DNA fragmentation method and apparatus based on bubble interface vibration
By utilizing the vibration of the bubble interface within the flow channel to generate a tensile flow field and combining it with ultrasonic enhancement, the problems of contamination and non-random breakage in DNA fragmentation are solved, achieving efficient and contamination-free DNA molecular chain fragmentation.
CN115747302BActive Publication Date: 2026-06-30CHANGZHOU UNIV
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Patent Information
- Application Number
- CN202211488351.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-11-25
- Publication Date
- 2026-06-30
- Estimated Expiration
- 2042-11-25
AI Technical Summary
Technical Problem
Existing DNA fragmentation technologies suffer from problems such as cross-contamination of samples, large reagent requirements, and non-random breakpoints, leading to incorrect sequencing results.
Method used
A method based on bubble interface vibration is used to generate a stretching flow field by creating gradually contracting and expanding sections in the flow channel through bubbles, and the flow field is enhanced by ultrasound to achieve effective fragmentation of DNA molecular chains.
Benefits of technology
It achieves pollution-free DNA molecular chain fragmentation and can control tensile strength, improving fragmentation efficiency and accuracy and simplifying the subsequent cleaning process.
✦ Generated by Eureka AI based on patent content.
Abstract
This invention relates to a DNA fragmentation method and apparatus based on bubble interfacial vibration. The method includes: during the flow of a DNA sample along a flow channel, two bubbles from a bubble pair act symmetrically on both sides of the flow channel, creating a gradually contracting and expanding section within the channel to generate a stretching flow field; the DNA molecular chains in the DNA sample break due to the stretching effect of the stretching flow field as they pass through the contracting and expanding section. This invention can effectively fragment DNA molecules without contaminating them.
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