Auxiliary device for identifying bamboo-leaf bupleurum by thin layer chromatography

Abstract

This invention discloses an auxiliary device for thin-layer chromatography identification of Bupleurum chinense leaves, relating to the field of traditional Chinese medicine detection technology. It includes a single-slot developing assembly, with a base assembly fixedly mounted at the bottom and a developing solvent containing mechanism nested at the top. A mixing drive mechanism is jointly provided on the top and inside of the developing solvent containing mechanism, and a dual triggering mechanism is provided on the outside of the mixing drive mechanism. This invention reduces the difficulty of preparing the developing solvent for Bupleurum chinense leaf thin-layer chromatography identification, eliminates the need for manual input of the developing solvent, and allows the silica gel thin-layer plate to complete pre-saturation during suspension. Therefore, it eliminates the need for separate slots for placing the developing solvent and silica gel thin-layer plate, saving manpower, reducing the operational difficulty of Bupleurum chinense leaf thin-layer chromatography identification, and improving the efficiency of Bupleurum chinense leaf thin-layer chromatography identification.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine detection technology, and in particular to an auxiliary device for thin-layer identification of bamboo leaves and Bupleurum chinense. Background Technology

[0002] Currently, there are few detection technologies for Bupleurum chinense leaves, especially thin-layer chromatography identification methods, which are even more inadequate. Therefore, the invention patent with authorization announcement number CN 108802259 B discloses a detection method for Bupleurum chinense leaves, which belongs to...

[0003] In the field of traditional Chinese medicine detection technology, this method uses kaerophyllin as a reference and employs thin-layer chromatography for qualitative identification. It has good reproducibility, good separation, simple operation, and low cost, thus enhancing the quality assurance of Bupleurum chinense.

[0004] After preparing the reference solution and the test solution, the above detection method requires taking 5 μL of the reference solution and 5 μL of the test solution respectively, spotting them separately on the same silica gel GF254 thin-layer plate, developing with petroleum ether:acetone:ammonia = 40:20:1 as the developing solvent, removing the plate, drying it, and then examining it under a 254 nm UV lamp.

[0005] However, the above detection method still has some problems in actual use. The most obvious problem is that since the developing solvent is composed of petroleum ether, acetone and ammonia in a specific ratio, technicians need to prepare it manually before testing. After the developing solvent is prepared, it is also necessary to prevent it from falling into the non-developing solvent container in the double-cell developing tank when it is injected into the double-cell developing tank. This is quite difficult to operate and too labor-intensive and time-consuming, which affects the efficiency of thin-layer identification of Bupleurum chinense.

[0006] In addition, the developing tanks currently used for thin-layer chromatography identification of Bupleurum chinense leaves are mostly double-tank setups. In actual testing, one tank is used to hold the developing solvent, and the other tank is used to hold the silica gel GF254 thin-layer plate after the solvent has been applied, so as to facilitate the normal pre-saturation process. After the pre-saturation is completed, the silica gel GF254 thin-layer plate needs to be manually adjusted to move it into the tank containing the developing solvent. The specific adjustment process involves: opening the top cover of the double-tank developing tank, taking out and moving the silica gel GF254 thin-layer plate, and resetting the top cover of the double-tank developing tank. The operation is cumbersome and wastes manpower.

[0007] Therefore, it is necessary to invent an auxiliary device for thin-layer identification of bamboo leaves and Bupleurum chinense to solve the above problems. Summary of the Invention

[0008] The purpose of this invention is to provide an auxiliary device for thin-layer identification of bamboo leaves and Bupleurum chinense, so as to solve the problems mentioned in the background art.

[0009] To achieve the above objectives, the present invention provides the following technical solution: a thin-layer identification auxiliary device for Bupleurum chinense leaves, comprising a single-slot unfolding component, a base component fixedly disposed at the bottom of the single-slot unfolding component, a developing agent containing mechanism fixedly nested at the top of the single-slot unfolding component, a mixing drive mechanism jointly disposed at the top and inside of the developing agent containing mechanism, a dual triggering mechanism disposed on the outside of the mixing drive mechanism, both the mixing drive mechanism and the dual triggering mechanism extending into the interior of the single-slot unfolding component, and a lifting clamping mechanism disposed inside the single-slot unfolding component, the lifting clamping mechanism clamping a silicone thin-layer plate on its front side;

[0010] The single-slot deployment assembly includes a deployment shell, a sealing door, a bar magnet, a deployant recovery pipe, and a shut-off valve;

[0011] The front of the unfolding shell is provided with an opening for inserting and removing the silicone sheet. There are two sealing doors and two bar magnets. The two sealing doors are respectively mounted on the two sides of the front of the unfolding shell by hinges. The two bar magnets are respectively fixedly nested at the edges of the two sealing doors. The unfolding agent recovery tube is fixedly installed through the bottom left side of the unfolding shell. The shut-off valve is installed on the unfolding agent recovery tube.

[0012] The base assembly includes a counterweight base, a placement slot, and a recycling cup;

[0013] The counterweight base is fixedly installed at the bottom of the counterweight base, the placement slot is opened on the left side of the front of the counterweight base, the developing agent recovery tube passes through the top of the counterweight base and extends to the top of the inner side of the placement slot, and the recovery cup is placed inside the placement slot.

[0014] The developing agent containing mechanism includes a containing tank, a liquid inlet hopper, a partition plate, and a liquid guiding channel;

[0015] The container is fixedly nested on the top of the unfolded shell, the liquid inlet is fixedly nested on the left side of the container, the top of the liquid inlet is threaded with a sealing plug, the partition plate is fixedly set in the middle of the inner cavity of the container, and the liquid guiding channel is set through the center of the top of the partition plate.

[0016] Preferably, the mixing drive mechanism includes a hollow drive shaft, a drive motor, a drive gear, a liquid inlet, and a mixing rod.

[0017] Preferably, the hollow drive shaft passes through the top of the container and extends into the interior of the unfolded shell. The hollow drive shaft is rotatably connected to the container via bearings. The drive motor is fixedly installed on the right side of the top of the container. There are two drive gears. The hollow drive shaft and the drive motor are connected by the two drive gears. The liquid inlet is opened on the top front of the hollow drive shaft. There are multiple mixing rods. The multiple mixing rods are evenly fixedly installed on the top of both sides of the hollow drive shaft.

[0018] Preferably, the dual triggering mechanism includes a sealing plate, a telescopic rod, a sliding sleeve, a sliding block, a first spring, a traction rod, and a trigger block.

[0019] Preferably, the sealing plate is slidably sleeved on the outside of the hollow drive shaft and fits against the bottom of the partition plate. The sealing plate blocks the liquid guiding channel. Two telescopic rods, two sliding sleeves, two sliding blocks, two first springs, and two traction rods are provided. The two telescopic rods are respectively fixedly connected to the bottom sides of the sealing plate and are both fixedly connected to the inside of the receiving tank. The two sliding sleeves are respectively fixedly inserted through the bottom sides of the receiving tank and are both fixedly connected to the sealing plate. The two sliding blocks are respectively slidably disposed on the inside of the two sliding sleeves. The two first springs are respectively fixedly connected to the top of the two sliding blocks. The top of the first spring is fixedly connected to the inner wall of the adjacent sliding sleeve. The two traction rods are respectively fixedly disposed at the bottom of the two sliding blocks and slide to the outside of the two sliding sleeves. The trigger block is sleeved on the outside of the hollow drive shaft and is connected to the hollow drive shaft through a reciprocating thread. The bottom ends of the two traction rods are fixedly connected to the trigger block.

[0020] Preferably, the lifting clamping mechanism includes a back plate, a clamping groove, a clamping plate, a guide groove, a receiving groove, a sliding plate, a second spring, a sliding rod, and a trigger rod.

[0021] Preferably, the back plate is slidably sleeved on the outside of the hollow drive shaft and fixedly connected to the trigger block. The clamping groove is opened at the bottom front of the back plate. Two clamping plates, guide grooves, receiving grooves, sliding plates, second springs, sliding rods, and trigger rods are provided. The two clamping plates are slidably disposed at both ends inside the clamping groove. The two guide grooves are respectively opened through the top two sides of the back plate. The two receiving grooves are respectively opened inside the two sides of the back plate. The two sliding plates are respectively slidably disposed inside the two receiving grooves. The two second springs are respectively fixedly connected to the sides of the two sliding plates and are fixedly connected to the inner walls of the adjacent receiving grooves. The two sliding rods are respectively fixedly disposed through the two sliding plates. One end of the sliding rod is fixedly connected to the adjacent clamping plate, and the other end slides to the inner side of the adjacent guide groove. The two trigger rods are respectively slidably disposed inside the two guide grooves. Both ends of the trigger rods are fixedly connected to the inner wall of the unfolded shell.

[0022] This invention also provides a method for using the thin-layer identification auxiliary device for bamboo leaves and Bupleurum chinense, specifically including the following steps:

[0023] S1. Prepare a reference solution from kaerophyllin reference standard, and prepare a test solution from bamboo leaf and Bupleurum powder.

[0024] S2. Take the above reference solution and test solution and spot them on a silica gel thin layer plate respectively. Then add petroleum ether, acetone and ammonia into the inlet according to the preset ratio. After entering the container, petroleum ether, acetone and ammonia are blocked by the partition plate and the hollow drive shaft. At this time, petroleum ether, acetone and ammonia undergo preliminary mixing.

[0025] S3. Open the sealed door and take out the silicone sheet, so that the top of the silicone sheet enters the clamping groove. At this time, start the drive motor. After the drive motor starts, it drives the hollow drive shaft to rotate through two drive gears. When the hollow drive shaft rotates, it drives multiple mixing rods to rotate synchronously, thereby mixing the initially mixed petroleum ether, acetone and ammonia.

[0026] S4. When the hollow drive shaft rotates, it drives the trigger block. After being driven, the trigger block descends along the hollow drive shaft. When the trigger block descends, it pushes the back plate to descend synchronously. When the descending distance of the trigger block reaches the first threshold, the protrusion on the inner side of the trigger rod enters the inner side of the guide groove and pushes the clamping plate through the sliding rod, so that the two clamping plates clamp the clamping groove from both sides respectively.

[0027] S5. At this time, the technician removes his hand from the silicone sheet and closes the two sealing doors. When the trigger block descends, it drives the sliding block to descend synchronously through the traction rod. When the sliding block descends, the first spring that was compressed gradually returns to its original position. When the distance the trigger block descends reaches the second threshold, the first spring is fully reset. Subsequently, as the trigger block continues to descend, it drives the sealing plate to descend through the sliding block, the first spring, and the sliding sleeve. When the sealing plate descends, it compresses the telescopic rod and releases the blockage of the liquid guiding channel. At this time, the developing agent that has been mixed inside the container falls into the bottom of the container cavity through the liquid guiding channel, and then enters the hollow drive shaft through the liquid inlet hole. Then, it flows into the bottom of the developing shell cavity through the bottom opening of the hollow drive shaft.

[0028] S6. When the trigger block descends to the third threshold, the telescopic rod is compressed to its limit by the sealing plate. At this point, the telescopic rod can no longer be compressed. Subsequently, as the trigger block continues to descend, the trigger block stretches the first spring through the traction rod and the sliding block. At this point, all the unfolding agent inside the container enters the unfolding shell, and the silica gel sheet begins to perform pre-saturation operation during the descent.

[0029] S7. When the trigger block descends to the fourth threshold, the pre-saturation of the silicone sheet is completed. As the trigger block continues to descend, the bottom of the silicone sheet is immersed in the spreading agent for spreading. When the trigger block descends to the fifth threshold, the trigger block moves to the lowest end of the reciprocating thread on the outside of the hollow drive shaft. As the hollow drive shaft continues to rotate, the trigger block begins to drive the silicone sheet to rise.

[0030] S8. When the trigger block rises to the sixth threshold, the silicone sheet is fully expanded and removed from the developing agent. At this time, the technician opens the shut-off valve so that the developing agent inside the expanding shell flows into the recovery cup through the developing agent recovery pipe and is recovered. At the same time, the two sealing doors are opened. When the trigger block drives the silicone sheet to reset, the silicone sheet is removed.

[0031] S9. After removing the silica gel thin-layer plate and drying it, place it under a UV lamp at 254nm for inspection. In the chromatogram of the test sample, spots of the same color appear at the corresponding positions as in the chromatogram of the reference sample.

[0032] The technical effects and advantages of this invention are as follows:

[0033] This invention incorporates a developing agent containing mechanism, a mixing drive mechanism, a dual triggering mechanism, and a lifting clamping mechanism. The mixing drive mechanism mixes the developing agent within the developing agent containing mechanism while simultaneously driving the dual triggering mechanism. Upon activation, the dual triggering mechanism first triggers the lifting clamping mechanism, enabling it to automatically clamp the silicone sheet. Subsequently, the dual triggering mechanism triggers the developing agent containing mechanism, releasing the blockage and allowing the mixed developing agent to automatically flow into the single-tank developing assembly. The dual triggering mechanism continuously drives the lifting clamping mechanism, which in turn causes the lifting clamping mechanism to drive the silica gel thin-layer plate to complete the pre-saturation and unfolding operations. Compared with similar devices in the prior art, this invention reduces the difficulty of preparing the developing agent during the thin-layer identification of Bupleurum chinense leaves, and eliminates the need for manual input of the developing agent. In addition, the silica gel thin-layer plate can complete the pre-saturation operation while suspended, so there is no need to set up two grooves to place the developing agent and the silica gel thin-layer plate separately, saving manpower, reducing the difficulty of the thin-layer identification of Bupleurum chinense leaves, and improving the efficiency of the thin-layer identification of Bupleurum chinense leaves. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the overall front view of the present invention.

[0035] Figure 2 This is a schematic diagram of the overall front cross-sectional structure of the present invention.

[0036] Figure 3This is a front cross-sectional view of the developing agent containing mechanism and the mixing driving mechanism of the present invention.

[0037] Figure 4 This is a front cross-sectional view of the dual triggering mechanism of the present invention.

[0038] Figure 5 This is a schematic diagram of the overall front cross-sectional structure of the lifting clamping mechanism of the present invention.

[0039] Figure 6 This is a partial front cross-sectional view of the lifting clamping mechanism of the present invention.

[0040] In the diagram: 1. Single-tank development assembly; 11. Development shell; 12. Sealing door; 13. Bar magnet; 14. Development solvent recovery pipe; 15. Shut-off valve; 2. Base assembly; 21. Counterweight base; 22. Placement tank; 23. Recovery cup; 3. Development solvent receiving mechanism; 31. Receiving tank; 32. Inlet hopper; 33. Divider plate; 34. Liquid guiding channel; 4. Mixing drive mechanism; 41. Hollow drive shaft; 42. Drive motor; 43. Drive gear ; 44. Liquid inlet; 45. Mixing rod; 5. Dual triggering mechanism; 51. Sealing plate; 52. Telescopic rod; 53. Sliding sleeve; 54. Sliding block; 55. First spring; 56. Traction rod; 57. Trigger block; 6. Lifting clamping mechanism; 61. Back plate; 62. Clamping groove; 63. Clamping plate; 64. Guide groove; 65. Receiving groove; 66. Sliding plate; 67. Second spring; 68. Sliding rod; 69. Trigger rod; 7. Silicone film. Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example

[0042] This invention provides, for example Figure 1-6 The illustrated auxiliary device for thin-layer identification of Bupleurum chinense leaves includes a single-slot unfolding component 1. A base component 2 is fixedly installed at the bottom of the single-slot unfolding component 1. A developing agent containing mechanism 3 is fixedly nested at the top of the single-slot unfolding component 1. A mixing drive mechanism 4 is jointly provided at the top and inside of the developing agent containing mechanism 3. A double triggering mechanism 5 is provided on the outside of the mixing drive mechanism 4. Both the mixing drive mechanism 4 and the double triggering mechanism 5 extend into the interior of the single-slot unfolding component 1. A lifting clamping mechanism 6 is provided inside the single-slot unfolding component 1. The lifting clamping mechanism 6 clamps a silicone thin-layer plate 7 on its front side.

[0043] like Figure 2 As shown, the single-slot unfolding assembly 1 includes an unfolding shell 11, a sealing door 12, a bar magnet 13, an unfolding agent recovery pipe 14, and a shut-off valve 15. The unfolding shell 11 has an opening on its front side for inserting and removing the silicone thin film 7. There are two sealing doors 12 and two bar magnets 13. The two sealing doors 12 are respectively hinged and rotated on both sides of the front side of the unfolding shell 11. The two bar magnets 13 are respectively fixedly nested at the edges of the two sealing doors 12. The unfolding agent recovery pipe 14 is fixedly inserted through the bottom left side of the unfolding shell 11. The shut-off valve 15 is disposed on the unfolding agent recovery pipe 14.

[0044] like Figure 2 As shown, the base assembly 2 includes a counterweight base 21, a placement slot 22, and a recycling cup 23. The counterweight base 21 is fixedly disposed at the bottom of the counterweight base 21. The placement slot 22 is opened on the left side of the front of the counterweight base 21. The expander recycling tube 14 passes through the top of the counterweight base 21 and extends to the top of the inner side of the placement slot 22. The recycling cup 23 is placed inside the placement slot 22.

[0045] By setting up the above-mentioned single-slot unfolding assembly 1 and base assembly 2, the shut-off valve 15 can be opened after unfolding is completed. At this time, the unfolding agent at the bottom of the inner cavity of the unfolding shell 11 flows into the recovery cup 23 through the unfolding agent recovery pipe 14 and is recovered.

[0046] like Figure 3 As shown, the developing agent containing mechanism 3 includes a containing tank 31, a liquid inlet 32, a partition plate 33, and a liquid guiding channel 34. The containing tank 31 is fixedly nested on the top of the developing shell 11, the liquid inlet 32 ​​is fixedly nested on the left side of the containing tank 31, and a sealing plug is threaded to the top of the liquid inlet 32. The partition plate 33 is fixedly disposed in the middle of the inner cavity of the containing tank 31, and the liquid guiding channel 34 is disposed through the center of the top of the partition plate 33.

[0047] By setting the above structure, petroleum ether, acetone and ammonia are added into the liquid inlet hopper 32 in a preset ratio. After entering the container tank 31, the petroleum ether, acetone and ammonia are blocked by the partition plate 33. At this time, the petroleum ether, acetone and ammonia undergo preliminary mixing.

[0048] like Figure 3As shown, the mixing drive mechanism 4 includes a hollow drive shaft 41, a drive motor 42, drive gears 43, a liquid inlet 44, and mixing rods 45. The hollow drive shaft 41 passes through the top of the container tank 31 and extends into the interior of the unfolded shell 11. The hollow drive shaft 41 is rotatably connected to the container tank 31 via bearings. The drive motor 42 is fixedly installed on the right side of the top of the container tank 31. There are two drive gears 43, and the hollow drive shaft 41 and the drive motor 42 are connected by transmission through the two drive gears 43. The liquid inlet 44 is opened on the top front of the hollow drive shaft 41. There are multiple mixing rods 45, which are evenly fixedly installed on the top sides of the hollow drive shaft 41.

[0049] By setting the above structure, the drive motor 42 drives the hollow drive shaft 41 to rotate through the drive gear 43. When the hollow drive shaft 41 rotates, the developing agent to be mixed is mixed through the mixing rod 45. At the same time, when the developing agent flows into the bottom of the inner cavity of the container 31, it can enter the hollow drive shaft 41 through the liquid inlet hole 44, and then flow into the bottom of the inner cavity of the developing shell 11 from the bottom opening of the hollow drive shaft 41.

[0050] like Figure 3 and Figure 4 As shown, the dual triggering mechanism 5 includes a sealing plate 51, a telescopic rod 52, a sliding sleeve 53, a sliding block 54, a first spring 55, a traction rod 56, and a trigger block 57. The sealing plate 51 is slidably sleeved on the outside of the hollow drive shaft 41 and fits against the bottom of the partition plate 33, sealing the liquid guiding channel 34. Two telescopic rods 52, two sliding sleeves 53, two sliding blocks 54, two first springs 55, and two traction rods 56 are provided. The two telescopic rods 52 are respectively fixedly connected to the bottom sides of the sealing plate 51 and are both fixedly connected to the inside of the receiving tank 31. The two sliding sleeves 53 are respectively fixedly inserted through the sealing plate. The two sliding blocks 54 are fixedly connected to the bottom of the container 31 and to the sealing plate 51. The two sliding blocks 54 are respectively slidably disposed inside the two sliding sleeves 53. The two first springs 55 are respectively fixedly connected to the top of the two sliding blocks 54. The top of the first spring 55 is fixedly connected to the inner wall of the adjacent sliding sleeve 53. The two traction rods 56 are respectively fixedly disposed at the bottom of the two sliding blocks 54 and respectively slide to the outside of the two sliding sleeves 53. The trigger block 57 is sleeved on the outside of the hollow drive shaft 41 and is connected to the hollow drive shaft 41 through a reciprocating thread. The bottom ends of the two traction rods 56 are fixedly connected to the trigger block 57.

[0051] By setting the above structure, when the trigger block 57 descends, the first compressed spring 55 is first reset by the traction rod 56 and the sliding block 54. After the first spring 55 is fully reset, as the trigger block 57 continues to descend, the trigger block 57 starts to drive the sealing plate 51 to descend through the traction rod 56, the sliding block 54, the first spring 55 and the sliding sleeve 53. When the sealing plate 51 descends, it compresses the telescopic rod 52 and at the same time drives the sealing plate 51 to release the blockage of the liquid guiding channel 34. At this time, the spreading agent inside the container tank 31 can flow through the liquid guiding channel 34 into the bottom of the inner cavity of the container tank 31.

[0052] like Figure 5 and Figure 6 As shown, the lifting clamping mechanism 6 includes a back plate 61, a clamping groove 62, a clamping plate 63, a guide groove 64, a receiving groove 65, a sliding plate 66, a second spring 67, a sliding rod 68, and a trigger rod 69. The back plate 61 is slidably sleeved on the outside of the hollow drive shaft 41 and fixedly connected to the trigger block 57. The clamping groove 62 is formed at the bottom front of the back plate 61. Two clamping plates 63, guide grooves 64, receiving grooves 65, sliding plates 66, second springs 67, sliding rods 68, and trigger rods 69 are each provided. Two clamping plates 63 are slidably disposed at both ends inside the clamping groove 62. Two guide grooves 64 are respectively provided through the back plate 61. On the top two sides, two receiving slots 65 are respectively opened inside the back plate 61. Two sliding plates 66 are respectively slidably disposed inside the two receiving slots 65. Two second springs 67 are respectively fixedly connected to the sides of the two sliding plates 66 and are both fixedly connected to the inner wall of the adjacent receiving slot 65. Two sliding rods 68 are respectively fixedly disposed through the two sliding plates 66. One end of the sliding rod 68 is fixedly connected to the adjacent clamping plate 63 and the other end slides to the inner side of the adjacent guide groove 64. Two trigger rods 69 are respectively slidably disposed inside the two guide grooves 64. Both ends of the trigger rods 69 are fixedly connected to the inner wall of the unfolded shell 11.

[0053] By setting the above structure, the silicone sheet 7 can be easily picked up, so that the top of the silicone sheet 7 enters the inner side of the clamping groove 62. Subsequently, as the back plate 61 continues to descend, the protrusion on the inner side of the trigger rod 69 pushes the sliding rod 68, thereby causing the sliding rod 68 to drive the clamping plate 63 to be clamped by the silicone sheet 7 on the side, thus completing the fixation of the silicone sheet 7. When the back plate 61 is reset, the trigger rod 69 no longer pushes the sliding rod 68. At this time, under the push of the second spring 67, the sliding plate 66 slides outward inside the receiving groove 65, thereby driving the clamping plate 63 to reset through the sliding rod 68, releasing the clamping groove 62. Example

[0054] This invention also provides a method for using the thin-layer identification auxiliary device for bamboo leaves and Bupleurum chinense, specifically including the following steps:

[0055] S1. Prepare a reference solution from kaerophyllin reference standard, and prepare a test solution from bamboo leaf and Bupleurum powder.

[0056] S2. Take the above reference solution and test solution and spot them on the silica gel thin layer plate 7 respectively. Then add petroleum ether, acetone and ammonia into the liquid inlet 32 ​​in a preset ratio. After the petroleum ether, acetone and ammonia enter the container 31, they are blocked by the partition plate 33 and the hollow drive shaft 41. At this time, the petroleum ether, acetone and ammonia are initially mixed.

[0057] S3. Open the sealing door 12 and take out the silicone thin film 7 so that the top of the silicone thin film 7 enters the inner side of the clamping groove 62. At this time, start the drive motor 42. After the drive motor 42 starts, it drives the hollow drive shaft 41 to rotate through two drive gears 43. When the hollow drive shaft 41 rotates, it drives multiple mixing rods 45 to rotate synchronously, thereby mixing the initially mixed petroleum ether, acetone and ammonia.

[0058] S4. When the hollow drive shaft 41 rotates, it drives the trigger block 57. After being driven, the trigger block 57 descends along the hollow drive shaft 41. When the trigger block 57 descends, it pushes the back plate 61 to descend synchronously. When the descending distance of the trigger block 57 reaches the first threshold, the protrusion on the inner side of the trigger rod 69 enters the inner side of the guide groove 64 and pushes the clamping plate 63 through the sliding rod 68, so that the two clamping plates 63 clamp the clamping groove 62 from both sides respectively.

[0059] S5. At this time, the technician removes his hand from the silicone thin plate 7 and closes the two sealing doors 12. When the trigger block 57 descends, it drives the sliding block 54 to descend synchronously through the traction rod 56. When the sliding block 54 descends, the compressed first spring 55 gradually resets. When the descent distance of the trigger block 57 reaches the second threshold, the first spring 55 is completely reset. Subsequently, as the trigger block 57 continues to descend, the trigger block 57 drives the sealing plate 51 to descend through the sliding block 54, the first spring 55 and the sliding sleeve 53. When the sealing plate 51 descends, it compresses the telescopic rod 52 and releases the blockage of the liquid guiding channel 34. At this time, the developing agent mixed inside the container 31 falls into the bottom of the inner cavity of the container 31 through the liquid guiding channel 34, and then enters the hollow drive shaft 41 through the liquid inlet 44. Then, it flows into the bottom of the inner cavity of the developing shell 11 through the bottom opening of the hollow drive shaft 41.

[0060] S6. When the trigger block 57 descends to the third threshold, the telescopic rod 52 is compressed to the limit by the sealing plate 51. At this time, the telescopic rod 52 can no longer be compressed. Subsequently, as the trigger block 57 continues to descend, the trigger block 57 stretches the first spring 55 through the traction rod 56 and the sliding block 54. At this time, all the unfolding agent inside the container 31 enters the unfolding shell 11, and the silicone thin plate 7 begins to perform pre-saturation operation during the descent.

[0061] S7. When the trigger block 57 descends to the fourth threshold, the silicone sheet 7 is pre-saturated. As the trigger block 57 continues to descend, the bottom of the silicone sheet 7 is immersed in the spreading agent for spreading. When the trigger block 57 descends to the fifth threshold, the trigger block 57 moves to the lowest end of the reciprocating thread on the outside of the hollow drive shaft 41. As the hollow drive shaft 41 continues to rotate, the trigger block 57 begins to drive the silicone sheet 7 to rise.

[0062] S8. When the rising distance of the trigger block 57 reaches the sixth threshold, the silicone sheet 7 is fully unfolded and removed from the unfolding agent. At this time, the technician opens the shut-off valve 15 so that the unfolding agent inside the unfolding shell 11 flows into the recovery cup 23 through the unfolding agent recovery pipe 14 and is recovered. At the same time, the two sealing doors 12 are opened. When the trigger block 57 drives the silicone sheet 7 to reset, the silicone sheet 7 is taken out.

[0063] S9. After removing the silica gel thin-layer plate 7 and drying it, place it under a UV lamp at 254nm for inspection. In the chromatogram of the test sample, spots of the same color appear at the corresponding positions as in the chromatogram of the reference sample.

[0064] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A thin-layer chromatography auxiliary device for identifying bamboo leaves and Bupleurum chinense, characterized in that: The assembly includes a single-slot unfolding component (1), a base component (2) is fixedly installed at the bottom of the single-slot unfolding component (1), an unfolding agent containing mechanism (3) is fixedly nested at the top of the single-slot unfolding component (1), a mixing drive mechanism (4) is jointly installed at the top of the unfolding agent containing mechanism (3) and inside the unfolding agent containing mechanism (3), a double triggering mechanism (5) is installed on the outside of the mixing drive mechanism (4), both the mixing drive mechanism (4) and the double triggering mechanism (5) extend into the interior of the single-slot unfolding component (1), a lifting clamping mechanism (6) is installed inside the single-slot unfolding component (1), and the lifting clamping mechanism (6) clamps a silicone thin sheet (7) on the front. The single-slot deployment assembly (1) includes a deployment shell (11), a sealing door (12), a bar magnet (13), a deployant recovery pipe (14), and a shut-off valve (15). The front of the unfolding shell (11) is provided with an opening for inserting and removing the silicone sheet (7). There are two sealing doors (12) and two bar magnets (13). The two sealing doors (12) are respectively mounted on the two sides of the front of the unfolding shell (11) by hinges. The two bar magnets (13) are respectively fixedly nested at the edges of the two sealing doors (12). The unfolding agent recovery pipe (14) is fixedly installed through the bottom left side of the unfolding shell (11). The shut-off valve (15) is installed on the unfolding agent recovery pipe (14). The base assembly (2) includes a counterweight base (21), a placement slot (22), and a recycling cup (23); The counterweight base (21) is fixedly installed at the bottom of the unfolding shell (11), the placement slot (22) is opened on the left side of the front of the counterweight base (21), the unfolding agent recovery pipe (14) passes through the top of the counterweight base (21) and extends to the top of the inner side of the placement slot (22), and the recovery cup (23) is placed inside the placement slot (22). The developing agent containing mechanism (3) includes a containing tank (31), a liquid inlet hopper (32), a partition plate (33), and a liquid guiding channel (34). The container (31) is fixedly nested on the top of the unfolded shell (11), the liquid inlet (32) is fixedly nested on the left side of the container (31), the top of the liquid inlet (32) is threaded with a sealing plug, the partition plate (33) is horizontally fixed in the middle of the inner cavity of the container (31), and the liquid guiding channel (34) is through the center of the partition plate (33). The mixing drive mechanism (4) includes a hollow drive shaft (41), a drive motor (42), a drive gear (43), a liquid inlet (44), and a mixing rod (45). The hollow drive shaft (41) passes through the top of the container (31) and extends into the interior of the unfolded shell (11). The hollow drive shaft (41) is rotatably connected to the container (31) through bearings. The drive motor (42) is fixedly installed on the right side of the top of the container (31). There are two drive gears (43). The hollow drive shaft (41) and the drive motor (42) are connected by transmission through the two drive gears (43). The liquid inlet (44) is opened on the front of the hollow drive shaft (41) and located below the partition plate (33). There are multiple mixing rods (45). The multiple mixing rods (45) are evenly fixed on both sides of the hollow drive shaft (41) and located above the partition plate (33). The dual triggering mechanism (5) includes a closed plate (51), a telescopic rod (52), a sliding sleeve (53), a sliding block (54), a first spring (55), a traction rod (56), and a trigger block (57); The sealing plate (51) is slidably sleeved on the outside of the hollow drive shaft (41) and fits against the bottom of the partition plate (33). The sealing plate (51) seals the liquid guiding channel (34). Two telescopic rods (52), two sliding sleeves (53), two sliding blocks (54), two first springs (55), and two traction rods (56) are provided. The two telescopic rods (52) are respectively fixedly connected to the bottom sides of the sealing plate (51) and are both fixedly connected to the inside of the container (31). The two sliding sleeves (53) are respectively fixedly inserted through the bottom sides of the container (31) and are both fixedly connected to the sealing plate (51). The moving blocks (54) are slidably disposed inside the two sliding sleeves (53), the two first springs (55) are fixedly connected to the top of the two sliding blocks (54), the top of the first springs (55) is fixedly connected to the inner wall of the adjacent sliding sleeves (53), the two traction rods (56) are fixedly disposed at the bottom of the two sliding blocks (54) and slidably extend to the outside of the two sliding sleeves (53), the trigger block (57) is sleeved on the outside of the hollow drive shaft (41) and is connected to the hollow drive shaft (41) through a reciprocating thread, and the bottom ends of the two traction rods (56) are fixedly connected to the trigger block (57); The lifting clamping mechanism (6) includes a back plate (61), which is slidably sleeved on the outside of the hollow drive shaft (41) and fixedly connected to the trigger block (57).

2. The auxiliary device for thin-layer identification of bamboo leaves and Bupleurum chinense according to claim 1, characterized in that: The lifting clamping mechanism (6) also includes a clamping groove (62), a clamping plate (63), a guide groove (64), a receiving groove (65), a sliding plate (66), a second spring (67), a sliding rod (68), and a trigger rod (69). The clamping groove (62) is located at the bottom front of the back plate (61). Two clamping plates (63), guide grooves (64), receiving grooves (65), sliding plates (66), second springs (67), sliding rods (68), and trigger rods (69) are provided. Two clamping plates (63) are slidably disposed at both ends inside the clamping groove (62). Two guide grooves (64) are respectively disposed through the top sides of the back plate (61). Two receiving grooves (65) are respectively located on both sides inside the back plate (61). Two sliding plates (66) are respectively slidably disposed in the two receiving grooves (65). Inside, two second springs (67) are fixedly connected to the sides of two sliding plates (66) and are fixedly connected to the inner wall of the adjacent receiving groove (65). Two sliding rods (68) are fixedly installed through the two sliding plates (66). One end of the sliding rod (68) is fixedly connected to the adjacent clamping plate (63) and the other end slides to the inner side of the adjacent guide groove (64). The inner sides of the two trigger rods (69) are provided with protrusions and are slidably installed in the inner side of the two guide grooves (64). Both ends of the trigger rods (69) are fixedly connected to the inner wall of the unfolded shell (11).

3. The method of using the thin-layer identification auxiliary device for bamboo leaves and Bupleurum chinense according to claim 2, characterized in that, Specifically, the following steps are included: S1. Prepare a reference solution from kaerophyllin reference standard, and prepare a test solution from bamboo leaf and Bupleurum powder. S2. Take the above reference solution and test solution and spot them on the silica gel thin layer plate (7). Then add petroleum ether, acetone and ammonia into the liquid inlet (32) in a preset ratio. After the petroleum ether, acetone and ammonia enter the container (31), they are blocked by the partition plate (33) and the hollow drive shaft (41). At this time, the petroleum ether, acetone and ammonia are initially mixed. S3. Open the sealing door (12) and take out the silicone sheet (7) so that the top of the silicone sheet (7) enters the inside of the clamping groove (62). At this time, start the drive motor (42). After the drive motor (42) starts, it drives the hollow drive shaft (41) to rotate through two drive gears (43). When the hollow drive shaft (41) rotates, it drives multiple mixing rods (45) to rotate synchronously, thereby mixing the initially mixed petroleum ether, acetone and ammonia. S4. When the hollow drive shaft (41) rotates, it drives the trigger block (57). After being driven, the trigger block (57) descends along the hollow drive shaft (41). When the trigger block (57) descends, it pushes the back plate (61) to descend synchronously. When the descending distance of the trigger block (57) reaches the first threshold, the protrusion on the inner side of the trigger rod (69) enters the inner side of the guide groove (64) and pushes the clamping plate (63) through the sliding rod (68), so that the two clamping plates (63) clamp the clamping groove (62) from both sides respectively. S5. At this time, the technician removes his hand from the silicone sheet (7) and closes the two sealing doors (12). When the trigger block (57) descends, it drives the sliding block (54) to descend synchronously through the traction rod (56). When the sliding block (54) descends, the compressed first spring (55) gradually resets. When the trigger block (57) descends a distance to the second threshold, the first spring (55) is completely reset. Subsequently, as the trigger block (57) continues to descend, the trigger block (57) is driven by the sliding block (54) and the first spring (55) to descend synchronously. Spring (55) and sliding sleeve (53) drive the closing plate (51) to descend. When the closing plate (51) descends, it compresses the telescopic rod (52) and releases the blockage of the liquid guiding channel (34). At this time, the developing agent mixed inside the container (31) falls into the bottom of the inner cavity of the container (31) through the liquid guiding channel (34), and then enters the hollow drive shaft (41) through the liquid inlet hole (44). Then it flows into the bottom of the inner cavity of the developing shell (11) through the bottom opening of the hollow drive shaft (41). S6. When the trigger block (57) descends to the third threshold, the telescopic rod (52) is compressed to the limit by the sealing plate (51). At this time, the telescopic rod (52) can no longer be compressed. Subsequently, as the trigger block (57) continues to descend, the trigger block (57) stretches the first spring (55) through the traction rod (56) and the sliding block (54). At this time, all the unfolding agent inside the container (31) enters the unfolding shell (11), and the silicone thin film plate (7) begins to perform pre-saturation operation during the descent. S7. When the trigger block (57) descends to the fourth threshold, the silicone sheet (7) is pre-saturated. As the trigger block (57) continues to descend, the bottom of the silicone sheet (7) is immersed in the spreading agent for spreading. When the trigger block (57) descends to the fifth threshold, the trigger block (57) moves to the lowest end of the reciprocating thread on the outside of the hollow drive shaft (41). As the hollow drive shaft (41) continues to rotate, the trigger block (57) begins to drive the silicone sheet (7) to rise. S8. When the trigger block (57) rises to the sixth threshold, the silicone sheet (7) is fully unfolded and removed from the unfolding agent. At this time, the technician opens the shut-off valve (15) so that the unfolding agent inside the unfolding shell (11) flows into the recovery cup (23) through the unfolding agent recovery pipe (14) and is recovered. At the same time, the two sealing doors (12) are opened. When the trigger block (57) drives the silicone sheet (7) to reset, the silicone sheet (7) is taken out. S9. After the silica gel thin-layer plate (7) is taken out and dried, it is then placed under a UV lamp at 254nm for inspection. In the chromatogram of the test sample, spots of the same color appear at the corresponding positions as in the chromatogram of the reference sample.

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