Glass cover plate thickness adjustable high-power microscope objective and image taking device

Abstract

The application discloses a glass cover plate thickness-adjustable high-power microscopic objective and an image taking device, and relates to the technical field of optical instruments.The glass cover plate thickness-adjustable high-power microscopic objective comprises a first lens group with positive refractive power, a second lens group with positive refractive power, a third lens group with negative refractive power and a fourth lens group with positive refractive power which are sequentially arranged from an object plane to an image plane.The glass cover plate thickness-adjustable high-power microscopic objective satisfies the following conditions: 0.5<|f2 / f1|<6.0; 0.6<|f3 / f1|<7.0; 0.1<|f3 / f2|<3.8; 0.5<|f4 / f1|<8.0; 0.1<|f4 / f2|<3.5; and 0.1<|f4 / f3|<3.2.The application has the characteristics of a large adjustable range of the glass cover plate, a large numerical aperture, a long working distance, high resolution performance and small chromatic aberration.

Description

Technical Field

[0001] This invention relates to the field of optical technology, and more specifically to a high-magnification microscope objective with an adjustable glass cover plate thickness and an apparatus using the objective, such as a microscope equipped with the objective optical system and an image acquisition device equipped with the objective optical system. Background Technology

[0002] With the rapid development of the biomedical and electronics industries, there is a growing demand for microscopic observation of biological samples or electronic components under glass cover plates of varying thicknesses. Typically, a single objective lens can only guarantee image quality when using a glass cover plate of a specific thickness. To avoid frequent objective lens changes due to the need to adapt to various glass cover plate thicknesses, an effective approach is to make the objective lens spacing variable to prevent potential imaging defects. Objective lens design optimization must simultaneously consider various glass cover plate thicknesses and their application scenarios. Correcting on-axis aberrations, chromatic aberration, and field curvature is very challenging, especially when longer working distances and thicker glass cover plates are required.

[0003] Japanese Patent Document JP 5277324 discloses a 50X microscope objective (i.e., a high-power microscope) with an adjustable glass cover from 0mm to 1.2mm in its four, five and six specific embodiments. The maximum working distance of this high-power microscope is 3.49mm, and its glass cover adjustment range and working distance are relatively small, and its numerical aperture is not large enough. Summary of the Invention

[0004] In view of the above-mentioned defects of the prior art, the present invention provides a high-magnification microscope objective with adjustable glass cover thickness and an image acquisition device to solve the problems of insufficient adjustable range of glass cover, small numerical aperture, short working distance, large chromatic aberration and low resolution.

[0005] The present invention adopts the following technical solution:

[0006] On one hand, a high-magnification microscope objective with adjustable cover plate thickness includes: a first lens group with positive refractive power, a second lens group with positive refractive power, a third lens group with negative refractive power, and a fourth lens group with positive refractive power, arranged sequentially from the object plane to the image plane. The combination of these multiple lens groups enables the optical system of the high-magnification microscope objective with adjustable cover plate thickness to possess strong positive refractive power, thereby ensuring that the objective system has a larger numerical aperture and higher optical performance.

[0007] To enable high-magnification microscope objectives to have a larger numerical aperture, higher resolution, and a longer working distance, the high-magnification microscope objectives with adjustable cover plate thickness must meet the following conditions:

[0008] 0.5 < |f2 / f1| < 6.0;

[0009] 0.6 < |f3 / f1| < 7.0;

[0010] 0.1 < |f3 / f2| < 3.8;

[0011] 0.5 < |f4 / f1| < 8.0;

[0012] 0.1 < |f4 / f2| < 3.5;

[0013] 0.1 < |f4 / f3| < 3.2;

[0014] Where f1 is the focal distance of the first lens group, f2 is the focal distance of the second lens group, f3 is the focal distance of the third lens group, and f4 is the focal distance of the fourth lens group.

[0015] Preferably, the first lens group is closest to the object and includes one or more cemented lenses. Specifically, the first lens, the second lens, and the third lens are arranged sequentially along the direction from the object plane to the image plane. The first lens has positive refractive power, the second lens has positive refractive power, and the third lens has negative refractive power.

[0016] Preferably, the second lens group includes one or more cemented lenses, specifically a fourth lens and a fifth lens are arranged sequentially along the object plane to the image plane; the second lens group is a focusing group, which adapts to the change in the thickness of the glass cover by changing the air gap between the second lens group and the first lens group and the third lens group; the fourth lens has negative refractive power and the fifth lens has positive refractive power.

[0017] Preferably, the third lens group includes two or more cemented lenses, specifically a sixth lens, a seventh lens, an eighth lens, and a ninth lens arranged sequentially along the object plane to the image plane; the sixth lens has positive refractive power, the seventh lens has negative refractive power, the eighth lens has positive refractive power, and the ninth lens has negative refractive power.

[0018] Preferably, the fourth lens group includes one or more cemented lenses, specifically a tenth lens, an eleventh lens, and a twelfth lens arranged sequentially along the object plane to the image plane; the tenth lens has positive refractive power, the eleventh lens has negative refractive power, and the twelfth lens has negative refractive power.

[0019] Preferably, in order to better control distortion and field curvature, the first lens group, the second lens group, the third lens group, and the fourth lens group must satisfy the following condition:

[0020] 0.1 <f11 / f1<3.5;

[0021] 0.1 <f12 / f1<3.2;

[0022] -6.0 <f13 / f1<-0.2;

[0023] -2.0 <f21 / f2<-0.2;

[0024] 0.1 <f22 / f2<1.2;

[0025] -1.2 <f31 / f3<-0.1;

[0026] 0.05 <f32 / f3<1.5;

[0027] -2.0 <f33 / f3<0;

[0028] 0 <f34 / f3<1.0;

[0029] 0 <f41 / f4<1.2;

[0030] -2.5 <f42 / f4<-0.1;

[0031] -2.5 <f43 / f4<0;

[0032] Wherein, f11 is the focal distance of the first lens, f12 is the focal distance of the second lens, f13 is the focal distance of the third lens, f21 is the focal distance of the fourth lens, f22 is the focal distance of the fifth lens, f31 is the focal distance of the sixth lens, f32 is the focal distance of the seventh lens, f33 is the focal distance of the eighth lens, f34 is the focal distance of the ninth lens, f41 is the focal distance of the tenth lens, f42 is the focal distance of the eleventh lens, and f43 is the focal distance of the twelfth lens.

[0033] Preferably, in order to correct the coma and on-axis aberrations of the optical system of a high-magnification microscope objective with adjustable cover plate thickness, and to further correct the field curvature and distortion of the microscope objective optical system, the refractive indices of the first lens, second lens, third lens, fourth lens, fifth lens, sixth lens, seventh lens, eighth lens, ninth lens, tenth lens, eleventh lens, and twelfth lens must meet the following conditions:

[0034] N11≤2.0;

[0035] N12≥1.4;

[0036] N13≥1.4;

[0037] N21≥1.5;

[0038] N22≥1.3;

[0039] N31≥1.3;

[0040] N32≥1.5;

[0041] N33≤1.7;

[0042] N34≤1.7;

[0043] N41≥1.6;

[0044] N42≥1.6;

[0045] N43≥1.3;

[0046] Wherein, N11 is the refractive index of the first lens, N12 is the refractive index of the second lens, N13 is the refractive index of the third lens, N21 is the refractive index of the fourth lens, N22 is the refractive index of the fifth lens, N31 is the refractive index of the sixth lens, N32 is the refractive index of the seventh lens, N33 is the refractive index of the eighth lens, N34 is the refractive index of the ninth lens, N41 is the refractive index of the tenth lens, N42 is the refractive index of the eleventh lens, and N43 is the refractive index of the twelfth lens.

[0047] Preferably, in order to reduce chromatic aberration in the objective lens optical system, the Abbe numbers of the first, second, third, fourth, fifth, sixth, seventh, eighth, ninth, tenth, eleventh, and twelfth lenses must meet the following conditions:

[0048] V11≥40;

[0049] V12≤90;

[0050] V13≥35;

[0051] V21≤65;

[0052] V22≤90;

[0053] V31≥90;

[0054] V32≤40;

[0055] V33≥75;

[0056] V34≥40;

[0057] V41≤40;

[0058] V42≤65;

[0059] V43≤70;

[0060] Wherein, V11 is the Abbe number of the first lens, V12 is the Abbe number of the second lens, V13 is the Abbe number of the third lens, V21 is the Abbe number of the fourth lens, V22 is the Abbe number of the fifth lens, V31 is the Abbe number of the sixth lens, V32 is the Abbe number of the seventh lens, V33 is the Abbe number of the eighth lens, V34 is the Abbe number of the ninth lens, V41 is the Abbe number of the tenth lens, V42 is the Abbe number of the eleventh lens, and V43 is the Abbe number of the twelfth lens.

[0061] Preferably, in order to further reduce on-axis aberrations and coma, the thicknesses of the first lens, second lens, third lens, fourth lens, fifth lens, sixth lens, seventh lens, eighth lens, ninth lens, tenth lens, eleventh lens, and twelfth lens satisfy the following conditions:

[0062] 0.1 <T11 / T12<0.8;

[0063] 0.5 <T12 / T13<1.5;

[0064] 0.05 <T21 / T22<0.6;

[0065] 1.0 <T31 / T32<5.0;

[0066] 0.05 <T32 / T33<0.5;

[0067] 0.5 <T33 / T34<5.0;

[0068] 1.5 <T41 / T42<7.5;

[0069] 0.4 <T42 / T43<4.0;

[0070] Wherein, T11 is the thickness of the first lens on the optical axis, T12 is the thickness of the second lens on the optical axis, T13 is the thickness of the third lens on the optical axis, T21 is the thickness of the fourth lens on the optical axis, T22 is the thickness of the fifth lens on the optical axis, T31 is the thickness of the sixth lens on the optical axis, T32 is the thickness of the seventh lens on the optical axis, T33 is the thickness of the eighth lens on the optical axis, T34 is the thickness of the ninth lens on the optical axis, T41 is the thickness of the tenth lens on the optical axis, T42 is the thickness of the eleventh lens on the optical axis, and T43 is the thickness of the twelfth lens on the optical axis.

[0071] On the other hand, an image capturing device includes a high-magnification microscope objective with an adjustable glass cover plate thickness.

[0072] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0073] (1) The present invention sets the first lens group, the second lens group, the third lens group and the fourth lens group in the high magnification microscope objective with adjustable glass cover plate thickness so that the first lens group has positive refractive power, the second lens group has positive refractive power, the third lens group has negative refractive power and the fourth lens group has positive refractive power, so that the objective lens optical system has good optical performance.

[0074] (2) By limiting the focal distance, refractive index, Abbe number and thickness of the first lens group, the second lens group, the third lens group and the fourth lens group, the present invention further corrects the field curvature, distortion and aberration sensitivity of the high magnification microscope objective optical system with adjustable glass cover thickness, thereby ensuring the optical performance of the high magnification microscope objective optical system with adjustable glass cover thickness. This objective optical system has the advantages of a large adjustable range of glass cover, large numerical aperture, long working distance, high resolution and chromatic aberration.

[0075] The above and other objects, advantages and features of the present invention will become more apparent to those skilled in the art from the following detailed description of specific embodiments of the invention in conjunction with the accompanying drawings. Attached Figure Description

[0076] Figure 1 This is a lens configuration diagram of the high-magnification microscope objective optical system with adjustable glass cover thickness according to Embodiment 1 of the present invention.

[0077] Figure 2 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention when the glass cover thickness D1 is 0 mm.

[0078] Figure 3 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention when the glass cover thickness D1 is 0 mm.

[0079] Figure 4 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention when the glass cover thickness D1 is 0 mm.

[0080] Figure 5 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention when the glass cover thickness D1 is 0.5 mm.

[0081] Figure 6 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention, when the glass cover thickness D1 is 0.5 mm.

[0082] Figure 7This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention when the glass cover thickness D1 is 0.5 mm.

[0083] Figure 8 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention, when the glass cover thickness D1 is 1.0 mm.

[0084] Figure 9 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention, when the glass cover thickness D1 is 1.0 mm.

[0085] Figure 10 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention when the glass cover thickness D1 is 1.0 mm.

[0086] Figure 11 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention, when the glass cover thickness D1 is 1.5 mm.

[0087] Figure 12 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention, when the glass cover thickness D1 is 1.5 mm.

[0088] Figure 13 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention when the glass cover thickness D1 is 1.5 mm.

[0089] Figure 14 This is a lens configuration diagram of the high-magnification microscope objective optical system with adjustable glass cover thickness according to Embodiment 2 of the present invention.

[0090] Figure 15 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention when the glass cover thickness D1 is 0 mm.

[0091] Figure 16 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention when the glass cover thickness D1 is 0 mm.

[0092] Figure 17 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention when the thickness D1 of the glass cover is 0 mm.

[0093] Figure 18 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention, when the glass cover thickness D1 is 0.5 mm.

[0094] Figure 19 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention, when the glass cover thickness D1 is 0.5 mm.

[0095] Figure 20 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention when the thickness D1 of the glass cover is 0.5 mm.

[0096] Figure 21 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention, when the glass cover thickness D1 is 1.0 mm.

[0097] Figure 22 This is a field curve diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention, when the glass cover thickness D1 is 1.0 mm.

[0098] Figure 23 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention when the thickness D1 of the glass cover is 1.0 mm.

[0099] Figure 24 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention, when the glass cover thickness D1 is 1.5 mm.

[0100] Figure 25 This is a field curve diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention, when the glass cover thickness D1 is 1.5 mm.

[0101] Figure 26 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention when the glass cover thickness D1 is 1.5 mm.

[0102] Figure 27 This is a lens configuration diagram of the high-magnification microscope objective optical system with adjustable glass cover thickness according to Embodiment 3 of the present invention.

[0103] Figure 28 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention when the glass cover thickness D1 is 0 mm.

[0104] Figure 29 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention when the glass cover thickness D1 is 0 mm.

[0105] Figure 30This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention when the thickness D1 of the glass cover is 0 mm.

[0106] Figure 31 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention when the glass cover thickness D1 is 0.5 mm.

[0107] Figure 32 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention, when the glass cover thickness D1 is 0.5 mm.

[0108] Figure 33 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention when the thickness D1 of the glass cover is 0.5 mm.

[0109] Figure 34 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention, when the glass cover thickness D1 is 1.0 mm.

[0110] Figure 35 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention, when the glass cover thickness D1 is 1.0 mm.

[0111] Figure 36 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention when the thickness D1 of the glass cover is 1.0 mm.

[0112] Figure 37 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention, when the glass cover thickness D1 is 1.5 mm.

[0113] Figure 38 This is a field curvature diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention, when the glass cover thickness D1 is 1.5 mm.

[0114] Figure 39 This is a distortion diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness (D1) of 1.5 mm according to Embodiment 3 of the present invention. Detailed Implementation

[0115] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0116] The optical system of a high-magnification microscope objective with adjustable glass cover thickness, and the image acquisition device equipped with the high-magnification microscope objective with adjustable glass cover thickness, which are disclosed in this invention, will be described below with reference to the accompanying drawings.

[0117] Example 1

[0118] like Figure 1 As shown, the high-magnification microscope objective OB with adjustable glass cover thickness in the first embodiment of the present invention includes an object plane 700, a glass cover plate 701, a first lens group 71, a second lens group 72, a third lens group 73, and a fourth lens group 74.

[0119] The first lens group 71 includes a first lens 711 with positive refractive power, a second lens 712 with positive refractive power, and a third lens 713 with negative refractive power. The object-facing surface of the first lens 711 is designated as the first surface, and the image-facing surface is designated as the second surface. The object-facing surface of the second lens 712 is designated as the first surface, and the image-facing surface is designated as the second surface. The object-facing surface of the third lens 713 is designated as the first surface, and the image-facing surface is designated as the second surface.

[0120] The second lens group 72 includes a fourth lens 721 with negative refractive power and a fifth lens 722 with positive refractive power. The object-facing surface of the fourth lens 721 is the first surface, and the image-facing surface is the second surface. The object-facing surface of the fifth lens 722 is the first surface, and the image-facing surface is the second surface.

[0121] The third lens group 73 includes a sixth lens 731 with positive refractive power, a seventh lens 732 with negative refractive power, an eighth lens 733 with positive refractive power, and a ninth lens 734 with negative refractive power. The object-facing surface of the sixth lens 731 is a first surface, and the image-facing surface is a second surface. The object-facing surface of the seventh lens 732 is a first surface, and the image-facing surface is a second surface. The object-facing surface of the eighth lens 733 is a first surface, and the image-facing surface is a second surface. The object-facing surface of the ninth lens 734 is a first surface, and the image-facing surface is a second surface.

[0122] The fourth lens group 74 includes a tenth lens 741 with positive refractive power, an eleventh lens 742 with negative refractive power, and a twelfth lens 743 with negative refractive power. The object-facing surface of the tenth lens 741 is the first surface, and the image-facing surface is the second surface. The object-facing surface of the eleventh lens 742 is the first surface, and the image-facing surface is the second surface. The object-facing surface of the twelfth lens 743 is the first surface, and the image-facing surface is the second surface.

[0123] In this embodiment of the microscope objective optical system with adjustable glass cover thickness, the field of view is 25 mm and the numerical aperture (NA) is 0.57.

[0124] The first lens 711 has a focal length f11 of 12.41, a refractive index N11 of 1.90, an Abbe number V11 of 46.4, and a thickness T11 of 2.00.

[0125] The second lens 712 has a focal length f12 of 10.55, a refractive index N12 of 1.50, an Abbe number V12 of 81.6, and a thickness T12 of 4.2.

[0126] The third lens 713 has a focal length f13 of -31.34, a refractive index N13 of 1.61, an Abbe number V13 of 42.1, and a thickness T21 of 3.8.

[0127] The fourth lens 721 has a focal length f21 of -20.78, a refractive index N21 of 1.71, an Abbe number V21 of 59.0, and a thickness T22 of 1.1.

[0128] The fifth lens 722 has a focal length f22 of 13.63, a refractive index N22 of 1.50, an Abbe number V22 of 81.6, and a thickness T31 of 4.4.

[0129] The sixth lens 731 has a focal length f31 of 16.48, a refractive index N31 of 1.44, an Abbe number V31 of 94.9, and a thickness T32 of 3.8.

[0130] The seventh lens 732 has a focal length f32 of -14.81, a refractive index N32 of 1.72, an Abbe number V32 of 33.8, and a thickness T33 of 1.1.

[0131] The eighth lens 733 has a focal length f33 of 13.09, a refractive index N33 of 1.50, an Abbe number V33 of 81.6, and a thickness T34 of 4.8.

[0132] The ninth lens 734 has a focal length f34 of -7.59, a refractive index N34 of 1.61, an Abbe number V34 of 45.4, and a thickness T35 of 2.4.

[0133] The tenth lens 741 has a focal length f41 of 7.34, a refractive index N41 of 1.80, an Abbe number V41 of 35.7, and a thickness T36 of 3.8.

[0134] The eleventh lens 742 has a focal length f42 of -14.35, a refractive index N42 of 1.70, an Abbe number V42 of 56.2, and a thickness T36 of 1.0.

[0135] The twelfth lens 743 has a focal length f43 of -8.76, a refractive index N43 of 1.52, an Abbe number V43 of 64.2, and a thickness T36 of 1.0.

[0136] Other optical parameters of the objective lens optical system are shown in Tables 1-1 and 1-2. In Table 1-2, D1 represents the thickness of the microscope objective lens cover glass, D2 represents the working distance of the microscope objective, D3 represents the air gap between the second lens group and the first lens group, and D4 represents the air gap between the second lens group and the third lens group.

[0137] As shown in Table 1-2, in the microscope objective optical system with adjustable glass cover thickness in this embodiment, the focal length of the first lens group 71 is the combined focal length of the first lens 711 to the third lens 713, i.e., f1 is 8.936; the focal length of the second lens group 72 is the combined focal length of the fourth lens 721 to the fifth lens 722, i.e., f2 is 34.107; the focal length of the third lens group 73 is the combined focal length of the sixth lens 731 to the ninth lens 734, i.e., f3 is -40.755; and the focal length of the fourth lens group 74 is the combined focal length of the tenth lens 741 to the twelfth lens 743, i.e., f4 is 33.061.

[0138] From the above, we can obtain the following values: |f2 / f1| is 3.82, |f3 / f1| is 4.56, |f3 / f2| is 1.19, |f4 / f1| is 3.70, |f4 / f2| is 0.97, |f4 / f3| is 0.81, f11 / f1 is 1.39, f12 / f1 is 1.18, f13 / f1 is -3.51, f21 / f2 is -0.61, f22 / f2 is 0.40, and f31 / f... With f32 / f3 at -0.40, f33 / f3 at 0.36, f34 / f3 at -0.32, f41 / f4 at 0.19, f42 / f4 at 0.22, f43 / f4 at -0.43, and f44 / f4 at -0.26, the objective lens optical system within the limited focal length range has a large positive refractive power. This allows the high-magnification microscope objective with adjustable cover glass thickness to have a large numerical aperture, while also enabling better control of distortion and field curvature.

[0139] Table 1-1

[0140] radius of curvature Thickness / Gap Refractive index Abbe number focal length surface unlimited 0.00 1.00 Glass cover First page unlimited D1 1.52 59.50 unlimited Second side unlimited D2 First lens First page 33.29 2.00 1.90 46.40 12.41 Second side 8.65 0.20 Second lens First page -38.45 4.20 1.50 81.60 10.55 Second side 5.87 0.00 Third lens First page 5.87 3.80 1.61 42.10 -31.34 Second side 10.54 D3 Fourth lens First page -49.31 1.10 1.71 59.00 -20.78 Second side -11.33 0.00 Fifth lens First page -11.33 4.40 1.50 81.60 13.63 Second side 14.77 D4 Sixth lens First page -37.89 3.80 1.44 94.90 16.48 Second side 8.69 0.00 Seventh Lens First page 8.69 1.10 1..72 33.80 -14.81 Second side 47.86 0.20 Eighth lens First page -7.94 4.80 1.50 81.60 13.09 Second side 29.26 0.00 Ninth Lens First page 29.26 2.40 1.61 45.40 -7.59 Second side -5.75 3.50 Tenth Lens First page -8.52 3.80 1.80 35.70 7.34 Second side 15.51 0.00 Eleventh Lens First page 15.51 1.00 1.70 56.20 -14.35 Second side -29.25 3.10 The Twelfth Lens First page 9.64 1.00 1.52 64.20 -8.76 Second side -8.90

[0141] Table 1-2

[0142] Thickness / Gap Thickness / Gap Thickness / Gap Thickness / Gap D1 0.0 0.5 1.0 1.5 D2 3.608 3.298 2.990 2.683 D3 2.300 1.761 1.171 0.527 D4 0.200 0.739 1.329 1.973

[0143] In this embodiment, T11 / T12 is 0.48, T12 / T13 is 1.11, T21 / T22 is 0.25, T31 / T32 is 3.45, T32 / T33 is 0.23, T33 / T34 is 2.00, T41 / T42 is 3.80, and T42 / T43 is 1.00.

[0144] Figures 2 to 13The figure shows the aberration diagrams of the high-magnification microscope objective optical system with adjustable glass cover thickness under different glass cover thicknesses, illustrating its aberration characteristics. When the aberration is relatively small, a higher quality imaging effect can be obtained.

[0145] Specifically, Figure 2 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness in Embodiment 1 of the present invention, when the cover glass thickness D1 is 0 mm. Figure 2 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0146] Figure 3 This is the field curve of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention when the glass cover thickness D1 is 0 mm. Figure 3 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0147] Figure 4 This is a distortion diagram of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 1 of the present invention when the cover glass thickness D1 is 0 mm. Figure 4 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0148] Figure 5 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness (D1) of Embodiment 1 of the present invention, when the cover glass thickness is 0.5 mm. Figure 5 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0149] Figure 6The image shows the field curvature of the optical system of the high-magnification microscope objective with adjustable cover glass thickness in Embodiment 1 of the present invention when the cover glass thickness D1 is 0.5 mm. Figure 6 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0150] Figure 7 The image shows the distortion of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 1 of the present invention when the cover glass thickness D1 is 0.5 mm. Figure 7 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0151] Figure 8 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness (D1) of Embodiment 1 of the present invention, when the cover glass thickness is 1 mm. Figure 8 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0152] Figure 9 This is the field curve of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 1 of the present invention, when the glass cover thickness D1 is 1 mm. Figure 9 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0153] Figure 10 This is a distortion diagram of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 1 of the present invention, when the cover glass thickness D1 is 1 mm. Figure 10As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0154] Figure 11 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness (D1) of Embodiment 1 of the present invention, when the cover glass thickness is 1.5 mm. Figure 11 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0155] Figure 12 This is the field curve of the optical system of the high-magnification microscope objective with adjustable cover glass thickness in Embodiment 1 of the present invention, when the cover glass thickness D1 is 1.5 mm. Figure 12 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0156] Figure 13 The image shows the distortion of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 1 of the present invention when the cover glass thickness D1 is 1.5 mm. Figure 13 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0157] According to another aspect of the present invention, an image acquisition device is provided, comprising an optical system with an adjustable-thickness high-magnification microscope objective, for acquiring sample images. Specifically, the specific structure and parameter settings of the adjustable-thickness high-magnification microscope objective are the same as described above, and will not be repeated here.

[0158] Example 2

[0159] like Figure 14As shown, the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 has a similar structure to that in Embodiment 1, also including a first lens group 71, a second lens group 72, a third lens group 73, and a fourth lens group 74. Specifically, the first lens group 71 includes a first lens 711, a second lens 712, and a third lens 713; the second lens group 72 includes a fourth lens 721 and a fifth lens 722; the third lens group 73 includes a sixth lens 731, a seventh lens 732, an eighth lens 733, and a ninth lens 734; and the fourth lens group 74 includes a tenth lens 741, an eleventh lens 742, and a twelfth lens 743. The optical parameters of each lens differ slightly from those in Embodiment 1.

[0160] Specifically, in the high-magnification microscope objective optical system with adjustable glass cover thickness in this embodiment two, the field of view is 25 mm and the numerical aperture (NA) is 0.57.

[0161] The first lens 711 has a focal length f11 of 12.39, a refractive index N11 of 1.90, an Abbe number V11 of 46.4, and a thickness T11 of 2.0.

[0162] The second lens 712 has a focal length f12 of 10.56, a refractive index N12 of 1.50, an Abbe number V12 of 81.6, and a thickness T12 of 4.2.

[0163] The third lens 713 has a focal length f13 of -31.35, a refractive index N13 of 1.61, an Abbe number V13 of 42.1, and a thickness T21 of 3.8.

[0164] The fourth lens 721 has a focal length f21 of -20.73, a refractive index N21 of 1.71, an Abbe number V21 of 59.0, and a thickness T22 of 1.1.

[0165] The fifth lens 722 has a focal length f22 of 13.61, a refractive index N22 of 1.50, an Abbe number V22 of 81.6, and a thickness T31 of 4.4.

[0166] The sixth lens 731 has a focal length f31 of 16.48, a refractive index N31 of 1.44, an Abbe number V31 of 94.9, and a thickness T32 of 3.8.

[0167] The seventh lens 732 has a focal length f32 of -14.80, a refractive index N32 of 1.72, an Abbe number V32 of 33.8, and a thickness T33 of 1.1.

[0168] The eighth lens 733 has a focal length f33 of 13.00, a refractive index N33 of 1.50, an Abbe number V33 of 81.6, and a thickness T34 of 4.8.

[0169] The ninth lens 734 has a focal length f34 of -7.55, a refractive index N34 of 1.61, an Abbe number V34 of 45.4, and a thickness T35 of 2.4.

[0170] The tenth lens 741 has a focal length f41 of 7.27, a refractive index N41 of 1.80, an Abbe number V41 of 35.7, and a thickness T36 of 3.8.

[0171] The eleventh lens 742 has a focal length f42 of -14.08, a refractive index N42 of 1.70, an Abbe number V42 of 56.2, and a thickness T36 of 1.0.

[0172] The twelfth lens 743 has a focal length f43 of -8.73, a refractive index N43 of 1.52, an Abbe number V43 of 64.2, and a thickness T36 of 1.0.

[0173] Other optical parameters of the objective optical system are shown in Tables 2-1 and 2-2. In Table 2-2, D1 represents the thickness of the microscope objective glass cover plate, D2 represents the working distance of the microscope objective, D3 represents the air gap between the second lens group and the first lens group, and D4 represents the air gap between the second lens group and the third lens group.

[0174] As shown in Table 2-1, in the microscope objective optical system with adjustable glass cover thickness in this embodiment, the focal length of the first lens group 71 is the combined focal length of the first lens 711 to the third lens 713, i.e., f1 is 8.932; the focal length of the second lens group 72 is the combined focal length of the fourth lens 721 to the fifth lens 722, i.e., f2 is 34.072; the focal length of the third lens group 73 is the combined focal length of the sixth lens 731 to the ninth lens 734, i.e., f3 is -40.819; and the focal length of the fourth lens group 74 is the combined focal length of the tenth lens 741 to the twelfth lens 743, i.e., f4 is 33.101.

[0175] Table 2-1

[0176] radius of curvature Thickness / Gap Refractive index Abbe number focal length surface unlimited 0.00 1.00 Glass cover First page unlimited D1 1.52 59.50 unlimited Second side unlimited D2 First lens First page 33.53 2.00 1.90 46.40 12.39 Second side 8.66 0.20 Second lens First page -38.43 4.20 1.50 81.60 10.56 Second side 5.88 0.00 Third lens First page 5.88 3.80 1.61 42.10 -31.35 Second side 10.55 D3 Fourth lens First page -49.22 1.10 1.71 59.00 -20.73 Second side -11.31 0.00 Fifth lens First page -11.31 4.40 1.50 81.60 13.61 Second side 14.75 D4 Sixth lens First page -38.00 3.80 1.44 94.90 16.48 Second side 8.65 0.00 Seventh Lens First page 8.65 1.10 1.72 33.80 -14.80 Second side 47.80 0.20 Eighth lens First page -7.93 4.80 1.50 81.60 13.00 Second side 28.30 0.00 Ninth Lens First page 28.30 2.40 1.61 45.40 -7.55 Second side -5.76 3.50 Tenth Lens First page -8.53 3.80 1.80 35.70 7.27 Second side 15.05 0.00 Eleventh Lens First page 15.05 1.00 1.70 56.20 -14.08 Second side -29.33 3.10 The Twelfth Lens First page 9.47 1.00 1.52 64.20 -8.73 Second side -8.99

[0177] Table 2-2

[0178] Thickness / Gap Thickness / Gap Thickness / Gap Thickness / Gap D1 0 0.5 1 1.5 D2 3.606 3.296 2.988 2.682 D3 2.300 1.760 1.170 0.526 D4 0.200 0.740 1.330 1.974

[0179] From the above, we can see that |f2 / f1| is 3.81, |f3 / f1| is 4.57, |f3 / f2| is 1.20, |f4 / f1| is 3.71, |f4 / f2| is 0.97, |f4 / f3| is 0.81, f11 / f1 is 1.39, f12 / f1 is 1.18, f13 / f1 is -3.51, f21 / f2 is -0.61, f22 / f2 is 0.40, and f31 / f... With f32 / f3 at -0.40, f33 / f3 at 0.36, f34 / f3 at -0.19, f41 / f4 at 0.22, f42 / f4 at -0.43, and f43 / f4 at -0.26, the objective lens optical system within the limited focal length range has a large positive refractive power, enabling this high-magnification microscope objective with adjustable cover glass thickness to have a large numerical aperture. At the same time, it can also better control distortion and field curvature.

[0180] In this embodiment, T11 / T12 is 0.48, T12 / T13 is 1.11, T21 / T22 is 0.25, T31 / T32 is 3.45, T32 / T33 is 0.23, T33 / T34 is 2.00, T41 / T42 is 3.80, and T42 / T43 is 1.00.

[0181] Figures 15 to 26 Example 2 shows aberration diagrams of the high-magnification microscope objective optical system with adjustable glass cover thickness under different glass cover thicknesses, demonstrating its aberration characteristics. When the aberration is relatively small, a higher quality imaging effect can be obtained.

[0182] Specifically, Figure 15 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness in Embodiment 2 of the present invention, when the cover glass thickness D1 is 0 mm. Figure 15 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0183] Figure 16 This is the field curve of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention when the glass cover thickness D1 is 0 mm. Figure 16 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0184] Figure 17 This is a distortion diagram of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 2 of the present invention when the cover glass thickness D1 is 0 mm. Figure 17 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0185] Figure 18 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness (D1) of Embodiment 2 of the present invention, when the cover glass thickness is 0.5 mm. Figure 18 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0186] Figure 19 This is the field curve of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention, when the glass cover thickness D1 is 0.5 mm. Figure 19 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0187] Figure 20 The image shows the distortion of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 2 of the present invention when the cover glass thickness D1 is 0.5 mm. Figure 20 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0188] Figure 21 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness (D1) of 1 mm, as shown in Embodiment 2 of the present invention. Figure 21As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0189] Figure 22 This is the field curve of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 2 of the present invention, when the glass cover thickness D1 is 1 mm. Figure 22 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0190] Figure 23 This is a distortion diagram of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 2 of the present invention when the cover glass thickness D1 is 1 mm. Figure 23 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0191] Figure 24 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness (D1) of 1.5 mm, as shown in Embodiment 2 of the present invention. Figure 24 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0192] Figure 25 This is the field curve of the optical system of the high-magnification microscope objective with adjustable cover glass thickness in Embodiment 2 of the present invention, when the cover glass thickness D1 is 1.5 mm. Figure 25As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0193] Figure 26 This is a distortion diagram of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 2 of the present invention, when the cover glass thickness D1 is 1.5 mm. Figure 26 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0194] According to another aspect of the present invention, an image acquisition device is provided, comprising an optical system with an adjustable-thickness high-magnification microscope objective, for acquiring sample images. Specifically, the specific structure and parameter settings of the adjustable-thickness high-magnification microscope objective are the same as described above, and will not be repeated here.

[0195] Example 3

[0196] like Figure 27 As shown, the structure of the high-magnification microscope objective optical system with adjustable glass cover thickness in Embodiment 3 is similar to that in Embodiment 1, also including a first lens group 71, a second lens group 72, a third lens group 73, and a fourth lens group 74. Specifically, the first lens group 71 includes a first lens 711, a second lens 712, and a third lens 713; the second lens group 72 includes a fourth lens 721 and a fifth lens 722; the third lens group 73 includes a sixth lens 731, a seventh lens 732, an eighth lens 733, and a ninth lens 734; and the fourth lens group 74 includes a tenth lens 741, an eleventh lens 742, and a twelfth lens 743. The optical parameters of each lens differ slightly from those in Embodiment 1.

[0197] Specifically, in the high-magnification microscope objective optical system with adjustable glass cover thickness in this embodiment three, the field of view is 25 mm and the numerical aperture (NA) is 0.57.

[0198] The first lens 711 has a focal length f11 of 12.38, a refractive index N11 of 1.90, an Abbe number V11 of 46.4, and a thickness T11 of 2.00.

[0199] The second lens 712 has a focal length f12 of 10.56, a refractive index N12 of 1.50, an Abbe number V12 of 81.6, and a thickness T12 of 4.2.

[0200] The third lens 713 has a focal length f13 of -31.30, a refractive index N13 of 1.61, an Abbe number V13 of 42.1, and a thickness T21 of 3.8.

[0201] The fourth lens 721 has a focal length f21 of -20.80, a refractive index N21 of 1.71, an Abbe number V21 of 59.0, and a thickness T22 of 1.1.

[0202] The fifth lens 722 has a focal length f22 of 13.62, a refractive index N22 of 1.50, an Abbe number V22 of 81.6, and a thickness T31 of 4.4.

[0203] The sixth lens 731 has a focal length f31 of 16.45, a refractive index N31 of 1.44, an Abbe number V31 of 94.9, and a thickness T32 of 3.8.

[0204] The seventh lens 732 has a focal length f32 of -14.79, a refractive index N32 of 1.72, an Abbe number V32 of 33.8, and a thickness T33 of 1.1.

[0205] The eighth lens 733 has a focal length f33 of 12.98, a refractive index N33 of 1.50, an Abbe number V33 of 81.6, and a thickness T34 of 4.8.

[0206] The ninth lens 734 has a focal length f34 of -7.56, a refractive index N34 of 1.61, an Abbe number V34 of 45.4, and a thickness T35 of 2.4.

[0207] The tenth lens 741 has a focal length f41 of 7.25, a refractive index N41 of 1.80, an Abbe number V41 of 35.7, and a thickness T36 of 3.8.

[0208] The eleventh lens 742 has a focal length f42 of -14.01, a refractive index N42 of 1.70, an Abbe number V42 of 56.2, and a thickness T36 of 1.0.

[0209] The twelfth lens 743 has a focal length f43 of -8.71, a refractive index N43 of 1.52, an Abbe number V43 of 64.2, and a thickness T36 of 1.0.

[0210] Other optical parameters of the high-magnification microscope objective optical system with adjustable glass cover thickness in this embodiment are shown in Tables 3-1 and 3-2.

[0211] Table 3-1

[0212] radius of curvature Thickness / Gap Refractive index Abbe number focal length surface unlimited 0.00 1.00 Glass cover First page unlimited D1 1.52 59.50 unlimited Second side unlimited D2 First lens First page 34.18 2.00 1.90 46.40 12.38 Second side 8.68 0.20 Second lens First page -38.19 4.20 1.50 81.60 10.56 Second side 5.88 0.00 Third lens First page 5.88 3.80 1.61 42.10 -31.30 Second side 10.58 2.30 Fourth lens First page -48.69 1.10 1.71 59.00 -20.80 Second side -11.31 0.00 Fifth lens First page -11.31 4.40 1.50 81.60 13.62 Second side 14.79 0.20 Sixth lens First page -37.72 3.80 1.44 94.60 16.45 Second side 8.68 0.00 Seventh Lens First page 8.68 1.10 1.72 33.80 -14.79 Second side 47.72 0.20 Eighth lens First page -7.93 4.80 1.50 81.60 12.98 Second side 28.15 0.00 Ninth Lens First page 28.15 2.40 1.61 45.40 -7.56 Second side -5.77 3.51 Tenth Lens First page -8.53 3.80 1.80 35.70 7.25 Second side 14.93 0.00 Eleventh Lens First page 14.93 1.00 1.70 56.20 -14.01 Second side -29.32 3.10 The Twelfth Lens First page 9.32 1.00 1.52 64.20 -8.71 Second side -9.09

[0213] Table 3-2

[0214] Thickness / Gap Thickness / Gap Thickness / Gap Thickness / Gap D1 0 0.5 1 1.5 D2 3.602 3.293 2.985 2.679 D3 2.300 1.763 1.176 0.536 D4 0.200 0.737 1.324 1.964

[0215] In Table 3-2, D1 represents the thickness of the microscope objective glass cover plate, D2 represents the working distance of the microscope objective, D3 represents the air gap between the second lens group and the first lens group, and D4 represents the air gap between the second lens group and the third lens group.

[0216] As can be seen from the above, in the microscope objective optical system with adjustable glass cover thickness in this embodiment, the focal length of the first lens group 71 is the combined focal length of the first lens 711 to the third lens 713, i.e., f1 is 8.928; the focal length of the second lens group 72 is the combined focal length of the fourth lens 721 to the fifth lens 722, i.e., f2 is 34.019; the focal length of the third lens group 73 is the combined focal length of the sixth lens 731 to the ninth lens 734, i.e., f3 is -41.248; and the focal length of the fourth lens group 74 is the combined focal length of the tenth lens 741 to the twelfth lens 743, i.e., f4 is 33.434.

[0217] From the above, we can see that |f2 / f1| is 3.81, |f3 / f1| is 4.62, |f3 / f2| is 1.21, |f4 / f1| is 3.74, |f4 / f2| is 0.98, |f4 / f3| is 0.81, f11 / f1 is 1.39, f12 / f1 is 1.18, f13 / f1 is -3.51, f21 / f2 is -0.61, f22 / f2 is 0.40, and f31 / f... With f32 / f3 at -0.40, f33 / f3 at -0.31, f34 / f3 at 0.18, f41 / f4 at 0.22, f42 / f4 at -0.42, and f43 / f4 at -0.26, the objective lens optical system within the limited focal length range has a large positive refractive power, enabling this high-magnification microscope objective with adjustable cover glass thickness to have a large numerical aperture. At the same time, it can also better control distortion and field curvature.

[0218] In Example 3, T11 / T12 is 0.48, T12 / T13 is 1.11, T21 / T22 is 0.25, T31 / T32 is 3.45, T32 / T33 is 0.23, T33 / T34 is 2.00, T41 / T42 is 3.80, and T42 / T43 is 1.00.

[0219] Figures 28 to 39 Example 3 shows aberration diagrams of the high-magnification microscope objective optical system with adjustable glass cover thickness under different glass cover thicknesses, demonstrating its aberration characteristics. When the aberration is relatively small, a higher quality imaging effect can be obtained.

[0220] Specifically, Figure 28 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention, when the glass cover thickness D1 is 0 mm. Figure 28 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0221] Figure 29 This is the field curve of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention when the glass cover thickness D1 is 0 mm. Figure 29 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0222] Figure 30 This is a distortion diagram of the optical system of the microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention when the glass cover thickness D1 is 0 mm. Figure 30 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0223] Figure 31 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness (D1) of Embodiment 3 of the present invention, when the cover glass thickness is 0.5 mm. Figure 31 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0224] Figure 32 This is the field curve of the optical system of the high-magnification microscope objective with adjustable glass cover thickness in Embodiment 3 of the present invention, when the glass cover thickness D1 is 0.5 mm. Figure 32As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0225] Figure 33 The image shows the distortion of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 3 of the present invention when the cover glass thickness D1 is 0.5 mm. Figure 33 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0226] Figure 34 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness (D1) of Embodiment 3 of the present invention, when the cover glass thickness is 1 mm. Figure 34 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0227] Figure 35 This is the field curve of the optical system of the high-magnification microscope objective with adjustable glass cover thickness (D1) of embodiment three of the present invention, when the glass cover thickness is 1 mm. Figure 35 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0228] Figure 36 This is a distortion diagram of the optical system of the microscope objective with adjustable cover glass thickness in Embodiment 3 of the present invention when the cover glass thickness D1 is 1 mm. Figure 36 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0229] Figure 37 This is a spherical aberration diagram of the optical system of the high-magnification microscope objective with adjustable cover glass thickness (D1) of Embodiment 3 of the present invention, when the cover glass thickness is 1.5 mm. Figure 37 As shown, the horizontal axis represents spherical aberration in mm, and the vertical axis represents the normalized field of view in 1. The solid line represents the F line, the dashed line represents the d line, and the single-dot dashed line represents the C line. The spherical aberration of this microscope objective optical system with adjustable cover plate thickness is controlled within ±0.004 mm, resulting in optimal center resolution for the microscope objective optical system with adjustable cover plate thickness.

[0230] Figure 38 This is the field curve of the optical system of the high-magnification microscope objective with adjustable glass cover thickness (D1) of embodiment three of the present invention, when the glass cover thickness is 1.5 mm. Figure 38 As shown, the horizontal axis represents the object plane movement in mm, and the vertical axis represents the image height in mm. Solid lines represent meridional rays of various wavelengths, and dashed lines represent sagittal rays of various wavelengths. The field curvature distribution shows that the field curvature of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.0015 mm, ensuring clear imaging within the field of view.

[0231] Figure 39 This is a distortion diagram of the optical system of the microscope objective with adjustable cover glass thickness (D1) of Embodiment 3 of the present invention when the cover glass thickness is 1.5 mm. Figure 39 As shown, the horizontal axis represents distortion (%), and the vertical axis represents image height (mm). The distortion distribution indicates that the distortion of this high-magnification microscope objective optical system with adjustable cover plate thickness is controlled within ±0.2%, effectively controlling imaging deformation within the field of view.

[0232] According to another aspect of the present invention, an image acquisition device is provided, comprising an optical system with an adjustable-thickness high-magnification microscope objective, for acquiring sample images. Specifically, the specific structure and parameter settings of the adjustable-thickness high-magnification microscope objective are the same as described above, and will not be repeated here.

[0233] The above are merely specific embodiments of the present invention, but the design concept of the present invention is not limited thereto. Any non-substantial modifications made to the present invention using this concept shall be considered as infringing upon the protection scope of the present invention.

Claims

1. A high-magnification microscope objective with adjustable glass cover thickness, characterized in that, It consists of a first lens group with positive refractive power, a second lens group with positive refractive power, a third lens group with negative refractive power, and a fourth lens group with positive refractive power, arranged sequentially from the object plane to the image plane. The first lens group is closest to the object, and the first lens, the second lens, and the third lens are arranged sequentially along the direction from the object plane to the image plane; the first lens has positive refractive power, the second lens has positive refractive power, and the third lens has negative refractive power; The high-magnification microscope objective with adjustable glass cover plate thickness meets the following conditions: 0.5 < |f2 / f1| < 6.0; 0.6 < |f3 / f1| < 7.0; 0.1 < |f3 / f2| < 3.8; 0.5 < |f4 / f1| < 8.0; 0.1 < |f4 / f2| < 3.5; 0.1 < |f4 / f3| < 3.2; Where f1 is the focal distance of the first lens group, f2 is the focal distance of the second lens group, f3 is the focal distance of the third lens group, and f4 is the focal distance of the fourth lens group.

2. The high-magnification microscope objective with adjustable glass cover plate thickness according to claim 1, characterized in that, The second lens group has a fourth lens and a fifth lens arranged sequentially along the direction from the object plane to the image plane; the second lens group is a focusing group, which adapts to the change in the thickness of the glass cover by changing the air gap between the second lens group and the first and third lens groups; the fourth lens has negative refractive power and the fifth lens has positive refractive power.

3. The high-magnification microscope objective with adjustable glass cover plate thickness according to claim 2, characterized in that, The third lens group comprises a sixth lens, a seventh lens, an eighth lens, and a ninth lens arranged sequentially along the direction from the object plane to the image plane; the sixth lens has positive refractive power, the seventh lens has negative refractive power, the eighth lens has positive refractive power, and the ninth lens has negative refractive power.

4. The high-magnification microscope objective with adjustable glass cover plate thickness according to claim 3, characterized in that, The fourth lens group comprises a tenth lens, an eleventh lens, and a twelfth lens arranged sequentially along the direction from the object plane to the image plane; the tenth lens has positive refractive power, the eleventh lens has negative refractive power, and the twelfth lens has negative refractive power.

5. The high-magnification microscope objective with adjustable glass cover plate thickness according to claim 4, characterized in that, The first lens group, the second lens group, the third lens group, and the fourth lens group satisfy the following condition: 0.1 <f11 / f1<3.5; 0.1 <f12 / f1<3.2; -6.0 <f13 / f1<-0.2; -2.0 <f21 / f2<-0.2; 0.1 <f22 / f2<1.2; -1.2 <f31 / f3<-0.1; 0.05 <f32 / f3<1.5; -2.0 <f33 / f3<0; 0 <f34 / f3<1.0; 0 <f41 / f4<1.2; -2.5 <f42 / f4<-0.1; -2.5 <f43 / f4<0; Wherein, f11 is the focal distance of the first lens, f12 is the focal distance of the second lens, f13 is the focal distance of the third lens, f21 is the focal distance of the fourth lens, f22 is the focal distance of the fifth lens, f31 is the focal distance of the sixth lens, f32 is the focal distance of the seventh lens, f33 is the focal distance of the eighth lens, f34 is the focal distance of the ninth lens, f41 is the focal distance of the tenth lens, f42 is the focal distance of the eleventh lens, and f43 is the focal distance of the twelfth lens.

6. The high-magnification microscope objective with adjustable glass cover plate thickness according to claim 4, characterized in that, The refractive indices of the first lens, second lens, third lens, fourth lens, fifth lens, sixth lens, seventh lens, eighth lens, ninth lens, tenth lens, eleventh lens, and twelfth lens satisfy the following condition: 1.90≤N11≤2.0； 1.40≤N12≤1.50； 1.40≤N13≤1.61； 1.50≤N21≤1.71； 1.30≤N22≤1.50； 1.30≤N31≤1.44； 1.50≤N32≤1.72； 1.50≤N33≤1.7； 1.61≤N34≤1.7； 1.60≤N41≤1.80； 1.60≤N42≤1.70； 1.30≤N43≤1.52； Wherein, N11 is the refractive index of the first lens, N12 is the refractive index of the second lens, N13 is the refractive index of the third lens, N21 is the refractive index of the fourth lens, N22 is the refractive index of the fifth lens, N31 is the refractive index of the sixth lens, N32 is the refractive index of the seventh lens, N33 is the refractive index of the eighth lens, N34 is the refractive index of the ninth lens, N41 is the refractive index of the tenth lens, N42 is the refractive index of the eleventh lens, and N43 is the refractive index of the twelfth lens.

7. The high-magnification microscope objective with adjustable glass cover plate thickness according to claim 4, characterized in that, The Abbe numbers of the first lens, second lens, third lens, fourth lens, fifth lens, sixth lens, seventh lens, eighth lens, ninth lens, tenth lens, eleventh lens, and twelfth lens satisfy the following condition: 40≤V11≤46.4； 81.6≤V12≤90； 35≤V13≤42.1； 59.0≤V21≤65； 81.6≤V22≤90； 90≤V31≤94.9； 33.8≤V32≤40； 75≤V33≤81.6； 40≤V34≤45.4； 35.7≤V41≤40； 56.2≤V42≤65； 64.2≤V43≤70； Wherein, V11 is the Abbe number of the first lens, V12 is the Abbe number of the second lens, V13 is the Abbe number of the third lens, V21 is the Abbe number of the fourth lens, V22 is the Abbe number of the fifth lens, V31 is the Abbe number of the sixth lens, V32 is the Abbe number of the seventh lens, V33 is the Abbe number of the eighth lens, V34 is the Abbe number of the ninth lens, V41 is the Abbe number of the tenth lens, V42 is the Abbe number of the eleventh lens, and V43 is the Abbe number of the twelfth lens.

8. The high-magnification microscope objective with adjustable glass cover plate thickness according to claim 4, characterized in that, The thicknesses of the first lens, second lens, third lens, fourth lens, fifth lens, sixth lens, seventh lens, eighth lens, ninth lens, tenth lens, eleventh lens, and twelfth lens satisfy the following conditions: 0.1 <T11 / T12<0.8; 0.5 <T12 / T13<1.5; 0.05 <T21 / T22<0.6; 1.0 <T31 / T32<5.0; 0.05 <T32 / T33<0.5; 0.5 <T33 / T34<5.0; 1.5 <T41 / T42<7.5; 0.4 <T42 / T43<4.0; Wherein, T11 is the thickness of the first lens on the optical axis, T12 is the thickness of the second lens on the optical axis, T13 is the thickness of the third lens on the optical axis, T21 is the thickness of the fourth lens on the optical axis, T22 is the thickness of the fifth lens on the optical axis, T31 is the thickness of the sixth lens on the optical axis, T32 is the thickness of the seventh lens on the optical axis, T33 is the thickness of the eighth lens on the optical axis, T34 is the thickness of the ninth lens on the optical axis, T41 is the thickness of the tenth lens on the optical axis, T42 is the thickness of the eleventh lens on the optical axis, and T43 is the thickness of the twelfth lens on the optical axis.

9. An image capturing device, characterized in that, Including a high-magnification microscope objective with adjustable glass cover plate thickness as described in any one of claims 1 to 8.

Images