by the puncture head 204 after passing through the sealed membrane is larger than the diameter of the tip 201 when it enters the opening. This prevents the formation of a negative pressure chamber in the pool when the tip aspirates liquid, ensuring the accuracy of liquid aspiration.

[0186] The POCT blood cell analyzer also includes a pipette 400, and the mounting head moves with the pipette 400 after being loaded by the pipette 400.

[0187] At least two sealing membranes are located on the same straight line or the same arc. This makes the piercing process relatively simple. After the mounting head passes through the sealing membrane, it swings horizontally to enlarge the diameter of the through holes on the sealing membrane.

[0188] In one embodiment, the tip 201 can first pierce the first through hole with a first stroke, and then pierce the second through hole with a second stroke to perform sample aspiration. The first stroke is shorter than the second stroke, thereby avoiding the formation of a negative pressure sealing cavity during liquid aspiration, which would affect the accuracy of liquid aspiration.

[0189] In another embodiment, the tip is lifted after piercing the through hole before sampling, which can also avoid the formation of a negative pressure sealing cavity during liquid aspiration, thus avoiding affecting the accuracy of liquid aspiration.

[0190] The puncture head 204 includes a main body 2042 and a pointed part 2041 provided at the end of the main body 2042.

[0191] The cross-section of the pointed part 2041 is in the shape of a straight line, a cross, a star, or a Y. A flange part 2044 is provided at the end of the main body part 2042 away from the pointed part 2041.

[0192] In this embodiment, the tip 201 can be used directly for puncture, or a dedicated puncture tip 204 can be used for puncture.

[0193] Please refer to the eleventh embodiment as well. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a housing 10, a pipette 400, and a puncture tip 204.

[0194] The pipette 400 is disposed inside the housing 10. The pipette 400 includes an air tube 401. The puncture head 204 includes a main body 2042 and a sharp part 2041. The sharp part 2041 is disposed at one end of the main body 2042. The other end of the main body 2042 is provided with a receiving cavity 2045 for the air tube 401 to be inserted.

[0195] The pointed end 2041 is used to puncture the sealing film on the reagent kit 20 during automated testing in a POCT hematology analyzer. The cross-section of the pointed end 2041 is in the shape of a straight line, a cross, a star, or a Y. The main body 2042 has a flange 2044 at the end away from the pointed end 2041 to facilitate mounting on the housing 100 of the reagent kit 20. The POCT hematology analyzer also includes a test holder 300 for mounting the reagent kit 20. The reagent kit 20 has a puncture head placement pool, and the flange 2044 supports the outer periphery of the puncture head placement pool.

[0196] The pipette also includes an outer sleeve 402 fitted around the gas delivery tube 401. The lower end of the outer sleeve 402 abuts against the flange 2042. The main body 2042 has multiple ribs 2043 extending toward the sharp point 2041 at one end near the flange 2044 to increase strength. The puncture tip 204 can be integrally molded from metal or plastic.

[0197] like Figure 12 As shown, this embodiment provides a reagent kit 20, which includes multiple pools, one of which is used to place the aforementioned puncture head 204.

[0198] Multiple pools include a diluent pool 111 and / or a hemolysin pool 106, which are equipped with sealing membranes (not shown).

[0199] The cell also includes a WBC detection cell and / or an RBC detection cell for impedance testing.

[0200] like Figure 12As shown, the kit 20 also includes a microfluidic detection sheet 600, which can perform image detection through the image detection base 610 and the image detection device 620.

[0201] In this embodiment, the puncture of the sealing film can be performed without manual operation. A dedicated puncture head 204 or tip head 201 can achieve better consistency.

[0202] Please refer to the twelfth embodiment as well. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a test stand 300 and a pressure build-up component.

[0203] The detection holder 300 is used to mount the reagent kit 20. The reagent kit 20 includes an impedance detection cell for impedance detection. The impedance detection cell includes a front cell 120 and a rear cell connected by micropores. The pressure building component is connected to the front cell 120 and / or the rear cell via a gas path to provide pressure so that the test liquid in the front cell 120 flows through the micropores to the rear cell. It is also used to provide pressure to the front cell 120 or the rear cell before the test liquid is injected into the front cell 120 to ensure that the micropores are unobstructed. When the pressure building component provides negative pressure, impedance detection can be performed. When the pressure building component provides positive pressure, the micropores can be backflushed to ensure the unobstructed flow of the micropores.

[0204] The pressure-building assembly includes a syringe 220 and a pressure chamber 210 connected by an air passage. The syringe 220 is used to establish positive or negative pressure in the pressure chamber 210. The pressure-building assembly is used to provide airflow to mix the test liquid with bubbles or to open the micropores. Specifically, it can be used to open the micropores by blowing or drawing air through the fore chamber 120 / rear chamber.

[0205] The POCT blood cell analyzer includes multiple gate valves, which are connected to pressure chamber 210 and syringe 220.

[0206] The POCT hematology analyzer includes a metal shielding cover 500 that mates with the test base 300. The metal shielding cover 500 is equipped with a pneumatic connection device 510 that communicates with the pressure chamber 210 or the syringe 220. The pneumatic connection device 510 is used to communicate with the rear or front chamber 120. The metal shielding cover 500 is configured to move in the vertical direction.

[0207] The POCT hematology analyzer also includes a pipette 400, which is positioned above the test stand 300 and is used to perform corresponding operations on the reagent kit 20.

[0208] The pipette 400 is connected to the gas path of the pressure building component via a tubing. The pipette 400 is used to load the mounting head on the reagent kit 20 and to perform corresponding operations through the mounting head.

[0209] This embodiment also provides a method for using a POCT blood cell analyzer, which includes the following steps:

[0210] The receiving kit is loaded into the detection seat 300. The kit includes an impedance detection cell for impedance detection, which includes a front cell 120 and a rear cell connected by micropores.

[0211] Before the test solution is injected into the front chamber 120, pressure is applied to the front chamber 120 or the rear chamber to make the micropores open. Specifically, this may include: applying pressure to the front chamber 120 or the rear chamber through the pressure in the pressure chamber 210 of the pressure building component to make the micropores open; or applying pressure to the front chamber 120 or the rear chamber through the syringe 220 of the pressure building component to make the micropores open.

[0212] Please refer to the thirteenth embodiment as well. Figures 1 to 13 This embodiment provides a POCT hematology analyzer, which includes a test stand 300, a human-machine interface module, and a processor. The test stand 300 is used to receive a reagent kit 20, which has at least two test pools. The test stand 300 is equipped with an auxiliary detector for testing the corresponding test pool. The human-machine interface module is communicatively connected to the test stand 300 and is used to select the test. The human-machine interface module is a combination of a touch screen or display screen 11 and mechanical buttons. The processor is signal-connected to the test stand 300 and the human-machine interface module, and is used to receive the test signals from the test and the auxiliary detector, and compare whether the test and the test signals correspond. If they correspond, subsequent operations can proceed normally; if they do not correspond, the correct reagent kit 20 needs to be replaced or the correct test needs to be selected again. The test pools are detachably attached to the reagent kit 20, and the auxiliary detector is used to detect whether the corresponding test pool exists.

[0213] The auxiliary detector includes an optical detection component 330, which includes a light-emitting component and a light-receiving component. The light signal obtained by the light-receiving component is used to help determine whether the item detection pool on the reagent kit 20 corresponds to the selected detection item.

[0214] The auxiliary detector includes a tactile switch 334, and the signal obtained by the tactile switch 334 is used to help determine whether the item detection cell on the kit 20 corresponds to the selected detection item.

[0215] The auxiliary detector includes an image recognition base 610 and an image recognition device 620 arranged at intervals. The signals obtained by the image recognition base 610 and the image recognition device 620 are used to assist in determining whether the item detection pool on the kit 20 corresponds to the selected detection item.

[0216] The POCT blood cell analyzer also includes a barcode scanner, which is used to obtain the label information corresponding to the reagent kit 20. The label information may include information such as name, type, and production time.

[0217] This embodiment also provides a method for using a POCT blood cell analyzer, including the following steps:

[0218] Select the items to be tested in the human-computer interaction module;

[0219] Obtain the corresponding detection bit information from reagent kit 20;

[0220] The processor determines whether the detection bit information corresponds to the selected test item. If they do not correspond, a corresponding prompt is output. If they correspond, subsequent operations can proceed normally. If they do not correspond, the correct reagent kit 20 needs to be replaced or the correct test item needs to be selected again.

[0221] In the step of obtaining the corresponding detection bit information of the reagent kit 20, the corresponding detection bit information of the reagent kit 20 includes scattered light signal, transmitted light signal or electrical signal triggered by micro switch.

[0222] The tests to be performed include at least one of the following: complete blood count, CRP test, SAA test, and blood cell differential test.

[0223] This embodiment also provides a method for using a POCT blood cell analyzer, including:

[0224] The auxiliary detector is used to obtain detection information for items supported by the kit 20;

[0225] Select the items to be tested in the human-computer interaction module;

[0226] Determine whether the detection information of the supported items in the kit 20 corresponds to the selected detection item. If they do not correspond, output the corresponding prompt.

[0227] The solution provided in this embodiment compares the items the user wants to test with the items the kit can actually support before testing, which can effectively prevent errors.

[0228] Please refer to the fourteenth embodiment as well. Figures 1 to 13 This embodiment provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400, a first catheter 211, and an anti-bend component 212.

[0229] The detection holder 300 extends into or out of the housing 10 to receive the reagent kit 20 equipped with an impedance detection cell. The detection holder 300 is equipped with a power supply component (e.g., for impedance detection) to assist in impedance detection. Figure 9 The conductive support 303 shown in the figure.

[0230] The pipette 400 is located inside the housing 10 and above the detection seat 300, and is used to perform corresponding operations on the reagent kit 20.

[0231] One end of the first conduit 211 is connected to the gas path of the pipette 400, and the other end of the first conduit 211 is connected to the pressure build-up system (e.g., ...). Figure 5 The second syringe 223 is connected to the pressure-building system, which may include at least one of the following: syringe 220, pressure chamber 210, solenoid valves (SV1, SV2, SV3), multi-port connector, and manifold. The anti-bending element 212 is combined with the first conduit 211 to prevent excessive bending of the first conduit 211 and blockage of its internal air passage. The inner diameter of the first conduit 211 is relatively small, typically 0.5 mm to 1.5 mm, and is prone to excessive bending and blockage in actual use.

[0232] The anti-bend component 212 can be a sleeve fitted around the outer periphery of the first conduit 211. The hardness of the sleeve is less than or equal to the hardness of the first conduit 211. Of course, the hardness of the sleeve can also be greater than the hardness of the first conduit 211. After the sleeve is fitted around the outer periphery of the first conduit 211, the overall hardness of both increases relatively. The first conduit 211 has a relatively higher hardness, so the deformation of the first conduit 211 itself is relatively smaller when the gas pressure in the first conduit 211 changes, thereby improving the accuracy of sample aspiration. The wall thickness of the sleeve can be greater than or equal to the wall thickness of the first conduit 211, and the bending resistance of the sleeve can be less than or equal to the bending resistance of the first conduit 211. This can reduce the wear of the sleeve on the first conduit 211 and prevent the first conduit 211 from being excessively bent and blocking the internal gas passage of the first conduit 211. The cross-section of the sleeve is annular or C-shaped. The anti-bend component 212 can also be a spring fitted around the outer periphery of the first conduit 211. The anti-folding component 212 can also be a strap that wraps around the outer periphery of the first conduit 211. The strap can be a cloth strap, a plastic strap, or a metal strap, etc.

[0233] The first conduit 211 is connected to a pipette 400 and a second syringe 223 at its two ends, respectively. The end of the first conduit 211 is provided with a connector, which can be located at one or both ends of the first conduit 211. Specifically, the connector can be a clamp, clamp connector, flanged connector, straight connector or threaded connector for gas circuit sealing connection.

[0234] The POCT blood cell analyzer provided in this embodiment has an anti-bend element 212 on the outer periphery of the relatively thin first catheter 211, which can better avoid the occurrence of airway obstruction and improve the stability of the instrument.

[0235] Please refer to the fifteenth embodiment as well. Figures 1 to 13This embodiment provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400, a pressure building system, a first catheter 211, and a second catheter 221.

[0236] The detection holder 300 extends into or out of the housing 10 to receive the reagent kit 20 equipped with an impedance detection cell. The detection holder 300 is equipped with a power supply component (e.g., for impedance detection) to assist in impedance detection. Figure 9 The conductive support 303 shown in the figure.

[0237] The pipette 400 is located inside the housing 10 and above the detection seat 300, and is used to perform corresponding operations on the reagent kit 20.

[0238] The first conduit 211 is connected to a pipette 400 and a second syringe 223 at its two ends, respectively. The second conduit 221 connects the syringe 220, the pressure chamber 210, and the solenoid valves (SV1, SV2, SV3). The first conduit 211 (i.e. Figure 5 The inner diameter of the T3 conduit is less than or equal to that of the second conduit 221 (i.e., Figure 5 The inner diameter of the tubing (excluding T3 and T4) in the diagram shows that the hardness of the first conduit 211 is greater than that of the second conduit 221. The first conduit 211 is mainly used to control the accuracy of sample aspiration or dispensing, requiring minimal error caused by gas compression, hence its high hardness and relatively small inner diameter. The second conduit 221, on the other hand, is mainly used for pressure build-up, requiring rapid pressure build-up, and thus its inner diameter can be relatively larger.

[0239] The ratio of the inner diameter of the first conduit 211 to the inner diameter of the second conduit 221 can be greater than 0.5 and less than or equal to 1. The ratio of the length of the first conduit 211 to the length of the second conduit 221 can be greater than 1 and less than or equal to 1.5. The relatively longer first conduit 211 facilitates the installation of connectors (such as clamp connectors). That is, the first conduit 211 is relatively thinner and rigid, while the second conduit 221 is relatively thicker and softer.

[0240] like Figure 5 As shown, the POCT blood cell analyzer also includes an anti-bend component 212, which is combined with the first catheter 211 to prevent the first catheter 211 from being excessively bent and blocking the internal air passage of the first catheter 211.

[0241] The anti-bending component 212 can be a sleeve or spring fitted around the outer periphery of the first conduit 211. The hardness of the sleeve is less than or equal to the hardness of the first conduit 211. Of course, the hardness of the sleeve can also be greater than the hardness of the first conduit 211. After the sleeve is fitted around the outer periphery of the first conduit 211, the overall hardness of both increases relatively. The first conduit 211 has a relatively greater hardness, so the deformation of the first conduit 211 itself is relatively small when the air pressure in the first conduit 211 changes, thereby improving the accuracy during suction and discharge operations. The wall thickness of the sleeve can be greater than or equal to the wall thickness of the first conduit 211, and the bending resistance of the sleeve can be less than or equal to the bending resistance of the first conduit 211. The cross-section of the sleeve is circular or C-shaped. The anti-bending component 212 can also be a strap wrapped around the outer periphery of the first conduit 211. The strap can be a fabric strap, a plastic strap, or a metal strap.

[0242] The hardness of the first conduit 211 is greater than or equal to the hardness of the second conduit 221. The relatively higher hardness of the first conduit 211 results in less deformation when the gas pressure changes, thus improving sample suction accuracy. The wall thickness of the first conduit 211 is less than or equal to the wall thickness of the second conduit 221. The bending resistance of the first conduit 211 is also less than or equal to that of the second conduit 221. This embodiment selects the inner diameter and hardness according to two different requirements, specifically meeting these needs and achieving precise and efficient instrument operation. Furthermore, an anti-bend component 212 is designed for the thinner first conduit 211, effectively preventing gas path blockage and further improving instrument stability.

[0243] Please refer to the sixteenth embodiment as well. Figures 1 to 14 This embodiment provides a method for using a POCT blood cell analyzer, which may include the following steps:

[0244] S10 receives the power-on trigger signal, specifically, the user can press a mechanical button to turn on the power.

[0245] S11 performs a hardware self-test, which involves detecting and providing feedback on the voltage and current output from each port on the circuit board.

[0246] S12, Determine whether the pipette is loaded with a mounting tip. This can be determined by detection elements such as through-beam optical couplers or reflective optical couplers. The mounting tip can be a tip or a puncture tip.

[0247] S18, if so, the pipette head is unloaded. The unloading location can be a specific location inside the instrument or certain positions on the reagent kit 20 inside the instrument.

[0248] S13, if not, perform overall machine initialization. Initialization means resetting or confirming the zero position of each moving part and clearing the pressure. After initialization, the reagent kit 20 can be loaded and fully automated sample pretreatment (automatic mixing of samples and corresponding reagents) can be performed in the POCT hematology analyzer. Sample testing is performed after automated sample pretreatment is completed.

[0249] The process after unloading the pipette head includes:

[0250] S19, the test socket 300 is removed from the chamber for the reagent kit 20 to be taken out. In this case, it is usually due to an accidental power outage during the last use, and the installation head and reagent kit 20 are still left inside the instrument.

[0251] S20, the test socket 300 is returned to the compartment and the whole machine initialization step is performed again. This step is to ensure that there are no external components (installation head and reagent kit 20) inside the machine, so that the subsequent power-on process will not be affected by the presence of the tip head or reagent kit 20.

[0252] The step of determining whether the pipette is loaded with the mounting head also includes determining whether the test kit 20 is installed in the test socket 300. Generally, if there is an unexpected power outage, both the test kit 20 and the mounting head will be present inside the instrument. Therefore, it is possible to determine whether only the mounting head is inside the instrument, or to determine whether both the mounting head and the test kit 20 are inside the instrument. The test kit 20 can be determined by means of a touch switch inside the test socket 300.

[0253] After the initialization process, the following steps are also included:

[0254] S14, pressure detection is performed to determine whether the pressure in the gas pipeline reaches the threshold range. The POCT blood cell analyzer provided in this application can have one, two, or more pressure chambers 210, and positive or negative pressure is established through a syringe to reach a certain pressure range. Specifically, after initialization, a pressure self-test process is performed. The pressure self-test requires establishing a certain negative pressure, which can be -25 to 30 kPa. The pressure establishment process relies on a solenoid valve, syringe, pressure sensor, pressure chamber 210, and gas pipeline. The pressure establishment process is as follows: During the pressure establishment process, the pressure sensor constantly detects the pressure in pressure chamber 210. First, the syringe motor drives the syringe to pull outward at maximum speed, and at the same time, the solenoid valve is opened to establish negative pressure. At this time, the syringe is directly connected to pressure chamber 210, and there are no other air exchange ports in pressure chamber 210. When the syringe is pulled outward, negative pressure can be established. If the syringe motor reaches its maximum stroke (i.e., the syringe has been pulled to its maximum range) but the target pressure has not been reached, the solenoid valve is closed. At this point, the syringe initializes at maximum speed and distance. After initialization, the solenoid valve is reopened, and the syringe continues to be pulled outwards to build pressure until the target pressure is reached. If the target pressure is not reached by the end of the pressure build-up period, a pressure build-up fault is reported. Pressure build-up is then complete. Pressure self-test continues. Once the target pressure is reached and the pressure sensor detects normal readings, the solenoid valve is opened, connecting the pressure chamber 210 to air and releasing the negative pressure. After the pressure self-test, a simple blank test is performed. Without placing reagent kit 20, the signal under no-load conditions is measured directly to ensure the POCT hematology analyzer's testing performance is normal. The main measurement process then begins.

[0255] S15, perform signal detection, including judging the detection signal after the electromagnetic shielding components are combined to judge the electromagnetic shielding effect, and judging whether the current and voltage signals are within the threshold range. The signal detection can include various photoelectric signals, and also includes simulating impedance detection after the metal shielding cover and metal shielding base are combined and sealed. Based on the output results, judge the shielding effect of the metal shielding cover and metal shielding base or the signal quality of the circuit board itself.

[0256] S16, enter standby mode. At this time, you can choose to power off or perform a test.

[0257] Specifically, the steps to enter standby mode include:

[0258] S17 receives a power-off trigger signal, i.e., when the user presses the power-off button. To prevent accidental operation, it can be set so that after the user presses the power-off button, the screen displays whether the user is sure about powering off.

[0259] S21, The presence or absence of reagent kit 20 can be determined by a touch switch inside the test socket 300;

[0260] S22, if reagent kit 20 is determined to be in a certain condition, further determination of the door status may be necessary;

[0261] S24, if it is determined that the door is open, then prompt to remove reagent kit 20 and return to the step of determining whether reagent kit 20 exists;

[0262] S23, if it is determined that the door is closed, open the door and prompt the user to take out reagent kit 20 before returning to the step of determining whether reagent kit 20 is present;

[0263] S25, if reagent kit 20 is determined to be absent, further determine the door status;

[0264] S26, If it is determined that the compartment door is open, close the compartment door and prompt the user to turn off the power;

[0265] S27, if it is determined that the compartment door is closed, then prompt to turn off the power.

[0266] The steps to enter standby mode include:

[0267] S31, receive sample ID settings and measurement mode settings. Specifically, the screen can provide options such as mode settings, and offers some optional items for the user to choose from, such as CRP test, SAA test, blood test, etc.

[0268] S32 receives the start test trigger signal, that is, a touch button or physical button can be provided on the screen or instrument to generate the start test trigger signal;

[0269] S33, a pop-up window prompts you to check whether the test information (such as reagent information, or QR code comparison / IC card reading comparison) matches the measurement mode. If they do not match, click Cancel (S46) and return to step S31 to receive the sample ID setting and measurement mode setting.

[0270] S34, if it matches, click OK;

[0271] S35, automatically opens the cargo door;

[0272] S36 can prompt you to insert the reagent kit via pop-up windows or other means;

[0273] S37, after inserting the reagent kit, click OK;

[0274] S38, check if kit 20 is present. If not, return to step S36 and prompt to insert kit 20.

[0275] S39, if present, automatically closes the compartment door;

[0276] S40, Perform automated solution preparation test. The specific process for automated solution preparation test can be referred to the aforementioned embodiments.

[0277] S41 displays the test results, which can be displayed on a screen or printed out using a printing device.

[0278] S42, click OK;

[0279] S43, automatically opens the compartment door and prompts you to remove reagent kit 20;

[0280] S44, After the user retrieves reagent kit 20, click OK;

[0281] S45 automatically closes the compartment door and enters standby mode.

[0282] The POCT blood cell analyzer provided in this embodiment has a reasonable and orderly usage method, which can quickly perform single-item testing or simultaneous testing of multiple items.

[0283] This embodiment also provides a POCT blood cell analyzer, including a housing 10, a test stand 300, and a pipette 400.

[0284] The detection seat 300 can extend into or out of the housing 10 to receive the reagent kit 20 and reciprocate between the reagent kit 20 loading station and the sample detection station; the pipette 400 is located inside the housing 10 and above the detection seat 300 for automated sample pretreatment of the reagent kit 20.

[0285] The kit 20 includes a front chamber 120 and a rear chamber connected by micropores. The POCT hematology analyzer also includes a pressure building system located inside the housing 10 and used to allow the test solution to flow from the front chamber 120 to the rear chamber for impedance detection.

[0286] The POCT hematology analyzer also includes a metal shield (320, 321, 322) for fitting with the test seat 300. The reagent kit 20 includes a pressure chamber 140 communicating with the rear chamber. The metal shield (320, 321, 322) is provided with a pneumatic connection device 510 for connecting with the pressure building system. When the metal shield (320, 321, 322) fits with the test seat 300, the pneumatic connection device 510 and the pressure chamber 140 are sealed together.

[0287] The pipette 400 is equipped with a detection element (e.g.) Figure 6 The optical coupler 405 shown, the pipette 400 includes an air delivery tube 401 and an outer sleeve 402 sleeved on the outer periphery of the air delivery tube 401. The air delivery tube 401 is used to load the mounting head mounted on the reagent kit 20. When the mounting head is loaded on the air delivery tube 401, the air delivery tube 401 is inserted into the mounting head, so that the mounting head pushes the outer sleeve 402 to change position along the axial direction of the air delivery tube 401. The detection element is used to detect the position of the outer sleeve 402 and thus determine whether the mounting head is sleeved on the air delivery tube 401.

[0288] The pipette 400 also includes a head retraction mechanism (refer to the aforementioned stop 4031), which is used to stop the outer tube 402 from rising as the gas delivery tube 401 rises so that the outer tube 402 unloads the mounting head.

[0289] The head-retracting mechanism is an electromagnet, motor, or U-shaped baffle arranged axially or radially relative to the air guide tube 401.

[0290] The pressure building system includes a pressure chamber 210, a first syringe 222 and a second syringe 223 that are linked together. The first syringe 222 is used to establish positive or negative pressure in the pressure chamber 210. The second syringe 223 is connected to the pipette 400 and is used to use the inner cavity of the mounting head as a liquid transfer device to draw reagents and / or samples when the pipette 400 is fitted with a mounting head.

[0291] The POCT blood cell analyzer includes a first catheter 211 and a second catheter 221. The first catheter 211 is connected between the second syringe 223 and the pipette 400. The second catheter 221 is used to connect the syringe and the pressure chamber 210. The inner diameter of the first catheter 211 is smaller than the inner diameter of the second catheter 221.

[0292] The outer periphery of the first conduit 211 is provided with an anti-bending element 212, which is a sleeve, spring or strap.

[0293] This embodiment also provides a POCT blood cell analyzer, which includes a test stand 300, a pipette 400 disposed above the test stand 300, and a pressure building system. The test stand 300 is used to receive the reagent kit 20. The reagent kit 20 is provided with a front chamber 120 and a rear chamber connected by micropores and is equipped with reagents, samples, and mounting heads. The pressure building system includes a pressure chamber 210, a pneumatic connection device 510, a first syringe 222, a second syringe 223, a drive component 224, a first solenoid valve SV1, a second solenoid valve SV2, and a third solenoid valve SV3.

[0294] The pneumatic connection device 510 is connected to the pressure chamber 210 and is used to apply positive or negative pressure to the front chamber 120 or the rear chamber to allow the liquid in the front chamber 120 to flow to the rear chamber; the first syringe 222 is connected to the pressure chamber 210 / pipette 400 and is used to establish positive or negative pressure in the pressure chamber 210, and also to assist the pipette 400 in pipetting, or to aerate and mix the liquid in the front chamber 120; the second syringe 223 is connected to the pipette 400 for pipetting; the driving component is used to simultaneously drive the first syringe 222 and the second syringe 223; the first solenoid valve SV1 selectively connects the first syringe 222 to the pressure chamber 210 and the second syringe 223; the second solenoid valve SV2 selectively connects the first solenoid valve to the pressure chamber 210 and the outside atmosphere; the third solenoid valve SV3 selectively connects the pneumatic connection device to the pressure chamber 210.

[0295] This application also provides a reagent kit 20, which includes a box body 100, a tip head reservoir (101, 102, 103), an mounting head reservoir, a sample reservoir 105, a hemolysin reservoir 106, a rectangular insertion hole 107, a circular insertion hole 108, a front reservoir 120, a mounting cavity, a mounting base 160, a microplate 170, a front reservoir electrode 121, a rear reservoir electrode 165, a pressure chamber 140, tip heads (201, 202, 203), a puncture head 204, a sample tube 205, a hemolysin container 206, a first reservoir body 207, a second reservoir body 208, etc.

[0296] The following will provide specific examples of various embodiments of kit 20.

[0297] Please refer to the seventeenth embodiment as well. Figure 15 and Figure 20 This application provides a microporous sheet 170, which includes a sheet body 171. The sheet body 171 is provided with micropores 172 that allow cells to pass through one by one. The micropores 172 can be made in different specifications according to cells of different sizes. The sheet body 171 is a plastic sheet or a ceramic sheet. The mechanical strength of the plastic sheet or ceramic sheet is relatively weak. This application further provides a reinforcing part 173 on the sheet body 171 to ensure the mechanical strength of the microporous sheet 170, while also having the effect of convenient installation. When the microporous sheet 170 is installed, the micropores 172 are not easily contaminated or worn by contact. The material cost of the plastic sheet or ceramic sheet is relatively cheap, and it can be used as a disposable product without the need to use expensive materials that can be repeatedly cleaned and reused.

[0298] The sheet 171 has a first surface 175 and a second surface 176 facing away from each other, and the first surface 175 and / or the second surface 176 are provided with a reinforcing portion 173.

[0299] The reinforcing part 173 may be disposed near the edge of the sheet body 171. The reinforcing part 173 may be a structure such as a convex ring. The convex ring may be a continuous integral convex ring or a convex ring composed of multiple convex rings. The outer edge of the convex ring may or may not coincide with the outer edge of the sheet body 171.

[0300] The convex ring is connected to the first surface 175 and / or the second surface 176 of the sheet 171 via a vertical surface, a slope, or an arc surface, wherein the slope or arc surface can further reduce sample residue and improve detection accuracy.

[0301] The first surface 175 of the sheet body 171 is provided with a recessed drainage portion 174 around the micropore 172. The recessed drainage portion 174 may be spherical or conical.

[0302] The ratio of the thickness of the convex ring to the thickness of the sheet 171 is 0.2-2. Preferably, the ratio of the width of the convex ring to the radius of the sheet 171 is less than or equal to 1. If the thickness of the convex ring is too small, it will not provide adequate mechanical strength reinforcement; if the thickness is too large, it will waste material. Furthermore, if the thickness of the convex ring is too large, the wall thickness of the micropores 172 of the microporous sheet 170 will be difficult to control, resulting in a more complex manufacturing process. Similarly, the ratio of the width of the convex ring to the radius of the sheet 171 is 0.2-0.8. Likewise, if the width of the convex ring is too small, it will not provide adequate mechanical strength reinforcement; if the width of the convex ring is too large, the wall thickness of the micropores 172 of the microporous sheet 170 will also be difficult to control, resulting in a more complex manufacturing process. If the width of the convex ring is too large (e.g., close to the radius of the sheet 171), it will also lengthen the axial channel of the micropores 172, further causing backflow of sample particles and affecting the accuracy of cell detection.

[0303] The microporous sheet 170 provided in this embodiment can improve the mechanical strength of the microporous sheet 170 by providing the reinforcing part 173, and prevent the microporous sheet 170 made of plastic or ceramic materials from easily deforming during assembly. This embodiment also provides a reagent kit 20 including the aforementioned microporous sheet 170. The specific structure of the reagent kit 20 is described below.

[0304] Please refer to the eighteenth embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a housing 100, a mounting base 160, and a microplate 170.

[0305] The housing 100 includes a pre-cell 120, and the housing 100 is provided with a pre-cell 120 electrode 121 corresponding to the pre-cell 120. The pre-cell 120 can be set in two sets, which are used to cooperate in the detection of WBC (white blood cells) and RBC (red blood cells).

[0306] like Figure 18As shown, the mounting base 160 is connected to the housing 100. The mounting base 160 is provided with an axial drainage cavity 167 (the axial drainage cavity 167 can also be called the rear pool) and a rear pool electrode 165 extending into the axial drainage cavity 167.

[0307] like Figure 1 , Figure 2 , Figure 18 As shown, the microporous sheet 170 is provided with micropores 172 that allow cells to pass through one by one. The microporous sheet 170 is located between the anterior chamber 120 and the axial drainage cavity 167. The anterior chamber 120 and the axial drainage cavity 167 are connected through the micropores 172. The anterior chamber 120 electrode 121 and the posterior chamber electrode 165 are respectively located on both sides of the microporous sheet 170 at intervals.

[0308] The mounting base 160 is detachably connected to the housing 100.

[0309] In a specific embodiment, the front electrode 121 of the front chamber 120 can be integrally injection molded with the housing 100 or detachably connected. The rear electrode 165 can be integrally injection molded with the mounting base 160 or detachably connected. The front electrode 121 and / or the rear electrode 165 are columnar electrodes, and the length of the rear electrode 165 is greater than or equal to the length of the front electrode 121. The microporous plate 170 can be integrally injection molded with the housing 100 or detachably connected, or the microporous plate 170 can be integrally injection molded with the mounting base 160 or detachably connected.

[0310] The housing 100 is provided with a mounting cavity 130, and the mounting base 160 is engaged with the mounting cavity 130 by snap-fit, threaded fit, interference fit, laser welding fit, or adhesive fit.

[0311] The housing 100 and / or mounting base 160 are made of plastic. The front cell 120 electrode 121 is embedded in the housing 100 and is flush with, protruding or recessed on the outer surface of the housing 100. The rear cell electrode 165 is embedded in the mounting base 160 and is flush with, protruding or recessed on the outer end face of the mounting base 160. The outer ends of the front cell 120 electrode 121 and the rear cell electrode 165 (i.e. the two ends that are far apart) are used to connect the working voltage. The inner ends of the front cell 120 electrode 121 and the rear cell electrode 165 (i.e. the two ends that are close to each other) are in contact with the sample liquid to be tested. The axial drainage cavity 167 will be filled with the sample liquid to be tested during detection.

[0312] The kit 20 also includes an inner sealing ring 164, which is disposed between the microwell plate 170 and the fore chamber 120. Specifically, it can be disposed between the first surface 175 of the microwell plate 170 and the fore chamber 120, so that the sample solution to be tested in the fore chamber 120 can only enter the axial drainage cavity 167 through the microwell 172.

[0313] The kit 20 also includes an outer sealing ring 166, which is disposed between the assembly base 160 and the free end of the mounting cavity 130.

[0314] In one embodiment, the inner sealing ring 164, the outer sealing ring 166, the microporous plate 170, and the mounting base 160 are separate structural components.

[0315] In another embodiment, the inner sealing ring 164, the outer sealing ring 166, the microporous plate 170, and the mounting base 160 can be an integral structural component to reduce the number of assembly parts, reduce assembly difficulty, and save assembly time. The inner sealing ring 164 and the outer sealing ring 166 can be injection molded using a secondary injection molding process with relatively soft plastic materials.

[0316] In another embodiment, the inner sealing ring 164, the outer sealing ring 166, and the mounting base 160 are an integral structural component. The microporous plate 170 is detachably connected to this integral structural component, which reduces the number of assembly parts while ensuring the manufacturing precision and yield of the microporous plate 170. The inner sealing ring 164 has a certain degree of flexibility. When the microporous plate 170 is installed with the integral structural component, it can be installed behind the inner sealing ring 164 due to its flexibility, while the inner sealing ring 164 still seals the first surface 175 of the microporous plate 170.

[0317] The reagent kit 20 provided in this embodiment has a novel structure and is easy to assemble.

[0318] Please refer to the nineteenth embodiment as well. Figure 15 and Figure 20 This application provides an assembly base 160, which includes an assembly cylinder 168 and a rear electrode 165.

[0319] Assembly cylinder 168 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611 that are interconnected (see...) Figure 16 The radial discharge groove 1611 is used to discharge gas or liquid from the axial drainage cavity 167; the rear pool electrode 165 is connected to the assembly cylinder 168 and extends into the axial drainage cavity 167.

[0320] The first preset distance is between the inner end of the radial outlet groove 1611 and the inner end of the assembly cylinder 168, and the second preset distance is between the inner end of the rear electrode 165 and the inner end of the assembly cylinder 168. The first preset distance is less than or equal to the second preset distance. When the first preset distance is less than the second preset distance, air bubbles in the axial drainage cavity 167 are more easily discharged from the axial drainage cavity 167. If air bubbles remain in the axial drainage cavity 167, it will affect the detection accuracy. Here, the inner end is with the inside of the box 100 as a reference. When the front pool 120 is used as a reference, the end pointing towards the front pool 120 is the inner end, and the end away from the front pool 120 is the outer end. For example, the inner end of the mounting cavity 130 is connected to the front pool 120 through the through hole 132, and the outer end of the mounting cavity 130 is an open end for receiving the mounting base 160.

[0321] The radial outlet groove 1611 has an axial length in the axial drainage cavity 167 that is greater than or equal to the length of the rear chamber electrode 165 extending into the axial drainage cavity 167. The inner end of the rear chamber electrode 165 may or may not extend into the axial drainage cavity 167; that is, the inner end of the rear chamber electrode 165 may protrude, be flush with, or be recessed into the bottom surface of the axial drainage cavity 167.

[0322] Furthermore, the outer surface of the assembly cylinder 168 is provided with a recessed area communicating with the radial liquid outlet groove 1611 to form a guide groove 1612. The guide groove 1612 allows gas or liquid in the axial drainage cavity 167 to exit through the radial liquid outlet groove 1611 directly above and then flow away through the guide groove 1612 obliquely above. Thus, the two assembly seats 160 can share a pressure action chamber 140 communicating with the two assembly seats 160. The pressure action chamber 140 is connected through a through hole 133 (which can be...). Figure 17 The fan-shaped section shown is connected to the mounting cavity 130, and further connected to the axial drainage cavity 167 via the guide groove 1612, the radial liquid outlet groove 1611, and the axial drainage cavity 167 in sequence.

[0323] The inner end of the assembly tube 168 is provided with a microporous sheet 170 that allows cells to pass through one by one. The specific structure of the microporous sheet 170 can be referred to the aforementioned embodiment.

[0324] The distance from the inner end of the rear pool electrode 165 to the microporous plate 170 is a third preset distance, which is 0.2-2 times the axial length of the axial drainage cavity 167. Experiments have verified that within this range, better signal accuracy can be obtained when performing impedance detection.

[0325] The inner end of the assembly cylinder 168 is provided with a recessed platform 1613, and the micro-perforated plate 170 is connected to the recessed platform 1613. The reinforcing part 173 of the micro-perforated plate 170 abuts against the recessed platform 1613.

[0326] like Figure 16As shown, the assembly cylinder 168 includes an integrally connected rear tank cylinder 161, end plate 162 and outer cylinder 163. The rear tank cylinder 161 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611. The end plate 162 radially connects the rear tank cylinder 161 and the outer cylinder 163. The outer cylinder 163 is spaced around the outer periphery of the rear tank cylinder 161.

[0327] The radial liquid outlet groove 1611 can be in the shape of a round hole, a waist-shaped hole, or a rectangular hole.

[0328] This application also provides a reagent kit 20, which includes a box body 100 and the aforementioned mounting base 160. The box body 100 is provided with a front pool 120 and a mounting cavity 130 that are connected. The mounting cylinder 168 is connected to the mounting cavity 130. The front pool 120 and the axial drainage cavity 167 are connected through the micropores 172 of the microporous sheet 170.

[0329] The reagent kit 20 and its assembly base 160 provided in this embodiment limit the distance between the inner end of the radial liquid outlet groove 1611 and the inner end of the rear cell electrode 165 and the inner end of the assembly cylinder 168, which facilitates the discharge of air bubbles in the assembly cylinder 168 and avoids air bubbles remaining in the assembly cylinder 168 from affecting the detection accuracy of impedance detection.

[0330] Please refer to the twentieth embodiment as well. Figure 15 and Figure 20 This application provides an assembly base 160, which includes an assembly cylinder 168 and a rear electrode 165. The rear electrode 165 is a columnar electrode and is connected to the assembly cylinder 168.

[0331] The assembly cylinder 168 is provided with a radial liquid outlet groove 1611 and a fastening part 1631. The fastening part 1631 is aligned with the radial liquid outlet groove 1611. The fastening part 1631 can be a fastening hole or a fastening protrusion. Correspondingly, the outer surface of the mounting cavity 130 is provided with a snap block 131 corresponding to the fastening hole. The fact that the opening direction of one of the fastening parts 1631 and the radial liquid outlet groove 1611 is the same and they are aligned can make it convenient to demold from the same direction during mold injection molding.

[0332] The assembly cylinder 168 is provided with an axial drainage cavity 167, which is connected to the radial liquid outlet groove 1611, and the rear pool electrode 165 extends into the axial drainage cavity 167.

[0333] The rear electrode 165 can be embedded in the mounting base 160 by injection molding and be flush with, protruding or recessed on the outer end face of the mounting base 160.

[0334] In this embodiment of the application, the assembly cylinder 168 includes an integrally connected rear tank cylinder 161, end plate 162 and outer cylinder 163. The rear tank cylinder 161 is provided with a radial liquid outlet groove 1611 and an axial drainage cavity 167. The end plate 162 radially connects the rear tank cylinder 161 and the outer cylinder 163.

[0335] The outer cylinder 163 can extend along the axial direction with a plurality of spaced positioning protrusions 1632, and the fastening part 1631 is provided corresponding to the positioning protrusions 1632.

[0336] The distance between the inner end of the radial liquid outlet 1611 and the inner end of the assembly cylinder 168 is a first preset distance, and the distance between the inner end of the rear electrode 165 and the inner end of the assembly cylinder 168 is a second preset distance. The first preset distance is less than or equal to the second preset distance.

[0337] The rear electrode 165 and the assembly cylinder 168 can be integrally formed or detachably connected.

[0338] The inner end of the rear pool cylinder 161 is provided with a platform 1613 for assembling a microporous sheet 170 that allows cells to pass through one by one.

[0339] This application also provides a reagent kit 20, which includes a box body 100 and the aforementioned mounting base 160. The box body 100 is provided with a front pool 120 and a mounting cavity 130 that are connected. The mounting cylinder 168 of the mounting base 160 is connected to the mounting cavity 130. The front pool 120 and the axial drainage cavity 167 are connected through the micropores 172 of the microporous sheet 170.

[0340] The reagent kit 20 and its mounting base 160 provided in this embodiment are aligned with the radial liquid outlet groove 1611 and the fastening part 1631, which facilitates the demolding operation after the mold is formed.

[0341] Please refer to the twenty-first embodiment as well. Figure 15 and Figure 20 This application provides an assembly base 160, which includes an assembly cylinder 168 and a rear electrode 165.

[0342] The assembly cylinder 168 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611 that are interconnected. The outer surface of the assembly cylinder 168 is provided with a recessed area that communicates with the radial liquid outlet groove 1611 to form a guide groove 1612. The guide groove 1612 allows gas or liquid in the axial drainage cavity 167 to exit through (e.g., directly above) the radial liquid outlet groove 1611 and then flow smoothly through (e.g., obliquely above) the guide groove 1612 to the pressure action cavity 140. Thus, the two assembly seats 160 can share a pressure action cavity 140 that is connected to both assembly seats 160. The pressure action cavity 140 is connected through a through hole 133 (which can be...). Figure 17The fan-shaped section shown is connected to the mounting cavity 130, and further connected to the axial drainage cavity 167 via the guide groove 1612, the radial liquid outlet groove 1611, and the axial drainage cavity 167 in sequence.

[0343] The rear pool electrode 165 is connected to the assembly cylinder 168 and extends into the axial drainage cavity 167.

[0344] The flow guide 1612 is circumferentially arranged along the outer surface of the assembly cylinder 168. The flow guide 1612 has an arc-shaped structure with a central angle of 0° to 360°, which allows the sample liquid to flow more smoothly into the pressure chamber 140. The recess depth of the flow guide 1612 is 1 / 5 to 4 / 5 of the wall thickness of the assembly cylinder 168, ensuring smooth liquid flow while also providing sufficient mechanical strength to prevent damage.

[0345] The radial liquid outlet groove 1611 and the guide groove 1612 are connected by a transition surface, which can be a vertical plane, an inclined surface or an arc surface.

[0346] The rear electrode 165 is embedded in the mounting base 160 and is flush with, protruding from or recessed in the outer end face of the mounting base 160.

[0347] The distance between the inner end of the radial outlet groove 1611 and the inner end of the assembly cylinder 168 is a first preset distance; the distance between the inner end of the rear electrode 165 and the inner end of the assembly cylinder 168 is a second preset distance; the first preset distance is less than or equal to the second preset distance.

[0348] like Figure 16 As shown, the assembly cylinder 168 includes an integrally connected rear tank cylinder 161, end plate 162 and outer cylinder 163. The rear tank cylinder 161 is provided with a radial liquid outlet groove 1611 and an axial drainage cavity 167. The end plate 162 radially connects the rear tank cylinder 161 and the outer cylinder 163.

[0349] The outer cylinder 163 extends axially with a plurality of spaced positioning protrusions 1632, and the fastening part 1631 is provided corresponding to the positioning protrusions 1632.

[0350] This application also provides a reagent kit 20, which includes a box body 100 and the aforementioned mounting base 160. The box body 100 is provided with a front pool 120 and a mounting cavity 130 that are connected to each other. The mounting cylinder 168 of the mounting base 160 is connected to the mounting cavity 130, and the front pool 120 is connected to the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170.

[0351] The reagent kit 20 and its assembly base 160 provided in this embodiment can facilitate the sharing of a pressure chamber 140 that is connected to the two assembly bases 160 by setting a radial liquid outlet groove 1611 on the assembly cylinder 168 and forming a recessed area on the outer surface of the radial liquid outlet groove 1611 to form a guide groove 1612. This makes it convenient for the two assembly bases 160 to perform negative pressure drainage.

[0352] Please refer to the twenty-second embodiment as well. Figure 15 and Figure 20 This application provides an assembly base 160, which includes an assembly cylinder 168 and a rear electrode 165.

[0353] The assembly cylinder 168 is a plastic assembly cylinder with an axial drainage cavity 167 and a radial liquid outlet groove 1611 that are connected to each other. The radial liquid outlet groove 1611 is used to discharge gas or liquid in the axial drainage cavity 167. The rear pool electrode 165 is connected to the assembly cylinder 168 and extends into the axial drainage cavity 167.

[0354] The outer surface of the assembly cylinder 168 is provided with a recessed area communicating with the radial liquid outlet groove 1611 to form a guide groove 1612. The guide groove 1612 allows gas or liquid in the axial drainage cavity 167 to flow smoothly to the pressure action cavity 140 after exiting through the radial liquid outlet groove 1611 (e.g., directly above) and then through the guide groove 1612 (e.g., obliquely above). Thus, the two assembly seats 160 can share a pressure action cavity 140 communicating with the two assembly seats 160. The pressure action cavity 140 is connected through the through hole 133 (which can be...). Figure 17 The fan-shaped section shown is connected to the mounting cavity 130, and further connected to the axial drainage cavity 167 via the guide groove 1612, the radial liquid outlet groove 1611, and the axial drainage cavity 167 in sequence.

[0355] The assembly cylinder 168 is provided with a fastening part 1631, which is aligned with the radial liquid outlet groove 1611. The fastening part 1631 can be a fastening hole. The alignment of the fastening part 1631 and the radial liquid outlet groove 1611 makes it convenient to remove the mold from the same direction during injection molding.

[0356] The distance between the inner end of the radial outlet groove 1611 and the inner end of the assembly cylinder 168 is a first preset distance; the distance between the inner end of the rear electrode 165 and the inner end of the assembly cylinder 168 is a second preset distance; the first preset distance is less than or equal to the second preset distance. When the first preset distance is less than the second preset distance, air bubbles in the axial drainage cavity 167 are more easily discharged from the axial drainage cavity 167. If air bubbles remain in the axial drainage cavity 167, it will affect the detection accuracy.

[0357] The axial length of the radial outlet groove 1611 in the axial drainage cavity 167 is greater than or equal to the length of the rear pool electrode 165 extending into the axial drainage cavity 167.

[0358] The inner end of the assembly cylinder 168 is provided with a microporous plate 170 that allows cells to pass through one by one. The distance from the inner end of the rear chamber electrode 165 to the microporous plate 170 is a third preset distance, which is 0.2-2 times the axial length of the axial drainage cavity 167. The inner end of the assembly cylinder 168 is provided with a countersunk platform 1613, and the microporous plate 170 is connected to the countersunk platform 1613.

[0359] In this embodiment of the application, the assembly cylinder 168 includes an integrally connected rear tank cylinder 161, an end plate 162, and an outer cylinder 163. The rear tank cylinder 161 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611. The end plate 162 radially connects the rear tank cylinder 161 and the outer cylinder 163. The outer cylinder 163 is spaced around the outer periphery of the rear tank cylinder 161, and a fastening part 1631 is provided on the outer cylinder 163.

[0360] This application also provides a reagent kit 20, which includes a box body 100 and the aforementioned mounting base 160. The box body 100 is provided with a front pool 120 and a mounting cavity 130 that are connected to each other. The mounting cylinder 168 of the mounting base 160 is connected to the mounting cavity 130. The front pool 120 and the axial drainage cavity 167 are connected through the micropores 172 of the microporous sheet 170. The front pool 120, the mounting cavity 130, the axial drainage cavity 167, the radial liquid outlet groove 1611, and the pressure action cavity 140 together constitute a drainage channel. The axial drainage cavity 167 can receive negative pressure to draw the sample to be tested in the front pool 120 through the micropores 172 of the microporous sheet 170.

[0361] The reagent kit 20 and its mounting base 160 provided in this embodiment have a novel structure and are easy to manufacture and assemble.

[0362] Please refer to the twenty-third embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a box body 100, a hemolysin pool 106 on the box body 100, and a diluent pool 111 near the hemolysin pool 106.

[0363] At least two of the hemolytic agent pool 106, the diluent pool 111, and the box body 100 are integrally connected, and at least two of the hemolytic agent pool 106, the diluent pool 111, and the box body 100 are detachably connected. That is to say, the box body 100, the hemolytic agent pool 106, and the diluent pool 111 can be a single-piece structure or any separate structure.

[0364] For example, the box body 100, the hemolysin pool 106, and the diluent pool 111 are three independent components that can be detachably connected to each other; or, the diluent pool 111 and the box body 100 are an integral structural component, and a separate hemolysin container 206 is detachably assembled to the hemolysin pool 106; or, the hemolysin pool 106 and the box body 106 are an integral structural component, and a separate diluent pool test tube is detachably assembled to the diluent pool 111; or, the hemolysin pool 106 and the diluent pool 111 are connected as one unit and are detachably connected to the box body 100.

[0365] Any two of the hemolytic agent pool 106, diluent pool 111, and housing 100 form an assembly. The assembly is provided with an assembly part (e.g., the inner peripheral wall of the insertion hole provided on the housing 100). The other of the three pools (hemolytic agent pool 106, diluent pool 111, and housing 100) is provided with a disassembly part (e.g., ...). Figure 15 The outer peripheral wall of the hemolytic agent container 206 shown in the figure is connected to the assembly part by means of disassembly.

[0366] The surface of the assembly or disassembly part is further provided with a protruding structure 2061, which allows the hemolytic agent container 206 to abut against the insertion hole provided on the box body 100.

[0367] In one embodiment, the housing 100 is provided with two assembly positions, and the hemolytic agent pool 106 and the diluent pool 111 are respectively installed in the two assembly positions.

[0368] In this embodiment of the application, the housing 100 includes a front pool 120 and a mounting cavity 130 that are connected to each other. The front pool 120 and the mounting cavity 130 are connected by a through hole 132 (see...). Figure 17 and Figure 18 The front chamber 120 is equipped with a front chamber 120 electrode 121. The reagent kit 20 also includes an assembly base 160 connected to the mounting cavity 130. The assembly base 160 is provided with a microporous plate 170 and a rear chamber electrode 165. The microporous plate 170 is installed at the through hole 132. The front chamber 120 electrode 121 and the rear chamber electrode 165 are respectively located on both sides of the microporous plate 170. The assembly base 160 includes an assembly cylinder 168, which is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611.

[0369] In this embodiment of the application, the assembly cylinder 168 includes an integrally connected rear tank cylinder 161, end plate 162 and outer cylinder 163. The rear tank cylinder 161 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611. The end plate 162 radially connects the rear tank cylinder 161 and the outer cylinder 163. The outer cylinder 163 is spaced around the outer periphery of the rear tank cylinder 161.

[0370] The amount of diluent used in the diluent pool 111 is generally relatively large. Therefore, in this embodiment, the volume of the diluent pool 111 is greater than or equal to the volume of the hemolysin pool 106.

[0371] The openings of the diluent pool 111 and the hemolysin pool 106 are sealed with membranes to facilitate long-term preservation of the diluent and hemolysin.

[0372] The reagent kit 20 provided in this embodiment has a novel structure and multiple detachable options, which can adapt to a variety of different detection needs.

[0373] Please refer to the twenty-fourth embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a housing 100 and at least one detection pool. The at least one detection pool is integrally connected to the housing 100 or detachably connected to it.

[0374] The project testing pool includes a first project testing pool for performing a first project test and a second project testing pool for performing a second project test. The first project testing pool and / or the second project testing pool are integrally connected to the housing 100 or detachably connected.

[0375] The kit 20 also includes a third-item detection cell for performing a third-item test, which is integrally or detachably connected to the kit body 100.

[0376] The second and third test pools are located on the same side of the first test pool; or the second and third test pools are located on opposite sides of the first test pool.

[0377] The detection items in the first, second, and third detection pools can be selected from specific protein detection, biochemical detection, immunological detection, and routine blood tests. For example, routine blood tests can be performed using the front pool 120, mounting cavity 130, mounting base 160, and pressure chamber 140, while specific protein detection, biochemical detection, and immunological detection can be performed using the optical detection cup.

[0378] The detection items in the first, second, and third detection pools are the same, different, or not completely the same; or any one of the first, second, and third detection pools supports more than two detection items.

[0379] The housing 100 is provided with insertion holes, which may include rectangular insertion holes 107 and circular insertion holes 108. The second item testing pool is inserted through the insertion holes, or the second item testing pool is provided with flanges to be mounted and fitted with the insertion holes.

[0380] The second test pool includes a first pool body 207, a second pool body 208, and a connector 2071 that connects the first pool body 207 and the second pool body 208. The connector 2071 connects the first pool body 207 and the second pool body 208 to the housing 100 in the form of an assembly.

[0381] The first and / or second test pools are made of transparent plastic or glass. The openings of the first and / or second test pools are sealed with a membrane, which can be punctured by the puncture head 204 on the housing 100 before use.

[0382] The reagent kit 20 provided in this embodiment has a novel structure and multiple detachable options, which can adapt to a variety of different detection needs.

[0383] Please refer to the twenty-fifth embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a box body 100. The box body 100 includes a front chamber 120, a mounting cavity 130, and a pressure application cavity 140. The front chamber 120 and the mounting cavity 130 are connected, and the pressure application cavity 140 is connected to the mounting cavity 130. The pressure application cavity 140 is vertically arranged, and the mounting cavity 130 is horizontally arranged.

[0384] The opening end of the mounting cavity 130 faces the outer surface of the box 100. The reagent kit 20 also includes a mounting base 160, which is connected to the mounting cavity 130. The mounting base 160 is provided with an axial drainage cavity 167, which is connected to the pressure application cavity 140.

[0385] A drainage gap is formed between the mounting cavity 130 and the mounting base 160, and the axial drainage cavity 167 is connected to the pressure application cavity 140 through this drainage gap.

[0386] The mounting base 160 is provided with a radial liquid outlet groove 1611, and the axial drainage cavity 167 is connected to the pressure application cavity 140 through the radial liquid outlet groove 1611; the mounting base 160 is provided with a guide groove 1612 near the radial liquid outlet groove 1611, and the axial drainage cavity 167 is connected to the pressure application cavity 140 through the radial liquid outlet groove 1611 and the guide groove 1612.

[0387] The mounting base 160 is provided with a microporous plate 170 and a rear chamber electrode 165, and the front chamber 120 is provided with a front chamber electrode 121. The front chamber electrode 121 and the rear chamber electrode 165 are respectively located on both sides of the microporous plate 170 at intervals.

[0388] The mounting base 160 and the mounting cavity 130 are engaged by snap-fit, threaded fit, interference fit, laser welding fit, or adhesive fit.

[0389] The kit 20 also includes an inner sealing ring 164 and an outer sealing ring 166. The inner sealing ring 164 is disposed between the microporous sheet 170 and the box body 100, and the outer sealing ring 166 is disposed between the assembly base 160 and the box body 100.

[0390] In this embodiment, there are two sets of forepool 120 and mounting cavity 130, and one pressure-acting cavity 140 is connected to the two sets of mounting cavities 130.

[0391] The reagent kit 20 provided in this embodiment has a novel structure. Its front chamber 120, mounting cavity 130 and pressure application cavity 140 are interconnected and arranged vertically in sequence. The opening end of the pressure application cavity 140 is located on the upper surface of the box body 100. The negative pressure source can be applied from above the box body 100, which simplifies the structure of the box body 100 and avoids the box body 100 having too many protruding structures on the side.

[0392] Please refer to the twenty-sixth embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a plurality of first pool positions, the centers of which are arranged approximately in a first straight line or a first arc.

[0393] The kit 20 also includes a plurality of second pool positions arranged in a roughly second straight line, with the first and second straight lines arranged in parallel or perpendicular intervals.

[0394] Alternatively, the kit 20 includes a plurality of second pool positions arranged in a second arc at the center, with the first and second arcs arranged in parallel and spaced apart, and the arcs can be corresponding arcs with central angles of 0 to 360 degrees.

[0395] Multiple first cell positions and / or multiple second cell positions include impedance detection cells and / or optical detection cells.

[0396] The multiple first pool positions and / or multiple second pool positions also include a diluent pool 111, which is used to encapsulate the diluent; the multiple first pool positions and / or multiple second pool positions also include a hemolysin pool 106, which is used to encapsulate the hemolysin; or the multiple first pool positions and / or multiple second pool positions also include a sample dilution pool 112, which is used for sample dilution.

[0397] The impedance detection cell is equipped with a light transmission detection window for optical detection. Specifically, one of the front cells 120 is equipped with a light transmission detection window for optical detection. The front cell 120 can be made entirely of transparent plastic. The light transmittance and smoothness of the light transmission detection window can be the same as or higher than other parts of the front cell 120.

[0398] The multiple first pool positions and / or multiple second pool positions also include several insertion holes.

[0399] Multiple first pool positions and / or multiple second pool positions include at least one tip header containment pool (101, 102, 103) and / or sample containment pool 105.

[0400] The multiple first pool positions and / or multiple second pool positions include at least one puncture head receiving pool 104. The kit 20 provided in this application is a biodegradable plastic kit 20. By arranging the multiple pool positions in a straight line, it is convenient for the pipetting device to move along a shorter path during automated detection, wherein the pipetting device is used to transfer and mix the liquids in each pool.

[0401] Please refer to the twenty-seventh embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a box body 100. The box body 100 is provided with a detection area (such as the area formed by the pre-pool 120, the mounting cavity 130, the pressure application cavity 140, and the mounting base 160), a reagent area (such as the area formed by the diluent pool 111, the hemolysin pool 106, and the sample container pool 105), and an accessory placement area (such as the area formed by the tip head container pools 101, 102, 103 and the puncture head container pool 104).

[0402] The housing 100 also includes an expandable area (the area formed by the rectangular insertion hole 107 and the circular insertion hole 108 in the figure). The expandable area is located close to the detection area, on one side of the detection area, or on one side of the reagent area.

[0403] The attachment area is located near the reagent area, and the testing area is located near the reagent area.

[0404] The detection area includes an impedance detection cell and / or an optical detection cell, the optical detection cell including those for detecting any one of the parameters HGB, CRP, and SAA.

[0405] The reagent area includes at least one of a diluent pool, a hemolysin pool, and a sample container 105.

[0406] The reagent area includes a pool area for detachably housing at least one of a diluent container, a hemolysin container 206, and a sample holding pool 105.

[0407] The accessory placement area includes placement positions for tip heads 201, 202, 203 and / or puncture head 204.

[0408] The expandable area includes a detachable optical detection cell and / or impedance detection cell, the optical detection cell including those for detecting any one of the HGB, CRP, and SAA parameters.

[0409] The reagent kit 20 provided in this application is a biodegradable plastic reagent kit 20. Through the partitioning setting, the pipetting device can be moved along a shorter path during automatic detection.

[0410] Please refer to the twenty-eighth embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a box body 100. The box body 100 is provided with a detection area (such as the area formed by the pre-pool 120, the mounting cavity 130, the pressure application cavity 140, and the mounting base 160), a reagent area (such as the area formed by the diluent pool 111, the hemolysin pool 106, and the sample container pool 105), and an accessory placement area (such as the area formed by the tip head container pools 101, 102, 103 and the puncture head container pool 104). The reagent area is located close to the accessory placement area.

[0411] The housing 100 also includes an expandable area (the area formed by the rectangular insertion hole 107 and the circular insertion hole 108 in the figure), which is located close to the detection area. Alternatively, the expandable area may be located close to the side of the detection area or close to the side of the reagent area.

[0412] The reagent area includes multiple reagent pools, which are arranged in a roughly straight line or an arc.

[0413] The detection area includes an impedance detection cell and / or an optical detection cell, the optical detection cell including those for detecting any one of the parameters HGB, CRP, and SAA.

[0414] The accessory placement area includes placement positions for tip heads 201, 202, 203 and / or puncture head 204.

[0415] The expandable area includes a detachable optical detection cell and / or impedance detection cell, the optical detection cell including those for detecting any one of the HGB, CRP, and SAA parameters.

[0416] The reagent area includes at least two pool areas, each including a bottomed pool and / or a bottomless insertion port.

[0417] The attachment area includes one or more bottomed pools for mounting tip heads 201, 202, 203 and / or puncture heads 204.

[0418] The expandable area is provided with a circular insertion hole 108 and a rectangular insertion hole 107, which are used to install detection cells, which are optical detection cells and / or impedance detection cells. The reagent kit 20 provided in this application is a biodegradable plastic reagent kit 20, and the partitioned setting facilitates the movement of the pipetting device with a shorter path during automatic detection.

[0419] Please refer to the twenty-ninth embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a housing 100. The reagent kit 20 is used for sample testing. The housing 100 is provided with a support portion so that the reagent kit 20 can be stably supported on the support surface.

[0420] In one embodiment, the box body 100 includes two support plates (not shown), which serve as support components. The support plates may be marked with barcodes, QR codes, or identification chips to record relevant parameters of the reagent kit 20.

[0421] The box body 100 has at least one support platform or support surface near the center of gravity of the reagent kit 20, and the support platform or support surface serves as a support.

[0422] In one embodiment, the box 100 has three support protrusions that form a triangle, and the projection of the center of gravity of the reagent kit 20 falls within the area enclosed by the triangle.

[0423] In one embodiment, the box 100 includes at least one pool, the bottom of which can serve as a support.

[0424] Optionally, the support plate, support platform, support surface, or support protrusion, together with the bottom of the pool body, serve as the support structure.

[0425] The pool body includes a receiving cavity and a skirt 124 extending downward from the receiving cavity (reference). Figure 18 The structure below the bottom 123 of the forecourt 120 has a skirt 124 as a support and a cavity that can hold reagents, samples, or accessories (201-204).

[0426] The box 100 provided in this application is a biodegradable plastic box. The reagent kit 20 is provided with an impedance detection cell and / or an optical detection cell.

[0427] The reagent kit 20 provided in this embodiment has multiple pools, and may form stable support through the bottom of the pools and / or additional support parts, so as to prevent the reagent kit 20 from easily tipping over.

[0428] Please refer to the thirtieth embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a box body 100. The box body 100 includes multiple pools, each pool including a receiving cavity and a support connected to the receiving cavity. The support can be a solid structure or a hollow structure. Setting a solid or hollow support can increase the relative height of the bottom of the receiving cavity, which can facilitate reagent aspiration. The corresponding pipette does not need to be inserted too deeply, thereby saving the pre-stored amount of reagent. If it is a solid structure, the center of gravity of the box body 100 can also be lowered, making the whole more stable; if it is hollow, it can save the plastic material of the box body 100.

[0429] The height of the support is greater than or equal to the wall thickness of the receiving cavity. The bottom of the receiving cavity can be conical, specifically a conical arc bottom, a triangular pyramid or a multi-faceted pyramid bottom, with the conical bottom extending into the hollow area at the bottom of the support; or the bottom of the receiving cavity can be flat, with the receiving cavity connected to the support through the flat bottom.

[0430] When the support is a hollow structure, the bottom of the support has an open end or a closed end.

[0431] The cavity and support are integrally formed or detachably connected.

[0432] When the support is a hollow structure, the cross-section of the hollow structure is square, circular, polygonal or irregular, and the height of the support is 0.1 to 0.8 times the height of the pool.

[0433] The aforementioned pool can be any pool such as a reagent pool, a detection pool, a sample dilution pool 112, or an accessory placement pool (101-104).

[0434] The aforementioned detection cell is used for impedance detection or optical detection.

[0435] This application embodiment also provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 that cooperates with the reagent kit 20. The detection seat 300 is used for sample analysis and detection. The detection seat 300 is provided with a power supply component that is electrically connected to the electrode 121 of the front cell 120 and the electrode 165 of the rear cell. The detection seat 300 may also be provided with optical detection components located on opposite sides of the optical detection cell (e.g., the first cell 207).

[0436] The reagent kit 20 provided in this embodiment can facilitate the pipetting of liquids by setting the bottom of the tank at different heights. The pipetting device does not need to be inserted too deeply, thereby saving the amount of pre-stored reagents.

[0437] Please refer to the thirty-first embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a box 100. The box 100 is provided with an accessory placement area (101-104) for placing accessories (201-204).

[0438] The accessory placement area has a leak-proof structure on its lower side, which can be a bottomed pool. The leak-proof structure is integrally formed with the box body 100 or can be detachably connected. The accessory placement area has placement holes, and accessories (201-204) are placed in the placement holes.

[0439] The diameter of the placement hole is less than or equal to the maximum radial dimension of the accessory (201-204) to prevent the accessory (201-204) from being completely sunk into the placement hole and to prevent the bottom of the accessory (201-204) from contacting and deforming with the bottom of the pool when the accessory (201-204) is inserted.

[0440] The box body 100 has a receiving cavity corresponding to the placement hole, and the accessories (201-204) are housed in the receiving cavity. The bottom of the receiving cavity is a sealed structure.

[0441] The accommodating cavity and the box body 100 are integrally formed or detachably connected.

[0442] The depth of the inner cavity of the receiving cavity is greater than or equal to the length of the attachment (201-204) extending into the receiving cavity, so as to avoid the attachment (201-204) protruding too much from the surface of the box 100 when it is placed in the receiving cavity, and at the same time, it can prevent the bottom of the attachment (201-204) from contacting and deforming with the bottom of the pool when the attachment (201-204) is inserted.

[0443] The length of the attachment (201-204) extending into the accommodating cavity is 0.4 to 1 times the length of the attachment.

[0444] This application embodiment also provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 that cooperates with the reagent kit 20. The detection seat 300 is used for sample analysis and detection.

[0445] The kit 20 provided in this embodiment prevents leakage of residual liquid carried on the surface of the attachments by receiving the attachments in a bottom pool during attachment rejection.

[0446] Please refer to the thirty-second embodiment as well. Figure 15 and Figure 20 This application provides a detection cup assembly, which includes a first pool 207 and a second pool 208 connected to the first pool 207.

[0447] The first pool body 207 and the second pool body 208 are connected by a connector 2071, which is integrally formed with or detachably connected to at least one of the first pool body 207 and the second pool body 208.

[0448] The connector 2071 is provided with at least one cup holder 2072, which is detachably connected to the first pool body 207 or the second pool body 208.

[0449] In one embodiment, the connector 2071 is provided with a cup holder 2072 and the connector is integrally connected to the first pool body 207. The cup holder 2072 is provided with a placement hole, the shape of which may be the same as or different from the cross-sectional shape of the first pool body 207.

[0450] In one embodiment, the connector 2071 is provided with two cup holders 2072, and the two cup holders 2072 are respectively provided with a first placement hole and a second placement hole, the first placement hole and the second placement hole may have the same shape or different shape.

[0451] The first chamber 207 is a test cup, and the second chamber 208 is a reagent cup. During testing, the reagent in the second chamber 208 is added to the first chamber 207 to prepare the sample to be tested before testing.

[0452] The first pool body 207 is provided with an optical detection window for optical detection, such as optical detection by transmitted light or optical detection by scattered light.

[0453] The cross-sectional shapes of the first pool 207 and the second pool 208 may be the same or different; or the heights of the first pool 207 and the second pool 208 may be the same or different. This differentiated design can prevent mistakes and facilitate assembly and identification.

[0454] This application embodiment also provides a reagent kit 20, which includes a box body 100 and the aforementioned test cup assembly. The box body 100 is provided with an installation part, and the test cup assembly is provided with a mating part. The installation part is connected to the mating part.

[0455] The mating part can be a protrusion or a recess provided on the side surface of the test cup assembly, which is connected to the mounting part; or the mating part can be a positioning flange provided on the test cup assembly, which is connected to the mounting part, for example, by using a flange structure to hang it on the insertion hole on the box 100.

[0456] The detection cup assembly provided in this embodiment has a novel structure. Specific detection items can be equipped with corresponding required reagents on the cup holder 2072, which can greatly improve detection efficiency and allow for flexible selection of detection items.

[0457] Please refer to the thirty-third embodiment as well. Figures 15 to 20 This application provides a reagent kit 20, which includes a housing 100 and a mounting base 160.

[0458] The housing 100 includes a front chamber 120, on which a front chamber 120 electrode 121 is mounted; a mounting base 160 is connected to the housing 100, on which a rear chamber electrode 165 is mounted, and the front chamber 120 electrode 121 and the rear chamber electrode 165 are spaced apart.

[0459] In this embodiment, the axis of the front chamber electrode 120 and the axis of the rear chamber electrode 165 are approximately the same straight line. Experimental verification shows that the detection accuracy is relatively high when the axis of the front chamber electrode 120 and the rear chamber electrode 165 are coaxial. Both the front chamber electrode 120 and the rear chamber electrode 165 are columnar electrodes, and the manufacturing and assembly processes are relatively simple.

[0460] The mounting base 160 is provided with an axial drainage cavity 167, which is connected to the front pool 120 through a microporous plate 170. The front pool 120 electrode 121 and the rear pool electrode 165 are located on both sides of the microporous plate 170.

[0461] The microporous sheet 170 includes a sheet body 171, which has micropores 172 that allow cells to pass through one by one. The microporous sheet 170 is mounted on the mounting base 160 or the box body 100, or the microporous sheet 170 and the mounting base 160 or the box body 100 are integrally formed.

[0462] The axes of the forecell electrode 121, the rear cell electrode 165, and the microporous plate 170 are approximately aligned.

[0463] The front chamber 120 electrode 121 protrudes, is flush with, or is recessed in the inner wall of the front chamber 120; the rear chamber electrode 165 protrudes, is flush with, or is recessed in the bottom wall of the axial drainage cavity 167. The two ends of the front chamber 120 electrode 121 and the rear chamber electrode 165 are not particularly limited. During detection, the front chamber 120 electrode 121 and the rear chamber electrode 165 are in contact with the test liquid. The outer ends of the front chamber 120 electrode 121 and the rear chamber electrode 165 are used to connect to an external power supply assembly.

[0464] The housing 100 is provided with a mounting cavity 130, and the mounting base 160 includes a mounting cylinder 168. The mounting cavity 130 and the mounting cylinder 168 are coaxially connected.

[0465] The assembly cylinder 168 and the mounting cavity 130 are fitted by snap-fit, threaded fit, interference fit, laser welding fit, or adhesive fit.

[0466] The front cell electrode 121 is disposed on the housing 100 and protrudes, is flush with or is recessed on the outer side of the housing 100, and the rear cell electrode 165 is disposed on the mounting base 160 and protrudes, is flush with or is recessed on the outer side of the mounting base 160.

[0467] This application embodiment also provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 that cooperates with the reagent kit 20. The detection seat 300 is used for sample analysis and detection.

[0468] Please refer to the thirty-fourth embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a box body 100 and a grip portion 150, the grip portion 150 being disposed on the box body 100.

[0469] The grip portion 150 may be positioned near the center of the side of the box body 100 or near the center of the upper surface of the box body 100.

[0470] There are two grips 150, which are respectively located on opposite sides of the box body 100.

[0471] The grip portion 150 protrudes from the upper surface or side of the box body 100. The outer surface of the grip portion 150 is provided with a snap protrusion 151 or a snap recess.

[0472] The grip portion 150 has an anti-slip portion 152 at its top or outer side.

[0473] The grip portion 150 is an elastic element, and the side of the box body 100 is provided with a recessed portion 154, which is connected to the grip portion 150.

[0474] The grip portion 150 and the recessed portion 154 are either integrally connected or detachably connected.

[0475] The grip portion 150 is slidably connected to the recessed portion 154 so that the grip portion 150 and the box body 100 can move relative to each other. The grip portion 150 can extend and retract relative to the box body 100, making it convenient to store without taking up space. It can be pulled out when needed.

[0476] This application embodiment also provides a sample testing device, which includes the aforementioned reagent kit 20 and a testing seat 300 that cooperates with the reagent kit 20, with the gripping part 150 cooperating and connected with the testing seat 300.

[0477] Please refer to the thirty-fifth embodiment as well. Figure 15 and Figure 20 This application provides a reagent kit 20, which includes a housing 100 and a barrier portion 153.

[0478] The housing 100 includes at least one compartment (e.g., sample holding compartment 105); the compartment is used to hold test tubes (e.g., sample tube 205).

[0479] The blocking part 153 is provided on one side of the pool position and is used to hook the test tube cap (not shown in the figure) to prevent the opened test tube cap from being reset and covering the open end of the test tube.

[0480] In a specific embodiment, the blocking part 153 may protrude, be flush with or recessed on the upper surface or side surface of the box body 100.

[0481] In one embodiment, the blocking portion 153 includes a connector connected to the housing 100 and a stop portion extending from the connector. The end of the stop portion is provided with a hook portion extending toward the housing 100.

[0482] In another embodiment, the side surface of the box body 100 protrudes outward to form a blocking part 153; or the blocking part 153 is a groove provided on the upper surface of the box body 100; or the upper surface of the box body 100 is provided with a recessed part, the inner wall of the recessed part forms the blocking part 153, and the test tube cap can be inserted into the recessed part after the test tube is opened, and the size of the recessed part can correspond to the size of the test tube cap to achieve a relatively tight fit.

[0483] The barrier portion 153 is integrally formed with the box body 100 or is detachably connected. Specifically, the barrier portion 153 is connected to the box body 100 by snap-fit ​​connection, insertion connection, threaded connection, or detachable connection via screws / pins.

[0484] In other embodiments, the blocking part 153 is also slidably connected to the housing 100.

[0485] In this embodiment of the application, the pool position (e.g., sample holding pool 105) is located near the edge of the box body 100, and the blocking part 153 is located at the edge of the box body 100.

[0486] The above are merely embodiments of this application and do not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A POCT blood cell analyzer, characterized in that, The POCT blood cell analyzer includes: frame; The detection seat is slidably or rotatably disposed relative to the frame. The detection seat reciprocates between the loading station and the sample detection station. When the detection seat slides out or rotates out of the frame, it is located at the loading station to receive the reagent kit with an impedance detection cell or to allow the reagent kit to be removed. The impedance detection cell includes a front cell and a rear cell connected by micropores. The detection seat is provided with a power supply component that cooperates with impedance detection. The detection seat includes a main body and a metal shielding seat covering the outer periphery of the main body. A metal shielding cover that matches the metal shielding base is correspondingly disposed at the sample detection station, and the metal shielding cover is capable of lifting or rotating. The POCT blood cell analyzer also includes a pressure building system, which is connected to the gas path of the front chamber and / or the rear chamber. The pressure building system is used to provide pressure so that the test solution in the front chamber flows through the micropore to the rear chamber. It is also used to provide pressure to the front chamber or the rear chamber before the test solution is injected into the front chamber so that the micropore is unobstructed. A pipette connected to the gas path of the pressure-building system, the pipette being positioned above the detection seat, is used to perform corresponding operations on the reagent kit; The pressure-building system includes a pressure chamber, a pneumatic connection device, a first syringe, a second syringe, and a driving component. The first syringe is at least connected to the pressure chamber to establish positive or negative pressure in the pressure chamber. The pressure chamber is connected to the pneumatic connection device. The second syringe is connected to the pipette to aspirate and / or dispense samples and / or reagents. The driving component is used to simultaneously drive the first syringe and the second syringe. The first syringe and the second syringe are linked syringes. One end of the pressure-building system is provided with a pneumatic connection device that extends into the metal shielding cap and moves synchronously with the metal shielding cap. The reagent kit is provided with a pressure chamber that docks with the pneumatic connection device. The POCT blood cell analyzer also includes a first solenoid valve, a second solenoid valve, and a third solenoid valve. The first solenoid valve is used to selectively connect the first syringe to the pressure chamber and the second syringe. The second solenoid valve is used to selectively connect the first solenoid valve to the pressure chamber and the outside atmosphere. The third solenoid valve is used to selectively connect the pneumatic connection device to the pressure chamber.

2. The POCT blood cell analyzer according to claim 1, characterized in that: The POCT blood cell analyzer also includes a solution preparation station; The solution preparation worker is located between the loading station and the sample testing station; or The solution preparation station and the sample testing station are the same station.

3. The POCT blood cell analyzer according to claim 1, characterized in that: The detection seat is a semi-enclosed shape with an opening at the top, and the detection seat is snapped into place with the reagent kit.

4. The POCT blood cell analyzer according to claim 1, characterized in that: The metal shielding cover is located above the detection base and is configured to move in the vertical direction.

5. A method of using a POCT blood cell analyzer, characterized in that, Based on the POCT hematology analyzer according to any one of claims 1-4, the method of use includes: The test stand slides out or rotates out of the frame to receive the reagent kit at the loading station; The detection seat slides into or screws into the frame and moves to the liquid preparation station; The pipette moves to load the mounting head pre-placed on the reagent kit to perform the corresponding operation.

6. The method of use according to claim 5, characterized in that, Also includes: The testing station moves from the liquid preparation station to the sample testing station below the metal shielding cover; The metal shielding cover moves downwards to engage with the detection seat.

7. The method of use according to claim 5, characterized in that: Also includes: The pressure-building system connected to the metal shielding cover begins to provide pressure, draining the liquid from the impedance detection cell on the kit.

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