Yolk extraction device and method for preparing yolk antibodies

Abstract

The application relates to a yolk extraction device and a preparation method of egg yolk antibody. The extraction device comprises a workbench, a first linear module arranged on the workbench, a placing hole arranged on the first linear module, a pressing module arranged on the workbench and located above the first linear module, a lifting platform arranged on the workbench and located directly below the pressing module, an extraction shaft fixed at a first end of the lifting platform, a drill arranged on a second end of the extraction shaft and rotationally connected with the extraction shaft, a driving motor group arranged on the lifting platform, and two suction modules arranged on the lifting platform. The yolk extraction device and the preparation method of egg yolk antibody can directly extract egg white and egg yolk in the egg shell, so as to reduce the risk of contamination of the egg yolk liquid.

Description

Technical Field

[0001] This application relates to the field of biotechnology, and in particular to an egg yolk extraction device and a method for preparing egg yolk antibodies. Background Technology

[0002] Egg yolk antibodies are antibodies extracted from the yolks of eggs produced by immunizing laying hens. These antibodies can be used for the prevention and treatment of corresponding diseases. The separation process requires first cracking the eggshell, removing the egg white and yolk, and then separating the two.

[0003] The current separation method is mainly gravity separation. This method requires the egg white and yolk to move along a predetermined trajectory, and gravity will help the egg white and yolk separate. During this process, the egg white and yolk will be exposed to the air and move or flow along the equipment. There is a potential risk of contamination in this process, and bacteria can also grow on the egg liquid remaining on the equipment. Summary of the Invention

[0004] This application provides an egg yolk extraction device and a method for preparing egg yolk antibodies, which can directly extract egg white and egg yolk separately from inside the eggshell to reduce the risk of egg yolk contamination.

[0005] The above-mentioned objective of this application is achieved through the following technical solution:

[0006] In a first aspect, this application provides an egg yolk extraction device, comprising:

[0007] Workbench;

[0008] The first linear module is located on the worktable and configured to reciprocate linearly on the worktable.

[0009] The holes are placed evenly on the first linear module in an MxN matrix form, where M and N are both natural numbers greater than zero.

[0010] The clamping module is located on the worktable and above the first linear module;

[0011] The lifting platform is located on the workbench and directly below the clamping module.

[0012] The extraction shaft has its first end fixed to the lifting platform;

[0013] The drill bit is mounted on the second end of the extraction shaft and is rotatably connected to the extraction shaft.

[0014] The drive motor assembly is located on the lifting platform, and the transmission part of the driver connects to the drill bit after passing through a channel in the drill bit; and

[0015] Two suction modules are mounted on a lifting platform, and the flexible pipes of the suction modules can extend from the suction channel on the extraction shaft.

[0016] In one possible implementation of the first aspect, the clamping module includes:

[0017] The first telescopic actuator is located on the workbench;

[0018] A pressure plate is provided on the working end of the first telescopic actuator; and

[0019] The airbag is located on the working surface of the pressure plate.

[0020] In one possible implementation of the first aspect, positioning holes are evenly distributed on the working surface of the airbag.

[0021] In one possible implementation of the first aspect, a pressure sensor is provided inside the airbag, which is used to feed back a stop signal to the first telescopic actuator.

[0022] In one possible implementation of the first aspect, the maximum diameter of the drill bit is equal to the diameter of the extraction shaft.

[0023] In one possible implementation of the first aspect, the drive motor assembly includes:

[0024] The drive motor is located on the lifting platform; and

[0025] The drive shaft has one end connected to the output end of the drive motor, and the other end passes through the channel on the drill bit and is connected to the drill bit.

[0026] In one possible implementation of the first aspect, the absorbing module includes:

[0027] The second linear module is located on the lifting platform;

[0028] The suction pump is located on the second linear module; and

[0029] The flexible suction tube has one end connected to the input end of the suction pump, and the other end extending into the suction channel on the extraction shaft.

[0030] In one possible implementation of the first aspect, the extraction channel on the extraction axis includes a guide portion and a guide portion;

[0031] The second end of the flexible suction tube can extend from the guide portion of the suction channel;

[0032] The axis of the guide portion of the flexible suction tube intersects with the axis of the extraction shaft.

[0033] Secondly, this application provides a method for preparing egg yolk antibodies, comprising:

[0034] Disinfect the eggs;

[0035] After drilling a hole in the bottom of the egg, first remove the egg white from inside the shell, and then remove the egg yolk from inside the shell.

[0036] Add pure water to the egg yolks and mix well to obtain a diluted egg yolk solution;

[0037] Add a reaction solution containing phosphotungstic acid and magnesium chloride to the diluted egg yolk solution to obtain a mixture;

[0038] After thoroughly stirring the mixture, let it stand; and

[0039] The mixture was centrifuged and the supernatant was collected. The supernatant was then filtered and sterilized to obtain the egg yolk antibody solution. Attached Figure Description

[0040] Figure 1 This is a schematic diagram of a workbench and a first linear module provided in this application. The arrows in the diagram indicate the direction of movement of the placement holes.

[0041] Figure 2 This is a schematic diagram of an egg yolk extraction device provided in this application.

[0042] Figure 3 This is a schematic diagram illustrating the working principle of a clamping module provided in this application.

[0043] Figure 4 This is a schematic diagram illustrating the working principle of a drill bit provided in this application.

[0044] Figure 5 This is a schematic diagram illustrating the process by which the egg white and yolk flow out of the eggshell, as provided in this application.

[0045] Figure 6 This is a schematic diagram of the internal structure of an extraction shaft and the drive motor assembly provided in this application.

[0046] In the diagram, 2 is the clamping module, 4 is the suction module, 11 is the worktable, 12 is the first linear module, 13 is the placement hole, 21 is the first telescopic actuator, 22 is the pressure plate, 23 is the airbag, 24 is the positioning hole, 25 is the pressure sensor, 31 is the lifting platform, 32 is the extraction shaft, 33 is the drill bit, 34 is the drive motor assembly, 41 is the second linear module, 42 is the suction pump, 43 is the flexible suction tube, 341 is the drive motor, and 342 is the transmission shaft. Detailed Implementation

[0047] The technical solutions in this application will be further described in detail below with reference to the accompanying drawings.

[0048] Please see Figure 1 and Figure 2This application discloses an egg yolk extraction device, which consists of a worktable 11, a first linear module 12, a placement hole 13, a pressing module 2, a lifting platform 31, an extraction shaft 32, a drill bit 33, a drive motor group 34, and a suction module 4. The first linear module 12 is installed on the worktable 11 and can perform linear reciprocating motion on the worktable 11.

[0049] For ease of description, the two positions of the first linear module 12 on the workbench 11 are referred to as the preparation position and the extraction position, respectively. At the preparation position, the robot or the operator places the egg on the first linear module 12. At the extraction position, after the drill bit 33 drills a hole in the bottom of the egg, the two suction modules 4 suck out the egg white and yolk from the eggshell, respectively.

[0050] The extracted egg yolks can be directly stored in a sterile storage tank. This means that the egg yolks will not come into contact with air during the entire extraction process, nor will they come into excessive contact with the equipment as in existing extraction methods.

[0051] In some possible implementations, the first linear module 12 consists of three parts: a ball screw module, a movable worktable, and a guide rail. The ball screw module is arranged parallel to the guide rail, the movable worktable is fixedly connected to the ball screw module, and slidably connected to the guide rail, such as... Figure 1 As shown.

[0052] Please see Figure 2 The movable worktable is evenly distributed with placement holes 13. Each placement hole 13 is a through hole, with its two ends connected to the top and bottom surfaces of the movable worktable, respectively. In addition, the placement holes 13 are evenly distributed on the first linear module 12 in an M x N matrix form, where M and N are both natural numbers greater than zero. By increasing the number of placement holes 13, multiple eggs can be processed simultaneously, which can effectively improve work efficiency.

[0053] In some possible implementations, a row or column of holes 13 share a single suction module 4.

[0054] The clamping module 2 is mounted on the worktable 11 and located above the first linear module 12. Its function is to apply pressure to the egg inside the placement hole 13, keeping the egg stationary within the hole. Because the egg tends to move away from the placement hole 13 when the drill bit 33 is used to drill a hole, the clamping module 2 is needed to temporarily fix the egg within the hole 13. Figure 3 As shown.

[0055] The clamping module 2 consists of a first telescopic actuator 21, a pressure plate 22, and an air bladder 23. The first telescopic actuator 21 is fixedly mounted on the worktable 11 and can move towards and away from the placement hole 13. The pressure plate 22 is mounted on the first telescopic actuator 21 and can move with the working end (piston) of the first telescopic actuator 21.

[0056] The airbag 23 is mounted (e.g., by adhesive) on the pressure plate 22 and faces the placement hole 13. The surface of the pressure plate 22 facing the placement hole 13 is the working surface of the pressure plate 22. After the airbag 23 comes into contact with the egg located in the placement hole 13, the airbag 23 will deform at the point of contact.

[0057] At this time, the air bladder 23 will apply a downward pressure to the egg in contact with it, fixing the egg in place within the placement hole 13. At the same time, the deformation of the air bladder 23 can also play a certain positioning role to prevent the egg from tilting during subsequent drilling.

[0058] Please see Figure 2 Furthermore, positioning holes 24 are added to the working surface of the airbag 23. The positioning holes 24 are evenly distributed on the working surface of the airbag 23 and correspond one-to-one with the positions of the placement holes 13. The positioning holes 24 are equivalent to the active deformation of the airbag 23. The positioning holes 24 can increase the contact area between the airbag 23 and the egg, which can further improve the positioning effect mentioned above. In addition, the increase in contact area can also play a role in dispersing pressure.

[0059] Please see Figure 2 Furthermore, a pressure sensor 25 is installed inside the airbag 23. The pressure sensor 25 is used to send a stop signal back to the first telescopic actuator 21. The pressure sensor 25 has a battery and an antenna and can operate independently inside the airbag 23. The battery provides power, and the antenna is used to emit signals.

[0060] In a specific scenario, after airbag 23 comes into contact with the egg, the internal space decreases, causing the pressure inside airbag 23 to rise. There are two control methods at this point:

[0061] The first type is where the working stroke of the first telescopic actuator 21 is fixed, and the extension length of the piston is fixed each time;

[0062] The second type is where the working stroke of the first telescopic actuator 21 is not fixed, and the extension length is dynamically adjusted based on feedback from the pressure sensor 25.

[0063] Clearly, the second control method is more flexible because the eggs are of different sizes, and the dynamic adjustment of the extension length can keep the contact pressure between the airbag 23 and the egg within a stable range.

[0064] The lifting platform 31 is installed on the worktable 11 and located directly below the pressing module 2. The lifting platform 31 is equipped with an extraction shaft 32. Specifically, the first end of the extraction shaft 32 is fixed on the lifting platform 31, and the second end extends away from the lifting platform 31.

[0065] The drill bit 33 is mounted on the second end of the extraction shaft 32 and is rotatably connected to the extraction shaft 32. The power for the rotation of the drill bit 33 is provided by the drive motor assembly 34, which is mounted on the lifting platform 31. The transmission part of the drive motor assembly 34 passes through a channel on the drill bit 33 and is connected to the drill bit 33. That is, there is a hollow channel inside the extraction shaft 32, which supplies power to the transmission part of the drive motor assembly 34.

[0066] Two suction modules 4 are also installed on the lifting platform 31. The flexible pipes of both suction modules 4 can extend from the suction channels on the extraction shaft 32. As mentioned above, the extraction shaft 32 has a hollow channel and two suction channels. The hollow channel is used by the transmission part of the drive motor unit 34, and the two suction channels are used by the flexible pipes of the two suction modules 4 respectively.

[0067] After drill bit 33 drills into the bottom of the egg, the two suction channels will simultaneously connect with the space inside the egg. At this time, the flexible tube of the first suction module 4 extends out from one suction channel to extract the egg white liquid inside the egg. After the egg white liquid is extracted, the flexible tube of the second suction module 4 extends out from the other suction channel to extract the egg yolk liquid inside the egg.

[0068] It should be noted that drill bit 33 needs to move at a high speed and low feed rate to avoid the eggshell breaking.

[0069] To further describe a complete extraction process, firstly, the egg is placed in the placement hole 13 of the first linear module 12. Then, the first linear module 12 moves the egg directly below the pressing module 2. Next, the pressing module 2 actuates, applying downward pressure to the egg, pressing it against the inner wall of the placement hole 13. Figure 3 As shown.

[0070] At this time, the lifting platform 31 and the drive motor unit 34 start, and the drill bit 33 begins to rotate at high speed and move towards the bottom of the egg. After contacting the eggshell, the drill bit 33 will drill a hole at the bottom of the eggshell and enter the eggshell, such as... Figure 4 As shown.

[0071] After the drill bit 33 enters the eggshell, it stops rotating and seals the hole. The flexible tube of the first suction module 4 extends from the extraction shaft 32 and enters the space inside the eggshell to suck out the egg white liquid. Then, the flexible tube of the second suction module 4 extends from the extraction shaft 32 and enters the space inside the eggshell to suck out the egg yolk liquid.

[0072] Please see Figure 5 During the above process, there is a distance between the axis of drill 33 and the axis of the egg. This can be understood as drill 33 drilling into the eggshell from the center to the right (this is just an example, it could also be to the left). When the egg white is drawn, the yolk inside the eggshell will descend and come into contact with drill 33, and then slide down to the left along drill 33.

[0073] In this way, after the flexible tube of the second suction module 4 extends from the left side of the extraction shaft 32, it can suck the egg yolk liquid out of the eggshell.

[0074] As one specific embodiment of the egg yolk extraction device provided in the application, the maximum diameter of the drill bit 33 is equal to the diameter of the extraction shaft 32, so that when the drill bit 33 enters the eggshell, the extraction shaft 32 can seal the hole drilled by the drill bit 33 in the eggshell.

[0075] Please see Figure 6 As a specific embodiment of the egg yolk extraction device provided in the application, the drive motor assembly 34 consists of two parts: a drive motor 341 and a transmission shaft 342. The drive motor 341 is mounted on the lifting platform 31. The first end of the transmission shaft 342 is connected to the output end of the drive motor 341, and the second end passes through the channel on the drill bit 33 and is connected to the drill bit 33.

[0076] In some possible implementations, the drive shaft 342 and the drill bit 33 are connected by a threaded connection.

[0077] Please see Figure 1 As a specific embodiment of the egg yolk extraction device provided in the application, the suction module 4 is composed of a second linear module 41, a suction pump 42, and a flexible suction tube 43. The second linear module 41 is installed on the lifting platform 31, and its function is to drive the suction pump 42 installed thereon to move back and forth in the vertical direction.

[0078] The first end of the flexible suction tube 43 is connected to the input end of the suction pump 42, and the second end extends into the suction channel on the extraction shaft 32. When the second linear module 41 drives the suction pump 42 to reciprocate in the vertical direction, the flexible suction tube 43 will also extend out of or return to the suction channel on the extraction shaft 32.

[0079] Specifically, before the drill bit 33 enters the eggshell and during the drilling process, the flexible suction tube 43 needs to be located in the suction channel on the extraction shaft 32. After the drill bit 33 enters the eggshell, the flexible suction tube 43 needs to extend out from the suction channel on the extraction shaft 32.

[0080] The suction channel on the extraction shaft 32 includes a guide portion and a guide portion, and the second end of the flexible suction tube 43 can extend from the guide portion of the suction channel. The main function of the guide portion is to guide the orientation of the second end of the flexible suction tube 43 to change.

[0081] This requires that the axis of the guide portion of the flexible suction tube 43 intersects with the axis of the extraction shaft 32.

[0082] It should be understood that during the process of extracting egg white liquid, the first flexible suction tube 43 needs to be as close as possible to the inner wall of the eggshell so that the shape of the yolk will not be scattered during the fall of the egg white liquid.

[0083] During the extraction of egg yolk, the second flexible suction tube 43 does not need to be attached to the inner wall of the eggshell. After extending from the drill bit 33, it can be directly inserted into the space inside the eggshell. In this way, as the egg yolk flows out and falls with the egg white, it can directly contact the second flexible suction tube 43, and the second flexible suction tube 43 can even pierce directly into the egg yolk.

[0084] The guide section of the suction channel is used to guide the two flexible suction tubes 43 after they extend from the guide section on the drill bit 33, in order to meet the usage requirements in the above process.

[0085] This application also discloses a method for preparing egg yolk antibodies, comprising the following steps:

[0086] S101, sterilizes eggs;

[0087] S102, after drilling a hole in the bottom of the egg, first remove the egg white from inside the eggshell, and then remove the egg yolk from inside the eggshell;

[0088] S103, add pure water to the egg yolk and mix well to obtain a diluted egg yolk solution;

[0089] S104, add a reaction solution containing phosphotungstic acid and magnesium chloride to the egg yolk dilution solution to obtain a mixture;

[0090] S105, after thoroughly stirring the mixture, let it stand; and

[0091] S106, the mixture is centrifuged and the supernatant is collected. The supernatant is then filtered and sterilized to obtain egg yolk antibody solution.

[0092] Steps S101 and S102 have been described above and will not be repeated here.

[0093] In step S103, the egg yolks and pure water are thoroughly mixed in a ratio of 1:4-7.

[0094] In step S104, the concentration of phosphotungstic acid in the reaction solution is 0.3-0.5%, and the concentration of magnesium chloride is 0.6-0.9%. After phosphotungstic acid dissolves in water, it forms a free polyanion, which combines with Mg2+ in magnesium chloride, which is also soluble in water. This combination forms an insoluble complex with the lipoproteins in the diluted egg yolk solution. The liquid obtained after separating the insoluble complex is the solution containing egg yolk antibodies.

[0095] The embodiments described in this specific implementation are preferred embodiments of this application and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. An egg yolk extraction device, characterized in that, include: Worktable (11); First linear module (12), located on the worktable (11), configured to reciprocate linearly on the worktable (11); Place holes (13) evenly distributed on the first linear module (12) in the form of an MxN matrix, where M and N are both natural numbers greater than zero; The clamping module (2) is located on the worktable (11) and above the first linear module (12); A lifting platform (31) is located on the workbench (11) and directly below the clamping module (2); an extraction shaft (32) is fixed at its first end on the lifting platform (31); a drill bit (33) is located at the second end of the extraction shaft (32) and is rotatably connected to the extraction shaft (32); a drive motor assembly (34) is located on the lifting platform (31), and the transmission part of the drive motor assembly (34) passes through the channel on the extraction shaft (32) and is connected to the drill bit (33); Two suction modules (4) are mounted on the lifting platform (31). The flexible pipes of the suction modules (4) extend from the suction channels on the extraction shaft (32). There is a hollow channel and two suction channels on the extraction shaft (32). The hollow channel is used by the transmission part of the drive motor unit (34), and the two suction channels are respectively used by the flexible pipes of the two suction modules (4). The suction module (4) includes a second linear module (41) mounted on the lifting platform (31); a suction pump (42) mounted on the second linear module (41); and a flexible suction tube (43) with its first end connected to the input end of the suction pump (42) and its second end extending into the suction channel on the extraction shaft (32). The suction channel on the extraction shaft (32) includes a guide portion and a guide portion. The axis of the guide portion of the flexible suction tube (43) intersects the axis of the extraction shaft (32). The second ends of the flexible suction tubes (43) of the two suction modules (4) extend from the guide portions of the two suction channels on the extraction shaft (32), respectively.

2. The egg yolk extraction device according to claim 1, characterized in that, The clamping module (2) includes: The first telescopic actuator (21) is mounted on the worktable (11); A pressure plate (22) is provided on the working end of the first telescopic actuator (21); and An airbag (23) is placed on the working surface of the pressure plate (22).

3. The egg yolk extraction device according to claim 2, characterized in that, Positioning holes (24) are evenly distributed on the working surface of the airbag (23).

4. The egg yolk extraction device according to claim 2 or 3, characterized in that, The airbag (23) is equipped with a pressure sensor (25), which is used to send a stop signal to the first telescopic actuator (21).

5. The egg yolk extraction device according to claim 1, characterized in that, The maximum diameter of the drill bit (33) is equal to the diameter of the extraction shaft (32).

6. The egg yolk extraction apparatus according to claim 1 or 5, characterized in that, The drive motor assembly (34) includes: A drive motor (341) is mounted on the lifting platform (31); and The drive shaft (342) has its first end connected to the output end of the drive motor (341), and its second end passes through the channel on the extraction shaft (32) and is connected to the drill bit (33).

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