In-vitro embryo culture dish and culture device

Abstract

The application discloses an embryo in-vitro culture dish and a culture device. At least one side of the embryo in-vitro culture dish is provided with a mounting position for connecting a bottle mouth of a culture container. The embryo in-vitro culture dish is internally provided with an air inlet channel and an air outlet channel. The outlet of the air inlet channel and the inlet of the air outlet channel are both located at the mounting position, so that external gas can enter the culture container through the air inlet channel, and the gas in the culture container can be discharged outward through the air outlet channel. Compared with the prior art, the embryo in-vitro culture dish and the culture device can improve the gas exchange efficiency of the culture container.

Description

Technical Field

[0001] This invention relates to the field of embryo culture technology, and in particular to a rotating embryo in vitro culture tray and culture device. Background Technology

[0002] In vitro embryo culture refers to the process of placing embryos in specialized culture containers to allow them to develop continuously outside the body. In existing technologies, horizontally positioned culture containers are typically mounted on vertically positioned rotating disks. The rotating disks contain gas channels, and corresponding to the positions of the culture containers are through-holes that communicate with these gas channels to allow for gas exchange within the culture containers. However, each culture container usually corresponds to only one through-hole, which is used for both gas intake and exhaust, resulting in low gas exchange efficiency. Summary of the Invention

[0003] The purpose of this invention is to provide an in vitro embryo culture dish and culture device to improve the gas exchange efficiency of the culture container.

[0004] To achieve the above objectives, the present invention provides the following solution:

[0005] This invention discloses an in vitro embryo culture tray, wherein at least one side of the tray has a mounting position for connecting to the mouth of a culture container; the in vitro embryo culture tray has an air inlet channel and an air outlet channel inside, wherein the outlet of the air inlet channel and the inlet of the air outlet channel are both located at the mounting position, so that external gas can enter the culture container through the air inlet channel and the gas inside the culture container can be discharged outward through the air outlet channel.

[0006] Preferably, the embryo culture tray has mounting positions on both the right and left sides; the embryo culture tray includes a first tray body, a second tray body, and a third tray body arranged sequentially from right to left.

[0007] The first disc body is provided with a first disc body through hole I, a first disc body through hole II, a first disc body through hole III and a first disc body through hole IV, and a first disc body groove I is provided on the left side of the first disc body; the first disc body groove I connects the first disc body through hole II and the first disc body through hole III; the right end of the first disc body through hole III and the right end of the first disc body through hole IV are directly opposite the mounting position located on the right side;

[0008] The second disk body is provided with a second disk body through hole I, a second disk body through hole II, and a second disk body through hole III. The left side of the second disk body is provided with a second disk body groove I, and the right side of the second disk body is provided with a second disk body groove II. The second disk body through hole I passes through the second disk body groove I. The second disk body groove II connects the second disk body through hole II and the second disk body through hole III. The first disk body through hole IV is directly opposite the second disk body groove II.

[0009] The third disk body is provided with a third disk body through hole I, and the third disk body through hole I is directly opposite to the second disk body through hole III;

[0010] The mounting position on the left side is directly opposite to a portion of the groove I of the second disc and the position of the through hole II of the second disc, through the through hole of the third disc body.

[0011] Preferably, the in vitro embryo culture tray further includes a fourth tray located to the left of the third tray; the third tray has a third tray groove I on its left side, and a third tray through hole I passes through the third tray groove I; the fourth tray has a fourth tray through hole I and a fourth tray through hole II; the fourth tray through hole I is directly opposite the third tray groove I; the fourth tray through hole II allows the mouth of the culture container on the left side to pass through.

[0012] Preferably, the in vitro embryo culture tray further includes a right mounting tray and a left mounting tray; the right mounting tray is located on the right side of the first tray body, and the left mounting tray is located on the left side of the fourth tray body; the right mounting tray has a right mounting hole that is directly opposite to the right mounting position, and the right mounting hole is used to install the right culture container; the left mounting tray has a left mounting hole that is directly opposite to the left mounting position, and the left mounting hole is used to install the left culture container.

[0013] Preferably, both the right-side mounting hole and the left-side mounting hole are threaded holes.

[0014] Preferably, the in vitro embryo culture dish further includes an annular sealing plug, which includes a right sealing plug and a left sealing plug; one end of the right sealing plug contacts the right side of the first dish body, and the other end of the right sealing plug contacts the inner wall of the mouth of the culture container on the right side; one end of the left sealing plug contacts the left side of the third dish body, and the other end of the left sealing plug contacts the inner wall of the mouth of the culture container on the left side.

[0015] The present invention also discloses an in vitro embryo culture device, which includes the above-mentioned in vitro embryo culture tray, an air inlet pipe located on the right side of the first tray, and an air outlet pipe located on the left side of the fourth tray.

[0016] The left end of the air intake pipe has a first outer flange, which is fixedly connected to the first disc body. A first cavity is formed between the first outer flange and the first disc body. The first disc body through hole I and the first disc body through hole II are both connected to the first cavity.

[0017] The right end of the air outlet pipe has a second outer flange, which is fixedly connected to the fourth disc body. A second cavity is formed between the second outer flange and the fourth disc body, and the through hole I of the fourth disc body is connected to the first cavity.

[0018] The air inlet pipe, the air outlet pipe, and the embryo in vitro culture tray are arranged coaxially.

[0019] Preferably, the in vitro embryo culture tray further includes a fixing support and a pneumatic slip ring; the fixing support includes a right-side fixing support and a left-side fixing support, and the pneumatic slip ring includes a right-side pneumatic slip ring and a left-side pneumatic slip ring;

[0020] The intake pipe is rotatably mounted on the right fixed support, and the right pneumatic slip ring is mounted on the right end of the intake pipe; the exhaust pipe is rotatably mounted on the left fixed support, and the left pneumatic slip ring is mounted on the left end of the exhaust pipe.

[0021] Preferably, the in vitro embryo culture device further includes a driving device, which is connected to the air inlet pipe or the air outlet pipe to drive the in vitro embryo culture tray to rotate through the air inlet pipe or the air outlet pipe.

[0022] Preferably, the in vitro embryo culture apparatus further includes the culture container.

[0023] The present invention achieves the following technical effects compared to the prior art:

[0024] In this invention, the outlet of the air inlet channel and the inlet of the air outlet channel are both located at the installation position. The gas in the air inlet channel must pass through the culture container before entering the air outlet channel. This is equivalent to connecting the culture container in series with the gas passage inside the embryo in vitro culture plate, forcing the culture container to perform gas exchange, thereby improving the gas exchange efficiency. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is an exploded view of the in vitro embryo culture apparatus of this embodiment;

[0027] Figure 2 for Figure 1 A partial schematic diagram;

[0028] Figure 3 This is an exploded view of the in vitro embryo culture device in this embodiment from another perspective;

[0029] Figure 4 This is a schematic diagram of the in vitro embryo culture apparatus of this embodiment;

[0030] Explanation of reference numerals in the attached drawings: 1-Fixed support; 2-Drive motor; 3-Right-side pneumatic slip ring; 4-Cultivation container; 5-Right-side mounting plate; 6-Sealing plug; 7-Plate; 8-First plate; 81-First plate through hole I; 82-First plate through hole II; 83-First plate through hole III; 84-First plate through hole IV; 85-First plate groove I; 9-Second plate; 91-Second plate through hole I; 92-Second plate through hole IV; 93-Second disc through hole II; 94-Second disc groove I; 95-Second disc groove II; 10-Third disc; 101-Third disc through hole I; 102-Third disc groove I; 11-Fourth disc; 111-Fourth disc through hole I; 112-Fourth disc through hole II; 12-Left side mounting disc; 13-Left side pneumatic slip ring; 14-Outlet pipe; 15-Inlet pipe; 16-Transmission belt. Detailed Implementation

[0031] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] The purpose of this invention is to provide an in vitro embryo culture dish and culture device to improve the gas exchange efficiency of the culture container.

[0033] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. In this embodiment, left and right sides are used only to describe relative positions, not absolute positions. Figure 4 The direction of the middle arrow indicates the direction of gas flow.

[0034] Reference Figures 1-4This embodiment provides an in vitro embryo culture dish. At least one side of the dish has a mounting position for connecting to the mouth of a culture container 4. The in vitro embryo culture dish has an air inlet channel and an air outlet channel inside. The outlet of the air inlet channel and the inlet of the air outlet channel are both located at the mounting position, allowing external gas to enter the culture container 4 through the air inlet channel and gas inside the culture container 4 to exit through the air outlet channel. In use, the components of the in vitro embryo culture dish are relatively fixed and rotate together around the axis of the in vitro embryo culture dish.

[0035] In the prior art, the installation position of the culture container 4 corresponds to only one gas interface, and the culture container 4 is equivalent to being connected in parallel to the gas passage inside the embryo in vitro culture tray. Most of the gas entering the embryo in vitro culture tray does not enter the culture container 4, but flows directly out of the embryo in vitro culture tray along the gas passage, which results in very low gas exchange efficiency within the culture container 4.

[0036] In this application, the outlet of the air inlet channel and the inlet of the air outlet channel are both located at the installation position. The gas in the air inlet channel must pass through the culture container 4 before entering the air outlet channel. This is equivalent to connecting the culture container 4 in series with the gas passage inside the embryo in vitro culture plate, forcing the culture container 4 to perform gas exchange, thereby improving the gas exchange efficiency of the culture container 4.

[0037] To maximize the number of embryos cultured in a single run and reduce experimental time, this embodiment includes mounting positions on both the right and left sides of the embryo culture tray. The embryo culture tray comprises a first tray 8, a second tray 9, and a third tray 10 arranged sequentially from right to left.

[0038] The first disc body 8 is provided with first disc body through holes I 81, II 82, III 83, and IV 84. A first disc body groove I 85 is provided on the left side of the first disc body 8. The first disc body groove I 85 connects the first disc body through holes II 82 and III 83. The right ends of the first disc body through holes III 83 and IV 84 are directly opposite the mounting position located on the right side.

[0039] The second disc 9 has a second disc through hole I 91, a second disc through hole II 92, and a second disc through hole III 93. The left side of the second disc 9 has a second disc groove I 94, and the right side of the second disc 9 has a second disc groove II 95. The second disc through hole I 91 passes through the second disc groove I 94. The second disc groove II 95 connects the second disc through hole II 92 and the second disc through hole III 93. The first disc through hole IV 84 is directly opposite the second disc groove II 95.

[0040] The third disc body 10 is provided with a third disc body through hole I101, which is directly opposite to the second disc body through hole III93.

[0041] The mounting position on the left side is through a through hole in the third disc 10, and is directly opposite to a portion of the groove I 94 of the second disc and the through hole II 92 of the second disc. In this embodiment, the through hole in the third disc 10 is the third disc through hole II.

[0042] At this point, the air intake channel includes a first air intake channel and a second air intake channel, and the air outlet channel includes a first air outlet channel and a second air outlet channel. The first air intake channel and the first air outlet channel correspond to the mounting position on the right side. The second air intake channel and the second air outlet channel correspond to the mounting position on the left side.

[0043] The first air intake channel includes a first disc through-hole II 82 and a first disc through-hole III 83 through which the gas passes in sequence. The first air outlet channel includes a first disc through-hole IV 84, a second disc groove II 95, a second disc through-hole III 93, and a third disc through-hole I 101 through which the gas passes in sequence.

[0044] The second air intake channel includes a first disc through-hole I81, a second disc through-hole I91, and a second disc groove I94 through which the gas passes in sequence. The second air outlet channel includes a second disc through-hole II92, a second disc groove II95, a second disc through-hole III93, and a third disc through-hole I101 through which the gas passes in sequence.

[0045] To maximize the number of embryos cultured in a single run, this embodiment includes multiple mounting positions on both sides of the embryo culture tray. Specifically, the mounting positions on both sides of the embryo culture tray are evenly distributed circumferentially around the axis of the embryo culture tray. Multiple grooves are included in both the first tray groove I 85 and the second tray groove I 94, and they are radially distributed around the axis of the embryo culture tray.

[0046] As a possible example, in this embodiment, the embryo culture tray further includes a fourth tray 11 located to the left of the third tray 10. The third tray 10 has a third tray groove I 102 on its left side, through which a third tray through hole I 101 passes. The fourth tray 11 has a fourth tray through hole I 111 and a fourth tray through hole II 112. The fourth tray through hole I 111 is directly opposite the third tray groove I 102. The fourth tray through hole II 112 allows the mouth of the culture container 4 on the left to pass through. Multiple third tray grooves I 102 are arranged radially around the axis of the embryo culture tray. In this embodiment, by adding the fourth tray 11, gas can be collected through the third tray groove I 102 between the third tray 10 and the fourth tray 11 and brought to the center of the embryo culture tray for easy collection and outward discharge.

[0047] As a possible example, in this embodiment, the embryo in vitro culture tray further includes a right mounting tray 5 and a left mounting tray 12. The right mounting tray 5 is located on the right side of the first tray body 8, and the left mounting tray 12 is located on the left side of the fourth tray body 11. The right mounting tray 5 has a right mounting hole directly opposite the right mounting position, and the right mounting hole is used to install the right culture container 4. The left mounting tray 12 has a left mounting hole directly opposite the left mounting position, and the left mounting hole is used to install the left culture container 4.

[0048] As a possible example, in this embodiment, both the right-side and left-side mounting holes are threaded holes, meaning the culture container 4 is connected by a threaded connection. The axis of the threaded hole should be parallel to the axis of the embryo culture tray, so that when the culture container 4 is installed on the embryo culture tray, the axis of the culture container 4 is parallel to the axis of the embryo culture tray, thereby reducing the shaking of the culture container 4 when the embryo culture tray rotates around its own axis. By using a threaded connection, it is convenient to disassemble and assemble the culture container 4 when the culture medium inside the culture container 4 needs to be replaced. Depending on the actual needs, the installation method of the culture container 4 on the right-side mounting tray 5 and the left-side mounting tray 12 can be flexibly selected, such as snap-fit ​​or other connection methods.

[0049] As a possible example, to improve airtightness, in this embodiment, the embryo in vitro culture tray further includes an annular sealing plug 6, which includes a right-side sealing plug and a left-side sealing plug. One end of the right-side sealing plug contacts the right side of the first tray 8, and the other end contacts the inner wall of the mouth of the right culture container 4. One end of the left-side sealing plug contacts the left side of the third tray 10, and the other end contacts the inner wall of the mouth of the left culture container 4. In this embodiment, the sealing plug 6 is made of silicone; however, those skilled in the art may choose other materials depending on the actual needs. In this embodiment, the right side of the first tray 8 has a groove for positioning the right-side sealing plug, and the left side of the third tray 10 has a groove for positioning the left-side sealing plug, to improve installation accuracy.

[0050] Reference Figures 1-4 This embodiment also provides an in vitro embryo culture device. The in vitro embryo culture device includes the aforementioned in vitro embryo culture tray, an air inlet pipe 15 located on the right side of the first tray 8, and an air outlet pipe 14 located on the left side of the fourth tray 11. The air inlet pipe 15, the air outlet pipe 14, and the in vitro embryo culture tray are coaxially arranged.

[0051] The left end of the intake pipe 15 has a first outer flange, which is fixedly connected to the first disc body 8. The first outer flange and the first disc body 8 form a first cavity that communicates with the inner hole of the intake pipe 15. The first disc body through hole I 81 and the first disc body through hole II 82 are both connected to the first cavity.

[0052] The right end of the vent pipe 14 has a second outer flange, which is fixedly connected to the fourth disc body 11. The second outer flange and the fourth disc body 11 form a second cavity that communicates with the inner hole of the vent pipe 14. The through hole I111 of the fourth disc body communicates with the first cavity.

[0053] During use, external gas enters the embryo culture dish through the air inlet pipe 15 and leaves the embryo culture dish through the air outlet pipe 14.

[0054] As a possible example, in this embodiment, the embryo in vitro culture tray further includes a fixing support 1 and a pneumatic slip ring. The fixing support 1 includes a right-side fixing support and a left-side fixing support, and the pneumatic slip ring includes a right-side pneumatic slip ring and a left-side pneumatic slip ring.

[0055] The intake pipe 15 is rotatably mounted on the right fixed support, and the right pneumatic slip ring is mounted on the right end of the intake pipe 15. The exhaust pipe 14 is rotatably mounted on the left fixed support, and the left pneumatic slip ring is mounted on the left end of the exhaust pipe 14.

[0056] As a possible example, in this embodiment, the in vitro embryo culture apparatus further includes a drive device, which is connected to the air inlet pipe 15 or the air outlet pipe 14 to drive the in vitro embryo culture tray to rotate via the air inlet pipe 15 or the air outlet pipe 14. Specifically, the drive device includes a drive motor 2, a drive pulley, a driven pulley, and a transmission belt 16. The drive pulley is fixed to the output shaft of the drive motor 2, the driven pulley is fixed to the air inlet pipe 15, and the drive pulley and the driven pulley are connected by the transmission belt 16. Depending on the actual needs, those skilled in the art may also select other types of drive devices.

[0057] As a possible example, in this embodiment, the in vitro embryo culture device further includes a culture container 4, which contains animal embryos and culture medium.

[0058] This specification uses specific examples to illustrate the principles and implementation methods of the present invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of the present invention. Furthermore, those skilled in the art will recognize that, based on the ideas of the present invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. An in vitro embryo culture tray, characterized in that, The embryo in vitro culture tray has a mounting position on at least one side of the tray surface, which is used to connect to the mouth of the culture container; the embryo in vitro culture tray has an air inlet channel and an air outlet channel inside, and the outlet of the air inlet channel and the inlet of the air outlet channel are both located at the mounting position, so that external gas can enter the culture container through the air inlet channel and the gas in the culture container can be discharged to the outside through the air outlet channel. The embryo in vitro culture tray is provided with mounting positions on both the right and left sides; the embryo in vitro culture tray includes a first tray body, a second tray body, and a third tray body arranged sequentially from right to left; The first disc body is provided with a first disc body through hole I, a first disc body through hole II, a first disc body through hole III and a first disc body through hole IV, and a first disc body groove I is provided on the left side of the first disc body; the first disc body groove I connects the first disc body through hole II and the first disc body through hole III; the right end of the first disc body through hole III and the right end of the first disc body through hole IV are directly opposite the mounting position located on the right side; The second disk body is provided with a second disk body through hole I, a second disk body through hole II, and a second disk body through hole III. The left side of the second disk body is provided with a second disk body groove I, and the right side of the second disk body is provided with a second disk body groove II. The second disk body through hole I passes through the second disk body groove I. The second disk body groove II connects the second disk body through hole II and the second disk body through hole III. The first disk body through hole IV is directly opposite the second disk body groove II. The third disk body is provided with a third disk body through hole I, and the third disk body through hole I is directly opposite to the second disk body through hole III; The mounting position on the left side is directly opposite to a portion of the groove I of the second disc and the position of the through hole II of the second disc, through the through hole of the third disc body.

2. The embryo in vitro culture tray according to claim 1, characterized in that, The embryo in vitro culture tray also includes a fourth tray located to the left of the third tray; the third tray has a third tray groove I on its left side, and the third tray through hole I passes through the third tray groove I; the fourth tray has a fourth tray through hole I and a fourth tray through hole II; the fourth tray through hole I is directly opposite the third tray groove I; the fourth tray through hole II allows the mouth of the culture container on the left to pass through.

3. The embryo in vitro culture tray according to claim 2, characterized in that, The embryo in vitro culture tray further includes a right mounting tray and a left mounting tray; the right mounting tray is located on the right side of the first tray body, and the left mounting tray is located on the left side of the fourth tray body; the right mounting tray has a right mounting hole that is directly opposite to the right mounting position, and the right mounting hole is used to install the right culture container; the left mounting tray has a left mounting hole that is directly opposite to the left mounting position, and the left mounting hole is used to install the left culture container.

4. The embryo in vitro culture tray according to claim 3, characterized in that, Both the right-side mounting hole and the left-side mounting hole are threaded holes.

5. The embryo in vitro culture tray according to claim 4, characterized in that, The embryo in vitro culture tray also includes an annular sealing plug, which includes a right sealing plug and a left sealing plug; one end of the right sealing plug contacts the right side of the first tray body, and the other end of the right sealing plug contacts the inner wall of the mouth of the culture container on the right side; one end of the left sealing plug contacts the left side of the third tray body, and the other end of the left sealing plug contacts the inner wall of the mouth of the culture container on the left side.

6. An in vitro embryo culture device, characterized in that, The in vitro embryo culture device includes the in vitro embryo culture tray as described in claim 2, an air inlet pipe located on the right side of the first tray, and an air outlet pipe located on the left side of the fourth tray. The left end of the air intake pipe has a first outer flange, which is fixedly connected to the first disc body. A first cavity is formed between the first outer flange and the first disc body. The first disc body through hole I and the first disc body through hole II are both connected to the first cavity. The right end of the air outlet pipe has a second outer flange, which is fixedly connected to the fourth disc body. A second cavity is formed between the second outer flange and the fourth disc body, and the through hole I of the fourth disc body is connected to the first cavity. The air inlet pipe, the air outlet pipe, and the embryo in vitro culture tray are arranged coaxially.

7. The in vitro embryo culture apparatus according to claim 6, characterized in that, The embryo in vitro culture tray also includes a fixed support and a pneumatic slip ring; the fixed support includes a right fixed support and a left fixed support, and the pneumatic slip ring includes a right pneumatic slip ring and a left pneumatic slip ring; The intake pipe is rotatably mounted on the right fixed support, and the right pneumatic slip ring is mounted on the right end of the intake pipe; the exhaust pipe is rotatably mounted on the left fixed support, and the left pneumatic slip ring is mounted on the left end of the exhaust pipe.

8. The in vitro embryo culture apparatus according to claim 6, characterized in that, The in vitro embryo culture device also includes a driving device, which is connected to the air inlet pipe or the air outlet pipe to drive the in vitro embryo culture tray to rotate through the air inlet pipe or the air outlet pipe.

9. The in vitro embryo culture apparatus according to claim 6, characterized in that, The in vitro embryo culture device also includes the culture container.

Images