A composition containing phytosphingosine and an oral care product

Abstract

The present application provides a composition containing phytosphingosine, the composition comprising a nonionic surfactant and phytosphingosine, wherein the phytosphingosine is present in the form of phytosphingosine inclusions, and the particle size of the phytosphingosine inclusions is less than or equal to 1000nm. The present application also provides an oral care product containing the composition. In the composition and oral care product of the present application, phytosphingosine is present in the form of phospholipid inclusions, and by compounding with a nonionic surfactant of a specific molecular weight, good cleaning and antibacterial effects can be achieved at the same time.

Description

Technical Field

[0001] The present application belongs to the field of cosmetics, and in particular to a composition containing phytosphingosine and an oral care product. Background Art

[0002] Mouthwash has two main functions: (1) cleaning the oral cavity, and (2) inhibiting the excessive growth of bacteria in the oral cavity. Among them, the cleaning effect is mainly contributed by the surfactant in the formula, while the antibacterial effect is achieved by adding antibacterial agents. In recent years, with consumers' preference for natural ingredients, traditional chemical antibacterial agents, such as CPC / domiphene / chlorhexidine, have gradually been replaced by biological antibacterial agents, among which the most representative biological antibacterial agent is "phytosphingosine". However, due to the application difficulties of phytosphingosine such as high melting point, insolubility in water, easy crystallization and precipitation, and poor absorption, phytosphingosine is usually used in the form of lecithin encapsulation in mouthwash, toothpaste and other formulas. On the one hand, it solves the application difficulties of phytosphingosine, and on the other hand, it increases the deposition of sphingosine in the oral cavity. During the use of oral care products, more than 99% of the material will be spit out by the user. Therefore, the active ingredient needs to have a high deposition property to remain in the oral cavity as much as possible to exert its effect. Summary of the invention

[0003] However, we have found in our research that when phospholipid-encapsulated phytosphingosine and a surfactant are used simultaneously, the antibacterial and cleaning properties decrease. To address this problem, the present invention provides a composition containing phytosphingosine, which can effectively balance the antibacterial and cleaning properties and maintain a uniform and transparent appearance, thereby completing the present invention.

[0004] Specifically, this application involves the following aspects:

[0005] 1. A composition containing phytosphingosine, characterized in that the composition comprises a nonionic surfactant and phytosphingosine, wherein the phytosphingosine exists in the form of phytosphingosine inclusion bodies.

[0006] 2. The composition containing phytosphingosine according to item 1, characterized in that the mass ratio of the nonionic surfactant to the phytosphingosine is 0.5-150:1, preferably 20-100:1.

[0007] 3. The composition containing phytosphingosine according to item 1 is characterized in that, in the composition, the mass content of the phytosphingosine is 0.005%-0.2%, preferably 0.008%-0.02%, and the mass content of the non-ionic surfactant is 0.05%-2%, preferably 0.1%-1%.

[0008] 4. The composition containing phytosphingosine according to item 1, characterized in that the molecular weight of the non-ionic surfactant is greater than 3000Da, preferably greater than 5000Da.

[0009] 5. The composition containing phytosphingosine according to item 1, characterized in that the nonionic surfactant is selected from one or more of polyoxyethylene / polyoxypropylene (EO / PO) copolymers, polysorbates, PEG-N hydrogenated castor oils, glucosides, polyglycerol fatty acid esters, sorbitan fatty acid esters, and polyethers;

[0010] Preferably, the polyoxyethylene / polyoxypropylene (EO / PO) copolymer is selected from poloxamers, and further selected from one or more of poloxamer 101, poloxamer 124, poloxamer 182, poloxamer 184, poloxamer 185, poloxamer 188, poloxamer 215, poloxamer 217, poloxamer 234, poloxamer 235, poloxamer 237, poloxamer 238, poloxamer 331, poloxamer 338, and poloxamer 407;

[0011] The polysorbates are selected from one or both of polysorbate-20 and polysorbate-80;

[0012] The PEG-N hydrogenated castor oil is selected from one or both of PEG-40 hydrogenated castor oil and PEG-60 hydrogenated castor oil;

[0013] The glucosides are selected from C10-16 alkyl glucosides;

[0014] The polyglycerol fatty acid esters are selected from one or both of polyglycerol-10 laurate and polyglycerol-5 myristate;

[0015] The sorbitan fatty acid esters are selected from sorbitan oleate;

[0016] The polyethers are selected from one or more of beheneth-30, steareth-30 and steareth-5.

[0017] 6. The composition containing phytosphingosine according to item 1, characterized in that the HLB of the non-ionic surfactant is greater than 5.

[0018] 7. The composition containing phytosphingosine according to item 1, characterized in that the composition further comprises other surfactants besides non-ionic surfactants.

[0019] 8. The composition containing phytosphingosine according to item 1, characterized in that the phytosphingosine inclusion body is phytosphingosine wrapped by a lipid substance, preferably phytosphingosine wrapped by a phospholipid, and more preferably phytosphingosine wrapped by lecithin;

[0020] Preferably, the particle size of the phytosphingosine inclusions is less than or equal to 1000 nm.

[0021] 9. An oral care product, characterized in that the oral care product comprises the composition containing phytosphingosine described in any one of items 1-8.

[0022] 10. The oral care product according to item 9, further comprising one or more of a moisturizer, a sweetener, a preservative, a flavor, a pH adjuster, a cooling agent, and a pigment.

[0023] 11. The oral care product according to item 10, which is a mouthwash or a toothpaste.

[0024] Beneficial Effects

[0025] The present application uses a compound of phytosphingosine and a nonionic surfactant to effectively balance the antibacterial performance brought by phytosphingosine and the cleaning performance brought by the surfactant, and has a good uniform and transparent appearance; even if other types of surfactants are added, the antibacterial performance and the cleaning performance can be achieved at the same time. DETAILED DESCRIPTION

[0026] The present application is further described below in conjunction with examples. It should be understood that the examples are only used to further describe and illustrate the present application and are not used to limit the present application.

[0027] Unless otherwise defined, the technical and scientific terms in this specification have the same meaning as those generally understood by those skilled in the art. Although similar or identical methods and materials as described herein may be used in experiments or practical applications, materials and methods are described herein below. In the event of a conflict, the present specification including the definitions therein shall prevail. In addition, materials, methods and examples are provided for illustration only and are not restrictive. The present application is further described below in conjunction with specific embodiments, but is not intended to limit the scope of the present application.

[0028] The present application provides a composition containing phytosphingosine, wherein the composition comprises a nonionic surfactant and phytosphingosine, wherein the phytosphingosine exists in the form of phytosphingosine inclusion bodies.

[0029] Among them, nonionic surfactants are surfactants that contain ether groups that do not dissociate in aqueous solution as the main hydrophilic group in the molecule, and their surface activity is reflected by neutral molecules. Nonionic surfactants have high surface activity, good solubilization, washing, antistatic, calcium soap dispersion and other properties, low irritation, and excellent wetting and washing functions. The applicable pH range is wider than that of general ionic surfactants, and can also be used with other ionic surfactants. Adding a small amount of nonionic surfactants to ionic surfactants can improve the surface activity of the system. Nonionic surfactants can be divided into polyoxyethylene type, polyol type, alkanolamide type, polyether type, amine oxide type, etc. according to the structure of the hydrophilic group.

[0030] In a specific embodiment, the molecular weight of the nonionic surfactant is not limited, for example, it can be greater than 3000 Da, preferably greater than 5000 Da. For example, it may be 3100 Da, 3500 Da, 4000 Da, 4500 Da, 5000 Da, 5500 Da, 6000 Da, 6500 Da, 7000 Da, 7500 Da, 8000 Da, 9000 Da, 10000 Da, 10500 Da, 11000 Da, 11500 Da, 12000 Da, 12500 Da, 13000 Da, 13500 Da, 14000 Da, 14500 Da, 15000 Da, 15500 Da, 16000 Da, 16500 Da, 17000 Da, 17500 Da, 18000 Da, 18500 Da, 19000 Da, 19500 Da, or 20000 Da.

[0031] In a specific embodiment, the HLB of the nonionic surfactant is greater than 5. For example, it can be 5.1, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 15.5, 16, 16.5, 17, 17.5, 18, 18.5, 19, 19.5, 20, 20.5, 21, 21.5, 22, 22.5, 23, 23.5, 24, 24.5, 25.

[0032] Among them, the HLB value (Hydrophile-Lipophile Balance Number) is called the hydrophilic-hydrophobic balance value, also known as the water-oil ratio. In 1949, WC Griffin first proposed the HLB value argument to explain the balance between the hydrophilic group and the lipophilic group in the surfactant molecule. In HLB, H "Hydrophile" means hydrophilicity, L "Lipophilic" means lipophilicity, and B "Balance" means balance.

[0033] HLB = hydrophilicity of the hydrophilic group / lipophilicity of the lipophilic group.

[0034] The lipophilicity or hydrophilicity of a surfactant can be determined by the size of the HLB value. The larger the HLB value, the stronger the hydrophilicity, and the smaller the HLB value, the stronger the lipophilicity. Generally speaking, the HLB value ranges from 1 to 40. It is related to the hydrophilicity and lipophilicity of the surfactant, as well as the basic properties of the surfactant such as surface (interfacial) tension, adsorption on the interface, emulsification and emulsion stability, dispersibility, solubility, and detergency. It is also related to the application performance of the surfactant. HLB has important reference value in practical applications. The HLB of lipophilic surfactants is lower, and the HLB of hydrophilic surfactants is higher.

[0035] In a specific embodiment, the nonionic surfactant is selected from one or more of polyoxyethylene / polyoxypropylene (EO / PO) copolymers, polysorbates, PEG-N hydrogenated castor oils, glucosides, polyglycerol fatty acid esters, sorbitan fatty acid esters, and polyethers;

[0036] Preferably, the polyoxyethylene / polyoxypropylene (EO / PO) copolymer is selected from poloxamers, and further selected from one or more of poloxamer 101, poloxamer 124, poloxamer 182, poloxamer 184, poloxamer 185, poloxamer 188, poloxamer 215, poloxamer 217, poloxamer 234, poloxamer 235, poloxamer 237, poloxamer 238, poloxamer 331, poloxamer 338, and poloxamer 407;

[0037] The polysorbates are selected from one or both of polysorbate-20 and polysorbate-80;

[0038] The PEG-N hydrogenated castor oil is selected from one or both of PEG-40 hydrogenated castor oil and PEG-60 hydrogenated castor oil;

[0039] The glucosides are selected from C10-16 alkyl glucosides;

[0040] The polyglycerol fatty acid esters are selected from one or both of polyglycerol-10 laurate and polyglycerol-5 myristate;

[0041] The sorbitan fatty acid esters are selected from sorbitan oleate;

[0042] The polyethers are selected from one or more of beheneth-30, steareth-30 and steareth-5.

[0043] In a specific embodiment, the composition further comprises other surfactants besides the non-ionic surfactant, and the other surfactants comprise one or more of cationic surfactants, anionic surfactants and amphoteric surfactants.

[0044] The cationic surfactant includes polyquaternium salts, for example, polyquaternium-4, polyquaternium-7, polyquaternium-10, polyquaternium-49, etc.

[0045] The anionic surfactants include sulfonates, sulfate esters, and bile salts. For example, the sulfates may be sodium lauryl sulfate, sodium dodecyl sulfate, etc., the sulfonates may be sodium dodecylbenzenesulfonate, etc., and the bile salts may be sodium glycocholate, etc.

[0046] The zwitterionic surfactant includes an amino acid type and a betaine type. For example, the amino acid type may be lauroyl ammonium chloride, cocoyl glutamate, etc., and the betaine type may be cocamidopropyl betaine, etc.

[0047] In a specific embodiment, the mass ratio of the other surfactant to the nonionic surfactant is not limited. In the present application, for example, the mass ratio of the other surfactant to the nonionic surfactant is less than 1:50 and greater than 0, preferably 1:(50-500).

[0048] In a specific embodiment, the amount of other surfactants added is not limited. In the present application, the amount of other surfactants added is less than the amount of nonionic surfactants added. For example, based on the total mass in the composition, the amount of other surfactants added can be greater than 0 and less than 0.4%, preferably 0.00001%-0.1%.

[0049] Phytosphingosine is a precursor of ceramide and one of the lipid components of the skin. It has a natural barrier repair function. It also has a strong inhibitory effect on microbial activity and can inhibit harmful microorganisms such as Propionibacterium acnes, Staphylococcus aureus, and Malassezia. Phytosphingosine effectively fights inflammation and removes redness by inhibiting the synthesis of prostaglandins. As one of the lipid components of the skin, phytosphingosine has multiple effects on the skin, adding points to high-end products such as anti-aging and repair.

[0050] Wherein, the phytosphingosine inclusion body refers to phytosphingosine wrapped by lipid substances. In a specific embodiment, the phytosphingosine inclusion body is phytosphingosine wrapped by phospholipids. The phospholipids are glycerophospholipids, preferably lecithin.

[0051] The particle size of the phytosphingosine inclusions is less than or equal to 1000nm, and the particle size is the D90 particle size, that is, the particle size corresponding to when the cumulative particle size distribution number of a sample reaches 90%, and its physical meaning is that the particles with a particle size smaller than it account for 90%. For example, it can be 1nm, 2nm, 3nm, 4nm, 5nm, 6nm, 7nm, 8nm, 9nm, 10nm, 11nm, 12nm, 13nm, 14nm, 15nm, 16nm, 17nm, 18nm, 19nm, 20nm, 21nm, 22nm, 23nm, 24nm, 25nm, 26nm, 27nm, 28nm, 29nm, 30nm, 35nm, 40nm, 45nm, 50nm, nm, 55nm, 60nm, 65nm, 70nm, 75nm, 80nm, 85nm, 90nm, 95nm, 100nm, 150nm, 200nm, 250nm, 300nm, 350 nm, 400nm, 450nm, 500nm, 550nm, 600nm, 650nm, 700nm, 750nm, 800nm, 850nm, 900nm, 950nm, 1000nm.

[0052] The phytosphingosine inclusion bodies can be purchased commercially or prepared by known techniques.

[0053] In a specific embodiment, the above-mentioned phytosphingosine inclusions are prepared by the following method:

[0054] The phospholipid, phytosphingosine and oil are mixed to obtain an oil phase system;

[0055] Mixing polyol and water to obtain an aqueous phase system;

[0056] Adding the aqueous phase system to the oil phase system, dispersing and homogenizing, to obtain phytosphingosine inclusions;

[0057] Furthermore, the oil is one or both of liquid lipids and solid lipids.

[0058] Furthermore, based on the total mass of the oil phase system and the aqueous phase system, the mass proportion of the phospholipids is 0.1-10%, the mass proportion of the phytosphingosine is 0.1-20%, the mass proportion of the liquid lipids is 0-10%, the mass proportion of the solid lipids is 0-10%, and the mass proportion of the polyols is 0-90%.

[0059] In a specific embodiment, in the composition, the mass content of the phytosphingosine is 0.005%-0.2%, preferably 0.008%-0.02%, for example, it can be 0.005%, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%. , 0.17%, 0.18%, 0.19%, 0.2%; the mass content of the nonionic surfactant is 0.05%-2%, preferably 0.1%-1%, for example, it can be 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%.

[0060] In a specific embodiment, the mass ratio of the nonionic surfactant to the phytosphingosine is 0.5-150:1, preferably 20-100:1, for example, it can be 0.5:1, 1:1, 2:1, 5:1, 10:1, 15:1, 20:1, 25:1, 30:1, 35:1, 40:1, 45:1, 50:1, 55:1, 60:1, 65:1, 70:1, 75:1, 80:1, 85:1, 90:1, 95:1, 100:1, 105:1, 110:1, 115:1, 120:1, 125:1, 130:1, 135:1, 140:1, 145:1, 150:1.

[0061] The present application also provides an oral care product comprising the above-mentioned composition containing phytosphingosine. Further, the oral care product may also include one or more of a moisturizer, a sweetener, a preservative, a flavor, a pH adjuster, a cooling agent, and a pigment.

[0062] The oral care product may be a mouthwash or a toothpaste.

[0063] In a specific embodiment, the oral care product is a mouthwash.

[0064] The preparation method of the mouthwash is not limited in this application, for example, it can be prepared by the following steps:

[0065] Adding water-soluble substances such as moisturizers, sweeteners, preservatives, phytosphingosine inclusions, and nonionic surfactants to water, stirring until the materials are completely dissolved, to obtain an aqueous phase system;

[0066] Adding oil-soluble substances such as cooling agents (e.g., menthol) to the flavor to prepare an oil phase system;

[0067] Under rapid stirring, the oil phase is added to the water phase system, and the two phases are rapidly stirred until the two phases are evenly mixed and the liquid is clear and transparent to obtain a mouthwash.

[0068] The present application also provides use of the above-mentioned composition containing phytosphingosine in preparing oral care products.

[0069] Example

[0070] Example 1

[0071] Add 1g of poloxamer 407 to water and stir until the poloxamer 407 is completely uniform. Then add 0.1g of phytosphingosine inclusions (commercially available product / Pure Biopharmaceutical Technology Co., Ltd., batch number 20220101, the phytosphingosine content in the inclusions is 10%), where the total mass of the entire system is 100g, and stir for 5 minutes until it is completely dissolved.

[0072] The molecular weight of poloxamer 407 is about 12,500 and the HLB value is about 22.5.

[0073] Embodiment 2-7

[0074] The difference between Examples 2-7 and Example 1 is the selection or dosage of the surfactant.

[0075] Specifically, the difference between Example 2 and Example 1 is only that the amount of surfactant added is different, which is 0.4 g.

[0076] The only difference between Example 3 and Example 1 is the amount of surfactant added, which is 1.5 g.

[0077] The difference between Example 4 and Example 1 is that the type of surfactant added is different, which is polysorbate 20. The molecular weight of polysorbate 20 is about 1228, and the HLB value is about 16.7.

[0078] The difference between Example 5 and Example 1 is that the type of surfactant added is different, which is PEG-40 hydrogenated castor oil. The molecular weight of PEG-40 hydrogenated castor oil is about 2700, and the HLB value is about 15.

[0079] The difference between Example 6 and Example 1 is that the type of surfactant added is different, which is PEG-60 hydrogenated castor oil. The molecular weight of PEG-60 hydrogenated castor oil is about 3580, and the HLB value is about 16.

[0080] Example 7

[0081] The difference between Example 7 and Example 1 is that 0.005% of polyquaternium-4 is added to Example 1.

[0082] Comparative Example 1

[0083] The difference between Comparative Example 1 and Example 1 is that the type of surfactant added is different, namely sodium dodecyl sulfate, wherein the molecular weight of sodium dodecyl sulfate is about 288.38 and the HLB value is about 40.

[0084] Comparative Example 2

[0085] The difference between Comparative Example 2 and Example 1 is that the type of surfactant added is different, which is polyquaternium-4. The molecular weight of polyquaternium-4 is about 123, and the HLB value is about 15.8.

[0086] The compositions prepared in the above Examples 1-7 and Comparative Example 1 are uniform. However, the composition obtained in Comparative Example 2 is not transparent and uniform, which does not meet the requirements for product marketing. Therefore, no further efficacy test was performed on Comparative Example 2.

[0087] The dosage and parameters of each component in the above embodiment are specifically shown in Table 1.

[0088] Table 1

[0089]

[0090]

[0091] Test Example 1 Antibacterial Performance Test

[0092] Test method:

[0093] A) Dilute the test bacteria (Staphylococcus aureus and Escherichia coli) suspension appropriately with PBS solution. The required concentration is: take 0.1 mL and drop it into 5.0 mL of control sample solution (PBS). The number of recovered bacteria is 1×10 4 ~9×10 4 cfu / mL;

[0094] B) 5.0 mL of the test sample stock solution (samples prepared in Examples 1 to 7 and Comparative Example 1, 0.01% phytosphingosine) was taken into a sterilized test tube and kept at 20° C. for 5 min (30° C. for soap products);

[0095] C) Pipette 0.1 mL of the test bacterial solution and add it to the test tube containing 5.0 mL of the sample, mix quickly, and start the timer immediately;

[0096] D) After the set time, take 0.5 mL of the test bacteria and sample mixture and add it to a test tube containing 4.5 mL of sterilized PBS and mix thoroughly;

[0097] E) After 10 minutes of standing, take 1 mL of the sample solution (or after appropriate dilution, take 2-3 dilutions) and place it in a sterile plate. Inoculate two sterile plates for each sample solution or dilution. Pour 15 mL of nutrient agar medium (bacteria) or Sabouraud agar medium (candida albicans) cooled to 40℃-45℃, rotate the plate to make it fully uniform, turn the plate over after the agar solidifies, and culture at (35+2)℃ for 48h (bacteria) or 72h (candida albicans), and count the number of live bacteria.

[0098] F) Substituting PBS for the test sample and following the above steps as the control sample;

[0099] G) Repeat the experiment 3 times and calculate the average value.

[0100] H) Calculate the inhibition rate, the results are shown in Table 2.

[0101] The antibacterial rate was calculated according to the following formula:

[0102] Antibacterial rate (%) = (Ⅰ-Ⅱ) / Ⅰ*100

[0103] Where:

[0104] Ⅰ——average colony count of control samples;

[0105] Ⅱ——Average colony count of test samples.

[0106] Evaluation of antibacterial effect: an inhibition rate of >90% indicates that the product has a strong antibacterial effect, and an inhibition rate of >50-90% indicates that the product has an antibacterial effect.

[0107] Table 2

[0108]

[0109] Experimental conclusion: The present embodiment of the present invention can have a strong antibacterial effect on Staphylococcus aureus and / or Escherichia coli (antibacterial rate>90%) by using phytosphingosine and non-ionic surfactant in combination. However, since Comparative Example 1 does not use non-ionic surfactant, it cannot achieve a strong antibacterial effect on either Staphylococcus aureus or Escherichia coli.

[0110] Test Example 2 Cleaning Performance Test

[0111] Test method:

[0112] 1. Experimental instruments and reagents

[0113] Tooth mold (ordinary dental medical model (8cm*6cm), purchased from Dezhou Medical Equipment Store), light box with adjustable light intensity, magnetic stirrer (Hei-connect model, purchased from Heidolf, Germany), rotor (Z25 magnetic stirrer, purchased from Titan Exploration Platform), camera (2592*1936 pixel resolution / 72dpi), 1L standard stainless steel container (purchased from Titan Platform), mouthwash (mouthwash prepared in Examples 1-6 of the present application and Comparative Example 1, commercially available mouthwash (Colgate Mouthwash (Jasmine Green Tea Flavor)), cassava starch / corn starch (Tapioca Pure, purchased from Nouryon (AKZO NOBEL) Company), carbon powder (TR6860, purchased from Shanghai Fu Rui Chemical).

[0114] 2. Experimental plan

[0115] 1) Configure simulated dirt

[0116] Take 4g of cassava starch, add 0.01g of carbon powder (for dyeing), mix well, add 3g of deionized water, and adjust into a slurry for later use.

[0117] 2) Standard cleaning process, standard photo taking process and standard contamination process for dental molds.

[0118] 1>Standard cleaning process:

[0119] Take the tooth mold and use a toothbrush (Colgate charcoal fine-bristled gum protection toothbrush) with a soft-bristle brush head dipped in sodium lauryl sulfate (toothpaste-grade sodium lauryl sulfate, purchased from Shanghai Youyang Industrial) to carefully brush and clean it. Wash the tooth mold carefully with clean water until it is completely clean, then carefully wipe it dry with absorbent paper and set aside.

[0120] 2>Standard photography process

[0121] Place the light box on a horizontal laboratory table and mark its center position. The direction parallel to the long side of the light box when it is placed horizontally is defined as the X-axis, the direction parallel to its short side is defined as the Y-axis, and the intersection of the X-axis and the Y-axis is defined as the center position mentioned above. Open the tooth model horizontally and place it on a horizontal laboratory table. The center of the model coincides with the center of the light box, and the long axis running through the center of the upper and lower incisors of the tooth model coincides with the X-axis, and this position is set as the standard model placement position.

[0122] Use a camera with a resolution of 2592*1936 pixels / 72dpi to shoot at the shooting port above the light box. When shooting, make the tooth model occupy a certain number of pixels along the X-axis and Y-axis directions as required. And fix the camera mode, which is the standard shooting procedure.

[0123] Before shooting, adjust the brightness of the ambient light inside the light box so that the brightness of the selected teeth is between 80-95 (the tooth whiteness is measured by the L value in the LAB color space of the tooth surface in the camera). Select 32 sampling points on the selected teeth, and set the ambient brightness so that the difference between the highest and lowest L values ​​of the teeth at each sampling point does not exceed 5, and then fix the ambient light as the standard ambient light.

[0124] After the shooting is completed, the teeth of the upper jaw and the teeth of the lower jaw are taken, and then the L values ​​thereof are sampled (the L value sampling method is shown below in the L value sampling method).

[0125] 3>L value standard sampling method

[0126] In order to convert the acquired digital photo from RGB color space to Lab color space, first convert the RGB color space into color tristimulus values ​​X, Y, and Z, and then calculate the chromaticity value of the Lab color space according to the calculation formula of CIE 1976 uniform color space Lab. Extract the L value of each pre-selected sampling point (see above for details) and record it.

[0127] 4>Standard pollution method

[0128] For the upper and lower gums of the dental mold, take 0.3 g of the simulated dirt slurry prepared above, apply it evenly on the mold and set aside.

[0129] 3) Standard test process for samples to be tested

[0130] 1> Establish the L value standard before the mold is cleaned

[0131] For the dental mold that has undergone the standard cleaning process as described above, take a photo according to the standard photo process, and record the L value of each sampling point as the L value of the measurement point before applying dirt.

[0132] Take a 1L standard stainless steel container, add 250ml of various mouthwashes of the present application and commercially available mouthwash respectively (make sure the liquid level is above the upper and lower tooth surfaces of the mold), place a magnetic stirrer in the tooth mold, and then place the tooth mold in a standard stainless steel container, place it on a magnetic stirrer, use 600RPM magnetic stirring for 30s (start timing after it reaches the specified speed), take it out, use clean water to gently rinse off the large stains remaining on the mold, use absorbent paper to gently absorb the residual water on the surface, take pictures according to the standard shooting process described above, and sample the L value of each sampling point according to the sampling method described above and record it as the L value of the measurement point after using this round of test sample liquid.

[0133] 4) Analysis and comparison

[0134] The L value is used to evaluate the washing rate of the sample. The formula is:

[0135] Cleaning rate of a single point = 1-(L value of the measurement point before applying dirt-L value after cleaning with sample) / L value of the measurement point before applying dirt*100%

[0136] Average cleaning rate = (the sum of the cleaning rates of each measuring point) / the number of selected measuring points

[0137] The test results of the average cleaning rate are shown in Table 3.

[0138] Table 3

[0139] Average cleaning rate Example 1 90.41％ Example 2 88.75％ Example 3 84.12％ Example 4 88.07％ Example 5 87.25％ Example 6 83.66％ Example 7 89.32％ Comparative Example 1 88.89％ Commercially available mouthwash 84.56％

[0140] The results in Table 3 show that the composition containing phytosphingosine prepared in the present application can achieve or even exceed the cleaning ability of commercially available mouthwashes, and therefore can meet the requirements of product cleaning performance.

Claims

1. A composition containing phytosphingosine, characterized in that The composition comprises a nonionic surfactant and phytosphingosine, wherein the nonionic surfactant is poloxamer 407, and the phytosphingosine exists in the form of phytosphingosine inclusion bodies; The mass ratio of the poloxamer 407 to the phytosphingosine is 40-150:1; The phytosphingosine inclusion body is phytosphingosine wrapped by lipid substances; The mass ratio of other surfactants to the nonionic surfactant is less than 1:

50.

2. The composition containing phytosphingosine according to claim 1, characterized in that The mass ratio of the poloxamer 407 to the phytosphingosine is 40-100:

1.

3. The composition containing phytosphingosine according to claim 1, characterized in that In the composition, the mass content of phytosphingosine is 0.005%-0.2%, and the mass content of poloxamer 407 is 0.05%-2%.

4. The composition containing phytosphingosine according to claim 3, characterized in that In the composition, the mass content of the phytosphingosine is 0.008%-0.02%.

5. The composition containing phytosphingosine according to claim 3, characterized in that In the composition, the mass content of Poloxamer 407 is 0.1%-1%.

6. The composition containing phytosphingosine according to claim 1, characterized in that The phytosphingosine inclusion body is phytosphingosine encapsulated by phospholipids.

7. The composition containing phytosphingosine according to claim 1, characterized in that The phytosphingosine inclusion body is phytosphingosine encapsulated by lecithin.

8. The composition containing phytosphingosine according to claim 1, characterized in that The particle size of the phytosphingosine inclusions is less than or equal to 1000 nm.

9. An oral care product, characterized in that: The oral care product comprises the composition containing phytosphingosine according to any one of claims 1 to 8.

10. The oral care product according to claim 9, further comprising one or more of a moisturizer, a sweetener, a preservative, a flavor, a pH adjuster, a cooling agent, and a pigment. The oral care product according to claim 10 , which is a mouthwash or a toothpaste.

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