Blood sample dispensing method and blood sample dispensing device

Abstract

The application discloses a blood sample distribution method and a blood sample distribution device. The blood sample distribution method comprises the following steps: obtaining an initial blood sample by a sampling needle; adding a hemolytic agent into a DIFF reaction pool; distributing the initial blood sample into the DIFF reaction pool by the sampling needle to prepare a DIFF reaction liquid; adding a diluent into a WBC reaction pool and distributing the initial blood sample into the WBC reaction pool by the sampling needle to prepare a second sample liquid; cleaning the inner wall and the outer wall of the sampling needle; sucking the second sample liquid from the WBC reaction pool by the sampling needle; adding the hemolytic agent into the WBC reaction pool to prepare a WBC reaction liquid; adding the diluent into an RBC reaction pool and distributing the second sample liquid into the RBC reaction pool by the sampling needle to prepare an RBC reaction liquid. The blood sample distribution method changes the blood distribution sequence of the initial blood sample, and the blood sample is first distributed into the DIFF reaction pool which has a lower requirement for the quantitative accuracy of the blood sample, so that the blood throwing operation is not needed, the blood sample consumption is reduced, and the consumption of the cleaning reagent is saved.

Description

Technical Field

[0001] This invention relates to the field of blood testing technology, and more particularly to a blood sample dispensing method and a blood sample dispensing device. Background Technology

[0002] To improve testing speed, current blood testing devices typically separate blood into the WBC or protein detection pools first. To prevent the blood from the needle tip from being diluted or contaminated, which could affect the accuracy of the test results, the blood from the needle tip is usually discarded before the remaining blood sample is allocated to the detection pools in a predetermined order. A single testing process generally involves two or more blood discarding processes. This blood separation method leads to an increase in the amount of blood used, and the washing process after discarding the blood increases reagent consumption. Summary of the Invention

[0003] The main objective of this invention is to provide a blood sample dispensing method and a blood sample dispensing device, which aims to solve the problem that existing blood testing devices require too many blood samples, resulting in an increased amount of blood used.

[0004] To achieve the above objectives, the present invention provides a blood sample allocation method, comprising the following steps:

[0005] Initial blood samples were obtained by collecting them using a sampling needle;

[0006] Add hemolysin to the DIFF reaction tank;

[0007] The initial blood sample of the first preset number is distributed into the DIFF reaction chamber through the sampling needle to prepare the DIFF reaction solution;

[0008] Add diluent to the WBC reaction chamber and dispense a second preset number of the initial blood samples into the WBC reaction chamber through the sampling needle to prepare a second sample solution;

[0009] The inner and outer walls of the sampling needle are cleaned;

[0010] The sampling needle is used to draw a third predetermined number of the second sample solution from the WBC reaction chamber;

[0011] A hemolytic agent is added to the WBC reaction tank to prepare a WBC reaction solution;

[0012] Add diluent to the RBC reaction chamber and dispense the third preset number of the second sample solution into the RBC reaction chamber through the sampling needle to prepare the RBC reaction solution.

[0013] Preferably, the step of obtaining the initial blood sample through a sampling needle includes:

[0014] Air is drawn in through the sampling needle to form isolation bubbles;

[0015] The initial blood sample is obtained by drawing blood through the sampling needle.

[0016] Following the step of obtaining the initial blood sample via a sampling needle, the method further includes:

[0017] The outer wall of the sampling needle is cleaned using the swab assembly.

[0018] Preferably, the step of distributing a first preset number of the initial blood samples into the DIFF reaction chamber through the sampling needle to prepare the DIFF reaction solution includes:

[0019] The initial blood sample of the first preset number is distributed into the DIFF reaction tank through the sampling needle, and the first hemolysin is added to the DIFF reaction tank through the pipeline to form a vortex in the DIFF reaction tank to obtain a first sample solution after dissolving the red blood cell membrane. Then, air is pushed into the DIFF reaction tank through a positive pressure source to mix the first sample solution with bubbles. After standing for a first preset time, the first sample solution after dissolving the red blood cell membrane is obtained.

[0020] A second hemolytic agent is added to the first sample solution to terminate the hemolysis process of the first hemolytic agent and prevent overlysis of red blood cells in the DIFF reaction tank.

[0021] Air is pushed into the DIFF reaction tank by a positive pressure source for secondary bubble mixing.

[0022] After the DIFF reaction tank is mixed with bubbles twice, the liquid is allowed to stand for a second preset time to obtain the DIFF reaction solution.

[0023] Preferably, the step of distributing a first preset number of the initial blood samples into the DIFF reaction chamber using the sampling needle to obtain the first sample solution includes:

[0024] The first preset number of initial blood samples are added into the DIFF reaction tank through the sampling needle to obtain the first sample solution;

[0025] After the step of distributing a first preset number of initial blood samples into the DIFF reaction chamber through the sampling needle to obtain the first sample solution, the method further includes the following steps:

[0026] The sampling needle is moved upward, and while the outer wall of the sampling needle is cleaned by the swab assembly, blood is ejected through the sampling needle inside the swab assembly.

[0027] Preferably, the step of adding diluent to the WBC reaction chamber and distributing a second preset number of the initial blood samples into the WBC reaction chamber using the sampling needle to prepare a second sample solution includes:

[0028] A second preset number of initial blood samples are added to the WBC reaction chamber through the sampling needle, while diluent is added to the WBC reaction chamber through the pipeline to form a vortex for mixing in the WBC reaction chamber.

[0029] The sampling needle is moved upward, and the outer wall of the sampling needle is cleaned by the swab assembly;

[0030] Air is pushed into the WBC reaction tank by a positive pressure source to mix the mixture in the WBC reaction tank with bubbles.

[0031] After the bubbles are mixed, the mixture is left to stand for a third preset time to obtain the second sample solution.

[0032] Preferably, the step of cleaning the inner and outer walls of the sampling needle includes:

[0033] The outer wall of the sampling needle is cleaned using a swab assembly;

[0034] 0. The cleaning solution is injected into the syringe of the sampling needle to flush it, and the waste liquid after flushing is discharged to clean the inner wall of the sampling needle.

[0035] Preferably, the step of drawing a third predetermined number of the second sample solution from the WBC reaction chamber using the sampling needle includes:

[0036] Before the sampling needle moves downward to draw the second sample liquid in the WBC reaction cell, it draws a preset volume of air to form an isolation bubble in the needle body.

[0037] Insert the sampling needle into the WBC reaction cell and aspirate the third preset number of the second sample solution;

[0038] The sampling needle is moved upward, and while the outer wall of the sampling needle is cleaned by the swab assembly, blood is ejected through the sampling needle inside the swab assembly.

[0039] 0 Preferably, the step of adding a hemolytic agent to the WBC reaction tank to prepare the WBC reaction solution includes:

[0040] A third hemolytic agent is added to the WBC reaction tank to obtain the initial WBC reaction solution;

[0041] Air is pushed into the WBC reaction tank by a positive pressure source to mix the initial WBC reaction solution.

[0042] 5. Allow the initial WBC reaction solution to stand for a fourth preset time to allow the third hemolytic agent to dissolve the red blood cell membrane, release the hemoglobin inside the red blood cells, and obtain the WBC reaction solution.

[0043] Preferably, the step of adding diluent to the RBC reaction chamber and dispensing the third preset number of the second sample solution into the RBC reaction chamber through the sampling needle to prepare the RBC reaction solution includes:

[0044] 0 Simultaneously add the diluent and the third preset number of the second sample solution to the RBC reaction tank to obtain the third sample solution;

[0045] Air is pushed into the RBC cell by a positive pressure source to mix the bubbles.

[0046] The third sample solution is left to stand for a fourth preset time to obtain the RBC reaction solution.

[0047] Preferably, after the step of adding diluent to the WBC reaction chamber and distributing a second preset number of the initial blood samples into the WBC reaction chamber through the sampling needle to obtain the second sample solution, the method further includes the following step:

[0048] The initial blood sample (a fourth preset number of portions) is added into the protein reaction chamber through the sampling needle, while the first protein reagent is added into the protein reaction chamber through the pipeline to form a vortex and mix the samples to obtain the mixed fourth sample solution.

[0049] Air is pushed into the protein reaction chamber by a positive pressure source to perform bubble mixing.

[0050] Let the mixed fourth sample solution stand for a fifth preset time;

[0051] A second protein reagent is added to the fourth sample solution, and air is pushed into the protein reaction cell containing the second protein reagent using a positive pressure source for secondary bubble mixing.

[0052] After the mixture is thoroughly mixed with air bubbles twice, let it stand for a preset time to obtain the protein reaction solution.

[0053] The present invention also provides a blood sample dispensing device for use in the above-described blood sample dispensing method, the blood sample dispensing device comprising:

[0054] A DIFF reaction tank, used for mixing and preparing to obtain a DIFF reaction solution;

[0055] WBC reaction chamber, the WBC reaction chamber is used to mix and prepare to obtain a second sample solution and WBC reaction solution;

[0056] RBC reaction tank, wherein the RBC reaction tank is used to mix and prepare RBC reaction solution;

[0057] A sampling needle is used to collect an initial blood sample and a second sample solution, and to distribute the initial blood sample into the DIFF reaction chamber and the WBC reaction chamber, and to distribute the second sample solution into the RBC reaction chamber;

[0058] Pipelines for adding reaction solution to the DIFF reaction tank, the WBC reaction tank, and the RBC reaction tank;

[0059] A swab assembly for cleaning the outer wall of the sampling needle;

[0060] The control system is used to control the sampling needle to perform blood separation, control the tubing to add reaction solution, and control the swab assembly to perform cleaning.

[0061] In the technical solution of this invention, the quantitative accuracy requirement for DIFF detection is relatively lower than that for WBC detection. After the sampling needle draws blood to be tested to obtain the initial blood sample, it is directly dispensed into the DIFF detection chamber to obtain the DIFF reaction solution, without the need for discarding the first segment of blood. Then, the remaining initial blood sample is allocated to the WBC reaction chamber for dilution to obtain the second sample solution. At this point, the sampling needle is cleaned to wash away the initial blood sample, and the second sample solution is drawn again and allocated to the RBC reaction chamber. Then, the WBC and RBC reaction solutions are mixed in the WBC and RBC reaction chambers to obtain the WBC reaction solution and the RBC reaction solution. This invention changes the order of initial blood sample dispensing, first dispensing blood into the DIFF reaction chamber where the quantitative accuracy requirement is lower, thus eliminating the need for discarding blood, reducing the amount of blood used, and saving the consumption of cleaning reagents. Attached Figure Description

[0062] 0 To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0063] Figure 1 This is a flowchart of a blood sample allocation method according to an embodiment of the present invention;

[0064] Figure 2 This is a detailed flowchart of step S100 of a blood sample allocation method according to an embodiment of the present invention;

[0065] Figure 3 This is a detailed flowchart of step S300 of a blood sample allocation method according to an embodiment of the present invention;

[0066] Figure 4 This is a detailed flowchart of step S400 of a blood sample allocation method according to an embodiment of the present invention;

[0067] Figure 5 This is a detailed flowchart of step S500 of a blood sample allocation method according to an embodiment of the present invention;

[0068] Figure 6 This is a detailed flowchart of step S600 of a blood sample allocation method according to an embodiment of the present invention;

[0069] Figure 7 This is a detailed flowchart of step S700 of a blood sample allocation method according to an embodiment of the present invention;

[0070] Figure 8 This is a detailed flowchart of step S800 of a blood sample allocation method according to an embodiment of the present invention;

[0071] Figure 9 This is a structural block diagram of a blood sample dispensing device according to an embodiment of the present invention.

[0072] 5. The objectives, functional features, and advantages of this invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0073] The technical solutions of this embodiment will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0074] It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in this embodiment are only used to explain the relative positional relationship and movement of each component in a specific posture (as shown in the attached figure). If the specific posture changes, the directional indicator will also change accordingly.

[0075] Furthermore, in this invention, descriptions involving "first," "second," etc., are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0076] In this invention, unless otherwise explicitly specified and limited, the terms "connection," "fixed," etc., should be interpreted broadly. For example, "fixed" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0077] Furthermore, the technical solutions of the various embodiments of the present invention can be combined with each other, but only if they are feasible to those skilled in the art. If a combination of technical solutions contradicts each other or cannot be implemented, such a combination should be considered non-existent and not within the scope of protection claimed by the present invention. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0078] This invention proposes a blood sample allocation method and a blood sample allocation device.

[0079] Please participate Figure 1 The blood sample allocation method in this embodiment includes the following steps:

[0080] S100: Initial blood sample is obtained by collecting a sampling needle;

[0081] S200: Add hemolysin to the DIFF reaction tank;

[0082] S300: The first preset number of initial blood samples are distributed to the DIFF reaction chamber through the sampling needle to prepare the DIFF reaction solution;

[0083] S400: Add diluent to the WBC reaction chamber and use a sampling needle to dispense the second preset number of initial blood samples into the WBC reaction chamber to prepare the second sample solution;

[0084] S500: Clean the inner and outer walls of the sampling needle;

[0085] S600: Aspirate a third preset number of the second sample solution from the WBC reaction chamber using a sampling needle;

[0086] S700: Add hemolysin to the WBC reaction tank to prepare the WBC reaction solution;

[0087] S800: Add diluent to the RBC reaction chamber and dispense the third preset number of the second sample solution into the RBC reaction chamber through the sampling needle to prepare the RBC reaction solution.

[0088] Understandably, the DIFF reaction chamber is used to detect white blood cell differential counts, namely neutrophils, lymphocytes, monocytes, and eosinophils; the WBC (white blood cell) chamber is used for basophils, total white blood cell count, and HGB count; and the RBC (red blood cell) chamber is used to detect RBC and PLT counts. The first, second, and third preset number of samples can be adjusted appropriately according to actual conditions and are not specifically limited.

[0089] In the technical solution of this invention, the quantitative accuracy requirement for DIFF detection is relatively lower than that for WBC detection. After the sampling needle draws blood to be tested to obtain the initial blood sample, it is directly dispensed into the DIFF detection chamber to obtain the DIFF reaction solution, without the need for discarding the first segment of blood. Then, the remaining initial blood sample is allocated to the WBC reaction chamber for dilution to obtain the second sample solution. At this point, the sampling needle is cleaned to wash away the initial blood sample, and the second sample solution is drawn again and allocated to the RBC reaction chamber. Then, the WBC and RBC reaction solutions are mixed in the WBC and RBC reaction chambers to obtain the WBC and RBC reaction solutions. This invention changes the order of initial blood sample dispensing, first dispensing blood into the DIFF reaction chamber where the quantitative accuracy requirement is lower, thus eliminating the need for discarding blood, reducing the amount of blood used, and saving the consumption of cleaning reagents.

[0090] Please see Figure 2 In one embodiment, step S100 includes:

[0091] S110: Air is drawn in through the sampling needle to form isolation bubbles;

[0092] S120: Obtain the initial blood sample by drawing up the blood sample to be tested through the sampling needle;

[0093] After step S100, the following is also included:

[0094] S130: The outer wall of the sampling needle is cleaned using the swab assembly.

[0095] Air is drawn into the sampling needle to form an isolation bubble, and the inside of the transmission tube is filled with diluent. The isolation bubble prevents the initial blood sample from being diluted, ensuring the initial blood sample concentration and improving detection accuracy. At the same time, after collecting the initial blood sample, the outer wall of the sampling needle is cleaned to prevent contaminants on the outer wall of the sampling needle from contaminating the subsequent DIFF reaction cell and WBC reaction cell.

[0096] Please see Figure 3 In one embodiment, step S300 includes:

[0097] S310: The initial blood sample of the first preset number is distributed to the DIFF reaction pool through the sampling needle, and the first hemolysing agent is added to the DIFF reaction pool through the pipeline to form a vortex in the DIFF reaction pool to obtain the first sample solution after dissolving the red blood cell membrane. Then, air is pushed into the DIFF reaction pool through a positive pressure source to mix the first sample solution with bubbles. Then, the sample is left to stand for a first preset time to obtain the first sample solution after dissolving the red blood cell membrane.

[0098] Understandably, the mixture of the first hemolysing agent and the blood sample in the DIFF reaction tank is left to stand for a first preset time so that the first hemolysing agent can change the morphology of white blood cells, slightly damage the red blood cell membrane, and change the volume of white blood cells to obtain the first sample solution.

[0099] S320: Add a second hemolytic agent to the first sample solution to terminate the hemolysis process of the first hemolytic agent and prevent overlysis of red blood cells in the DIFF reaction chamber;

[0100] Understandably, adding a second hemolysin to the first sample solution terminates the effect of the first hemolysin and maintains the morphology and volume of white blood cells;

[0101] S330: Air is pushed into the DIFF reaction tank through a positive pressure source for secondary bubble mixing;

[0102] S340: After the secondary bubble mixing in the DIFF reaction tank is completed, the liquid is allowed to stand for a second preset time to obtain the DIFF reaction solution.

[0103] The initial blood sample and the first hemolysing agent are mixed sequentially with air bubbles, and then incubated to form the first sample. The second hemolysing agent is then added, mixed again with air bubbles, and incubated again to complete the preparation of the DIFF reaction solution. This ensures a uniform distribution of white blood cells in the DIFF reaction solution, making them easier to classify and count. It should be noted that the first and second preset times can be adjusted according to the added hemolysing agent and actual needs, and there are no specific limitations.

[0104] In one embodiment, step S310 includes:

[0105] S3101: Add the first preset number of initial blood samples into the DIFF reaction chamber through the sampling needle to obtain the first sample solution;

[0106] Following step S310, the following steps are also included:

[0107] S3102: Move the sampling needle upwards, and while cleaning the outer wall of the sampling needle through the swab assembly, discard blood through the sampling needle inside the swab assembly.

[0108] While the outer wall of the sampling needle is cleaned by the swab assembly, blood is ejected through the sampling needle inside the swab assembly to prevent the first segment of blood from being contaminated by the first hemolytic agent.

[0109] The sampling needle is lowered into the DIFF reaction chamber for blood separation. The sampling needle is inserted into the DIFF reaction chamber, and the needle tip is immersed below the surface of the first hemolysin solution in the DIFF chamber for blood separation. After blood separation is completed, the outer wall of the sampling needle is cleaned through the swab assembly and the blood is discarded to prevent the blood at the tip of the sampling needle from being contaminated or diluted. This prevents contamination of the WBC reaction chamber when separating blood for the WBC reaction chamber later, thereby improving the detection accuracy of the WBC reaction chamber.

[0110] Please see Figure 4 In one embodiment, step S400 includes:

[0111] S410: Add a second preset number of initial blood samples into the WBC reaction chamber through the sampling needle, and at the same time add diluent into the WBC reaction chamber through the tubing to form a vortex for mixing in the WBC reaction chamber.

[0112] S420: Move the sampling needle upward and clean the outer wall of the sampling needle through the swab assembly;

[0113] S430: Air is pushed into the WBC reaction tank by a positive pressure source to mix the mixture in the WBC reaction tank with bubbles;

[0114] S440: After the mixture is fully mixed with bubbles, let it stand for a third preset time to obtain the second sample solution;

[0115] The sampling needle is lowered into the WBC reaction chamber for blood separation. The needle is inserted into the chamber, and the diluent enters from the side opening at the bottom. The needle tip is submerged in the diluent within the WBC chamber for blood separation. After separation, the outer wall of the sampling needle is cleaned using the swab assembly. Simultaneously, the mixture in the WBC chamber is mixed with air bubbles and incubated to ensure uniform sample distribution and easier counting. It should be noted that the third preset time can be adjusted based on the amount of diluent added and actual needs; there is no specific limitation.

[0116] Please see Figure 5 In one embodiment, step S500 includes:

[0117] S510: The outer wall of the sampling needle is cleaned using the swab assembly;

[0118] S520: The cleaning solution is injected into the syringe of the sampling needle to flush it, and the waste liquid after flushing is discharged to clean the inner wall of the sampling needle.

[0119] The inner and outer walls of the sampling needle are thoroughly cleaned before entering the WBC reaction chamber to draw the second sample solution. This prevents the outer wall of the sampling needle from contaminating the WBC reaction chamber and also prevents the inner wall of the sampling needle from contaminating the second sample solution drawn into the sampling needle, thereby improving the detection accuracy of the subsequent WBC and RBC reaction chambers.

[0120] Please see Figure 6 In one embodiment, step S600 includes:

[0121] S610: Controls the sampling needle to draw in a preset volume of air before it moves downward to draw in the second sample liquid in the WBC reaction cell, so as to form an isolation bubble in the needle body;

[0122] S620: Insert the sampling needle into the WBC reaction cell and aspirate the third preset number of the second sample solution;

[0123] S630: Move the sampling needle upwards, and while cleaning the outer wall of the sampling needle through the swab assembly, discard blood through the sampling needle inside the swab assembly.

[0124] While the outer wall of the sampling needle is cleaned by the swab assembly, blood is ejected through the sampling needle inside the swab assembly to prevent the second sample solution in the tip of the needle from being diluted.

[0125] Before the sampling needle moves downward to draw the second sample solution from the WBC cell, it draws in a preset volume of air to form an isolation bubble inside the needle. This isolation bubble prevents the second sample solution from being diluted, ensuring the concentration of the second sample solution and improving detection accuracy. Then, the needle is inserted into the WBC reaction cell to draw the second sample solution.

[0126] Please see Figure 7 In one embodiment, step S700 includes:

[0127] S710: Add a third hemolysin to the WBC reaction tank to obtain the initial WBC reaction solution;

[0128] S720: Air is pushed into the WBC reaction tank through a positive pressure source to mix the initial WBC reaction solution;

[0129] S730: The initial WBC reaction solution is allowed to stand for a fourth preset time to allow the third hemolysing agent to dissolve the red blood cell membrane, eliminate the influence of red blood cell fragments on the white blood cell count, and combine with the hemoglobin released after the red blood cells rupture to form a stable compound, thus obtaining the WBC reaction solution.

[0130] The initial WBC reaction solution is left to stand for a fourth preset time to allow the third hemolytic agent to dissolve the red blood cell membrane, eliminate the influence of red blood cell fragments on the white blood cell technology, and combine with the hemoglobin released after the red blood cells rupture to form a stable compound, thus obtaining the WBC reaction solution.

[0131] A hemolytic agent was added to the second sample solution to obtain the initial WBC reaction solution. The initial WBC reaction solution was then mixed by introducing air bubbles and incubated to obtain the final WBC reaction solution. This process ensured that the white blood cells in the WBC reaction solution were evenly distributed and easy to count.

[0132] Please see Figure 8 In one embodiment, step S800 includes:

[0133] S810: Simultaneously add diluent and a third preset number of the second sample solution into the RBC reaction chamber to obtain the third sample solution;

[0134] S820: Air is pushed into the RBC cell by a positive pressure source to mix the bubbles;

[0135] S830: Let the third sample solution stand for a fourth preset time to obtain the RBC reaction solution.

[0136] The second sample solution is diluted and mixed again. Due to the high number of red blood cells in the blood, it needs to be diluted again to obtain the RBC reaction solution, which makes the RBC reaction solution more accurate in detecting the number of red blood cells and PLTs. It should be noted that the fourth preset time can be adjusted according to the added diluent and actual needs, and there is no specific limitation.

[0137] In one embodiment, after step S400, the following step is further included:

[0138] S401: Add the fourth preset number of initial blood samples into the protein reaction chamber through the sampling needle, and at the same time add the first protein reagent into the protein reaction chamber through the pipeline to form a vortex in the protein reaction chamber to mix and obtain the mixed fourth sample solution.

[0139] S402: Air is pushed into the protein reaction chamber by a positive pressure source to perform a bubble mixing process;

[0140] S403: Let the mixed fourth sample solution stand for the fifth preset time;

[0141] S404: Add the second protein reagent to the fourth sample solution, and use a positive pressure source to push air into the protein reaction chamber containing the second protein reagent for secondary bubble mixing;

[0142] S405: After the mixture has been mixed with air bubbles twice, let it stand for a preset time to obtain the protein reaction solution.

[0143] Before cleaning the sampling needle, the initial blood sample inside the needle is allocated to the protein pools. Optionally, there can be multiple protein pools to detect C-reactive protein or serum amyloid A in the blood, increasing the variety of blood samples that can be tested. It should be noted that the fifth preset time...

[0144] The sixth preset time can be adjusted according to the added protein reagents and actual needs, and there is no specific limitation. (See also...) Figure 9 The present invention also provides a blood sample dispensing device, applied to the above-mentioned blood sample dispensing method.

[0145] The blood sample dispensing device includes a DIFF reaction chamber, a WBC reaction chamber, an RBC reaction chamber, a sampling needle, tubing, a swab assembly, and a control system. The DIFF reaction chamber is used to mix and prepare the DIFF reaction solution; the WBC reaction chamber is used to mix and prepare the second sample solution and the WBC reaction solution; the RBC reaction chamber...

[0146] The pool is used to mix and prepare the RBC reaction solution; the sampling needle is used to collect the initial blood sample and the second sample solution, and distributes the initial blood sample to the DIFF reaction pool and the WBC reaction pool, and distributes the second sample solution to the RBC reaction pool; the tubing is used to add hemolysing agent and diluent to the DIFF reaction pool, the WBC reaction pool and the RBC reaction pool; the swab assembly is used to clean the inner and outer walls of the sampling needle; the control system is used to control the sampling needle to perform blood dispensing operation, control the tubing to perform the addition of hemolysing agent and diluent operation, and control the swab assembly to perform the cleaning operation.

[0147] Since this blood sample dispensing device adopts all the technical solutions of all the above embodiments, it has at least all the beneficial effects brought about by the technical solutions of the above embodiments, which will not be repeated here.

[0148] The above are merely preferred embodiments of the present invention and do not limit the scope of the patent. Any equivalent structural or procedural transformations made based on the description and drawings of the present invention, or direct or indirect applications in other related technical fields, are similarly included within the scope of patent protection of the present invention.

Claims

1. A method of distributing blood samples, characterized by, The method comprises the following steps: obtaining an initial blood sample through a sampling needle; adding a hemolytic agent into a DIFF reaction pool; dispensing a first preset amount of the initial blood sample into the DIFF reaction pool through the sampling needle to prepare a DIFF reaction solution; adding a diluent into a WBC reaction pool and dispensing a second preset amount of the initial blood sample into the WBC reaction pool through the sampling needle to prepare a second sample solution; cleaning the inner wall and the outer wall of the sampling needle; sucking a third preset amount of the second sample solution from the WBC reaction pool through the sampling needle; adding a hemolytic agent into the WBC reaction pool to prepare a WBC reaction solution; adding a diluent into an RBC reaction pool and dispensing the third preset amount of the second sample solution into the RBC reaction pool through the sampling needle to prepare an RBC reaction solution.

2. The blood sample dispensing method of claim 1, wherein, The step of obtaining an initial blood sample through a sampling needle comprises: sucking air through the sampling needle to form an isolation bubble; sucking a blood sample to be tested through the sampling needle to obtain the initial blood sample; After the step of obtaining an initial blood sample through a sampling needle, the method further comprises: cleaning the outer wall of the sampling needle through a swab assembly.

3. The blood sample dispensing method of claim 1, wherein, The step of dispensing a first preset amount of the initial blood sample into the DIFF reaction pool through the sampling needle to prepare a DIFF reaction solution comprises: dispensing a first preset amount of the initial blood sample into the DIFF reaction pool through the sampling needle, adding a first hemolytic agent into the DIFF reaction pool through a pipeline to form a vortex in the DIFF reaction pool, mixing and dissolving the red blood cell membrane to obtain a first sample solution, then pushing air into the DIFF reaction pool through a positive pressure source to mix the first sample solution with air bubbles, and then standing for a first preset time to obtain the first sample solution after the red blood cell membrane is dissolved; adding a second hemolytic agent to the first sample solution to terminate the hemolysis process of the first hemolytic agent to prevent the red blood cells in the DIFF reaction pool from being over-dissolved; pushing air into the DIFF reaction pool through a positive pressure source for secondary air bubble mixing; standing for a second preset time after the liquid in the DIFF reaction pool is completely mixed with air bubbles to obtain the DIFF reaction solution.

4. The blood sample dispensing method of claim 3, wherein, The step of dispensing a first preset amount of the initial blood sample into the DIFF reaction pool through the sampling needle to obtain a first sample solution comprises: adding the first preset amount of the initial blood sample into the DIFF reaction pool through the sampling needle to obtain the first sample solution. After the step of dispensing a first preset amount of the initial blood sample into the DIFF reaction pool through the sampling needle to obtain a first sample solution, the method further comprises the following steps: moving the sampling needle upward, cleaning the outer wall of the sampling needle through a swab assembly, and throwing blood in the swab assembly through the sampling needle.

5. The blood sample dispensing method of claim 1 wherein, The step of adding a diluent into a WBC reaction pool and dispensing a second preset amount of the initial blood sample into the WBC reaction pool through the sampling needle to prepare a second sample solution comprises: adding a second preset amount of the initial blood sample into the WBC reaction pool through the sampling needle, and adding a diluent into the WBC reaction pool through a pipeline to form a vortex mixing in the WBC reaction pool; moving the sampling needle upward to clean the outer wall of the sampling needle through the swab assembly; pushing air into the WBC reaction pool through a positive pressure source to perform bubble mixing on the mixed liquid in the WBC reaction pool; placing the mixed liquid after the bubble mixing for a third preset time to obtain the second sample liquid.

6. A blood sample dispensing method according to any one of claims 1 to 5, wherein, The step of cleaning the inner wall and the outer wall of the sampling needle comprises: cleaning the outer wall of the sampling needle through the swab assembly; injecting a cleaning liquid into the needle tube of the sampling needle through a syringe to flush, and discharging the waste liquid after the flushing to clean the inner wall of the sampling needle.

7. A blood sample dispensing method according to any one of claims 1 to 5, wherein, The step of drawing the third preset amount of the second sample liquid from the WBC reaction pool through the sampling needle comprises: controlling the sampling needle to suck a preset volume of air before moving downward to draw the second sample liquid in the WBC reaction pool to form an isolation bubble in the needle body; extending the sampling needle into the WBC reaction pool and drawing the third preset amount of the second sample liquid; moving the sampling needle upward to clean the outer wall of the sampling needle through the swab assembly while throwing blood in the swab assembly through the sampling needle.

8. The blood sample dispensing method according to any one of claims 1 to 5, wherein, The step of adding a hemolytic agent into the WBC reaction pool to prepare a WBC reaction liquid comprises: adding a third hemolytic agent into the WBC reaction pool to obtain an initial WBC reaction liquid; pushing air into the WBC reaction pool through a positive pressure source to mix the initial WBC reaction liquid; placing the initial WBC reaction liquid for a fourth preset time to make the third hemolytic agent dissolve the red blood cell membrane, release hemoglobin in the red blood cell, and obtain the WBC reaction liquid.

9. The blood sample dispensing method according to any one of claims 1 to 5, wherein, The step of adding a diluent into the RBC reaction pool and dispensing the third preset amount of the second sample liquid into the RBC reaction pool through the sampling needle to prepare an RBC reaction liquid comprises: simultaneously adding the diluent and the third preset amount of the second sample liquid into the RBC reaction pool to obtain a third sample liquid; pushing air into the RBC reaction pool through a positive pressure source to perform bubble mixing; placing the third sample liquid for a fourth preset time to obtain the RBC reaction liquid.

10. The blood sample dispensing method of any one of claims 1-5, wherein, After the step of adding a diluent into the WBC reaction pool and dispensing the second preset amount of the initial blood sample into the WBC reaction pool through the sampling needle to prepare a second sample liquid, the method further comprises the following steps: adding a fourth preset amount of the initial blood sample into the protein reaction pool through the sampling needle, and adding a first protein reagent into the protein reaction pool through a pipeline to form a vortex mixing in the protein reaction pool to obtain a mixed fourth sample liquid; pushing air into the protein reaction pool through a positive pressure source to perform bubble mixing once; placing the mixed fourth sample liquid for a fifth preset time; A second protein reagent is added to the fourth sample liquid, and air is pushed into the protein reaction pool added with the second protein reagent by a positive pressure source for secondary bubble mixing; The mixed liquid after the secondary bubble mixing is rested for a sixth preset time to obtain a protein reaction liquid.

11. A blood sample dispensing device, characterized by The blood sample distribution device is applied to the blood sample distribution method in any one of claims 1-10, and the blood sample distribution device comprises: a DIFF reaction pool for mixing and preparing a DIFF reaction liquid; a WBC reaction pool for mixing and preparing a second sample liquid and a WBC reaction liquid; an RBC reaction pool for mixing and preparing an RBC reaction liquid; a sampling needle for collecting an initial blood sample and the second sample liquid, and distributing the initial blood sample to the DIFF reaction pool and the WBC reaction pool, and distributing the second sample liquid to the RBC reaction pool; a pipeline for adding a hemolytic agent or a diluent to the DIFF reaction pool, the WBC reaction pool and the RBC reaction pool; a swab assembly for cleaning the outer wall of the sampling needle; a control system for controlling the sampling needle to perform blood distribution, controlling the pipeline to perform the adding of the hemolytic agent or the diluent, and controlling the swab assembly to perform the cleaning.

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