Nanosecond magnetic field irradiation device and design method and data acquisition method thereof

Abstract

The application relates to the technical field of biological effects of electromagnetic fields and its application, and discloses a nanosecond magnetic field irradiation device, a design method thereof and a data acquisition method. The design method of the nanosecond magnetic field irradiation device establishes a multi-frequency pulse electromagnetic environment under the condition that cells in the same column of holes in a 96-hole plate are irradiated by magnetic fields with the same intensity, the magnetic field output amplitude, the repetition frequency and the duration are continuously adjustable, the cells are continuously irradiated, the relationship between the cancer cell viability and PMF parameters such as the magnetic field rising rate dB / dt and the magnetic induction intensity B under the action of magnetic fields with different parameters is researched, and the magnetic field parameter action interval for effectively inhibiting the proliferation of cancer cells is obtained.

Description

Technical Field

[0001] This invention relates to the field of biological effects of electromagnetic fields and their applications, specifically to a nanosecond-level magnetic field irradiation device and its design and data acquisition methods. Background Technology

[0002] In the field of bioelectromagnetics, the biological effects and applications of electromagnetic fields are a very important topic. This is because the magnetic permeability μ of biological organisms differs from that in a vacuum. Similarly, magnetic fields penetrate living organisms more readily than electric fields, thus directly affecting internal organs, tissues, cells, and even cell nuclei. Pulsed magnetic fields are characterized by steep rise times, wide frequency distributions, and significant non-thermal effects, and pulsed electromagnetic field therapy has long been a hot research topic in cancer treatment. Therefore, the rational design of nanosecond-level pulsed current electromagnetic field irradiation devices has a significant impact on overcoming the challenges of cancer treatment.

[0003] Current research on pulsed electromagnetic field cancer therapy focuses primarily on experimental studies. The experimental methods and approaches mainly concentrate on analyzing the changes in various biological indicators caused by magnetic field irradiation, while there is insufficient analysis on the changes in the electromagnetic environment in the body caused by changes in magnetic field parameters, and a lack of analysis on the transient changes in the instantaneous biological effects of pulsed electromagnetic environment. Summary of the Invention

[0004] The problem this invention aims to solve is to provide a design method for a nanosecond-level irradiation device, addressing the shortcomings of current research on pulsed electromagnetic field cancer therapy. This device allows for continuous adjustment of the magnetic field amplitude, repetition frequency, and duration. The nanosecond-level irradiation device designed using this method is significant in overcoming the current research's lack of analysis on changes in the electromagnetic environment within organisms caused by variations in magnetic field parameters.

[0005] To achieve the above objectives, a first aspect of the present invention provides a design method for a nanosecond-level magnetic field irradiation device, the design method comprising:

[0006] S1. Select different magnetic field generating devices according to the pulse width requirements of different currents;

[0007] S2, irradiate the cells above the 96-well plate with a magnetic field so that the intensity of magnetic field irradiation on the cells cultured in each well of the 96-well plate is equal.

[0008] S3, build a nanosecond current source according to the requirements of nanosecond-level square waves.

[0009] The nanosecond-level magnetic field irradiation device designed using this method can achieve continuous adjustment of magnetic field amplitude, repetition frequency, and duration. This is of great significance in making up for the lack of analysis of changes in the electromagnetic environment in organisms caused by changes in magnetic field parameters in current research.

[0010] Furthermore, in S1:

[0011] A long straight conductor of length 2L is selected as the magnetic field generating device, carrying a current of I. The magnitude of the magnetic induction intensity at any point outside the long straight conductor is:

[0012] (1)

[0013] In equation (1), L is half the length of the long straight conductor, B is the magnetic induction intensity at any point outside the long straight conductor, and z is the relative distance between the projection of the midpoint of the long straight conductor and the measured point onto the long straight conductor. R is the permeability of vacuum, and R is the distance from the current source.

[0014] In S2: The long straight conductor is a coaxial cable, and a coaxial cable with the same length as the width of the 96-well plate is covered on the 96-well plate to make the magnetic field irradiation intensity of each well cell equal.

[0015] S3 includes: calculating the general solution of the wave equation of the lossless uniform transmission line based on the telegraph equation, and then obtaining the relationship between the nanosecond-level voltage wave and the current wave in the lossless uniform transmission line from the general solution:

[0016] (8)

[0017] In the formula: Z0 is the characteristic impedance of a lossless uniform transmission line.

[0018] A second aspect of the present invention provides a nanosecond-level magnetic field irradiation device obtained by the aforementioned nanosecond-level magnetic field irradiation device design method; the nanosecond-level magnetic field irradiation device further includes: a grounding copper plate and a nanosecond-level square wave generator disposed on the upper surface of the grounding copper plate; the 96-hole plate is disposed on the upper surface of the grounding copper plate;

[0019] The main circuit of the nanosecond-level square wave generator includes a PLC, a high-voltage source, a current-limiting resistor, a coaxial cable, a switch, and a matching impedance.

[0020] The coaxial cable covers the upper surface of the 96-hole plate and the length of the covering is equal to the width of the 96-hole plate.

[0021] The nanosecond-level magnetic field irradiation device of this invention improves upon existing irradiation devices by maintaining a stable magnetic field while ensuring the generation of the magnetic field. Compared to the original device, the improved irradiation device can establish a multi-band pulsed electromagnetic environment while ensuring that the cells in each well of a 96-well plate receive the same magnetic field irradiation intensity. This allows for continuous irradiation experiments on cells, enabling the study of the relationship between cell viability and PMF parameters such as the magnetic field rise rate (dB / dt) and magnetic induction intensity (B) under different magnetic field parameters. This facilitates the determination of the effective range of magnetic field parameters for inhibiting the proliferation of specific cells. The nanosecond-level irradiation device of this invention can be applied to various fields of electromagnetic effects on cells.

[0022] Furthermore, the 96-well plate is made of optically transparent pure polystyrene.

[0023] Furthermore, the switch is a mercury switch.

[0024] A third aspect of the present invention provides a method for acquiring cell response characteristic data, utilizing the aforementioned nanosecond-level magnetic field irradiation device, the method comprising:

[0025] Cells were cultured in 96-well plates with the cells adhering to the plate.

[0026] The coaxial cable is charged. After the charging and energy storage are completed, the output of the coaxial cable is used as the input of the coaxial switching device to form a square wave current, so that the magnetic field irradiation intensity received by the cells in each column of the 96-well plate is the same.

[0027] Using magnetic field amplitude, duration of magnetic field action, and frequency of magnetic field action as independent variables, response characteristic data of experimental cells in wells of a 96-well plate with the same magnetic field irradiation intensity were obtained under electromagnetic field action with different parameters.

[0028] The nanosecond-level magnetic field irradiation device obtained through data acquisition methods can achieve continuous adjustment of magnetic field amplitude, repetition frequency, and duration, which is of great significance in making up for the lack of analysis of changes in the electromagnetic environment in organisms caused by changes in magnetic field parameters in current research.

[0029] Furthermore, by changing the amplitude of the charging voltage, the amplitude of the output magnetic field is changed;

[0030] The duration of the magnetic field's effect can be adjusted by changing the length of the long straight conductor.

[0031] The frequency of the magnetic field is adjusted by changing the switching frequency of the discharge switch.

[0032] Other features and advantages of the present invention will be described in detail in the following detailed description section. Attached Figure Description

[0033] Figure 1 It is a schematic diagram showing the relationship between voltage and current in an incident or reflected wave;

[0034] Figure 2 This is a schematic diagram of the transmission line discharging through resistor R.

[0035] Figure 3 This is a schematic diagram of one embodiment of the nanosecond-level magnetic field irradiation device.

[0036] Figure 4 These are a structural schematic diagram of a 96-well plate and a front view of its specifications.

[0037] Figure 5 This is a schematic diagram of the simulation results in MATLAB. Detailed Implementation

[0038] The following provides a detailed description of specific embodiments of the present invention. It should be understood that the specific embodiments described herein are for illustrative and explanatory purposes only and are not intended to limit the scope of the invention.

[0039] In this invention, unless otherwise stated, directional terms such as "upper" and "lower" generally refer to the orientation in the assembled and used state. "Inner" and "outer" refer to the inner and outer sides relative to the outline of each component itself.

[0040] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this invention are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate for the embodiments of the invention described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0041] The first aspect of this invention provides a design method for a nanosecond-level magnetic field irradiation device. This method considers the requirements of the pulse generator structure, pulse rise time, capacitor energy storage capacity, and capacitor output duration to ensure optimal transmission performance for steep pulses. A long straight conductor is used as the magnetic field generator. The 96-well plate in the irradiation device is made of optically transparent pure polystyrene. A coaxial cable with a length equal to the width of the 96-well plate is covered on it. The distance between the two wells of the 96-well plate is calculated, considering the magnetic field irradiation intensity experienced by each well cell. The main components of the main circuit of the single-transmission-line nanosecond-level square wave generator include a PLC, a high-voltage source, a current-limiting resistor, a coaxial cable, a switch, and a matching impedance. The device designed according to this method meets the requirement of good transmission performance for steep pulses.

[0042] The design method for a nanosecond-level irradiation device first selects different magnetic field generators based on the pulse width requirements of different currents. Second, it determines the spacing between the two wells of a 96-well plate, ensuring that each well receives the same magnetic field irradiation intensity. Finally, it constructs a nanosecond current source according to the wave requirements. The specific design method is as follows:

[0043] A long straight conductor of length 2L is selected as the magnetic field generating device, carrying a current of I. The magnitude of the magnetic induction intensity at any point outside the long straight conductor is:

[0044] (1)

[0045] In equation (1), L is half the length of the long straight conductor, B is the magnetic induction intensity at any point outside the long straight conductor, and z is the relative distance between the projection of the midpoint of the long straight conductor and the measured point onto the long straight conductor. R is the permeability of vacuum, and R is the distance from the current source.

[0046] In one optional embodiment, the 96-well plate is made of optically transparent pure polystyrene. A coaxial cable 3 is used as the long straight conductor, covering the 96-well plate 2 with a length equal to the width of the plate, ensuring that the magnetic field irradiation intensity experienced by each well is equal. Solid polyethylene coaxial cable is used as the long straight conductor when constructing the circuit. The inner conductor of the coaxial cable is a copper core, and the outer conductor is a metal mesh conductive layer. The outer conductor is grounded to act as an electromagnetic shielding layer for the inner conductor, ensuring distortion-free transmission of electromagnetic waves within the coaxial cable and maintaining its normal electrical characteristics. Matching resistors are installed at both ends of the cable to reduce signal reflection.

[0047] When constructing a nanosecond current source, a transmission line composed of parallel ideal conductors is placed along the z-axis, according to the telegraph equation of a lossless uniform transmission line:

[0048] (2)

[0049] (3)

[0050] In the formula: U is the input voltage, I is the current, and z is the position of any point on the z-axis. L 0 represents the inductance per unit length of the transmission line. C 0 is the capacitance per unit length of the transmission line, expressed in F.

[0051] The wave equation for a lossless uniform transmission line is derived as follows:

[0052] (4)

[0053] (5)

[0054] Calculate its general solution:

[0055] (6)

[0056] (7)

[0057] In the formula: and These are incident voltage and current waves propagating in the (+z) direction, while and It is a reflected voltage wave and current wave propagating in the (-z) direction; t is time, z is the transmission distance, and v is the wave propagation speed.

[0058] The wave propagation speed satisfies Substituting the above equation into (6), we can obtain the relationship between voltage and current waves in a lossless uniform transmission line, i.e.

[0059] (8)

[0060] In the formula: Z 0 is the characteristic impedance of a lossless uniform transmission line.

[0061] in, It reflects the relationship between voltage and current in the incident or reflected wave. Voltage and current waves on transmission lines are generally the superposition of the incident and reflected waves, such as... Figure 1 As shown.

[0062] The working principle of the transmission line type square wave generator is as follows: First, a DC power supply is used to charge the transmission line, which has a length of l and a wave impedance of Z0. When time t=0, switch K is closed, and the transmission line discharges through resistor R. Figure 2 As shown.

[0063] Depend on Figure 1 We can consider the transmission line as two lines with the same voltage, and the wave propagation time is... τ= l / v Waves propagating in opposite directions. Let the two voltage waves be... U + and U - ,but U + = U - = U0 / 2.

[0064] According to the basic wave equation:

[0065] (9)

[0066] The reflection coefficient β and refractive index α at points A and B are calculated as follows, since the selected charging resistor value is relative to the load impedance. R L Since the reflection coefficient is infinite, point A can be considered to be in an open circuit state. Therefore, the reflection coefficient at point A is... and refractive index for:

[0067]

[0068] Reflectance at point B and refractive index for:

[0069] (10)

[0070] In the formula: It is the reflection coefficient. It is the refractive index. It is the load impedance. Z 0 is the characteristic impedance of a lossless uniform transmission line.

[0071] If the forward and reverse oscillations of the generated square wave pulse are required to be no greater than 10% of the peak value of the output current, the circuit parameters can be calculated according to formula (10) when the forward and reverse polarity oscillations are no greater than 10% of the peak value of the impact. The oscillation amplitude of the output pulse is mainly generated by the first reflection. As long as the amplitude of the first oscillation is no greater than 10% of the peak value of the impact, the condition for square wave oscillation is:

[0072] (11)

[0073] Therefore, we can conclude that:

[0074] (12)

[0075] That is, as long as the load impedance satisfies equation (12), a pulsed square wave can be generated using a uniform transmission line. After the switch is turned on, the amplitude of the square wave pulse obtained on the load is half of the charging voltage, and the pulse width is twice the propagation time of the electromagnetic wave in the transmission line. The main components of a single transmission line nanosecond-level square wave generator include a PLC, a high-voltage source, a current-limiting resistor, a coaxial cable, a switch, and a matching impedance.

[0076] Solid polyethylene coaxial cable was used as the transmission line when constructing the circuit. The inner conductor of the coaxial cable is a copper core, and the outer conductor is a metal mesh conductive layer. The outer conductor is grounded to act as an electromagnetic shielding layer for the inner conductor, ensuring undistorted transmission of electromagnetic waves within the coaxial cable and maintaining its normal electrical characteristics. Matching resistors are installed at both ends of the cable to reduce signal reflection. Its inductance per unit length is:

[0077] (13)

[0078] In the formula: μ 0 is the permeability of free space. μ r is the relative permeability, a is the inner diameter of the cable, and b is the outer diameter of the cable.

[0079] The capacitance per unit length is:

[0080] (14)

[0081] In the formula: ε 0 is the vacuum permittivity. ε r It is the relative permittivity.

[0082] Because the actual cable length used is relatively short, the line resistance and leakage conductance can be ignored, and it can be regarded as a lossless uniform transmission line. In this case, the characteristic impedance of the coaxial cable can be derived as follows:

[0083] (15)

[0084] In the formula: μ is the permeability and ε is the dielectric constant.

[0085] The beneficial effect of this invention is that, while ensuring the generation of a stable magnetic field by the magnetic field generator, it improves the existing irradiation device structure to a nanosecond-level irradiation device. Compared with the original device, the improved irradiation device can establish a multi-band pulsed electromagnetic environment while ensuring that the cells in each well of a 96-well plate receive the same magnetic field irradiation intensity. This allows for continuous irradiation experiments on cells, and the study of the relationship between cell viability and PMF parameters such as the magnetic field rise rate dB / dt and magnetic induction intensity B under different magnetic field parameters. This facilitates the determination of the effective range of magnetic field parameters for inhibiting cell proliferation. Of course, the nanosecond-level irradiation device of this invention can also be applied to various other fields of electromagnetic effects on cells.

[0086] A high-voltage DC output signal charges the coaxial cable via a coaxial switching device. After charging and energy storage, the output of the coaxial cable serves as the input to the coaxial switching device, passing through a discharge switch and matching resistors, and is output to ground, forming a square wave current. To ensure a steep rise time and high repetition rate for the nanosecond signal, a mercury switch is used as the discharge switch and installed in the coaxial device to prevent signal distortion. The amplitude of the output magnetic field can be changed by altering the charging voltage. The duration of the output magnetic field can be continuously adjusted by changing the transmission line length. The repetition frequency of the output magnetic field can be adjusted by changing the switching frequency of the mercury switch.

[0087] At the start of irradiation, cells were initially cultured at a fixed concentration in 96-well plates for 24 hours. After 24 hours, the 96-well plates were placed in an irradiation magnetic field. The 96-well plates were 10 cm wide, and a 10 cm long coaxial cable was placed over them. The distance between each adjacent well was 0.9 cm. During the experiment, the cells in each well adhered and grew at the bottom of the well; therefore, the distance between the coaxial cable (i.e., the current-carrying wire) and the cells can be considered as... Figure 4 As shown, 1cm.

[0088] Based on the dimensions of the 96-hole plate and the calculation formula (1) for the transmission line magnetic field, and taking the current magnitude as 5A, the simulation results in MATLAB are as follows. Figure 5 As shown in the diagram, the Y and Z coordinates represent the spatial coordinates of the magnetic field measurement point. The magnetic induction intensity decreases with increasing horizontal distance from the transmission line. When the horizontal distance is constant, the magnetic induction intensity is the same in the same column, so the magnetic field irradiation intensity received by the cells in each column of wells is the same in the experiment.

[0089] Based on the calculations and measurements above, it can be seen that cells in wells equidistant from the long straight conductor experience the same magnetic field irradiation intensity. Therefore, the magnetic field irradiation intensity is the same in any row of 6 wells in a 96-well plate. When nanosecond-level pulsed electromagnetic fields are applied, the magnetic field amplitude, duration, and frequency can be selected as independent variables to study the response characteristics of cancer cells under different electromagnetic field parameters. A horizontal comparison of the response curves under the same electromagnetic field can provide a reference for specific therapies.

[0090] A second aspect of the present invention provides a nanosecond-level magnetic field irradiation device obtained by the aforementioned nanosecond-level magnetic field irradiation device design method; the nanosecond-level magnetic field irradiation device further includes: a grounding copper plate 4 and a nanosecond-level square wave generator 1 disposed on the upper surface of the grounding copper plate 4. A 96-hole plate 2 is disposed on the upper surface of the grounding copper plate 4. The main circuit of the nanosecond-level square wave generator 1 includes a PLC, a high-voltage source, a current-limiting resistor, a coaxial cable 3, a switch, and a matching impedance. The coaxial cable 3 covers the upper surface of the 96-hole plate 2, and its coverage length is equal to the width of the 96-hole plate 2.

[0091] The nanosecond-level magnetic field irradiation device of this invention improves upon existing irradiation devices by maintaining a stable magnetic field while ensuring the generation of the magnetic field. Compared to the original device, the improved irradiation device can establish a multi-band pulsed electromagnetic environment while ensuring that the cells in each well of a 96-well plate receive the same magnetic field irradiation intensity. This allows for continuous irradiation experiments on cells, enabling the study of the relationship between cell viability and PMF parameters such as the magnetic field rise rate (dB / dt) and magnetic induction intensity (B) under different magnetic field parameters. This facilitates the determination of the effective range of magnetic field parameters for inhibiting the proliferation of specific cells. The nanosecond-level irradiation device of this invention can be applied to various fields of electromagnetic effects on cells.

[0092] A DC high-voltage output signal charges the coaxial cable via a coaxial switching device. After charging and energy storage, the output of the coaxial cable serves as the input to the coaxial switching device, and is output to ground via a discharge switch and matching resistor, forming a square wave current. Preferably, the 96-hole plate 2 is made of optically transparent pure polystyrene. To ensure a steep rising edge and high repetition rate output for the nanosecond signal, a mercury switch is used as the discharge switch. This mercury switch is installed in the coaxial device to ensure that the output signal is not distorted. The amplitude of the output magnetic field can be changed by altering the charging voltage amplitude. The duration of the output magnetic field can be continuously adjusted by changing the transmission line length. The repetition frequency of the output magnetic field can be adjusted by changing the switching frequency of the mercury switch.

[0093] A third aspect of the present invention provides a method for acquiring cell response characteristic data, utilizing the aforementioned nanosecond-level magnetic field irradiation device, the method comprising:

[0094] Cells were cultured in 96-well plates. Coaxial cable 3 was charged; after charging, the output of coaxial cable 3 was used as the input to a coaxial switch, generating a square wave current and ensuring that the cells in each row of wells of the 96-well plate received the same magnetic field irradiation intensity. Using magnetic field amplitude, duration, and frequency as independent variables, response characteristic data of experimental cells in the wells of the 96-well plate 2 under different electromagnetic field parameters with the same magnetic field irradiation intensity were obtained.

[0095] The nanosecond-level magnetic field irradiation device obtained through data acquisition methods can achieve continuous adjustment of magnetic field amplitude, repetition frequency, and duration, which is of great significance in making up for the lack of analysis of changes in the electromagnetic environment in organisms caused by changes in magnetic field parameters in current research.

[0096] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0097] It should also be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. To avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

[0098] Furthermore, various different embodiments of the present invention can be combined in any way, as long as they do not violate the spirit of the present invention, they should also be regarded as the content disclosed by the present invention.

Claims

1. A method for designing a nanosecond magnetic field irradiation device, characterized in that, The nanosecond magnetic field irradiation device design method comprises: S1, selecting different magnetic field generating devices according to the pulse width requirements of different currents; S2, arranging the magnetic field irradiation above the 96-hole plate (2) so that the magnetic field irradiation intensity on the cells cultured in each hole of the 96-hole plate (2) is equal; S3, building a nanosecond current source according to the requirements of nanosecond square waves; The nanosecond irradiation device design method firstly selects different magnetic field generating devices according to the pulse width requirements of different currents, secondly determines the distance between two holes of the 96-hole plate, and the obtained 96-hole plate makes the magnetic field irradiation intensity on the cells in each hole equal, and finally builds a nanosecond current source according to the requirements of square waves; The specific design method is as follows: The long straight wire with length of 2L is selected as the magnetic field generating device, and the current is The magnetic induction intensity of any point outside the long straight wire is: ，（1） In formula (1), is half of the length of the long straight wire, is the magnetic induction strength of any point outside the long straight wire, is the relative distance between the midpoint of the long straight wire and the projection of the measured point on the long straight wire, is the magnetic permeability of vacuum, is the distance of the current source; The reflection coefficient β and the refractive coefficient α of the charging terminal A point and the discharging terminal B point in the transmission line circuit are calculated as follows. Since the charging resistance value selected is infinitely large with respect to the load impedance , it is considered that the A point is in an open circuit state, and thus the reflection coefficient β and the refractive coefficient α of the A point are as follows: The reflection coefficient of point B and the refraction coefficient are: ，（10） wherein: is the reflection coefficient, is the refractive coefficient, is the load impedance, is the characteristic impedance of a lossless uniform transmission line; The oscillation amplitude of the output pulse is mainly generated by the first reflection, as long as the first oscillation amplitude is not greater than 10% of the impact peak value, and the condition for satisfying the square wave oscillation is ，（11） Therefore, ，（12） As long as the load impedance satisfies formula (12), a pulse square wave can be generated by using a uniform transmission line, and after the switch is turned on, the square wave pulse amplitude obtained on the load is half of the charging voltage, and the pulse width is twice the propagation time of the electromagnetic wave in the transmission line.

2. The nanosecond-scale magnetic field irradiation device design method according to claim 1, characterized in that, In S2: the long straight wire is a coaxial cable (3), and the coaxial cable (3) with the same length as the width of the 96-hole plate is covered on the 96-hole plate (2) so that the magnetic field irradiation intensity on the cells in each hole is equal.

3. The nanosecond-scale magnetic field irradiation device design method of claim 1, wherein, S3 comprises: calculating the general solution of the wave equation of the lossless uniform transmission line according to the telegraph equation of the lossless uniform transmission line, and then obtaining the relationship between the nanosecond voltage wave and the current wave in the lossless uniform transmission line from the general solution, （8） where: is the position on the transmission line is the total current at time , is the characteristic impedance of the lossless uniform transmission line, is the incident voltage wave propagating in the direction, is the reflected voltage wave propagating in the direction, is the wave propagation speed.

4. The nanosecond magnetic field irradiation device obtained by the method according to any one of claims 1-3, characterized in that, The nanosecond magnetic field irradiation device further comprises: a grounding copper plate (4) and a nanosecond square wave generator (1) arranged on the upper end face of the grounding copper plate (4); and the 96-hole plate (2) is arranged on the upper end face of the grounding copper plate (4). The main circuit of the nanosecond square wave generator (1) comprises a PLC, a high-voltage source, a current-limiting resistor, a coaxial cable (3), a switch and a matching impedance. The coaxial cable (3) is covered on the upper end face of the 96-hole plate (2) and has the same length as the width of the 96-hole plate (2).

5. The nanosecond-scale magnetic field irradiation device of claim 1, wherein, The 96-hole plate (2) is made of optically transparent pure polystyrene.

6. The nanosecond pulsed magnetic field apparatus of claim 4, wherein, The switch is a mercury switch.

7. A cell response characteristic data acquisition method using the nanosecond magnetic field irradiation device according to any one of claims 4-6, the data acquisition method comprising: The cells are adherently cultured in the 96-hole plate (2); The coaxial cable (3) is charged, and after the charging energy storage is completed, the output of the coaxial cable (3) is used as the input of the coaxial switch device to form a square wave current, and the magnetic field irradiation intensity received by the cells in each column of holes on the 96-hole plate (2) is the same; Taking the magnetic field amplitude, the magnetic field action time and the magnetic field action frequency as the independent variable parameters, the response characteristic data of the test cells in the holes of the 96-hole plate (2) under the action of electromagnetic fields with different parameters are obtained.

8. The data acquisition method according to claim 7, wherein: The magnetic field amplitude of the output magnetic field is changed by changing the amplitude of the charging voltage; The magnetic field action time is adjusted by changing the length of the long straight wire. The frequency of the magnetic field is adjusted by changing the on-off frequency of the discharge switch.

Images