Preparation method and application of tobacco powder extract

Abstract

The present invention provides a method for preparing a tobacco extract. The tobacco extract preparation method established by the present invention is low-cost and easy to implement. It not only solves many problems in the tobacco waste treatment process, but also effectively improves the value of tobacco waste, reduces cigarette processing costs, and improves the economic benefits of the enterprise; at the same time, the tobacco extract prepared by the invention effectively removes nicotine and enriches the aroma of the tobacco extract. The tobacco extract prepared by the present invention can effectively enrich the aroma of low-grade tobacco leaves, mask the impurities of low-grade tobacco leaves, improve the comfort of low-grade tobacco leaves, and greatly reduce the cost of low-grade tobacco leaf processing. The present invention uses a combination of natural fermentation and microbial fermentation to process tobacco, which can better extract the flavor substances of tobacco leaves, and at the same time prepare tobacco extracts with characteristic aromas according to demand, providing an effective way to process low-grade tobacco leaves.

Description

Technical Field

[0001] The invention belongs to the technical field of tobacco, and particularly relates to a preparation method and application of a tobacco dust extract. Background Art

[0002] The cigarette processing process generates a large amount of tobacco waste and low-grade tobacco leaves. At present, the tobacco industry has two main ways to deal with tobacco waste: one is to directly scrap it and treat it as industrial waste; the other is to sell it cheaply to tobacco leaf transformation production enterprises as raw materials for transformed tobacco leaves.

[0003] Some chemical components in tobacco are harmful to the environment. Disposing of tobacco waste directly as industrial waste not only negatively impacts the environment but also wastes raw materials and increases cigarette production costs. Selling it cheaply to tobacco leaf production companies, while generating revenue, doesn't fully realize its value. Therefore, developing a method that can effectively treat tobacco waste while increasing its value and boosting profits for companies is a pressing challenge for researchers in this field. Summary of the Invention

[0004] In view of this, the technical problem to be solved by the present invention is to provide a preparation method and application of tobacco extract. The method provided by the present invention can better extract the aromatic substances in tobacco, and at the same time can prepare tobacco extract with characteristic aroma according to demand, providing an effective way for the processing of low-grade tobacco leaves.

[0005] The present invention provides a method for preparing a tobacco extract, comprising the following steps:

[0006] A) mixing tobacco dust with an ethanol aqueous solution to obtain a tobacco dust suspension;

[0007] B) subjecting the tobacco dust suspension to natural fermentation to obtain a natural fermentation liquid;

[0008] C) subjecting the natural fermentation broth to reflux extraction to obtain an extract;

[0009] D) filtering the extract and then distilling under reduced pressure to obtain an extract;

[0010] E) adding nicotine-reducing straw bacteria and Hansenula sp. to the extract to carry out fermentation to obtain a fermentation liquid;

[0011] F) sterilizing the fermentation broth and concentrating it to obtain a paste extract.

[0012] Preferably, the volume fraction of the ethanol aqueous solution is 20% to 40%;

[0013] The material-liquid ratio of the tobacco powder to the ethanol aqueous solution is 1:15 to 1:20.

[0014] Preferably, the temperature of the natural fermentation is room temperature and the time is 24 to 48 hours.

[0015] Preferably, the temperature of the reflux extraction is 80-100° C., and the time is 2-3 hours.

[0016] Preferably, the nicotine-reducing straw bacteria and Hansenula sp. are added to the extract in the form of activated nicotine-reducing straw bacteria liquid and activated Hansenula sp. liquid, and the amount of the activated nicotine-reducing straw bacteria liquid added accounts for 4% to 6% of the mass of the extract, and the amount of the activated Hansenula sp. liquid added accounts for 4% to 6% of the mass of the extract.

[0017] Preferably, in step E), the fermentation temperature is 26-34° C. and the fermentation time is 18-24 hours.

[0018] The present invention also provides a tobacco extract prepared by the above preparation method.

[0019] The present invention also provides an application of the tobacco dust extract in low-grade tobacco strips.

[0020] The present invention also provides a method for preparing modified tobacco leaves, comprising the following steps:

[0021] After the low-grade tobacco strips are moistened, the above-mentioned tobacco dust extract is added and the leaves are stored to obtain low-grade tobacco strips after storage;

[0022] The low-grade tobacco leaves after storage are retested to obtain modified tobacco leaves.

[0023] Preferably, the moisture content of the low-grade tobacco sheet after moisture conditioning is 16% to 18%;

[0024] The tobacco dust extract accounts for 1.5% to 2% of the mass of the low-grade tobacco leaves;

[0025] The leaf storage time is 0.5 to 2 hours;

[0026] The temperature of the re-baking is 80-100°C;

[0027] The moisture content of the modified tobacco leaves is 11% to 13%.

[0028] Compared with the prior art, the present invention provides a preparation method of tobacco dust extract, comprising the following steps: A) mixing tobacco dust with an ethanol aqueous solution to obtain a tobacco dust suspension; B) subjecting the tobacco dust suspension to natural fermentation to obtain a natural fermentation liquid; C) subjecting the natural fermentation liquid to reflux extraction to obtain an extract; D) filtering the extract and then performing reduced pressure distillation to obtain an leaching solution; E) adding nicotine-reducing straw bacteria and Hansenula sp. to the leaching solution for fermentation to obtain a fermentation liquid; and F) sterilizing the fermentation liquid and concentrating it to obtain a paste extract.

[0029] The method for preparing the tobacco extract established by the present invention is low-cost and easy to implement. It not only solves many problems in the process of treating tobacco waste, but also effectively improves the value of tobacco waste, reduces cigarette processing costs, and improves the economic benefits of enterprises. At the same time, the tobacco extract prepared by the present invention effectively removes nicotine and enriches the aroma of the tobacco extract. The tobacco extract prepared by the present invention can effectively enrich the aroma of low-grade tobacco leaves, mask the impurities of low-grade tobacco leaves, improve the comfort of low-grade tobacco leaves, and greatly reduce the cost of treating low-grade tobacco leaves. The present invention uses a combination of natural fermentation and microbial fermentation to treat tobacco, which can better extract the aromatic substances of tobacco leaves, and at the same time prepare tobacco extracts with characteristic aromas according to needs, providing an effective way to treat low-grade tobacco leaves. DETAILED DESCRIPTION

[0030] The present invention provides a method for preparing a tobacco extract, comprising the following steps:

[0031] A) mixing tobacco dust with an ethanol aqueous solution to obtain a tobacco dust suspension;

[0032] B) subjecting the tobacco dust suspension to natural fermentation to obtain a natural fermentation liquid;

[0033] C) subjecting the natural fermentation broth to reflux extraction to obtain an extract;

[0034] D) filtering the extract and then distilling under reduced pressure to obtain an extract;

[0035] E) adding nicotine-reducing straw bacteria and Hansenula sp. to the extract to carry out fermentation to obtain a fermentation liquid;

[0036] F) sterilizing the fermentation broth and concentrating it to obtain a paste extract.

[0037] The method firstly mixes tobacco dust with an ethanol aqueous solution to obtain a tobacco dust suspension.

[0038] The present invention has no particular limitation on the source of the tobacco dust, and any tobacco dust known to those skilled in the art can be used, preferably tobacco dust waste and low-grade tobacco leaves generated during cigarette processing.

[0039] The volume fraction of the ethanol aqueous solution is 20% to 40%, preferably 20%, 25%, 30%, 35%, 40%, or any value between 20% and 40%;

[0040] The material-liquid ratio of the tobacco powder to the ethanol aqueous solution is 1:15 to 1:20, preferably 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, or any value between 1:15 and 1:20.

[0041] After obtaining the tobacco dust suspension, the tobacco dust suspension is subjected to natural fermentation to obtain a natural fermentation liquid.

[0042] The temperature of the natural fermentation is room temperature, which is a room temperature known to those skilled in the art. The time of the natural fermentation is 24 to 48 hours, preferably 24, 36, 48, or any value between 24 and 48 hours.

[0043] The natural fermentation broth is subjected to reflux extraction to obtain an extract, wherein the reflux extraction temperature is 80-100° C., preferably 80, 85, 90, 95, 100, or any value between 80-100° C., and the extraction time is 2-3 hours, preferably 2, 2.5, 3, or any value between 2-3 hours.

[0044] The extract is filtered and then subjected to vacuum distillation to obtain an extract. The present invention does not particularly limit the methods of filtering the extract and vacuum distillation, and any method known to those skilled in the art can be used. The vacuum distillation temperature is 45 to 60°C, preferably 45, 50, 55, 60, or any value between 45 and 60°C, and the pressure distillation is continued until the alcohol is evaporated.

[0045] Next, adding nicotine-reducing straw bacteria and Hansenula sp. into the extract to carry out fermentation to obtain a fermentation liquid.

[0046] The nicotine-reducing straw bacteria and Hansenula sp. are added to the extract in the form of activated nicotine-reducing straw bacteria liquid and activated Hansenula sp. liquid. The amount of the activated nicotine-reducing straw bacteria liquid added accounts for 4% to 6% of the mass of the extract, preferably 4%, 4.5%, 5%, 5.5%, 6%, or any value between 4% and 6%. The amount of the activated Hansenula sp. liquid added accounts for 4% to 6% of the mass of the extract, preferably 4%, 4.5%, 5%, 5.5%, 6%, or any value between 4% and 6%.

[0047] In the present invention, the activated nicotine-reducing straw bacterial solution is prepared according to the following method:

[0048] The frozen bacterial strain was inoculated into the solid culture medium and cultured in a constant temperature incubator at 30°C for 72 hours.

[0049] The activated bacteria were inoculated into the fermentation medium at a rate of 2 rings / 100 mL and cultured at 30°C and 200 rpm for 12 h.

[0050] The activated Hansenula yeast liquid is prepared according to the following method:

[0051] The frozen bacterial strain was inoculated into the solid culture medium and cultured in a constant temperature incubator at 28°C for 60 hours.

[0052] The activated bacteria were inoculated into the fermentation medium at a rate of 3 rings / 100 mL and cultured at 28°C and 220 r / min for 24 h.

[0053] The fermentation temperature is 26-34°C, preferably 26, 28, 30, 32, 34, or any value between 26-34°C, and the fermentation time is 18-24 hours, preferably 18, 20, 22, 24, or any value between 18-24 hours.

[0054] Finally, the fermentation liquid is sterilized and concentrated to obtain a paste extract.

[0055] The concentration is carried out by vacuum distillation, and the solid content in the paste extract accounts for 70%-75% of the total amount.

[0056] The present invention also provides a tobacco extract prepared by the above preparation method.

[0057] The present invention also provides an application of the tobacco dust extract in low-grade tobacco strips.

[0058] The present invention also provides a method for preparing modified tobacco leaves, comprising the following steps:

[0059] After the low-grade tobacco strips are moistened, the above-mentioned tobacco dust extract is added and the leaves are stored to obtain low-grade tobacco strips after storage;

[0060] The low-grade tobacco leaves after storage are retested to obtain modified tobacco leaves.

[0061] Among them, the low-grade tobacco refers to tobacco with low sensory quality, specifically tobacco that is difficult to use in the recipe.

[0062] The moisture content of the low-grade tobacco sheets after moisture conditioning is 16% to 18%, preferably 16%, 17%, 18%, or any value between 16% and 18%;

[0063] The tobacco extract accounts for 1.5% to 2% of the mass of the low-grade tobacco strips, preferably 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%, or any value between 1.5% and 2%;

[0064] The leaf storage time is 0.5 to 2 hours, preferably 0.5, 1, 1.5, 2, or any value between 0.5 and 2 hours;

[0065] The re-baking temperature is 80-100°C, preferably 80, 85, 90, 95, 100, or any value between 80-100°C;

[0066] The moisture content of the modified tobacco leaves is 11% to 13%, preferably 11%, 11.5%, 12%, 12.5%, or 13%.

[0067] The method for preparing the tobacco extract established by the present invention is low-cost and easy to implement. It not only solves many problems in the process of treating tobacco waste, but also effectively improves the value of tobacco waste, reduces cigarette processing costs, and improves the economic benefits of enterprises. At the same time, the tobacco extract prepared by the present invention effectively removes nicotine and enriches the aroma of the tobacco extract. The tobacco extract prepared by the present invention can effectively enrich the aroma of low-grade tobacco leaves, mask the impurities of low-grade tobacco leaves, improve the comfort of low-grade tobacco leaves, and greatly reduce the cost of treating low-grade tobacco leaves. The present invention uses a combination of natural fermentation and microbial fermentation to treat tobacco, which can better extract the aromatic substances of tobacco leaves, and at the same time prepare tobacco extracts with characteristic aromas according to needs, providing an effective way to treat low-grade tobacco leaves.

[0068] In order to further understand the present invention, the preparation method and application of the tobacco extract provided by the present invention are described below in conjunction with examples. The protection scope of the present invention is not limited by the following examples.

[0069] The tobacco dust used in the following examples is derived from tobacco dust produced in the cigarette processing process.

[0070] Low-grade tobacco leaves are "Three North Tobacco Leaves" with low formula applicability.

[0071] The activated nicotine-reducing straw bacterial solution was prepared as follows: the activated bacteria were inoculated into the fermentation culture solution at an inoculation rate of 2 rings / 100 ml, and cultured at 30° C. and 200 r / min for 12 hours.

[0072] The activated Hansenula yeast liquid was prepared as follows: the activated strain was inoculated into the fermentation medium at an inoculum rate of 3 rings / 100 ml, and cultured at 28° C. and 220 rpm for 24 hours.

[0073] Example 1

[0074] Tobacco dust is mixed with an ethanol aqueous solution to prepare a tobacco foam suspension, and then the tobacco foam suspension is naturally fermented to obtain a natural fermentation liquid.

[0075] The extract obtained after reflux extraction of the natural fermentation liquid is filtered and distilled under reduced pressure to obtain an extract.

[0076] adding nicotine-reducing straw bacteria and Hansenula sp. to the extract for fermentation to obtain a fermentation liquid;

[0077] The fermentation liquid is sterilized instantaneously at high temperature and then concentrated under reduced pressure to obtain a paste extract, namely, tobacco powder extract.

[0078] Table 1 Process parameters of tobacco extract

[0079]

[0080] On the basis of Example 1, only the fermentation time was changed to 18 hours and 28 hours respectively. Then, the aroma components and salinity of the obtained tobacco extract were determined. The results are shown in Table 2.

[0081] Table 2 Chemical composition comparison table

[0082] name comparison Fermentation 18 hours Fermentation for 24 hours Fermentation 28h Aroma components (μg / g) 206.43 251.26 367.31 378.94 Nicotine (mg / g) 4.68 2.54 0.76 0.57

[0083] In Table 2, "control" refers to the extract that was not fermented.

[0084] "Fragrance ingredients" include esters, carbonyls, acids, hydrocarbons, alcohols, and heterocyclic fragrance substances.

[0085] As can be seen from the above table, the aroma components reach the highest level and the nicotine content is the lowest when the fermentation time reaches 28 hours, but the change trend is not obvious compared to 24 hours. Considering the industrial value, 24 hours of fermentation is selected.

[0086] Next, after the low-grade tobacco leaves are moistened, the above-mentioned tobacco dust extract is added to the leaves for storage to obtain low-grade tobacco leaves after storage;

[0087] The low-grade tobacco leaves after storage were retested to obtain modified tobacco leaves. Specific process parameters are shown in Table 3, which lists the process parameters for the modified tobacco leaves.

[0088] Table 3 Process parameters for tobacco leaf transformation

[0089]

[0090] The tobacco leaves prepared in Examples 4 to 7 were subjected to sensory evaluation. The results are shown in Table 4.

[0091] Table 4 Standard sensory characteristics evaluation table (nine-point system)

[0092] sample Fragrance Aroma volume Miscellaneous gases concentration Momentum Irritation Aftertaste Comparison sample 3.0 3.0 3.0 4.5 4.5 3.5 3.5 0.5% addition 3.0 3.0 3.0 4.5 4.5 3.5 3.5 1.0% addition 3.5 3.5 3.5 5.0 4.5 3.5 3.5 1.5% addition 4.0 4.0 4.0 5.0 4.5 3.5 3.5 2.0% addition 4.5 4.0 4.0 5.5 4.5 3.0 3.0

[0093] In Table 4, the "control sample" is tobacco leaves processed under the process conditions of Example 4, but without adding fermentation liquid.

[0094] It can be seen from the above table that as the addition ratio increases, the aroma quality, aroma volume and smoke concentration increase, and the impurities decrease. When the addition ratio reaches 1.5%, the smoke is relatively balanced and the overall performance is the best; when the addition ratio reaches 2.0%, there is residue on the tongue, which affects the taste.

[0095] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A method for preparing a tobacco extract, characterized in that: The following steps are involved: A) mixing tobacco dust with an ethanol-water solution to obtain a tobacco dust suspension, wherein the volume fraction of the ethanol-water solution is 20% to 40%; The solid-liquid ratio of the tobacco powder to the ethanol aqueous solution is 1:15 to 1:20; B) subjecting the tobacco suspension to natural fermentation to obtain a natural fermentation liquid, wherein the natural fermentation temperature is room temperature and the time is 24 to 48 hours; C) subjecting the natural fermentation broth to reflux extraction to obtain an extract, wherein the reflux extraction is performed at a temperature of 80-100° C. for 2-3 hours; D) filtering the extract and then distilling under reduced pressure to obtain an extract; E) adding nicotine-reducing straw bacteria and Hansenula to the extract for fermentation to obtain a fermentation liquid, wherein the nicotine-reducing straw bacteria and Hansenula are added to the extract in the form of an activated nicotine-reducing straw bacteria liquid and an activated Hansenula liquid, wherein the activated nicotine-reducing straw bacteria liquid accounts for 4% to 6% of the mass of the extract, and the activated Hansenula liquid accounts for 4% to 6% of the mass of the extract, and the fermentation temperature is 26° C. to 34° C., and the fermentation time is 18 to 24 hours; F) sterilizing the fermentation broth and concentrating it to obtain a paste extract.

2. A tobacco extract prepared by the preparation method according to claim 1.

3. Use of the tobacco extract according to claim 2 in low-grade tobacco flakes.

4. A method for preparing modified tobacco leaves, characterized in that: The following steps are involved: After rehumidifying the low-grade tobacco strips, adding the tobacco dust extract according to claim 2 and storing the leaves to obtain low-grade tobacco strips after storage; The low-grade tobacco leaves after storage are redried to obtain modified tobacco leaves.

5. The preparation method according to claim 4, characterized in that The moisture content of low-grade tobacco leaves after rehumidification is 16%~18%; The tobacco dust extract accounts for 1.5% to 2% of the mass of the low-grade tobacco leaves; The leaf storage time is 0.5 to 2 hours; The temperature of the re-baking is 80-100°C; The moisture content of the modified tobacco leaves is 11% to 13%.

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