Detection chip, detection device, and method for preparing and operating the detection chip

Abstract

A detection chip (100), a detection device, and a method for preparing and operating the detection chip (100). The detection chip (100) comprises: a substrate (110), the substrate (110) comprising a first surface (111); wherein the substrate (110) comprises a liquid storage chamber (112), the liquid storage chamber (112) is configured to contain liquid and comprises a bottom located on the first surface (111), the bottom comprises a central area (113) and an edge area surrounding the central area (113), the edge area comprises a channel (114 / 114') surrounding the central area, the thickness of the channel (114 / 114') is less than the thickness of the central area (113). The detection chip (100) is convenient for releasing the liquid stored therein, and has a simple structure and manufacturing process, and low cost.

Description

Technical Field

[0001] Embodiments of the present disclosure relate to a detection chip, a detection device, and a method for preparing and operating the detection chip. Background Art

[0002] Microfluidic chips, also known as lab-on-a-chip, are devices that integrate basic operating units such as sample preparation, reaction, separation, and detection in the fields of biology, chemistry, and medicine onto a chip with micron-scale microchannels to automatically complete the entire process of reaction and analysis. The advantages of analytical detection devices based on microfluidic chips include small sample usage, fast analysis speed, and ease of making portable instruments, making them very suitable for instant and on-site analysis. Summary of the invention

[0003] At least one embodiment of the present disclosure provides a detection chip, which includes:

[0004] a substrate including a first surface;

[0005] wherein the substrate comprises a liquid storage chamber, the liquid storage chamber is configured to contain liquid and comprises a bottom located on the first surface,

[0006] The bottom portion includes a central region and an edge region surrounding the central region,

[0007] The edge region includes a channel surrounding the central region, and a thickness of the channel is less than a thickness of the central region.

[0008] For example, in the detection chip according to at least one embodiment of the present disclosure, the channel is a non-closed channel to partially surround the central area.

[0009] For example, in the detection chip according to at least one embodiment of the present disclosure, the channel is a closed channel to surround the central area.

[0010] For example, in the detection chip according to at least one embodiment of the present disclosure, the liquid storage chamber is cylindrical, and the channel is substantially annular.

[0011] For example, in the detection chip according to at least one embodiment of the present disclosure, the central angle corresponding to the channel is 340 degrees to 358 degrees.

[0012] For example, in the detection chip according to at least one embodiment of the present disclosure, the thickness of the central area is 0.1 mm-3 mm.

[0013] For example, in the detection chip according to at least one embodiment of the present disclosure, the thickness of the channel is 0.05 mm-0.3 mm, and the width of the channel is 0.1 mm-3 mm.

[0014] For example, in the detection chip according to at least one embodiment of the present disclosure, the surface of the bottom facing the inner space of the liquid storage chamber is a plane, and the channel is recessed on the first surface toward the inner space of the liquid storage chamber.

[0015] For example, in the detection chip according to at least one embodiment of the present disclosure, the surface of the bottom away from the internal space of the liquid storage chamber is a plane, and the channel is recessed away from the internal space of the liquid storage chamber at the surface of the bottom facing the internal space of the liquid storage chamber.

[0016] For example, in the detection chip according to at least one embodiment of the present disclosure, the liquid storage chamber further includes a side wall, and the channel is between the side wall and the central area.

[0017] The edge region also includes a connecting portion,

[0018] The connecting portion is between the side wall and the central area, and corresponds to an opening portion of the channel that does not surround the central area.

[0019] For example, in the detection chip according to at least one embodiment of the present disclosure, the connection portion is configured to allow the central region to form an angle greater than zero degrees and less than 25 degrees with the sidewall when the channel is destroyed.

[0020] For example, in the detection chip according to at least one embodiment of the present disclosure, the substrate further includes a liquid flow channel on the first surface.

[0021] Wherein, the liquid flow channel is connected with the channel.

[0022] For example, in the detection chip according to at least one embodiment of the present disclosure, on the first surface, the connection portion and the liquid flow channel are located on opposite sides of the central area.

[0023] For example, the detection chip according to at least one embodiment of the present disclosure further includes: a first elastic membrane layer,

[0024] wherein the liquid flow channel comprises a groove on the first surface of the substrate,

[0025] The first elastic film layer is on the first surface of the substrate and covers the bottom and the groove to provide a space for liquid flow.

[0026] For example, the detection chip according to at least one embodiment of the present disclosure further includes a second elastic membrane layer.

[0027] The substrate further includes a second surface opposite to the first surface,

[0028] The second elastic film layer is on the second surface of the substrate and covers the opening of the liquid storage chamber on the second surface.

[0029] For example, the detection chip according to at least one embodiment of the present disclosure further includes: a first adhesive layer and a second adhesive layer,

[0030] Wherein, the first adhesive layer is between the substrate and the first elastic film layer to connect the substrate and the first elastic film layer; and

[0031] The second adhesive layer is between the substrate and the second elastic film layer to connect the substrate and the second elastic film layer.

[0032] For example, the detection chip according to at least one embodiment of the present disclosure further includes a liquid operation area.

[0033] The liquid operation area is in communication with the liquid flow channel.

[0034] For example, in the detection chip according to at least one embodiment of the present disclosure, the liquid operation area includes at least one selected from the group consisting of a liquid mixing area, a liquid detection area, and a liquid storage area.

[0035] At least one embodiment of the present disclosure further provides a detection device, which includes:

[0036] The detection chip provided by any embodiment of the present disclosure; and

[0037] The force mechanism is configured to apply a force on the bottom of the detection chip toward the internal space of the liquid storage chamber when in use, so as to destroy the channel and make the liquid contained in the internal space flow out from the bottom.

[0038] At least one embodiment of the present disclosure further provides a method for preparing a detection chip, which comprises:

[0039] providing a substrate, the substrate comprising a first surface,

[0040] The substrate includes a liquid storage chamber, the liquid storage chamber is configured to contain liquid and is included at the bottom of the first surface,

[0041] The bottom portion includes a central region and an edge region surrounding the central region, and

[0042] The edge region includes a channel surrounding the central region, and a thickness of the channel is less than a thickness of the central region.

[0043] For example, the method for preparing a detection chip according to at least one embodiment of the present disclosure further includes: preparing the substrate by an integrated molding method.

[0044] At least one embodiment of the present disclosure further provides a method for operating the detection chip provided by any embodiment of the present disclosure, comprising:

[0045] A force is applied on the bottom of the liquid storage chamber toward the inner space of the liquid storage chamber to destroy the channel and make the liquid contained in the inner space flow out from the bottom.

[0046] At least one embodiment of the present disclosure further provides a method for operating the detection chip provided by at least one embodiment of the present disclosure, comprising:

[0047] A force is applied at a position on the first elastic membrane layer corresponding to a position 0-3 / 4 of the length of the central area of ​​the connecting portion to apply a force to the bottom of the liquid storage chamber, thereby destroying the channel and causing the liquid contained in the internal space of the liquid storage chamber to flow out from the bottom. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below. Obviously, the drawings described below only relate to some embodiments of the present disclosure, but are not intended to limit the present disclosure.

[0049] Figure 1 is a schematic three-dimensional perspective view of a detection chip according to at least one embodiment of the present disclosure.

[0050] Figure 2 According to at least one embodiment of the present disclosure Figure 1 A bottom perspective view of the liquid storage chamber of the detection chip is shown.

[0051] Figure 3 According to at least one embodiment of the present disclosure Figure 1 A bottom plan view of the liquid storage chamber of the detection chip is shown.

[0052] Figure 4 According to at least one embodiment of the present disclosure Figure 2 A three-dimensional cross-sectional view of the line AA'.

[0053] Figure 5 According to at least one embodiment of the present disclosure Figure 2 Cross-sectional view along line BB'.

[0054] Figure 6 According to at least one embodiment of the present disclosure Figure 2 Another cross-sectional view of the line BB' in FIG.

[0055] Fig. 7A and Figure 7B is a diagram showing a liquid release process according to at least one embodiment of the present disclosure, along Figure 2 Cross-sectional view along line AA'.

[0056] Figure 8 is a schematic diagram of an exploded view of another detection chip according to at least one embodiment of the present disclosure.

[0057] Fig. 9 yes Figure 8 The assembly diagram of the detection chip is shown.

[0058] Fig.10 According to at least one embodiment of the present disclosure Figure 8 Schematic perspective view of a substrate in FIG.

[0059] Fig.11 According to at least one embodiment of the present disclosure Figure 8 A bottom perspective view of the substrate in FIG.

[0060] Fig. 12A and Fig. 12B is a diagram showing a liquid release process according to at least one embodiment of the present disclosure, along Fig. 9 Cross-sectional view along line AA'.

[0061] Fig.13 is a schematic block diagram of a detection device according to at least one embodiment of the present disclosure. DETAILED DESCRIPTION

[0062] In order to make the purpose, technical solution and advantages of the embodiments of the present disclosure clearer, the technical solution of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0063] Unless otherwise defined, the technical terms or scientific terms used herein should be understood by people with ordinary skills in the field to which the present invention belongs. "First", "second" and similar words used in this disclosure do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, "include" or "comprise" and similar words mean that the elements or objects appearing before the word cover the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0064] It should be understood that in at least one embodiment of the present disclosure, “substantially the same” means that the difference between two objects is within 5% to -5% of the compared objects.

[0065] The reagent storage structure in the current microfluidic chip is either complex in structure or requires complicated preparation process, which makes the cost of microfluidic chip as consumables too high. For example, in some microfluidic chips, the bottom of the reservoir is encapsulated by aluminum film or plastic film. When the aluminum film or plastic film is pierced to release the reagent stored in the reservoir, the edge of the rupture will bulge upward, which is easy to cause reagent residue, and it is impossible to completely release all the reagents in the reservoir, so that it is impossible to add enough reagents in the test, resulting in abnormal biological reaction results. In addition, in these microfluidic chips, the liquid stored in the reservoir is easy to leak, and processes such as hot pressing and laser welding are also required, resulting in complex processes and high costs.

[0066] In response to at least one of the above problems, at least one embodiment of the present disclosure provides a detection chip that is convenient for releasing liquid stored therein and has a simple structure and manufacturing process and low cost.

[0067] The detection chip provided in the embodiments of the present disclosure may be a microfluidic chip; however, it should be understood that the embodiments of the present disclosure are not limited thereto.

[0068] In addition, an embodiment of the present disclosure also provides a detection device including any of the above detection chips and a method for preparing and operating any of the above detection chips.

[0069] Figure 1 is a schematic three-dimensional perspective view of a detection chip according to at least one embodiment of the present disclosure. Figure 2 According to at least one embodiment of the present disclosure Figure 1 A bottom perspective view of the liquid storage chamber of the detection chip is shown. Figure 3 According to at least one embodiment of the present disclosure Figure 1 A bottom plan view of the liquid storage chamber of the detection chip is shown. Figure 4 is a method of implementing at least one embodiment of the present disclosure Figure 2 Cross-sectional view along line AA'. Figure 5 According to at least one embodiment of the present disclosure Figure 2 Cross-sectional view along line BB'. Figure 6 According to at least one embodiment of the present disclosure Figure 2 Another cross-sectional view of the line BB' in FIG.

[0070] like Figure 1-6 As shown, the detection chip 100 according to at least one embodiment of the present disclosure includes a substrate 110. The substrate 110 includes a first surface 111 and a second surface 111' opposite to the first surface 111. The substrate 110 also includes a liquid storage chamber 112. The liquid storage chamber 112 is configured to contain a liquid (e.g., various reagents required for analysis and detection) and is included at the bottom of the first surface 111. The bottom includes a central area 113 and an edge area surrounding the central area 113. The edge area includes a channel 114 / 114' / 114" surrounding the central area 113, and the thickness h1 of the channel 114 / 114' / 114" is less than the thickness h2 of the central area 113. It should be understood that the thickness of the channel 114 / 114' / 114" mentioned here refers to the thickness of the bottom of the liquid storage chamber 112 at the channel 114 / 114' / 114", and the thickness of the central area 113 refers to the thickness of the bottom of the liquid storage chamber 112 at the central area 113.

[0071] Since the thickness h1 of the channel 114 / 114' / 114" is less than the thickness h2 of the central area 113, when a force is applied toward the inside of the liquid storage chamber 112 at a position corresponding to the liquid storage chamber 112 (e.g., at the central area 113) on the first surface 111, the channel 114 / 114' / 114" is more easily destroyed relative to the central area 113, so that an opening is formed along the path of the channel 114 / 114' / 114", so as to release the liquid contained in the liquid storage chamber 112 from the liquid storage chamber 112, for example, to an external device connected to the detection chip 100 (e.g., other analysis and detection devices, etc.) or to a liquid flow channel in the detection chip 100. For example, When a force is applied toward the inside of the liquid storage chamber 112 at a position corresponding to the liquid storage chamber 112 on the first surface 111 (for example, at the central area 113), the bottom of the liquid storage chamber 112 is split along the channel 114 / 114' / 114", so that the edge of the central area 113 surrounded by the channel 114 is separated from the other parts of the bottom of the liquid storage chamber 112, and the force is continued to be applied to the central area 113, so that the central area 113 continues to move in a direction away from the first surface 111 (for example, tilted relative to the first surface 111), and an opening is formed between the central area 113 and the other parts of the bottom of the liquid storage chamber 112 to release the liquid contained in the liquid storage chamber 112 from the liquid storage chamber 112. In this way, the problem of not being able to completely release all reagents in the liquid storage pool in the above-mentioned other detection chips can be avoided.

[0072] In this document, thickness refers to the thickness in a direction perpendicular to the first surface 111. It should be understood that, in the case where the channel 114 / 114' / 114" has the same thickness throughout the entire circumference, the thickness of the channel 114 / 114' / 114" mentioned here may refer to the thickness at any point of the channel 114 / 114' / 114"; in the case where the channel 114 / 114' / 114" has different thicknesses throughout the circumference, the thickness of the channel 114 / 114' / 114" mentioned here refers to the maximum thickness of the channel 114 / 114' / 114". Similarly, in the case where the central area 113 has the same thickness throughout the entire area, the thickness of the central area 113 mentioned here may refer to the thickness at any point of the central area 113; in the case where the central area 113 has different thicknesses throughout the entire area, the thickness of the central area 113 mentioned here refers to the minimum thickness of the central area 113. Hereinafter, various embodiments of the present disclosure will be described by taking an example in which the central region 113 has the same thickness over the entire area and the channel 114 has the same thickness over the entire circumference.

[0073] The material of the substrate 110 can be any suitable material according to actual requirements, for example, it can be glass, silicon, quartz, ceramic, polyethylene terephthalate (PET), polystyrene (PS), polymethyl methacrylate (PMMA), polypropylene (PP), polycarbonate (PC) or a combination thereof, and the embodiments of the present disclosure are not limited to this. For example, when the detection chip 100 is used for immunoassay, the material of the substrate 110 can be PS or PMMA; when the detection chip 100 is used for molecular detection, the material of the substrate 110 can be PP or PC.

[0074] Although Figure 1-6 It is only shown that the substrate 110 includes one liquid storage chamber, however, the embodiments of the present disclosure are not limited to this. In other embodiments, according to actual requirements, the substrate 110 may include any number of liquid storage chambers, which may contain various reagents required for analysis and detection, and these liquid storage chambers may have the same or different shapes and may contain the same or different liquids.

[0075] like Figure 2 As shown, in at least one embodiment of the present disclosure, the channel 114 is a non-enclosed channel to partially surround the central area 113. For example, the channel 114 may be substantially C-shaped, U-shaped, etc.

[0076] However, it should be understood that in other embodiments, Figure 3 As shown, the channel 114' may be a closed channel to completely surround the central area 113. For example, the channel 114' may be circular or polygonal (eg, quadrilateral, etc.).

[0077] Exemplarily, the liquid storage chamber 112 may be cylindrical with a bottom surface parallel to the first surface 111, and the channel 114 / 114' / 114" may be substantially annular. However, it should be understood that in other embodiments, according to actual requirements, the liquid storage chamber 112 and the channel 114 / 114' / 114" may also be in any suitable shape, and the embodiments of the present disclosure are not limited to this. In the following, the embodiments of the present disclosure will be described by taking the example that the liquid storage chamber 112 is cylindrical and the channel 114 is annular.

[0078] In some embodiments, the central angle of the circle corresponding to the channel may be 330 degrees to 360 degrees. For example, the central angle of the circle corresponding to the channel 114 may be 340 degrees to 358 degrees. Exemplarily, the central angle of the circle corresponding to the channel 114 may be 340 degrees, 345 degrees, 350 degrees, 355 degrees, or 358 degrees. For example, the central angle of the circle corresponding to the channel 114 may be 350 degrees, so that the channel 114 is easily destroyed, thereby splitting along the path of the channel 114 to form an opening, so that the liquid contained in the liquid storage chamber 112 is completely released from the liquid storage chamber 112, and the channel 114 is prevented from being unintentionally destroyed during non-use processes such as transportation and storage.

[0079] In some embodiments, the thickness of the central area 113 may be, for example, 0.1 mm to 3 mm. For example, the thickness of the central area 113 may be 0.1 mm, 0.5 mm, 1 mm, 1.5 mm, 2 mm, 2.5 mm or 3 mm.

[0080] In some embodiments, for the above materials, the thickness of the channel 114 may be, for example, 0.05 mm to 0.3 mm. For example, the thickness of the channel 114 may be 0.05 mm, 0.1 mm, 0.15 mm, 0.2 mm, 0.25 mm, or 0.3 mm.

[0081] In some embodiments, the width of the channel 114 is 0.1 mm-3 mm. The width mentioned here refers to the width in the direction parallel to the first surface 111. Exemplarily, the width of the channel 114 can be 0.1 mm, 0.5 mm, 1 mm, 1.5 mm, 2 mm, 2.5 mm or 3 mm, so that the channel 114 is easy to be destroyed, thereby releasing the liquid contained in the liquid storage chamber 112 from the liquid storage chamber 112.

[0082] In an exemplary embodiment, the central angle of the channel 114 is 350 degrees, the width of the channel 114 is 1 mm, the thickness of the channel 114 is 0.1 mm, and the thickness of the central area 113 is 1.5 mm. In this way, the channel 114 can be easily destroyed, thereby releasing the liquid contained in the liquid storage chamber 112 from the liquid storage chamber 112.

[0083] like Figure 4 and Figure 5 As shown, in some embodiments, the surface of the bottom of the liquid storage chamber 112 facing the inner space of the liquid storage chamber 112 is a plane, and the channel 114 is recessed on the first surface 111 toward the inner space of the liquid storage chamber 112. Figure 6As shown, in other embodiments, the surface of the bottom of the liquid storage chamber 112 facing the first surface 111 is a plane, and the channel 114" is recessed on the inner bottom surface of the liquid storage chamber 112 toward the first surface 111. The embodiments of the present disclosure are not limited to this. In the following, the substrate 110 including the channel 114 recessed on the first surface 111 toward the internal space of the liquid storage chamber 112 will be described as an example.

[0084] In some embodiments, the liquid storage chamber 112 further includes a side wall 116, and the channel 114 is between the side wall 116 and the central area 113. Figure 4 As shown, the channel 114 may, for example, be in direct contact with the sidewall 116; Figure 5-7B As shown, the channel 114 may be spaced apart from the sidewall 116 , for example, but the embodiments of the present disclosure are not limited thereto.

[0085] The edge region may further include a connection portion 117. The connection portion 117 is between the sidewall 116 and the central region 113, and the connection portion 117 corresponds to the opening portion of the channel 114 that does not surround the central region 113. For example, the channel 114 and the connection portion 117 may surround the central region 113 in one full circle. Of course, it should be understood that when the substrate 110 includes Figure 3 In the case of the closed channel 114' shown, the connecting portion 117 may not exist. The embodiments of the present disclosure are not limited to this.

[0086] In some embodiments, the connection portion 117 is configured to substantially remain in its original shape and not split due to its thicker thickness when the channel 114 is damaged and split to form an opening, thereby connecting between the central area 113 and the side wall 116 and allowing the central area 113 to form an angle with the side wall 116 when it is pushed open. For example, the central area 113 can be configured to have a certain rigidity so that when it is pushed open, it can substantially remain in its original shape without bending, thereby avoiding blocking the liquid in the liquid storage chamber 112 from flowing out of the opening formed by the split of the channel 114.

[0087] For example, when the central angle corresponding to the channel 114 is 350 degrees, when a force is applied toward the inside of the liquid storage chamber 112 at a position corresponding to the liquid storage chamber 112 on the first surface 111 (for example, at the central area 113), the size of the opening formed by the splitting of the channel 114 is substantially the same as the size of the cross-section of the liquid storage chamber 112, and the central area 113 is still connected to the substrate 110 via the connection portion 117. Continuing to apply force to the central area 113 can form an angle between the central area 113 and the side wall 116 that is greater than 0 degrees and less than 90 degrees, for example, an angle of 10 degrees to 25 degrees, thereby completely releasing the liquid contained in the liquid storage chamber 112 from the liquid storage chamber 112. Furthermore, since the central area 113 is still connected to the substrate 110 via the connection portion 117, the problem of the central area 113 being completely separated from the substrate 110 and becoming horizontally placed and blocking the above-mentioned opening as the liquid in the liquid storage chamber 112 flows is avoided.

[0088] In some embodiments, the connection portion 117 is configured to allow the central area 113 to form an angle with the side wall 116 that is greater than 0 degrees and less than 90 degrees, such as an angle that is greater than 0 degrees and less than 25 degrees, so as to facilitate the release of all the liquid in the liquid storage chamber 112. For example, the central angle corresponding to the connection portion 117 can be 2 degrees to 20 degrees. Exemplarily, the central angle corresponding to the connection portion 117 is 10 degrees.

[0089] For example, when a force is applied toward the interior of the liquid storage chamber 112 on the first surface 111 at a position corresponding to the liquid storage chamber 112 (for example, in the central area 113), the connecting portion 117 bends so that the central area 113 can form an angle greater than 0 degrees and less than 90 degrees with the side wall 116, for example, an angle of 10 degrees to 25 degrees, and at this time, the bending of the connecting portion 117 can be plastic deformation, so that the central area 113 and the side wall 116 still maintain the above-mentioned angle after the force is removed, or the bending of the connecting portion 117 can be elastic deformation, so that the force needs to be applied continuously to maintain the angle between the central area 113 and the side wall 116, for example until the liquid in the liquid storage chamber 112 is completely released.

[0090] Fig. 7A and Figure 7B is a liquid release process according to at least one embodiment of the present disclosure Figure 2 A cross-sectional view taken along line AA' in FIG. Fig. 7A and Figure 7B Shows Figure 2 Schematic diagram of the liquid release process of the detection chip shown. Fig. 7A and Figure 7BAs shown, when a force is applied toward the bottom of the liquid storage chamber 112 (e.g., the central area 113) in the direction indicated by the arrow A, the channel 114 can be destroyed, the bottom of the liquid storage chamber 112 is split along the channel 114, the edge of the central area 113 surrounded by the channel 114 is separated from the other parts of the bottom of the liquid storage chamber 112, and the connecting portion 117 connects the central area 113 and the side wall 116, and the connecting portion 117 allows the angle θ between the central area 113 and the side wall 116 to be greater than 0 degrees and less than 90 degrees, such as an angle of 10 degrees to 25 degrees, so as to facilitate the complete release of the liquid in the liquid storage chamber 112. Although in Figure 4 , Fig. 7A and Figure 7B 11 and 112. It is shown that the side wall 116 is substantially perpendicular to the first surface 111. However, it should be understood that the embodiments of the present disclosure are not limited thereto. In other embodiments, the side wall 116 may be inclined relative to the first surface 111, or the side wall 116 may have a curved surface.

[0091] In some embodiments, in a direction perpendicular to the first surface 111, the thickness of the connection portion 117 may be substantially the same as the thickness of the central area 113 to simplify the manufacturing process, however, it should be understood that the embodiments of the present disclosure are not limited thereto. For example, in other embodiments, the thickness of the connection portion 117 may be less than the thickness of the central area 113. In addition, the thickness of the connection portion 117 is greater than the thickness of the channel 114, so that when the channel 114 is damaged, the connection portion 117 can still be connected to the central area 113 and the sidewall 116.

[0092] It should be understood that in at least one embodiment of the present disclosure, the thickness of the connecting portion 117 may be substantially the same as the thickness of the central area 113, which may mean that the thickness of the connecting portion 117 and the thickness of the central area 113 are the same, substantially the same, or the difference is within a preset range, for example, the difference between the thickness of the connecting portion 117 and the thickness of the central area 113 is within 5% to -5% of the compared thickness.

[0093] Figure 8 is a schematic diagram of an exploded view of another detection chip according to at least one embodiment of the present disclosure. Fig. 9 yes Figure 8 The assembly diagram of the detection chip is shown in FIG. Figure 8 As shown, the detection chip 200 according to at least one embodiment of the present disclosure may include a substrate 210. The substrate 210 is substantially the same as the substrate 110 described in any of the above embodiments, except that the substrate 210 further includes a liquid flow channel 215 on the first surface 211. For the description of the same or similar parts of the substrate 210 and the substrate 110, please refer to the specific description of each embodiment above, and will not be repeated herein.

[0094] Fig.10 According to at least one embodiment of the present disclosure Figure 8 Schematic perspective view of a substrate in FIG. Fig.11 According to at least one embodiment of the present disclosure Figure 8 A bottom-up stereogram of the substrate in FIG. Figure 10-11 As shown, in at least one embodiment, the substrate 210 includes a liquid flow channel 215 on the first surface 211. The liquid flow channel 215 may include a groove on the first surface 211 of the substrate 210. For example, the groove may be formed by any suitable process such as laser engraving, photolithography, or may be formed integrally with the substrate 210 during the process of forming and preparing the substrate 210, and the embodiments of the present disclosure are not limited to this. The liquid flow channel 215 is used to transport the liquid released from the liquid storage chamber 212 to other parts of the detection chip 200, such as the liquid operation area 219, including but not limited to the liquid mixing area, the liquid detection area, the liquid storage area and the membrane pump area.

[0095] In the case where the bottom of the liquid storage chamber 212 includes a channel 214 that is recessed from the first surface 211 toward the inner space of the liquid storage chamber 112 , the liquid flow channel 215 may be in communication with the channel 214 .

[0096] In some embodiments, Figure 10-11 As shown, on the first surface 211, the connection portion 217 and the liquid flow channel 215 are located on opposite sides of the central area 213, so that the liquid released from the liquid storage chamber 212 can enter the liquid flow channel 215. However, it should be understood that in other embodiments, the connection portion 217 and the liquid flow channel 215 can be positioned arbitrarily relative to each other, and the embodiments of the present disclosure are not limited to this. For example, the extension direction of the connection portion 217 can be 45 degrees, 90 degrees, 135 degrees, etc. with the extension direction of the liquid flow channel 215.

[0097] like Figure 8 As shown, in addition to the above-mentioned substrate 210, the detection chip 200 according to at least one embodiment of the present disclosure may further include a first elastic film layer 220. The substrate 210 includes a first surface 211 and a second surface 211' opposite to the first surface 211. In the direction from the first surface 211 to the second surface 211', the first elastic film layer 220 is below the first surface 211 of the substrate 210. For example, the first elastic film layer 220 may be liquid-tightly connected to the substrate 210. The first elastic film layer 220 covers at least the bottom of the liquid storage chamber 212. In some embodiments, in addition to covering the bottom of the liquid storage chamber 212, the first elastic film layer 220 also covers the groove of the liquid flow channel 215, thereby closing the open end face of the groove of the liquid flow channel 215 parallel to the first surface 211 to provide a space for liquid flow, for example, also forming a space for reagent reaction. In some embodiments, the first elastic film layer 220 may also cover the entire first surface 211.

[0098] The first elastic film layer 220 has elasticity to allow elastic deformation. For example, when a force is applied to the first elastic film layer 220 toward the bottom of the liquid storage chamber 212 using, for example, a push rod, the first elastic film layer 220 can be elastically deformed to allow the push rod to have a certain stroke, so that the channel 214 is split and the central area 213 forms a desired angle (such as 10 degrees to 25 degrees) with the side wall, and after the push rod is withdrawn to remove the force, the first elastic film layer 220 can be basically restored to the initial state without being damaged.

[0099] For example, the first elastic membrane layer 220 may be at least partially transparent, such as transparent or translucent, to allow observation of the liquid in the liquid storage chamber 212 and the liquid flow channel 215 or optical detection.

[0100] For example, the material of the first elastic film layer 220 can be polyethylene terephthalate (PET), polystyrene (PS), polymethyl methacrylate (PMMA), polypropylene (PP), polycarbonate (PC), etc., so as to have good elasticity and strength, so as to be able to restore to the original state after elastic deformation. Of course, the embodiments of the present disclosure are not limited to the above materials, and the first elastic film layer 220 can also be made of other applicable materials, such as a polymer composite material of PS and PET, so as to have better elasticity and strength.

[0101] Although in Figure 8 , the first elastic film layer 220 is shown as having a contour shape substantially the same as the first surface 211 of the substrate 210, however, it should be understood that the embodiments of the present disclosure are not limited thereto. For example, in some embodiments, the first elastic film layer 220 may only cover the bottom of the liquid storage chamber 212, and the groove of the liquid flow channel 215 is covered by other film layers (e.g., another elastic film layer or a rigid film layer), and the other film layer is sealed and connected to the first elastic film layer 220 to prevent liquid leakage, or the first elastic film layer 220 may cover the bottom of the liquid storage chamber 212 and the groove of the liquid flow channel 215, and the other parts of the first surface 212 are covered by other film layers or are no longer covered and directly exposed to the external environment.

[0102] In some embodiments, the detection chip 200 may further include a first adhesive layer 230 . The first adhesive layer 230 is between the substrate 210 and the first elastic film layer 220 to connect the substrate 210 and the first elastic film layer 220 .

[0103] For example, the first adhesive layer 230 may include an adhesive material such as an acrylic adhesive, and may be implemented as an adhesive coating or a double-sided adhesive. For example, the first adhesive layer 230 and the first elastic film layer 220 have substantially the same outer contour, so that the first adhesive layer 230 can achieve a firm combination of the substrate 210 and the first elastic film layer 220.

[0104] In some embodiments, when the first elastic film layer 220 covers the bottom of the liquid storage chamber 212 and the groove of the liquid flow channel 215, the first adhesive layer 230 may expose the groove of the liquid flow channel 215 and the bottom of the liquid storage chamber 212 on the first surface 211 of the substrate 210. That is, the first adhesive layer 230 may include a hollow area 231, the shape of which is the same or substantially the same as the orthographic projection of the liquid flow channel 215 and the liquid storage chamber 212 on the first adhesive layer 230, so as to facilitate the first elastic film layer 220 and the liquid flow channel 215 to form a space for, for example, liquid flow and / or reagent reaction. In other embodiments, when the first elastic film layer 220 only covers the bottom of the liquid storage chamber 212, the hollow area 231 of the first adhesive layer 230 may only expose the bottom of the liquid storage chamber 212.

[0105] In the case where the detection chip 200 includes the liquid operation area 219, the hollow area 231 of the first adhesive layer 230 may include an opening 2311 corresponding to the liquid operation area 219 to provide a space for liquid flow and / or reagent reaction between the substrate 210 and the first elastic film layer 220. For example, the liquid operation area 219 may be connected to the liquid flow channel 215. However, it should be understood that Fig. 9 The shape and position of the liquid operating area 219 shown in FIG. 2 are exemplary only and are not limited in the embodiments of the present disclosure.

[0106] In addition, if Figure 8 As shown, in some embodiments, the hollow area 231 of the first adhesive layer 230 may further include a liquid flow control area 2312. The projection of the liquid flow control area 2312 on the first surface 211 falls on the extension line of the liquid flow channel 215. In operation, when pressing is performed manually or by an action device at a position corresponding to the liquid flow control area 2312 on the first surface 211, the first elastic film layer 220 is elastically deformed so as to abut against the substrate 210, thereby blocking the flow of the liquid in the liquid flow channel 215, and when the pressure is no longer applied, the first elastic film layer 220 can basically return to the initial state without being damaged, and the liquid in the liquid flow channel 215 can also resume flowing. In this way, the flow of the liquid in the liquid flow channel 215 can be controlled according to actual requirements.

[0107] For example, in other embodiments, when the first elastic film layer 220 is bonded to the substrate 210 by heat pressing, ultrasonic welding, photosensitive adhesive bonding, chemical solvent bonding, or laser welding, the first adhesive layer 230 may be omitted. For example, when the first elastic film layer 220 and the substrate 210 are formed by the same polymer material, the first elastic film layer 220 and the substrate 210 may be bonded by laser welding without providing the first adhesive layer 230.

[0108] In addition, if Figure 8 As shown, the detection chip 200 according to at least one embodiment of the present disclosure may further include a second elastic film layer 240. Along the direction from the first surface 211 to the second surface 211', the second elastic film layer 240 is above the second surface 211' of the substrate 210 opposite to the first surface 211, and at least covers the opening 218 of the liquid storage chamber 212 on the second surface 211'. In some embodiments, the second elastic film layer 240 may also cover the entire second surface 211'. The second elastic film layer 240 may be liquid-tightly connected to the substrate 210 to prevent the liquid contained in the liquid storage chamber 212 from leaking from the opening 218 on the second surface 211'.

[0109] The second elastic film layer 240 has elasticity to allow elastic deformation. For example, when a force is applied to the second elastic film layer 240 toward the liquid storage chamber 212 (for example, by other action mechanisms or manually applying an action force), the second elastic film layer 240 can be elastically deformed to allow the liquid contained in the liquid storage chamber 212 to be squeezed (for example, so that the liquid contained in the liquid storage chamber 212 flows out from the bottom of the liquid storage chamber 212), thereby allowing the liquid to flow out from the opening formed at the bottom, and after the force is removed, the second elastic film layer 240 can be basically restored to the initial state, for example, the liquid contained in the liquid storage chamber 212 can be squeezed repeatedly multiple times to more fully allow the liquid to flow out from the opening formed at the bottom. For example, the second elastic film layer 240 can be at least partially transparent, such as transparent or translucent, to allow the liquid in the liquid storage chamber 212 to be observed or optically detected.

[0110] For example, the material of the second elastic film layer 240 can be polyethylene terephthalate (PET), polystyrene (PS), polymethyl methacrylate (PMMA), polypropylene (PP), polycarbonate (PC), etc., so as to have good elasticity and strength, so as to be able to restore to the original state after elastic deformation. Of course, the embodiments of the present disclosure are not limited thereto, and the second elastic film layer 240 can also be made of other applicable materials, such as a polymer composite material of PS and PET, so as to have better elasticity and strength.

[0111] The first elastic film layer 220 and the second elastic film layer 240 may be formed of the same or different materials, which is not limited in the embodiments of the present disclosure.

[0112] However, it should be understood that, in the case where the liquid storage chamber 212 does not have the opening 218 on the second surface 211 ′, the detection chip 200 may not include the second elastic membrane layer 240 .

[0113] Although in Figure 8 In the figure, the second elastic film layer 240 is shown to cover not only the opening 218 of the liquid storage chamber 212 on the second surface 211', but also other parts of the second surface 211' of the substrate 210. However, it should be understood that the embodiments of the present disclosure are not limited thereto. For example, in some embodiments, the second elastic film layer 240 may only cover the opening 218 of the liquid storage chamber 212 on the second surface 211'.

[0114] In some embodiments, the detection chip 200 may further include a second adhesive layer 250 . The second adhesive layer 250 is between the substrate 210 and the second elastic film layer 240 to connect the substrate 210 and the second elastic film layer 240 .

[0115] For example, the second adhesive layer 250 may include an adhesive material such as an acrylic adhesive, for example, and may be implemented as an adhesive coating or a double-sided adhesive. For example, the second adhesive layer 250 and the second elastic film layer 240 have substantially the same outer contour, so that the second adhesive layer 250 can achieve a firm combination of the substrate 210 and the second elastic film layer 240.

[0116] In some embodiments, the second adhesive layer 250 exposes the opening 218 of the liquid storage chamber 212 on the second surface 211 ′. That is, the second adhesive layer 250 may include a hollow area 251 having a shape that is the same or substantially the same as the orthographic projection of the opening 218 on the second adhesive layer 250 .

[0117] For example, in other embodiments, when the second elastic film layer 240 is bonded to the substrate 210 by heat pressing, ultrasonic welding, photosensitive adhesive bonding, chemical solvent bonding, or laser welding, the second adhesive layer 250 may be omitted. For example, when the second elastic film layer 240 and the substrate 210 are formed by the same polymer material, the second elastic film layer 240 and the substrate 210 may be bonded by laser welding without providing the second adhesive layer 250.

[0118] Fig. 12A and Fig. 12B According to at least one embodiment of the present disclosure Fig. 9 A cross-sectional view taken along line AA' in FIG. Fig. 12A and Fig. 12B Shows Fig. 9 Schematic diagram of the liquid release process of the detection chip shown. Fig. 12A and Fig. 12B As shown, when the push rod applies a force toward the bottom of the liquid storage chamber 212 (e.g., the central area 213), the channel 214 can be destroyed, the bottom of the liquid storage chamber 212 is split along the channel 214, the edge of the central area 213 surrounded by the channel 214 is separated from the other parts of the bottom of the liquid storage chamber 212, the connection portion 217 connects the central area 213 and the side wall 216, and the connection portion 217 allows the angle between the central area 213 and the side wall 216 to be greater than zero and less than 90 degrees, such as an angle of 10 degrees to 25 degrees. The liquid flow channel 215 is connected to the channel 214, and the liquid released from the liquid storage chamber 212 flows into the liquid flow channel 215 and is transported to other locations of the detection chip, such as the liquid operation area 219, via the liquid flow channel 215.

[0119] At least one embodiment of the present disclosure further provides a method for preparing a detection chip, which can be the detection chip provided by any of the above embodiments. For a detailed description of the detection chip, please refer to the description of each embodiment above, which will not be repeated herein.

[0120] For example, a method for preparing a detection chip according to at least one embodiment of the present disclosure may include:

[0121] providing a substrate, the substrate comprising a first surface,

[0122] The substrate includes a liquid storage chamber configured to contain liquid and included at the bottom of the first surface.

[0123] The bottom portion includes a central region and a peripheral region surrounding the central region, and

[0124] The edge region includes a channel surrounding the central region, and the thickness of the channel is less than the thickness of the central region.

[0125] In some embodiments, the method for preparing the detection chip may further include: preparing the substrate by an integrated molding method. For example, the integrated molding method includes an injection molding process, and the substrate can be prepared by the injection molding process and using a corresponding injection mold to save costs and improve production efficiency. In some embodiments, when preparing the substrate by the injection molding process, a liquid flow channel can also be formed in the substrate at the same time, and the embodiments of the present disclosure are not limited to this.

[0126] In some embodiments, the method for preparing the detection chip may further include: providing a first elastic film layer on the first surface of the substrate. The first elastic film layer is sealed and connected to the first surface of the substrate, and the first elastic film layer at least covers the bottom of the liquid storage chamber. For the description of the first elastic film layer, please refer to the description of each embodiment above, and will not be repeated herein.

[0127] In some embodiments, the method for preparing the detection chip may further include: bonding the first elastic film layer and the substrate by laser welding or adhesive. For example, when the first elastic film layer and the substrate are formed by the same material (such as polymer materials such as PS, PMMA, PC, PP, etc.), the first elastic film layer and the substrate may be bonded by laser welding; when the first elastic film layer and the substrate are formed by different materials, the first elastic film layer and the substrate may be bonded by, for example, adhesive.

[0128] In some embodiments, the step of bonding the first elastic film layer and the substrate by an adhesive may include: providing a first adhesive layer between the first elastic film layer and the substrate. The description of the first adhesive layer can refer to the description of each embodiment above, and will not be repeated herein.

[0129] In some embodiments, the method for preparing the detection chip may further include: providing a second elastic film layer on a second surface of the substrate opposite to the first surface. The first elastic film layer is sealed and connected to the second surface of the substrate. The second elastic film layer, for example, at least covers the opening of the liquid storage chamber on the second surface. For the description of the second elastic film layer, please refer to the description of each embodiment above, and will not be repeated herein.

[0130] In some embodiments, the method for preparing the detection chip may further include: bonding the second elastic film layer and the substrate by laser welding or adhesive. For example, when the second elastic film layer and the substrate are formed by the same material (such as polymer materials such as PS, PMMA, PC, PP, etc.), the second elastic film layer and the substrate may be bonded by laser welding; when the second elastic film layer and the substrate are formed by different materials, the second elastic film layer and the substrate may be bonded by, for example, adhesive.

[0131] In some embodiments, the step of bonding the second elastic film layer and the substrate by an adhesive may include: providing a second adhesive layer between the second elastic film layer and the substrate. The description of the second adhesive layer can refer to the description of each embodiment above, and will not be repeated herein.

[0132] At least one embodiment of the present disclosure further provides a method for operating a detection chip, wherein the detection chip may be the detection chip provided by any of the above embodiments. For a detailed description of the detection chip, please refer to the description of each embodiment above, which will not be repeated herein.

[0133] For example, a method for operating a detection chip according to at least one embodiment of the present disclosure may include:

[0134] A force is applied on the bottom of the liquid storage chamber toward the inner space of the liquid storage chamber to destroy the channel and cause the liquid contained in the inner space of the liquid storage chamber to flow out from the bottom of the liquid storage chamber.

[0135] In some embodiments, applying a force on the bottom of the liquid storage chamber toward the internal space of the liquid storage chamber to destroy the channel and make the liquid contained in the internal space of the liquid storage chamber flow out from the bottom of the liquid storage chamber, including: applying the force on the bottom of the detection chip toward the internal space of the liquid storage chamber so that the central area and the side wall form an angle greater than zero and less than 90 degrees. For example, the central area and the side wall form an angle of 0.1 degrees to 60 degrees, 5 degrees to 40 degrees, or 10 degrees to 25 degrees.

[0136] In some embodiments, when the detection chip further includes a first elastic film layer, the above-mentioned step of applying a force on the bottom of the liquid storage chamber toward the internal space of the liquid storage chamber may include: applying a force on the first elastic film layer to apply a force to the bottom of the liquid storage chamber, thereby destroying the channel and causing the liquid contained in the internal space of the liquid storage chamber to flow out from the bottom of the liquid storage chamber. The description of the first elastic film layer can refer to the description of each embodiment above, and will not be repeated herein.

[0137] In some embodiments, applying a force on the first elastic film layer to apply a force to the bottom of the liquid storage chamber, thereby destroying the channel and causing the liquid contained in the internal space of the liquid storage chamber to flow out from the bottom of the liquid storage chamber, may include: applying a force on the first elastic film layer at a position corresponding to a position 0-3 / 4 of the length of the central area of ​​the connecting portion, so as to apply a force to the bottom of the liquid storage chamber, thereby destroying the channel and causing the liquid contained in the internal space of the liquid storage chamber to flow out from the bottom of the liquid storage chamber. For example, a force may be applied to the first elastic film layer at a position corresponding to a position 1 / 2 of the length of the central area of ​​the connecting portion. In this way, when the first elastic film layer produces the same elastic deformation, the central area and the side wall form a smaller angle, which facilitates the liquid in the internal space of the liquid storage chamber to flow out from the bottom of the liquid storage chamber. The central area length mentioned here refers to the distance from the connecting portion to a portion of the side wall of the liquid storage chamber on the first surface, wherein the portion of the side wall and the connecting portion are located on opposite sides of the central area.

[0138] In some embodiments, when the detection chip further includes a second elastic film layer, the method for operating the detection chip may further include: applying a force on the second elastic film layer to drive the liquid contained in the internal space of the liquid storage chamber to flow out from the bottom of the liquid storage chamber. For the description of the second elastic film layer, please refer to the description of each embodiment above, which will not be repeated herein.

[0139] Fig.13 is a schematic block diagram of a detection device according to at least one embodiment of the present disclosure. Fig.13 As shown, the detection device 300 according to at least one embodiment of the present disclosure may include:

[0140] A detection chip 310; and

[0141] The force mechanism 320 is configured to apply a force on the bottom of the detection chip 310 toward the inner space of the liquid storage chamber when in use, so as to destroy the channel and make the liquid contained in the inner space flow out from the bottom of the detection chip 310.

[0142] The detection chip 310 can be a detection chip provided by any of the above embodiments. The force mechanism 320 can take any suitable form, as long as it can apply force to the bottom of the detection chip 310 to destroy the channel at the bottom of the liquid storage chamber in the detection chip 310. For example, the force mechanism 320 can include a rod to apply pressure to the bottom of the liquid storage chamber, thereby forming an opening at the bottom of the liquid storage chamber to allow the liquid stored therein to flow out, and the end of the rod can cooperate with the end surface of the bottom of the liquid storage chamber. The force mechanism 320 can be driven by a motor or can be manually operated, and the embodiments of the present disclosure are not limited to this.

[0143] In some embodiments, when the substrate of the detection chip 310 includes a connection portion, after the channel at the bottom of the liquid storage chamber is destroyed and the central area forms an angle greater than zero and less than 90 degrees (for example, an angle of 10 degrees to 25 degrees) with the side wall, the force mechanism 320 can be withdrawn or kept against the bottom of the detection chip 310 so that the central area and the side wall maintain the angle formed. For example, when the channel at the bottom of the liquid storage chamber is destroyed and the central area forms an angle greater than zero and less than 90 degrees (for example, an angle of 10 degrees to 25 degrees) with the side wall, the bending of the connection portion is plastic deformation, and the force mechanism 320 can be withdrawn. For example, when the channel at the bottom of the liquid storage chamber is destroyed and the central area forms an angle with the side wall that is greater than zero degrees and less than 90 degrees (for example, an angle of 10 degrees to 25 degrees), when the bending of the connecting portion is an elastic deformation, the force application mechanism 320 can remain against the bottom of the detection chip 310 so that the central area maintains the angle formed by the side wall, thereby preventing the central area from becoming horizontally placed and blocking the opening at the bottom of the liquid storage chamber as the liquid in the liquid storage chamber flows.

[0144] Although in Fig.13 Not shown, however, it should be understood that the detection device 300 may also include a base for placing the detection chip 310, a waste liquid processor, various analytical detectors, liquid input and output interfaces, a power interface, etc. These components can all be components known in the art, and the embodiments of the present disclosure are not limited to this.

[0145] The above description is merely an exemplary embodiment of the present disclosure and is not intended to limit the protection scope of the present disclosure. The protection scope of the present disclosure is determined by the appended claims.

Claims

1. A detection chip, comprising: a substrate including a first surface; wherein the substrate comprises a liquid storage chamber, the liquid storage chamber is configured to contain liquid and comprises a bottom located on the first surface, The bottom portion includes a central region and an edge region surrounding the central region, The edge region includes a channel surrounding the central region, the channel having a thickness less than a thickness of the central region; The detection chip further includes a first elastic film layer, which is on the first surface of the substrate and covers the bottom; The substrate also includes a liquid flow channel on the first surface, wherein the liquid flow channel is connected to the channel, and the detection chip also includes a first adhesive layer, the first adhesive layer is between the substrate and the first elastic film layer to connect the substrate and the first elastic film layer, the first adhesive layer includes a hollow area exposing the liquid flow channel and the bottom, the hollow area includes a liquid flow control area, and the projection of the liquid flow control area on the first surface falls on the extension line of the liquid flow channel.

2. The detection chip according to claim 1, wherein: The channel is a non-closed channel so as to partially surround the central area.

3. The detection chip according to claim 1, wherein: The channel is a closed channel so as to surround the central area.

4. The detection chip according to claim 1, wherein: The liquid storage chamber is cylindrical, and the channel is annular.

5. The detection chip according to claim 4, wherein: The central angle corresponding to the channel is 340 degrees to 358 degrees.

6. The detection chip according to claim 1, wherein: The thickness of the central area is 0.1 mm to 3 mm, and The thickness of the channel is 0.05 mm to 0.3 mm, and the width of the channel is 0.1 mm to 3 mm.

7. The detection chip according to any one of claims 1 to 6, wherein: The surface of the bottom facing the inner space of the liquid storage chamber is a plane, and the channel is recessed on the first surface toward the inner space of the liquid storage chamber.

8. The detection chip according to any one of claims 1 to 6, wherein: The surface of the bottom portion facing away from the inner space of the liquid storage chamber is a plane, and the channel is recessed away from the inner space of the liquid storage chamber at the surface of the bottom portion facing the inner space of the liquid storage chamber.

9. The detection chip according to claim 1 or 2, wherein: The liquid storage chamber further comprises a side wall, and the channel is between the side wall and the central area. The edge region also includes a connecting portion, The connecting portion is between the side wall and the central area, and corresponds to an opening portion of the channel that does not surround the central area.

10. The detection chip according to claim 9, wherein: The connection portion is configured to allow the central region to form an angle greater than zero and less than 25 degrees with the sidewall when the channel is broken.

11. The detection chip according to claim 10, wherein: On the first surface, the connection portion and the liquid flow channel are located on two opposite sides of the central area.

12. The detection chip according to claim 10, in, The liquid flow channel includes a groove on the first surface of the substrate, The first elastic film layer also covers the groove to provide a space for liquid flow.

13. The detection chip according to claim 12, further comprising a second elastic film layer, The substrate further includes a second surface opposite to the first surface, The second elastic film layer is on the second surface of the substrate and covers the opening of the liquid storage chamber on the second surface.

14. The detection chip according to claim 13, further comprising: The second adhesive layer, in, The second adhesive layer is between the substrate and the second elastic film layer to connect the substrate and the second elastic film layer.

15. The detection chip according to claim 10, further comprising a liquid operation area, The liquid operation area is in communication with the liquid flow channel, in, The liquid operation area includes at least one selected from the group consisting of a liquid mixing area, a liquid detection area, and a liquid storage area.

16. A detection device comprising: The detection chip according to any one of claims 1 to 15; as well as The force mechanism is configured to apply a force on the bottom of the detection chip toward the internal space of the liquid storage chamber when in use, so as to destroy the channel and make the liquid contained in the internal space flow out from the bottom.

17. A method for preparing a detection chip, comprising: providing a substrate, the substrate comprising a first surface, The substrate includes a liquid storage chamber, the liquid storage chamber is configured to contain liquid and is included at the bottom of the first surface, The bottom portion includes a central region and an edge region surrounding the central region, and The edge region includes a channel surrounding the central region, the channel having a thickness less than a thickness of the central region; The method further comprises providing a first elastic film layer, wherein the first elastic film layer is disposed on the first surface of the substrate and covers the bottom; The substrate further comprises a liquid flow channel on the first surface, wherein the liquid flow channel is connected to the channel, The method also includes providing a first adhesive layer, wherein the first adhesive layer is between the substrate and the first elastic film layer to connect the substrate and the first elastic film layer, the first adhesive layer includes a hollow area exposing the liquid flow channel and the bottom, the hollow area includes a liquid flow control area, and the projection of the liquid flow control area on the first surface falls on the extension line of the liquid flow channel.

18. The method of claim 17, further comprising: The substrate is prepared by an integrated molding method.

19. A method for operating the detection chip according to any one of claims 1 to 15, comprising: A force is applied on the bottom of the liquid storage chamber toward the inner space of the liquid storage chamber to destroy the channel and make the liquid contained in the inner space flow out from the bottom.

20. A method for operating the detection chip according to claim 12, comprising: A force is applied at a position on the first elastic membrane layer corresponding to a position 0-3 / 4 of the length of the central area of ​​the connecting portion to apply a force to the bottom of the liquid storage chamber, thereby destroying the channel and causing the liquid contained in the internal space of the liquid storage chamber to flow out from the bottom.

Images