Composite microbial agent, application of composite microbial agent and method for treating high-concentration organic wastewater

Abstract

The application provides a composite microbial agent, application of the composite microbial agent and a treatment method of high-concentration organic wastewater. The active component of the composite microbial agent provided in the first aspect of the application comprises two or three of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain. The composite microbial agent provided in the first aspect of the application can be used for biological decontamination treatment of high-concentration organic wastewater, so as to realize degradation of organic pollutants in the high-concentration organic wastewater and pH value adjustment, thereby avoiding increase of the load of a municipal sewage treatment system.

Description

[0001] The present disclosure claims priority to the Chinese patent application No. 202211419596.X, filed on November 14, 2022, and entitled “Composite microbial agent, application of composite microbial agent, and treatment method of distiller’s grain leachate”, the entire content of which is incorporated herein by reference. TECHNICAL FIELD

[0002] The present application relates to the technical field of biological microbial agents, in particular to a composite microbial agent, application of the composite microbial agent, and treatment method of high-concentration organic wastewater. BACKGROUND

[0003] Distiller’s grain is a solid waste generated in the production of liquor. Distiller’s grain is complex in composition and contains a large amount of soluble organic pollutants. Due to the high water content of distiller’s grain, if not treated in time, wastewater will seep out during stacking, resulting in distiller’s grain leachate. The distiller’s grain leachate contains a large amount of organic pollutants and is highly acidic, belonging to high-concentration organic wastewater. If the distiller’s grain leachate is not pretreated before being discharged into the municipal sewage treatment system, it will increase the energy consumption of subsequent decontamination and the load of the treatment system. At present, there is also a lack of research on the use of biological reinforcement technology to treat distiller’s grain leachate.

[0004] Therefore, there is an urgent need for a composite microbial agent, application of the composite microbial agent, and treatment method of high-concentration organic wastewater. SUMMARY

[0005] The first aspect of the present application provides a composite microbial agent, wherein the active components of the composite microbial agent include two or three of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain, and Bacillus licheniformis MX8 strain,

[0006] The Paecilomyces varioti MM2 strain is preserved in the China Microbial Preservation Management Committee on July 8, 2013, and the preservation number is CGMCC NO. 7903; the Aspergillus fumigatus MM6 strain is preserved in the China Microbial Preservation Management Committee on July 8, 2013, and the preservation number is CGMCC NO. 7904; the Candida rugosa MJ4 strain is preserved in the China Microbial Preservation Management Committee on July 8, 2013, and the preservation number is CGMCC NO. 7902; and the Bacillus licheniformis MX8 strain is preserved in the China Microbial Preservation Management Committee on July 8, 2013, and the preservation number is CGMCC NO. 7901.

[0007] The composite microbial agent provided in the first aspect of the present application can be used for biological decontamination treatment of the leaching liquor of vinasse, so as to realize degradation of organic pollutants in high-concentration organic wastewater (i.e., the leaching liquor of vinasse) and pH value adjustment, so as to avoid increasing the load of a municipal sewage treatment system.

[0008] In some optional embodiments of the first aspect of the present application, the active components of the composite microbial agent are the Bacillus licheniformis MX8 strain and the Aspergillus fumigatus MM6 strain.

[0009] In some optional embodiments of the first aspect of the present application, the active components of the composite microbial agent are two or three strains, wherein the composite microbial agent comprises the Aspergillus fumigatus MM6 strain and the Paecilomyces varioti MM2 strain.

[0010] In some optional embodiments of the first aspect of the present application, the active components of the composite microbial agent are the Bacillus licheniformis MX8 and the Candida rugosa MJ4 strain.

[0011] In some optional embodiments of the first aspect of the present application, the active components of the composite microbial agent are the Bacillus licheniformis MX8, the Candida rugosa MJ4 strain and the Paecilomyces varioti MM2 strain.

[0012] In some optional embodiments of the first aspect of the present application, the active component of the complex microbial agent is two or three strains, and the complex microbial agent comprises Aspergillus fumigatus MM6 strain.

[0013] The second aspect of the present application provides an application of the complex microbial agent in the first aspect of the present application in the decontamination treatment of the distiller's grains leachate.

[0014] In some optional embodiments of the second aspect of the present application, the other strains remaining after removing the strains included in the complex microbial agent from the four strains of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain are also added to the distiller's grains leachate for decontamination.

[0015] In some optional embodiments of the second aspect of the present application, the ratio of the colony forming units of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain in the decontamination treatment of the distiller's grains leachate is (7.55~8): (158~159):1:(1141~1142).

[0016] The third aspect of the present application provides a treatment method of high-concentration organic wastewater, wherein the high-concentration organic wastewater is distiller's grains leachate, and the treatment method comprises:

[0017] The distiller's grains leachate is diluted and sterilized to obtain a to-be-treated liquid;

[0018] The complex microbial agent in the first aspect of the present application is inoculated into the to-be-treated liquid to form a treatment liquid;

[0019] The treatment liquid is cultured at 30℃~37℃ for 60~80 hours.

[0020] The treatment method of high-concentration organic wastewater provided in the third aspect of the present application realizes decontamination treatment of the distiller's grains leachate by using biological enhancement technology, and reduces the content of organic pollutants in the distiller's grains leachate and adjusts the pH value of the distiller's grains leachate. DETAILED DESCRIPTION

[0021] The application will be further described in detail below with reference to examples. It should be understood that the specific examples described herein are merely used to explain the application and not to limit the application.

[0022] Distiller's grains is a solid waste produced in the process of liquor production. Distiller's grains is complex in composition and contains a large amount of soluble organic pollutants. Current treatment technologies for distiller's grains mainly include preparation of energy from distiller's grains, production of feed from distiller's grains, production of chemical raw materials from distiller's grains, production of materials for use from distiller's grains, production of food from distiller's grains, and production of organic fertilizer from distiller's grains. Due to high water content of distiller's grains, if not treated in time, wastewater will seep out during the stacking process, resulting in distiller's grains leachate. The distiller's grains leachate contains a large amount of organic pollutants, has high acidity, and has high concentration of pollutants (COD), which seriously pollutes the environment.

[0023] The distiller's grains leachate produced during the stacking process of distiller's grains is high-concentration organic wastewater. If the distiller's grains leachate is not decontaminated and a large amount of distiller's grains leachate is directly discharged into the municipal sewage treatment system, the organic matter content in the original wastewater will be changed, the COD content of the wastewater will be increased, and the high-concentration pollutants in the wastewater of the municipal sewage treatment system will be decomposed and treated to meet the national discharge standard, which will increase the energy consumption and the load of the treatment system. If the distiller's grains leachate is properly decontaminated and then discharged into the municipal sewage treatment system after the high-concentration pollutants are reduced, the wastewater treatment investment and operation cost of the municipal sewage treatment system can be reduced, which is of great significance to the development of the distiller's grains processing industry. At present, there is a lack of research on the treatment of distiller's grains leachate by using biological enhancement technology.

[0024] In view of this, the application is proposed.

[0025] The following describes the composite microbial agent, application of the composite microbial agent and treatment method of high-concentration organic wastewater provided by the application in detail through specific examples.

[0026]

EXAMPLE

[0027] 1. Experimental materials

[0028] 1.1 Distiller's grains leachate:

[0029] The distiller's grains leachate was the floating water produced during the stacking of distiller's grains provided by Guizhou Maotai Distillery (Group) Co., Ltd.

[0030] 1.2 Culture medium:

[0031] 1.2.1 YPD solid culture medium:

[0032] The YPD solid culture medium contains 10 g / L of yeast extract, 20 g / L of peptone, 20 g / L of glucose, and 2 wt% of agar powder.

[0033] 1.2.2 Luria-Bertani solid medium (hereinafter referred to as LB solid medium):

[0034] containing tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 10 g / L, and agar powder 2 wt%.

[0035] 1.2.3 Yeast extract powder peptone glucose liquid medium (hereinafter referred to as YPD liquid medium):

[0036] containing yeast extract 10 g / L, peptone 20 g / L, and glucose 20 g / L.

[0037] 1.2.4 Luria-Bertani liquid medium (hereinafter referred to as LB liquid medium):

[0038] containing tryptone 10 g / L, yeast extract 5 g / L, and sodium chloride 10 g / L.

[0039] The above-mentioned mediums are sterilized at 121 ℃ for 20 minutes.

[0040] 2. Single strain activation

[0041] The Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, and Candida rugosa MJ4 strain preserved on a plate are inoculated in YPD solid medium and cultured at 30 ℃ for 72 h for activation, and the Bacillus licheniformis MX8 strain is inoculated in LB solid medium and cultured at 37 ℃ for 72 h for activation.

[0042] The Paecilomyces varioti MM2 strain is preserved in the China General Microbiological Culture Collection Center on July 8, 2013, and the preservation number is CGMCC NO. 7903; the Aspergillus fumigatus MM6 strain is preserved in the China General Microbiological Culture Collection Center on July 8, 2013, and the preservation number is CGMCC NO. 7904; the Candida rugosa MJ4 strain is preserved in the China General Microbiological Culture Collection Center on July 8, 2013, and the preservation number is CGMCC NO. 7902; and the Bacillus licheniformis MX8 strain is preserved in the China General Microbiological Culture Collection Center on July 8, 2013, and the preservation number is CGMCC NO. 7901.

[0043] 3. Strain expansion culture

[0044] 3.1, the activated Paecilomyces varioti MM2 strain was inoculated into YPD liquid medium, and placed in a constant temperature incubator at 30℃ for 72h to obtain the seed liquid of Paecilomyces varioti MM2 strain, and the concentration of the seed liquid was 9.3×10 5 CFU / mL.

[0045] 3.2, the activated Aspergillus fumigatus MM6 strain was inoculated into YPD liquid medium, and placed in a constant temperature incubator at 30℃ for 72h to obtain the seed liquid of Aspergillus fumigatus MM6 strain, and the concentration of the seed liquid was 1.9×10 7 CFU / mL.

[0046] 3.3, the activated Candida rugosa MJ4 strain was inoculated into YPD liquid medium, and placed in a constant temperature incubator at 30℃ for 72h to obtain the seed liquid of Candida rugosa MJ4 strain, and the concentration of the seed liquid was 1.2×10 5 CFU / mL.

[0047] 3.4, the activated Bacillus licheniformis MX8 strain was inoculated into LB liquid medium, and placed in a constant temperature incubator at 37℃ for 72h to obtain the seed liquid of Candida rugosa MJ4 strain, and the concentration of the seed liquid was 1.37×10 8 CFU / mL.

[0048] 4. Decontamination treatment of high-concentration organic wastewater (i.e. distiller's grains leachate)

[0049] 4.1, a plurality of composite microbial agents with different strain combinations provided by the first aspect of the application were used to decontaminate the distiller's grains leachate to verify the decontamination ability of each of the plurality of composite microbial agents with different strain combinations provided by the first aspect of the application on the distiller's grains leachate.

[0050] The method for treating high-concentration organic wastewater comprises:

[0051] S10: The distiller's grains leachate was diluted 100 times with ultrapure water, and the diluted distiller's grains leachate was placed in an autoclave for high-temperature sterilization treatment at 121℃ for 20 minutes to obtain a liquid to be treated;

[0052] S20: inoculate the multiple composite microbial inoculants with different strain combinations respectively into the to-be-treated liquid obtained in step S10 to obtain multiple treatment liquids containing the composite microbial inoculants, the multiple treatment liquids containing the composite microbial inoculants correspond to the multiple composite microbial inoculants with different strain combinations one-to-one, the multiple to-be-inoculated to-be-treated liquids corresponding to the multiple composite microbial inoculants with different strain combinations are obtained by diluting the same multiple of the same volume of the lees filtrate, the multiple composite microbial inoculants inoculated into the multiple treatment liquids are different from each other, the total inoculation amount of each of the multiple composite microbial inoculants is the same and is 1 / 300 (V / V), and the inoculation amounts (i.e., the inoculation volumes) of the seed liquids of the multiple strains in the multiple composite microbial inoculants are equal;

[0053] In step S20, the seed liquids of the multiple strains in the composite microbial inoculants can be mixed according to the inoculation amounts to obtain a mixed microbial liquid, and then the mixed microbial liquid is inoculated into the to-be-treated liquid obtained in step S10; or the seed liquids of the multiple strains in the composite microbial inoculants can be inoculated into the to-be-treated liquid obtained in step S10 respectively.

[0054] S30: incubate the treatment liquid in an incubator at 37°C for 72 hours.

[0055] 4.2 Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain, and Bacillus licheniformis MX8 strain are respectively used for decontamination treatment of the lees filtrate alone to form a control for the decontamination ability of the composite microbial inoculant.

[0056] The method for treating high-concentration organic wastewater comprises the following steps:

[0057] S10: dilute the lees filtrate 100 times with ultrapure water, place the diluted lees filtrate in an autoclave for high-temperature sterilization treatment, sterilize at 121°C for 20 minutes to obtain a to-be-treated liquid;

[0058] S20: Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain were inoculated into the to-be-treated liquid obtained in step S10 respectively to obtain four kinds of treatment liquids containing different single strains, and the to-be-inoculated to-be-treated liquids corresponding to different strains were all obtained by diluting the same volume of vinasse filtrate by the same multiple, and the inoculation amount of the seed liquid of each strain was 1 / 300 (V / V);

[0059] S30: The treatment liquid was cultured in a constant temperature incubator at 37°C for 72 hours.

[0060] 4.1 and 4.2 parts of the vinasse filtrate were obtained from the same batch, had the same volume, and were diluted by the same multiple.

[0061] 5, Decontamination treatment result determination

[0062] 5.1 pH value determination:

[0063] The pH value of the treatment liquid after step S30 was measured by a pH meter

[0064] 5.2 Chemical oxygen demand (COD) determination:

[0065] The initial COD content of the to-be-treated liquid in step S10 was determined by potassium dichromate method, the residual COD content of the treatment liquid after step S30 in 4.1 and 4.2 parts was determined by potassium dichromate method, and the COD degradation rate of the vinasse filtrate in different examples in 4.1 and 4.2 parts was calculated.

[0066] 5.3 Ammonia nitrogen (NH3-N) determination

[0067] The NH3-N content of the to-be-treated liquid in step S10 was determined by salicylic acid spectrophotometry, and the residual NH3-N content of the treatment liquid after step S30 in 4.1 and 4.2 parts was determined by salicylic acid spectrophotometry, and the NH3-N degradation rate of the vinasse filtrate in different examples in 4.1 and 4.2 parts was calculated.

[0068] 6, Experimental results and analysis

[0069] Table 1 COD degradation rate of single strain on vinasse filtrate

[0070]

[0071] As can be seen from Table 1, the COD degradation effects of the four strains on the wine lees permeate are Aspergillus fumigatus (MM6) > Paecilomyces varioti (MM2) > Candida humilis (MJ4) > Bacillus licheniformis (MX8). Bacillus licheniformis (MX8) does not have the ability to degrade COD when inoculated alone.

[0072] Table 2 Comparison of COD degradation rates of single strain and complex microbial agent on wine lees permeate

[0073]

[0074] As can be seen from Table 2, the complex microbial agent containing two, three or four strains has good COD degradation effect on the wine lees permeate. The COD degradation effect of various complex microbial agents is better than that of any single strain. As shown in Example 5, the COD degradation rate of the complex microbial agent is increased by 40.625% compared with the single strain. In Example 8, the complex microbial agent containing Paecilomyces varioti (MM2) and Aspergillus fumigatus (MM6) has the highest COD degradation rate on the wine lees permeate, which is as high as 64.14%. As can be seen from Table 2, the COD degradation rate in Example 8 is the best. The complex microbial agent in Example 8 includes Bacillus licheniformis (MX8) and Aspergillus fumigatus (MM6), which are also the decontamination active components of the complex microbial agent.

[0075] In some examples, the active components of the complex microbial agent are two or three strains, wherein the complex microbial agent includes Aspergillus fumigatus (MM6) and Paecilomyces varioti (MM2). As can be seen from Table 2, when the complex microbial agent includes Aspergillus fumigatus (MM6) and Paecilomyces varioti (MM2), such as in Examples 11, 12, 15 and 16, the degradation rate of the complex microbial agent on the wine lees permeate is more than 60%.

[0076] Table 3 NH3-N degradation rate of single strain on wine lees permeate

[0077]

[0078] As can be seen from Table 3, the NH3-N degradation effect of the four strains on the wine lees leachate is Aspergillus fumigatus (MM6) > Paecilomyces varioti (MM2) > Candida rugosa (MJ4) > Bacillus licheniformis (MX8). Bacillus licheniformis (MX8) does not have the ability to degrade NH3-N when inoculated alone.

[0079] Table 4 Comparison of NH3-N degradation rates of single strain and complex microbial agent on wine lees leachate

[0080]

[0081] As can be seen from Table 4, when the active components of the complex microbial agent in Example 5 are Bacillus licheniformis (MX8) and Candida rugosa (MJ4) strains, the NH3-N degradation effect of the complex microbial agent is superior to that of any single strain.

[0082] In Example 13, the active components of the complex microbial agent are Bacillus licheniformis (MX8), Candida rugosa (MJ4) and Paecilomyces varioti (MM2) strains, and the NH3-N degradation effect of the complex microbial agent is superior to that of any single strain.

[0083] In some examples, the active components of the complex microbial agent are two or three strains, and the complex microbial agent includes the Aspergillus fumigatus (MM6) strain. As can be seen from Table 4, the complex microbial agent includes the Aspergillus fumigatus (MM6) strain, for example, Examples 11 to 16, and the NH3-N degradation rate of the complex microbial agent is higher, between 49% and 55%.

[0084] Table 5 pH value of the treated liquor after decontamination treatment of single strain and complex microbial agent on wine lees leachate

[0085]

[0086] As can be seen from Table 5, the pH values of Examples 1 to 13 are all between 6 and 9. According to the water quality index in the Fermentation Alcohol and Distilled Spirits Industry Wastewater Pollutant Discharge Standard (GB 27631-2011), the pH value of the treated liquor meets the direct discharge standard, i.e. both single strain and complex microbial agent can make the pH value of the wine lees leachate rise to the range of the discharge standard.

[0087] The second aspect of the present application provides an application of the composite microbial agent provided by the first aspect of the present application in the decontamination treatment of the wine lees filtrate.

[0088] In some optional embodiments, other strains remaining after removing the strains included in the composite microbial agent from the four strains of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain are also added to the wine lees filtrate for decontamination. As known from the above-mentioned Example 13, the composite microbial agent includes the four strains of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain. The composite microbial agent of Example 13 has a positive effect on the decontamination treatment of the wine lees filtrate, especially in terms of COD degradation, NH3-N degradation and pH value increase.

[0089] In some optional embodiments, the ratio of colony forming units of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain in the decontamination treatment of the wine lees filtrate is 7.55-8): (158-159): 1: (1141-1142).

[0090] In some examples of these embodiments, the inoculation volume of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain is the same, and the concentrations of the four strains are different. The concentration of the seed liquid of Paecilomyces varioti MM2 strain is 9.3x10 5 CFU / mL, the concentration of the seed liquid of Aspergillus fumigatus MM6 strain is 1.9x10 7CFU / mL, the seed liquid concentration of the Candida rugosa MJ4 strain was 1.2×10 5 CFU / mL, the seed liquid concentration of the Bacillus licheniformis MX8 strain was 1.37×10 8 CFU / mL. CFU is Colony Forming Unit. The colony forming units of each strain inoculated can be calculated by the inoculation volume and the seed liquid concentration of each strain.

[0091] The third aspect of the present application provides a high-concentration organic wastewater treatment method, the high-concentration organic wastewater is distiller's grains leachate, comprising:

[0092] The high-concentration organic wastewater (i.e. distiller's grains leachate) is diluted and sterilized to obtain a to-be-treated liquid;

[0093] The compound microbial inoculant in the first aspect of the present application is inoculated into the to-be-treated liquid to form a treatment liquid;

[0094] The treatment liquid is cultured at 30-37°C for 60-80 hours.

[0095] In some optional embodiments of the third aspect of the present application, the treatment liquid is incubated at 37°C for 72 hours.

[0096] In some examples, the high-concentration organic wastewater treatment method provided by the third aspect of the present application uses bioaugmentation technology to realize decontamination treatment of distiller's grains leachate, reduces the organic pollutant content of the distiller's grains leachate, and adjusts the pH value of the distiller's grains leachate.

Claims

1. Application of a complex microbial agent in the decontamination treatment of distiller's spent grain leachate, characterized in that, The complex microbial agent is used for degrading COD of the leachate of distiller's grains, degrading NH3-N of the leachate of distiller's grains and increasing pH value of the leachate of distiller's grains, The active components of the complex microbial agent include two or three of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain, The Paecilomyces varioti MM2 strain is preserved in China General Microbiological Culture Collection Center on July 8, 2013, and the preservation number is CGMCC NO.7903; the Aspergillus fumigatus MM6 strain is preserved in China General Microbiological Culture Collection Center on July 8, 2013, and the preservation number is CGMCC NO.7904; the Candida rugosa MJ4 strain is preserved in China General Microbiological Culture Collection Center on July 8, 2013, and the preservation number is CGMCC NO.7902; and the Bacillus licheniformis MX8 strain is preserved in China General Microbiological Culture Collection Center on July 8, 2013, and the preservation number is CGMCC NO.7901.

2. The application of the complex bacterial agent in the decontamination treatment of lees filtrate according to claim 1, characterized in that, The active components of the complex microbial agent are the Bacillus licheniformis MX8 strain and the Aspergillus fumigatus MM6 strain.

3. The application of the complex bacterial agent in the decontamination treatment of lees filtrate according to claim 1, characterized in that, The active components of the complex microbial agent are two or three strains, and the complex microbial agent includes the Aspergillus fumigatus MM6 strain and the Paecilomyces varioti MM2 strain.

4. The application of the complex bacterial agent in the decontamination treatment of lees filtrate according to claim 1, characterized in that, The active components of the complex microbial agent are the Bacillus licheniformis MX8 strain and the Candida rugosa MJ4 strain.

5. The application of the complex bacterial agent in the decontamination treatment of lees filtrate according to claim 1, characterized in that, The active components of the complex microbial agent are the Bacillus licheniformis MX8 strain, the Candida rugosa MJ4 strain and the Paecilomyces varioti MM2 strain.

6. The application of the complex bacterial agent in the decontamination treatment of lees filtrate according to claim 1, characterized in that, The active components of the complex microbial agent are two or three strains, and the complex microbial agent includes the Aspergillus fumigatus MM6 strain.

7. The application of the complex bacterial agent in the decontamination treatment of lees filtrate according to claim 1, characterized in that, The other strains remaining after removing the strains included in the complex microbial agent from the four strains of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain are also added to the wine lees filtrate for decontamination.

8. The application of the complex microbial agent in the decontamination treatment of lees filtrate according to claim 7, characterized in that, The ratio of colony forming units of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain in the wine lees filtrate treatment process is (7.55-8) : (158-159) : 1 : (1141-1142).

9. A method for treating high concentration organic wastewater, characterized by, The high-concentration organic wastewater is wine lees filtrate, which comprises: The high-concentration organic wastewater is diluted and sterilized to obtain a to-be-treated liquid; The complex microbial agent as claimed in claim 1 is inoculated into the to-be-treated liquid to form a treatment liquid; The treatment liquid is cultured at 30-37°C for 60-80 hours, and the complex microbial agent is used for simultaneously degrading COD of wine lees filtrate, degrading NH3-N of wine lees filtrate and increasing pH value of wine lees filtrate.

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