A method for collecting and preparing complete body specimens of micro-insects

Abstract

The present invention discloses a method for collecting and preparing complete body specimens of micro-sized insects, which belongs to the technical field of entomological specimen preparation, and specifically relates to a method for collecting complete body specimens of micro-sized insects. The purpose is to provide complete insect body materials for preparing high-quality specimens. The technical scheme consists of two parts: (1) trapping of micro-sized insects and (2) collection of complete bodies of micro-sized insects. Insect trapping color plates or insect pheromone traps are used to trap insects. In the laboratory, the insect body is cut together with the insect trapping color plate according to the approximate outline of the insect body to be prepared as a specimen. After being soaked in anhydrous ethanol for 20 to 30 minutes, the insect body and the insect trapping color plate are peeled off under a dissecting microscope. The peeled insect body is placed on filter paper, and the posture and wings are adjusted to allow the insect body to dry quickly. According to the above method, the complete body of the micro-sized insect can be collected, providing complete insect body materials for preparing high-quality specimens.

Description

Technical Field

[0001] The invention belongs to the technical field of entomological specimen preparation, and in particular relates to a method for collecting and preparing complete body specimens of micro-sized insects. Background Art

[0002] Insect body collection is one of the important links in insect specimen preparation. Traditional methods include catching with a capture net, light trapping, food trapping, shaking and catching, manual search and capture, etc. However, the above methods are not applicable to micro-insects with a body length of less than 10 mm or cannot collect the complete body of micro-insects, such as green stink bugs, leafhoppers, whiteflies, golden moths, pear borers, apple borers and other insects. Summary of the invention

[0003] The technical problem to be solved by the present invention is to provide a method for collecting the complete bodies of micro-insects in response to the deficiencies in the existing insect collection technology, with the purpose of collecting the complete bodies of insects for making micro-insect specimens.

[0004] In order to solve the technical problem of the present invention, the following technical solution is adopted:

[0005] A method for collecting and preparing complete body specimens of micro-insects, wherein the characteristic steps are as follows:

[0006] 1. Trapping of micro-insects

[0007] There are two methods for trapping microscopic insects.

[0008] 1.1 Using the insect's color tendency, hang the insect trapping color plate in the field, vegetable shed or orchard, peel off the anti-sticking protective film on the insect trapping color plate, and gently stick the anti-sticking protective film back to the insect trapping color plate after one week, bring it back to the laboratory for use, and replace it with a new insect trapping color plate. The insect trapping color plate is a traditional rosin pressure-sensitive color plate, suitable for outdoor use.

[0009] 1.2 Using the attraction of male insects to female pheromones, hang triangular or boat-shaped insect pheromone traps in fields, vegetable sheds or orchards. Use the sex attractant core and insect trapping color plate to continuously and slowly release pheromones that imitate the courtship of female insects to attract male insects for trapping. After one week, remove the insect trapping color plate in the triangle or boat shape, bring it back to the laboratory for standby use, and replace it with a new sex attractant core and insect trapping color plate.

[0010] 2. Collection of complete bodies of micro-insects

[0011] According to the general outline of the insect body to be made into a specimen, cut off the insect body together with the insect trap color plate, place it in a culture dish filled with anhydrous ethanol, immerse the insect body in anhydrous ethanol, soak for 20 to 30 minutes, place the culture dish under a dissecting microscope, and use a dissecting needle and straight-headed dissecting forceps to peel off the insect body and the insect trap color plate. The peeling order is: antennae-appendages-wings-head-thorax-abdomen.

[0012] The insect body peeled off from the insect trap color plate is gently picked up with tweezers, placed on filter paper, and adjusted and wings spread. The insect body is quickly dried through absorption by the filter paper and volatilization of ethanol.

[0013] The complete bodies of microscopic insects can be collected by the above method, providing complete insect body materials for making high-quality specimens. DETAILED DESCRIPTION

[0014] Prepare insect trapping tools such as insect trapping color plates or insect pheromone traps, and prepare insect collection tools such as anhydrous ethanol, culture dishes, dissecting microscopes, dissecting needles, dissecting tweezers, and filter paper.

[0015] 1. Trapping of micro-insects

[0016] There are two methods for trapping microscopic insects.

[0017] 1.1 Choose a clear and windless day, use the insects' color tendency, hang the insect trapping color plate in the field, vegetable shed or orchard, peel off the anti-sticking protective film on the insect trapping color plate, and gently stick the anti-sticking protective film back to the insect trapping color plate after one week, bring it back to the laboratory for use, and replace it with a new insect trapping color plate. The insect trapping color plate is a traditional rosin pressure-sensitive color plate, suitable for outdoor use.

[0018] 1.2 Choose a clear and windless day, hang a triangular or boat-shaped insect pheromone trap in the field, vegetable shed or orchard, and use the sex attractant core and insect trap color plate to continuously and slowly release pheromones that imitate the courtship of female insects to attract male insects for trapping. After one week, remove the insect trap color plate, bring it back to the laboratory for standby use, and replace it with a new insect trap color plate.

[0019] 2. Collection of complete bodies of micro-insects

[0020] In the laboratory, according to the general outline of the insect body to be made into a specimen, cut off the insect body together with the insect trap color plate, and place it in a culture dish filled with anhydrous ethanol. The anhydrous ethanol should immerse the insect body. After soaking for 20 to 30 minutes, place the culture dish under a dissecting microscope and use a dissecting needle and straight-headed dissecting forceps to peel off the insect body and the insect trap color plate. The peeling order is: antennae-appendages-wings-head-thorax-abdomen.

[0021] The insect body peeled off from the insect trap color plate is gently picked up with tweezers, placed on filter paper, and adjusted and wings spread. The insect body is quickly dried through absorption by the filter paper and volatilization of ethanol.

[0022] The complete bodies of microscopic insects can be collected by the above method, providing complete insect body materials for making high-quality specimens.

Claims

1. A method for collecting and preparing complete body specimens of micro-insects, characterized in that The process consists of two parts: (1) trapping micro-insects and (2) collecting the complete bodies of micro-insects. By utilizing the color attraction of insects, an insect trapping color plate is hung in a field, vegetable shed or orchard, and the anti-stick protective film on the insect trapping color plate is peeled off. After one week, the anti-stick protective film of the insect trapping color plate is gently glued back to the color plate, and the plate is taken back to the laboratory for standby use, and a new insect trapping color plate is replaced. The insect trapping color plate is a traditional rosin pressure-sensitive color plate; or by utilizing the attraction of male insects to female pheromones, a triangular or boat-shaped insect pheromone trap is hung in a field, vegetable shed or orchard, and the sex attractant core is combined with the insect trapping color plate to continuously and slowly release pheromones that imitate the courtship of female insects to attract male insects for trapping. After one week, the insect trapping color plate in the triangular or boat shape is removed and taken back to the laboratory for standby use. Return to the laboratory for use and replace with a new insect trapping color plate. Cut out the insect body together with the insect trapping color plate according to the approximate outline of the insect body to be made into specimens, and place them in a culture dish filled with anhydrous ethanol. Immerse the insect body in anhydrous ethanol. After soaking for 20 to 30 minutes, place the culture dish under a dissecting microscope and use a dissecting needle and straight-headed dissecting tweezers to peel off the insect body and the insect trapping color plate. The peeling order is: antennae - appendages - wings - head - thorax - abdomen. Gently pick up the insect body peeled off from the insect trapping color plate with tweezers, place it on filter paper, adjust its posture and spread its wings. The insect body is quickly dried through the absorption of the filter paper and the volatilization of ethanol. The complete body of the micro-insect can be collected according to the above method, providing complete insect material for making high-quality specimens.

2. The method for collecting and preparing complete body specimens of micro-sized insects according to claim 1, characterized in that According to the general outline of the target insect body for specimen preparation, cut out the insect body together with the insect trapping color plate, and soak it in anhydrous ethanol for 20 to 30 minutes.

3. The method for collecting and preparing complete body specimens of micro-sized insects according to claim 1, characterized in that Under a dissecting microscope, use a dissecting needle and straight-tipped dissecting forceps to separate the insect body from the insect trap color plate.

4. The method for collecting and preparing complete body specimens of micro-sized insects according to claim 1, characterized in that The insect body peeled off from the insect trap color plate is gently picked up with tweezers, placed on filter paper, and adjusted and wings spread. The insect body is quickly dried through absorption by the filter paper and volatilization of ethanol.