Polymerase-reactive catalytic nucleic acid nanostructures

By designing catalytic nucleic acid nanostructures containing DNase/RNase and stimulatory reaction elements, the complexity and cost issues of existing nucleic acid detection technologies have been addressed. This enables rapid, visualized, and modular multiplex nucleic acid and target molecule detection, improving detection speed and sensitivity while reducing false positive results.

CN116018409BActive Publication Date: 2026-07-10NATIONAL UNIVERSITY OF SINGAPORE +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NATIONAL UNIVERSITY OF SINGAPORE
Filing Date
2021-04-07
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing nucleic acid testing technologies face challenges in terms of cost and complexity, making it difficult to achieve rapid, visualized, and modular multiplex nucleic acid and target molecule detection, and are prone to producing false positive results.

Method used

A catalytic nucleic acid nanostructure containing DNase/RNase and a stimulatory response element was designed and developed. It can be used alone in integrated circuits or paired with a target recognition nanostructure. By incorporating G-quadruplex Hemin DNase and polymerase response element, it enables the detection of polymerase activity and reactivity to various stimuli.

Benefits of technology

It improves detection speed and robustness, provides multi-mode readout performance and improved sensitivity, enables robust measurement of molecular targets and their combinations under different environmental conditions, and reduces the occurrence of false positive results.

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Abstract

The present invention relates to catalytic signaling nucleic acid nanostructures responsive to polymerase activity, methods of using the same, devices and kits comprising the same. More specifically, the present invention provides a catalytic signaling nanostructure comprising a DNA / RNA enzyme such as a G-quadruplex hemin and a polymerase responsive element. Polymerase extension of the polymerase responsive element abolishes the catalytic activity of the DNA / RNA enzyme. The catalytic nucleic acid nanostructure can be used alone in an integrated circuit or paired with a target recognition nanostructure that can convert a molecular signal to polymerase activity.
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