A granular medicine fertilizer for reducing resistance to metalaxyl-M and azoxystrobin and a preparation method thereof

Abstract

The present invention relates to a granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin and a preparation method thereof, including a pharmaceutical component, a fertilizer component, a binder and a sustained-release agent, wherein the pharmaceutical component is 0.05-0.3%, the binder is 1-10%, the sustained-release agent is 1-10%, and the remainder is a fertilizer component; the pharmaceutical component is measured by weight percentage, including 1-20% metalaxyl-M, 1-20% azoxystrobin, 0.01-0.02mg / kg of dactinomycin, 1-3% of Eupatorium adenophorum extract, 2-40% of cyclodextrin, and the remainder is an inert carrier. The granular fertilizer of the present invention can effectively reduce the usage of metalaxyl-M and azoxystrobin, and effectively reduce the generation of drug resistance; at the same time, a trace amount of dactinomycin can affect the RNA synthesis of plant pathogens, thereby reducing the generation of drug resistance. The above structure can effectively reduce the resistance of potato late blight to metalaxyl-M and azoxystrobin complex, and improve the prevention and control effect.

Description

Technical Field

[0001] The invention relates to a granular fertilizer capable of reducing resistance to metalaxyl-M and azoxystrobin and a preparation method thereof, belonging to the field of pesticides. Background Art

[0002] Metalaxyl-M belongs to the phenylamide fungicide class and has the following advantages: (1) Strong systemicity. When sprayed on crops, the fungicide can be absorbed by the seeds, roots, stems, and leaves of the crops and transferred to various parts, which can effectively prevent and control crops scientifically; (2) Metalaxyl-M is an upgraded version of metalaxyl. Under the same conditions, only half of the amount of metalaxyl is needed, which increases safety for the environment and users. It is absorbed into the crop body in a short time, is resistant to rain erosion, and basically does not cause pesticide damage; (3) It has a wide range of application crops: Metalaxyl-M has a good control effect on diseases caused by downy mildew, phytophthora, and pythium, and can be applied to many crops. Metalaxyl-M has been used to control potato late blight for nearly 20 years at home and abroad. Due to the single site of action and the irrational use of the fungicide, it is now facing serious resistance problems. The field performance and control effect have been significantly reduced, and it can no longer effectively control potato late blight. To address metalaxyl-M resistance, a metalaxyl-M combination is typically used. A commonly used combination of metalaxyl-M and azoxystrobin effectively improves prevention effectiveness and reduces resistance. However, over time, potato late blight in the field has developed resistance to both metalaxyl-M and azoxystrobin, resulting in a decrease in control effectiveness. Alternating the use of these agents is necessary to reduce resistance, but the effect remains limited. Summary of the Invention

[0003] The invention provides a granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin and a preparation method thereof, which effectively solves the problem that potato late blight develops resistance to metalaxyl-M and azoxystrobin complex and has poor control effect.

[0004] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0005] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, characterized by comprising a drug component, a fertilizer component, a binder and a sustained-release agent, wherein, by weight, the drug component comprises 0.05-0.3%, the binder comprises 1-10%, the sustained-release agent comprises 1-10%, and the balance is the fertilizer component; and the drug component comprises, by weight, 1-20% of metalaxyl-M, 1-20% of azoxystrobin, 0.01-0.02 mg / kg of dactinomycin, 1-3% of Eupatorium adenophorum extract, 2-40% of cyclodextrin, and the balance is an inert carrier.

[0006] Preferably, the medicinal component further comprises 2-5% Bacillus subtilis powder.

[0007] Preferably, the binder is one or more of xanthan gum, polyvinyl pyrrolidone, sodium carboxymethyl cellulose, cross-linked sodium carboxymethyl cellulose and chitosan.

[0008] Preferably, the sustained-release agent is selected from one or more of polyvinyl alcohol, polyethylene, polypropylene glycol ester and epoxy resin.

[0009] Preferably, the fertilizer is selected from one or more of nitrogen fertilizer, phosphorus fertilizer and potash fertilizer.

[0010] Preferably, the inert carrier includes one or more of kaolin, white carbon black, bentonite, vermiculite, diatomaceous earth, attapulgite, zeolite, and sepiolite.

[0011] The present invention also provides a method for preparing a granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising the following steps:

[0012] (1) 50% of metalaxyl-M and dactinomycin are mixed uniformly for later use, and then a sample is taken at 25-35° C. according to the amount of the mixture of cyclodextrin and metalaxyl-M and dactinomycin being 1:1. The mixture of metalaxyl-M and dactinomycin is slowly added to a saturated aqueous solution of cyclodextrin under continuous stirring, and stirred at 50-60° C. for more than 5 hours to ensure that the inclusion reaction is complete, thereby obtaining a mixture;

[0013] (2) uniformly mixing the mixture prepared in step 1 with the remaining metalaxyl-M, azoxystrobin and Eupatorium adenophorum extract, then adding an inert carrier and mixing uniformly to obtain a pharmaceutical composition;

[0014] (3) uniformly mixing the obtained drug component, the crushed fertilizer component, and the binder, and granulating;

[0015] (4) The granulated product is coated with a sustained-release agent.

[0016] Beneficial effects of the present invention:

[0017] In the present invention, by adding a trace amount of actinomycin and Eupatorium adenophorum extract, the molecule of actinomycin contains a phenoxy ring structure, through which two equivalent cyclic peptide chains are connected. This peptide chain can specifically interact with deoxyguanine of the DNA molecule, enabling dactinomycin D to embed into the minor groove of the DNA double helix, form a complex with DNA, hinder the function of RNA polymerase, and inhibit the synthesis of RNA, especially the synthesis of mRNA. The Eupatorium adenophorum extract is compounded with chemical pesticides, which can not only reduce the dosage of chemical pesticides, reduce residual pollution, but also play a synergistic effect. By adopting the above combination, the usage amounts of metalaxyl-M and azoxystrobin can be effectively reduced, and the generation of drug resistance can be effectively reduced; at the same time, the trace amount of actinomycin will affect the RNA synthesis of plant pathogens, thereby reducing the generation of drug resistance. By adopting the above structure, the drug resistance of Phytophthora infestans of potatoes to the compound agent of metalaxyl-M and azoxystrobin can be effectively reduced, and the control effect can be improved.

[0018] In the present invention, the drug components and fertilizer components are compounded to prepare a slow-release agent, which can ensure that the fertilizer nutrients and the effective components of agricultural drugs do not decrease during the action, and can play the synergistic effect of both, and can effectively increase the yield of potatoes. Detailed implementation mode

[0019] Next, in combination with the embodiments of the present invention, the technical solutions of the invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0020] The Eupatorium adenophorum extract used in the embodiments of the present invention can be selected and purchased on the market, or can be prepared by the following method: The dried stems and leaves of Eupatorium adenophorum and 75% ethanol are soaked at room temperature for 8 hours according to the ratio of 1 g / 5 mL, filtered, and the filtrate is dried at low temperature and pulverized to obtain the Eupatorium adenophorum extract. The Eupatorium adenophorum extract used in the embodiments of the present invention is prepared by the above method.

[0021] Example 1

[0022] A granular medicine fertilizer for reducing the drug resistance to metalaxyl-M and azoxystrobin, comprising drug components, fertilizer components, a binder and a slow-release agent. By weight percentage, the drug components are 0.2%, the binder is 6%, the slow-release agent is 5%, and the balance is fertilizer components; By weight percentage, the drug components include 15% metalaxyl-M (active ingredient, calculated as 100%), 5% azoxystrobin (active ingredient, calculated as 100%), 0.02 mg / kg of actinomycin (active ingredient, calculated as 100%), 1% of Eupatorium adenophorum extract, 20% of β-cyclodextrin, and the balance is an inert carrier.

[0023] The fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15), the binder is carboxymethyl cellulose FH9; the slow-release agent is selected from polyvinyl alcohol 5000; the fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15); and the inert carrier is kaolin.

[0024] The combination of metalaxyl-M and azoxystrobin has preventive and control effects on a variety of diseases, including root rot, stem base rot, anthracnose, downy mildew, damping-off, damping-off, sheath blight, and vine blight. The binder, slow-release agent, fertilizer, and inert carrier can be optimized based on the specific pests and diseases to be controlled. For example, the binder can be one or more of xanthan gum, polyvinyl pyrrolidone, sodium carboxymethyl cellulose, cross-linked sodium carboxymethyl cellulose, and chitosan. The slow-release agent can be one or more of polyvinyl alcohol, polyethylene, polypropylene glycol ester, and epoxy resin. The fertilizer can be one or more of nitrogen fertilizer, phosphate fertilizer, and potash fertilizer. The inert carrier can be one or more of kaolin, white carbon black, bentonite, vermiculite, diatomaceous earth, attapulgite, zeolite, and sepiolite. The composite prepared in the embodiments of the present invention is primarily targeted at potato late blight, so specific examples of optimized binders, slow-release agents, fertilizers, and inert carriers are not listed. Those skilled in the art can select appropriate ones as needed.

[0025] The preparation method thereof comprises the following steps:

[0026] (1) 50% of metalaxyl-M and dactinomycin are mixed uniformly for later use, and then, at 30° C., the mixture of cyclodextrin and the mixture of metalaxyl-M and dactinomycin is mixed in a ratio of 1:1, and the mixture of metalaxyl-M and dactinomycin is slowly added to a saturated aqueous solution of cyclodextrin under constant stirring, and stirred at 50° C. for 6 hours to ensure that the inclusion reaction is complete, thereby obtaining a mixture;

[0027] (2) uniformly mixing the mixture prepared in step 1 with the remaining metalaxyl-M, azoxystrobin and Eupatorium adenophorum extract, then adding an inert carrier and mixing uniformly to obtain a pharmaceutical composition;

[0028] (3) uniformly mixing the obtained drug component, the crushed fertilizer component, and the binder, and granulating the mixture using a disc granulator;

[0029] (4) The granulated product is coated with a sustained-release agent.

[0030] Example 2

[0031] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a drug component, a fertilizer component, a binder and a sustained-release agent, wherein, by weight, the drug component comprises 0.05%, the binder comprises 1%, the sustained-release agent comprises 8%, and the remainder is the fertilizer component; the drug component comprises, by weight, 1% metalaxyl-M (active ingredient, based on 100%), 10% azoxystrobin (active ingredient, based on 100%), 0.01 mg / kg of dactinomycin (active ingredient, based on 100%), 2% of an Eupatorium adenophorum extract, 6% of beta-cyclodextrin, and the remainder is an inert carrier.

[0032] The fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15), the binder is carboxymethyl cellulose FH9; the slow-release agent is selected from polyvinyl alcohol 5000; the fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15); and the inert carrier is kaolin.

[0033] The preparation method thereof comprises the following steps:

[0034] (1) 50% of metalaxyl-M and dactinomycin are mixed uniformly for later use, and then the mixture of cyclodextrin and metalaxyl-M and dactinomycin is mixed at 35° C. in a ratio of 1:1. The mixture of metalaxyl-M and dactinomycin is slowly added to a saturated aqueous solution of cyclodextrin under constant stirring, and stirred at 60° C. for 6 hours to ensure that the inclusion reaction is complete, thereby obtaining a mixture;

[0035] (2) uniformly mixing the mixture prepared in step 1 with the remaining metalaxyl-M, azoxystrobin and Eupatorium adenophorum extract, then adding an inert carrier and mixing uniformly to obtain a pharmaceutical composition;

[0036] (3) uniformly mixing the obtained drug component, the crushed fertilizer component, and the binder, and granulating the mixture using a disc granulator;

[0037] (4) The granulated product is coated with a sustained-release agent.

[0038] Example 3

[0039] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a pharmaceutical component, a fertilizer component, a binder, and a sustained-release agent. The pharmaceutical component comprises, by weight, 0.3% of the pharmaceutical component, 10% of the binder, 6% of the sustained-release agent, and the remainder of the fertilizer component. The pharmaceutical component comprises, by weight, 5% of metalaxyl-M (active ingredient, based on 100%), 1% of azoxystrobin (active ingredient, based on 100%), 0.0.02 mg / kg of dactinomycin, 3% of an Eupatorium adenophorum extract, 6% of β-cyclodextrin, and the remainder being an inert carrier. The preparation method is the same as that of Example 1.

[0040] Example 4

[0041] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a pharmaceutical component, a fertilizer component, a binder, and a sustained-release agent. The pharmaceutical component comprises, by weight, 0.05% of the pharmaceutical component, 1% of the binder, 1% of the sustained-release agent, and the remainder of the fertilizer component. The pharmaceutical component comprises, by weight, 20% of metalaxyl-M (active ingredient, based on 100%), 20% of azoxystrobin (active ingredient, based on 100%), 0.02 mg / kg of dactinomycin, 2% of Eupatorium adenophorum extract, 40% of β-cyclodextrin, and the remainder being an inert carrier. The preparation method is the same as that of Example 1.

[0042] Example 5

[0043] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a pharmaceutical component, a fertilizer component, a binder, and a sustained-release agent. The pharmaceutical component comprises, by weight, 0.1% of the pharmaceutical component, 6% of the binder, 8% of the sustained-release agent, and the remainder of the fertilizer component. The pharmaceutical component comprises, by weight, 12% of metalaxyl-M (active ingredient, based on 100%), 4% of azoxystrobin (active ingredient, based on 100%), 0.01 mg / kg of dactinomycin, 1% of an Eupatorium adenophorum extract, 16% of β-cyclodextrin, and the remainder being an inert carrier. The preparation method is the same as that of Example 1.

[0044] Example 6

[0045] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a drug component, a fertilizer component, a binder and a sustained-release agent, wherein, by weight, the drug component comprises 0.2%, the binder comprises 6%, the sustained-release agent comprises 5%, and the balance is the fertilizer component; the drug component comprises, by weight, 15% metalaxyl-M (active ingredient, calculated as 100%), 5% azoxystrobin (active ingredient, calculated as 100%), 0.02 mg / kg of dactinomycin (active ingredient, calculated as 100%), 1% of an Eupatorium adenophorum extract, and the balance is an inert carrier.

[0046] The fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15), the binder is carboxymethyl cellulose FH9; the slow-release agent is selected from polyvinyl alcohol 5000; the fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15); and the inert carrier is kaolin.

[0047] Example 7

[0048] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a drug component, a fertilizer component, a binder and a sustained-release agent, wherein, by weight, the drug component comprises 0.2%, the binder comprises 6%, the sustained-release agent comprises 5%, and the balance is the fertilizer component; the drug component comprises, by weight, 15% metalaxyl-M (active ingredient, based on 100%), 5% azoxystrobin (active ingredient, based on 100%), 0.02 mg / kg of dactinomycin (active ingredient, based on 100%), 3% of Bacillus subtilis powder, and the balance is an inert carrier.

[0049] The fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15), the binder is carboxymethyl cellulose FH9; the slow-release agent is selected from polyvinyl alcohol 5000; the fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15); and the inert carrier is kaolin.

[0050] The preparation method is basically the same as that of Example 1, except that Bacillus subtilis powder is added in step (2).

[0051] The Bacillus subtilis powder used in this example is commercially available Bacillus subtilis for plant disease and insect pest control, with an active ingredient content of 10 billion spores per gram.

[0052] Comparative Example 1

[0053] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a drug component, a fertilizer component, a binder and a sustained-release agent, wherein, by weight, the drug component comprises 0.2%, the binder comprises 6%, the sustained-release agent comprises 5%, and the remainder is the fertilizer component; and the drug component comprises, by weight, 15% of metalaxyl-M (active ingredient, based on 100%), 5% of azoxystrobin (active ingredient, based on 100%), 20% of beta-cyclodextrin, and the remainder is an inert carrier.

[0054] The fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15), the binder is carboxymethyl cellulose FH9; the slow-release agent is selected from polyvinyl alcohol 5000; the fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15); and the inert carrier is kaolin.

[0055] Comparative Example 2

[0056] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a drug component, a fertilizer component, a binder and a sustained-release agent, wherein, by weight, the drug component comprises 0.2%, the binder comprises 6%, the sustained-release agent comprises 5%, and the balance is the fertilizer component; the drug component comprises, by weight, 15% metalaxyl-M (active ingredient, based on 100%), 5% azoxystrobin (active ingredient, based on 100%), 1% Eupatorium adenophorum extract, 20% beta-cyclodextrin, and the balance is an inert carrier.

[0057] The fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15), the binder is carboxymethyl cellulose FH9; the slow-release agent is selected from polyvinyl alcohol 5000; the fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15); and the inert carrier is kaolin.

[0058] Comparative Example 3

[0059] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a drug component, a fertilizer component, a binder and a sustained-release agent, wherein, by weight, the drug component comprises 0.2%, the binder comprises 6%, the sustained-release agent comprises 5%, and the remainder is the fertilizer component; the drug component comprises, by weight, 15% metalaxyl-M (active ingredient, based on 100%), 5% azoxystrobin (active ingredient, based on 100%), 0.02 mg / kg of dactinomycin (active ingredient, based on 100%), 20% beta-cyclodextrin, and the remainder is an inert carrier.

[0060] The fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15), the binder is carboxymethyl cellulose FH9; the slow-release agent is selected from polyvinyl alcohol 5000; the fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15); and the inert carrier is kaolin.

[0061] Comparative Example 4

[0062] A granular fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, comprising a drug component, a fertilizer component, a binder and a sustained-release agent, wherein, by weight, the drug component comprises 0.2%, the binder comprises 6%, the sustained-release agent comprises 5%, and the remainder is the fertilizer component; the drug component comprises, by weight, 15% metalaxyl-M (active ingredient, based on 100%), 5% azoxystrobin (active ingredient, based on 100%), 0.02 mg / kg of dactinomycin (active ingredient, based on 100%), and the remainder is an inert carrier.

[0063] The fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15), the binder is carboxymethyl cellulose FH9; the slow-release agent is selected from polyvinyl alcohol 5000; the fertilizer is nitrogen-phosphorus-potassium compound fertilizer (N15-P20-K15); and the inert carrier is kaolin.

[0064] The preparation method thereof comprises the following steps: The preparation method thereof comprises the following steps:

[0065] (1) uniformly mixing metalaxyl-M, azoxystrobin, dactinomycin, and an Eupatorium adenophorum extract, then adding an inert carrier and mixing uniformly to obtain a pharmaceutical composition;

[0066] (2) uniformly mixing the obtained drug component, the crushed fertilizer component, and the binder, and granulating the mixture using a disc granulator;

[0067] (3) The granulated product is coated with a sustained-release agent.

[0068] Field efficacy trials

[0069] In a Shangqiu experimental field, where potatoes are grown year-round, a combination of metalaxyl-M and azoxystrobin was used to control potato late blight. Zhengshu No. 6 was used. In early March, the land was deep plowed and finely harrowed, and furrows were dug to create beds with a row spacing of 60 cm, a furrow depth of 10 cm, and a spacing of 20-25 plants. During land preparation, 3,000 kg / hm² of well-rotted organic fertilizer and 200 kg / hm² of nitrogen, phosphorus, and potassium compound fertilizer were applied at a sowing density of 45,000 plants / hm². In the early stages of potato formation, a topdressing of 100 kg / hm² of nitrogen, phosphorus, and potassium compound fertilizer was applied. If potato late blight occurs before topdressing, a single application of 50 kg / hm² of nitrogen, phosphorus, and potassium compound fertilizer was applied at the early stages of the disease. This was broadcasted, and a further 50 kg / hm² of fertilizer was applied during topdressing, maintaining a total topdressing rate of 100 kg / hm². If potato late blight occurs after topdressing, no drugs are used. The nitrogen, phosphorus and potassium compound fertilizer used is set to 7 treatments, namely Example 1, Example 7, Comparative Examples 1-4 and a blank control. The nitrogen, phosphorus and potassium compound fertilizer used in the blank control is N15-P20-K15.

[0070] Before applying pesticides, investigate the disease base and the control effect. 7 days and 14 days after the initial stage of potato late blight, sample five diagonal points in each plot, and select three plants at each point. Investigate the disease level and disease index of all leaves, and calculate the control effect based on the disease index:

[0071] Potato late blight grading standard: Level 0: no lesions; Level 1: lesion area accounts for less than 5% of the total leaf area; Level 3: lesion area accounts for 6% to 10% of the total leaf area; Level 5: lesion area accounts for 11% to 20% of the total leaf area; Level 7: lesion area accounts for 21% to 50% of the total leaf area; Level 9: lesion area accounts for more than 50% of the total leaf area.

[0072] Calculation method of drug efficacy: Use the following formula to calculate the disease index and preventive efficacy:

[0073] Disease index (%) = ∑(number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × 9) × 100

[0074] Relative control effect (%) = (disease index after spraying in the blank control area - disease index after spraying in the medicament - fertilizer treatment area) / disease index after spraying in the blank control area × 100.

[0075] Weigh the potatoes harvested from each treatment area and calculate the yield increase rate.

[0076] Yield increase rate (%) = (potato yield per mu in the blank control area - potato yield per mu in the medicament - fertilizer treatment area) / potato yield per mu in the blank control area × 100.

[0077] The specific effects of the medicament - fertilizer on the control of potato late blight and its yield are shown in the following table.

[0078]

[0079] As can be seen from the above table, the potato late blight in the plots used in this experiment has developed obvious resistance to the compound of metalaxyl - M and azoxystrobin, and there is a significant decrease in the control effect compared with the records in the literature. Adding dactinomycin and Eupatorium adenophorum extract can effectively improve the control effect of the compound of metalaxyl - M and azoxystrobin on potato late blight. From Comparative Examples 2 and 3, it can be seen that adding dactinomycin or Eupatorium adenophorum extract alone can also improve the control effect, but the improvement is limited. The combination of dactinomycin and Eupatorium adenophorum extract has an obvious improvement effect and a significant effect.

[0080] In addition, the combination of dactinomycin and Eupatorium adenophorum extract not only improves the control effect, but also increases the yield of potatoes. The effect on increasing the yield is obvious, and the yield increase rate reaches more than 38%. It can be seen that the medicament - fertilizer of the present invention can effectively solve the resistance of potato late blight and improve the control effect.

[0081] In addition, from Comparative Example 4, it can be seen that the dactinomycin used in the present invention is a bright red or orange - red crystal, without odor, hygroscopic, and becomes unstable when exposed to light and heat. Therefore, the present invention uses β - cyclodextrin treatment, which can effectively improve the stability of dactinomycin. At the same time, using 50% metalaxyl - M and dactinomycin treated with β - cyclodextrin can form a secondary slow - release effect, which can effectively increase the action time of metalaxyl - M and thus improve the control effect.

[0082] Bacillus subtilis has high resistance to continuous cropping. During its growth period, it can secrete bacteriocins, organic acid substances, and lipopeptide compounds, which can inhibit the growth of pathogenic bacteria; sterilize and lyse bacteria: Bacillus subtilis can consume and decompose pathogenic bacteria, causing their hyphae to break, thus preventing the further harm of the bacteria to the plants; regulate growth: Bacillus subtilis can produce substances similar to plant growth hormones, which have a promoting effect on plant growth. Using compound Bacillus subtilis in medicine-fertilizer can effectively improve the control effect.

[0083] The medicine-fertilizer prepared in Example 1 of the present invention was used in a field experiment. When continuously used 4 times in potato planting, the control effect on potato late blight was above 80%, and it had good drug resistance.

[0084] Although the embodiments of the present invention have been described above, for those skilled in the art, the modifications and substitutions made without departing from the principles and spirit of the present invention all fall within the scope claimed by the present invention.

Claims

1. A granular medicated fertilizer for reducing resistance to metalaxyl-M and azoxystrobin, characterized in that: The invention comprises a drug component, a fertilizer component, a binder and a sustained-release agent, wherein, by weight, the drug component comprises 0.05-0.3%, the binder comprises 1-10%, the sustained-release agent comprises 1-10%, and the balance is the fertilizer component; the drug component comprises, by weight, 1-20% metalaxyl-M, 1-20% azoxystrobin, 0.01-0.02 mg / kg of dactinomycin, 1-3% of Eupatorium adenophorum extract, 2-40% of cyclodextrin, and the balance is an inert carrier; The binder is one or more selected from the group consisting of xanthan gum, polyvinyl pyrrolidone, sodium carboxymethyl cellulose, cross-linked sodium carboxymethyl cellulose, and chitosan; the sustained-release agent is one or more selected from the group consisting of polyvinyl alcohol, polyethylene, polypropylene glycol ester, and epoxy resin; and the drug resistance is drug resistance to metalaxyl-M and azoxystrobin for controlling potato late blight. The preparation method thereof comprises the following steps: (1) 50% of metalaxyl-M and dactinomycin are mixed uniformly for later use, and then a sample is taken at 25-35° C. according to the amount of the mixture of cyclodextrin and metalaxyl-M and dactinomycin being 1:

1. The mixture of metalaxyl-M and dactinomycin is slowly added to a saturated aqueous solution of cyclodextrin under continuous stirring, and stirred at 50-60° C. for more than 5 hours to ensure that the inclusion reaction is complete, thereby obtaining a mixture; (2) uniformly mixing the mixture prepared in step 1 with the remaining metalaxyl-M, azoxystrobin and Eupatorium adenophorum extract, then adding an inert carrier and mixing uniformly to obtain a pharmaceutical composition; (3) uniformly mixing the obtained drug component, the crushed fertilizer component, and the binder, and granulating; (4) The granulated product is coated with a sustained-release agent to obtain: The medicinal ingredients also include 2-5% of Bacillus subtilis powder; The preparation method of the Eupatorium adenophorum extract comprises the following steps: drying the stems and leaves of Eupatorium adenophorum and soaking them in 75% ethanol at a ratio of 1 g / 5 mL at room temperature for 8 hours, filtering the mixture, drying the filtrate at low temperature, and crushing the mixture to obtain the Eupatorium adenophorum extract.

2. A granular medicine fertilizer for reducing resistance to metalaxyl-M and azoxystrobin according to claim 1, characterized in that: The fertilizer is selected from one or more of nitrogen fertilizer, phosphorus fertilizer and potash fertilizer.

3. A granular medicine fertilizer for reducing resistance to metalaxyl-M and azoxystrobin according to claim 1, characterized in that: The inert carrier includes one or more of kaolin, white carbon black, bentonite, vermiculite, diatomaceous earth, attapulgite, zeolite and sepiolite.

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