Use of a fungicidal composition containing prothioconazole and mefenoxam to control diseases of peanuts

Abstract

The present application relates to a kind of fungicidal composition containing prothioconazole and mefenoxam in the use for preventing and treating peanut diseases;The pesticide composition at least includes active ingredient A prothioconazole and active ingredient B mefenoxam, the mass ratio of the active ingredient A prothioconazole and active ingredient B is 1:50-50:1.The fungicidal composition of the present application can be used for preventing and treating various diseases on peanut, has good control effect, can prolong the effective period of pesticide, reduce the frequency of use, effectively reduce the use amount of active ingredient and use cost, delay the generation and development of resistance, and is environment-friendly.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of fungicide application, in particular to the use of a fungicidal composition containing prothioconazole and metalaxyl-M for preventing and treating peanut diseases. BACKGROUND

[0002] Prothioconazole is a sterol demethylation (ergosterol biosynthesis) inhibitor, which is an endoabsorptive fungicide with protective, curative and eradicative activity, and has a long persistence. It is mainly used for preventing and treating many diseases of cereal, wheat, legume crops, etc. Prothioconazole has low toxicity, no teratogenicity, no mutagenicity, and no embryo toxicity, and is safe to human and environment when used correctly. The CAS registration number is 178928-70-6, and its chemical structural formula is:

[0003]

[0004] Metalaxyl-M is an endoabsorptive aniline compound, which has very good prevention and treatment effect on many seed-borne and soil-borne diseases caused by lower fungi such as Pythium and Saprolegnia. It is an endoabsorptive fungicide absorbed by plant leaves, stems and roots, and has protective and curative activity. It inhibits protein synthesis of fungi by blocking ribonucleic acid synthesis. The CAS registration number is 70630-17-0, and its chemical structural formula is:

[0005]

[0006] Peanut is an important oil crop and is planted in all parts of the country. In recent years, with the steady increase of economic benefits of planting peanuts, the planting area has been increasing, causing difficulty in crop rotation and year-round continuous cropping. At the same time, due to the continuous improvement of production level, the growth of peanuts is vigorous, the field ventilation and light penetration are poor, and the humidity is large, which provides suitable ecological environment conditions for the occurrence of peanut root and stem diseases. In addition, the rural labor force is decreasing, the field management is extensive, and the weeds are rampant, which leads to the increasing damage of peanut root and stem diseases year by year, resulting in low yield, poor quality and poor economic benefits of peanuts.

[0007] With the long-term use of a single selective pesticide, different degrees of drug resistance have appeared in the pathogenic fungal population in nature, and the prevention and treatment effect has decreased year by year. Chemical control is one of the effective measures to control soil-borne fungal diseases of plants, and seed pesticide coating saves time and labor, and is more easily accepted by farmers.

[0008] Patent application with publication number CN105394048A discloses a fungicidal composition containing propiconazole and high-efficiency metalaxyl-M. The effective components of the pesticide composition are propiconazole and high-efficiency metalaxyl-M, which can effectively control diseases such as gray mold, powdery mildew and rust on vegetables, flowers and fruits. With the increasing requirements of environment and food safety, and the problem of pesticide resistance, how to use pesticides scientifically, reduce the amount of chemical pesticides and improve the efficacy has become a problem urgently to be solved in the field of pesticides. In addition, there is no related report on the use of the fungicidal composition containing propiconazole and metalaxyl-M in preventing and treating peanut diseases. The general farmers have a deeper understanding of environmental protection concept, and high efficiency, low toxicity, high activity and low residue have become an inevitable trend of pesticide development. SUMMARY

[0009] Based on the above, the purpose of the present application is to provide a use of a fungicidal composition containing propiconazole and metalaxyl-M in preventing and treating peanut diseases. The fungicidal composition can be used to prevent and treat various diseases on peanuts, has good quick-acting property and can delay the development of peanut disease resistance.

[0010] To achieve the above purpose, the use of a fungicidal composition containing propiconazole and metalaxyl-M in preventing and treating peanut diseases, wherein the fungicidal composition at least comprises active ingredient A and active ingredient B, the active ingredient A is propiconazole, the active ingredient B is metalaxyl-M, and the mass ratio of the active ingredient A propiconazole to the active ingredient B metalaxyl-M is 50:1-1:50.

[0011] Further, the mass ratio of the active ingredient A propiconazole to the active ingredient B metalaxyl-M is 10:1-1:10.

[0012] Further, the mass ratio of the active ingredient A propiconazole to the active ingredient B metalaxyl-M is 10:1, 7:1, 3:1, 2:1, 5:3, 3:2, 1:1, 3:4, 1:5 or 1:10.

[0013] Further, the mass ratio of the active ingredient A propiconazole to the active ingredient B metalaxyl-M is 7:1-1:5.

[0014] Further, the mass ratio of the active ingredient A propiconazole to the active ingredient B metalaxyl-M is 3:1-3:4.

[0015] Further, the mass ratio of the active ingredient A propiconazole to the active ingredient B metalaxyl-M is 3:1, 2:1, 5:3, 3:2, 1:1 or 3:4.

[0016] Further, the total content of the active ingredient A and the active ingredient B in the fungicidal composition is 0.1%-80% based on 100wt% of the total weight of the fungicidal composition.

[0017] Further, the sum of the content of the active ingredient A and the active ingredient B in the fungicidal composition is 2% to 50% based on 100% by weight of the total weight of the fungicidal composition;

[0018] Further, the sum of the content of the active ingredient A and the active ingredient B in the fungicidal composition is 2% to 30% based on 100% by weight of the total weight of the fungicidal composition;

[0019] Further, the fungicidal composition further comprises an adjuvant selected from one or more of wetting agents, dispersants, emulsifiers, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, warning colors, film formers, synergists, and carriers;

[0020] Further, the dosage form of the fungicidal composition is any one of the agriculturally permissible dosage forms;

[0021] Further, the dosage form of the fungicidal composition is any one of aqueous solution, wettable powder, powder, microemulsion, emulsion, suspension, emulsifiable concentrate, water dispersible granule, suspension seed coating agent, seed treatment suspension, microcapsule suspension, and dry seed dressing agent;

[0022] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl sulfosuccinate sodium, alpha-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sulfate sodium, silkworm excrement, soap nut, soapberry, SOPA, detergent, emulsifier 2000 series, and wetting penetrant F; and / or

[0023] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylate, phosphate, EO-PO block copolymer, and EO-PO graft copolymer; and / or

[0024] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0025] The thickening agent is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and white carbon black; and / or

[0026] Disintegrant The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0027] Antifreezing agent The antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0028] Defoaming agent The defoaming agent is selected from one or more of C 10 -C 20 Saturated fatty acid compound, silicone oil, silicone compound, C8-C 10 Fatty alcohol; and / or

[0029] Solvent The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent oil, vegetable oil, vegetable oil derivative, and water; and / or

[0030] Preservative The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, carboxin, and 1,2-benzisothiazolin-3-one; and / or

[0031] Stabilizer The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon, talc, montmorillonite, and starch; and / or

[0032] Warning color The warning color is selected from one or more of a regulated color of blue, green, red, and purple; and / or

[0033] Film former The film former is selected from one or more of sodium carboxymethyl starch, cellulose derivative (sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol), polyacrylic acid; and / or

[0034] Further, the film former is selected from seed coating film former 851, seed coating film former 805; and / or

[0035] Synergist The synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0036] Carrier The carrier is selected from one or more of ammonium salt, ground natural mineral, ground artificial mineral, silicate, resin, wax, solid fertilizer, water, organic solvent, mineral oil, vegetable oil, and vegetable oil derivative;

[0037] Further, the dosage form of the fungicidal composition is a seed treatment suspension concentrate.

[0038] Use of a fungicidal composition containing ipconazole and mefenoxam for controlling peanut diseases, the peanut diseases being bacterial diseases or fungal diseases;

[0039] Further, the peanut diseases are any one or more of Cercospora personata, Cercospora arachidicola, Ascochyta arachidis, Colletotrichum truncatum, Leptosphaerulina crassiasca, Helicobasidium mompa, Puccinia arachidis, Balstona solanacearum, Diplodia gossypina, Sclerotium rolfsii, peanut root rot, Cucumber Mosaic Virus-China arachis (CMV-CA), Peanut stripe Virus (PStV), Peanut stunt Virus (PSV), and Aspergillus niger.

[0040] Further, the peanut diseases are peanut root rot and Sclerotium rolfsii.

[0041] The peanut root rot is caused by Sclerotium rolfsii, a dematiaceous fungus. It produces both basidia and basidiospores, but they are not common. The mycelium is white, and often several to tens of twisted threads radiate outward, appearing as white silk, hence the common name of white thread disease. Peanut root rot mainly damages the stems, fruit stalks and pods of peanuts. At the early stage of the disease, the diseased part turns brown and softens, and wave-shaped lesions appear on it. When the soil is moist and hidden, the soil surface around the diseased plants and organic matter is also covered with a layer of white mycelium. Many spherical sclerotia are formed in the mycelium. The base of the affected stem tissue rots, the cortex peels off, and only fibrous tissue is left. The leaves of the diseased plant turn yellow, the edges are scorched, and finally wither and die. The fruit stalks and pods of the affected plants grow many white mycelia, which appear as wet and rotten.

[0042] Further, the peanut diseases are peanut root rot.

[0043] The peanut root rot is caused by a plurality of fusarium and pythium, and fusarium oxysporum and pythium myriotylum are two main pathogenic bacteria. Among them, the fusarium app of the pathogenic fungus causing peanut root rot includes fusarium oxysporum, fusarium solani, fusarium roseum, fusarium tricinctum and fusarium moniliforme. The peanut root rot can occur in each growth period, and mainly harms the roots of the plants. The pathogenic bacteria infect the just germinated seeds, causing rotten seeds; the seedlings are harmed, the main roots become brown, and the plants wither. The adult plants are harmed, the main roots appear concave long strip brown disease spots, the root end is wet and rotten, the cortex is brown and rotten, easy to separate and fall off, and there are few lateral roots or no lateral roots, which looks like a mouse tail. The adventitious roots are generated at the root and stem part in wet conditions. The aboveground part of the diseased plants is small, grows poorly, the leaves turn yellow, and there are few flowers, fruits and unripe fruits.

[0044] The use of a fungicidal mixture containing prothioconazole and mefenoxam for controlling peanut diseases, the fungicidal composition is applied to the diseases in need of control or the growth medium thereof at an effective dose.

[0045] Compared with the prior art, the beneficial effects of the technical scheme of the present application are as follows:

[0046] 1) The fungicidal composition of the present application can prevent and control peanut diseases, expand the control spectrum, save labor and drug cost;

[0047] 2) The fungicidal composition of the present application shows a certain synergistic effect on the prevention and control of peanut diseases within a certain range, can reduce the use amount of active ingredients of pesticides, and improve the prevention and control effect;

[0048] 3) The fungicidal composition of the present application can prolong the persistence period of the pesticide, reduce the number of pesticide applications, delay the development of pest resistance, and prolong the service life of the single agent. DETAILED DESCRIPTION

[0049] In order to make the technical scheme, purpose and advantages of the present application clearer and more apparent, the present application is described by the following specific examples, but the present application can be realized in various forms and should not be limited by the embodiments described herein.

[0050] Laboratory bioassay

[0051] The example refers to the pesticide laboratory bioassay test standard, part 2, inhibition of pathogenic fungal mycelium growth plate method, NY / T 1156.2-2006.

[0052] Experimental instruments and equipment: electronic balance (sensitivity 0.01 mg), super-clean workbench, biological incubator, Φ9 cm culture dish, pipette, inoculation loop, puncher, caliper, etc.

[0053] Test agents: 95% prothioconazole technical material and 91% metalaxyl technical material were provided by the Group R&D Center.

[0054] Example 1 in the laboratory

[0055] Laboratory determination of the virulence of peanut white thread blight

[0056] Test pathogen: Sclerotium rolfsii Sacc, provided by the Group R&D Center.

[0057] Agent preparation: prothioconazole and metalaxyl technical materials were first dissolved in acetone, and then diluted with 0.1% Tween-80 aqueous solution. Single-agent stock solutions were prepared, and 5 groups of mixtures were prepared according to the mixing purpose and the activity of the agents. Each single agent and each group of mixture was prepared in 5 series of concentrations by the method of equal proportion.

[0058] Under sterile operating conditions, 45 ml of pre-melted sterilized culture medium was added to a sterile conical flask according to the test treatment. From low concentration to high concentration, 5 ml of drug solution was taken and added to the above-mentioned conical flask, shaken well, and then poured into 3 culture dishes in equal amounts to prepare drug-containing plates of the corresponding concentration. The test set up a blank control without agents, and each treatment was repeated 3 times.

[0059] The cultured pathogen was cut into a cake with a sterilized puncher with a diameter of 5 mm from the edge of the colony under sterile operating conditions. The cake was inoculated in the center of the drug-containing plate with the mycelium facing up using an inoculator. One cake was placed in each culture dish, covered with a dish cover, and placed in a (26±0.5℃) incubator for culture.

[0060] Data statistics and analysis:

[0061] According to the growth of the fungus in the blank control culture dish, the mycelial growth of the pathogen was investigated. The diameter of the colony was measured with a caliper, and the unit was millimeter (mm). Each colony was measured vertically with the cross method once, and the average value was taken.

[0062] According to the investigation results, the mycelial growth inhibition rate of each treatment concentration on the test target fungus was calculated, and the unit was percentage (%). The calculation results were rounded to two decimal places.

[0063] D = D1 - D2

[0064] In the formula:

[0065] D - colony growth diameter;

[0066] D1 - colony diameter;

[0067] D2 - diameter of the colony.

[0068]

[0069] In the formula:

[0070] I - mycelium growth inhibition rate;

[0071] D0 - diameter of the blank control colony growth;

[0072] D T - diameter of the colony treated with the agent.

[0073] According to the numerical values of each agent concentration and the corresponding mycelium growth inhibition rate probability values, regression analysis was performed to obtain the EC 50 value and correlation coefficient r of the toxicity regression line.

[0074] Sun Yunpei method: the synergistic effect of mixed agents was evaluated according to the co-toxicity coefficient (CTC). When the CTC of the mixed preparation was ≥120, it was considered to have a synergistic effect; when the CTC was ≤80, it was considered to have an antagonistic effect; and when 80 < CTC < 120, it was considered to have an additive effect.

[0075] Calculation of the co-toxicity coefficient (CTC value) of the mixed agent:

[0076]

[0077] In the formula:

[0078] ATI - measured toxicity index of the mixed agent;

[0079] S - EC 50 of the standard fungicide, in milligrams per liter (mg / L);

[0080] M - EC 50 of the mixed agent, in milligrams per liter (mg / L).

[0081] TTI = TIA * PA + TI B * P B

[0082] In the formula:

[0083] TTI - theoretical toxicity index of the mixed agent;

[0084] TI A - toxicity index of agent A;

[0085] P A - percentage content of agent A in the mixed agent, in percentage (%);

[0086] TI B— B agent toxicity index;

[0087] P B — Percentage of B agent in the mixture, in percentage (%).

[0088]

[0089] In the formula:

[0090] CTC - Co-toxicity coefficient;

[0091] ATI - Actual toxicity index of the mixture;

[0092] TTI - Theoretical toxicity index of the mixture.

[0093] Results and analysis:

[0094] The two agents, propiconazole and mefenoxam, have different mechanisms of action, and their combined use is beneficial to overcoming or delaying the development of drug resistance of the pathogen and improving the control effect.

[0095] The test results show that, as shown in Table 1, both propiconazole single agent and propiconazole and mefenoxam complex preparation have good control effect on peanut Sclerotium blight fungus, and the EC 50 of propiconazole EC is 0.798 mg / L, and propiconazole and mefenoxam in the range of 10:1 to 1:10 show synergistic effect, among which the mass ratio of propiconazole to mefenoxam is 3:2, the EC 50 is 0.547 mg / L, and the co-toxicity coefficient is 145.887.

[0096] Table 1 Indoor biological test results of propiconazole, mefenoxam and their complex mixture on peanut Sclerotium blight fungus

[0097]

[0098] Note: 1. The concentration in the propiconazole and mefenoxam mixture refers to the concentration of propiconazole;

[0099] 2. Mefenoxam has no obvious inhibitory activity on peanut Sclerotium blight fungus at the designed highest concentration of 200 mg / L, and cannot calculate EC 50 and toxicity regression equation.

[0100] Indoor Example 2

[0101] Peanut root rot indoor toxicity test

[0102] Test pathogen: Pythium myriotylum, Fusarium oxysporum, provided by the Group R&D Center.

[0103] Formulation of the pesticides: The prothioconazole and mefenoxam technical were dissolved in acetone, and then diluted with 0.1% Tween-80 aqueous solution. The single agent stock solutions were prepared, and five groups of mixtures were prepared according to the mixing purpose and the activity of the pesticides. Five series of concentrations were prepared for each single agent and each group of mixtures by the method of equal ratio.

[0104] Under sterile operation conditions, 45 ml of sterilized culture medium was added into a sterile conical flask according to the test treatment. 5 ml of pesticide solution was taken from low concentration to high concentration and added into the conical flask, which was shaken well and then poured into three culture dishes in equal amounts to prepare the corresponding concentration of drug-containing plates. The treatment without pesticide was used as a blank control, and each treatment was repeated three times.

[0105] The cultured pathogenic bacteria were cut into 5 mm diameter disks from the edge of the colony under sterile operation conditions. The disks were inoculated in the center of the drug-containing plates using an inoculator with the mycelium facing up. One disk was placed in each culture dish, which was covered with a lid and placed in a (26±0.5℃) incubator for culture.

[0106] Data statistics and analysis:

[0107] According to the growth of the bacteria in the blank control culture dishes, the mycelial growth of the pathogenic bacteria was investigated. The diameter of the colony was measured using a caliper, and the unit was millimeter (mm). Each colony was measured vertically once using the cross method, and the average value was taken.

[0108] According to the investigation results, the mycelial growth inhibition rate of each treatment concentration on the test target bacteria was calculated, and the unit was percentage (%). The calculation results were rounded to two decimal places.

[0109] D=D1-D2

[0110] In the formula:

[0111] D —— the diameter of the colony growth;

[0112] D1 —— the diameter of the colony;

[0113] D2 —— the diameter of the disk.

[0114]

[0115] In the formula:

[0116] I —— the mycelial growth inhibition rate;

[0117] D0 —— the diameter of the colony growth in the blank control;

[0118] D T —— the diameter of the colony growth treated with pesticides.

[0119] The logarithm of each concentration of the agent and the corresponding mycelium growth inhibition rate probability value were used for regression analysis. The EC value and correlation coefficient r of the toxicity regression line were calculated. 50

[0120] Sun Yunpei method: The synergistic effect of mixed agents was evaluated according to the co-toxicity coefficient (CTC). When the CTC of the mixed preparation was ≥120, it showed synergistic effect; when the CTC was ≤80, it showed antagonistic effect; and when 80 < CTC < 120, it showed additive effect.

[0121] Calculation of the co-toxicity coefficient (CTC value) of the mixed agent:

[0122]

[0123] In the formula:

[0124] ATI - measured toxicity index of the mixed agent;

[0125] S - EC of the standard fungicide, unit: milligrams per liter (mg / L); 50

[0126] M - EC of the mixed agent, unit: milligrams per liter (mg / L). 50

[0127] TTI = TI A * P A + TI B * P B

[0128] In the formula:

[0129] TTI - theoretical toxicity index of the mixed agent;

[0130] TI A - toxicity index of agent A;

[0131] P A - percentage content of agent A in the mixed agent, unit: percentage (%);

[0132] TI B - toxicity index of agent B;

[0133] P B - percentage content of agent B in the mixed agent, unit: percentage (%).

[0134]

[0135] In the formula:

[0136] CTC - co-toxicity coefficient;

[0137] ATI - measured toxicity index of the mixed agent; ​​​

[0138] TTI - Theoretical Toxicity Index of the mixture.

[0139] Results and analysis:

[0140] The two agents have different mechanisms of action, and the use of a mixture can help overcome or delay the development of drug resistance and improve control effect.

[0141] The test results show that, as shown in Table 2, the two single agents and their mixed preparations have good control effect on peanut root rot fungus (Pythium aphanidermatum). The EC50 of propiconazole EC is 2.474 mg / L, and the EC50 of mefenoxam EC is 0.364 mg / L. Propiconazole and mefenoxam 7:1-1:5 all show synergistic effect, among which propiconazole: mefenoxam = 3:2 has better activity, and the coefficient of joint toxicity is 215.456. 50 50

[0142] Table 2 Indoor bioassay results of propiconazole, mefenoxam and their mixed preparations on peanut root rot fungus (Pythium aphanidermatum)

[0143]

[0144] The test results show that, as shown in Table 3, propiconazole single agent and its mixed preparation with mefenoxam have good control effect on peanut root rot fungus (Fusarium oxysporum). The EC50 of propiconazole EC is 1.149 mg / L. Propiconazole and mefenoxam 7:1-1:5 all show synergistic effect, among which propiconazole: mefenoxam = 3:2 has better activity, and the coefficient of joint toxicity is 152.590. 50

[0145] Table 3 Indoor bioassay results of propiconazole, mefenoxam and their mixed preparations on peanut root rot fungus (Fusarium oxysporum)

[0146]

[0147] Note: 1. The concentration in the propiconazole: mefenoxam mixture refers to the concentration of propiconazole;

[0148] 2. Mefenoxam has no obvious inhibitory activity on peanut root rot fungus (Fusarium oxysporum) at the designed highest concentration of 200 mg / L, and the EC50 and toxicity regression equation cannot be calculated. 50

[0149] Preparation Example 1

[0150] 20% propiconazole · mefenoxam seed treatment suspension (10+10)

[0151] ​​​​Disperse alkyl naphthalene sulfonate formaldehyde condensate 3%, EO-PO block copolymer 1%, sodium lauryl sulfate 2% and silicone antifoaming agent 0.5% in an appropriate amount of water, magnesium aluminum silicate 0.5%, propylene glycol 4%, warning color 5%, disperse prothioconazole 15% and mefenoxam 5% therein, use zirconium oxide beads, perform wet milling to D 90 (90% of the particle size of the particles) <5 μm, to obtain a pulverized slurry. Add magnesium aluminum silicate 0.1%, sodium p-hydroxybenzoate 0.6%, polyacrylic acid (805) 5% to the pulverized slurry and mix uniformly, deionized water to make up to 100%, uniformly prepared at high speed shear to obtain a seed treatment suspension with an active ingredient mass content of 20 wt%.

[0152] Preparation Example 2

[0153] 15% prothioconazole · mefenoxam seed treatment suspension (9+6)

[0154] Disperse alkyl naphthalene sulfonate formaldehyde condensate 3%, EO-PO block copolymer 1%, sodium lauryl sulfate 2% and silicone antifoaming agent 0.5% in an appropriate amount of water, magnesium aluminum silicate 0.5%, propylene glycol 4%, warning color 5%, disperse prothioconazole 15% and mefenoxam 5% therein, use zirconium oxide beads, perform wet milling to D 90 (90% of the particle size of the particles) <5 μm, to obtain a pulverized slurry. Add magnesium aluminum silicate 0.1%, sodium p-hydroxybenzoate 0.6%, polyacrylic acid (805) 5% to the pulverized slurry and mix uniformly, deionized water to make up to 100%, uniformly prepared at high speed shear to obtain a seed treatment suspension with an active ingredient mass content of 20 wt%.

[0155] Preparation Example 3

[0156] 20% prothioconazole · mefenoxam seed treatment suspension (15+5)

[0157] Disperse alkyl naphthalene sulfonate formaldehyde condensate 3%, EO-PO block copolymer 1%, sodium lauryl sulfate 2% and silicone antifoaming agent 0.5% in an appropriate amount of water, magnesium aluminum silicate 0.5%, propylene glycol 4%, warning color 5%, disperse prothioconazole 15% and mefenoxam 5% therein, use zirconium oxide beads, perform wet milling to D 90 (90% of the particle size of the particles) <5 μm, to obtain a pulverized slurry. Add magnesium aluminum silicate 0.1%, sodium p-hydroxybenzoate 0.6%, polyacrylic acid (805) 5% to the pulverized slurry and mix uniformly, deionized water to make up to 100%, uniformly prepared at high speed shear to obtain a seed treatment suspension with an active ingredient mass content of 20 wt%.

[0158] Preparation Example 4

[0159] 26% prothioconazole + mefenoxam seed treatment suspension concentrate (14 + 12)

[0160] Disperse lignosulfonate sodium 1.5%, polycarboxylate 1.5%, sodium dodecyl sulfate 2% and silicone antifoaming agent 0.5% in appropriate amount of water, magnesium aluminum silicate 0.5%, propylene glycol 4%, warning color 5%, disperse prothioconazole 14% and mefenoxam 12% therein, use zirconium oxide beads, wet grinding with sand mill to D 90 (90% of the particle size of the particles) <5 μm, to obtain the crushing slurry. Add xanthan gum 0.1%, sodium benzoate 0.5%, polyacrylic acid (805) 5% to the crushing slurry and mix uniformly, deionized water to 100%, high-speed shear uniformly to prepare a seed treatment suspension concentrate with a mass content of 26% of the active ingredients.

[0161] Preparation Example 5

[0162] 30% prothioconazole + mefenoxam seed treatment suspension concentrate (20 + 10)

[0163] Disperse lignosulfonate sodium 2.5%, polycarboxylate 1%, sodium dodecyl sulfate 1.5% and silicone antifoaming agent 0.5% in appropriate amount of water, magnesium aluminum silicate 0.5%, propylene glycol 5%, warning color 5%, disperse prothioconazole 20% and mefenoxam 10% therein, use zirconium oxide beads, wet grinding with sand mill to D 90 (90% of the particle size of the particles) <5 μm, to obtain the crushing slurry. Add xanthan gum 0.1%, sodium benzoate 0.6%, polyacrylic acid (805) 5% to the crushing slurry and mix uniformly, deionized water to 100%, high-speed shear uniformly to prepare a seed treatment suspension concentrate with a mass content of 30% of the active ingredients.

[0164] Preparation Example 6

[0165] 28% flubendiamide + prothioconazole + mefenoxam seed treatment suspension concentrate (16 + 12)

[0166] Disperse lignosulfonate sodium 2.4%, EO-PO block copolymer 1.5%, sodium dodecyl sulfate 1.5% and silicone antifoaming agent 0.5% in appropriate amount of water, magnesium aluminum silicate 0.5%, propylene glycol 4%, warning color 5%, disperse prothioconazole 16% and mefenoxam 12% therein, use zirconium oxide beads, wet grinding with sand mill to D 90 (90% of the particle size of the particles) <5 μm, to obtain the crushing slurry. Add xanthan gum 0.1%, sodium benzoate 0.5%, polyacrylic acid (805) 5% to the crushing slurry and mix uniformly, deionized water to 100%, high-speed shear uniformly to prepare a seed treatment suspension concentrate with a mass content of 28% of the active ingredients. Specific Examples

[0168] In China, in recent years, due to the change of cropping system, the peanut in some peanut production areas is continuously planted for many years, and the peanut variety is single, and other factors, the peanut white thread disease and root rot disease are getting worse year by year, and the occurrence area is expanding, especially in Guangxi, Hubei, Shandong, Tianjin, Sichuan and Liaoning, etc. Peanut production areas, white thread disease and root rot disease occur every year.

[0169] Field example 1

[0170] Field efficacy test of peanut white thread disease

[0171] Test object: peanut white thread disease, the pathogen of which is Sclerotium rolfsii Sacc, caused by semi-knowledge fungi.

[0172] Test crop: peanut (Fu Hua No. 8);

[0173] Peanut planting specifications: ridge distance 80 cm, ridge surface width 55 cm, ridge row distance 30 cm, hole distance 16.5 cm, peanut density 150,000 holes / hm 2 , 2 seeds per hole, film mulching cultivation.

[0174] Test site: peanut field in Dongmen Town, Fusui County, Chongzuo City, Guangxi.

[0175] Test field irrigation facilities are good, and the cultivation conditions (soil type, water and fertilizer management, planting density and growth period) of the test plots are uniform and consistent, and meet the local scientific agricultural practice (GAP).

[0176] Plot area and repetition: plot area 15 m 2 , repeated 4 times.

[0177] Investigation time and frequency: a total of 2 investigations, the first investigation was 10 days after peanut sowing, and the seedling rate was investigated; the second investigation was 7 days before peanut harvesting to investigate the disease.

[0178] Method of use: according to the use amount of each pesticide, the pesticide and peanut seeds are weighed, the seeds are poured into the seed dressing container the day before sowing, then the pesticide is poured on the peanut seeds, and the seeds are turned over while pouring, so that the seeds are fully mixed and the color of all peanut surfaces is consistent, then the seeds are pushed away and dried, and then sowing can be performed.

[0179] Investigation method: from peanut sowing to harvesting, whether there is phytotoxicity and its symptoms are observed.

[0180] Each plot takes 5 samples on the diagonal line, 20 piles are investigated at each point, 100 piles are investigated in each plot, and in the second investigation, the peanut roots are pulled out pile by pile, the total number of plants and the number of white thread disease at each level are recorded, and the control effect is calculated.

[0181] Classification method:

[0182] 0 grade: no disease;

[0183] 1 grade: only peanut root lesions, 10% incidence;

[0184] 3 grade: peanut stem base produced obvious lesions, white mycelium appeared, 30% incidence;

[0185] 5 grade: peanut stem base blackened, white mycelium was obvious, 50% incidence;

[0186] 7 grade: peanut whole plant root covered with white mycelium, whole plot began to wither, 80% incidence;

[0187] 9 grade: peanut whole plant was completely covered with white mycelium, root completely blackened and died.

[0188] Pharmacodynamic calculation method:

[0189] Emergence rate (%) = Emergence number / seeded number x 100

[0190] Disease index (%) =∑(each grade diseased plant number x relative grade value) / (total plant number surveyed x highest grade number) x 100

[0191] Control effect (%) = (disease index of control area-disease index of treatment area) / disease index of control area x 100

[0192] From peanut sowing to harvesting, the field growth and appearance of peanut were observed irregularly, and no obvious phytotoxicity symptoms were found.

[0193] The control effect is shown in Table 4, and the mixed preparation shows obvious control effect on peanut Sclerotium blight. Compared with the blank control agent treatment, the incidence index is reduced, and has better control effect.

[0194] Table 4 Control effect of test agent on peanut Sclerotium blight

[0195]

[0196] Note: The control effect (%) in the above table is the average value of each repetition; capital letters represent 1% level significant difference.

[0197] Field example 2

[0198] Peanut root rot field efficacy test

[0199] Test object: peanut root rot, the main pathogenic bacteria causing the disease are Fusarium solani (Mart) Sacc and Fusarium oxysporum Schlecht.

[0200] Test crop: peanut (Fu Hua 8#)

[0201] Peanut planting specifications: ridge distance 80 cm, ridge surface width 55 cm, ridge row distance 30 cm, hole distance 16.5 cm, peanut density 150,000 holes / hm 2 2 seeds per hole, film mulching cultivation.

[0202] Test site: peanut field in Dongmen Town, Fusui County, Chongzuo City, Guangxi. The test field has good irrigation facilities, and the cultivation conditions (soil type, water and fertilizer management, planting density, and growth period) of the test plots are uniform and consistent, and meet the local scientific agricultural practices (GAP).

[0203] Plot area and repetition: plot area 15 m 2 , repeated 4 times.

[0204] Investigation time and frequency: a total of 2 investigations, the first investigation was 10 days after peanut sowing, and the seedling rate was investigated; the second investigation was 60 days after the peanut seedlings were uniform, and the disease was investigated.

[0205] Method of use: according to the use amount of each pesticide, weigh the pesticide and peanut seeds, one day before sowing, pour the seeds into the seed dressing container, then pour the pesticide on the peanut seeds, pour and stir at the same time, fully mix, make all the peanut surfaces uniform in color, then push away and dry, then sow.

[0206] Investigation method: from peanut sowing to harvesting, observe whether there is pesticide damage and its symptoms.

[0207] Each plot takes 5 points on the diagonal line, 20 stakes are investigated at each point, 100 stakes are investigated in each plot, in the second investigation, the peanut roots are pulled out one by one, the total number of plants and the number of root rot disease at each level are recorded, and the control effect is calculated.

[0208] Classification method:

[0209] 0 level: no disease;

[0210] 1 level: root system slightly discolored, discolored root system accounts for less than 10% of the total root system, and the plant is not wilting;

[0211] 3 level: root system is obviously brown, discolored root system accounts for 10.1% to 30% of the total root system, and the plant starts to wilt;

[0212] 5 level: discolored root system accounts for 30.1% to 50% of the total root system, and the plant is obviously wilting;

[0213] 7 level: discolored root system accounts for 50.1% to 80% of the total root system, and the plant is wilting;

[0214] 9 level: whole plant is dead.

[0215] Pesticide efficacy calculation method:

[0216] Emergence rate (%) = Emergence number / sowing number x 100

[0217] Disease index (%) =∑(number of each level of diseased plants x relative level value) / (total number of plants surveyed x highest level) x 100

[0218] Control effect (%) = (disease index of the control area - disease index of the treatment area) / disease index of the control area x 100

[0219] From sowing to harvesting, the growth and appearance of peanuts in the field were observed irregularly, and no obvious phytotoxicity symptoms were found.

[0220] The control effect is shown in Table 5, and the binary mixed pesticide shows obvious control effect on peanut root rot. Compared with the blank control pesticide treatment, the disease index is reduced, and the control effect is better.

[0221] Table 5 Control effect of the test pesticide on peanut root rot

[0222]

[0223] Note: The control effect (%) in the above table is the average value of each repetition; capital letters represent 1% level of significant difference.

[0224] Through indoor toxicity determination and field test, the propiconazole and metalaxyl-M in the present application show good control effect on peanut white thread disease and peanut root rot. The pesticide composition or its preparation obtained by compounding in the present application has significant control effect, is better than single agent in delaying the generation of drug resistance and prolonging the drug resistance, and simultaneously includes the seed treatment suspension of active ingredients propiconazole and metalaxyl-M, which makes the seed coating film fast, does not off-grain, is safe to crop seeds, and has good tolerance. And no phytotoxicity of the compounded pesticide to crops is found in the test, which shows that in the case of synergistic effect of the obtained pesticide composition or preparation in killing bacteria, the production cost and use cost can be reduced, and the crops are safe.

[0225] Although the present application has been described in detail above with general description and specific embodiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art, and therefore, these modifications or improvements made without deviating from the spirit of the present application all belong to the scope of the present application.

Claims

1. Use of a fungicidal composition containing ipconazole and mefenoxam for controlling white mold of peanuts, characterized in that, The fungicidal composition consists of active ingredient A prothioconazole and active ingredient B mefenoxam, and the mass ratio of the active ingredient A prothioconazole to the active ingredient B mefenoxam is 7:1-1:

5.

2. Use according to claim 1, characterized in that, The mass ratio of the active ingredient A prothioconazole to the active ingredient B mefenoxam is 3:1-3:

4.

3. Use according to claim 1, characterized in that, The content of the active ingredient A and the active ingredient B in the fungicidal composition is 2%-30% based on 100wt% of the total weight of the fungicidal composition.

4. Use according to claim 1, characterized in that, The fungicidal composition further comprises an auxiliary agent selected from one or more of wetting agents, dispersants, emulsifiers, thickening agents, disintegrants, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, warning colors, film formers, synergists and carriers.

5. Use according to claim 1, characterized in that, The dosage form of the fungicidal composition is a seed treatment suspension.

6. Use according to claim 1, characterized in that, The fungicidal composition is applied on a disease or its growth medium in need of control in an effective dose.

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